CN115125101A - Microbial fermentation detection device - Google Patents

Microbial fermentation detection device Download PDF

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Publication number
CN115125101A
CN115125101A CN202210902564.9A CN202210902564A CN115125101A CN 115125101 A CN115125101 A CN 115125101A CN 202210902564 A CN202210902564 A CN 202210902564A CN 115125101 A CN115125101 A CN 115125101A
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China
Prior art keywords
culture
cavity
culture dish
partition plate
transparent cover
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Pending
Application number
CN202210902564.9A
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Chinese (zh)
Inventor
尚广红
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Guangzhou Kunsheng Medical Products Co ltd
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Guangzhou Kunsheng Medical Products Co ltd
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Priority to CN202210902564.9A priority Critical patent/CN115125101A/en
Publication of CN115125101A publication Critical patent/CN115125101A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a microbial fermentation detection device, which comprises a culture dish and a transparent cover arranged on the culture dish, wherein the culture dish comprises a circular culture cavity and a partition plate which is positioned in the culture cavity and used for forming a separation cavity at the bottom of the culture cavity, a bulge positioned in the culture cavity is arranged at the circle center of the partition plate, a plurality of pores are arranged on the bulge, when fungus beads are injected into the culture dish in such a way, the fungus beads cannot contact with air, and pollution can be prevented and avoided, a treatment rod is arranged in the culture dish and can be uniformly smeared by rotating the treatment rod in the culture dish, the treatment rod rotates stably, the fungus beads are uniformly smeared in the culture dish, the heating time can be reduced when the culture rod is placed in a thermal insulation device for culture, so that the working efficiency is improved, and after the culture is finished, the fungus can be in a relatively closed state and cannot contact with external bacteria when the culture rod is detected, the accuracy of the data obtained during detection is ensured.

Description

Microbial fermentation detection device
Technical Field
The invention relates to the technical field of microbial fermentation detection, in particular to a microbial fermentation detection device.
Background
The microbial fermentation detection device is composed of a detector and a culture dish for placing a microbial fermentation sample on the detector, wherein the culture dish detection device is provided with a transparent cover, the microbial fermentation sample is placed in heat preservation equipment for heat preservation treatment through the culture dish when the microbial fermentation detection device is used, the culture dish is taken out of the heat preservation equipment after waiting for several days and then is placed on the microbial fermentation detector for detection, the transparent cover needs to be detached from the culture dish in the detection process, and then a detection probe of the detector is extended into the culture dish for strain detection.
It can be seen from the whole detection process that whether the microbial strains are placed in the culture dish or are taken out of the culture dish after the microbial strains are cultured, the transparent cover on the culture dish needs to be removed, and the culture dish is exposed in the air, namely, the microbial strains are exposed in the air during the placing process and the detection process of the cultured microbial strains during the culture process, so that the microbial strains contact with bacteria in the air, and the accuracy of the obtained detection data is influenced.
Disclosure of Invention
Based on the above problems, the present invention provides a microbial fermentation detection apparatus.
In order to achieve the purpose, the invention provides the following technical scheme:
a microbial fermentation detection device comprises a culture dish and a transparent cover arranged on the culture dish, wherein the culture dish comprises a circular culture cavity and a partition plate which is positioned in the culture cavity and used for forming a separation cavity at the bottom of the culture cavity, a bulge positioned in the culture cavity is arranged at the circle center of the partition plate, a plurality of pores are formed in the bulge, one end of each pore enters the culture cavity along the bulge and is communicated with the culture cavity, the other end of each pore is communicated with the bottom surface of the partition plate, a buckling seat is arranged on the bottom surface of the partition plate and faces the bottom surface of the partition plate, a rubber retaining ring is arranged in each cavity, one end of each rubber retaining ring is in contact with the bottom surface of the partition plate and covers the pore, the other end of each rubber retaining ring extends along the height direction of the corresponding cavity and then is connected to the buckling seat, and a through hole communicated with the bottom surface of the partition plate is upwards formed in the bottom of the buckling seat, the hole face of through-hole is anomalous camber face, detain the seat be with rubber fender ring equal rubber material's rubber seat, the pore is a plurality of departments, and the pore one end of a plurality of departments leads directly in bellied high point within range, the middle part of transparent cover is equipped with the guide pin bushing, the guide pin bushing has been seted up the guide hole, the top of guide hole is equipped with the bearing, installs the processing pole that can carry out the rotation action in cultivateing the intracavity through the bearing in the guide hole, it is equipped with the flexion of contact on protruding upper surface and the extension portion that extends along the radius direction of cultivateing the chamber from the flexion to handle the pole, the contact of extension portion is on the inner chamber bottom surface of cultivateing the chamber.
As further preferred: the culture dish is provided with an external thread, and the transparent cover is provided with an internal thread matched with the external thread.
As further preferred: the loading surfaces of the bending part and the extending part of the treatment rod are both triangular, and the bottoms of the bending part and the extending part are formed with planes which are contacted with the upper surface of the bulge and the bottom surface of the inner cavity of the culture cavity.
As further preferred: the processing rod is provided with a channel which is close to the upper part of the plane from one end of the bending part to one end of the extending part, and the channel is communicated with the pore at one end of the bending part.
As further preferred: the guide sleeve extends upwards from the top surface of the transparent cover, and one end of the processing rod, which is positioned in the guide sleeve, extends upwards to exceed the top of the guide sleeve and is provided with a handle corresponding to the upper part of the guide sleeve.
As further preferred: the transparent cover is penetrated with a rubber plug, a sampling gap communicated with the transparent cover and the inner cavity of the culture cavity is downwards formed in the top surface of the rubber plug, and the sampling gap is closed under the elastic action of the rubber plug.
As further preferred: handle and seted up a plurality of on the side of the extension portion of pole and grab the groove, a plurality of grab the groove along the length direction evenly distributed of extension portion to it is close to in the plane to grab the groove, handle the pole when rotatory in the cultivation intracavity, grab the groove via in the below of rubber buffer.
Compared with the prior art, the invention has the following beneficial effects:
the culture dish for detecting the microorganisms is functionally arranged, a plurality of pores are arranged in an inner cavity of the culture dish from the bottom, a buckling seat with a clamping function is arranged at the bottom of the culture dish, the bacteria beads are inserted into the buckling seat through a test tube and are fixed during biological culture, then the bacteria beads are poured into a culture cavity through the pores, a rubber retaining ring is arranged in the buckling seat, the rubber retaining ring covers the pores by utilizing the rebounding action of the rubber retaining ring after the bacteria beads flow into the culture dish, so that the bacteria beads can be prevented from flowing out of the culture dish, when the bacteria beads are injected into the culture dish in the mode, the bacteria beads cannot contact with air, the pollution can be prevented, a processing rod is arranged in the culture dish, the processing rod can uniformly smear the bacteria beads through rotation in the culture dish, the processing rod can stably rotate, the bacteria beads are uniformly smeared in the culture dish, the heating time can be reduced when the bacteria beads are placed in a thermal insulation device for culture, and the working efficiency can be improved, after the culture is finished, the strain can not contact with external bacteria under a relatively closed state during detection, so that the accuracy of data obtained during detection is ensured.
Drawings
FIG. 1 is a schematic view of the cut-away internal structure of the present invention;
FIG. 2 is a bottom schematic bottom view of the present invention as taken from FIG. 1;
FIG. 3 is an enlarged view of the portion A of FIG. 2;
FIG. 4 is a schematic view of the present invention in a cut-away configuration after disassembly;
FIG. 5 is a schematic view of the present invention with the transparent cover removed;
FIG. 6 is a schematic view of the cleaning rod alone according to the present invention;
fig. 7 is a schematic view of the internal structure of the rubber stopper of the present invention in a front view plane with the rubber stopper cut open, wherein B denotes a detection rod of a detection instrument;
FIG. 8 is a schematic view of the position relationship and structure of the fine hole, the fastening seat, the through hole and the rubber stopper ring of the present invention.
Description of the main reference numbers:
1. a culture dish; 2. a transparent cover; 3. a culture chamber; 4. a separation chamber; 5. a partition plate; 6. a protrusion; 7. fine pores; 8. a buckle seat; 81. a through hole; 9. a lumen; 10. a rubber retainer ring; 11. a guide sleeve; 12. a guide hole; 13. a bearing; 14. a treatment rod; 15. a bending section; 16. an extension part; 17. a plane; 18. a channel; 19. a handle; 20. a rubber plug; 21. sampling a gap; 22. grabbing a groove; 23. a slot cavity; 24. a power supply component; 25. covering; 26. a button; 27. a germicidal lamp.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (8)

1. The utility model provides a microbial fermentation detection device, includes culture dish (1) and sets up transparent lid (2) on culture dish (1), its characterized in that: the culture dish (1) comprises a circular culture cavity (3) and a partition plate (5) which is positioned in the culture cavity (3) and used for enabling the bottom of the culture cavity (3) to be formed with a separation cavity (4), a bulge (6) which is positioned in the culture cavity (3) is arranged at the circle center of the partition plate (5), a plurality of pores (7) are formed in the bulge (6), one ends of the pores (7) enter into the culture cavity (3) along the bulge (6) and are communicated with the culture cavity, the other ends of the pores are communicated with the bottom surface of the partition plate (5), a buckling seat (8) is arranged on the bottom surface of the partition plate (5), a cavity channel (9) is arranged on the bottom surface of the buckling seat (8) facing the partition plate (5), a rubber baffle ring (10) is arranged in the cavity channel (9), one end of the rubber baffle ring (10) is contacted on the bottom surface of the partition plate (5) and covers the pores (7), and the other end of the rubber baffle ring (10) is connected to the buckling seat (8) after extending along the height direction of the cavity channel (9), a through hole (81) communicated with the bottom surface of the partition plate (5) is upwards formed at the bottom of the buckling seat (8), the hole surface of the through hole is an irregular curvature surface, the buckling seat (8) is a rubber seat made of rubber materials the same as the rubber baffle ring (10), the fine holes (7) are arranged at a plurality of positions, one ends of the fine holes (7) at the plurality of positions are directly communicated with the high point range of the bulge (6), a guide sleeve (11) is arranged at the middle part of the transparent cover (2), the guide sleeve (11) is provided with a guide hole (12), a bearing (13) is arranged at the top end of the guide hole (12), a processing rod (14) which can rotate in the culture cavity (3) is arranged in the guide hole (12) through the bearing (13), the processing rod (14) is provided with a bending part (15) contacted with the upper surface of the bulge (6) and an extending part (16) extending from the bending part (15) along the radius direction of the culture cavity (3), the extension part (16) is contacted on the inner cavity bottom surface of the culture cavity (3).
2. The microbial fermentation detection apparatus according to claim 1, wherein: the culture dish (1) is provided with an external thread, and the transparent cover (2) is provided with an internal thread matched with the external thread.
3. The microbial fermentation detection apparatus according to claim 1, wherein: the loading surfaces of the bending part (15) and the extending part (16) of the processing rod (14) are both triangular, and the bottoms of the bending part (15) and the extending part (16) are provided with planes (17) which are contacted with the upper surface of the bulge (6) and the bottom surface of the inner cavity of the culture cavity (3).
4. The microbial fermentation detection apparatus according to claim 3, wherein: the processing rod (14) is provided with a channel (18) which is close to the upper part of the plane (17) from one end of the bending part (15) to one end of the extending part (16), and the channel (18) is communicated with the pore (7) at one end of the bending part (15) up and down.
5. The apparatus for detecting fermentation of microorganisms according to claim 4, wherein: the guide sleeve (11) extends upwards from the top surface of the transparent cover (2), one end of the processing rod (14) positioned in the guide sleeve (11) extends upwards to exceed the top of the guide sleeve (11) and is provided with a handle (19) corresponding to the upper part of the guide sleeve (11).
6. The apparatus for detecting fermentation of microorganisms according to claim 5, wherein: the utility model discloses a culture medium, including rubber buffer (3), transparent cover (2) go up to run through on transparent cover (2) have run through rubber buffer (20), from the top surface of rubber buffer (20) has been seted up downwards with transparent cover (2) and culture chamber (3) inner chamber communicating sampling gap (21), sampling gap (21) are in the closure under rubber buffer (20) elastic action.
7. The apparatus for detecting fermentation of microorganisms according to claim 6, wherein: handle and seted up a plurality of on the side of the extension portion (16) of pole (14) and grab groove (22), a plurality of grab groove (22) along the length direction evenly distributed of extension portion (16) to grab groove (22) and be close to in plane (17), handle pole (14) when cultivateing chamber (3) internal rotation, grab groove (22) and pass through in the below of rubber buffer (20).
8. The apparatus for detecting fermentation of microorganisms according to claim 7, wherein: handle (19) extend and seted up vallecular cavity (23) towards the radial direction of transparent cover (2), install power supply module (24) in vallecular cavity (23), buckle closure (25) are installed at the top of handle (19), are equipped with button (26) of electric connection in power supply module (24) on buckle closure (25), install bactericidal lamp (27) along flexion (15) and the profile of extension portion (16) on handling pole (14), the electric property line of bactericidal lamp (27) runs through and extends to vallecular cavity (23) in and be connected in power supply module (24) through handling pole (14).
CN202210902564.9A 2022-07-29 2022-07-29 Microbial fermentation detection device Pending CN115125101A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210902564.9A CN115125101A (en) 2022-07-29 2022-07-29 Microbial fermentation detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210902564.9A CN115125101A (en) 2022-07-29 2022-07-29 Microbial fermentation detection device

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CN115125101A true CN115125101A (en) 2022-09-30

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040029266A1 (en) * 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device
WO2010044613A2 (en) * 2008-10-15 2010-04-22 (주)나래바이오테크 Microorganism culture vessel that can be opened or closed selectively and method for preparing novel microorganism culture medium containing moisturizing additive
US20140146642A1 (en) * 2007-01-12 2014-05-29 Lbt Innovations Limited Method and apparatus for locating the surface of solid growth culture media in a plate
CN212293556U (en) * 2020-04-02 2021-01-05 重庆国际旅行卫生保健中心(重庆海关口岸门诊部) Microorganism separation culture apparatus
CN112608847A (en) * 2021-01-27 2021-04-06 张海甄 Sealed cultivation device for biological cell cultivation and use method thereof
CN213012844U (en) * 2020-07-08 2021-04-20 山东科技大学 Rotary disc capable of directly coating and inoculating bacteria
CN113337401A (en) * 2020-06-23 2021-09-03 成都艾伟孚生物科技有限公司 Culture dish and application thereof
CN115353959A (en) * 2022-08-08 2022-11-18 广州堃盛医疗用品有限公司 Microorganism detection device

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040029266A1 (en) * 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device
US20140146642A1 (en) * 2007-01-12 2014-05-29 Lbt Innovations Limited Method and apparatus for locating the surface of solid growth culture media in a plate
WO2010044613A2 (en) * 2008-10-15 2010-04-22 (주)나래바이오테크 Microorganism culture vessel that can be opened or closed selectively and method for preparing novel microorganism culture medium containing moisturizing additive
CN212293556U (en) * 2020-04-02 2021-01-05 重庆国际旅行卫生保健中心(重庆海关口岸门诊部) Microorganism separation culture apparatus
CN113337401A (en) * 2020-06-23 2021-09-03 成都艾伟孚生物科技有限公司 Culture dish and application thereof
CN213012844U (en) * 2020-07-08 2021-04-20 山东科技大学 Rotary disc capable of directly coating and inoculating bacteria
CN112608847A (en) * 2021-01-27 2021-04-06 张海甄 Sealed cultivation device for biological cell cultivation and use method thereof
CN115353959A (en) * 2022-08-08 2022-11-18 广州堃盛医疗用品有限公司 Microorganism detection device

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