CN114400044A - Biomolecule detection method - Google Patents
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- 238000001514 detection method Methods 0.000 title claims abstract description 66
- 238000012360 testing method Methods 0.000 claims abstract description 128
- 238000010839 reverse transcription Methods 0.000 claims abstract description 59
- 238000006243 chemical reaction Methods 0.000 claims abstract description 39
- 238000000926 separation method Methods 0.000 claims abstract description 29
- 239000000523 sample Substances 0.000 claims abstract description 24
- 230000001915 proofreading effect Effects 0.000 claims abstract description 23
- 230000005540 biological transmission Effects 0.000 claims abstract description 22
- 239000012488 sample solution Substances 0.000 claims abstract description 19
- 238000003860 storage Methods 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 294
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 62
- 239000006166 lysate Substances 0.000 claims description 18
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- 238000000034 method Methods 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 210000000056 organ Anatomy 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
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- 102000029816 Collagenase Human genes 0.000 claims description 5
- 108060005980 Collagenase Proteins 0.000 claims description 5
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- 102100034343 Integrase Human genes 0.000 claims description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 4
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- 239000005547 deoxyribonucleotide Substances 0.000 claims description 4
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
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- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
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Abstract
The invention relates to the field of biological detection, and discloses a biomolecule detection method, which comprises the following steps: separating the precipitated solution from the pre-constructed separation layer solution, performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution, preparing molecular test paper to be detected by using the reverse transcription solution, transferring the molecular test paper to be detected to a test paper transmission channel, extracting a proofreading sample solution by using a sample extraction arm, adding the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted, storing the test paper to be reacted in a greenhouse warehouse, standing for whether the test paper to be reacted has a chemical reaction, and determining a detection result of the concentrated solution to be detected according to the chemical reaction if the test paper to be reacted has the chemical reaction. The invention also discloses a biomolecule detection method and device, electronic equipment and a storage medium. The invention can solve the problem of low intelligent degree of biomolecule detection.
Description
Technical Field
The present invention relates to the field of biological detection, and in particular, to a method and an apparatus for detecting biomolecules, an electronic device, and a computer-readable storage medium.
Background
The biomolecule detection is a detection means for detecting the molecular structure in biological cells so as to judge whether the biological cells are mutated or not and whether the pH value is changed or not, and can play a role in early diagnosis, early discovery and early treatment
At present, the main biomolecule detection methods include enzyme-linked immunosorbent assay, chemiluminescence assay and the like, and the biomolecular detection method mainly comprises the steps of directly observing a biological cell tissue through instruments such as a microscope and the like, and researching the reaction change of the biological cell tissue and a chemical reagent by matching with the chemical reagent.
Disclosure of Invention
The invention provides a biomolecule detection method, a biomolecule detection device, electronic equipment and a computer-readable storage medium, and mainly aims to solve the problem of low intelligent degree of biomolecule detection.
In order to achieve the above object, the present invention provides a method for detecting a biomolecule, comprising:
receiving a concentrated solution to be detected, which is prepared in advance by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate in a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be stood;
after shaking the solution to be kept stand, keeping the solution to be kept stand for the first time to obtain a solution to be precipitated;
injecting chloroform liquid into the solution to be precipitated, continuously shaking the solution to be precipitated in which the chloroform liquid is injected, standing the solution to be precipitated for a second time to obtain a separation layer solution, wherein the separation layer solution comprises a precipitated solution;
separating the precipitated solution from the separation layer solution by using ethanol, and performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
preparing molecular test paper to be detected by using the reverse transcription solution, and transferring the molecular test paper to be detected to a test paper transmission channel;
extracting a proofreading sample solution by using a sample extraction arm, and injecting the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
storing the test paper to be reacted in a greenhouse warehouse, standing until whether the test paper to be reacted has a chemical reaction, and returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted has no chemical reaction;
and if the test paper to be reacted has a chemical reaction, determining the detection result of the concentrated solution to be detected according to the chemical reaction.
Optionally, the pre-configured concentrate to be tested comprises:
receiving biological cells extracted in advance by a user, and judging whether the cell types of the biological cells are organ tissue cells or not;
if the cell type of the biological cell is organ tissue cell, placing the biological cell in a pre-prepared collagenase solution to obtain the concentrated solution to be detected;
and if the cell type of the biological cell is not organ tissue cell, placing the biological cell in a pre-configured balanced salt solution to obtain the concentrated solution to be detected.
Optionally, after shaking the solution to be left standing, the solution to be left standing for a first time to obtain a solution to be precipitated, including:
placing the solution to be kept stand in an automatic rocker arm machine, adjusting the vibration frequency and the vibration time of the automatic rocker arm machine, and starting the automatic rocker arm machine;
and when the automatic rocker arm machine stops working, standing the solution to be stood for five minutes to obtain the solution to be precipitated.
Optionally, the adjusting the shaking frequency and the shaking time of the automatic rocker arm machine and starting the automatic rocker arm machine includes:
receiving a shaking program script constructed by a user in advance;
analyzing the shaking program script to obtain the shaking frequency and the shaking time;
and starting the automatic rocker arm machine according to the vibration frequency and the vibration time by utilizing a computer connected with the automatic rocker arm machine.
Optionally, the injecting a chloroform solution into the solution to be precipitated includes:
measuring the solution capacity of the solution to be precipitated;
calculating the solution capacity of the chloroform solution according to the pre-constructed proportion and the solution capacity of the solution to be precipitated;
and injecting a chloroform solution into the solution to be precipitated according to the solution capacity of the chloroform solution.
Optionally, the performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution, comprising:
under the condition of keeping the temperature of the precipitated solution constant, adding a reverse transcription buffer solution, a deoxyribonucleotide mixed solution and a random primer;
after standing for a third time period, adding reverse transcriptase and DEPC water into the precipitated solution to obtain a solution to be reversed;
after allowing the solution to be inverted to stand for a fourth period of time, extracting the reverse transcription solution including cDNA from the solution to be inverted.
Optionally, the preparing the molecular test paper with the reverse transcription solution and transferring the molecular test paper to a test paper transmission channel comprises:
loading the reverse transcription solution into an automatic dropper;
and dripping three drops of reverse transcription solution from the reverse transcription solution to test paper by using the automatic dropper to obtain the test paper to be detected by the molecules.
In order to solve the above problems, the present invention also provides a biomolecule detection device, comprising:
the device comprises a lysate injection module, a sample collection module and a sample analysis module, wherein the lysate injection module is used for receiving a concentrated solution to be detected, which is pre-configured by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate with a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be kept static;
a solution separation module, configured to, after shaking the solution to be left standing, stand the solution to be left standing for a first time to obtain a solution to be precipitated, inject a chloroform solution into the solution to be precipitated, and after continuously shaking the solution to be precipitated into which the chloroform solution is injected, stand the solution to be precipitated for a second time to obtain a separation layer solution, where the separation layer solution includes a precipitated solution, separate the precipitated solution from the separation layer solution using ethanol, and perform a reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
the test paper preparation module is used for preparing molecular test paper to be detected by utilizing the reverse transcription solution, transferring the molecular test paper to be detected to a test paper transmission channel, extracting a proofreading sample liquid by utilizing a sample extraction arm, and filling the proofreading sample liquid into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
and the molecular detection module is used for storing the test paper to be reacted into a greenhouse warehouse, standing whether the test paper to be reacted has a chemical reaction or not, returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted does not have the chemical reaction, and determining the detection result of the concentrated solution to be detected according to the chemical reaction if the test paper to be reacted has the chemical reaction.
In order to solve the above problem, the present invention also provides an electronic device, including:
at least one processor; and the number of the first and second groups,
a memory communicatively coupled to the at least one processor; wherein,
the memory stores instructions executable by the at least one processor to cause the at least one processor to perform the biomolecule detection methods described above.
In order to solve the above problems, the present invention also provides a computer-readable storage medium storing a computer program which, when executed by a processor, implements the above biomolecule detection method.
According to the embodiment of the invention, a microscope is not directly used for observing biological cell tissues, but a concentrated solution to be detected containing biological cells is firstly used for obtaining a reverse transcription solution only containing the biological cells through operations of cracking, shaking, standing, reverse transcription and the like, a molecular test paper to be detected is further prepared by using the reverse transcription solution, a proofreading sample solution is extracted by combining a sample extraction arm, and finally the molecular test paper to be detected and the proofreading sample solution react on a test paper transmission channel. In the whole biomolecule detection process, less intervention of detection personnel is needed, only the chemical reaction between the test paper to be detected and the proofreading sample liquid needs to be observed finally, the cell tissue does not need to be observed repeatedly by using a microscope, and the intelligent degree of the whole detection is improved.
Drawings
FIG. 1 is a schematic flow chart of a biomolecule detection method according to an embodiment of the present invention;
FIG. 2 is a schematic view of the process of S2 in the biomolecule detection method according to an embodiment of the present invention;
FIG. 3 is a schematic view of the process of S3 in the biomolecule detection method according to an embodiment of the present invention;
FIG. 4 is a diagram of an apparatus for implementing a biomolecule detection apparatus according to an embodiment of the present invention;
fig. 5 is a schematic diagram of an internal structure of an electronic device for implementing a biomolecule detection method according to an embodiment of the present invention;
the implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Embodiments of the present invention relate to a biomolecule detection method. The execution subject of the biomolecule detection method provided by the embodiment of the application includes, but is not limited to, at least one of electronic devices such as a server and a terminal, which can be configured to execute the method provided by the embodiment of the application. In other words, the biomolecule detection method can be performed by software or hardware installed in a terminal device or a server device, and the software can be a block chain platform. The server includes but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, and the like.
Referring to fig. 1, a schematic flow chart of a biomolecule detection method according to an embodiment of the present invention is shown. In this embodiment, the biomolecule detection method includes:
s1, receiving a concentrated solution to be detected, which is prepared in advance by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate in a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be kept still.
In the embodiment of the present invention, since biomolecules exist in biological cells and have an important influence on the structure and composition of the biological cells, if the detection is directly performed on the biological cells, since living environments, sizes, storage conditions, and the like of different biological cells are different, the detection difficulty is very high, and therefore, a to-be-detected concentrated solution corresponding to the biological cells needs to be configured in advance, wherein the to-be-detected concentrated solution configured in advance includes:
receiving biological cells extracted in advance by a user, and judging whether the cell types of the biological cells are organ tissue cells or not;
if the cell type of the biological cell is organ tissue cell, placing the biological cell in a pre-prepared collagenase solution to obtain the concentrated solution to be detected;
and if the cell type of the biological cell is not organ tissue cell, placing the biological cell in a pre-configured balanced salt solution to obtain the concentrated solution to be detected.
In a preferred embodiment of the present invention, the biological cells include organ tissue cells, virus cells, plant cells, in vitro cells, etc., and since the organ tissue cells are more suitable for survival in a collagenase solution, when the biological cells are organ tissue cells, the collagenase solution is used to generate the concentrated solution to be detected.
Further, in order to fully understand the information such as the structure of the biomolecule in the biological cell, it is necessary to extract the soluble protein from the biological cell, wherein the lysate reacts with the biological cell, thereby efficiently extracting the soluble protein from the biological cell.
In detail, the corresponding ratio is generally 1:3, if the volume of the to-be-detected concentrated solution is 100 ml, the volume of the lysate is 300 ml, and the to-be-detected concentrated solution and the lysate are fully fused according to the 1:3 volume ratio to obtain the to-be-left-standing solution.
And S2, shaking the solution to be kept stand, and keeping the solution to be kept stand for the first time to obtain a solution to be precipitated.
In order to sufficiently fuse the to-be-detected concentrate and the lysate, the to-be-left solution needs to be repeatedly shaken and left for a certain period of time, and in detail, referring to fig. 2, the S2 includes:
s21, placing the solution to be kept stand in an automatic rocker arm machine, adjusting the vibration frequency and the vibration time of the automatic rocker arm machine, and starting the automatic rocker arm machine;
and S22, standing the solution to be settled for five minutes to obtain the solution to be settled when the automatic rocker arm machine stops working.
In the embodiment of the invention, the automatic rocker arm machine is connected with a computer, and the computer controls the automatic rocker arm machine through an operational program, so that automatic shaking is completed. In detail, the adjusting the vibration frequency and the vibration time of the automatic rocker arm machine and starting the automatic rocker arm machine includes:
receiving a shaking program script constructed by a user in advance;
analyzing the shaking program script to obtain the shaking frequency and the shaking time;
and starting the automatic rocker arm machine according to the vibration frequency and the vibration time by utilizing a computer connected with the automatic rocker arm machine.
Further, when the automatic rocker arm machine stops working, the automatic rocker arm machine finishes shaking the solution to be settled on the basis of the shaking frequency and the shaking time.
S3, injecting chloroform liquid into the solution to be precipitated, continuously shaking the solution to be precipitated injected with the chloroform liquid, and standing the solution to be precipitated for a second time to obtain a separation layer solution, wherein the separation layer solution comprises the precipitated solution.
When the solution to be precipitated is obtained by performing the above-mentioned operation of S2, a large amount of soluble proteins are suspended in the solution to be precipitated, and since the suspended soluble proteins are difficult to extract, it is necessary to precipitate the soluble proteins suspended in the solution to be precipitated at the bottom of the solution to extract the soluble proteins.
Further, the chloroform solution can adsorb soluble proteins suspended in the solution to be precipitated and precipitate at the bottom of the solution, and in the embodiment of the present invention, referring to fig. 3, the injecting of the chloroform solution into the solution to be precipitated includes:
s31, measuring the solution capacity of the solution to be precipitated;
s32, calculating the solution capacity of the chloroform solution according to the pre-constructed proportion and the solution capacity of the solution to be precipitated;
and S33, injecting a chloroform solution into the solution to be precipitated according to the solution capacity of the chloroform solution.
In the embodiment of the invention, the ratio of the solution capacity of the chloroform solution to the solution capacity of the solution to be precipitated is set to 1:5, for example, the solution capacity of the solution to be precipitated is 1000 ml, and the solution capacity of the chloroform solution is 200 ml.
After the solution volume of the chloroform solution is injected, in order to sufficiently combine the chloroform solution with the solution to be precipitated, a period of standing is required, and in the embodiment of the present invention, the second period of time is generally set to 10 minutes to 15 minutes.
The separation layer solution is obtained when left to stand for 10 to 15 minutes, wherein the precipitated solution is generally at the bottom of the separation layer solution.
S4, separating the precipitated solution from the separation layer solution by using ethanol, and performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution.
In the embodiment of the invention, low-alcohol is used, and because low-alcohol is generally difficult to dissolve other solutions and cannot damage soluble proteins in the solutions, when the low-alcohol is injected into the separation layer solution, the low-alcohol can separate the solution containing the soluble proteins from other solutions, and the precipitated solution is obtained.
In detail, the performing a reverse transcription operation on the precipitated solution to obtain a reverse transcription solution includes:
under the condition of keeping the temperature of the precipitated solution constant, adding a reverse transcription buffer solution, a deoxyribonucleotide mixed solution and a random primer;
after standing for a third time period, adding reverse transcriptase and DEPC water into the precipitated solution to obtain a solution to be reversed;
after allowing the solution to be inverted to stand for a fourth period of time, extracting the reverse transcription solution including cDNA from the solution to be inverted.
In the present embodiment, a constant temperature of 40 ° to 50 ° is generally maintained.
In addition, if the amount of the precipitated solution is not more than 20 ml, the amount of the reverse transcription buffer solution is not more than 5 ml, the amount of the deoxyribonucleotide mixture is not more than 1 ml, and the amount of the random primer is not more than 1 ml. Further, the reverse transcriptase amount is not more than 0.25 ml, and the DEPC water amount is not more than 2 ml.
Wherein the third time period is 1-5 minutes, and the fourth time period is 10-15 minutes, and obtaining the reverse transcription solution containing cDNA according to the operation.
S5, preparing molecular test paper to be detected by using the reverse transcription solution, and transferring the molecular test paper to be detected to a test paper transmission channel.
Because different test paper has different test functions, the embodiment of the present invention can manufacture different test paper to be tested for molecules according to the test purpose, in the embodiment of the present invention, the S5 includes:
loading the reverse transcription solution into an automatic dropper;
and dripping three drops of reverse transcription solution from the reverse transcription solution to test paper by using the automatic dropper to obtain the test paper to be detected by the molecules.
For example, in order to test the alkalinity of the reverse transcription solution, the above three drops of reverse transcription solution were received using litmus red paper, in order to test the oxidizing substance in the reverse transcription solution, potassium iodide starch paper, etc. were used.
In the embodiment of the invention, the test paper transmission channel is a conveyor belt for conveying the molecular test paper to be detected according to a preset conveying frequency, and in order to prevent the molecular test paper to be detected from being oxidized, the test paper transmission channel is generally in a vacuum state.
And S6, extracting a proofreading sample solution by using a sample extraction arm, and injecting the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain the test paper to be reacted.
In the embodiment of the present invention, the sample extraction arm is generally built in the test paper transmission channel to form an automated molecular testing apparatus, wherein the calibration sample solution is a liquid to perform a chemical reaction with the test paper to be tested, and thus different calibration sample solutions are extracted correspondingly when different chemical reactions need to be performed, and the calibration sample solution can use a PH detection solution when the reverse transcription solution is subjected to an alkaline detection.
And S7, storing the test paper to be reacted in a greenhouse warehouse, standing until whether the test paper to be reacted has a chemical reaction, and returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted has no chemical reaction.
In the embodiment of the invention, the temperature of the greenhouse warehouse can be set to be 40-50 degrees, when the test paper to be reacted does not have chemical reaction, the detection fails, the molecular test paper to be detected needs to be prepared again, other proofreading sample liquid and the molecular test paper to be detected are used for carrying out chemical reaction, and whether the chemical reaction occurs or not is checked.
And S8, if the test paper to be reacted has a chemical reaction, determining the detection result of the concentrated solution to be detected according to the chemical reaction.
In order to test the pH value of the solution and the color of the test paper to be reacted is changed, the pH value of the concentrated solution to be detected can be determined according to different colors, so that the biomolecule detection is completed.
According to the embodiment of the invention, a microscope is not directly used for observing biological cell tissues, but a concentrated solution to be detected containing biological cells is firstly used for obtaining a reverse transcription solution only containing the biological cells through operations of cracking, shaking, standing, reverse transcription and the like, a molecular test paper to be detected is further prepared by using the reverse transcription solution, a proofreading sample solution is extracted by combining a sample extraction arm, and finally the molecular test paper to be detected and the proofreading sample solution react on a test paper transmission channel. In the whole biomolecule detection process, less intervention of detection personnel is needed, only the chemical reaction between the test paper to be detected and the proofreading sample liquid needs to be observed finally, the cell tissue does not need to be observed repeatedly by using a microscope, and the intelligent degree of the whole detection is improved.
FIG. 4 is a schematic block diagram of the biomolecule detection device of the present invention.
The biomolecule detection devices 100 according to the present invention can be mounted in electronic devices. According to the realized function, the biomolecule detection device can comprise a lysate injection module 101, a solution separation module 102, a test paper preparation module 103 and a molecule detection module 104. A module according to the present invention, which may also be referred to as a unit, refers to a series of computer program segments that can be executed by a processor of an electronic device and that can perform a fixed function, and that are stored in a memory of the electronic device.
In the present embodiment, the functions regarding the respective modules/units are as follows:
the device comprises a lysate injection module 101, a sample collection module and a sample analysis module, wherein the lysate injection module 101 is used for receiving a to-be-detected concentrated solution which is pre-configured by a user, measuring the capacity of the to-be-detected concentrated solution, and injecting a lysate which is in a proportion corresponding to the capacity into the to-be-detected concentrated solution to obtain a to-be-kept solution;
a solution separation module 102, configured to, after shaking the solution to be left standing, stand the solution to be left standing for a first time to obtain a solution to be precipitated, inject a chloroform solution into the solution to be precipitated, and continuously shake the solution to be precipitated into which the chloroform solution is injected, and then stand the solution to be precipitated for a second time to obtain a separation layer solution, where the separation layer solution includes a precipitated solution, separate the precipitated solution from the separation layer solution using ethanol, and perform a reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
the test paper preparation module 103 is used for preparing molecular test paper to be detected by using the reverse transcription solution, transferring the molecular test paper to be detected to a test paper transmission channel, extracting a proofreading sample solution by using a sample extraction arm, and injecting the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
and the molecular detection module 104 is configured to store the test paper to be reacted in a greenhouse warehouse, wait for whether the test paper to be reacted undergoes a chemical reaction, return to the step of preparing the molecular test paper to be detected if the test paper to be reacted does not undergo a chemical reaction, and determine a detection result of the concentrated solution to be detected according to the chemical reaction if the test paper to be reacted undergoes a chemical reaction.
Each module in the biomolecule detection device 100 provided in the embodiment of the present invention can use the same means as the biomolecule detection method, and the specific implementation steps are not described herein again, and the technical effect generated by the functions of each module/unit is the same as the technical effect of the biomolecule detection method, i.e. the problem of low intelligent degree of biomolecule detection is solved.
FIG. 5 is a schematic structural diagram of an electronic device for implementing the biomolecule detection method according to the present invention.
The electronic device 1 may comprise a processor 10, a memory 11 and a bus, and may further comprise a computer program, such as a biomolecule detection method program 12, stored in the memory 11 and executable on the processor 10.
The memory 11 includes at least one type of readable storage medium, which includes flash memory, removable hard disk, multimedia card, card-type memory (e.g., SD or DX memory, etc.), magnetic memory, magnetic disk, optical disk, etc. The memory 11 may in some embodiments be an internal storage unit of the electronic device 1, such as a removable hard disk of the electronic device 1. The memory 11 may also be an external storage device of the electronic device 1 in other embodiments, such as a plug-in mobile hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card), and the like, which are provided on the electronic device 1. Further, the memory 11 may also include both an internal storage unit and an external storage device of the electronic device 1. The memory 11 may be used not only to store application software installed in the electronic device 1 and various types of data, such as codes of the biomolecule detection method program 12, but also to temporarily store data that has been output or is to be output.
The processor 10 may be composed of an integrated circuit in some embodiments, for example, a single packaged integrated circuit, or may be composed of a plurality of integrated circuits packaged with the same or different functions, including one or more Central Processing Units (CPUs), microprocessors, digital Processing chips, graphics processors, and combinations of various control chips. The processor 10 is a Control Unit (Control Unit) of the electronic device, connects various components of the electronic device by using various interfaces and lines, and executes various functions and processes data of the electronic device 1 by running or executing programs or modules (e.g., programs for executing a biomolecule detection method, etc.) stored in the memory 11 and calling data stored in the memory 11.
The bus may be a Peripheral Component Interconnect (PCI) bus, an Extended Industry Standard Architecture (EISA) bus, or the like. The bus may be divided into an address bus, a data bus, a control bus, etc. The bus is arranged to enable connection communication between the memory 11 and at least one processor 10 or the like.
Fig. 5 only shows an electronic device with components, and it will be understood by a person skilled in the art that the structure shown in fig. 5 does not constitute a limitation of the electronic device 1, and may comprise fewer or more components than shown, or a combination of certain components, or a different arrangement of components.
For example, although not shown, the electronic device 1 may further include a power supply (such as a battery) for supplying power to each component, and preferably, the power supply may be logically connected to the at least one processor 10 through a power management device, so as to implement functions of charge management, discharge management, power consumption management, and the like through the power management device. The power supply may also include any component of one or more dc or ac power sources, recharging devices, power failure detection circuitry, power converters or inverters, power status indicators, and the like. The electronic device 1 may further include various sensors, a bluetooth module, a Wi-Fi module, and the like, which are not described herein again.
Further, the electronic device 1 may further include a network interface, and optionally, the network interface may include a wired interface and/or a wireless interface (such as a WI-FI interface, a bluetooth interface, etc.), which are generally used for establishing a communication connection between the electronic device 1 and other electronic devices.
Optionally, the electronic device 1 may further comprise a user interface, which may be a Display (Display), an input unit (such as a Keyboard), and optionally a standard wired interface, a wireless interface. Alternatively, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, an OLED (Organic Light-Emitting Diode) touch device, or the like. The display, which may also be referred to as a display screen or display unit, is suitable for displaying information processed in the electronic device 1 and for displaying a visualized user interface, among other things.
It is to be understood that the described embodiments are for purposes of illustration only and that the scope of the appended claims is not limited to such structures.
The biomolecule detection method program 12 stored in the memory 11 of the electronic device 1 is a combination of instructions that, when executed in the processor 10, enable:
receiving a concentrated solution to be detected, which is prepared in advance by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate in a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be stood;
after shaking the solution to be kept stand, keeping the solution to be kept stand for the first time to obtain a solution to be precipitated;
injecting chloroform liquid into the solution to be precipitated, continuously shaking the solution to be precipitated in which the chloroform liquid is injected, standing the solution to be precipitated for a second time to obtain a separation layer solution, wherein the separation layer solution comprises a precipitated solution;
separating the precipitated solution from the separation layer solution by using ethanol, and performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
preparing molecular test paper to be detected by using the reverse transcription solution, and transferring the molecular test paper to be detected to a test paper transmission channel;
extracting a proofreading sample solution by using a sample extraction arm, and injecting the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
storing the test paper to be reacted in a greenhouse warehouse, standing until whether the test paper to be reacted has a chemical reaction, and returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted has no chemical reaction;
and if the test paper to be reacted has a chemical reaction, determining the detection result of the concentrated solution to be detected according to the chemical reaction.
Further, the integrated modules/units of the electronic device 1, if implemented in the form of software functional units and sold or used as separate products, may be stored in a computer readable storage medium. The computer-readable medium may include: any entity or device capable of carrying said computer program code, recording medium, U-disk, removable hard disk, magnetic disk, optical disk, computer Memory, Read-Only Memory (ROM).
Further, the computer usable storage medium may mainly include a storage program area and a storage data area, wherein the storage program area may store an operating device, an application program required for at least one function, and the like; the storage data area may store data created according to the use of the blockchain node, and the like.
In the embodiments provided in the present invention, it should be understood that the disclosed apparatus, device and method can be implemented in other ways. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the modules is only one logical functional division, and other divisions may be realized in practice.
The modules described as separate parts may or may not be physically separate, and parts displayed as modules may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment.
In addition, functional modules in the embodiments of the present invention may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional module.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.
The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any accompanying claims should not be construed as limiting the claim concerned.
Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. A plurality of units or means recited in the apparatus claims may also be implemented by one unit or means in software or hardware. The terms second, etc. are used to denote names, but not any particular order.
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A method for detecting a biomolecule, the method comprising:
receiving a concentrated solution to be detected, which is prepared in advance by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate in a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be stood;
after shaking the solution to be kept stand, keeping the solution to be kept stand for the first time to obtain a solution to be precipitated;
injecting chloroform liquid into the solution to be precipitated, continuously shaking the solution to be precipitated in which the chloroform liquid is injected, standing the solution to be precipitated for a second time to obtain a separation layer solution, wherein the separation layer solution comprises a precipitated solution;
separating the precipitated solution from the separation layer solution by using ethanol, and performing reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
preparing molecular test paper to be detected by using the reverse transcription solution, and transferring the molecular test paper to be detected to a test paper transmission channel;
extracting a proofreading sample solution by using a sample extraction arm, and injecting the proofreading sample solution into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
storing the test paper to be reacted in a greenhouse warehouse, standing until whether the test paper to be reacted has a chemical reaction, and returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted has no chemical reaction;
and if the test paper to be reacted has a chemical reaction, determining the detection result of the concentrated solution to be detected according to the chemical reaction.
2. The method for detecting biomolecules according to claim 1, wherein the pre-prepared concentrate to be detected comprises:
receiving biological cells extracted in advance by a user, and judging whether the cell types of the biological cells are organ tissue cells or not;
if the cell type of the biological cell is organ tissue cell, placing the biological cell in a pre-prepared collagenase solution to obtain the concentrated solution to be detected;
and if the cell type of the biological cell is not organ tissue cell, placing the biological cell in a pre-configured balanced salt solution to obtain the concentrated solution to be detected.
3. The method for detecting biomolecules according to claim 1, wherein the shaking the solution to be allowed to stand for a first time to obtain a solution to be precipitated comprises:
placing the solution to be kept stand in an automatic rocker arm machine, adjusting the vibration frequency and the vibration time of the automatic rocker arm machine, and starting the automatic rocker arm machine;
and when the automatic rocker arm machine stops working, standing the solution to be stood for five minutes to obtain the solution to be precipitated.
4. The method for detecting biomolecules according to claim 3, wherein said adjusting the frequency and time of shaking of said automatic rocker arm machine and activating said automatic rocker arm machine comprises:
receiving a shaking program script constructed by a user in advance;
analyzing the shaking program script to obtain the shaking frequency and the shaking time;
and starting the automatic rocker arm machine according to the vibration frequency and the vibration time by utilizing a computer connected with the automatic rocker arm machine.
5. The biomolecule detection method of claim 1, wherein the injecting of the chloroform solution into the solution to be precipitated comprises:
measuring the solution capacity of the solution to be precipitated;
calculating the solution capacity of the chloroform solution according to the pre-constructed proportion and the solution capacity of the solution to be precipitated;
and injecting a chloroform solution into the solution to be precipitated according to the solution capacity of the chloroform solution.
6. The method for detecting biomolecules according to claim 1, wherein the performing the reverse transcription operation on the precipitated solution to obtain a reverse transcription solution comprises:
under the condition of keeping the temperature of the precipitated solution constant, adding a reverse transcription buffer solution, a deoxyribonucleotide mixed solution and a random primer;
after standing for a third time period, adding reverse transcriptase and DEPC water into the precipitated solution to obtain a solution to be reversed;
after allowing the solution to be inverted to stand for a fourth period of time, extracting the reverse transcription solution including cDNA from the solution to be inverted.
7. The biomolecule detection method of claim 1, wherein the preparing of the molecular test paper using the reverse transcription solution and the flowing of the molecular test paper to a test paper transfer path comprise:
loading the reverse transcription solution into an automatic dropper;
and dripping three drops of reverse transcription solution from the reverse transcription solution to test paper by using the automatic dropper to obtain the test paper to be detected by the molecules.
8. A biomolecule detection device, the device further comprising:
the device comprises a lysate injection module, a sample collection module and a sample analysis module, wherein the lysate injection module is used for receiving a concentrated solution to be detected, which is pre-configured by a user, measuring the capacity of the concentrated solution to be detected, and injecting a lysate with a proportion corresponding to the capacity into the concentrated solution to be detected to obtain a solution to be kept static;
a solution separation module, configured to, after shaking the solution to be left standing, stand the solution to be left standing for a first time to obtain a solution to be precipitated, inject a chloroform solution into the solution to be precipitated, and after continuously shaking the solution to be precipitated into which the chloroform solution is injected, stand the solution to be precipitated for a second time to obtain a separation layer solution, where the separation layer solution includes a precipitated solution, separate the precipitated solution from the separation layer solution using ethanol, and perform a reverse transcription operation on the precipitated solution to obtain a reverse transcription solution;
the test paper preparation module is used for preparing molecular test paper to be detected by utilizing the reverse transcription solution, transferring the molecular test paper to be detected to a test paper transmission channel, extracting a proofreading sample liquid by utilizing a sample extraction arm, and filling the proofreading sample liquid into the molecular test paper to be detected in the test paper transmission channel to obtain test paper to be reacted;
and the molecular detection module is used for storing the test paper to be reacted into a greenhouse warehouse, standing whether the test paper to be reacted has a chemical reaction or not, returning to the step of preparing the molecular test paper to be detected if the test paper to be reacted does not have the chemical reaction, and determining the detection result of the concentrated solution to be detected according to the chemical reaction if the test paper to be reacted has the chemical reaction.
9. An electronic device, characterized in that the electronic device comprises:
at least one processor; and the number of the first and second groups,
a memory communicatively coupled to the at least one processor; wherein,
the memory stores instructions executable by the at least one processor to enable the at least one processor to perform the biomolecule detection method of any one of claims 1 to 7.
10. A computer-readable storage medium storing a computer program, wherein the computer program, when executed by a processor, implements the biomolecule detection method according to any one of claims 1 to 7.
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