CN115166153A

CN115166153A - Reagent development experimental method, device, computer equipment and storage medium

Info

Publication number: CN115166153A
Application number: CN202210857305.9A
Authority: CN
Inventors: 王治才; 张毅立; 肖承亮; 骆俊炜; 植立才; 王政
Original assignee: Guangzhou Labsim Biotech Co Ltd
Current assignee: Guangzhou Labsim Biotech Co Ltd
Priority date: 2022-07-20
Filing date: 2022-07-20
Publication date: 2022-10-11

Abstract

The present application relates to an experimental method, apparatus, computer device, storage medium and computer program product for reagent development. The method comprises the following steps: obtaining the type of a reagent for immunoassay, wherein the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent; determining an experimental parameter combination mode according to the type of the reagent for immunoassay; setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence, and automatically carrying out the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished. By adopting the method, different types of reagents for immunoassay can be separately tested according to corresponding test parameter combination modes, which is beneficial to improving the efficiency of reagent development; the experiment is automatically carried out based on the determined experiment parameter combination mode, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

Description

Reagent development experimental method, device, computer equipment and storage medium

Technical Field

The present application relates to the field of reagent development technologies, and in particular, to an experimental method, an experimental apparatus, a computer device, a storage medium, and a computer program product for reagent development.

Background

The existing immune reagent development process needs to arrange and combine raw materials of different manufacturers, various experimental processes can also be arranged and combined, experimental parameters of each process can be adjusted and respectively tested, in addition, a plurality of experimental sample arrangement and combination possibly exist, and the experimental process of the immune reagent development combined in such a way needs to carry out a large amount of manual experiments to select a combination mode of reagents meeting requirements. For example, 4 raw materials are used as a source of a reagent, 4 raw materials are respectively used in each factory, 3 concentration point samples exist in an experiment, 4 process combinations exist in the experiment, 4 substrate types exist in the experiment, 4 magnetic separation types exist in the experiment, and 2 types exist in the experiment sample, and the formula is calculated according to the experiment times: the experiment times = manufacturer times, raw material times, process combination times, magnetic separation types, concentration points, and sample types, and the number of manual experiments is calculated to be 4 times 2 times 3 times=, 6144 times, and the experiment method for developing the immunological reagent needs a large number of manual experiments to select the combination mode of the individual reagents meeting the requirements.

Therefore, the existing experimental method for reagent development needs to manually complete a large number of experimental combinations, is time-consuming and labor-consuming, and may bring more influence of artificial uncertainty factors, so that the problem of low reagent development efficiency exists.

Disclosure of Invention

In view of the above, it is necessary to provide an experimental method, an experimental apparatus, a computer device, a computer-readable storage medium, and a computer program product for reagent development, which can improve the efficiency of reagent development, in order to solve the problem of low reagent development efficiency in the conventional experimental method for reagent development.

In a first aspect, the present application provides an experimental method for reagent development. The method comprises the following steps:

obtaining the type of a reagent for immunoassay, wherein the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent;

determining an experimental parameter combination mode according to the type of the reagent for immunoassay;

setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence, and automatically carrying out the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

In one embodiment, the determination of the combination of experimental parameters based on the type of the immunoassay reagent comprises:

if the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the chemiluminescence method immunodetection reagent;

and if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, determining an experiment parameter combination mode according to the experiment parameters corresponding to the fluorescence immunoassay reagent.

In one embodiment, determining the combination of the experimental parameters according to the experimental parameters corresponding to the chemiluminescent immunoassay reagent comprises:

optionally selecting at least one experimental parameter from experimental parameters corresponding to the chemiluminescence immunoassay reagent;

and taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

In one embodiment, determining the combination of the experimental parameters according to the experimental parameters corresponding to the fluorescence immunoassay reagent comprises:

optionally selecting at least one experimental parameter from parameters corresponding to the fluorescence immunoassay reagent;

if the type of the reagent for immunoassay is a chemiluminescent immunoassay reagent, the chemiluminescent immunoassay reagent corresponds to at least one raw material, and each raw material in the at least one raw material comprises at least one dosage, setting the corresponding dosage for each raw material;

and taking the combination mode formed by the dosages corresponding to the raw materials as the experimental parameter combination mode.

In one embodiment, each of the assay parameters for the chemiluminescent immunoassay reagent comprises at least one numerical value;

the method for combining the experimental parameters comprises the following steps:

for each of the at least one experimental parameter, determining a corresponding numerical value;

and taking a combination mode formed by numerical values corresponding to at least one experimental parameter as an experimental parameter combination mode.

In one embodiment, the setting of the experiment order of the experiment parameter combination mode includes:

arranging at least one numerical value in each experimental parameter, and setting a numerical value arrangement sequence corresponding to each experimental parameter;

and setting the experiment sequence of the experiment parameter combination mode according to the numerical value arrangement sequence corresponding to each experiment parameter.

In a second aspect, the present application also provides an experimental apparatus for reagent development. The device comprises:

the acquisition module is used for acquiring the type of the reagent for immunoassay, wherein the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent;

the determination module is used for determining an experiment parameter combination mode according to the type of the reagent for the immunodetection;

the experiment module is used for setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence and automatically carrying out the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

In a third aspect, the present application also provides a computer device. The computer device comprises a memory and a processor, the memory stores a computer program, and the processor realizes the following steps when executing the computer program:

In a fourth aspect, the present application further provides a computer-readable storage medium. A computer-readable storage medium, on which a computer program is stored, which computer program, when being executed by a processor, carries out the steps of:

In a fifth aspect, the present application further provides a computer program product. Computer program product comprising a computer program which when executed by a processor performs the steps of:

According to the experimental method, the experimental device, the computer equipment, the storage medium and the computer program product for reagent development, the type of the reagent for immunoassay is obtained and comprises the chemiluminescence immunoassay reagent or the fluorescence immunoassay reagent, and the method for determining the experimental parameter combination mode is determined according to different types of the reagent for immunoassay, and the reagents for immunoassay of different types can be separately tested according to the corresponding experimental parameter combination modes, so that the efficiency of reagent development is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

Drawings

FIG. 1 is a diagram of an environment in which an experimental method for reagent development is used in one embodiment;

FIG. 2 is a schematic flow diagram of an experimental method for reagent development in one embodiment;

FIG. 3 is a schematic flow chart of an experimental method for reagent development in another embodiment;

FIG. 4 is a schematic diagram illustrating a sub-flow of S420 according to an embodiment;

FIG. 5 is a schematic sub-flow chart of S440 in one embodiment;

FIG. 6 is a schematic flowchart of an experimental method for reagent development in yet another embodiment;

FIG. 7 is a schematic diagram illustrating a sub-flow of S424 in one embodiment;

FIG. 8 is a schematic flow chart of an experimental method for reagent development in a further embodiment;

FIG. 9 is a block diagram showing the structure of an experimental apparatus for reagent development in one embodiment;

fig. 10 is an internal structural diagram of a computer device in one embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

The experimental method for reagent development provided by the embodiment of the application can be applied to the application environment shown in fig. 1. Wherein, the terminal 102 is connected with and in data communication with the reagent development experiment platform 104. The data storage system may store data that the terminal 102 needs to process. The data storage system may be integrated on the terminal 102 or may reside on another server. The terminal 102 acquires the type of the reagent for immunoassay, wherein the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent; determining an experimental parameter combination mode according to the type of the reagent for immunoassay; setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence, and automatically carrying out the experiment according to the experiment sequence and the reagent development experiment platform 104; and outputting the experimental result of the reagent for immunoassay after the experiment is finished. The terminal 102 may be, but not limited to, various personal computers, notebook computers, smart phones, tablet computers, internet of things devices and portable wearable devices, and the internet of things devices may be smart speakers, smart televisions, smart air conditioners, smart car-mounted devices, and the like. The portable wearable device can be a smart watch, a smart bracelet, a head-mounted device, and the like.

In one embodiment, as shown in fig. 2, an experimental method for reagent development is provided, which is illustrated by applying the method to the terminal 102 in fig. 1, and includes the following steps:

s200, obtaining the type of the reagent for immunoassay, wherein the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent.

The immunoassay reagent is a reagent for detecting some disease-related markers (antibodies or antigens) in samples such as blood, saliva, urine, etc. of human or animals, which is prepared by labeling antigens or antibodies using some chemical techniques, and is a reagent for immunoassay because these reagents are based on the immunoreaction of antigen-antibodies. The type of the reagent for immunoassay includes a chemiluminescent immunoassay reagent or a fluorescent immunoassay reagent. The chemiluminescence immunoassay method is an analysis method combining chemiluminescence and immunoreaction, and comprises an immunochemical reaction part and a chemiluminescence reaction part, wherein the immunochemical reaction part is used for marking chemiluminescence or enzyme on an antigen or an antibody and forming an antigen-antibody immune complex through antigen or antibody specific reaction, the chemiluminescence reaction part is used for adding an oxidant and an alkaline substance (namely an acridine lipid method) or a luminous substrate of enzyme (namely an enzymatic method) after the immunoreaction is finished, the chemiluminescence reaction part emits photons to release energy to return to a stable ground state after being oxidized by the oxidant or catalyzed by the enzyme in an alkaline environment, the luminous intensity can be detected by using a luminous signal measuring instrument, and the chemiluminescence immunoassay reagent realizes the purpose of detecting the concentration of a substance to be detected according to the relation between the luminous intensity and the concentration of the substance to be detected. The fluorescence immunoassay reagent is a reagent for immunoassay by adopting a fluorescence immunoassay technology, and the fluorescence immunoassay technology takes a specific antibody or antigen marked by a fluorescent substance as a standard reagent and is mainly used for analysis, identification and quantitative determination of the corresponding antigen or antibody.

And S400, determining an experimental parameter combination mode according to the type of the reagent for immunoassay.

The combination mode of the experimental parameters refers to at least one parameter combination obtained by arranging and combining at least one parameter involved in the experimental process of reagent development in a certain mode, and the combination mode of the experimental parameters comprises an experimental parameter combination mode corresponding to a chemiluminescence immunoassay reagent or an experimental parameter combination mode corresponding to a fluorescence immunoassay reagent. Because the chemiluminescence immunoassay reagent and the fluorescence immunoassay reagent have different detection methods and involve different experimental parameters, in the development process of the immunoassay reagent, the experimental parameter combination mode corresponding to the chemiluminescence immunoassay reagent and the experimental parameter combination mode corresponding to the fluorescence immunoassay reagent need to be determined according to the type of the immunoassay reagent, and the experimental parameter combination mode corresponding to the type of the immunoassay reagent is determined according to the types of different immunoassay reagents.

S600, setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence, and automatically performing the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

Wherein, different immunoassay reagent types are different in detection method, different experiment orders are set for different immunoassay reagent types, and reagent development experiments are carried out according to the experiment orders corresponding to the different immunoassay reagent types. The experiment sequence of the experiment parameter combination mode comprises an experiment sequence of an experiment parameter combination mode corresponding to the chemiluminescence immunoassay reagent or an experiment sequence of an experiment parameter combination mode corresponding to the fluorescence immunoassay reagent, and the experiment sequence of the experiment parameter combination mode comprises the following steps: selecting one or more of a platform period test, a project experiment, a project evaluation, a performance evaluation and a stability test, determining an experiment type, and setting an experiment sequence of the experiment type; selecting one or more of a raw material combination mode, a flow combination mode and an experiment parameter combination mode from the corresponding experiment types, determining an experiment combination mode, and setting an experiment sequence of the experiment combination mode; and determining the combination mode of the dose constitution corresponding to the raw materials or the combination mode of the numerical value constitution corresponding to the experimental parameters under the corresponding experimental combination mode, and setting the experimental sequence of the experimental parameter combination mode according to the combination mode of the dose constitution corresponding to the raw materials or the combination mode of the numerical value constitution corresponding to the experimental parameters. Starting the experiment of reagent development according to the experiment sequence, and automatically carrying out the experiment of reagent development according to the experiment sequence; and outputting an experimental result of the reagent for immunoassay after the experiment for reagent development is finished, wherein the experimental result of the reagent for immunoassay comprises an experimental result corresponding to a chemiluminescence immunoassay reagent or an experimental result corresponding to a fluorescence immunoassay reagent.

In the experiment method for reagent development, the type of the reagent for immunoassay is obtained, the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent, and the method for the experiment parameter combination mode is determined according to different types of the reagents for immunoassay, so that the reagents for immunoassay of different types can be separately tested according to the corresponding experiment parameter combination modes, and the efficiency of reagent development is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, as shown in fig. 3, the method for determining the combination of experimental parameters according to the type of the reagent for immunoassay includes:

s420, if the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the chemiluminescence method immunodetection reagent;

s440, if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the fluorescence immunoassay reagent.

In this embodiment, the type of the reagent for immunoassay is a chemiluminescent immunoassay reagent or a fluorescent immunoassay reagent, and the reagent development involves different experimental parameters, for example, the experimental parameters corresponding to the chemiluminescent immunoassay reagent include the dosage of raw materials and the parameters of each experimental process, the experimental parameters corresponding to the fluorescent immunoassay reagent include the number of samples and the types of the samples, and any one or more than one experimental parameters are combined together to obtain a set of experimental parameter combination methods. If the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, selecting experimental parameters from the experimental parameters corresponding to the chemiluminescence method immunodetection reagent for permutation and combination to obtain an experimental parameter combination mode corresponding to the chemiluminescence method immunodetection reagent, and taking the experimental parameter combination mode corresponding to the chemiluminescence method immunodetection reagent as an experimental parameter combination mode, wherein the experimental parameter combination mode comprises at least one combination mode; if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, selecting experimental parameters from the experimental parameters corresponding to the fluorescence immunoassay reagent for permutation and combination to obtain an experimental parameter combination mode corresponding to the fluorescence immunoassay reagent, and taking the experimental parameter combination mode corresponding to the fluorescence immunoassay reagent as an experimental parameter combination mode, wherein the experimental parameter combination mode comprises at least one combination mode.

In the scheme of the embodiment, under the condition that the type of the reagent for immunoassay is the chemiluminescence immunoassay reagent, the combination mode of the experimental parameters is determined according to the experimental parameters corresponding to the chemiluminescence immunoassay reagent; under the condition that the type of the reagent for immunoassay is the fluorescence immunoassay reagent, the experimental parameter combination mode is determined according to the experimental parameters corresponding to the fluorescence immunoassay reagent, and the method for determining the experimental parameter combination mode according to the types and the corresponding experimental parameters of different reagents for immunoassay is characterized in that the reagents for immunoassay of different types can be separately tested according to the corresponding experimental parameter combination modes, so that the reagent development efficiency is improved.

In one embodiment, as shown in fig. 4, determining the combination of the experiment parameters according to the experiment parameters corresponding to the chemiluminescent immunoassay reagent comprises:

s422, selecting at least one experiment parameter from experiment parameters corresponding to the chemiluminescence immunoassay reagent;

and S424, taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

In this embodiment, the experimental parameters corresponding to the chemiluminescent immunoassay reagent include: the method comprises the following steps of (1) sample liquid amount, diluent liquid amount, reagent parameters, dilution and uniform mixing times, sample shaking times, sample incubation time, single magnetic separation times, continuous magnetic separation times, substrate shaking times, substrate incubation time, first enzymatic test parameters, second enzymatic test parameters and direct luminescence test parameters; the sample liquid volume is the sample liquid volume added into the reaction cup, the diluent liquid volume is the diluent liquid volume added into the reaction cup, the reagent parameters comprise the name of the reagent added into the reaction cup, the corresponding bottle number and the reagent liquid volume, the number of dilution and uniform mixing is the number of dilution and uniform mixing by using a uniform mixing structure, the number of sample shaking is the number of sample shaking by using a shaking structure, the sample incubation time is the sample incubation time by using an incubation disc, the number of single magnetic separation is the number of magnetic separation by using a single magnetic separation assembly, the number of continuous magnetic separation is the number of magnetic separation by using the magnetic separation disc, the number of substrate shaking is the number of shaking by using the shaking structure, the substrate incubation time is the substrate incubation time by using the incubation disc, the first enzymatic test parameter comprises the substrate type, the liquid volume and the enzymatic test time when the experiment is carried out by using the first enzymatic method, the second enzymatic test parameter comprises the substrate type, the substrate liquid volume and the test time when the experiment is carried out by using the second enzymatic method, and the direct luminescence test parameter comprises the substrate type, the substrate liquid volume and the test time when the direct luminescence test method is used. At least one experiment parameter is selected from experiment parameters corresponding to the chemiluminescence immunoassay reagent, specifically, a plurality of combination modes are formed by selecting the experiment parameters from the experiment parameters corresponding to the chemiluminescence immunoassay reagent for a plurality of times, and the plurality of combination modes are used as experiment parameter combination modes, wherein at least one experiment parameter selected each time forms one combination mode, and the combination is used as one experiment parameter combination mode. For example, two optional experimental parameters are selected from the experimental parameters corresponding to the chemiluminescence immunoassay reagent for the first time: the amount of the diluent and the reagent parameters are selected, and four experimental parameters are selected from the experimental parameters corresponding to the chemiluminescence immunoassay reagent for the second time: the sample shaking times, the sample incubation time, the single magnetic separation times and the continuous magnetic separation times are determined, and the experimental parameter combination mode comprises two combination modes, wherein the first combination mode is as follows: the second combination mode is as follows: the combination mode of the sample shaking times, the sample incubation time, the single magnetic separation times and the continuous magnetic separation times.

In the scheme of the embodiment, at least one experiment parameter is selected from experiment parameters corresponding to the immunoassay reagent by the chemiluminescence method, and a combination mode formed by the at least one experiment parameter is used as a method for combining the experiment parameters, so that different types of reagents for immunoassay can be separately tested according to the corresponding experiment parameter combination modes, and the development efficiency of the reagents is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, as shown in fig. 5, determining the combination of the experiment parameters according to the experiment parameters corresponding to the fluorescence immunoassay reagent comprises:

s442, selecting at least one experiment parameter from experiment parameters corresponding to the fluorescence immunoassay reagent;

and S444, taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

In this embodiment, the experimental parameters corresponding to the fluorescence immunoassay reagent include: sample number, sample type, sample amount, diluent formula, diluent liquid amount, mixed liquid amount, sample adding amount, mixing frequency, incubation temperature, pre-reading time, incubation time and sample needle cleaning frequency. The method comprises the steps of obtaining a sample, a diluent, a mixing liquid, a sample needle and a sample needle, wherein the sample quantity is the quantity of the sample for carrying out a fluorescence immunoassay reagent development experiment, the sample type is the type of the sample for carrying out the fluorescence immunoassay reagent development experiment, the sampling quantity is the quantity of a selected sample, the diluent formula is the formula of the diluent for carrying out the fluorescence immunoassay reagent development experiment, the diluent taking liquid quantity is the liquid quantity of the selected diluent, the mixing liquid quantity is the liquid quantity after the sample and the diluent are mixed, the sample adding quantity is the quantity of a newly added sample, the mixing frequency is the frequency of mixing operation of the sample and the diluent, the incubation temperature is the temperature for incubation in an incubation tray, the pre-reading time is the time for pre-reading, the incubation time is the time for incubating in the incubation tray, and the cleaning frequency of the sample needle is the frequency for cleaning the sample needle. At least one experiment parameter is selected from experiment parameters corresponding to the fluorescence immunoassay reagent, specifically, a plurality of combination modes are formed by selecting the experiment parameters from the experiment parameters corresponding to the fluorescence immunoassay reagent for a plurality of times, the plurality of combination modes are used as experiment parameter combination modes, wherein at least one experiment parameter selected at each time forms one combination mode, and the combination is used as one experiment parameter combination mode. For example, two optional experimental parameters are selected from the experimental parameters corresponding to the fluorescence immunoassay reagent for the first time: and selecting four experimental parameters from the experimental parameters corresponding to the fluorescence immunoassay reagent for the second time according to the number and the type of the samples: determining the combination modes of the experimental parameters including two combination modes according to the number of samples, the types of the samples, the sampling amount and the formula of the diluent, wherein the first combination mode is as follows: the second combination mode is as follows: sample quantity, sample type, sampling amount and diluent formula combination mode.

In the scheme of the embodiment, at least one experiment parameter is selected from experiment parameters corresponding to the immunoassay reagent by a fluorescence method, and a combination mode formed by the at least one experiment parameter is used as a method for combining the experiment parameters, so that different types of reagents for immunoassay can be separately tested according to the corresponding experiment parameter combination modes, and the development efficiency of the reagents is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, as shown in fig. 6, the determination of the combination of experimental parameters according to the type of the reagent for immunoassay includes:

s462, if the type of the reagent for immunoassay is a chemiluminescent immunoassay reagent, the chemiluminescent immunoassay reagent corresponds to at least one raw material, and each raw material of the at least one raw material includes at least one dose, setting a corresponding dose for each raw material;

and S464, taking a combination mode formed by the dosages corresponding to the at least one raw material as an experimental parameter combination mode.

In this embodiment, in the reagent development, at least one material is often required to perform an experiment of reagent development, different types of reagents for immunoassay correspond to different materials, and the material corresponding to the type of the reagent for immunoassay can be specified according to the type of the reagent for immunoassay. The dosage refers to the amount of the raw material, and may be the amount of liquid of the raw material. In the case where the type of the reagent for immunoassay is a chemiluminescent immunoassay reagent, if the raw material of the chemiluminescent immunoassay reagent includes at least one type and each of the at least one raw material includes at least one dose, the dose corresponding to each raw material is set for each raw material, a combination of the doses corresponding to each of the at least one raw material is used as an experimental parameter combination, specifically, the dose corresponding to each raw material is divided into a plurality of optional doses, the doses obtained in a plurality of times form a plurality of combinations, and the plurality of combinations are used as an experimental parameter combination, wherein the dose corresponding to each optional raw material in each raw material forms one combination. For example, the raw materials of the chemiluminescent immunoassay reagent include R1 and R2, the dosage corresponding to R1 includes 3 ml and 4 ml, and the dosage corresponding to R2 includes 10 ml and 11 ml, and the experimental parameter combination includes four combinations, where the first combination is: taking 3 ml of R1 and 10 ml of R2; the second combination mode is as follows: taking 4 ml of R1 and 10 ml of R2; the third combination mode is as follows: taking 3 ml of R1 and 11 ml of R2; the fourth combination mode is as follows: r1 is 4 ml, and R2 is 11 ml.

In the embodiment, when the type of the reagent for immunoassay is the chemiluminescent immunoassay reagent and the chemiluminescent immunoassay reagent corresponds to at least one raw material, and each raw material in the at least one raw material comprises at least one dose, the dose corresponding to each raw material is set for each raw material, and a combination mode formed by the doses corresponding to the at least one raw material is used as a method for combining experimental parameters, so that the reagents for immunoassay of different types can be separately tested according to the corresponding experimental parameter combination mode, and the reagent development efficiency is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, as shown in FIG. 7, each of the assay parameters for the chemiluminescent immunoassay reagent comprises at least one numerical value; the combination mode formed by at least one experimental parameter is used as the experimental parameter combination mode, and the combination mode comprises the following steps:

s425, determining a corresponding numerical value for each experimental parameter of at least one experimental parameter;

s426, a combination mode formed by the numerical values corresponding to the at least one experimental parameter is used as an experimental parameter combination mode.

In this embodiment, each of the experiment parameters corresponding to the chemiluminescent immunoassay reagent often includes at least one value, for example, the sample incubation time includes, but is not limited to, 5 minutes, 10 minutes or 15 minutes, for each of the at least one experiment parameter, the value corresponding to each experiment parameter is determined, the combination of the values corresponding to each of the at least one experiment parameter is used as the experiment parameter combination, specifically, the value corresponding to each of the at least one experiment parameter is divided into a plurality of optional values, the values obtained in the plurality of optional values form a plurality of combinations, and the plurality of combinations of experiment parameters are used as the experiment parameter combination, wherein the value corresponding to each optional experiment parameter in each experiment parameter forms one combination. For example, the experiment parameters corresponding to the chemiluminescent immunoassay reagent include sample liquid amount and sample shaking times, the values corresponding to the sample liquid amount include 20 ml and 30 ml, and the values corresponding to the sample shaking times include 3 times and 5 times, so that the experiment parameter combination mode includes four combination modes, wherein the first combination mode is: the sample liquid amount is 20 ml and the sample shaking frequency is 3 times, and the second combination mode is as follows: the liquid amount of the sample is 20 milliliters, the shaking times of the sample are 5 times, and the third combination mode is as follows: the sample liquid amount is 30 ml and the sample shaking frequency is 3 times, and the fourth combination mode is as follows: the amount of the sample solution was 30 ml and the number of shaking-up times of the sample was 5 times.

In the scheme of the embodiment, by determining the corresponding numerical value for each experiment parameter of at least one experiment parameter corresponding to the immunoassay reagent by the chemiluminescence method, and using a combination mode formed by the numerical values corresponding to the at least one experiment parameter as a method of the experiment parameter combination mode, reagents for immunoassay of different types can be separately tested according to the corresponding experiment parameter combination mode, thereby being beneficial to improving the reagent development efficiency; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, each of the assay parameters for the fluorescent immunoassay reagent comprises at least one value; the combination mode formed by at least one experimental parameter is used as the experimental parameter combination mode, and the combination mode comprises the following steps: for each of the at least one experimental parameter, determining a corresponding numerical value; and taking a combination mode formed by numerical values corresponding to at least one experimental parameter as an experimental parameter combination mode.

In this embodiment, each of the experiment parameters corresponding to the fluorescence immunoassay reagent often includes at least one value, for example, the number of samples includes but is not limited to 100, 150 or 200, for each of the at least one experiment parameter, the value corresponding to each experiment parameter is determined, the combination of the values corresponding to the at least one experiment parameter is used as the experiment parameter combination, specifically, the value obtained from the at least one experiment parameter is divided into a plurality of optional values, the values obtained from the plurality of optional values form a plurality of combinations, and the plurality of combinations of experiment parameters are combined, wherein the value corresponding to each optional experiment parameter in each experiment parameter forms one combination. For example, the experiment parameters corresponding to the fluorescence immunoassay reagent include the number of times of washing the sample needle and the number of samples, the values corresponding to the number of times of washing the sample needle include 3 times and 5 times, and the values corresponding to the number of samples include 100 times and 200 times, and the experiment parameter combination mode includes four combination modes, wherein the first combination mode is: the number of times of cleaning the sample needle is 3 and the number of samples is 100, and the second combination mode is as follows: the cleaning times of the sample needles are 3 times, the number of the samples is 200, and the third combination mode is as follows: the number of times of cleaning the sample needle is 4 and the number of samples is 100, and the fourth combination mode is as follows: the number of sample needle washes was 4 and the number of samples was 200.

In the scheme of the embodiment, by determining the corresponding numerical value for each experiment parameter of at least one experiment parameter corresponding to the fluorescence immunoassay reagent, and using a combination mode formed by the numerical values corresponding to the at least one experiment parameter as a method for combining the experiment parameters, different types of reagents for immunoassay can be separately tested according to the corresponding combination modes of the experiment parameters, which is beneficial to improving the development efficiency of the reagents; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, as shown in fig. 8, the setting of the experiment sequence of the experiment parameter combination manner includes:

s620, arranging at least one numerical value in each experimental parameter, and setting a numerical value arrangement sequence corresponding to each experimental parameter;

and S640, setting the experiment sequence of the experiment parameter combination mode according to the numerical value arrangement sequence corresponding to each experiment parameter.

In this embodiment, at least one numerical value in each experimental parameter in the experimental parameters corresponding to the chemiluminescent immunoassay reagent is arranged, and the numerical value arrangement order corresponding to each experimental parameter is set, where the arrangement method may be full arrangement of at least one numerical value in each experimental parameter, or may be that a preset number of numerical values are taken out from at least one numerical value in each experimental parameter, the taken out preset number of numerical values are arranged, and the numerical value arrangement order corresponding to each experimental parameter is set. And arranging each experimental parameter in the experimental parameters corresponding to the chemiluminescent immunoassay reagent according to the corresponding numerical value arrangement sequence to obtain the experimental sequence corresponding to the chemiluminescent immunoassay reagent, and taking the experimental sequence corresponding to the chemiluminescent immunoassay reagent as the experimental sequence corresponding to the experimental parameter combination mode.

According to the method, at least one numerical value in each experimental parameter in the experimental parameters corresponding to the chemiluminescence immunoassay reagent is arranged, the numerical value arrangement sequence corresponding to each experimental parameter is set, the experimental sequence of the experimental parameter combination mode is further set, and the experiment is automatically carried out according to the set experimental sequence based on the determined experimental parameter combination mode, so that errors caused by manual experiment of a large number of experimental parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In another embodiment, the setting of the experiment sequence of the experiment parameter combination mode includes: arranging at least one numerical value in each experimental parameter corresponding to the fluorescence immunoassay reagent, setting a numerical value arrangement sequence corresponding to each experimental parameter, and setting an experimental sequence of an experimental parameter combination mode according to the numerical value arrangement sequence corresponding to each experimental parameter.

In this embodiment, at least one numerical value in each of the experimental parameters corresponding to the fluorescence immunoassay reagent is arranged, and a numerical value arrangement order corresponding to each of the experimental parameters is set, where the arrangement method may be a full arrangement of at least one numerical value in each of the experimental parameters, or may be a method in which a preset number of numerical values are taken out from at least one numerical value in each of the experimental parameters, the taken out preset number of numerical values are arranged, and a numerical value arrangement order corresponding to each of the experimental parameters is set. And arranging each experimental parameter in the experimental parameters corresponding to the fluorescence immunoassay reagent according to the corresponding numerical value arrangement sequence to obtain the experimental sequence corresponding to the fluorescence immunoassay reagent, and taking the experimental sequence corresponding to the fluorescence immunoassay reagent as the experimental sequence corresponding to the experimental parameter combination mode.

According to the method of the embodiment, at least one numerical value in each experimental parameter in the experimental parameters corresponding to the fluorescence immunoassay reagent is arranged, the numerical value arrangement sequence corresponding to each experimental parameter is set, the experimental sequence of the experimental parameter combination mode is further set, and the experiment is automatically carried out according to the set experimental sequence based on the determined experimental parameter combination mode, so that errors caused by manual experiment corresponding to a large number of experimental parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

To illustrate the experimental methods and effects of reagent development in this embodiment, a most detailed embodiment is described below, wherein the types of reagents for immunoassay include chemiluminescent immunoassay reagents or fluorescent immunoassay reagents:

obtaining the type of a reagent for immunoassay;

if the type of the reagent for immunoassay is a chemiluminescence immunoassay reagent, setting corresponding doses for each raw material, and taking a combination mode formed by the doses corresponding to at least one raw material as an experimental parameter combination mode, wherein the first combination mode is a reagent component M of 50 microliters, a reagent component R1 of 50 microliters, a reagent component R2 of 50 microliters and a reagent component R3 of 50 microliters; the second combination mode is two corresponding experiment sequences of a reagent component M of 50 microliter, a reagent component R1 of 100 microliter, a reagent component R2 of 50 microliter and a reagent component R3 of 50 microliter, and the experiment parameter combination modes are carried out in sequence, wherein the first experiment sequence is that the reagent component M of 50 microliter, the reagent component R1 of 50 microliter, the reagent component R2 of 50 microliter and the reagent component R3 of 50 microliter, the second experiment sequence is that the reagent component M of 50 microliter, the reagent component R1 of 100 microliter, the reagent component R2 of 50 microliter and the reagent component R3 of 50 microliter are carried out in sequence, the experiment is started according to the experiment sequence and automatically carried out, and the experiment result of the reagent for the immunodetection is output after the experiment is finished;

if the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, selecting the following experimental parameters from the corresponding chemiluminescence method immunodetection reagent: the method comprises the following steps of sequentially carrying out the steps of sample liquid volume of 50 microliter, dilution liquid volume of 150 microliter, reagent component M of 50 microliter, reagent component R1 of 100 microliter, reagent component R2 of 50 microliter, reagent component R3 of 50 microliter, dilution mixing times of 3 times, sample incubation time of 5 minutes, single magnetic separation of 3 times, substrate of a first enzymatic test parameter of 200 microliter, shaking times of 3 seconds and substrate incubation time of 5 minutes, wherein as an experimental parameter combination mode, the corresponding experimental sequence of the experimental parameter combination mode is that the sample liquid volume is 50 microliter, the dilution liquid volume is 150 microliter, reagent component M of 50 microliter, reagent component R1 of 100 microliter, reagent component R2 of 50 microliter, reagent component R3 of 50 microliter, dilution mixing times of 3 times, sample incubation time of 5 minutes, single magnetic separation of 3 times, substrate of a first enzymatic test parameter of 200 microliter, shaking times of substrate of 3 seconds and substrate incubation time of 5 minutes, and automatically outputting an experimental reagent for immune detection after the experiment is finished;

if the type of the reagent for immunoassay is a fluorescence immunoassay reagent, selecting the following parameters from the parameters corresponding to the fluorescence immunoassay reagent: the number of samples is 200, the types of the samples are antigens, the sampling amount is 50 ml, the formula of the diluent is formula one, the liquid taking amount of the diluent is 100 ml, the liquid mixing amount is 80 ml, the sample adding amount is 20 ml, the mixing frequency is 5 times, the incubation temperature is 38 ℃, the pre-reading time is 3 seconds, the incubation time is 5 minutes and the cleaning frequency of a sample needle is 4 times, as an experiment parameter combination mode, the experiment sequence corresponding to the experiment parameter combination mode is as follows: the method comprises the following steps of sequentially carrying out 200 samples, antigen samples, 50 ml samples, a formula I of diluent, 100 ml diluent liquid, 80 ml mixed liquid, 20 ml sample adding, 5 times of mixing, 38 ℃ incubation temperature, 3 seconds of pre-reading time, 5 minutes of incubation time and 4 times of sample needle cleaning, starting an experiment according to an experiment sequence, automatically carrying out the experiment, and outputting an experiment result of the reagent for the immunodetection after the experiment is finished.

According to the experimental method for reagent development, the types of the reagents for immunoassay are obtained, the types of the reagents for immunoassay comprise chemiluminescence immunoassay reagents or fluorescence immunoassay reagents, and the experimental parameter combination mode is determined according to different types of the reagents for immunoassay, so that the reagents for immunoassay of different types can be separately tested according to the corresponding experimental parameter combination modes, and the efficiency of reagent development is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

It should be understood that, although the steps in the flowcharts related to the embodiments described above are shown in sequence as indicated by the arrows, the steps are not necessarily performed in sequence as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least a part of the steps in the flowcharts related to the embodiments described above may include multiple steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, and the execution order of the steps or stages is not necessarily sequential, but may be rotated or alternated with other steps or at least a part of the steps or stages in other steps.

Based on the same inventive concept, the embodiment of the present application further provides a reagent development experimental apparatus for implementing the above-mentioned reagent development experimental method. The solution to the problem provided by the device is similar to the solution described in the above method, so the specific limitations in one or more embodiments of the experimental device for reagent development provided below can be referred to the limitations in the above experimental method for reagent development, and are not described herein again.

In one embodiment, as shown in FIG. 9, there is provided a reagent development assay device 100 comprising: an acquisition module 120, a determination module 140, and an experiment module 160, wherein:

the obtaining module 120 is configured to obtain a type of the reagent for immunoassay, where the type of the reagent for immunoassay includes a chemiluminescent immunoassay reagent or a fluorescent immunoassay reagent.

And the determining module 140 is used for determining the experimental parameter combination mode according to the type of the reagent for immunoassay.

The experiment module 160 is used for setting an experiment sequence of an experiment parameter combination mode, starting an experiment according to the experiment sequence and automatically performing the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

According to the experimental device for reagent development, the types of the reagents for immunoassay are obtained, the types of the reagents for immunoassay comprise chemiluminescence immunoassay reagents or fluorescence immunoassay reagents, and the method for determining the experimental parameter combination mode is performed according to different types of the reagents for immunoassay, so that the reagents for immunoassay of different types can be separately tested according to the corresponding experimental parameter combination modes, and the efficiency of reagent development is improved; based on the determined experiment parameter combination mode, the experiment is automatically carried out according to the set experiment sequence, so that errors caused by manual experiment corresponding to a large number of experiment parameter combination modes can be avoided, and meanwhile, the reagent development efficiency is further improved.

In one embodiment, in determining the combination of experimental parameters according to the type of the reagent for immunoassay, the determining module 140 is further configured to:

In one embodiment, the determination module 140 is further configured to:

In one embodiment, the determining module 140 is further configured to, in determining the combination of the experiment parameters according to the experiment parameters corresponding to the fluorescence immunoassay reagent:

optionally selecting at least one experimental parameter from the parameters corresponding to the fluorescence immunoassay reagent;

In one embodiment, in determining the combination of experimental parameters according to the type of the reagent for immunoassay, the determination module 140 is further configured to:

and taking the combination mode formed by the dosages corresponding to the at least one raw material as an experimental parameter combination mode.

In one embodiment, each of the assay parameters for the chemiluminescent immunoassay reagent comprises at least one numerical value; the determining module 140 is further configured to, as an aspect of the combination of the experiment parameters, a combination of at least one experiment parameter:

In one embodiment, in setting the experiment order of the experiment parameter combination, the experiment module 160 is further configured to:

The modules in the experimental device for reagent development can be wholly or partially realized by software, hardware and a combination thereof. The modules can be embedded in a hardware form or independent of a processor in the computer device, and can also be stored in a memory in the computer device in a software form, so that the processor can call and execute operations corresponding to the modules.

In one embodiment, a computer device is provided, which may be a server, and the internal structure thereof may be as shown in fig. 10. The computer device includes a processor, a memory, an Input/Output interface (I/O for short), and a communication interface. The processor, the memory and the input/output interface are connected through a system bus, and the communication interface is connected to the system bus through the input/output interface. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The database of the computer equipment is used for storing the type of the reagent for immunoassay, the combination mode of the experimental parameters, the experimental result of the reagent for immunoassay, the experimental parameters corresponding to the reagent for immunoassay by a fluorescence method, the experimental parameters corresponding to the reagent for immunoassay by a chemiluminescence method, the numerical values corresponding to the experimental parameters and the dosage corresponding to the raw materials. The input/output interface of the computer device is used for exchanging information between the processor and an external device. The communication interface of the computer device is used for connecting and communicating with an external terminal through a network. The computer program is executed by a processor to implement an experimental method of reagent development.

Those skilled in the art will appreciate that the architecture shown in fig. 10 is merely a block diagram of some of the structures associated with the disclosed aspects and is not intended to limit the computing devices to which the disclosed aspects apply, as particular computing devices may include more or less components than those shown, or may combine certain components, or have a different arrangement of components.

In one embodiment, a computer device is provided, comprising a memory and a processor, the memory having a computer program stored therein, the processor implementing the following steps when executing the computer program:

In one embodiment, the processor, when executing the computer program, further performs the steps of:

if the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the chemiluminescence method immunodetection reagent; if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the fluorescence immunoassay reagent.

optionally selecting at least one experimental parameter from experimental parameters corresponding to the chemiluminescence immunoassay reagent; and taking a combination mode formed by at least one experimental parameter as an experimental parameter combination mode.

optionally selecting at least one experimental parameter from parameters corresponding to the fluorescence immunoassay reagent; and taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

if the type of the reagent for immunoassay is a chemiluminescent immunoassay reagent, the chemiluminescent immunoassay reagent corresponds to at least one raw material, and each raw material in the at least one raw material comprises at least one dosage, setting the corresponding dosage for each raw material; and taking the combination mode formed by the dosages corresponding to the at least one raw material as an experimental parameter combination mode.

each experimental parameter in the experimental parameters corresponding to the chemiluminescence immunoassay reagent comprises at least one numerical value; for each of the at least one experimental parameter, determining a corresponding numerical value; and taking a combination mode formed by numerical values corresponding to at least one experimental parameter as an experimental parameter combination mode.

arranging at least one numerical value in each experimental parameter, and setting a numerical value arrangement sequence corresponding to each experimental parameter; and setting the experiment sequence of the experiment parameter combination mode according to the numerical value arrangement sequence corresponding to each experiment parameter.

In one embodiment, a computer-readable storage medium is provided, having a computer program stored thereon, which when executed by a processor, performs the steps of:

In one embodiment, the computer program when executed by the processor further performs the steps of:

if the type of the reagent for the immunodetection is the chemiluminescence method immunodetection reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the chemiluminescence method immunodetection reagent; and if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, determining an experiment parameter combination mode according to the experiment parameters corresponding to the fluorescence immunoassay reagent.

optionally selecting at least one experimental parameter from experimental parameters corresponding to the chemiluminescence immunoassay reagent; and taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

optionally selecting at least one experimental parameter from the parameters corresponding to the fluorescence immunoassay reagent; and taking a combination mode formed by at least one experimental parameter as an experimental parameter combination mode.

arranging at least one numerical value in each experimental parameter, and setting the numerical value arrangement sequence corresponding to each experimental parameter; and setting the experiment sequence of the experiment parameter combination mode according to the numerical value arrangement sequence corresponding to each experiment parameter.

In one embodiment, a computer program product is provided, comprising a computer program which, when executed by a processor, performs the steps of:

the experimental parameters corresponding to the fluorescence immunoassay reagent comprise: testing sampling position, testing times, sample number, sample type, incubation type, first testing time, testing time interval and experimental sample concentration; optionally selecting at least one experimental parameter from the parameters corresponding to the fluorescence immunoassay reagent; and taking a combination mode formed by at least one experiment parameter as an experiment parameter combination mode.

if the type of the reagent for immunoassay is a chemiluminescence immunoassay reagent, the chemiluminescence immunoassay reagent corresponds to at least one raw material, and each raw material in the at least one raw material comprises at least one dosage, setting the corresponding dosage for each raw material; and taking the combination mode formed by the dosages corresponding to the at least one raw material as an experimental parameter combination mode.

It should be noted that, the user information (including but not limited to user equipment information, user personal information, etc.) and data (including but not limited to data for analysis, stored data, displayed data, etc.) referred to in the present application are information and data authorized by the user or sufficiently authorized by each party, and the collection, use and processing of the related data need to comply with the relevant laws and regulations and standards of the relevant country and region.

It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above can be implemented by hardware instructions of a computer program, which can be stored in a non-volatile computer-readable storage medium, and when executed, can include the processes of the embodiments of the methods described above. Any reference to memory, databases, or other media used in the embodiments provided herein can include at least one of non-volatile and volatile memory. The nonvolatile Memory may include Read-Only Memory (ROM), magnetic tape, floppy disk, flash Memory, optical Memory, high-density embedded nonvolatile Memory, resistive Random Access Memory (ReRAM), magnetic Random Access Memory (MRAM), ferroelectric Random Access Memory (FRAM), phase Change Memory (PCM), graphene Memory, and the like. Volatile Memory can include Random Access Memory (RAM), external cache Memory, and the like. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM), among others. The databases referred to in various embodiments provided herein may include at least one of relational and non-relational databases. The non-relational database may include, but is not limited to, a block chain based distributed database, and the like. The processors referred to in the embodiments provided herein may be general purpose processors, central processing units, graphics processors, digital signal processors, programmable logic devices, quantum computing based data processing logic devices, etc., without limitation.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above examples only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present application shall be subject to the appended claims.

Claims

1. An assay method for reagent development, the method comprising:

setting an experiment sequence of the experiment parameter combination mode, starting an experiment according to the experiment sequence, and automatically performing the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

2. The method according to claim 1, wherein determining the combination of experimental parameters based on the type of the immunoassay reagent comprises:

if the type of the reagent for immunoassay is the chemiluminescence immunoassay reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the chemiluminescence immunoassay reagent;

and if the type of the reagent for the immunodetection is the fluorescence immunoassay reagent, determining an experiment parameter combination mode according to experiment parameters corresponding to the fluorescence immunoassay reagent.

3. The method according to claim 2, wherein the determining the combination of experimental parameters according to the experimental parameters corresponding to the chemiluminescent immunoassay reagent comprises:

optionally selecting at least one experimental parameter from the experimental parameters corresponding to the chemiluminescence immunoassay reagent;

and taking a combination mode formed by the at least one experiment parameter as the experiment parameter combination mode.

4. The method of claim 2, wherein determining the combination of experimental parameters based on the experimental parameters corresponding to the fluorescent immunoassay reagent comprises:

optionally selecting at least one experimental parameter from the experimental parameters corresponding to the fluorescence immunoassay reagent;

5. The method according to claim 2, wherein determining a combination of experimental parameters based on the type of the immunoassay reagent comprises:

if the type of the reagent for immunoassay is the chemiluminescent immunoassay reagent, the chemiluminescent immunoassay reagent corresponds to at least one raw material, and each raw material of the at least one raw material comprises at least one dose, setting the corresponding dose for each raw material;

and taking the combination mode formed by the dosages corresponding to the at least one raw material as the experiment parameter combination mode.

6. The method of claim 3, wherein each of the experimental parameters for the chemiluminescent immunoassay reagent comprises at least one numerical value;

the combination mode formed by the at least one experimental parameter as the experimental parameter combination mode comprises:

and taking a combination mode formed by numerical values corresponding to the at least one experimental parameter as the experimental parameter combination mode.

7. The method according to claim 6, wherein the setting of the experiment sequence of the experiment parameter combination mode comprises:

8. An assay device for reagent development, the device comprising:

the device comprises an acquisition module, a detection module and a control module, wherein the acquisition module is used for acquiring the type of a reagent for immunoassay, and the type of the reagent for immunoassay comprises a chemiluminescence immunoassay reagent or a fluorescence immunoassay reagent;

the determination module is used for determining an experimental parameter combination mode according to the type of the reagent for immunoassay;

the experiment module is used for setting an experiment sequence of the experiment parameter combination mode, starting an experiment according to the experiment sequence and automatically carrying out the experiment according to the experiment sequence; and outputting the experimental result of the reagent for immunoassay after the experiment is finished.

9. A computer device comprising a memory and a processor, the memory storing a computer program, characterized in that the processor, when executing the computer program, implements the steps of the method of any of claims 1 to 7.

10. A computer-readable storage medium, on which a computer program is stored, which, when being executed by a processor, carries out the steps of the method of any one of claims 1 to 7.