CN115160035B - Preparation method of microbial fertilizer - Google Patents

Preparation method of microbial fertilizer Download PDF

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CN115160035B
CN115160035B CN202210732087.6A CN202210732087A CN115160035B CN 115160035 B CN115160035 B CN 115160035B CN 202210732087 A CN202210732087 A CN 202210732087A CN 115160035 B CN115160035 B CN 115160035B
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fermentation
seed
culture
culture medium
medium
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CN115160035A (en
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肖依文
朱笃
商紫钦
张志斌
汪涯
高波良
吴文婷
罗健
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Jiangxi Science and Technology Normal University
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    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
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    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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    • C12N1/14—Fungi; Culture media therefor
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    • C12R2001/07—Bacillus
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    • C12R2001/80—Penicillium
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

The invention relates to a preparation method of microbial fermentation bacterial manure, which is prepared by compounding chaetomium globosum, bacillus amyloliquefaciens (BNCC 223006), eupenicillium javanicum and burkholderia gladiolus according to a certain proportion and fermenting crop straws or vinasse serving as substrates. The 4 microbial thalli are mixed according to the volume ratio of 1:1:1:1, and the compound microbial fertilizer is prepared by fermenting cheap biomasses such as crop straws or vinasse and the like degraded by microbial fungi on the basis. The microbial fertilizer disclosed by the invention can enable thalli to survive in soil, can decompose organic substances to form humus and release nutrients, provides nutrition for plants, and can also secrete secondary metabolites with the capability of improving the stress resistance of the plants.

Description

Preparation method of microbial fertilizer
Technical Field
The invention relates to the field of fungus fermentation technology and microbial fertilizers, in particular to a preparation method of a microbial fertilizer.
Background
China is a large country of agricultural production, and aims to meet the demands of people on agricultural products. There has long been a need for a large amount of fertilizers and pesticides to greatly increase crop yield. However, excessive use of chemical fertilizers and pesticides can cause serious environmental pollution problems. Such as water eutrophication, groundwater pollution, soil hardening, etc. Plant parasitic nematodes, especially root-knot nematodes, are one of the main pests causing serious yield loss to crops and cash crops, and screening to obtain antagonistic fungi is an effective means for preventing and controlling root-knot nematodes.
The microbial fertilizer is a biological fertilizer containing active microorganisms and capable of improving the fertilizer utilization rate, improving the plant nutrition condition, promoting the plant growth and improving the stress resistance of plants in the using process. The traditional microbial fertilizer has a plurality of limitations in the use process, such as high requirements of active microorganisms on soil environment, low colonization efficiency in soil and unstable effect in fields. In addition, the traditional microbial fertilizer has higher cost in the preparation process, and the produced microbial fertilizer has single effect and cannot be accepted by vast farmers. Therefore, the development of the compound microbial fertilizer with the functions of biological control and plant growth promotion and stress resistance has great practical significance.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a preparation method of a microbial fertilizer by adding 4 microorganisms (Chaetomium globosum DX-THS3 (Chaetomium globosum DX-THS 3), with a preservation number of CCTCC NO: M2016005, preserved in China center for type culture collection at 1 month 4 of 2016, with a preservation address of university of Wuhan, bacillus amyloliquefaciens (BNCC 223006) and Penicillium javanicum R57 (Eupenicillium javanicum R), with a preservation number of CCTCC NO: M2019831, preserved in China center for type culture collection at 10 month 17 of 2019, with a preservation address of university of Wuhan and Burkholderia gladioli Fse32 (Burkholderia gladioli Fse 32), with a preservation number of CCTCC NO: M2014672, preserved in China center for type culture collection at 12 month 28 of 2014, and with a preservation address of university of Wuhan).
In order to achieve the above object, the present invention provides the following technical solutions:
The invention provides a preparation method of a microbial fertilizer, which comprises the following steps:
(1) Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. And (3) picking the bacillus amyloliquefaciens with the inoculating loop, and placing the bacillus amyloliquefaciens with the 2-3 loops on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension. And (3) picking the Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culture to obtain a Burkholderia gladioli Fse seed suspension.
(2) Mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed liquid, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of the nitrogen source in the fermentation medium is 2.0-9.0 g/L;
(3) Performing amplified fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 8-12% v/v, wherein the carbon source of the amplified fermentation culture medium comprises one of rice straw powder, wheat straw powder, corn straw powder and vinasse. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer.
Preferably, the seed culture medium in the step (1) mainly comprises 180-220 g/L of potatoes, 18.0-22.0 g/L of glucose and 0.1-0.3 g/L of yeast extract. The nutrient gravy medium comprises the following components: beef extract 3.0g/L, peptone 10.0g/L, naCl 5.0g/L,5.0mg/L MnSO 4·H2 O, pH 7.0, 121℃and sterilization for 15 min.
Preferably, the fermentation medium in step (2) uses water as a solvent, and comprises the following components in concentration: 4.0-6.0 g/L of sucrose, 3.0-4.0 g/L of KH 2PO4, 0.00002-0.00004 g/L of nitrogen source 2.0～4.0g/L,NaCl 0.4～0.6g/L,MgSO4·7H2O 0.4～0.6g/L,ZnSO4·7H2O 0.002～0.003g/L,NaMoO4·2H2O 0.0002～0.0003g/L,MnSO4·H2O 0.001～0.003g/L,CaCl2 0.01～0.02g/L and H 3BO3.
Preferably, the inoculation amount of the fermentation culture in the step (2) is 2-4%;
the temperature of the fermentation culture is 25-30 ℃;
the fermentation culture time is 6-10 d;
The fermentation culture is shaking culture, and the frequency of shaking is 120-180 rpm.
Preferably, each 2.5L of the medium for the amplified fermentation culture in the step (3) comprises 12.0-13.0 g of sucrose, 6.0-10.0 g of KH 2PO4 and peptone 17.0～18.0g,NaCl 1.0～2.0g,MgSO4·7H2O 1.0～2.0g,ZnSO4·7H2O 0.007～0.008g,NaMoO4·2H2O 0.0005～0.0007g,MnSO4·H2O 0.004～0.006g,CaCl2 0.03～0.04g,H3BO3 0.00007～0.00008g.
Preferably, the amplifying fermentation culture process in the step (3) further comprises a stirring step, wherein the stirring frequency is 180-220 rpm, and the stirring aeration quantity is 0.5-1.5 vvm.
Preferably, the temperature of the amplified fermentation culture in the step (3) is 25-30 ℃, and the time of the amplified fermentation culture is 10-14 d.
Compared with the prior art, the invention has the following beneficial effects:
The strain is endophyte separated from wild rice in Dongxiang in laboratory, and has good activity and high colonization efficiency compared with common wild strain. The raw materials used in the preparation process are low-cost raw materials such as crop straws or waste lees produced in the production process, so that the preparation cost of the microbial fertilizer is reduced. The seed suspension prepared by using 4 strains is mixed and cultured in different proportions, and the obtained microbial fertilizer has various effects and is suitable for different agricultural scenes. The invention has remarkable effect on improving the stress resistance of plants, in particular to inhibiting sclerotinia sclerotiorum and root knot nematodes. Living substances such as indoleacetic acid, phosphorus dissolving and the like generated by the microbial fertilizer prepared by the invention can regulate plant growth, and the dry weight, plant height, root length and the like of the inoculated plants are obviously improved (shown in figures 1,2,3 and 4).
While the invention has been described with respect to what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but on the contrary, is intended to cover various modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Drawings
FIG. 1 is a graph showing a comparative test of the good inhibitory effect of a compound microbial fertilizer on Sclerotinia sclerotiorum.
FIG. 2 the compound microbial fertilizer can be metabolized to produce IAA.
FIG. 3 shows the phosphorus dissolution effect of the compound microbial fertilizer.
FIG. 4 shows the growth of rice plants cultivated with sterile water compared with rice plants after microbial fertilizer (A control group sterile water; B microbial fertilizer).
Detailed Description
The invention will be described in detail with reference to examples. The scope of the invention is not limited in this respect.
Example 1
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9g/L; and carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is rice straw powder, the nitrogen source is wheat bran, and the mass ratio of the straw to the wheat bran is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the antipathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (D).
Example 2
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are picked by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9.0g/L; and carrying out amplified fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplified fermentation culture medium is wheat straw powder, the nitrogen source is wheat bran, and the mass ratio of the wheat straw to the wheat bran is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the antipathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (E).
Example 3
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9.0g/L; and carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is corn straw powder, the nitrogen source is wheat bran, and the mass ratio of the corn straw to the wheat bran is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the antipathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (F).
Example 4
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9.0g/L; and (3) carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is vinasse, the nitrogen source is wheat bran, and the mass ratio of the vinasse to the wheat bran is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The properties against pathogenic fungi are shown in Table 1.
Comparative example 1
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9.0g/L; and (3) carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is rice straw powder, the nitrogen source is bean pulp, and the mass ratio of the rice straw to the bean pulp is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the anti-pathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (a).
Comparative example 2
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9.0g/L; and carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is wheat straw powder, the nitrogen source is bean pulp, and the mass ratio of the wheat straw to the bean pulp is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the anti-pathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (C).
Comparative example 3
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9g/L; and carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is corn straw powder, the nitrogen source is bean pulp, and the mass ratio of the corn straw to the bean pulp is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The performance of the anti-pathogenic fungi is shown in table 1, and the inhibition effect of the compound microbial fertilizer on sclerotinia is shown in fig. 1 (B).
Comparative example 4
Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; 2-3 loops of the eupenicillium javanicum R57 are selected by an inoculating loop and placed on a seed culture medium for shake culture, so as to obtain the eupenicillium javanicum R57 seed suspension. Picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension; mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed solution, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of nitrogen source in the fermentation medium is 9g/L; and (3) carrying out amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of the amplification fermentation culture medium is vinasse, the nitrogen source is soybean meal, and the mass ratio of the vinasse to the soybean meal is 2:1. The water content of the culture medium is 70%, and the fermentation time is 2 weeks. Amplifying fermentation culture to obtain the composite microbial fermentation bacterial fertilizer. The properties against pathogenic fungi are shown in Table 1.
TABLE 1 comparison of the Properties of antipathogenic fungi at different mixing ratios

Claims (1)

1. The preparation method of the microbial fertilizer is characterized by comprising the following steps:
(1) Picking 2-3 chaetomium globosum DX-THS3 by using an inoculating loop, and placing the chaetomium globosum DX-THS3 on a seed culture medium for shake culture to obtain chaetomium globosum DX-THS3 seed suspension; picking 2-3 loops of eupenicillium javanicum R57 by using an inoculating loop, and placing the eupenicillium javanicum R57 on a seed culture medium for shake culture to obtain a eupenicillium javanicum R57 seed suspension; picking 2-3 bacillus amyloliquefaciens by an inoculating loop, and placing the bacillus amyloliquefaciens on a nutrient broth culture medium for shake culture to obtain bacillus amyloliquefaciens seed suspension; picking Burkholderia gladioli Fse32 by using an inoculating loop, and placing the Fse32 on a seed culture medium for culturing to obtain a Burkholderia gladioli Fse32 seed suspension;
(2) Mixing the 4 seed suspensions according to the volume ratio of 1:1:1, and placing the mixture on a fermentation medium for fermentation culture to obtain a fermented secondary seed liquid, wherein the nitrogen source of the fermentation medium is wheat bran; the concentration of the nitrogen source in the fermentation medium is 9.0 g/L;
(3) Performing amplification fermentation culture on the obtained fermented secondary seed liquid according to the volume ratio of 10% v/v, wherein the carbon source of an amplification fermentation culture medium is rice straw powder, wheat straw powder, corn straw powder or vinasse, the nitrogen source of the amplification fermentation culture medium is wheat bran, and the water content of the culture medium is 70%; amplifying fermentation culture to obtain a compound microbial fermentation bacterial fertilizer;
Wherein, in the step (1), the chaetomium globosum DX-THS3, the eupenicillium javanicum R57 and the Burkholderia gladiolus Fse32 are all preserved in China center for type culture collection, and the preservation numbers are CCTCC NO: M2016005, CCTCC NO: M2019831 and CCTCC NO: M2014672 respectively; the bacillus amyloliquefaciens is purchased from North Nalozenges for commercial strains, and is numbered: BNCC 223006.
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CN109574719A (en) * 2018-12-26 2019-04-05 湖北大学 A kind of multifunctional composite microbe bacterial manure and its application
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