CN115125130A - DNA synthesizer - Google Patents
DNA synthesizer Download PDFInfo
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- CN115125130A CN115125130A CN202210819360.9A CN202210819360A CN115125130A CN 115125130 A CN115125130 A CN 115125130A CN 202210819360 A CN202210819360 A CN 202210819360A CN 115125130 A CN115125130 A CN 115125130A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The present invention relates to a DNA synthesis apparatus comprising: the liquid storage tank group comprises a plurality of liquid storage bottles; the injection pump set comprises a plurality of injection pumps, and the input ends of the injection pumps are connected with the corresponding liquid storage bottles through pipelines; the output ends of the plurality of injection pumps are communicated with the input ports through pipelines; the input end of the micro-fluidic chip is communicated with the output port of the multi-channel switching valve through a pipeline; the waste liquid bottle is communicated with the output end of the microfluidic chip through a pipeline; and the PLC is electrically connected with the injection pump set and used for controlling each injection pump and the action of the multi-channel switching valve. The DNA synthesizer optimizes the internal circuit and the control system of the equipment, utilizes the injection pump to accurately control the amount of reagent consumables, saves resources and avoids cross contamination among liquids.
Description
Technical Field
The invention belongs to the field of DNA synthesis, and particularly relates to a DNA fragment synthesis device, which is used for synthesizing DNA fragments.
Background
Generally, human has taken a step in the post-gene era, and only understanding the composition of gene sequences has failed to meet the needs of scientific development, while scientists have achieved a rather unsophisticated result in the fields of biology, medicine, etc. by means of the artificial synthesis of DNA technology, one of the methods for studying gene sequences at present is to automatically synthesize target DNA fragments by means of a DNA synthesizer, thereby conducting subsequent studies.
The DNA synthesizer is an instrument capable of performing biosynthesis in a reaction vessel, and automatically links amino acids or nucleotides in accordance with the base sequence of a target sequence. Meanwhile, as a plurality of liquids are needed for reaction to be recycled, the reaction is carried out on a synthetic column, and cross contamination among liquid paths is easy to occur. The waste of biological enzyme is caused because the use amount of the enzyme cannot be accurate. The waste liquid after reaction can damage instruments or cause environmental pollution due to untimely treatment. This can have a significant impact on the service life of the machine. The reaction has high requirements on the running and using environments of the machine, the mutual interference among electronic circuits also makes the instrument difficult to react in a complex industrial environment, and the connection wiring in the equipment is usually long and complex, so that the daily maintenance and reconstruction are difficult.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide a DNA synthesizer, which optimizes the internal circuits and control system of the device, precisely controls the amount of reagent consumables by using syringe pumps, saves resources, and avoids cross contamination between liquids because each syringe pump of the liquid pumping device has a single multi-channel switching valve channel.
In order to realize the purpose, the invention adopts the following technical scheme:
the present invention provides a DNA synthesis apparatus comprising:
the liquid storage tank group comprises a plurality of liquid storage bottles, the liquid storage bottles are used for storing DNA synthesis raw materials, and the liquid storage tank group is stored at a low temperature;
the injection pump set comprises a plurality of injection pumps, each injection pump corresponds to one liquid storage bottle, and the input ends of the injection pumps are connected with the corresponding liquid storage bottles through pipelines;
the multi-channel switching valve comprises a plurality of input ports and an output port, the output ends of a plurality of injection pumps are communicated with the input ports through pipelines, and each injection pump corresponds to the input port;
the input end of the micro-fluidic chip is communicated with the output port of the multi-channel switching valve through a pipeline, and the micro-fluidic chip is used for synthesizing DNA fragments;
the waste liquid bottle is communicated with the output end of the microfluidic chip through a pipeline and is used for recovering waste liquid generated in the DNA fragment synthesis process;
the PLC controller is connected and communicated with the injection pump set through a UART protocol and an RS485 communication line and is used for controlling the action of each injection pump, and the PLC controller is connected and communicated with the multi-channel switching valve through the RS485 communication line and is used for controlling the input ports and the output ports to be communicated one to one.
Furthermore, the DNA synthesizer also comprises a three-way valve, wherein a first end of the three-way valve is communicated with an output port of the multi-channel switching valve through a pipeline to transmit passing liquid, a second end of the three-way valve is communicated with the waste liquid bottle through a pipeline to discharge initialized waste liquid, and a third end of the three-way valve is communicated with an input end of the microfluidic chip through a pipeline to perform reaction.
Furthermore, the DNA synthesis device also comprises a heating device, the heating device is in I/O control connection with the PLC, and the PLC controls the heating device to heat the microfluidic chip.
Furthermore, the heating device also comprises a temperature measuring resistor and a temperature sensor, wherein the resistance of the temperature measuring resistor linearly rises along with the rise of temperature in a measuring range, the temperature measuring resistor transmits a measured value to the temperature sensor through a transmission line, the temperature sensor connects a power line of the temperature sensor to a third wiring terminal for series power supply, and the analog input module sends data to the PLC through a data line of the temperature sensor after filtering and rectifying for real-time detection of reaction temperature.
Furthermore, heating device includes aluminum sheet and heating pottery, the aluminum sheet with heating pottery is connected, aluminum sheet and heating pottery are located the below of micro-fluidic chip is used for supporting and heating the micropore core flow piece.
Further, DNA synthesizer still includes refrigerating plant, refrigerating plant includes radiator fan, refrigeration piece, refrigeration fan, transistor temperature control module and power, transistor temperature control module is used for setting for required refrigeration temperature, and control the refrigeration reaches required refrigeration temperature, the refrigeration piece, one side refrigeration, the another side heats, radiator fan is used for heating the cooling refrigeration of face, the refrigeration fan be used for with the refrigeration volume of refrigeration face blow to the stock solution pond group.
Furthermore, the DNA synthesizer also comprises a base, a first wiring terminal, a second wiring terminal and a third wiring terminal, a plurality of pump data communication lines of the injection pump sets are connected with the PLC through the first wiring terminal, a plurality of power lines of the injection pump sets are connected with a power supply through the second wiring terminal, one ends of the multi-channel switching valve, the three-way valve and the heating device are connected with the PLC, and the other ends of the multi-channel switching valve, the three-way valve and the heating device are connected with the power supply through the third wiring terminal.
Further, the DNA synthesizer also comprises a first direct current relay, a second direct current relay and a third direct current relay, one end of the first direct current relay is connected with the PLC, the other end of the first direct current relay is connected with the power supply, one end of the second direct current relay is connected with the PLC, the other end of the second direct current relay is connected with the lamp strip, the lamp strip is connected with the power supply, one end of the third direct current relay is connected with the heating device, and the other end of the third direct current relay is connected with the power supply.
Further, the DNA synthesizer also comprises a base, wherein the liquid storage tank group, the injection pump group, the multi-channel switching valve, the micro-fluidic chip and the waste liquid bottle are all arranged on the base, and the base is also provided with a plurality of heat dissipation holes.
Due to the adoption of the technical scheme, the invention has the following advantages: the DNA synthesizer provided by the invention has the advantages that the number of logic units is greatly reduced through wiring, electromagnetic valves and relays, the combination structure is simple, the equipment is convenient to install and maintain, the equipment optimizes an internal circuit and a control system of the equipment through a programmable logic controller, the quantity of reagent consumables is accurately controlled by using the injection pumps, resources are saved, each injection pump of the liquid pumping equipment corresponds to an independent multi-channel switching valve channel, the cross contamination among liquids is avoided, the reaction is more sufficient in a microfluidic chip, the efficiency is improved, and after the reaction is finished, the cleaning device is used for cleaning the equipment, so that the normal use of an instrument is ensured.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Like reference numerals refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a liquid circuit of a DNA synthesizer;
FIG. 2 is a circuit of the DNA synthesizer;
FIG. 3 is an assembly configuration diagram of the DNA synthesizer.
Description of the reference numerals:
the device comprises a 1-liquid storage battery group, a 2-injection pump group, a 3-multichannel switching valve, a 4-three-way valve, a 5-waste liquid bottle, a 6-microfluidic chip, a 7-temperature measuring resistor, an 8-ceramic heating sheet, a 9-PLC, a 11-temperature sensor, a 12-connecting terminal, a 13-direct current relay, a 14-refrigerating device, a 15-base, a 121-first connecting terminal, a 122-second connecting terminal, a 123-third connecting terminal, a 131-first direct current relay, a 132-second direct current relay, a 133-third direct current relay, a 141-cooling fan, a 142-refrigerating sheet, a 143-refrigerating fan, a 144-transistor temperature control module and a 145-power supply.
Detailed Description
Exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the invention are shown in the drawings, it should be understood that the invention can be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The embodiment of the invention provides a DNA synthesis device, which comprises a liquid storage tank group, an injection pump group, a multi-channel switching valve, a three-way valve, a micro-fluidic chip, a waste liquid bottle and a PLC (programmable logic controller). The liquid storage tank group comprises a plurality of liquid storage bottles, and the liquid storage bottles are used for storing DNA synthesis raw materials; the injection pump set comprises a plurality of injection pumps, each injection pump corresponds to one liquid storage bottle, and the input end of the corresponding injection pump is connected with the liquid storage bottle through a pipeline; the multi-channel switching valve comprises a plurality of input ports and an output port, the output ends of a plurality of injection pumps are communicated with the input ports through pipelines, and each injection pump corresponds to one input port; the input end of the micro-fluidic chip is communicated with the output port through a pipeline; the waste liquid bottle is communicated with the output end of the microfluidic chip through a pipeline and is used for collecting waste liquid generated in the DNA synthesis process; the PLC is connected with the injection pump set through RS485 communication and used for controlling each injection pump to act, and the PLC is connected with the multi-channel switching valve through RS485 communication and used for controlling different input ports to be communicated with the output hole. The DNA synthesizer has optimized equipment internal circuit and control system through programmable logic controller PLC, utilizes the volume of the accurate control reagent consumptive material of syringe pump, resources are saved, every syringe pump of beating liquid equipment all has solitary multichannel diverter valve passageway to correspond, has avoided the cross contamination between the liquid, and the reaction lets the reaction more abundant in the micro-fluidic chip, and efficiency obtains promoting, and after the reaction, uses belt cleaning device to carry out equipment cleaning, guarantees the normal use of instrument, extension machine life.
Example 1
The embodiment of the invention provides a DNA synthesis device, which comprises a base, and a liquid storage tank group 1, an injection pump group 2, a multi-channel switching valve 3, a three-way valve 4, a micro-fluidic chip 6, a waste liquid bottle 5 and a PLC (programmable logic controller) 6 which are arranged on the base 15. In order to facilitate heat dissipation, a plurality of heat dissipation holes are further formed in the base.
The liquid storage tank group 1 comprises 6 liquid storage bottles, wherein four liquid storage bottles are used for containing the base and the enzyme for DNA synthesis, one liquid storage bottle is used for containing a cleaning agent, and the other liquid storage bottle is used for containing a deprotection agent. The 6 liquid storage bottles are respectively communicated with the injection pump set 2 through different pipelines.
The injection pump set comprises 6 injection pumps, each injection pump corresponds to one liquid storage bottle, and the input end of each injection pump is connected with the corresponding liquid storage bottle through a pipeline. The injection pump group 2 can be accurate control and beat the volume of liquid at every turn, all can have flow and velocity of flow to show before beating at every turn to ensure the precision of reaction, avoid causing unnecessary waste, during the liquid in the liquid storage pond group 1 passes through the infusion pipeline and conveys the syringe pump, the syringe pump is again with liquid according to the order to transmission in the multichannel diverter valve 3, corresponds the liquid that the pipeline input corresponds.
The multi-channel switching valve 3 comprises six input ports and one output port, and the output ends of the six injection pumps are communicated with the corresponding input ports through pipelines; the input end of the micro-fluidic chip 6 is communicated with the output port through a pipeline, the input port and the output port are inverted cone joints, and each inverted cone joint has an anti-backflow function and can only flow out liquid when subjected to pressure.
The input end of the microfluidic chip 6 is communicated with the output port through a pipeline. The different reservoirs provide the liquids required for synthesizing DNA to the microfluidic chip 6 through the syringe pump and the switching valve. The PLC 9 is electrically connected with the injection pump set 2 and used for controlling each injection pump to act, and the PLC 9 is electrically connected with the multi-channel switching valve 3 and used for controlling different input ports to be communicated with the output port.
In the process of DNA synthesis, firstly, groups in the microfluidic chip 6 are cleaned through the cleaning solution, then different liquid storage bottles are controlled by the PLC 9 to provide required basic groups and biological enzymes, and finally, a deprotection agent is provided in the microfluidic chip 6 under the control of the PLC 9 to fix the synthesized DNA.
The DNA synthesizer has optimized the equipment internal circuit and control system through programmable logic controller 9, utilizes the volume of the accurate control reagent consumptive material of syringe pump, resources are saved, it all has 3 passageways of solitary multichannel diverter valve to correspond to beat every syringe pump of liquid equipment, has avoided the cross contamination between the liquid, and the reaction lets the reaction more abundant in micro-fluidic chip 6, and efficiency obtains promoting, and after the reaction, uses belt cleaning device to carry out equipment cleaning, guarantees the normal use of instrument.
The DNA synthesizer includes three connection terminals 12, which are a first connection terminal 121, a second connection terminal 122, and a third connection terminal 123, respectively. The first connection terminal 121 is used for data signal communication between a plurality of syringe pump sets 2 and the PLC controller 9, the second connection terminal 122 is used for connecting a plurality of syringe pump sets 2 with the power supply 10, the PLC controller 9 and the syringe pump sets 2 are connected with the power supply 10 through the third connection terminal 123, and the power supply 10 is used for supplying power to the syringe pump sets 2 and the PLC controller 9.
Each injection pump of the injection pump group 2 uses two data transmission lines, twelve data lines in total, the data transmission lines are divided into an A end and a B end, six A ends of the data transmission lines are connected in series through a first wiring terminal 121, then six B ends are connected in series, two total data lines led out from the other side of the first wiring terminal are connected into the PLC 9, and partial wiring of the data transmission of the injection pump group 2 is completed. The PLC controller 9 is an S1200PLC controller.
Each injection pump of the injection pump group 2 uses two power lines, twelve power lines in total, the power lines are divided into positive electrodes and negative electrodes, 24V DC (direct current) is needed, six power positive electrodes of the power lines are connected in series through a second connecting terminal 122, six power negative electrodes are connected in series, two pump power buses led out from the other side of the second connecting terminal 122 are connected into the 51DC24V industrial direct current power supply 10, and the wiring of the power supply part of the injection pump group 2 is completed.
The switching valve data line for controlling the operation of the multi-channel switching valve 3 is directly connected to the PLC 9 through the adapter to complete the data part wiring of the multi-function switching valve 3, and the power line of the multi-function switching valve 3 is directly supplied with power in series through the third wiring terminal 123.
In order to initialize the pipeline and discharge the air in the pipeline, the DNA synthesizer also comprises a three-way valve 4, wherein the first end of the three-way valve 4 is communicated with the output port of the multi-channel switching valve 3 through a pipeline, the second end of the three-way valve is communicated with the waste liquid bottle 5 through a pipeline, and the third end of the three-way valve is communicated with the microfluidic chip 6 through a pipeline. Before the synthetic fluid is provided for the microfluidic chip 6, air in a pipeline is emptied through the communication between the three-way valve 4 and the waste liquid bottle 5, so that the amount of reagent consumables is accurately controlled by using an injection pump, resources are saved, and smooth reaction is guaranteed. One end of the three-way valve 4 is in I/O control connection with the PLC controller 9, the PLC controller 9 controls the conduction and the closing of different channels of the three-way valve 4, and the other end of the three-way valve 4 is connected with the power supply 10 through the third connection terminal 123.
DNA synthesizer still includes heating device, heating device includes on aluminum sheet and the ceramic heating piece 8, micro-fluidic chip 6 is placed aluminum sheet and the ceramic heating piece 8 are last, ceramic heating piece 8 one end with 9 IO control connection of PLC controller, the other end passes through third binding post 123 with the power 10 electricity is connected, PLC controller 123 control the heating temperature of ceramic heating piece 8. The heating device further comprises a temperature measuring resistor 7 and a temperature sensor 11, the resistance value of the temperature measuring resistor 7 linearly rises along with the rise of temperature in a measuring range, the temperature measuring resistor 7 transmits the measured value to the temperature sensor 11 through a transmission line, the temperature sensor 11 connects a power line of the temperature sensor to a third wiring terminal 123 for series power supply, and data are sent to an analog input module of the PLC 9 through a temperature sensor data line after being rectified by filtering to complete a temperature control system.
When the device is operated, an operating light strip 16 is flashed to indicate that the system is operating, one end of the light strip is electrically connected to the PLC controller 9, the other end of the light strip is connected to a power supply through the third connection terminal 123, and the PLC controller 9 controls the operation of the light strip 16.
The DNA synthesizer further comprises three direct current relays 13 which are respectively a first direct current relay 131, a second direct current relay 132 and a third direct current relay 133, one end of the first direct current relay 131 is connected with the PLC controller 9, the other end of the first direct current relay 131 is connected with the power supply 10, one end of the second direct current relay 132 is connected with the PLC controller 9, the other end of the second direct current relay is connected with the light strip 16, the light strip 16 is connected with the power supply 10, one end of the third direct current relay 133 is connected with the heating device, and the other end of the third direct current relay 123 is connected with the power supply 10.
The DNA synthesis device also comprises a refrigerating device 14 for refrigerating the liquid storage tank group 1 to keep the refrigerating system at low temperature for a long time. The refrigerating device 14 comprises a cooling fan 141, a refrigerating sheet 142, a refrigerating fan 143, a transistor temperature control module 144 and a power source 145, the transistor temperature control module 144 is used for controlling the refrigerating temperature of the refrigerating sheet 142, one side of the refrigerating sheet 132 refrigerates, the other side of the refrigerating sheet heats, the cooling fan 141 is used for cooling and refrigerating the heating surface, and the refrigerating fan 143 is used for blowing the refrigerating capacity to the liquid storage battery pack 1. In addition, the transistor temperature control module 144 can manually adjust and control the temperature of the refrigeration system to keep the temperature constant, which is beneficial to the reaction.
It is right when DNA assembles, syringe pump package 2 and cold-stored module become to arrange from top to bottom, three-way valve 4 and multichannel diverter valve 3 multichannel become to arrange from top to bottom, the distribution is favorable to shortening the length of pipeline like this, dead volume in the pipeline can become less like this, be more favorable to fully going on and having practiced thrift the material of reaction, waste has been avoided, micro-fluidic chip 6 is in waste liquid pond top, a big fan is equipped with in the equipment housing and is cooled down for all modules in the machine, still beaten the aperture on the shell, also be favorable to the cooling of equipment, in addition, PLC controller 9 and relay etc. all are favorable to the winding displacement wiring at the rear side of syringe pump, avoid unnecessary equipment to arrange, lead to the circuit confusion and so on among the equipment
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (9)
1. A DNA synthesizing apparatus comprising:
the liquid storage tank group comprises a plurality of liquid storage bottles, the liquid storage bottles are used for storing DNA synthesis raw materials, and the liquid storage tank group is stored at a low temperature;
the injection pump set comprises a plurality of injection pumps, each injection pump corresponds to one liquid storage bottle, and the input ends of the injection pumps are connected with the corresponding liquid storage bottles through pipelines;
the multi-channel switching valve comprises a plurality of input ports and an output port, the output ends of a plurality of injection pumps are communicated with the input ports through pipelines, and each injection pump corresponds to the input port;
the input end of the micro-fluidic chip is communicated with the output port of the multi-channel switching valve through a pipeline, and the micro-fluidic chip is used for synthesizing DNA fragments;
the waste liquid bottle is communicated with the output end of the microfluidic chip through a pipeline and is used for recovering waste liquid generated in the DNA fragment synthesis process;
the PLC controller is connected and communicated with the injection pump set through communication lines and used for controlling the action of each injection pump, and the PLC controller is connected and communicated with the multi-channel switching valve through communication lines and used for controlling the different input ports to be communicated with the output ports in a one-to-one mode.
2. The DNA synthesizer of claim 1, further comprising a three-way valve, wherein a first end of the three-way valve is connected to the output port of the multi-channel switching valve via a pipeline for transferring the liquid therethrough, a second end of the three-way valve is connected to the waste liquid bottle via a pipeline for discharging the initialized waste liquid, and a third end of the three-way valve is connected to the input port of the microfluidic chip via a pipeline for reaction.
3. The DNA synthesis device of claim 2, further comprising a heating device, wherein the heating device is in I/O control connection with the PLC controller, and the PLC controller controls the heating device to heat the microfluidic chip.
4. The DNA synthesizer according to claim 3, wherein the heater further comprises a temperature measuring resistor and a temperature sensor, the resistance of the temperature measuring resistor increases linearly with the increase of temperature in the measurement range, the temperature measuring resistor transmits the measured value to the temperature sensor through a transmission line, the temperature sensor connects a power line of the temperature sensor to a third connection terminal for series power supply, and the analog input module which sends data to the PLC through a data line of the temperature sensor after the data is rectified by filtering detects the reaction temperature in real time.
5. The DNA synthesizing apparatus according to claim 4, wherein the heating means comprises an aluminum plate and a heating ceramic, the aluminum plate being connected to the heating ceramic, the aluminum plate and the heating ceramic being located below the microfluidic chip for supporting and heating the microfluidic chip.
6. The DNA synthesis device according to claim 1, further comprising a refrigerating device, wherein the refrigerating device comprises a cooling fan, a refrigerating sheet, a refrigerating fan, a transistor temperature control module and a power supply, the transistor temperature control module is used for setting a required refrigerating temperature and controlling the refrigerating to reach the required refrigerating temperature, one surface of the refrigerating sheet is used for refrigerating, the other surface of the refrigerating sheet is used for heating, the cooling fan is used for cooling and refrigerating the heating surface, and the refrigerating fan is used for blowing the refrigerating capacity of the heating surface to the liquid storage tank group.
7. The DNA synthesis apparatus according to claim 5, further comprising a first terminal, a second terminal, and a third terminal, wherein the pump data communication lines of the plurality of syringe pump sets are connected to the PLC controller via the first terminal, the power lines of the plurality of syringe pump sets are connected to a power supply via the second terminal, and one ends of the multi-channel switching valve, the three-way valve, and the heating device are connected to the PLC controller, and the other ends thereof are connected to the power supply via the third terminal.
8. The DNA synthesizing apparatus according to claim 3, further comprising a first DC relay, a second DC relay and a third DC relay, wherein one end of the first DC relay is connected to the PLC controller and the other end thereof is connected to a power supply, one end of the second DC relay is connected to the PLC controller and the other end thereof is connected to a lamp strip and is connected to the power supply through the lamp strip, and one end of the third DC relay is connected to the heating apparatus and the other end thereof is connected to the power supply through the third DC relay.
9. The DNA synthesizer according to claim 1, further comprising a base, wherein the reservoir, the syringe pump set, the multi-channel switching valve, the microfluidic chip and the waste liquid bottle are all mounted on the base, and the base is further provided with a plurality of heat dissipation holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210819360.9A CN115125130A (en) | 2022-07-13 | 2022-07-13 | DNA synthesizer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210819360.9A CN115125130A (en) | 2022-07-13 | 2022-07-13 | DNA synthesizer |
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| CN115125130A true CN115125130A (en) | 2022-09-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109825431A (en) * | 2019-03-18 | 2019-05-31 | 中国人民解放军军事科学院军事医学研究院 | Oligonucleotides assemble in situ instrument |
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2022
- 2022-07-13 CN CN202210819360.9A patent/CN115125130A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109825431A (en) * | 2019-03-18 | 2019-05-31 | 中国人民解放军军事科学院军事医学研究院 | Oligonucleotides assemble in situ instrument |
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