CN115171916A

CN115171916A - Experimental method, device, computer equipment and storage medium for reagent development

Info

Publication number: CN115171916A
Application number: CN202210858268.3A
Authority: CN
Inventors: 王治才; 张毅立; 肖承亮; 骆俊炜; 植立才; 王政
Original assignee: Guangzhou Labsim Biotech Co Ltd
Current assignee: Guangzhou Labsim Biotech Co Ltd
Priority date: 2022-07-20
Filing date: 2022-07-20
Publication date: 2022-10-11
Anticipated expiration: 2042-07-20
Also published as: CN115171916B

Abstract

The present application relates to an experimental method, apparatus, computer device, storage medium and computer program product for reagent development. The method comprises the following steps: determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; this allows the selection of the desired experimental task without performing unnecessary experimental tasks. Determining an experiment sequence corresponding to each experiment task according to the type of the immunological reagent; and automatically executing each experiment task according to the experiment sequence corresponding to each experiment task and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished. By adopting the method, each experiment task can be executed in sequence according to the experiment type, each experiment task can be automatically completed according to the set experiment sequence, and the development efficiency of the immune reagent can be improved.

Description

Reagent development experimental method, device, computer equipment and storage medium

Technical Field

The present application relates to the field of reagent development technologies, and in particular, to an experimental method, an experimental apparatus, a computer device, a storage medium, and a computer program product for reagent development.

Background

The existing research and development process of the immunoreagent needs to arrange and combine raw materials of different manufacturers, the experiment has multiple processes which can also be arranged and combined, the experiment parameters of each process can be adjusted and respectively tested, in addition, a plurality of experiment sample arrangement and combination possibly exist, the development process of the immunoreagent combined in this way needs to carry out a large number of experiments, and therefore the combination mode of the immunoreagent meeting the requirements is selected. For example. The method comprises the steps of respectively providing 4 raw materials for raw materials of a reagent, providing 4 concentration point samples for experiments, providing 4 process combinations for the experiments, providing 2 types for the experiment samples, and calculating the manual experiment times to be 4 × 2 × 3=6144 times by using an experiment time calculation formula (the experiment times = the number of the manufacturers, the number of the raw materials, the number of the process combinations, the number of the concentration points and the number of the sample types), so that a large number of manual experiments are required to select a combination mode of individual reagents meeting requirements. However, the experiment tasks to be performed by each immunoreagent are different, and in a large amount of manual experiments, the same group of experiment tasks are usually performed on all immunoreagents, so that unnecessary experiment tasks are performed on some immunoreagents, development efficiency cannot be guaranteed, the unnecessary experiment tasks may cause more influences of artificial uncertainty factors, the artificial uncertainty factors include inconsistent incubation temperature, inconsistent incubation time and sample loading amount in the test process, and the final test result is biased by the errors.

The development process of the prior immunoreagent is time-consuming, labor-consuming and low in efficiency.

Disclosure of Invention

In view of the above, it is necessary to provide an experimental method, an experimental apparatus, a computer device, a computer-readable storage medium, and a computer program product for reagent development, which can improve the efficiency of immunoreagent development.

In a first aspect, the present application provides an experimental method for reagent development. The method comprises the following steps:

determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence;

determining an experiment sequence corresponding to each experiment task according to the type of the immune reagent;

and automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

In one embodiment, determining the type of experiment corresponding to the immunoreagent according to the experimental requirements of the immunoreagent includes:

determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, a curve fitting, a project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test;

determining a task execution sequence according to the target experiment task;

and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

In one embodiment, before determining the experiment sequence corresponding to each experiment task according to the type of the immunoreagent, the method further includes:

obtaining the type of the immunological reagent, wherein the type of the immunological reagent comprises a fluorescence immunological reagent or a chemiluminescence immunological reagent.

In one embodiment, the determining the experimental order corresponding to each experimental task according to the type of the immunoreagent comprises:

if the type of the immune reagent is a fluorescence method immune reagent, acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence method immune reagent;

and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the first experimental parameters corresponding to the fluorescent immunoreagent include: testing sampling position, testing times, sample number, sample type, incubation type, first testing time, testing time interval and experimental sample concentration;

the determination mode of the first experiment parameter combination mode comprises the following steps:

optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoreagent;

and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode.

if the type of the immunological reagent is a chemiluminescence method immunological reagent, acquiring a raw material combination mode, a flow combination mode and an experiment parameter combination mode corresponding to the chemiluminescence method immunological reagent;

and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the chemiluminescent immunoassay reagent corresponds to a process comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test;

the determination method of the flow combination mode comprises the following steps:

optionally selecting at least one process from the processes corresponding to the chemiluminescence method immunoreagent;

and taking a combination mode formed by at least one flow as a flow combination mode.

In one embodiment, the second experimental parameters for the chemiluminescent immunoreagent include: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters;

the determination mode of the second experiment parameter combination mode comprises the following steps:

optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent;

and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode.

In a second aspect, the present application also provides an experimental apparatus for reagent development. The device comprises:

the type determining module is used for determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence;

the sequence setting module is used for determining the experiment sequence corresponding to each experiment task according to the type of the immunoreagent;

and the automatic experiment module is used for automatically executing all the experiment tasks according to the experiment sequence corresponding to all the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after all the experiment tasks corresponding to the experiment types are executed.

In a third aspect, the present application also provides a computer device. The computer device comprises a memory storing a computer program and a processor implementing the following steps when executing the computer program:

and automatically executing each experiment task according to the experiment sequence corresponding to each experiment task and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

In a fourth aspect, the present application further provides a computer-readable storage medium. The computer-readable storage medium having stored thereon a computer program which, when executed by a processor, performs the steps of:

In a fifth aspect, the present application further provides a computer program product. The computer program product comprising a computer program which when executed by a processor performs the steps of:

determining an experiment sequence corresponding to each experiment task according to the type of the immunological reagent;

The experimental method, the experimental device, the computer equipment, the storage medium and the computer program product for reagent development determine the experimental type corresponding to the immunological reagent according to the experimental requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; this allows the selection of desired experimental tasks without performing unnecessary experimental tasks. Determining an experiment sequence corresponding to each experiment task according to the type of the immune reagent; and automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished. And each experiment task is executed in sequence according to the experiment type, and can be automatically completed according to the set experiment sequence, so that the development efficiency of the immunoreagent can be improved.

Drawings

FIG. 1 is a schematic flow diagram of an experimental method for reagent development in one embodiment;

FIG. 2 is a block diagram showing the structure of an experimental apparatus for reagent development in one embodiment;

FIG. 3 is a diagram illustrating an internal structure of a computer device according to an embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

In one embodiment, as shown in fig. 1, an experimental method for reagent development is provided, and this embodiment is illustrated by applying the method to a computer device, and it is understood that the computer device may be specifically a terminal or a server. The terminal can be but not limited to various personal computers, notebook computers, smart phones, tablet computers, internet of things equipment and portable wearable equipment, and the internet of things equipment can be smart sound boxes, smart televisions, smart air conditioners, smart medical equipment and the like. The portable wearable device can be a smart watch, a smart bracelet, a head-mounted device, and the like. The server may be implemented as a stand-alone server or as a server cluster comprised of multiple servers. In this embodiment, the method includes the steps of:

step 102, determining an experiment type corresponding to an immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence.

The immunological reagent is a reagent for detecting some disease-related markers (antibodies or antigens) in samples of human or animal blood, saliva, urine and the like, which are prepared by labeling antigens or antibodies by using some chemical techniques. The task execution sequence refers to the execution sequence of a plurality of experimental tasks in one experimental type, and if one experimental type only contains one experimental task, the task execution sequence is not required to be considered.

Optionally, the experimental requirements are determined according to the function, use, risk, etc. of the currently developed immunoreagent. The computer equipment selects a proper experiment type from a plurality of pre-configured experiment types according to the experiment requirement.

In an optional embodiment, in the computer device, one or more experiment tasks are combined in advance to obtain a plurality of experiment types, and a task execution sequence is set for the experiment types including the plurality of experiment tasks to obtain a plurality of experiment types configured in advance. For example, the first experiment type contains only experiment task a; the second experiment type only comprises an experiment task B, the third experiment type only comprises an experiment task C, the fourth experiment type only comprises an experiment task D, the fifth experiment type comprises an experiment task A and an experiment task D, and the task execution sequence is set to be AD; the sixth experiment type comprises an experiment task B, an experiment task C and an experiment task D, and the task execution sequence is set to be BCD; the seventh experiment type comprises an experiment task A, an experiment task B, an experiment task C and an experiment task D, and the task execution sequence is set to be ABCD; the eighth experimental type comprises 8230 \ 8230;. The computer equipment selects a proper experiment type from the experiment types such as a first experiment type, a second experiment type, a third experiment type, a fourth experiment type, a fifth experiment type, a sixth experiment type, a seventh experiment type and the like according to the experiment requirement of current reagent development.

And step 104, determining an experiment sequence corresponding to each experiment task according to the type of the immunological reagent.

The type of the immunological reagent comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent. The chemiluminescence immunoassay method is an analysis method combining chemiluminescence and immunoreaction, and comprises an immunochemical reaction part and a chemiluminescence reaction part, wherein the immunochemical reaction part is used for marking chemiluminescence or enzyme on an antigen or an antibody and forming an antigen-antibody immune complex through antigen or antibody specific reaction, the chemiluminescence reaction part is used for adding an oxidant and an alkaline substance (namely an acridine lipid method) or a luminous substrate of enzyme (namely an enzymatic method) after the immunoreaction is finished, the chemiluminescence reaction part emits photons to release energy to return to a stable ground state after being oxidized by the oxidant or catalyzed by the enzyme in an alkaline environment, the luminous intensity can be detected by using a luminous signal measuring instrument, and the chemiluminescence immunoassay reagent realizes the purpose of detecting the concentration of a substance to be detected according to the relation between the luminous intensity and the concentration of the substance to be detected. The fluorescence immunoassay reagent is a reagent for immunoassay by adopting a fluorescence immunoassay technology, and the fluorescence immunoassay technology takes a specific antibody or antigen marked by a fluorescent substance as a standard reagent and is mainly used for analysis, identification and quantitative determination of the corresponding antigen or antibody.

Optionally, the computer device obtains the type of the immunoreagent, where the type of the immunoreagent includes a fluorescence method immunoreagent or a chemiluminescence method immunoreagent, and then determines an experiment sequence corresponding to each experiment task according to the type of the immunoreagent. Because the detection methods of different types of immunoreagents are different, different experimental sequences are set for different types of immunoreagents for each experimental task respectively. An experimental sequence refers to a specific sequence of experimental manipulations in an experimental task, including, but not limited to, for example, adding a sample, adding a reagent, mixing, separating, testing, and the like.

And 106, automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after all the experiment tasks corresponding to the experiment types are executed.

Optionally, the computer device starts the experiment of reagent development according to the experiment sequence, automatically executes each experiment task corresponding to the current experiment type according to the task execution sequence, executes each experiment task according to the corresponding experiment sequence, generates corresponding experiment task statistical information when each experiment task is completed, and outputs the final experiment result of the immunoreagent after all experiment tasks corresponding to the experiment type are executed.

In the experimental method for reagent development, the experimental type corresponding to the immunological reagent is determined according to the experimental requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; this allows the selection of the desired experimental task without performing unnecessary experimental tasks. Determining an experiment sequence corresponding to each experiment task according to the type of the immune reagent; and automatically executing each experiment task according to the experiment sequence corresponding to each experiment task and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished. And each experiment task is executed in sequence according to the experiment type, and can be automatically completed according to the set experiment sequence, so that the development efficiency of the immunoreagent can be improved.

In one embodiment, determining the type of experiment corresponding to the immunoreagent according to the experimental requirements of the immunoreagent comprises: determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, curve fitting, project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

Optionally, it is determined that the immunoreagent needs to perform one or more experimental tasks of the platform period test, the project experiment, the project evaluation and the stability test according to the experimental requirements of the immunoreagent, and then one or more experimental tasks are performed according to the sequence of the platform period test, the project experiment, the project evaluation and the stability test, and the next experimental task is performed in sequence without skipping the performed experimental task. For example, if the experiment needs to be carried out only in the platform period test, the experiment type is determined to be the platform period test; if the experiment only needs to be carried out, determining the experiment type as a project experiment; if the experiment only needs to carry out a project evaluation experiment, determining the type of the experiment as project evaluation; if the experiment only needs to carry out stability test, determining the experiment type as the stability test; if the experiment requires to carry out platform period test and project experiment, determining that the experiment type is platform period test plus project experiment, and the task execution sequence is platform period test plus project experiment; if the experiment needs to carry out platform period test, project experiment and project evaluation, determining that the experiment type is platform period test, project experiment and project evaluation, and the task execution sequence is platform period test, project experiment and project evaluation; and if the experiment needs to develop project experiments and project evaluation, determining the experiment type as project experiments and project evaluation, and determining the task execution sequence as project experiments and project evaluation.

In one possible embodiment, the experimental task includes at least one of a plateau test, a project experiment, a curve fitting, a project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test, a stability test. The platform phase test is mainly used for testing the platform phase of an immune reagent luminescence curve, the project experiment is mainly used for testing the precision of test points with different concentrations of an experiment combination corresponding to an immune reagent so as to confirm the authenticity of a test result, the curve fitting is mainly used for fitting the test result of the immune reagent to form an immune reagent standard curve, the project evaluation is mainly used for verifying the accuracy of the fitting result of a non-experiment point of the immune reagent luminescence curve obtained by fitting, the sensitivity test is mainly used for evaluating the sensitivity of the reagent, the specificity test is mainly used for evaluating the influence degree of endogenous and exogenous interference on the reagent test result, the precision test is mainly used for evaluating the dispersion degree of a plurality of times of measurement results of a fixed sample using the reagent, the linear correlation test is mainly used for evaluating the correlation between the reagent measurement result and a reference value, the accuracy test is mainly used for evaluating the deviation between the reagent test result and the reference value, and the stability test is mainly used for verifying that the deviation of the test result obtained by the experiment combination corresponding to the available immune reagent obtained by the previous test and changing along with time meets the requirement of the accuracy.

In the embodiment, at least one target experiment task to be executed by the immunological reagent is determined according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, curve fitting, project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence. The method can only execute the experiment task meeting the experiment requirement according to the experiment type, and improve the development efficiency of the immunological reagent.

In one embodiment, the determination of the assay sequence corresponding to each assay task, based on the type of immunoreagent, includes: if the type of the immunological reagent is the fluorescence immunological reagent, acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence immunological reagent; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

Further, the determining method of the first experiment parameter combination method includes: optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoreagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode. The first experimental parameters corresponding to the fluorescence immunoassay reagent include: test sampling position, test times, sample number, sample type, incubation type, first test time, test time interval, and experimental sample concentration.

The test sampling position is configured according to user requirements and comprises a test tube rack or an emergency treatment position, and the concentration of the experimental sample comprises high concentration, medium concentration or low concentration.

Optionally, if the type of the immunological reagent is a fluorescence immunological reagent, the experimental sequence of each experimental task includes:

(1) Arranging a plurality of reagent cards according to a certain sequence to obtain a reagent card combination mode;

(2) Selecting a sample type: selecting the type of the sample to be tested according to the type of the sample to be tested;

(3) Configuring the test sampling position (a test tube rack/an emergency position), the test times, the sample number, the sample type, the incubation type, the first test time, the test time interval, the test sample concentration (high, medium and low) and other test sequences according to the user requirements to obtain a first test parameter combination mode; and integrally combining and sequencing the reagent card combination mode and the first experiment parameter combination mode, and setting an experiment sequence.

(4) And loading the reagent card of the item to be tested into the card bin.

(5) And loading the buffer solution matched with the item to be detected into the buffer solution cabin.

(6) The reagent card used is associated with the buffer, a unique reagent number is generated or assigned, and a component list of the combination is entered.

In this embodiment, if the type of the immunoreagent is a fluorescence method immunoreagent, a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence method immunoreagent are obtained; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task. According to the reagent card and the experimental parameters used in the development of the fluorescence immunoassay reagent, reagent developers can customize the combination of the reagent card and the experimental parameters according to the requirements of an experimental scheme, the experimental sequence is combined for each experimental task, each experimental task is executed according to the experimental sequence, and the reagent development efficiency is improved.

In one embodiment, the determination of the assay sequence for each assay task, based on the type of immunoreagent, includes: if the type of the immunological reagent is the chemiluminescent immunological reagent, acquiring a raw material combination mode, a process combination mode and an experimental parameter combination mode corresponding to the chemiluminescent immunological reagent; and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

Further, the determining method of the process combination method includes: optionally selecting at least one process from processes corresponding to chemiluminescence method immunoreagents; and taking the combination mode formed by at least one flow as the flow combination mode. The corresponding process of the chemiluminescence method immunoreagent comprises the following steps: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test.

And the determination mode of the combination mode of the second experiment parameters comprises the following steps: optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode. The second experimental parameters corresponding to the chemiluminescent immunoreagent include: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters;

the sample liquid volume is the sample liquid volume added into the reaction cup, the diluent liquid volume is the diluent liquid volume added into the reaction cup, the reagent parameters comprise the name of a reagent added into the reaction cup, the corresponding bottle number and the reagent liquid volume, shaking, incubation and single magnetic separation, the number of dilution and mixing is the number of dilution and mixing by using a mixing structure, the number of sample shaking is the number of sample shaking by using a shaking structure, the sample incubation time is the sample incubation time by using an incubation disc, the number of single magnetic separation is the number of magnetic separation by using a single magnetic separation assembly (related to a reagent component), the number of continuous magnetic separation is the number of magnetic separation by using a magnetic separation disc, the number of substrate shaking is the number of sample shaking by using a substrate shaking structure, the substrate incubation time is the sample incubation time by using a substrate incubation disc, the first enzymatic test parameter comprises the substrate type, the substrate and the liquid volume test time when an experiment is carried out by using a first enzymatic method, and the second enzymatic test parameter comprises the substrate type, the substrate liquid volume and the test time when a direct luminescent method is used.

Optionally, if the type of the immunological reagent is a chemiluminescent immunological reagent, the experimental sequence of each experimental task includes:

(1) And aiming at one or more raw materials of one or more manufacturers, arranging and combining the raw materials, and arranging an experiment sequence to obtain a raw material combination mode.

For example: 4 raw materials are required, including: m (magnetic bead antibody), R1 (blocking agent), R2 (marker antibody), R3 (biotin antibody), and 4 raw materials (differentiated by suffix 1, 2, 3, and 4) from multiple manufacturers need to be cross-combined to form several sets of reagent sets, for example: the antibody may be selected from the group consisting of a magnetic bead antibody 1+ blocker 1+ marker antibody 1+ biotin antibody 1, a magnetic bead antibody 2+ blocker 1+ marker antibody 1+ biotin antibody 1, a magnetic bead antibody 1+ blocker 2+ marker antibody 1+ biotin antibody 1, a magnetic bead antibody 2+ blocker 2+ marker antibody 1+ biotin antibody 1, a magnetic bead antibody 3+ blocker 2+ marker antibody 1+ biotin antibody 2, and the like. If there are 10 suppliers for each raw material, 10000 possible combinations of 10 × 10 coexist.

Without considering the flow combination manner and the experiment parameter combination manner, the experiment sequence can be set according to certain rules directly according to the 10000 possible manners.

(2) And establishing a new flow according to the requirement or selecting the flow needing to be tested from the following flow list. The flow sequence and the repeated number can be freely configured, and the experimental sequence is set according to a certain rule by combining various possible flows to obtain a flow combination mode.

a) Adding a sample: setting the amount of the sample liquid added into the reaction cup;

b) Adding a diluent: setting the amount of diluent added into the reaction cup;

c) Adding a reagent: setting the name, the corresponding bottle number and the liquid amount of a reagent added into the reaction cup;

d) Diluting and uniformly mixing: setting the times of diluting and mixing by using a mixing structure;

e) Shaking up the sample: setting the number of times of shaking the sample by using the shaking structure;

f) Sample incubation: setting the sample incubation time by using an incubation disc;

g) Single magnetic separation: setting the number of times of magnetic separation by using the single magnetic separation component;

h) Continuous magnetic separation: setting the number of times of magnetic separation by using a magnetic separation disc;

i) Shaking the substrate evenly: setting the times of shaking up the sample by using a substrate shaking-up structure;

j) Substrate incubation: setting the time for sample incubation using the substrate incubation tray;

k) Enzymatic 1 test: setting the type of substrate, the amount of substrate liquid and the test time when the enzymatic method 1 is used for testing;

l) enzymatic 2 assay: setting the substrate type, the substrate liquid amount and the test time when the enzymatic method 2 is used for testing;

m) direct luminescence test: the type of substrate, the amount of substrate liquid, and the test time when the direct luminescence method is used are set.

For example: chemiluminescent, the procedure may include, adding sample-adding diluent-mixing-adding reagent component M-adding reagent component R1-adding reagent component R2-shaking-incubating-magnetically separating-adding substrate-shaking-incubating-detecting, or adding sample-adding reagent component M-adding reagent component R1-adding reagent component R2-adding reagent component R3-shaking-incubating-magnetically separating-adding substrate-shaking-incubating-detecting, etc. The different process sequences are in one combination, and there are thousands of possible process combinations in total.

Without considering the combination mode of raw materials and the combination mode of experimental parameters, the experimental sequence can be set according to a certain rule directly according to the thousands of possible flow combinations.

(3) And establishing parameters according to the requirements or selecting second experiment parameters needing to be tested from the parameter list.

(4) Each flow may individually configure its parameters at execution time. For example:

the ratio parameters for the 4 raw materials form various combinations, e.g. reagent component M (50 μ l) -reagent component R1 (100 μ l) -reagent component R2 (50 μ l), or reagent component M (50 μ l) -reagent component R1 (50 μ l) -reagent component R2 (50 μ l) and so on;

b.4 runs performed with different parameters (interval t between runs, etc.) may form various combinations, such as shaking for 3 or 4 seconds, incubation for 5 or 10 minutes, magnetic separation 3 or 4 times, etc.;

the above procedures and parameters can be combined at will, for example, when each non-mutually exclusive sub-procedure in the test procedure is executed once and the parameters are the same, the type of the procedure is a (11, 11), that is, 39916800; for example, 11 steps are fixed in the sub-flow in the test flow, each parameter has 2 types, and the flow type has 2^11, namely 2048 types;

without considering the combination mode of raw materials and the combination mode of experimental parameters, the experimental sequence can be set according to a certain rule directly according to the possible combination of the procedures and the second experimental parameters.

(5) And randomly combining the raw material combination mode, the process combination mode and the sub-process parameter combination mode.

For example: four raw materials of M magnetic bead antibody 2+ R1 blocker 1+ R2 marker antibody 1+ R3 biotin antibody 1 are used, the process of adding a sample, adding a diluent, uniformly mixing, adding a reagent component M, adding a reagent component R1, adding a reagent component R2, adding a reagent component R3, shaking up, incubating, magnetically separating, adding a substrate, shaking up, incubating and detecting is adopted, wherein 50 microliters of the sample, 150 microliters of the diluent, 50 microliters of the reagent component M, 100 microliters of the reagent component R1, 50 microliters of the reagent component R2, 50 microliters of the reagent component R3 (50 microliters) are added, shaking up is carried out for 3 seconds, magnetic separation is carried out for 3 times after incubation is carried out for 5 minutes, 200 microliters of the substrate is added, shaking up is carried out for 3 seconds, and detection is carried out after incubation is carried out for 5 minutes. The above is a complete experimental combination of raw materials + process + parameters.

And setting the experiment sequence of the experiment combination according to a certain rule by arbitrarily combining the raw material combination mode, the process combination mode and the sub-process parameter combination mode.

In a possible embodiment, the determination of the experimental sequence may include the following cases:

(1) And fixing a flow combination mode and a second experiment parameter combination mode, and only sequencing the combination modes of the multiple raw materials to form an experiment sequence.

(2) And fixing a raw material combination mode and a second experiment parameter combination mode, and only sequencing the multiple process combination modes to form an experiment sequence.

(3) And fixing a raw material combination mode and a flow combination mode, and sequencing the multiple second experiment parameter combination modes to form an experiment sequence.

(4) And fixing one combination mode, combining the other combination mode to form a plurality of experimental combinations, and sequencing to form an experimental sequence. For example, a raw material combination mode is fixed, the flow combination mode and the second experiment parameter combination mode are combined and sequenced to form a plurality of experiment combinations, and the experiment combinations are sequenced to form an experiment sequence.

(5) The three combination modes are all unfixed, the three combination modes are combined randomly to form a plurality of experimental combinations, and the experimental combinations are sequenced to form an experimental sequence.

In this embodiment, if the type of the immunoreagent is a chemiluminescence method immunoreagent, a raw material combination mode, a process combination mode and an experiment parameter combination mode corresponding to the chemiluminescence method immunoreagent are obtained; and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task. The method can automatically perform combination and sequencing according to raw materials, processes and experimental parameters used in the development of the fluorescence immunoassay immunoreagent to generate an experimental sequence, and perform various experimental tasks according to the experimental sequence, thereby improving the efficiency of reagent development.

In one embodiment, an experimental method of reagent development, comprises:

determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, curve fitting, project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence. The experiment type corresponds to at least one experiment task and a task execution sequence.

If the type of the immunological reagent is a fluorescence immunological reagent, selecting at least one first experiment parameter from first experiment parameters corresponding to the fluorescence immunological reagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode. The first experimental parameters corresponding to the fluorescence immunoassay reagent include: test sampling position, test times, sample number, sample type, incubation type, first test time, test time interval, and experimental sample concentration. Acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence immunoassay immunoreagent; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

If the type of the immunological reagent is a chemiluminescence immunological reagent, at least one of the processes corresponding to the chemiluminescence immunological reagent is selected; and taking a combination mode formed by at least one flow as a flow combination mode. The corresponding process of the chemiluminescence method immunoreagent comprises the following steps: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test. Optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode. The second experimental parameters corresponding to the chemiluminescent immunoreagent include: the kit comprises a sample liquid amount, a diluent liquid amount, a reagent parameter, a dilution and uniform mixing frequency, a sample shaking frequency, a sample incubation time, a single magnetic separation frequency, a continuous magnetic separation frequency, a substrate shaking frequency, a substrate incubation time, a first enzymatic test parameter, a second enzymatic test parameter and a direct luminescence test parameter. Acquiring a raw material combination mode, a flow combination mode and an experiment parameter combination mode corresponding to the chemiluminescence method immunoreagent; and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

It should be understood that, although the steps in the flowcharts related to the embodiments as described above are sequentially displayed as indicated by arrows, the steps are not necessarily performed sequentially as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least a part of the steps in the flowcharts related to the embodiments described above may include multiple steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, and the execution order of the steps or stages is not necessarily sequential, but may be rotated or alternated with other steps or at least a part of the steps or stages in other steps.

Based on the same inventive concept, the embodiment of the present application further provides a reagent development experimental apparatus for implementing the above-mentioned reagent development experimental method. The solution scheme provided by the device is similar to the solution scheme described in the above method, so the specific limitations in one or more embodiments of the experimental device for reagent development provided below can be referred to the limitations in the above experimental method for reagent development, and are not described herein again.

In one embodiment, as shown in fig. 2, there is provided a reagent development assay device 200 comprising: a type determination module 201, a sequence setting module 202, and an automatic experiment module 203, wherein:

the type determining module 201 is configured to determine an experiment type corresponding to the immunoreagent according to an experiment requirement of the immunoreagent; the experiment type corresponds to at least one experiment task and a task execution sequence;

the sequence setting module 202 is used for determining an experiment sequence corresponding to each experiment task according to the type of the immunoreagent;

the automatic experiment module 203 is configured to automatically execute each experiment task according to an experiment sequence corresponding to each experiment task and a task execution sequence, and output an experiment result of the immunoreagent after all experiment tasks corresponding to the experiment type are executed.

In one embodiment, the type determining module 201 is further configured to determine at least one target experiment task to be performed by the immunoreagent according to experiment requirements of the immunoreagent, where the experiment task includes a platform period test, a project experiment, a curve fitting, a project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test, and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

In one embodiment, the sequence setting module 202 is further configured to obtain a type of immunoreagent, where the type of immunoreagent includes a fluorescent immunoreagent or a chemiluminescent immunoreagent.

In one embodiment, the sequence setting module 202 is further configured to, if the type of the immunoreagent is a fluorescence immunoassay, obtain a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence immunoassay; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the first experimental parameters for the fluorescent immunoreagent include: testing sampling position, testing times, sample number, sample type, incubation type, first testing time, testing time interval and experimental sample concentration; the sequence setting module 202 is further configured to select at least one first experiment parameter from the first experiment parameters corresponding to the fluorescent immunoassay reagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode.

In one embodiment, the sequence setting module 202 is further configured to, if the type of the immunoreagent is a chemiluminescence method immunoreagent, obtain a raw material combination manner, a process combination manner, and an experiment parameter combination manner corresponding to the chemiluminescence method immunoreagent; and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the chemiluminescent immunoreagent corresponds to a process comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test; the sequence setting module 202 is further configured to select at least one process from processes corresponding to chemiluminescent immunoreagents; and taking the combination mode formed by at least one flow as the flow combination mode.

In one embodiment, the second experimental parameters for the chemiluminescent immunoreagent include: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters; the sequence setting module 202 is further configured to select at least one second experiment parameter from second experiment parameters corresponding to the chemiluminescent immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode.

The modules in the experimental device for reagent development can be wholly or partially realized by software, hardware and a combination thereof. The modules can be embedded in a hardware form or independent of a processor in the computer device, and can also be stored in a memory in the computer device in a software form, so that the processor can call and execute operations corresponding to the modules.

In one embodiment, a computer device is provided, which may be a server, the internal structure of which may be as shown in fig. 3. The computer device includes a processor, a memory, an Input/Output interface (I/O for short), and a communication interface. The processor, the memory and the input/output interface are connected through a system bus, and the communication interface is connected to the system bus through the input/output interface. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The database of the computer equipment is used for storing data such as experiment types, experiment sequences and the like. The input/output interface of the computer device is used for exchanging information between the processor and an external device. The communication interface of the computer device is used for connecting and communicating with an external terminal through a network. The computer program is executed by a processor to implement an experimental method of reagent development.

It will be appreciated by those skilled in the art that the configuration shown in fig. 3 is a block diagram of only a portion of the configuration associated with the present application, and is not intended to limit the computing device to which the present application may be applied, and that a particular computing device may include more or fewer components than shown, or may combine certain components, or have a different arrangement of components.

In one embodiment, a computer device is provided, comprising a memory and a processor, the memory having a computer program stored therein, the processor implementing the following steps when executing the computer program: determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; determining an experiment sequence corresponding to each experiment task according to the type of the immune reagent; and automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

In one embodiment, the processor, when executing the computer program, further performs the steps of: determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, a curve fitting, a project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

In one embodiment, the processor when executing the computer program further performs the steps of: obtaining the type of the immunological reagent, wherein the type of the immunological reagent comprises a fluorescence immunological reagent or a chemiluminescence immunological reagent.

In one embodiment, the processor, when executing the computer program, further performs the steps of: if the type of the immune reagent is a fluorescence method immune reagent, acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence method immune reagent; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the first experimental parameters for the fluorescent immunoreagent include: testing sampling position, testing times, number of samples, sample type, incubation type, first testing time, testing time interval and experimental sample concentration; the processor, when executing the computer program, further performs the steps of: optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoassay reagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode.

In one embodiment, the processor, when executing the computer program, further performs the steps of: if the type of the immunological reagent is a chemiluminescence method immunological reagent, acquiring a raw material combination mode, a flow combination mode and an experiment parameter combination mode corresponding to the chemiluminescence method immunological reagent; and combining and sequencing the raw material combination mode, the process combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the chemiluminescent immunoreagent corresponds to a process comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test; the processor, when executing the computer program, further performs the steps of: optionally selecting at least one process from the processes corresponding to the chemiluminescence method immunoreagent; and taking a combination mode formed by at least one flow as a flow combination mode.

In one embodiment, the second experimental parameters for the chemiluminescent immunoreagent include: sample liquid volume, diluent liquid volume, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters; the processor, when executing the computer program, further performs the steps of: optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode.

In one embodiment, a computer-readable storage medium is provided, having a computer program stored thereon, which when executed by a processor, performs the steps of: determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; determining an experiment sequence corresponding to each experiment task according to the type of the immunological reagent; and automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

In one embodiment, the computer program when executed by the processor further performs the steps of: determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, curve fitting, project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

In one embodiment, the computer program when executed by the processor further performs the steps of: obtaining the type of the immunological reagent, wherein the type of the immunological reagent comprises a fluorescence immunological reagent or a chemiluminescence immunological reagent.

In one embodiment, the computer program when executed by the processor further performs the steps of: if the type of the immune reagent is a fluorescence method immune reagent, acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence method immune reagent; and combining and sequencing the reagent card combination mode and the first experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the first experimental parameters for the fluorescent immunoreagent include: testing sampling position, testing times, sample number, sample type, incubation type, first testing time, testing time interval and experimental sample concentration; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoreagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode.

In one embodiment, the computer program when executed by the processor further performs the steps of: if the type of the immunological reagent is the chemiluminescent immunological reagent, acquiring a raw material combination mode, a process combination mode and an experimental parameter combination mode corresponding to the chemiluminescent immunological reagent; and combining and sequencing the raw material combination mode, the flow combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

In one embodiment, the chemiluminescent immunoreagent is provided in a process comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one process from processes corresponding to chemiluminescence method immunoreagents; and taking the combination mode formed by at least one flow as the flow combination mode.

In one embodiment, the second experimental parameters for the chemiluminescent immunoreagent include: sample liquid volume, diluent liquid volume, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode.

In one embodiment, a computer program product is provided, comprising a computer program which when executed by a processor performs the steps of:

determining the experiment type corresponding to the immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence; determining an experiment sequence corresponding to each experiment task according to the type of the immune reagent; and automatically executing the experiment tasks according to the experiment sequence corresponding to the experiment tasks and the task execution sequence, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

In one embodiment, the computer program when executed by the processor further performs the steps of: determining at least one target experiment task to be executed by the immunological reagent according to the experiment requirement of the immunological reagent, wherein the experiment task comprises a platform period test, a project experiment, a curve fitting, a project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test; determining a task execution sequence according to the target experiment task; and determining the experiment type corresponding to the immunological reagent according to the target experiment task and the task execution sequence.

In one embodiment, the first experimental parameters for the fluorescent immunoreagent include: testing sampling position, testing times, sample number, sample type, incubation type, first testing time, testing time interval and experimental sample concentration; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoassay reagent; and taking the combination mode formed by at least one first experiment parameter as the first experiment parameter combination mode.

In one embodiment, the chemiluminescent immunoreagent is provided in a process comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one process from the processes corresponding to the chemiluminescence method immunoreagent; and taking the combination mode formed by at least one flow as the flow combination mode.

In one embodiment, the second experimental parameters for the chemiluminescent immunoreagent include: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters; the computer program when executed by the processor further realizes the steps of: optionally selecting at least one second experimental parameter from second experimental parameters corresponding to the chemiluminescence immunoassay immunoreagent; and taking the combination mode formed by at least one second experiment parameter as the second experiment parameter combination mode.

It should be noted that, the user information (including but not limited to user equipment information, user personal information, etc.) and data (including but not limited to data for analysis, stored data, displayed data, etc.) referred to in the present application are information and data authorized by the user or sufficiently authorized by each party, and the collection, use and processing of the related data need to comply with the relevant laws and regulations and standards of the relevant country and region.

It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above may be implemented by hardware instructions of a computer program, which may be stored in a non-volatile computer-readable storage medium, and when executed, may include the processes of the embodiments of the methods described above. Any reference to memory, database, or other medium used in the embodiments provided herein may include at least one of non-volatile and volatile memory. The nonvolatile Memory may include Read-Only Memory (ROM), magnetic tape, floppy disk, flash Memory, optical Memory, high-density embedded nonvolatile Memory, resistive Random Access Memory (ReRAM), magnetic Random Access Memory (MRAM), ferroelectric Random Access Memory (FRAM), phase Change Memory (PCM), graphene Memory, and the like. Volatile Memory can include Random Access Memory (RAM), external cache Memory, and the like. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM), among others. The databases referred to in various embodiments provided herein may include at least one of relational and non-relational databases. The non-relational database may include, but is not limited to, a block chain based distributed database, and the like. The processors referred to in the embodiments provided herein may be general purpose processors, central processing units, graphics processors, digital signal processors, programmable logic devices, quantum computing based data processing logic devices, etc., without limitation.

All possible combinations of the technical features in the above embodiments may not be described for the sake of brevity, but should be considered as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, and these are all within the scope of protection of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. An assay method for reagent development, said method comprising:

determining an experiment type corresponding to an immunological reagent according to the experiment requirement of the immunological reagent; the experiment type corresponds to at least one experiment task and a task execution sequence;

and automatically executing each experiment task according to the task execution sequence and the experiment sequence corresponding to each experiment task, and outputting the experiment result of the immunoreagent after the execution of all the experiment tasks corresponding to the experiment types is finished.

2. The method of claim 1, wherein the determining the type of the experiment corresponding to the immunoreagent according to the experimental requirement of the immunoreagent comprises:

determining at least one target experiment task to be executed by the immunoreagent according to the experiment requirement of the immunoreagent, wherein the experiment task comprises a platform period test, a project experiment, curve fitting, project evaluation, a sensitivity test, a specificity test, a precision test, a linear correlation test, an accuracy test and a stability test;

determining the task execution sequence according to the target experiment task;

3. The method of claim 1, wherein prior to determining the test sequence corresponding to each test task according to the type of the immunoreagent, the method further comprises:

4. The method of claim 3, wherein determining the experimental order corresponding to each experimental task according to the type of the immunoreagent comprises:

if the type of the immunological reagent is the fluorescence immunological reagent, acquiring a reagent card combination mode and a first experiment parameter combination mode corresponding to the fluorescence immunological reagent;

5. The method of claim 4, wherein the first experimental parameters for the fluorescent immunoreagent comprise: testing sampling position, testing times, number of samples, sample type, incubation type, first testing time, testing time interval and experimental sample concentration;

optionally selecting at least one first experimental parameter from the first experimental parameters corresponding to the fluorescent immunoassay reagent;

and taking the combination mode formed by the at least one first experiment parameter as the first experiment parameter combination mode.

6. The method of claim 3, wherein determining the experimental order corresponding to each experimental task according to the type of the immunoreagent comprises:

if the type of the immunological reagent is the chemiluminescence immunological reagent, acquiring a raw material combination mode, a process combination mode and an experiment parameter combination mode corresponding to the chemiluminescence immunological reagent;

and combining and sequencing the raw material combination mode, the flow combination mode and the experiment parameter combination mode to obtain an experiment sequence corresponding to each experiment task.

7. The method of claim 6, wherein the chemiluminescent immunoreagent is prepared according to a protocol comprising: adding a sample, adding a diluent, adding a reagent, diluting and uniformly mixing, shaking the sample, incubating the sample, performing single magnetic separation, performing continuous magnetic separation, shaking a substrate, incubating the substrate, performing a first enzymatic test, performing a second enzymatic test and performing a direct luminescence test;

the determination mode of the flow combination mode comprises the following steps:

optionally selecting at least one process from the processes corresponding to the chemiluminescent immunoreagent;

and taking the combination mode formed by the at least one flow as the flow combination mode.

8. The method of claim 6, wherein the second experimental parameters for the chemiluminescent immunoreagent comprise: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters;

optionally selecting at least one second experimental parameter from the second experimental parameters corresponding to the chemiluminescent immunoreagent;

and taking the combination mode formed by the at least one second experiment parameter as the second experiment parameter combination mode.

9. An assay device for reagent development, said device comprising:

the sequence setting module is used for determining an experiment sequence corresponding to each experiment task according to the type of the immunoreagent;

10. A computer device comprising a memory and a processor, the memory storing a computer program, characterized in that the processor realizes the steps of the method of any one of claims 1 to 6 when executing the computer program.