CN115176139A - 用于调整训练门以适应流式细胞仪数据的方法和系统 - Google Patents

用于调整训练门以适应流式细胞仪数据的方法和系统 Download PDF

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Publication number
CN115176139A
CN115176139A CN202180011892.3A CN202180011892A CN115176139A CN 115176139 A CN115176139 A CN 115176139A CN 202180011892 A CN202180011892 A CN 202180011892A CN 115176139 A CN115176139 A CN 115176139A
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flow cytometry
cytometry data
data
processor
training
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Chinese (zh)
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艾利森·欧文
尼古拉·沙穆西克
约瑟夫·T·特罗特
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Becton Dickinson and Co
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Becton Dickinson and Co
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • G01N33/4915Blood using flow cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402Data analysis by thresholding or gating operations performed on the acquired signals or stored data

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Signal Processing (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN202180011892.3A 2020-01-31 2021-01-21 用于调整训练门以适应流式细胞仪数据的方法和系统 Pending CN115176139A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202062968520P 2020-01-31 2020-01-31
US62/968,520 2020-01-31
US202063049735P 2020-07-09 2020-07-09
US63/049,735 2020-07-09
PCT/US2021/014338 WO2021154579A1 (en) 2020-01-31 2021-01-21 Methods and systems for adjusting a training gate to accommodate flow cytometer data

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CN115176139A true CN115176139A (zh) 2022-10-11

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US (1) US20210278333A1 (https=)
EP (1) EP4097448B1 (https=)
JP (1) JP7663587B2 (https=)
CN (1) CN115176139A (https=)
WO (1) WO2021154579A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12546697B2 (en) 2023-06-23 2026-02-10 Beckman Coulter, Inc. Asynchronous training for classification in flow cytometry

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114441480B (zh) * 2022-01-10 2024-01-26 中国科学院苏州生物医学工程技术研究所 一种有核红细胞分析装置及分析方法
US12529643B2 (en) * 2022-10-31 2026-01-20 Cellcarta Fremont Llc System and method for automatic gating in flow cytometry

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170061657A1 (en) * 2015-09-02 2017-03-02 Becton, Dickinson And Company Graphics control during flow cytometry event gating
US20180135012A1 (en) * 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
CN110121643A (zh) * 2016-07-15 2019-08-13 美国艾森生物科学公司 用于流式细胞仪的光学检测系统、流式细胞仪系统及其使用方法
US20200018704A1 (en) * 2018-07-11 2020-01-16 Visiongate, Inc. Specimen enrichment for optical tomography cell analysis

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4845653A (en) 1987-05-07 1989-07-04 Becton, Dickinson And Company Method of displaying multi-parameter data sets to aid in the analysis of data characteristics
US4987086A (en) 1987-11-30 1991-01-22 Becton, Dickinson And Company Method for analysis of subpopulations of cells
US5627040A (en) 1991-08-28 1997-05-06 Becton Dickinson And Company Flow cytometric method for autoclustering cells
US5739000A (en) 1991-08-28 1998-04-14 Becton Dickinson And Company Algorithmic engine for automated N-dimensional subset analysis
US5556764A (en) 1993-02-17 1996-09-17 Biometric Imaging, Inc. Method and apparatus for cell counting and cell classification
US5700692A (en) 1994-09-27 1997-12-23 Becton Dickinson And Company Flow sorter with video-regulated droplet spacing
US5643796A (en) 1994-10-14 1997-07-01 University Of Washington System for sensing droplet formation time delay in a flow cytometer
US5602039A (en) 1994-10-14 1997-02-11 The University Of Washington Flow cytometer jet monitor system
US5620842A (en) 1995-03-29 1997-04-15 Becton Dickinson And Company Determination of the number of fluorescent molecules on calibration beads for flow cytometry
US6014904A (en) * 1996-05-09 2000-01-18 Becton, Dickinson And Company Method for classifying multi-parameter data
US6821740B2 (en) 1998-02-25 2004-11-23 Becton, Dickinson And Company Flow cytometric methods for the concurrent detection of discrete functional conformations of PRB in single cells
US6372506B1 (en) 1999-07-02 2002-04-16 Becton, Dickinson And Company Apparatus and method for verifying drop delay in a flow cytometer
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
US6809804B1 (en) 2000-05-11 2004-10-26 Becton, Dickinson And Company System and method for providing improved event reading and data processing capabilities in a flow cytometer
JP2004501358A (ja) 2000-05-11 2004-01-15 ベクトン・ディキンソン・アンド・カンパニー 最適な境界を有する平滑化された多角形を使用して散布図中のクラスタを識別するシステム
US6683314B2 (en) 2001-08-28 2004-01-27 Becton, Dickinson And Company Fluorescence detection instrument with reflective transfer legs for color decimation
US7201875B2 (en) 2002-09-27 2007-04-10 Becton Dickinson And Company Fixed mounted sorting cuvette with user replaceable nozzle
GB2428471A (en) * 2005-07-18 2007-01-31 Mathshop Ltd Flow cytometry
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
US8140300B2 (en) 2008-05-15 2012-03-20 Becton, Dickinson And Company High throughput flow cytometer operation with data quality assessment and control
US8233146B2 (en) 2009-01-13 2012-07-31 Becton, Dickinson And Company Cuvette for flow-type particle analyzer
EP2633284B1 (en) 2010-10-25 2021-08-25 Accuri Cytometers, Inc. Systems and user interface for collecting a data set in a flow cytometer
US8528427B2 (en) 2010-10-29 2013-09-10 Becton, Dickinson And Company Dual feedback vacuum fluidics for a flow-type particle analyzer
US8990047B2 (en) 2011-03-21 2015-03-24 Becton, Dickinson And Company Neighborhood thresholding in mixed model density gating
EP2702133B1 (en) 2011-04-29 2017-08-23 Becton Dickinson and Company Cell sorter system and method
AU2012249634B2 (en) 2011-04-29 2016-01-14 Becton, Dickinson And Company Multi-way sorter system and method
US9423348B2 (en) 2011-06-24 2016-08-23 Becton, Dickinson And Company Absorbance spectrum scanning flow cytometry
US20160223554A1 (en) * 2011-08-05 2016-08-04 Nodality, Inc. Methods for diagnosis, prognosis and methods of treatment
CN103906496B (zh) 2011-09-30 2018-03-06 贝克顿·迪金森公司 流体交换方法与装置
US9933341B2 (en) 2012-04-05 2018-04-03 Becton, Dickinson And Company Sample preparation for flow cytometry
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
WO2014169231A1 (en) 2013-04-12 2014-10-16 Norton Pierce O Automated set-up for cell sorting
JP6518245B2 (ja) 2013-11-13 2019-05-22 ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company 光学撮像システム及びそれを用いた方法
JP6691053B2 (ja) 2014-03-18 2020-04-28 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 無線周波数多重化を用いた並行フローサイトメーター
CN106255873B (zh) * 2014-04-10 2019-06-04 深圳迈瑞生物医疗电子股份有限公司 一种进行自动补偿的方法、装置及相应的流式细胞分析仪
CN104158208A (zh) 2014-07-15 2014-11-19 阳光电源股份有限公司 一种单级光伏并网逆变器及其控制方法和应用
WO2017011626A1 (en) 2015-07-15 2017-01-19 Becton, Dickinson And Company System and method for adjusting cytometer measurements
US10302545B2 (en) 2015-10-05 2019-05-28 Becton, Dickinson And Company Automated drop delay calculation
KR102600559B1 (ko) 2015-10-13 2023-11-10 벡톤 디킨슨 앤드 컴퍼니 다중모드 형광 이미징 유동 세포 계측 시스템
KR102527830B1 (ko) 2016-03-17 2023-05-02 벡톤 디킨슨 앤드 컴퍼니 고효율 형광 유세포 분석기를 사용하는 세포 선별
KR102381176B1 (ko) 2016-05-12 2022-04-01 비디 바이오사이언시스 개선된 이미지 해상도를 갖는 형광 이미징 유세포 분석법
US10685045B2 (en) * 2016-07-15 2020-06-16 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for cluster matching across samples and guided visualization of multidimensional cytometry data
CN109477785B (zh) 2016-09-13 2022-09-27 贝克顿·迪金森公司 具有光学均衡的流式细胞仪
WO2018067209A1 (en) 2016-10-05 2018-04-12 Becton, Dickinson And Company Droplet deflectors and methods for using the same
EP3586104A4 (en) 2017-02-27 2020-12-16 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND THEIR OPERATING PROCEDURES
ES2980676T3 (es) * 2017-05-25 2024-10-02 Flowjo Llc Visualización, análisis comparativo y detección de diferencias automatizada para grandes conjuntos de datos multiparamétricos
US10578542B2 (en) 2017-10-16 2020-03-03 Becton, Dickinson And Company Multi-photon counting for high sensitivity flow cytometer systems and methods for using the same
EP3785016A4 (en) 2018-04-26 2022-02-09 Becton, Dickinson and Company BIEXPONENTIAL TRANSFORMATION FOR PARTICLE SORTER
US10883912B2 (en) * 2018-06-04 2021-01-05 Becton, Dickinson And Company Biexponential transformation for graphics display
US12223753B2 (en) * 2019-04-02 2025-02-11 Deutsches Rheuma-Forschungszentrum Berlin Cytometry data analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180135012A1 (en) * 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
US20170061657A1 (en) * 2015-09-02 2017-03-02 Becton, Dickinson And Company Graphics control during flow cytometry event gating
CN110121643A (zh) * 2016-07-15 2019-08-13 美国艾森生物科学公司 用于流式细胞仪的光学检测系统、流式细胞仪系统及其使用方法
US20200018704A1 (en) * 2018-07-11 2020-01-16 Visiongate, Inc. Specimen enrichment for optical tomography cell analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ALVARO JORGE-PEÑAS 等: "Free Form Deformation–Based Image Registration Improves Accuracy of Traction Force Microscopy", 《PLOS ONE》, vol. 10, no. 12, 7 November 2015 (2015-11-07), pages 1 - 22 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12546697B2 (en) 2023-06-23 2026-02-10 Beckman Coulter, Inc. Asynchronous training for classification in flow cytometry

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JP2023511761A (ja) 2023-03-22
US20210278333A1 (en) 2021-09-09
EP4097448A4 (en) 2023-08-23
EP4097448B1 (en) 2025-11-12
WO2021154579A1 (en) 2021-08-05
JP7663587B2 (ja) 2025-04-16
EP4097448A1 (en) 2022-12-07

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