CN115161179B - Device and method for testing ecological toxicity of solid waste leachate based on zebra fish embryo - Google Patents
Device and method for testing ecological toxicity of solid waste leachate based on zebra fish embryo Download PDFInfo
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- CN115161179B CN115161179B CN202210756524.8A CN202210756524A CN115161179B CN 115161179 B CN115161179 B CN 115161179B CN 202210756524 A CN202210756524 A CN 202210756524A CN 115161179 B CN115161179 B CN 115161179B
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- 239000002910 solid waste Substances 0.000 title claims abstract description 49
- 241000252212 Danio rerio Species 0.000 title claims abstract description 42
- 238000012360 testing method Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 23
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 14
- 231100000419 toxicity Toxicity 0.000 title description 10
- 230000001988 toxicity Effects 0.000 title description 10
- 239000000969 carrier Substances 0.000 claims abstract description 33
- 238000002386 leaching Methods 0.000 claims abstract description 33
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 24
- 231100000584 environmental toxicity Toxicity 0.000 claims abstract description 14
- 239000011148 porous material Substances 0.000 claims description 47
- 230000034994 death Effects 0.000 claims description 15
- 231100000517 death Toxicity 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 15
- 238000004804 winding Methods 0.000 claims description 11
- 238000004811 liquid chromatography Methods 0.000 claims description 9
- 231100000331 toxic Toxicity 0.000 claims description 8
- 230000002588 toxic effect Effects 0.000 claims description 8
- 231100000673 dose–response relationship Toxicity 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000013558 reference substance Substances 0.000 claims description 6
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 5
- 229910052744 lithium Inorganic materials 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 4
- 230000012447 hatching Effects 0.000 claims description 4
- 210000001325 yolk sac Anatomy 0.000 claims description 4
- SDYWXFYBZPNOFX-UHFFFAOYSA-N 3,4-dichloroaniline Chemical compound NC1=CC=C(Cl)C(Cl)=C1 SDYWXFYBZPNOFX-UHFFFAOYSA-N 0.000 claims description 3
- 238000013016 damping Methods 0.000 claims description 3
- 230000013020 embryo development Effects 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims description 3
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- 238000001514 detection method Methods 0.000 abstract description 12
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 abstract 1
- 238000011047 acute toxicity test Methods 0.000 abstract 1
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- 241001465754 Metazoa Species 0.000 description 3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/30—Landfill technologies aiming to mitigate methane emissions
Abstract
The invention discloses a device and a method for testing the ecotoxicity of solid waste leachate based on zebra fish embryos, wherein the device comprises a shell, two groups of stacked orifice plate carriers which can be pulled out from the shell and unfolded or stored in the shell are arranged in the shell, and the device has the advantages of simplicity in operation, large orifice plate capacity and the like; according to the method, the zebra fish embryo is utilized to carry out an acute toxicity test on the ecotoxicity of the solid waste leaching solution, so that a novel detection mode can be provided for the ecotoxicity detection of the solid waste leaching solution.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a device and a method for testing the ecotoxicity of solid waste leachate based on zebra fish embryos.
Background
Through carrying out leaching toxicity detection to solid waste, can effectually analyze out harmful and toxic substance composition, however current detection operation often wastes time and energy, and the test is put chaotic with the orifice plate to because there is the problem of respecting animal life in some experiments, consequently, a new solid waste lixivium ecotoxicity detection device and method is needed at present to solve above-mentioned problem.
Disclosure of Invention
A device for testing the ecotoxicity of solid waste leachate based on zebra fish embryos comprises a shell, a handle is arranged above the shell, the interior of the handle is hollow,
two groups of stacked orifice plate carriers for carrying multiple groups of orifice plates are arranged in the shell, one group of guide plates corresponding to the stacked orifice plate carriers one by one are respectively arranged on one group of opposite inner walls of the shell, the stacked orifice plate carriers are connected with the guide plates in a sliding way,
the stacked orifice plate carrier comprises a driving connecting plate and a plurality of groups of carrier plates for carrying orifice plates, the carrier plates are parallel to each other and are perpendicular to the driving connecting plate,
a first chute is arranged in the front surface of the driving connecting plate along the length direction, a first bar-shaped hole communicated with the first chute is arranged on the back surface of the driving connecting plate, a second chute is respectively arranged in the left side surface and the right side surface of the driving connecting plate along the length direction, second bar-shaped holes communicated with the first chute are arranged on the inner wall of the second chute, the number of the second bar-shaped holes is the same as that of the carrier plates,
a main air bag is arranged outside the left side surface and the right side surface of the driving connecting plate, one end of the driving connecting plate is provided with a fixed plate used for being connected with one end of the main air bag, the other end of the main air bag is provided with a locking switching block used for switching and locking the shell or the driving connecting plate, the locking switching block is in sliding connection with the driving connecting plate,
each carrier plate is in sliding connection with the driving connecting plate through a guide sliding block positioned in the first sliding groove, the carrier plates are in rotary connection with the guide sliding blocks through shaft rods,
an auxiliary slide block which is in sliding clamping connection with the second bar-shaped hole is arranged on the side surface of the guide slide block, the auxiliary slide block is connected with an auxiliary air bag, the auxiliary slide block and the auxiliary air bag are both positioned in the second sliding groove, one end of the auxiliary air bag penetrates through the driving connecting plate through a connecting pipe and is communicated with a main air bag corresponding to the auxiliary air bag,
the other end of the driving connecting plate is provided with a driving rod for driving the driving connecting plate to rotate, one end of the driving rod penetrates through the driving connecting plate and is in sliding connection with the guide plate, the driving rod is clamped with a rod hole of the driving connecting plate, the other end of the driving rod penetrates through the handle and is provided with a first bevel gear,
the handle is provided with a second bevel gear which is meshed with the first bevel gears of the two groups of stacked orifice plate carriers at the same time, and a plurality of groups of carrier plates of the two groups of stacked orifice plate carriers are arranged in a staggered manner.
According to one aspect of the invention, a winding wheel for winding the pull rope is arranged on the driving rod positioned in the first chute, the carrier plate passes through the guide sliding block through the shaft rod and is connected with the driving gear positioned in the first chute,
one end of the pull rope is fixedly connected with the winding wheel, the other end of the pull rope is provided with a driving sliding block which is in sliding connection with the side wall of the first chute, the driving sliding block is connected with a first telescopic rod which is arranged on the inner bottom surface of one end of the driving connecting plate through at least one group of guide rods,
the guide rod is provided with sliding toothed plates which are used for driving the driving gears to rotate at positions corresponding to the positions of the driving gears, the guide sliding block is provided with a supporting rod which is used for synchronously lifting the sliding toothed plates,
the guide rod is hollow, at least one group of through holes are arranged on the side wall of the guide rod corresponding to the position of the guide sliding block corresponding to the auxiliary air bag in the stretching state, a touch spring is arranged in the guide rod, a locking piece is arranged on one side surface of the touch bar corresponding to the position of the through holes, a first lug is arranged on the other side surface of the touch bar, a second lug for pushing the first lug to enable the locking piece to extend out of the through holes is arranged on the inner wall of the guide rod corresponding to the position of the first lug,
one end of the trigger spring is connected with the driving slide block through a first spring, the side surface of the other side of the trigger spring is connected with the inner wall of the guide rod in a sliding way through a second spring,
the touch spring above the locking piece corresponding to the sliding toothed plate closest to the driving sliding block is provided with a clamping plate for enabling the trigger bar to slide along the direction of the guide rod, the guide rod above the through hole is provided with a third strip-shaped hole for providing a sliding space for the clamping plate, and the inner wall of the rod hole of the sliding toothed plate corresponding to the position of the through hole is provided with a locking hole matched with the through hole.
Through the arrangement, synchronous driving of components such as a pull rope and the like and the driving rod can be realized, so that the driving rod drives the driving connecting plate to rotate by 90 degrees, and simultaneously, each sliding toothed plate is meshed with each corresponding driving gear for transmission, so that each carrier plate is synchronously driven to turn by 90 degrees, and further, the situation that liquid filled in an orifice plate on the carrier plate is inclined and spilled due to the fact that the carrier plate is not in a state of being horizontal at any time during the rotation of the driving connecting plate is prevented,
meanwhile, the position of the guide sliding block corresponding to the auxiliary air bag when the carrier plate is in an extending state and the position of the guide sliding block corresponding to the auxiliary air bag when the carrier plate is in a compressing state can be switched through the cooperation of the main air bag and the auxiliary air bag, in order to meet the switching, when the position of the guide sliding block corresponding to the auxiliary air bag when the carrier plate is in the extending state is set through the components such as a contact spring, the sliding toothed plate is driven to be clamped with the guide rod, and therefore the sliding toothed plate is driven to drive the driving gear through the movement of the pull rope, the driving sliding block and the guide rod, and the functions are achieved.
According to one aspect of the invention, the locking switching block is hollow, a group of first motor push rods for being clamped with a first clamping hole on the shell and a group of second motor push rods for being clamped with a second clamping hole on the driving connecting plate are arranged in the locking switching block, the first clamping hole is arranged on the inner side wall of the shell corresponding to the main air bag in an extending state, the second clamping hole is arranged on the outer side wall of the locking switching block corresponding to the main air bag in a compressing state, a lithium battery and a single chip microcomputer for supplying power and controlling the first motor push rods and the second motor push rods are arranged in the locking switching block, a first trigger button is arranged on the locking switching block corresponding to the position of the shell, a second trigger button is arranged on the locking switching block corresponding to the position of the fixing plate, and the first trigger button and the second trigger button are electrically connected with the single chip microcomputer, the first motor push rods and the second motor push rods.
Through the setting, utilize the singlechip to carry out the control of first motor push rod, second motor push rod to realize when first trigger button triggers the back, open first motor push rod outwards and open second motor push rod inwards shrink simultaneously, when second trigger button triggers the back, open second motor push rod outwards and open first motor push rod inwards shrink simultaneously, thereby satisfy the realization that main gasbag stretched, compression function.
According to one aspect of the invention, a cover plate is arranged on the shell, two groups of support plates for supporting the corresponding side stacked orifice plate carrier are arranged on the cover plate, the support plates are rotatably connected with the cover plate through second telescopic rods, and the second telescopic rods are rotatably connected with the support plates through rotary discs. Through the arrangement, the stability of the stacked orifice plate carrier when the carrier plate is unfolded can be improved, and the stacked orifice plate carrier and the orifice plate on the carrier plate can be prevented from accumulating dust when the stacked orifice plate carrier is not used.
According to one aspect of the invention, the guide plate comprises a plate body fixedly connected with the shell and an extension plate rotatably connected with the top end of the plate body, one end of the extension plate is rotatably connected with the plate body through a damping rotating shaft, and the other end of the extension plate is provided with a positioning pin for being clamped with the driving rod or the handle. Through above-mentioned setting, when can saving guide board occupation space, guarantee to guide the effect that the board provided sliding guide for two sets of stacked orifice plate carriers to further saved the device and accomodate the back occupied space, and it is more convenient to carry.
According to one aspect of the invention, the side surface of the guide slide block corresponding to the second slide groove is also provided with a turned-over edge which is used for sliding contact with the inner wall of the first slide groove, the side surface of the turned-over edge which is contacted with the inner wall of the first slide groove is provided with a first magnetic sheet, the inner wall of the first slide groove is provided with two groups of second magnetic sheets, and the two groups of second magnetic sheets respectively and correspondingly magnetically attract the guide slide block corresponding to the ballonet in the extending state and the guide slide block corresponding to the ballonet in the compressing state. Through the arrangement, the guide sliding blocks corresponding to the auxiliary air bags in the extending state and the guide sliding blocks corresponding to the auxiliary air bags in the compressing state have certain acting force generated by magnetic attraction, and the guide sliding blocks can generate paragraph sense feedback caused by magnetic attraction when the two states are switched when the handle is lifted, so that the operation and the use are more humanized.
According to another aspect of the invention, the invention also provides a method for testing the ecotoxicity of the solid waste leachate by using the device, which comprises the following steps:
step 1: pulling a handle to pull and spread each carrier plate of the two groups of stacked pore plate carriers from the shell, wherein the pore plates are colorless and transparent 24-hole test plates, and each pore plate comprises at least 3 groups of test pore plates, at least 1 group of reference substance comparison pore plates, 1 group of blank comparison pore plates and 1 group of solvent comparison pore plates, and then each pore plate is placed in a plate groove of each carrier plate;
step 2: the solid waste leaching solution is divided into 20 parallel holes of a test pore plate according to the amount of 2mL of each parallel hole,
4.0 mg/L3, 4-dichloroaniline was dispensed into 20 parallel wells of a reference control well plate at a rate of 2mL per parallel well,
the solvent used by the solid waste leaching solution is split into 20 parallel holes of a solvent control pore plate according to the amount of 2mL of each parallel hole,
the rest 4 parallel holes of the test pore plate, the rest 4 parallel holes of the reference substance comparison pore plate, the rest 4 parallel holes of the solvent comparison pore plate and the 24 parallel holes of the blank comparison pore plate are added with culture water according to the amount of 2mL of each parallel hole to serve as blank comparison;
step 3: selecting zebra fish embryos within 2.0hpf after fertilization, and placing the zebra fish embryos with good embryo development in each pore plate by microscopic examination, wherein 1-2 embryos are added into each parallel pore, and the replacement frequency of the solid waste leaching solution, 3, 4-dichloroaniline, the solvent used by the solid waste leaching solution and the culture water added by a blank control is 24 h/time;
step 4: checking the death end point of the zebra fish embryo by a microscope, wherein the judgment standards are that 1) eggs are coagulated, 2) tails are not separated from yolk sacs, 3) heartbeats are not needed, 4) somite development is not needed, and 5) hatching is not needed;
step 5: judging the toxic effect of the solid waste leaching solution according to the death rate of the zebra fish embryos, measuring the death data of the zebra fish embryos for 96 hours, analyzing and drawing a dose-response curve chart of the death data of the zebra fish embryos, and calculating LC (liquid chromatography) 50 Data.
The beneficial effects of the invention are as follows:
(1) The device is provided with two groups of stacked pore plate carriers which can be pulled out from the shell and unfolded or stored in the shell, the capacity of the pore plates carried by the stacked pore plate carriers is large, the occupied area after storage is small, the stacked pore plate carriers are easy to carry, and meanwhile, the stacked pore plate carriers are convenient to switch between the two states, so that a novel detection device is provided for an eco-toxicity detection test of solid waste leaching solutions requiring multiple groups of pore plates.
(2) The method provides a new detection mode for the ecotoxicity detection of the solid waste leaching solution, provides guidance selection for the pH value, organic matters and salt ion content standard of the solid waste leaching solution under the typical environmental condition of the zebra fish embryo toxicity test, reflects the content level of toxic substances in the solid waste from the ecological toxicity of the leaching solution, can be used as a biological supplementing method for identifying the content of the toxic substances in the solid waste leaching solution, and can further reflect the ecological environmental hazard of the solid waste.
(3) Compared with the detection of the embryo mortality of the zebra fish, the method provided by the invention utilizes the embryo mortality to judge the ecological toxicity of the solid waste leaching solution, so that the judging time of the toxicity test of the zebra fish is shortened, and the fish embryo does not belong to the animal welfare category, so that the method has an improvement significance in preventing or reducing the irritation and injury to the adult zebra fish, and respecting the animal life.
Drawings
FIG. 1 is a schematic view showing the appearance of a storage state of the device of the present invention;
FIG. 2 is a schematic view of the device of the present invention in an expanded state;
FIG. 3 is a schematic view of the housing structure of the device of the present invention;
FIG. 4 is a schematic view showing a cover plate accommodating state of the device;
FIG. 5 is a schematic view of the cover plate of the device of the present invention in an expanded configuration;
FIG. 6 is a schematic diagram of the assembly relationship of two sets of stacked orifice plate carriers of the apparatus of the present invention;
FIG. 7 is a schematic view of a stacked orifice plate carrier storage configuration of the apparatus of the present invention;
FIG. 8 is a schematic view of the internal structure of a stacked orifice plate carrier storage state of the apparatus of the present invention;
FIG. 9 is a schematic view of the stacked orifice plate carrier of the apparatus of the present invention in an expanded configuration;
FIG. 10 is a schematic view of the internal structure of a stacked orifice plate carrier of the apparatus of the present invention in an expanded state;
FIG. 11 is a schematic view of the drive connection plate structure of the device of the present invention;
FIG. 12 is a schematic view of the internal structure of a drive connection plate of the apparatus of the present invention;
FIG. 13 is a schematic view of the handle and drive rod assembly of the device of the present invention;
FIG. 14 is a schematic view of the assembly of a first bevel gear with a second bevel gear of the apparatus of the present invention;
FIG. 15 is a schematic view of a carrier structure of the apparatus of the present invention;
FIG. 16 is a schematic diagram of the internal structure of a lock switch block of the apparatus of the present invention;
FIG. 17 is a schematic view of the structure of the device of the present invention in a state of being housed in a string and related components;
FIG. 18 is a schematic view showing the internal structure of the device of the present invention in a state where the pull cord and the related components are housed;
FIG. 19 is a schematic view of the structure of the device of the present invention in its deployed state with respect to the pull cord;
FIG. 20 is a schematic view of the internal structure of the device of the present invention in its deployed state with the pull cord and associated components;
FIG. 21 is a graph of dose-response fit of zebra fish embryonic death data for the method of the invention;
FIG. 22 is a table of confidence limits for the method of the present invention;
FIG. 23 is a schematic representation of egg coagulation at the end of embryonic death of zebra fish in the method of the present invention;
FIG. 24 is a schematic representation of the tail of the death end of zebra fish embryos of the method of the present invention without separation from the yolk sac;
FIG. 25 is a heartbeat-free schematic representation of the end-point of zebra fish embryo death in the method of the present invention;
FIG. 26 is a schematic of the non-hatching of the end-point of death of zebra fish embryos in the method of the present invention;
FIG. 27 is a schematic representation of the development of a lack of somites at the end of embryonic death in a method of the invention, wherein a is normal development and b is lack of somites development;
wherein 1-housing, 11-handle, 12-guide plate, 121-panel body, 122-extension panel, 123-locating pin, 13-second bevel gear, 14-first snap hole,
2-driving connecting plates, 21-first sliding grooves, 22-first strip-shaped holes, 23-second sliding grooves, 24-second strip-shaped holes, 25-fixing plates and 26-second clamping holes,
3-carrier plate, 31-guide slide block, 32-auxiliary slide block, 33-driving gear, 34-supporting rod and 35-flanging,
4-main air bag, 41-auxiliary air bag,
a 5-locking switching block, a 51-first motor push rod, a 52-second motor push rod, a 53-first trigger button and a 54-second trigger button,
6-driving rod, 61-first bevel gear, 62-winding wheel,
7-stay cord, 71-driving slider, 72-guide rod, 721-through hole, 722-third bar hole, 73-sliding toothed plate, 74-touch spring, 741-locking piece, 742-first bump, 743-clamping plate, 75-second bump, 76-first spring, 77-second spring,
8-cover plate, 81-backup pad.
Detailed Description
The invention will be described in further detail with reference to the following embodiments to better embody the advantages of the invention.
Example 1
As shown in fig. 1 and 2, the device for testing the ecotoxicity of the solid waste leaching solution based on the zebra fish embryo comprises a shell 1, a handle 11 is arranged above the shell 1, the interior of the handle 11 is hollow,
as shown in fig. 2, 3 and 7, two groups of stacked orifice plate carriers for carrying twelve groups of orifice plates are arranged in the shell 1, a group of guide plates 12 corresponding to the stacked orifice plate carriers one by one are respectively arranged on the front inner wall and the rear inner wall of the shell 1, the stacked orifice plate carriers are in sliding connection with the guide plates 12, the guide plates 12 comprise a plate body 121 fixedly connected with the shell 1 and an extension plate 122 rotationally connected with the top end of the plate body 121, one end of the extension plate 122 is rotationally connected with the plate body 121 through a damping rotating shaft, and the other end of the extension plate 122 is provided with a positioning pin 123 for being clamped with the driving rod 6 or the handle 11;
as shown in fig. 6, 7 and 9, the stacked orifice plate carrier includes a driving connection plate 2 and three groups of carrier plates 3 for carrying orifice plates, the three groups of carrier plates 3 are parallel to each other and are perpendicular to the driving connection plate 2,
as shown in fig. 8, a first chute 21 is arranged in the front surface of the driving connection plate 2 along the length direction thereof, a first bar-shaped hole 22 communicated with the first chute 21 is arranged on the back surface of the driving connection plate 2, as shown in fig. 8, 11 and 12, a second chute 23 is respectively arranged in the left and right side surfaces of the driving connection plate 2 along the length direction thereof, second bar-shaped holes 24 communicated with the first chute 21 are arranged on the inner wall of the second chute 23, the number of the second bar-shaped holes 24 is the same as that of the carrier plates 3,
as shown in fig. 7, 8 and 9, a main air bag 4 is arranged outside the left and right side surfaces of the driving connecting plate 2, one end of the driving connecting plate 2 is provided with a fixed plate 25 used for being connected with one end of the main air bag 4, the other end of the main air bag 4 is provided with a locking switching block 5 used for switching locking with the shell 1 or the driving connecting plate 2, the locking switching block 5 is in sliding connection with the driving connecting plate 2,
as shown in fig. 16, the locking switch block 5 is hollow, a group of first motor push rods 51 for clamping the first clamping holes 14 on the shell 1 and a group of second motor push rods 52 for clamping the second clamping holes 26 on the driving connection plate 2 are arranged in the locking switch block 5, the first clamping holes 14 are arranged on the inner side wall of the shell 1 corresponding to the main air bag 4 in the extending state, the second clamping holes 26 are arranged on the outer side wall of the locking switch block 5 corresponding to the main air bag 4 in the compressing state, lithium batteries and a singlechip for supplying power and controlling the first motor push rods 51 and the second motor push rods 52 are arranged in the locking switch block 5, a first trigger button 53 is arranged on the locking switch block 5 corresponding to the position of the shell 1, a second trigger button 54 is arranged on the locking switch block 5 corresponding to the position of the fixing plate 25, and the first trigger button 53 and the second trigger button 54 are electrically connected with the singlechip, the first motor push rods 51 and the second motor push rods 52; the single chip microcomputer is a commercially available programmable single chip microcomputer, the lithium battery is a commercially available lithium battery, the first motor push rod 51 and the second motor push rod 52 are commercially available push rod motors or the shapes of the commercially available push rod motors are adjusted so as to be installed in the device in an adaptive manner;
as shown in fig. 8 and 10, each carrier plate 3 is slidably connected with the driving connection plate 2 through a guide slide block 31 positioned in the first chute 21, and the carrier plates 3 are rotatably connected with the guide slide blocks 31 through shaft rods,
as shown in fig. 12, an auxiliary slide block 32 which is in sliding clamping connection with the second bar-shaped hole 24 is arranged on the side surface of the guide slide block 31, the auxiliary slide block 32 is connected with an auxiliary air bag 41, the auxiliary slide block 32 and the auxiliary air bag 41 are both positioned in the second sliding groove 23, the lower end of the auxiliary air bag 41 penetrates through the driving connecting plate 2 through a connecting pipe and is communicated with a main air bag 4 corresponding to the auxiliary air bag 41,
as shown in fig. 7, the other end of the driving connection board 2 is provided with a driving rod 6 for driving the driving connection board 2 to rotate, one end of the driving rod 6 penetrates through the driving connection board 2 and is in sliding connection with the guiding board 12, the driving rod 6 is clamped with a rod hole of the driving connection board 2, the other end of the driving rod 6 penetrates through the handle 11 and is provided with a first bevel gear 61,
as shown in fig. 8, 17-20, a winding wheel 62 for winding the pull rope 7 is arranged on the driving rod 6 positioned in the first chute 21, the carrier plate 3 passes through the guide slide block 31 through a shaft rod and is connected with the driving gear 33 positioned in the first chute 21, one end of the pull rope 7 is fixedly connected with the winding wheel 62, the other end of the pull rope 7 is provided with a driving slide block 71 which is in sliding connection with the side wall of the first chute 21, the driving slide block 71 is connected with a first telescopic rod arranged on the inner bottom surface of one end of the driving connecting plate 2 through two groups of guide rods 72, sliding toothed plates 73 for driving the driving gear 33 to rotate are arranged on the positions of the guide rods 72 corresponding to the positions of the driving gears 33, a supporting rod 34 for synchronously lifting the sliding toothed plates 73 is arranged on the guide slide block 31, the guide rods 72 are hollow, three groups of through holes 721 are arranged on the side wall of the guide rod 72 corresponding to the position of the guide slide block 31 corresponding to the auxiliary airbag 41 in the stretching state, a trigger spring 74 is arranged in the guide rod 72, a locking piece 741 is arranged on one side surface of the trigger bar 74 corresponding to the position of the through hole 721, a first lug 742 is arranged on the other side surface of the trigger bar 74, a second lug 75 for pushing the first lug 742 to enable the locking piece 741 to extend out of the through hole 721 is arranged on the inner wall of the guide rod 72 corresponding to the position of the first lug 742, one end of the trigger bar 74 is connected with the driving slide block 71 through a first spring 76, the other side surface of the trigger bar 74 is connected with the inner wall of the guide rod 72 in a sliding way through a second spring 77, a clamping plate 743 for enabling the trigger bar 74 to slide along the direction of the guide rod 72 is arranged on the trigger bar 74 above the sliding toothed plate 73 closest to the driving slide block 71, a third bar-shaped hole 722 for providing a sliding space for the clamping plate 743 is arranged on the guide rod 72 above the through hole 721, the inner wall of the rod hole of the sliding toothed plate 73 corresponding to the position of the through hole 721 is provided with a lock hole matched with the through hole 721;
as shown in fig. 13 and 14, the handle 11 is provided with a second bevel gear 13 which is engaged with and driven by the first bevel gears 61 of the two groups of stacked orifice plate carriers, and the multiple groups of carrier plates 3 of the two groups of stacked orifice plate carriers are staggered with each other.
The working principle of the device is as follows:
pulling the positioning pin 123 apart, then rotating the extension plate 122 of each of the two sets of guide plates 12 by 90 degrees to unfold the extension plate 122 and the plate body 121, then lifting the handle 11 upwards, sliding the two sets of stacked orifice plate carriers upwards along the guide plates 12 by pulling the handle 11 until sliding to the top of the extension plate 122, then pressing the positioning pin 123 to rotationally clamp and limit the drive rod 6,
because the two groups of stacked orifice plates have the same structure and the same principle, the description mode of a single group of stacked orifice plates is adopted for description of the principle, when the stacked orifice plates slide upwards along the guide plate 12, the main air bag 4 is extruded by the fixed plate 25 of the driving connecting plate 2 in cooperation with the locking switching block 5, during the period, under the communication effect of the main air bag 4 and the corresponding auxiliary air bags 41, the auxiliary air bags 41 are stretched and the corresponding carrier plates 3 are driven to move upwards by the guiding sliding blocks 31 through the cooperation effect of the auxiliary sliding blocks 32, after the protrusion of the fixed plate 25 is triggered by the pressing of the second triggering button 54, the second motor push rod 52 is outwards extended and the first motor push rod 51 is simultaneously opened to shrink inwards under the program of the singlechip, so that the locking switching block 5 is switched from the clamping state with the first clamping hole 14 of the shell 1 to the clamping state with the second clamping hole 26 of the driving connecting plate 2,
thereby, the corresponding heights of the carrier plates 3 of the two groups of stacked orifice plate carriers are lifted (the heights are determined by the length of the stretching state of the auxiliary air bag 41, corresponding adjustment and arrangement are carried out according to the actual arrangement), effective spacing distances are provided for the subsequent unfolding of the carrier plates 3, meanwhile, during the lifting movement of the guide sliding block 31, the corresponding sliding toothed plate 73 is lifted under the action of the supporting rod 34, when the uppermost sliding toothed plate 73 pushes the clamping plate 743 of the contact spring 74 to lift upwards and squeeze the first spring 76, the first protruding block 742 moves to the second protruding block 75, the trigger spring 74 stretches the second spring 77 under the pushing action of the second protruding block 75, the locking block 741 stretches out of the through hole 721 to be in clamping connection with the locking hole of the sliding toothed plate 73, and meanwhile, when the clamping plate 743 lifts upwards to the upper end of the third strip-shaped hole 722, the clamping plate 743 is clamped with the clamping groove arranged at the upper end of the sliding toothed plate 73, and locking of the guide rod 72 and the sliding toothed plate 73 is completed; when the guide slider 31 falls, the sliding toothed plate 73 loses the lifting force of the guide slider 31, and the components can be reset under the restoring force of the first spring 76 and the second spring 77 in the same principle as the above;
subsequently, the second bevel gears 13 are rotated to respectively drive the driving rods 6 of the two groups of stacked orifice plate carriers to rotate 90 degrees clockwise and 90 degrees anticlockwise, so that the two groups of stacked orifice plate carriers are unfolded to the left and right sides by taking the handle 11 as the center,
meanwhile, when the winding wheel 62 of the driving rod 6 winds the pull rope 7, the driving sliding block 71 and the guide rod 72 are pulled upwards, the guide rod 72 compensates the distance between the driving connecting plate 2 through the first telescopic rod during the upward movement of the guide rod 72, and each sliding toothed plate 73 is driven to move upwards, so that each carrier plate 3 is rotated under the meshing transmission effect of the sliding toothed plate 73 and the driving gear 33, and the carrier plates 3 are synchronously rotated from 0 DEG to 90 DEG after the clockwise rotation or the anticlockwise rotation of 90 DEG of the driving rod 6 is completed;
then, by stirring each carrier plate 3, the magnetic coatings on the contact surfaces of two adjacent groups of carrier plates 3 are utilized to carry out mutual magnetic attraction, so that each carrier plate 3 is kept to be horizontally unfolded, and an ecological toxicity detection test of the solid waste leaching liquid can be started;
when the storage is needed, the push-back operation is performed in the same principle as the above, and the push-back operation is carried by the handle 11, so that the push-back operation is convenient to move, carry and operate and use in various scenes.
Example 2
The embodiment is different from embodiment 1 in that the invention also provides a method for testing the ecotoxicity of the solid waste leaching solution by using the device, which comprises the following steps:
step 1: pulling the handle 11 to pull and spread each carrier plate 3 of the two groups of stacked pore plate carriers from the shell 1, wherein the pore plates are colorless and transparent 24-pore test plates, and each pore plate comprises at least 3 groups of test pore plates, at least 1 group of reference substance comparison pore plates, 1 group of blank comparison pore plates and 1 group of solvent comparison pore plates, and then each pore plate is placed in a plate groove of each carrier plate 3;
step 2: split charging solid waste leaching solution into 20 parallel holes of a test pore plate according to the amount of 2mL of each parallel hole, wherein the pH value of the solid waste leaching solution is between 4.50 and 10.50, the organic matter content of the solid waste leaching solution is between 0 and 80mg/L, and the salt ion concentration of the solid waste leaching solution is between 0 and 8 mmol/L;
4.0 mg/L3, 4-dichloroaniline was dispensed into 20 parallel wells of a reference control well plate at a rate of 2mL per parallel well,
the solvent used by the solid waste leaching solution is split into 20 parallel holes of a solvent control pore plate according to the amount of 2mL of each parallel hole,
the rest 4 parallel holes of the test pore plate, the rest 4 parallel holes of the reference substance comparison pore plate, the rest 4 parallel holes of the solvent comparison pore plate and the 24 parallel holes of the blank comparison pore plate are added with culture water according to the amount of 2mL of each parallel hole to serve as blank comparison; the water for cultivation is tap water which is subjected to aeration treatment for more than 24 hours, the pH value is 6-8, and the temperature is 26-28 ℃;
step 3: selecting zebra fish embryos within 2.0hpf after fertilization, and placing the zebra fish embryos with good embryo development in each pore plate by microscopic examination, wherein 1-2 embryos are added into each parallel pore, and the replacement frequency of the solid waste leaching solution, 3, 4-dichloroaniline, the solvent used by the solid waste leaching solution and the culture water added by a blank control is 24 h/time;
step 4: microscopic examination of the death endpoint of zebra fish embryos, as shown in fig. 23-27, the criteria were five items, 1) egg coagulation, 2) tail not detached from yolk sac, 3) no heart beat, 4) lack of somite development, 5) no hatching;
step 5: judging the toxic effect of the solid waste leaching solution according to the death rate of the zebra fish embryos, measuring the death data of the zebra fish embryos for 96 hours, analyzing and drawing a dose-response curve chart of the death data of the zebra fish embryos, and calculating LC (liquid chromatography) 50 The data are specifically determined by the following criteria:
1) Extremely toxic: when zebra fish embryo LC 50 When the concentration of the solid waste leaching solution is less than 1mg/L, judging that the ecological toxicity of the solid waste leaching solution is extremely toxic;
2) High toxicity: when zebra fish embryo LC 50 When the concentration of the solid waste leachate is more than or equal to 1mg/L and less than 10mg/L, judging that the ecological toxicity of the solid waste leachate is high;
3) Poisoning: when zebra fish embryo LC 50 When the concentration of the solid waste leachate is more than or equal to 10mg/L and less than 100mg/L, determining that the solid waste leachate is toxic;
4) Low toxicity: when zebra fish embryoLC 50 When the concentration of the solid waste leachate is more than or equal to 100mg/L and less than 1000mg/L, judging that the ecological toxicity of the solid waste leachate is low;
5) Slightly toxic or nontoxic: when the LC50 of the zebra fish embryo is more than 1000mg/L, judging that the ecological toxicity of the solid waste leaching solution is slightly toxic or nontoxic;
the LC50 value is calculated by adopting a probability unit method-SPSS, and the dose-response fitting curve of the zebra fish embryo death data is calculated by adopting the probability unit method-SPSS, wherein the calculation process is as follows:
example 3
The difference between this embodiment and embodiment 1 is that, as shown in fig. 1, 2, 4, and 5, a cover plate 8 is provided on the housing 1, two sets of support plates 81 for supporting the stacked orifice plate carriers on the corresponding side are provided on the cover plate 8, the support plates 81 are rotatably connected with the cover plate 8 through second telescopic rods, and the second telescopic rods are rotatably connected with the support plates 81 through turntables.
The working principle of the device is as follows: the working principle of the present invention is different from that of embodiment 1 in that after the two sets of stacked orifice plate carriers are unfolded, the cover plate 8 is covered on the housing 1, and the two sets of support plates 81 are rotated by 90 ° respectively, and then the second telescopic rods are rotated and stretched to make the two sets of support plates 81 abut against the lower bottom surfaces of the support plates 3 of the stacked orifice plate carriers on the respective corresponding sides respectively, so that the support effect of the cover plate 8 on the stacked orifice plate carriers is achieved.
Example 4
The difference between this embodiment and embodiment 1 is that, as shown in fig. 15, the side surface of the guide slider 31 corresponding to the position of the second chute 23 is further provided with a flange 35 for sliding contact with the inner wall of the first chute 21, the side surface of the flange 35 contacting with the inner wall of the first chute 21 is provided with a first magnetic sheet, and the inner wall of the first chute 21 is provided with two sets of second magnetic sheets, which respectively magnetically attract the guide slider 31 corresponding to the ballonet 41 in the extended state and the guide slider 31 corresponding to the ballonet 41 in the compressed state.
The working principle of the device is as follows: the operation principle is different from that of embodiment 1 in that, when the guide slider 31 corresponding to the sub-air bag 41 in the compressed state is switched to the guide slider 31 corresponding to the sub-air bag 41 in the extended state, feedback generated by the magnetic attraction and the magnetic attraction can be sensed by pulling the handle 11, so that the operator can determine whether the handle 11 is pulled up at this time through the tactile feedback.
Experimental example
The method of the present invention was demonstrated by testing the ecotoxicity of the solid waste leachate using the apparatus of example 1 and the method of example 2.
After 96h of incubation, 96h of zebra fish embryo death data were determined and analyzed to draw a dose-response curve, LC was calculated 50 The data set is used to determine, based on the data,
LC calculation using probability unit method-SPSS 50 The values were calculated as follows:
| concentration mg/L | Log dose | Number of deaths | Total number of | Mortality rate% | Correcting mortality |
| Control | / | 0 | 20 | 0 | 0 |
| 20 | 1.30 | 0 | 20 | 0 | 0 |
| 24 | 1.38 | 2 | 20 | 10 | 10 |
| 28.8 | 1.46 | 8 | 20 | 40 | 40 |
| 34.6 | 1.54 | 14 | 20 | 70 | 70 |
| 41.5 | 1.62 | 20 | 20 | 100 | 100 |
The dose-response fitting curve shown in FIG. 10 was obtained by probability unit method-SPSS calculation, thereby obtaining LC according to the confidence limit table shown in FIG. 11 50 30.392mg/L,95% confidence limit = 27.790-33.366 mg/L.
Claims (6)
1. The device for testing the ecotoxicity of the solid waste leaching solution based on the zebra fish embryo is characterized by comprising a shell (1), a handle (11) is arranged above the shell (1), the interior of the handle (11) is hollow,
two groups of stacked orifice plate carriers for carrying a plurality of groups of orifice plates are arranged in the shell (1), a group of guide plates (12) corresponding to the stacked orifice plate carriers one by one are respectively arranged on one group of opposite inner walls of the shell (1), the stacked orifice plate carriers are in sliding connection with the guide plates (12),
the stacked orifice plate carrier comprises a driving connecting plate (2) and a plurality of groups of carrier plates (3) for carrying the orifice plates, wherein the plurality of groups of carrier plates (3) are parallel to each other and are perpendicular to the driving connecting plate (2),
a first chute (21) is arranged in the front surface of the driving connecting plate (2) along the length direction thereof, first strip-shaped holes (22) communicated with the first chute (21) are arranged on the back surface of the driving connecting plate (2), a second chute (23) is respectively arranged in the left side surface and the right side surface of the driving connecting plate (2) along the length direction thereof, second strip-shaped holes (24) communicated with the first chute (21) are arranged on the inner wall of the second chute (23), the number of the second strip-shaped holes (24) is the same as that of the carrier plates (3),
a main air bag (4) is arranged outside the left side surface and the right side surface of the driving connecting plate (2), one end of the driving connecting plate (2) is provided with a fixed plate (25) used for being connected with one end of the main air bag (4), the other end of the main air bag (4) is provided with a locking switching block (5) used for switching locking with the shell (1) or the driving connecting plate (2), the locking switching block (5) is in sliding connection with the driving connecting plate (2),
each carrier plate (3) is in sliding connection with the driving connecting plate (2) through a guide sliding block (31) positioned in the first sliding groove (21), the carrier plates (3) are in rotary connection with the guide sliding blocks (31) through shaft rods,
an auxiliary sliding block (32) which is in sliding clamping connection with the second strip-shaped hole (24) is arranged on the side surface of the guide sliding block (31), an auxiliary air bag (41) is connected to the auxiliary sliding block (32), the auxiliary sliding block (32) and the auxiliary air bag (41) are both positioned in the second sliding groove (23), one end of the auxiliary air bag (41) penetrates through the driving connecting plate (2) through a connecting pipe and is communicated with a main air bag (4) corresponding to the auxiliary air bag (41),
the other end of the drive connecting plate (2) is provided with a drive rod (6) for driving the drive connecting plate (2) to rotate, one end of the drive rod (6) penetrates through the drive connecting plate (2) and is in sliding connection with the guide plate (12), the drive rod (6) is clamped with a rod hole of the drive connecting plate (2), the other end of the drive rod (6) penetrates through the handle (11) and is provided with a first bevel gear (61),
the handle (11) is provided with a second bevel gear (13) which is meshed and driven with the first bevel gears (61) of the two groups of stacked orifice plate carriers at the same time, and the multiple groups of carrier plates (3) of the two groups of stacked orifice plate carriers are arranged in a staggered manner;
a winding wheel (62) for winding the pull rope (7) is arranged on the driving rod (6) positioned in the first sliding groove (21), the carrier plate (3) passes through the guide sliding block (31) through a shaft rod and is connected with a driving gear (33) positioned in the first sliding groove (21),
one end of the pull rope (7) is fixedly connected with the winding wheel (62), the other end of the pull rope (7) is provided with a driving sliding block (71) which is in sliding connection with the side wall of the first sliding groove (21), the driving sliding block (71) is connected with a first telescopic rod which is arranged on the inner bottom surface of one end of the driving connecting plate (2) through at least one group of guide rods (72),
a sliding toothed plate (73) for driving the driving gears (33) to rotate is arranged at the positions of the guide rods (72) corresponding to the positions of the driving gears (33), a supporting rod (34) for synchronously lifting the sliding toothed plate (73) is arranged on the guide sliding block (31),
the guide rod (72) is hollow, at least one group of through holes (721) are arranged on the side wall of the guide rod (72) corresponding to the position of the guide sliding block (31) corresponding to the auxiliary air bag (41) in the stretching state, a touch spring (74) is arranged in the guide rod (72), a locking piece (741) is arranged on the side surface of one side of the touch bar (74) corresponding to the position of the through hole (721), a first protruding block (742) is arranged on the side surface of the other side of the touch bar (74), a second protruding block (75) for pushing the first protruding block (742) to enable the locking piece (741) to extend out of the through hole (721) is arranged on the inner wall of the guide rod (72) corresponding to the position of the first protruding block (742),
one end of the trigger bar (74) is connected with the driving slide block (71) through a first spring (76), the side surface of the other side of the trigger bar (74) is connected with the inner wall of the guide rod (72) in a sliding way through a second spring (77),
a clamping plate (743) for enabling the triggering strip (74) to slide along the direction of the guide rod (72) is arranged on the triggering strip (74) above the locking block (741) corresponding to the sliding toothed plate (73) closest to the driving sliding block (71), a third strip-shaped hole (722) for providing a sliding space for the clamping plate (743) is arranged on the guide rod (72) above the through hole (721), and a locking hole matched with the through hole (721) is arranged on the inner wall of the rod hole of the sliding toothed plate (73) corresponding to the position of the through hole (721);
the locking switch block (5) is hollow, a group of first motor push rods (51) which are used for being clamped with the first clamping holes (14) in the shell (1) and a group of second motor push rods (52) which are clamped with the second clamping holes (26) in the driving connecting plate (2) are arranged in the locking switch block (5), the first clamping holes (14) are arranged on the inner side wall of the shell (1) corresponding to the main air bag (4) in an extending state, the second clamping holes (26) are arranged on the outer side wall of the locking switch block (5) corresponding to the main air bag (4) in a compressing state, a lithium battery and a single chip microcomputer which are used for supplying power to and controlling the first motor push rods (51) and the second motor push rods (52) are arranged in the locking switch block (5) corresponding to the position of the shell (1), a second trigger button (54) is arranged on the locking switch block (5) corresponding to the position of the fixing plate (25), and the first trigger button (53), the second trigger button (54) and the second trigger button (52) are electrically connected with the first push rods (52) and the second push rods (52).
2. The device according to claim 1, characterized in that the cover plate (8) is arranged on the shell (1), two groups of support plates (81) for supporting the corresponding side stacking type orifice plate carrier are arranged on the cover plate (8), the support plates (81) are rotatably connected with the cover plate (8) through second telescopic rods, and the second telescopic rods are rotatably connected with the support plates (81) through rotary discs.
3. The device according to claim 1, characterized in that the guide plate (12) comprises a plate body (121) fixedly connected with the housing (1), and an extension plate (122) rotatably connected with the top end of the plate body (121), one end of the extension plate (122) is rotatably connected with the plate body (121) through a damping rotating shaft, and the other end of the extension plate (122) is provided with a positioning pin (123) for being clamped with the driving rod (6) or the handle (11).
4. The device according to claim 1, wherein a flange (35) for sliding contact with the inner wall of the first chute (21) is further provided on the side of the guide slider (31) corresponding to the position of the second chute (23), a first magnetic sheet is provided on the side of the flange (35) contacting with the inner wall of the first chute (21), two sets of second magnetic sheets are provided on the inner wall of the first chute (21), and the two sets of second magnetic sheets are respectively magnetically attracted with the guide slider (31) corresponding to the ballonet (41) in the extended state and the guide slider (31) corresponding to the ballonet (41) in the compressed state.
5. The device according to claim 1, characterized in that the top end of the plate body (121) of the guide plate (12) is provided with an extension plate (122) which is rotatably connected to the plate body (121).
6. The method for testing the ecotoxicity of the solid waste leachate by using the device according to any one of claims 1 to 5, comprising the following steps:
step 1: pulling a handle (11) to pull and spread each carrier plate (3) of two groups of stacked orifice plate carriers from a shell (1), wherein the orifice plates are colorless and transparent 24-hole test plates, and comprise at least 3 groups of test orifice plates, at least 1 group of reference substance comparison orifice plates, 1 group of blank comparison orifice plates and 1 group of solvent comparison orifice plates, and then placing each orifice plate in a plate groove of each carrier plate (3);
step 2: the solid waste leaching solution is divided into 20 parallel holes of the test pore plate according to the amount of 2mL of each parallel hole,
4.0 mg/L3, 4-dichloroaniline was dispensed into 20 parallel wells of the reference control well plate in an amount of 2mL per parallel well,
the solvent used by the solid waste leaching solution is split into 20 parallel holes of the solvent control pore plate according to the amount of 2mL of each parallel hole,
the rest 4 parallel holes of the test pore plate, the rest 4 parallel holes of the reference substance control pore plate, the rest 4 parallel holes of the solvent control pore plate and the 24 parallel holes of the blank control pore plate are added with culture water according to the amount of 2mL of each parallel hole to serve as blank control;
step 3: selecting zebra fish embryos within 2.0hpf after fertilization, and placing the zebra fish embryos with good embryo development in each pore plate by microscopic examination, wherein 1-2 embryos are added into each parallel pore, and the replacement frequency of the solvent used by solid waste leaching liquid, 3, 4-dichloroaniline and culture water added by blank control is 24 h/time;
step 4: checking the death end point of the zebra fish embryo by a microscope, wherein the judgment standards are that 1) eggs are coagulated, 2) tails are not separated from yolk sacs, 3) heartbeats are not needed, 4) somite development is not needed, and 5) hatching is not needed;
step 5: judging the toxic effect of the solid waste leaching solution according to the death rate of the zebra fish embryos, measuring the death data of the zebra fish embryos for 96 hours, analyzing and drawing a dose-response curve chart of the death data of the zebra fish embryos, and calculating LC (liquid chromatography) 50 Data.
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| CN202210756524.8A CN115161179B (en) | 2022-06-29 | 2022-06-29 | Device and method for testing ecological toxicity of solid waste leachate based on zebra fish embryo |
| JP2022111020A JP7185114B1 (en) | 2022-06-29 | 2022-07-11 | Apparatus and method for testing ecotoxicity of solid waste exudates based on zebrafish embryos |
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| CN111189999A (en) * | 2019-12-31 | 2020-05-22 | 中国水产科学研究院 | Method for evaluating influence of pollutants on biotoxicity of zebra fish |
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| CN111394246A (en) * | 2020-03-26 | 2020-07-10 | 浙江省农业科学院 | Fish embryo collecting and culturing device and method for evaluating toxicity of thiamethoxam and tetraconazole combined effect |
| CN112986512B (en) * | 2021-02-03 | 2021-12-03 | 生态环境部南京环境科学研究所 | Method for testing ecological toxicity of solid waste leachate based on acute toxicity test of zebra fish |
| CN115161179B (en) * | 2022-06-29 | 2023-05-19 | 生态环境部南京环境科学研究所 | Device and method for testing ecological toxicity of solid waste leachate based on zebra fish embryo |
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2022
- 2022-06-29 CN CN202210756524.8A patent/CN115161179B/en active Active
- 2022-07-11 JP JP2022111020A patent/JP7185114B1/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111189999A (en) * | 2019-12-31 | 2020-05-22 | 中国水产科学研究院 | Method for evaluating influence of pollutants on biotoxicity of zebra fish |
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| CN115161179A (en) | 2022-10-11 |
| JP2024006822A (en) | 2024-01-17 |
| JP7185114B1 (en) | 2022-12-07 |
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