CN115119779A - Method for separating and culturing single hydranth of hydranth soft coral - Google Patents
Method for separating and culturing single hydranth of hydranth soft coral Download PDFInfo
- Publication number
- CN115119779A CN115119779A CN202210993749.5A CN202210993749A CN115119779A CN 115119779 A CN115119779 A CN 115119779A CN 202210993749 A CN202210993749 A CN 202210993749A CN 115119779 A CN115119779 A CN 115119779A
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- hydranth
- coral
- separating
- sintered ceramic
- culturing
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- 241000124001 Alcyonacea Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000012258 culturing Methods 0.000 title claims abstract description 11
- 235000014653 Carica parviflora Nutrition 0.000 claims abstract description 26
- 239000000919 ceramic Substances 0.000 claims abstract description 26
- 241000243321 Cnidaria Species 0.000 claims abstract description 25
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims abstract description 20
- 229930182566 Gentamicin Natural products 0.000 claims abstract description 20
- 229960002518 gentamicin Drugs 0.000 claims abstract description 14
- 238000002791 soaking Methods 0.000 claims abstract description 11
- 235000013372 meat Nutrition 0.000 claims abstract description 3
- 239000013535 sea water Substances 0.000 claims abstract description 3
- 241000243251 Hydra Species 0.000 claims 4
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 claims 4
- 244000267823 Hydrangea macrophylla Species 0.000 claims 1
- 235000014486 Hydrangea macrophylla Nutrition 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 208000027418 Wounds and injury Diseases 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 208000014674 injury Diseases 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 7
- 241000243320 Hydrozoa Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005245 sintering Methods 0.000 description 3
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000242757 Anthozoa Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 239000004100 Oxytetracycline Substances 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000012984 antibiotic solution Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229960000625 oxytetracycline Drugs 0.000 description 1
- IWVCMVBTMGNXQD-PXOLEDIWSA-N oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 description 1
- 235000019366 oxytetracycline Nutrition 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for separating and culturing a single hydranth of hydranth soft coral. Separating single hydranth from group soft coral, and separating common meat and bottom tissue together; soaking a single hydranth coral tissue in a gentamycin solution; fixing the sintered ceramic plate in the tube to obtain a coral attaching device; and (3) placing the single hydranth soaked in the gentamicin solution on the sintered ceramic sheet to be attached to the sintered ceramic sheet, and then placing the coral attaching device in the sea water for cultivation. The single hydranth separation method is adopted, so that the injury degree of the single hydranth separated from the large hydranth soft coral is smaller; the effect of soaking in gentamicin with the concentration of 25-45 ppm enables the survival rate of later culture of a single hydranth soft coral to be highest; by adopting the attaching device disclosed by the invention, the single hydranth of the hydranth soft coral is less stimulated, the recovery time is shorter, the cultivation survival rate is higher, and the attaching probability is higher.
Description
Technical Field
The invention belongs to the technical field of artificial cultivation of coral reef ecosystems, and particularly relates to a method for separating and cultivating a single hydranth of large hydranth soft coral.
Background
Soft coral in the coral reef ecosystem is a potential emerging medicine raw material and is an important marine organism resource. The soft coral is a main member of coral family, and provides considerable active compounds for human beings, such as efficient anticancer and antibacterial chemicals. However, the coral reef ecosystem is sensitive to external environmental changes, and under the dual influence of global changes and human activities, the coral reef ecosystem is rapidly degraded in recent decades, and may become the first ecosystem lost due to global climate changes. Therefore, artificial coral culture becomes an important means for preserving coral germplasm resources. Although the research techniques of single-cup separation and artificial cultivation of hermatypic corals are more, the single hydranth cultivation techniques of soft corals are less. The culture of the hydranth soft coral can maximally reduce optical competition among soft coral groups and improve the growth rate. In addition, the response of hydranth soft coral to global climate change is also receiving more and more attention. In order to quantitatively observe and research the soft coral better, the culture of the hydranth soft coral is critical, so that the enhancement of the artificial culture of the hydranth soft coral is very necessary.
Disclosure of Invention
The invention aims to provide a method for separating and culturing a single hydranth of large hydranth soft coral, which has shorter recovery time and higher survival rate.
The single hydranth separation and culture method of the large hydranth soft coral comprises the following steps of:
separating single hydranth from group soft coral, and separating common meat and bottom tissue together; soaking a single hydranth coral tissue in a gentamycin solution;
fixing the sintered ceramic plate in the tube to obtain a coral attaching device;
placing a single hydranth soaked in a gentamicin solution on a sintered ceramic sheet to be attached to the sintered ceramic sheet, and then placing a coral attaching device in seawater for cultivation.
Preferably, the gentamicin solution is 25-45 ppm gentamicin solution.
Preferably, the step of soaking in the gentamicin solution is to soak in the gentamicin solution with the concentration of 25ppm for 10 min.
Preferably, the tube is a PVC tube.
Preferably, a gap is formed between the sintered ceramic plate and the pipe, or the sintered ceramic plate is provided with a hole so that water can flow in the pipe.
Preferably, the sintered ceramic sheet is a regular hexagonal sintered ceramic sheet.
Preferably, the ceramic sintering piece and the PVC pipe have the same diameter, so that the ceramic sintering piece is conveniently clamped and fixed in the PVC pipe.
Compared with the prior art, the invention has the following beneficial effects:
the single hydranth separation method is adopted, so that the injury degree of the single hydranth separated from the large hydranth soft coral is smaller; compared with wide soaking by using an antibiotic solution, the effect of soaking by using gentamicin with the concentration of 25-45 ppm enables the survival rate of the single hydranth soft coral in later culture to be highest; meanwhile, compared with the traditional method for fixing by using a rubber band, a fish line or an alexandric, the attachment device provided by the invention has the advantages that the irritation to a single hydranth of a soft coral is smaller, the recovery time is shorter, the cultivation survival rate is higher, the attachment probability is higher, and the attachment mode is a soft coral early-stage attachment mode without binding and gluing. Meanwhile, the method has the advantages that the adopted materials are low in price, the manufacturing is simple, the mass production of the artificial culture of the large hydroid soft coral can be effectively realized, the diversity of the submarine coral can be increased after the artificial culture, and meanwhile, sufficient experimental samples can be provided for the research in the field of biological medicines and the research on the influence of environmental stress on the soft coral. .
Drawings
FIG. 1 is a schematic view of the coral attachment apparatus, in which 1, a PVC pipe; 2. a single hydranth; 3. and sintering the ceramic wafer.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1:
1. first, a single hydranth with sufficient marginal coprocessing tissue and basal tissue was separated from the parent large hydranth soft coral using a scalpel.
2. Soaking a single hydranth coral tissue in a solution for inhibiting the growth of microorganisms for 10min (table 1), wherein common antibiotics comprise gentamycin, oxytetracycline, tetracycline, penicillin, furan drugs and the like, and soaking in 45ppm gentamycin for 10min can lead the death rate of the single hydranth in the later artificial cultivation process to be the lowest, but in combination with the concentration and the survival rate, soaking in 25ppm gentamycin for 10min is selected as the best choice. The gentamicin obviously reduces the death rate of a single hydroid and effectively prevents the bacterial infection of a section of the single hydroid.
TABLE 1
3. Manufacturing a coral attachment device, wherein the structure of the coral attachment device is shown in figure 1, the coral attachment device consists of a sintered ceramic plate 3 and a PVC pipe 1 with the same diameter as the ceramic plate, and the coral attachment device is obtained by sleeving the PVC pipe on the sintered ceramic plate;
4. and (3) placing the soaked hydranth 2 on a sintered ceramic sheet of a coral attaching device, attaching the hydranth to the sintered ceramic sheet, and placing the coral attaching device in a sea vat for cultivation. After three days, the adhesion is stable, and the adhesion rate is 100%. The attachment rate of the soft coral of a single hydranth cultured by the method is 100%, and the final survival rate can reach more than 95%.
Claims (7)
1. A method for separating and culturing a single hydranth of Hydra hydrangea, which is characterized by comprising the following steps:
separating single hydranth from group soft coral, and separating common meat and bottom tissue together; soaking a single hydranth coral tissue in a gentamycin solution;
fixing the sintered ceramic plate in the tube to obtain a coral attaching device;
placing a single hydranth soaked in a gentamicin solution on a sintered ceramic sheet to be attached to the sintered ceramic sheet, and then placing a coral attaching device in seawater for cultivation.
2. The method for separating and culturing a single hydranth of hydranth soft coral according to claim 1, wherein the gentamicin solution is 25-45 ppm gentamicin solution.
3. The method for separating and culturing individual hydranth of hydranth soft coral according to claim 1, wherein the soaking in gentamicin solution is carried out for 10min at a concentration of 25ppm gentamicin solution.
4. The method for isolating and culturing a single hydranth of Hydra according to claim 1, wherein the tube is a PVC tube.
5. The method for separating and culturing the individual hydranth of claim 1, wherein a gap exists between the sintered ceramic sheet and the tube, or the sintered ceramic sheet is provided with holes.
6. The method for separating and culturing a single hydranth of Hydra according to claim 1 or 5, wherein the sintered ceramic wafer is a regular hexagonal sintered ceramic wafer.
7. The method for separating and culturing a single hydranth of Hydra according to claim 4, wherein the ceramic sintered plate and the PVC pipe have the same diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210993749.5A CN115119779A (en) | 2022-08-18 | 2022-08-18 | Method for separating and culturing single hydranth of hydranth soft coral |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210993749.5A CN115119779A (en) | 2022-08-18 | 2022-08-18 | Method for separating and culturing single hydranth of hydranth soft coral |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115119779A true CN115119779A (en) | 2022-09-30 |
Family
ID=83387542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210993749.5A Pending CN115119779A (en) | 2022-08-18 | 2022-08-18 | Method for separating and culturing single hydranth of hydranth soft coral |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115119779A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008125440A (en) * | 2006-11-21 | 2008-06-05 | Npo Environmental Technology Center | Method for culturing coral |
| CN201383997Y (en) * | 2009-01-09 | 2010-01-20 | 吕军仪 | Factory semi-dry/wet three-dimensional lower animal breeding device |
| US20110179504A1 (en) * | 2007-09-17 | 2011-07-21 | Ramot at Tel-Aviv Univeristy Ltd. | Method For Coral Tissue Cultivation And Propagation |
| CN103146638A (en) * | 2013-03-14 | 2013-06-12 | 青岛农业大学 | Induced proliferation method for glehnia littoralis hairy roots |
| CN103583471A (en) * | 2013-11-11 | 2014-02-19 | 海南省水产研究所 | Method for artificial rapid propagation expanding of succulent soft coral |
| TWI746273B (en) * | 2020-11-19 | 2021-11-11 | 國立臺灣海洋大學 | Coral culture substrate with embedded base and attached depression |
-
2022
- 2022-08-18 CN CN202210993749.5A patent/CN115119779A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008125440A (en) * | 2006-11-21 | 2008-06-05 | Npo Environmental Technology Center | Method for culturing coral |
| US20110179504A1 (en) * | 2007-09-17 | 2011-07-21 | Ramot at Tel-Aviv Univeristy Ltd. | Method For Coral Tissue Cultivation And Propagation |
| CN201383997Y (en) * | 2009-01-09 | 2010-01-20 | 吕军仪 | Factory semi-dry/wet three-dimensional lower animal breeding device |
| CN103146638A (en) * | 2013-03-14 | 2013-06-12 | 青岛农业大学 | Induced proliferation method for glehnia littoralis hairy roots |
| CN103583471A (en) * | 2013-11-11 | 2014-02-19 | 海南省水产研究所 | Method for artificial rapid propagation expanding of succulent soft coral |
| TWI746273B (en) * | 2020-11-19 | 2021-11-11 | 國立臺灣海洋大學 | Coral culture substrate with embedded base and attached depression |
Non-Patent Citations (1)
| Title |
|---|
| 陈彬等: "《海洋生态恢复理论与实践》", 31 January 2012, 海洋出版社, pages: 69 * |
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