CN115137684A - Use of willow herb and/or extract thereof in preparation of bacteriostatic product and composition thereof - Google Patents
Use of willow herb and/or extract thereof in preparation of bacteriostatic product and composition thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses application of willow herb and/or an extract thereof in preparation of a bacteriostatic product. The invention also provides a bacteriostatic composition, the active ingredients of which comprise willow herb and/or extracts thereof; further, cortex Phellodendri and/or its extract can be added. The bacteriostatic composition has good bacteriostatic effect, and can be used for preparing bacteriostatic related products such as non-washing hand gels.
Description
Technical Field
The invention belongs to the technical field of daily chemical articles, and particularly relates to an application of willow herb and/or willow herb extract in preparation of antibacterial products and a composition thereof.
Background
With the improvement of living standard of people and the enhancement of health consciousness, the demand of residents in China for the hand sanitizer is continuously increased. At present, the no-wash gel has the advantage of convenience, so that the no-wash gel becomes a necessary product for daily life of people, and the anti-bacteria hand-wash gel is gradually accepted by consumers. In order to improve the cleaning power and the oil removal capability, the formula of the antibacterial hand washing gel in the prior art usually selects components such as a surfactant, a foaming agent, a soap base and the like with strong alkalinity as main raw materials, and is matched with chemical reagents such as a bactericide capable of killing bacteria and fungi, the hand washing gel of the formula has certain detergency, but has strong irritation, the skin of a human body is weakly acidic, the residue of the hand washing gel of the chemical formula is strong, the skin can be irritated after long-time use, more seriously, the sebum membrane of the skin can be damaged, and once the sebum membrane is lost, the skin is easily damaged by the outside. In daily life, hand injuries often occur, and hand wound infection is likely to be caused if common hand-free gel is used in the hand cleaning process.
Therefore, consumers expect a no-wash gel which can inhibit bacteria, has faint scent and no toxic or side effect, and has better antibacterial effect, prevents bacterial viruses from invading bodies and reduces the occurrence of diseases in the daily cleaning process.
Disclosure of Invention
In order to solve the problems, the invention provides the application of willow herb and/or the extract thereof in preparing bacteriostatic products and the composition thereof.
In order to achieve the purpose, the invention provides the application of willow herb and/or the extract thereof in preparing bacteriostatic products.
Such products include, but are not limited to, pharmaceuticals, commodity products, and the like; such daily necessities include, but are not limited to, hand sanitizer, hand gel, leave-on gel, body wash, soap, cosmetics, and the like.
Further, the bacteria are selected from gram-positive bacteria, gram-negative bacteria and candida albicans;
further, the bacteria are selected from Escherichia coli, staphylococcus aureus, and Candida albicans.
The willow herb and/or the willow herb extract are/is used as an active component of a bacteriostatic product, and the active component is a part which plays a therapeutic role in the product.
The invention provides an application of willow herb and/or willow herb extract and golden cypress, in particular to an application of willow herb and/or willow herb extract and golden cypress, golden cypress extract or golden cypress extract in preparing antibacterial products.
The invention also provides a bacteriostatic composition, and the active component of the bacteriostatic composition contains willow herb and/or extract thereof.
Further, the mass volume ratio of the willow herb and/or the willow herb extract is 0.1-0.4: 1.
further, the traditional Chinese medicine composition also contains phellodendron and/or extract thereof;
further, the mass volume ratio of the phellodendron amurense and/or the extract thereof is 0.5-2: 1.
the unit of the mass-to-volume ratio is g: ml.
In a specific embodiment of the present invention, the preparation method of the phellodendron amurense extract comprises the following steps: decocting the raw materials with water, concentrating the obtained extractive solution, settling, and drying.
In a specific embodiment of the present invention, the preparation method of the willow herb extract comprises the following steps: extracting herba Epilobii Angustifoii with water, precipitating with ethanol or extracting with ethanol, concentrating, and drying to obtain dry extract powder.
The solvent used in the preparation method of the invention is determined according to the amount of the medicinal materials as long as the extraction requirements can be met, and is not limited specifically. The extractive solution obtained by the above preparation method can be filtered and dried to obtain dry powder, i.e. Chinese medicinal extract.
The invention also provides a preparation method of the antibacterial traditional Chinese medicine composition, which comprises the following steps: (1) selecting each component of the bacteriostatic traditional Chinese medicine composition; (2) grinding; and (3) mixing.
In the preparation method, "(2) grinding; (3) The mixing sequence can be adjusted, and can be (2) first and then (3), or (3) first and then (2), that is, the Chinese medicinal extracts can be respectively ground and then mixed, or the Chinese medicinal extracts can be mixed and then ground.
The invention also provides a no-wash hand gel, wherein each 100g of the no-wash hand gel comprises: 0.1-15 g of the antibacterial composition, 0.1-3.0 g of gel matrix, 30-80 g of ethanol, 1-5 g of glycerol and 1-70 g of water.
Further, each 100g of hand wash gel comprises: 5-15 g of the bacteriostatic traditional Chinese medicine composition, 0.5-1.5 g of gel matrix, 30-80 g of ethanol, 2-4 g of glycerol and the balance of water;
the gel matrix is one or more selected from carbomer 940, carbomer U21, carbomer U20, tragacanth, sodium alginate and polyvinylpyrrolidone; in a specific embodiment of the invention, the gel matrix is carbomer 940.
Further, the hand-washing-free gel also comprises 0.01-5 g of essence.
According to the actual needs, other raw materials such as preservatives (cason and ECOCIDE ITH2 preservatives), essence, pigment and the like can be added, and the other raw materials are added to basically realize the respective functions, and usually do not influence the basic performance of the invention. And can be carried out by those skilled in the art without inventive effort.
Has the advantages that:
(1) The invention provides a product for preparing bacteriostatic by using willow herb and/or extract thereof, and particularly has better bacteriostatic effect on staphylococcus aureus, escherichia coli, candida albicans and the like after being combined with phellodendron extract.
(2) The bacteriostatic traditional Chinese medicine composition provided by the invention comprises the willow herb extract and/or the golden cypress extract, bacteriostatic components are traditional Chinese medicine extracts, and the traditional Chinese medicine composition can clean skin after being prepared into hand-washing-free gel, and is effective in bacteriostasis, small in irritation, safe and healthy.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It is to be noted that the following processes, if not described in particular detail, are all realizable or understandable by those skilled in the art with reference to the prior art, and those not indicated in the examples are carried out according to conventional conditions or conditions suggested by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1 preparation of extract
The preparation method of the phellodendron extract and the ruta extract comprises the following steps: putting the crushed phellodendron raw material into an extraction tank, decocting with water for 3 times, wherein the water discharge ratio is 4.
The preparation method of the willow herb extract comprises the following steps: (1) Soaking the selected medicinal materials in water, washing, pulverizing, parching, and extracting; (2) Extracting the fried medicinal materials with water, performing triple-effect concentration, precipitating the obtained concentrated solution with ethanol, concentrating, and drying to obtain dry extract powder;
or preparing the willow herb extract by adopting the following preparation method: (2) Extracting the fried medicinal materials with ethanol, vacuum concentrating the extractive solution, and spray drying to obtain dry extract powder.
The preparation method of the soapberry extract comprises the following steps: cleaning raw materials, drying, pulverizing, extracting with water under reflux, filtering, concentrating, spray drying, inspecting, and packaging.
The preparation method of Althaea officinalis extract comprises the following steps: cleaning raw materials, extracting, concentrating, purifying, drying extract, pulverizing, inspecting, and packaging.
Example 2
Bacteriostatic experiment I
1. Pretreatment of bacteriostatic traditional Chinese medicine composition
Grinding the raw material extract powder to obtain extract fine powder.
2. Preparation of test article
Respectively taking 1g of cortex Phellodendri extract, herba Epilobii Angustifoii extract, fructus Sapindi Mukouossi extract, althaea officinalis extract, and Ruta graveolens extract, adding appropriate amount of deionized water, and dissolving completely to obtain test sample with the same concentration or dosage.
3. Preparation of suspension: respectively culturing Staphylococcus aureus, escherichia coli, and Candida albicans in biochemical incubator at 37 deg.C for 1d, and adding 4 ml broth culture medium. Transferring the bacterial suspension into a 10 ml centrifugal tube, shaking for 4h in a shaking table to obtain the bacterial suspension, and standing for 10min.
4. Preparing the bacteriostatic tablet: a sterile dried filter paper sheet (diameter 5 mm) was soaked in the sample specimen. And (4) taking the sterile dry filter paper sheet, soaking the sterile dry filter paper sheet in deionized water, and preparing a negative control sample sheet.
5. Experimental method
1) Bacteriostatic ring experiment I
Respectively and uniformly coating 200 mu L of bacterial suspension of staphylococcus aureus, escherichia coli and candida albicans on a solid plate by using a coating rod, and airing. Dividing the plate into 5 areas and marking (4 test sample pieces, 1 negative control sample piece), spreading medicated filter paper on the marked area of the plate with forceps, inverting the plate, culturing in a biochemical incubator at 37 deg.C, observing bacteriostatic effect every day, measuring bacteriostatic zone, obtaining bacteriostatic effect of different Chinese medicinal extracts according to the bacteriostatic zone result, and screening 1-2 Chinese medicinal extracts with good bacteriostatic effect as adjuvant of cortex Phellodendri. The test was repeated 3 times, and the data in the table are all mean values.
TABLE 1 diameter of zone of inhibition (cm) of five single Chinese medicinal extracts to different bacteria
Bacteria | Huang Bai | Willow herb | Althaea officinalis | Ruta graveolens (L.) Merr | Soapberry |
Staphylococcus aureus | 2.27 | 1.05 | 0.70 | 0.80 | 0.75 |
Candida albicans | 1.47 | 0.77 | 1.13 | 0.78 | 1.55 |
Escherichia coli | 0.75 | 0.73 | - | - | - |
Note: "-" indicates no zone of inhibition.
2) Inhibition of bacteria ring experiment 2
Respectively and uniformly coating 200 mu L of bacterial suspension of staphylococcus aureus, escherichia coli and candida albicans on a solid plate by using a coating rod, and airing. Dividing the plate into 5 areas and marking, spreading medicated filter paper on the marked area of the plate with forceps, inverting the plate, culturing in a biochemical incubator at 37 deg.C, observing bacteriostatic effect every day, and determining bacteriostatic circle. The experiment was repeated 3 times.
The experiment sets 5 different concentrations, aims to screen the optimal bacteriostatic concentration of the phellodendron and the willow orchid, and screens the proper concentration for the later two-medicine compatibility experiment.
TABLE 2 diameter of zone of inhibition (cm) of phellodendron extract
Note: "-" indicates the diameter of the zone of inhibition <0.7cm
3) Research on compatibility and proportion of medicinal material extracts
According to the compatibility principle of the traditional Chinese medicine and the bacteriostatic ability of the extracts of the four traditional Chinese medicines to different bacterial colonies, the first traditional Chinese medicine of willow herb and golden cypress are selected to be combined for bacteriostatic experiments by combining earlier-stage experiments. Meanwhile, a single traditional Chinese medicine extract is arranged for carrying out an antibacterial test (the test is carried out under the same concentration).
All experiments were performed in 3 replicates and the data were statistically processed using SPSS21.0 software and expressed as mean. + -. Standard deviation (X. + -. SD). The optimal proportion is obtained according to the experimental result.
TABLE 3 test results of bacteriostasis of extracts with different concentrations in Chinese herbal compound
Note: "-" indicates the diameter of the zone of inhibition <0.7cm
And (4) analyzing results: as can be seen from the above table, in the mixture of the traditional Chinese medicine extracts, when the mass concentrations of the phellodendron amurense and the willow herb are respectively 0.125g/mL and 1g/mL, the in vitro antibacterial activity of the phellodendron amurense and the willow herb is optimal. The mixed liquor of the phellodendron and the willow herb extract has better in-vitro antibacterial activity on escherichia coli than that of the phellodendron extract liquor with the same concentration, which shows that the willow herb can cooperate with the phellodendron to increase the capacity of inhibiting the escherichia coli.
The mass concentrations of the phellodendron and the willow herb in the traditional Chinese medicine extract mixture are respectively 0.25g/mL and 2g/mL, and the external bacteriostatic activity of the phellodendron and the willow herb in the traditional Chinese medicine extract mixture is optimal for staphylococcus aureus. But the in-vitro bacteriostatic activity of the mixed liquid medicine of the phellodendron amurense and the willow herb extract on staphylococcus aureus is lower than that of the phellodendron amurense extract liquid medicine with the same concentration.
The mass concentrations of the phellodendron and the willow herb in the traditional Chinese medicine extract mixture are respectively 0.125g/mL and 1g/mL, and the external bacteriostatic activity of the traditional Chinese medicine extract mixture is optimal for candida albicans. The in-vitro bacteriostatic activity of the mixed liquor of the phellodendron amurense and the willow herb extract is superior to that of the phellodendron amurense extract liquor with the same concentration, which shows that the willow herb can cooperate with the phellodendron amurense to increase the capacity of the phellodendron amurense to inhibit candida albicans.
4) MIC Experimental procedures and results
Taking 12 sterile test tubes, numbering in sequence, adding 1ml of sterile culture solution into each tube of No. 1-10, adding 1ml of sample into No. 1 tube (cortex Phellodendri and willow sample are each subjected to 3 groups of parallel tests), mixing uniformly, sucking 1ml of sample, adding into No. 2 tube, diluting in sequence according to the steps, and sucking 1ml of sample from the tube when the number 10 tube is reached and discarding. Tube 11 was growth control, 1mL of sterile medium was added, and then 0.2mL of test bacterial suspension at a concentration of 106CFU/mL was added to each tube 1-11. In addition, no. 12 pure medium was used as a blank. Performing shake culture at 37 deg.C for 24 hr, parallelly collecting 100 μ L suspension, dyeing with TTC in 96-well plate for 3 times, and observing the plate to obtain Minimum Inhibitory Concentration (MIC) with maximum concentration of 2-3 plate with red color.
TABLE 5 MIC of Chinese medicinal extract for inhibiting Staphylococcus aureus
Note: "-" indicates TTC staining as red; HB-Phellodendri cortex, LL-willow orchid, the same below.
The experimental result shows that the MIC value of the phellodendron extract to the staphylococcus aureus is 2.02mg/mL, and the MIC value of the willow herb to the staphylococcus aureus is 4.03mg/mL.
The MIC value of cortex Phellodendri extract to Escherichia coli is 4.03mg/mL, and the MIC value of willow herb to Escherichia coli is 4.03mg/mL.
The MIC value of the phellodendron extract to Candida albicans is 16.13mg/mL, and the MIC value of willow herb to Candida albicans is 2.02mg/mL.
TABLE 6 MIC for combination of Chinese medicinal extracts 1 for bacteriostasis
Note: "-" indicates that TTC staining was red.
The experimental results show that the growth control group is red, the blank control is culture solution, and no color change occurs.
In the traditional Chinese medicine extract composition, the MIC value of 0.125g/mL of the phellodendron extract and 1g/mL of the willow herb extract to staphylococcus aureus is 1.10mg/mL and is less than the MIC value of 2.02mg/mL of phellodendron and 4.03mg/mL of willow herb to staphylococcus aureus;
the MIC value of the compound to the escherichia coli is 2.20mg/mL, which is less than the MIC values of phellodendron (4.03 mg/mL) and willow herb (4.03 mg/mL) to the escherichia coli;
the MIC value for Candida albicans was 0.55mg/mL, which was less than the MIC values for Phellodendri cortex (16.13 mg/mL) and willow herb (2.02 mg/mL) for Candida albicans.
TABLE 7 MIC of combination of Chinese medicinal extracts 2 for bacteriostasis
Note: "-" indicates that TTC staining was red.
The experimental results show that the growth control group is red, the blank control is culture solution, and no color change occurs.
In the traditional Chinese medicine extract composition, the MIC value of 0.25g/mL of the phellodendron extract and 2g/mL of the willow herb extract to staphylococcus aureus is 1.10mg/mL and is less than the MIC value of the phellodendron (2.20 mg/mL) and the willow herb (4.03 mg/mL) to staphylococcus aureus;
the MIC value to the Escherichia coli is 4.39mg/mL, which is larger than the MIC values of phellodendron (4.03 mg/mL) and willow herb (4.03 mg/mL) to the Escherichia coli;
the MIC for Candida albicans was 1.10mg/mL, which was less than the MIC for Phellodendri cortex (16.13 mg/mL) and willow herb to Candida albicans (2.02 mg/mL).
As shown in tables 6 and 7, the MIC values of the combination of the Chinese medicinal extracts are the minimum against Staphylococcus aureus, escherichia coli and Candida albicans when the amount of the phellodendron extract is 0.125g/mL and the amount of the willow herb extract is 1 g/mL.
Example 3 bacteriostatic no-wash hand gel
Every 100g of hand washing gel comprises 5-15 g of willow herb extract and phellodendron extract, 0.5-1.5 g of gel matrix, 30-80 g of ethanol, 2-4 g of glycerol, 0.01-5 g of essence and the balance of water.
Claims (10)
1. Use of willow herb and/or its extract in preparing bacteriostatic products.
2. Use according to claim 1, characterized in that said bacteria are selected from gram-positive bacteria, gram-negative bacteria, candida albicans;
further, the bacteria are selected from Escherichia coli, staphylococcus aureus, and Candida albicans.
3. Use of willow herb and/or its extract and phellodendron bark in preparing bacteriostatic product.
4. A bacteriostatic composition is characterized in that the active component of the bacteriostatic composition contains willow herb and/or an extract thereof.
5. The bacteriostatic traditional Chinese medicine composition according to claim 4, wherein the mass-volume ratio of the willow herb and/or the extract thereof is 0.1-0.4: 1;
further, the preparation method of the willow herb extract comprises the following steps: extracting herba Epilobii Angustifoii with water, precipitating with ethanol or extracting with ethanol, concentrating, and drying to obtain dry extract powder.
6. The bacteriostatic traditional Chinese medicine composition according to claim 4 or 5, which further comprises phellodendron amurense and/or extract thereof;
further, the mass volume ratio of the phellodendron amurense and/or the extract thereof is 0.5-2: 1.
7. the bacteriostatic traditional Chinese medicine composition according to claim 6, wherein the preparation method of the phellodendron extract comprises the following steps: decocting the raw materials with water, concentrating the obtained extractive solution, settling, and drying.
8. A preparation method of a bacteriostatic traditional Chinese medicine composition is characterized by comprising the following steps: (1) Selecting each component of the bacteriostatic traditional Chinese medicine composition according to any one of claims 4 to 7; (2) grinding; and (3) mixing.
9. A leave-on hand gel, comprising per 100g of hand wash gel: 0.1-15 g of bacteriostatic composition according to any one of claims 5-7, 0.1-3.0 g of gel matrix, 30-80 g of ethanol, 1-5 g of glycerol and 1-70 g of water.
10. The leave-in hand gel of claim 9, wherein each 100g of hand wash gel comprises: 5-15 g of the bacteriostatic traditional Chinese medicine composition of any one of claims 5-7, 0.5-1.5 g of gel matrix, 30-80 g of ethanol, 2-4 g of glycerol and the balance of water;
furthermore, the hand-free gel also comprises 0.01-5 g of essence.
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