CN112156042B - Manufacturing process of shampoo - Google Patents
Manufacturing process of shampoo Download PDFInfo
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Abstract
The invention discloses a manufacturing process of shampoo, which belongs to the technical field of bath supplies, wherein the formula of the shampoo contains anti-dandruff and itching-relieving components, and the anti-dandruff and itching-relieving components are dictamnine and/or matrine.
Description
Technical Field
The invention belongs to the technical field of bath supplies, and particularly relates to a manufacturing process of shampoo.
Background
Malassezia yeast-like fungus, a conditional pathogenic bacterium, is a normal parasite on the skin of warm-blooded animals and humans, mainly grows in the stratum corneum of human skin, and has lipophilicity. In recent years, excessive proliferation of malassezia on the scalp has been considered to be an important cause of seborrheic dermatitis of the scalp. Malassezia furfur, malassezia globosa, and Malassezia syzygium exist on scalp. At present, western medicines aiming at malassezia are mainly antifungal preparations, but have large adverse reaction in treatment and are easy to generate drug resistance, so that the task of searching for an antibacterial drug which has safe action, satisfactory curative effect and reasonable price is still arduous.
The screening research of antifungal traditional Chinese medicines has been carried out for many years, and compared with the traditional western antifungal medicines, the antifungal traditional Chinese medicines have the advantages of small adverse reaction, low price, less occurrence of drug resistance and the like, wherein the cortex dictamni and the radix sophorae flavescentis can clear heat, promote diuresis, relieve itching, enhance the functions of inhibiting bacteria and relieving itching, and dictamnine in the cortex dictamni and matrine in the radix sophorae flavescentis have better inhibiting effect on malassezia.
At present, organic solvents are mostly adopted for extracting dictamnine and matrine for multiple times, the time consumption is long, the steps are multiple, and the exploration of an efficient extraction method has important significance.
Disclosure of Invention
The invention aims to provide the method for extracting the Chinese medicinal anti-dandruff and anti-itching components, which has the advantages of high extraction amount and short extraction time and can improve the extraction effect of the Chinese medicinal anti-dandruff and anti-itching components.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a method for extracting Chinese medicinal components with dandruff removing and antipruritic effects comprises:
s1, treating traditional Chinese medicine powder in a pretreatment liquid to obtain pretreated traditional Chinese medicine powder, wherein the pretreatment liquid is an alkali liquor containing sodium sulfite and 1-amino-3-methyl urea; the alkali liquor containing sodium sulfite and 1-amino-3-methyl urea improves the extraction amount of alkaloid and reduces the extraction time, probably because the pretreatment liquor dissolves out the water-soluble and alkali-soluble components of the traditional Chinese medicine powder, the sodium sulfite and the 1-amino-3-methyl urea destroy the cross-linking effect in cells, the effective contact area of the extracting solution is improved, more alkaloid is exposed on the surface area, and thus better extraction effect is obtained in the extraction process.
S2, pretreating the traditional Chinese medicine powder, and extracting in an extracting solution to obtain an extracting crude solution of the dandruff-removing and itching-relieving component;
and S3, carrying out suction filtration, reduced pressure concentration and solvent redissolution on the extracted crude liquid of the dandruff-removing and itching-relieving component, and then separating and purifying by using cation exchange resin to obtain the dandruff-removing and itching-relieving component.
Preferably, drying and pulverizing Chinese medicinal materials, sieving with 50-100 mesh sieve, adding Chinese medicinal powder into pretreatment solution, treating at 60-80 deg.C for 0.5-1h, centrifuging, discarding supernatant, washing with distilled water, and oven drying at 30-50 deg.C to obtain pretreatment Chinese medicinal powder; the pretreatment solution is an alkali solution containing sodium sulfite and 1-amino-3-methyl urea, and the addition amount of the traditional Chinese medicine powder is 6-18wt% of the pretreatment solution.
More preferably, the Chinese medicine is cortex Dictamni and/or radix Sophorae Flavescentis.
More preferably, the alkali solution is 0.5-2.5wt% of sodium hydroxide solution, the sodium sulfite is added in an amount of 0.4-1.6wt% of the sodium hydroxide solution, and the 1-amino-3-methylurea is added in an amount of 0.3-0.9wt% of the sodium hydroxide solution.
More preferably, the dandruff removing and itching relieving component is dictamnine and/or matrine. The dictamnine and/or matrine has inhibiting effect on malassezia, and can improve dandruff removing and itching relieving effects.
Preferably, the pre-treated traditional Chinese medicine powder is added into an ethanol solution, extracted for 0.5-2h at the temperature of 50-70 ℃, repeatedly extracted for more than two times, the extracting solution is combined to obtain the crude extract of the anti-dandruff and anti-itching ingredient, the crude extract is filtered, decompressed and concentrated to be dry, and the anti-dandruff and anti-itching ingredient is obtained by separating and purifying the crude extract by cation exchange resin after being dissolved by methanol; the mass fraction of the ethanol solution is 70-90wt%, the addition amount of the traditional Chinese medicine powder is 2-5wt% of the ethanol solution, and the dosage of the methanol solution is 10-20wt% of the ethanol solution.
The invention discloses an application of sodium sulfite and 1-amino-3-methyl urea, which is at least one of the following 1) -2):
1) Improving the extraction amount of the Chinese medicinal components for removing dandruff and relieving itching;
2) Reducing the extraction time of the components for removing dandruff and relieving itching.
The invention aims to provide a formula of shampoo which has the effects of removing dandruff, relieving itching, better inhibiting effect on malassezia, qualified quality and good stability.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a formula of shampoo comprises: ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide, silicone emulsion, amino silicone oil, pearling agent, dandruff-removing and itching-relieving component, chelating agent, antiseptic, reinforcing agent, essence, pH regulator, and water. The dandruff-removing and itching-relieving component is dictamnine and/or matrine. The pearling agent is ethylene glycol monostearate. The chelating agent is disodium EDTA. The antiseptic is Kathon. The enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium. The essence is flower fragrance and fruit fragrance essence. The pH regulator is citric acid and sodium citrate.
Preferably, the mass ratio of the dictamnine to the matrine is 1: mixing at a ratio of 0.2-5.
Preferably, the mass ratio of the 2-amino-4-carbamyl butyric acid to the acesulfame potassium in the enhancer is 1: mixing at a ratio of 0.3-2. When the 2-amino-4-carbamyl butyric acid and the acesulfame potassium exist together with the dictamnine and/or the matrine, the 2-amino-4-carbamyl butyric acid and the acesulfame potassium have the effect of improving the inhibiting effect of the dictamnine and/or the matrine on the malassezia, and the 2-amino-4-carbamyl butyric acid or the acesulfame potassium cannot improve the inhibiting effect on the malassezia when being used alone.
Preferably, the pH adjusting agent is citric acid and sodium citrate in a molar ratio of 1: mixing at a ratio of 0.5-2.
Preferably, a formulation for a shampoo comprises: the composition comprises, by mass, 20-30 parts of lauryl alcohol polyether ammonium sulfate, 2-4 parts of cocamidopropyl betaine, 15-25 parts of lauryl alcohol ammonium sulfate, 1-3 parts of alkyl glucoside, 0.1-1 part of cocamide, 2-5 parts of silicone emulsion, 2-5 parts of amino silicone oil, 1-3 parts of pearling agent, 2-5 parts of dandruff-removing and itching-relieving components, 0.05-0.1 part of chelating agent, 0.05-0.4 part of preservative, 0.2-1.2 parts of reinforcing agent, 0.3-0.9 part of essence, 0.3-3 parts of pH regulator and 50-70 parts of water.
The invention discloses 2-amino-4-carbamyl butyric acid and acesulfame potassium which have the effect of improving the inhibition effect of dictamnine and/or matrine on malassezia.
The invention aims to provide a manufacturing process of shampoo which has the advantages of dandruff removal, itching relieving, good malassezia inhibition effect, qualified quality and good stability.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a manufacturing process of shampoo comprises the following steps:
s1, heating water to 80-90 ℃, sequentially adding ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 55-65 ℃, adding emulsified silicone oil, amino silicone oil, an anti-dandruff and itching-relieving component and a pearling agent, and stirring and mixing uniformly;
and S3, cooling the water to 40-50 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
The invention adopts the alkali liquor containing sodium sulfite and 1-amino-3-methyl urea to extract the dittany bark or the matrine, thereby having the following beneficial effects: the extraction amount of dictamnine in the cortex dictamni is improved, and the extraction effect is good; the extraction amount of matrine in the matrine is improved, the extraction effect is good, and compared with the pretreatment without adding sodium sulfite and 1-amino-3-methyl urea alkali liquor, the total extraction amount of dictamnine twice is improved by 94.31%, and the total extraction amount of matrine twice is improved by 108.19%. According to the invention, the liquid shampoo with a good inhibition effect on malassezia is obtained by adopting the intensifier as the formula of the liquid shampoo, and the dandruff removing and itching relieving effects are good. Therefore, the manufacturing process of the shampoo provided by the invention has the advantages of dandruff removal, itching relieving, good malassezia inhibition effect, qualified quality and good stability.
Drawings
FIG. 1 is a diagram of the amount of the components extracted from the Chinese medicinal composition for removing dandruff and relieving itching;
FIG. 2 is a graph of the amount of the Chinese medicinal composition for removing dandruff and relieving itching during different extraction times;
FIG. 3 is a 48hMIC value diagram of Chinese medicinal dandruff removing and itching relieving component on malassezia;
FIG. 4 is a 72hMIC value diagram of Chinese medicinal dandruff removing and itching relieving component on Malassezia.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
the dictamnine in the embodiment 1 and the matrine in the embodiment 2 are identified by thin-layer chromatography, a silica gel G thin-layer plate is adopted, a developing agent is toluene-ethyl acetate-methanol-water (2. The color developing agent is potassium bismuth iodide.
The reference substance, dictamnine or matrine, is prepared from China institute for testing pharmaceutical and biological products, by adding methanol to obtain a solution of 0.2mg/mL.
Example 1:
a method for extracting dandruff-removing and itching-relieving components of Chinese medicine,
pretreatment: drying and crushing the dittany bark, sieving with a 50-mesh sieve, adding the dittany bark powder into the pretreatment liquid, treating at the temperature of 70 ℃ for 0.5h, centrifuging, removing a supernatant, washing with distilled water, and drying at the temperature of 40 ℃ to obtain the pretreated dittany bark powder; the pretreatment liquid is 1.5wt% of sodium hydroxide solution, the pretreatment liquid contains sodium sulfite and 1-amino-3-methyl urea, the addition amount of the sodium sulfite is 0.8wt% of the sodium hydroxide solution, the addition amount of the 1-amino-3-methyl urea is 0.6wt% of the sodium hydroxide solution, and the addition amount of the cortex dictamni powder is 12wt% of the pretreatment liquid.
Extraction, separation and purification: adding pretreated cortex Dictamni Radicis powder into ethanol solution, leaching at 60 deg.C for 1 hr, repeating the extraction twice, mixing extractive solutions to obtain crude extract of dandruff-removing and itching-relieving components, vacuum filtering, concentrating under reduced pressure to dry, dissolving in methanol, separating and purifying with cation exchange resin to obtain dictamnine, identifying dictamnine with thin layer chromatography in the eluate, and collecting the required components; the mass fraction of the ethanol solution is 80wt%, the addition amount of the cortex dictamni powder is 4wt% of the ethanol solution, and the use amount of the methanol solution is 12wt% of the ethanol solution.
The cation exchange resin is 732 type strong acid cation exchange resin, national drug group chemical reagent limited.
Cation exchange resin treatment: taking 50g of cation exchange resin, soaking the cation exchange resin in 300mL of deionized water overnight, and washing the cation exchange resin for multiple times until the deionized water is nearly colorless; 250mL of 7% hydrochloric acid solution was added and soaked for 1h with stirring at any time. The solution is poured, washed by deionized water to be nearly neutral, and then 250mL of 2mol/L sodium hydroxide solution is added for soaking for 1h. Washing with deionized water to be neutral, finally adding 250mL of 7% hydrochloric acid solution, soaking for about 2h, washing with deionized water to adjust the pH to 6, and then loading into a column with the column height of 530mm.
Eluting with 15% hydrochloric acid solution at flow rate of 0.7mL/min, volatilizing in water bath, adding methanol for redissolution, filtering with 0.2 μm filter membrane, and removing methanol to obtain dictamnine.
Example 2:
a method for extracting dandruff-removing and itching-relieving components of Chinese medicine,
pretreatment: drying radix Sophorae Flavescentis, pulverizing, sieving with 50 mesh sieve, adding radix Sophorae Flavescentis powder into the pretreatment solution, treating at 70 deg.C for 0.5h, centrifuging, discarding supernatant, washing with distilled water, and oven drying at 40 deg.C to obtain pretreated radix Sophorae Flavescentis powder; the pretreatment liquid is 1.5wt% of sodium hydroxide solution, the pretreatment liquid contains sodium sulfite and 1-amino-3-methyl urea, the addition amount of the sodium sulfite is 0.8wt% of the sodium hydroxide solution, the addition amount of the 1-amino-3-methyl urea is 0.6wt% of the sodium hydroxide solution, and the addition amount of the sophora flavescens powder is 12wt% of the pretreatment liquid.
Extraction, separation and purification: adding pretreated radix Sophorae Flavescentis powder into ethanol solution, leaching at 60 deg.C for 1 hr, repeatedly extracting twice, mixing extractive solutions to obtain crude extract of dandruff-removing and itching-relieving components, vacuum filtering, concentrating under reduced pressure to dry, dissolving in methanol, separating and purifying with cation exchange resin to obtain matrine, identifying matrine with thin layer chromatography in the eluate, and collecting the required components; the mass fraction of the ethanol solution is 80wt%, the addition amount of the radix sophorae flavescentis powder is 4wt% of the ethanol solution, and the dosage of the methanol solution is 12wt% of the ethanol solution.
The cation exchange resin is 732 type strong acid cation exchange resin, national drug group chemical reagent limited.
Cation exchange resin treatment: taking 50g of cation exchange resin, soaking the cation exchange resin in 300mL of deionized water overnight, and washing the cation exchange resin for multiple times until the deionized water is nearly colorless; 250mL of 7% hydrochloric acid solution was added and soaked for 1h with stirring. The solution is poured, washed by deionized water to be nearly neutral, and then 250mL of 2mol/L sodium hydroxide solution is added for soaking for 1h. Washing with deionized water to be neutral, finally adding 250mL of 7% hydrochloric acid solution, soaking for about 2h, washing with deionized water to adjust the pH to 6, and then loading into a column with the column height of 530mm.
Eluting with 15% hydrochloric acid solution at flow rate of 0.7mL/min, volatilizing in water bath, adding methanol for redissolution, filtering with 0.2 μm filter membrane, and removing methanol to obtain matrine.
Example 3:
a formula of shampoo comprises the following components in parts by mass,
25 parts of ammonium laureth sulfate, 3 parts of cocamidopropyl betaine, 20 parts of ammonium laureth sulfate, 2 parts of alkyl glucoside, 0.5 part of cocamide, 4 parts of emulsified silicone oil, 3 parts of amino silicone oil, 2 parts of a pearling agent, 4 parts of an anti-dandruff and anti-itching component, 0.05 part of a chelating agent, 0.2 part of a preservative, 0.5 part of a reinforcing agent, 0.6 part of essence, 1.2 parts of a pH regulator and 60 parts of water.
The anti-dandruff and anti-itching ingredient is dictamnine obtained in example 1. The pearling agent is ethylene glycol monostearate. The chelating agent is disodium EDTA. The antiseptic is Kathon. The enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium. The essence is flower fragrance and fruit fragrance essence. The pH regulator is citric acid and sodium citrate.
The mass ratio of the 2-amino-4-carbamyl butyric acid to the acesulfame potassium in the reinforcing agent is 1:1 in the ratio of 1.
The pH regulator is citric acid and sodium citrate in a molar ratio of 1:1 in the ratio of 1.
A process for preparing shampoo liquid comprises preparing a liquid shampoo,
s1, heating water to 90 ℃, sequentially adding ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 60 ℃, adding emulsified silicone oil, amino silicone oil, the anti-dandruff and itching-relieving component and the pearling agent, and uniformly stirring and mixing;
and S3, cooling the water to 40 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
Example 4:
a formula of shampoo comprises the following components by mass,
25 parts of laureth ammonium sulfate, 3 parts of cocamidopropyl betaine, 20 parts of laureth ammonium sulfate, 2 parts of alkyl glucoside, 0.5 part of cocamide, 4 parts of emulsified silicone oil, 3 parts of amino silicone oil, 2 parts of pearling agent, 4 parts of dandruff-removing and itching-relieving component, 0.05 part of chelating agent, 0.2 part of preservative, 0.5 part of reinforcing agent, 0.6 part of essence, 1.2 parts of pH regulator and 60 parts of water.
The anti-dandruff and antipruritic component is matrine obtained in example 2. The pearling agent is ethylene glycol monostearate. The chelating agent is disodium EDTA. The antiseptic is Kathon. The enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium. The essence is flower-fragrance fruit-fragrance essence. The pH regulator is citric acid and sodium citrate.
The mass ratio of the 2-amino-4-carbamyl butyric acid to the acesulfame potassium in the enhancer is 1:1, and mixing.
The pH regulator is citric acid and sodium citrate in a molar ratio of 1:1, and mixing.
A process for preparing shampoo liquid comprises preparing a liquid shampoo,
s1, heating water to 90 ℃, sequentially adding ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 60 ℃, adding emulsified silicone oil, amino silicone oil, the anti-dandruff and itching-relieving component and the pearling agent, and uniformly stirring and mixing;
and S3, cooling the water to 40 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
Example 5:
a formula of shampoo comprises the following components in parts by mass,
25 parts of laureth ammonium sulfate, 3 parts of cocamidopropyl betaine, 20 parts of laureth ammonium sulfate, 2 parts of alkyl glucoside, 0.5 part of cocamide, 4 parts of emulsified silicone oil, 3 parts of amino silicone oil, 2 parts of pearling agent, 5 parts of dandruff-removing and itching-relieving component, 0.05 part of chelating agent, 0.2 part of preservative, 0.5 part of reinforcing agent, 0.6 part of essence, 1.2 parts of pH regulator and 60 parts of water.
The anti-dandruff and anti-itching components are dictamnine obtained in example 1 and matrine obtained in example 2, and the mass ratio of the dictamnine to the matrine is 1:1 in the ratio of 1. The pearling agent is ethylene glycol monostearate. The chelating agent is disodium EDTA. The antiseptic is Kathon. The enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium. The essence is flower fragrance and fruit fragrance essence. The pH regulator is citric acid and sodium citrate.
The mass ratio of the 2-amino-4-carbamyl butyric acid to the acesulfame potassium in the enhancer is 1:1 in the ratio of 1.
The pH regulator is prepared from citric acid and sodium citrate in a molar ratio of 1:1, and mixing.
A process for preparing shampoo liquid comprises preparing a liquid shampoo,
s1, heating water to 90 ℃, sequentially adding ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 60 ℃, adding emulsified silicone oil, amino silicone oil, an anti-dandruff and itching-relieving component and a pearling agent, and stirring and mixing uniformly;
and S3, cooling the water to 40 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
Example 6:
a formula of shampoo comprises the following components by mass,
25 parts of laureth ammonium sulfate, 3 parts of cocamidopropyl betaine, 20 parts of laureth ammonium sulfate, 2 parts of alkyl glucoside, 0.5 part of cocamide, 4 parts of emulsified silicone oil, 3 parts of amino silicone oil, 2 parts of pearling agent, 5 parts of dandruff-removing and itching-relieving component, 0.05 part of chelating agent, 0.2 part of preservative, 0.8 part of reinforcing agent, 0.6 part of essence, 1.2 parts of pH regulator and 60 parts of water.
The anti-dandruff and anti-itching components are dictamnine obtained in example 1 and matrine obtained in example 2, and the mass ratio of the dictamnine to the matrine is 1:1 in the ratio of 1. The pearling agent is ethylene glycol monostearate. The chelating agent is disodium EDTA. The antiseptic is Kathon. The enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium. The essence is flower-fragrance fruit-fragrance essence. The pH regulator is citric acid and sodium citrate.
The mass ratio of the 2-amino-4-carbamyl butyric acid to the acesulfame potassium in the reinforcing agent is 1:1 in the ratio of 1.
The pH regulator is prepared from citric acid and sodium citrate in a molar ratio of 1:1, and mixing.
A process for preparing shampoo liquid comprises preparing a liquid shampoo,
s1, heating water to 90 ℃, sequentially adding ammonium laureth sulfate, cocamidopropyl betaine, ammonium laureth sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 60 ℃, adding emulsified silicone oil, amino silicone oil, the anti-dandruff and itching-relieving component and the pearling agent, and uniformly stirring and mixing;
and S3, cooling the water to 40 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
Comparative example 1:
the comparative example is different from example 1 only in that 1-amino-3-methylurea is not added in the pretreatment and the number of extractions is two.
Comparative example 2:
the comparative example is different from example 1 only in that sodium sulfite was not added in the pretreatment and the number of extractions was two.
Comparative example 3:
the comparative example is different from example 1 only in that sodium sulfite and 1-amino-3-methylurea were not added in the pretreatment, and the number of extractions was two.
Comparative example 4:
the comparative example is different from example 2 only in that 1-amino-3-methylurea is not added in the pretreatment, and the number of extraction times is two.
Comparative example 5:
the comparative example is different from example 2 only in that sodium sulfite was not added in the pretreatment and the number of extractions was two.
Comparative example 6:
the comparative example is different from example 2 only in that sodium sulfite and 1-amino-3-methylurea were not added in the pretreatment, and the number of extractions was two.
Test example 1:
1. extraction effect test of Chinese medicine dandruff-removing and itching-relieving components
The extracted test samples were from examples 1-2 and comparative examples 1-6.
The control sample for HPLC detection is dictamnine or matrine, which is prepared from China institute for pharmaceutical and biological products, and is prepared into 20 μ g/mL with methanol.
The samples obtained in the HPLC test in examples or comparative examples and the control sample were sampled at 10. Mu.L.
The content of dictamnine in the cortex dictamni and matrine in the radix sophorae flavescentis are calculated by an external standard method.
HPLC detection conditions of dictamnine:
the sample was passed through a 0.45 μm filter before injection.
An illite octadecyl-bonded silica gel column (200 mm. Times.4.6 mm,5 μm), column temperature 30 ℃, mobile phase acetonitrile-water (40), flow rate 1.0ml/min, detection wavelength 240nm.
HPLC detection conditions of matrine:
the sample was filtered through a 0.45 μm filter before injection.
kromasi1 C 18 E25744 (200 mm. Times.4.6 mm,5 μm), column temperature 30 ℃, mobile phase triethylamine ethyl-phosphate (22: 0.1% phosphoric acid was adjusted to pH7.6 with triethylamine at a flow rate of 1.0mL/min and a detection wavelength of 220nm.
The extraction amount test is shown in fig. 1, wherein examples 1 and comparative examples 1-3 are obtained by extracting dictamnine from cortex dictamni, and examples 2 and comparative examples 4-6 are obtained by extracting matrine from radix sophorae flavescentis; the primary extraction amount, the secondary extraction amount and the total extraction amount of the dictamnine and the matrine are respectively obtained, wherein in the result of extracting the dictamnine from the cortex dictamni, the extraction amount of the example 1 is the highest and reaches 0.0672mg/g, the extraction amount of the comparative example 1 reaches 0.0474mg/g, the extraction amount of the comparative example 2 reaches 0.0387mg/g, and the extraction amount of the comparative example 3 reaches 0.0386mg/g; compared with the example 1, the 1-amino-3-methyl urea is shown to improve the extraction amount of the dictamnine by using the 1-amino-3-methyl urea; example 1 compared to comparative example 2, shows that the use of sodium sulfite increases the amount of extracted dictamnine; compared with example 3, the use of 1-amino-3-methyl urea and sodium sulfite in example 1 can increase the extraction amount of dictamnine; compared with the comparative example 3, the comparative example 2 shows that the pretreatment of the alkali liquor only containing the 1-amino-3-methyl urea can not improve the extraction amount of the dictamnine; by combining example 1 and comparative examples 1 to 3, it can be seen that the addition of sodium sulfite to the lye increases the amount of extracted dictamnine, and that the use of 1-amino-3-methylurea in combination with sodium sulfite further increases the amount of extracted dictamnine.
In the extraction results of matrine in the sophora flavescens, the extraction amount of the example 2 is the highest and reaches 0.0782mg/g, the extraction amount of the comparative example 4 reaches 0.0514mg/g, the extraction amount of the comparative example 5 reaches 0.0415mg/g, and the extraction amount of the comparative example 6 reaches 0.0412mg/g; compared with example 4, the example 2 shows that the use of 1-amino-3-methyl urea improves the extraction amount of matrine; example 2 compared with comparative example 5, it is shown that the use of sodium sulfite increases the extraction amount of matrine; example 2 compared with example 6, it is shown that the use of 1-amino-3-methyl urea and sodium sulfite can improve the extraction amount of matrine; compared with the comparative example 6, the comparative example 5 shows that the extraction amount of the matrine cannot be increased by only carrying out pretreatment on the 1-amino-3-methyl urea contained in the alkali liquor; by combining example 2 and comparative examples 4-6, it can be obtained that the extraction amount of matrine can be increased by adding sodium sulfite to the alkali solution, and the extraction amount of matrine can be further increased by using 1-amino-3-methylurea and sodium sulfite together.
The change of the secondary extraction amount of the dictamnine and the matrine is basically consistent with the change of the primary extraction amount, and the effect of the 1-amino-3-methyl urea and/or the sodium sulfite in the extraction can be proved.
In the total extraction amount of the dictamnine extracted twice, the total extraction amount of the example 1 reaches 0.1024mg/g, the extraction amount of the comparative example 1 reaches 0.0655mg/g, the extraction amount of the comparative example 3 reaches 0.0527mg/g, the extraction amount of the comparative example 1 is increased by 24.29 percent relative to the extraction amount of the comparative example 3, namely the extraction amount of the dictamnine is increased by 24.29 percent by adding the sodium sulfite; example 1 increased the extraction relative to comparative example 3 by 94.31%, i.e. the addition of sodium sulfite and 1-amino-3-methylurea increased the extraction of dictamnine by 94.31%.
In the total extraction amount of the matrine extracted twice, the total extraction amount of the example 2 reaches 0.1297mg/g, the extraction amount of the comparative example 4 reaches 0.0759mg/g, the extraction amount of the comparative example 6 reaches 0.0623mg/g, and the extraction amount of the comparative example 4 is increased by 21.83 percent relative to the extraction amount of the comparative example 6, namely the extraction amount of the matrine is increased by 21.83 percent by adding sodium sulfite; example 2 the extraction was increased by 108.19% relative to the extraction of comparative example 6, i.e. the addition of sodium sulfite and 1-amino-3-methylurea increased the extraction of matrine by 108.19%.
2. Effect of extraction time on extraction amount
The extraction method adopts the method of example 1 and example 2, and the extraction time is set to 15, 30, 45, 60 and 75min respectively.
The test result is shown in FIG. 2, the extraction amount of dictamnine reaches the maximum value in 45min, the extraction time is prolonged, the extraction amount is not increased basically, the extraction amount of matrine reaches the maximum value in 45min, the extraction time is prolonged, and the extraction amount is not increased basically.
Test example 2:
malassezia external drug sensitivity test
The strain is Malassezia furfur standard strain (CBS 1878) purchased from the mycological institute of dermatosis of Chinese academy of medical sciences.
1. Culture medium
(1) Liquid culture medium of malassezia: maltose 20g/L yeast extract 2g/L glucose 20g/L olive oil 20g/L, peptone 20g/L. Dissolving in distilled water, and autoclaving at 121 deg.C for 15 min.
(2) Olive oil slant solid medium: 40g/L glucose, 14g/L agar, 10g/L peptone, 0.1g/L yeast extract, 2.5g/L glyceryl monostearate, 2mg/L tween-80 mg/L olive oil, dissolving with distilled water, and autoclaving at 121 deg.C for 15 min.
2. Sample adding of dandruff removing and itching relieving components
The dandruff-removing and itching-relieving components are directly diluted into experimental concentrations of 1, 0.8, 0.5, 0.4, 0.38, 0.3, 0.29, 0.25, 0.22, 0.21, 0.2, 0.18, 0.16 and 0.1mg/L by using a malassezia liquid culture medium, and then added into a 96-well round-bottom enzyme label plate, wherein each well is 100 mu L. Meanwhile, a negative control group without adding bacteria and a normal growth control group without adding liquid medicine are arranged, and the negative control group and the normal growth control group are stored at the temperature of minus 20 ℃ for standby, and each group is repeatedly done for 2 times.
3. Bacterial suspension preparation and inoculation
Continuously subculturing the tested bacteria twice in an olive oil culture medium to ensure the purification and the activity of bacterial colonies, after subculturing for 4 days at the temperature of 32 ℃ for 2 times, eluting and purifying the bacterial colonies by using sterile normal saline to prepare bacterial suspension, and adjusting the bacterial suspension to the concentration of a Mach No. 4 turbidimetric tube by using a turbidimeter. The bacterial content of the blood cell counting plate is 1 multiplied by 10 7 CFU/mL, diluting the bacterial liquid 10 times with liquid culture medium, and the bacterial content is 1×10 6 CFU/mL. Melting the prepared microplate at room temperature, adding 100 μ L of bacterial liquid into each well (except negative control group) of the microplate to make the total bacterial concentration 0.5 × 10 6 CFU/m1. The negative control group was added with the same amount of liquid medium.
4. Culture and evaluation of results
And (3) placing the inoculated micropore culture plate in a thermostat with the temperature of 35 ℃ (humidifying) for incubation, and observing results after 48h and 72h respectively, wherein the malassezia is lipophilic, bacterial colonies float on the liquid surface, are yellowish, a thin layer floats on the transparent liquid base surface, the visual observation is very clear, and the data are read by adopting a direct method. The premise of result judgment is that the growth contrast is good, the blank contrast aseptically grows clearly, and the growth of bacteria is limited by other holes along with the increase of the drug concentration gradient. Since there was a tailing phenomenon, the growth in each well was compared with a growth control, and the MIC value was determined as 80% inhibition of growth.
The value greater than the highest concentration is calculated as the last concentration.
The group A is added with 2-amino-4-carbamoylbutyric acid and acesulfame potassium, the proportional relation between the usage amount of the anti-dandruff and anti-itching ingredient and the reinforcing agent is the same as the corresponding proportional relation in the example 6, the anti-dandruff and anti-itching ingredient is divided into a single group and a mixed group, the proportional relation of the mixed group is the same as the example 6, and the proportional relation between the 2-amino-4-carbamoylbutyric acid and the acesulfame potassium is the same as the example 6; B. the component relationship of the group C and the group D is the same as that of the group A, except that 2-amino-4-carbamoylbutyric acid and acesulfame potassium are added, only 2-amino-4-carbamoylbutyric acid is added in the group B, only acesulfame potassium is added in the group C, and 2-amino-4-carbamoylbutyric acid and acesulfame potassium are not added in the group D.
The results of the in vitro drug sensitivity test on malassezia are shown in fig. 3 and 4, the test time of fig. 3 is 48h, and the test time of fig. 4 is 72h. Fig. 3 shows that, in the test results using dictamnine, the MICs of group a are consistent with those of group C and group D, and the MIC of group B is smaller than those of group a, group C and group D, indicating that 2-amino-4-carbamoylbutyric acid and acesulfame potassium can enhance the inhibitory effect of dictamnine on malassezia only under the condition of common use, and that the inhibitory effect of dictamnine on malassezia cannot be enhanced by the single use of 2-amino-4-carbamoylbutyric acid or acesulfame potassium. In the test results of matrine, the effect change of the use of 2-amino-4-carbamyl butyric acid and/or acesulfame potassium on the inhibition of malassezia by matrine is basically consistent with the effect in dictamnine. And the MIC value of the test result of the group A of the mixed group is smaller than that of the dictamnine group and the matrine group, which shows that the effect is better than that of the dictamnine or the matrine when the dictamnine and the matrine are used together respectively.
The change in the test results for 72h was consistent with the change in the test results for 48h, indicating the effect of 2-amino-4-carbamoylbutyric acid and/or acesulfame potassium.
Test example 3:
internal control standard for shampoo quality
The test sample was the shampoo obtained in example 6.
The microbial index test of the prepared shampoo is carried out according to GB/T29679-2013, and the test results are shown as follows.
TABLE 1 microbiological indicators and test results
The microbial indexes of the shampoo are qualified.
The formulated shampoo was subjected to sensory index tests according to GB/T29679-2013, and the test results are shown below.
TABLE 2 sensory index test results
Test results show that the sensory indexes of the shampoo provided by the invention reach the standard and reach the grade of superior products.
Standing at different temperatures for a certain time, and observing stability.
Table 3 stability test results
The shampoo obtained by the invention has good stability.
The above embodiments are merely illustrative, and not restrictive, of the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (6)
1. A method for extracting Chinese medicinal components with dandruff removing and antipruritic effects comprises:
s1, treating traditional Chinese medicine powder in a pretreatment liquid to obtain pretreated traditional Chinese medicine powder, wherein the pretreatment liquid is an alkali liquor containing sodium sulfite and 1-amino-3-methyl urea; the Chinese medicine is cortex Dictamni Radicis and/or radix Sophorae Flavescentis; the alkali liquor is 0.5-2.5wt% of sodium hydroxide solution, the addition amount of the sodium sulfite is 0.4-1.6wt% of the sodium hydroxide solution, and the addition amount of the 1-amino-3-methyl urea is 0.3-0.9wt% of the sodium hydroxide solution; the addition amount of the traditional Chinese medicine powder is 6-18wt% of the pretreatment liquid;
s2, pretreating the traditional Chinese medicine powder, and extracting in an extracting solution to obtain an extracting crude solution of the dandruff-removing and itching-relieving component; the extracting solution is an ethanol solution, the mass fraction of the ethanol solution is 70-90wt%, and the addition amount of the traditional Chinese medicine powder is 2-5wt% of the ethanol solution;
and S3, carrying out suction filtration, reduced pressure concentration and solvent redissolution on the crude extract of the anti-dandruff and anti-itching components, and then separating and purifying the crude extract by using cation exchange resin to obtain the anti-dandruff and anti-itching components.
2. The method for extracting the components for removing dandruff and relieving itching of the traditional Chinese medicine according to claim 1, which is characterized in that: the dandruff-removing and itching-relieving component is dictamnine and/or matrine.
3. The application of sodium sulfite and 1-amino-3-methyl urea is at least one of the following 1) to 2):
1) Improving the extraction amount of the Chinese medicinal components for removing dandruff and relieving itching;
2) The extraction time of the Chinese medicinal dandruff removing and itching relieving components is reduced; the traditional Chinese medicine is cortex Dictamni and/or radix Sophorae Flavescentis, and the dandruff removing and itching relieving component is dictamnine and/or matrine.
4. A manufacturing process of shampoo comprises the following steps: the method for extracting dandruff-removing and antipruritic ingredients from Chinese herbs according to claim 1,
s1, heating water to 80-90 ℃, sequentially adding lauryl polyether ammonium sulfate, cocamidopropyl betaine, lauryl ammonium sulfate, alkyl glucoside, cocamide and a chelating agent, and uniformly stirring and mixing;
s2, cooling the water to 55-65 ℃, adding emulsified silicone oil, amino silicone oil, an anti-dandruff and itching-relieving component and a pearling agent, and stirring and mixing uniformly; the anti-dandruff and antipruritic component is extracted by the method of claim 1;
and S3, cooling the water to 40-50 ℃, sequentially adding the preservative, the reinforcing agent, the essence and the pH regulator, and uniformly stirring and mixing.
5. A process for preparing a shampoo as claimed in claim 4, which comprises the steps of: the enhancer is 2-amino-4-carbamyl butyric acid and acesulfame potassium.
6. A process for preparing a shampoo as claimed in claim 4, which comprises the steps of: the mass ratio of 2-amino-4-carbamyl butyric acid to acesulfame potassium in the enhancer is 1: mixing at a ratio of 0.3-2.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101005972B1 (en) * | 2010-02-24 | 2011-01-05 | 양재열 | Composition for exteneral application to skin and preparation method thereof |
| CN107095824A (en) * | 2017-06-21 | 2017-08-29 | 汕头市深特宝洁实业有限公司 | A kind of plant anti-dandruff shampoo and its preparation |
| CN109528588A (en) * | 2018-12-21 | 2019-03-29 | 佛山市南海施乐华化妆品有限公司 | Shampoo of anti-dandruff and itching-relieving and preparation method thereof |
| CN110327271A (en) * | 2019-08-26 | 2019-10-15 | 章华化妆品科技有限公司 | A kind of anti-dandruff and itching-relieving plant composition, preparation method and application |
| CN110934783A (en) * | 2018-09-21 | 2020-03-31 | 珠海伊斯佳科技股份有限公司 | Dandruff-removing and itching-relieving composition containing Chinese herbal medicines, silicone oil-free amino acid shampoo containing composition, and preparation method and application of silicone oil-free amino acid shampoo |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101005972B1 (en) * | 2010-02-24 | 2011-01-05 | 양재열 | Composition for exteneral application to skin and preparation method thereof |
| CN107095824A (en) * | 2017-06-21 | 2017-08-29 | 汕头市深特宝洁实业有限公司 | A kind of plant anti-dandruff shampoo and its preparation |
| CN110934783A (en) * | 2018-09-21 | 2020-03-31 | 珠海伊斯佳科技股份有限公司 | Dandruff-removing and itching-relieving composition containing Chinese herbal medicines, silicone oil-free amino acid shampoo containing composition, and preparation method and application of silicone oil-free amino acid shampoo |
| CN109528588A (en) * | 2018-12-21 | 2019-03-29 | 佛山市南海施乐华化妆品有限公司 | Shampoo of anti-dandruff and itching-relieving and preparation method thereof |
| CN110327271A (en) * | 2019-08-26 | 2019-10-15 | 章华化妆品科技有限公司 | A kind of anti-dandruff and itching-relieving plant composition, preparation method and application |
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