CN115104675A - Low-copper-zinc environment-friendly biological feed - Google Patents
Low-copper-zinc environment-friendly biological feed Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to the technical field of biology, and discloses a low-copper-zinc environment-friendly biological feed, which provides the following components for each kilogram of basic feed: cu 40-50 mg; 45-55mg of Zn; proper amount of compound vitamin; a proper amount of complex enzyme; a micro-ecological agent; the Cu is in the form of copper amino acid, and the Zn is in the form of zinc amino acid. The organic copper and zinc are comprehensively applied to scientific compatibility with biological enzyme, microecologics and the like, so that comprehensive balance among various trace elements and between the trace elements and other nutrients is realized, the addition amount of copper and zinc can be effectively reduced from a feed source, the excretion amount of heavy metals such as copper and zinc in excrement is reduced, the intestinal microecological balance of piglets can be improved, the production performance is improved, and the economic benefit and the ecological environmental benefit of pig breeding are well considered.
Description
Technical Field
The invention relates to the field of biotechnology, in particular to a low-copper-zinc environment-friendly biological feed.
Background
Copper and zinc are trace elements necessary for the growth and development of animals. The deficiency of trace elements can cause slow growth and reduced disease resistance of animals. NRC "nutritional needs of pigs" (2012) in the united states recommends that the copper and zinc requirements of pigs are less than 6mg/kg and 100mg/kg, respectively. A large number of researches show that the high-copper and high-zinc pig feed has the effects of resisting diarrhea and promoting growth of piglets. Because the copper sulfate and the zinc sulfate used for the feed are cheap, and the addition amount of the copper sulfate and the zinc sulfate in the feed is very low, the copper sulfate and the zinc sulfate are often added in the feed by tens of times of the required amount in pig production for decades so as to obtain the optimal production performance. Along with the increase of the copper and zinc level of the feed, the utilization rate of the pig on the copper and zinc is reduced, and the emission of the copper and zinc in the manure is increased. After the growing pigs are fed with the high-copper (250mg/kg) daily ration, the content of the copper in the manure is 7.6 times that of the low-copper (10mg/kg) daily ration.
The breeding pollution is a bottleneck which restricts the rapid development of the modern pig industry for a long time. Ecological environment protection and ecological civilization construction are the basic national policy of China. The development of environment-friendly breeding industry is a general plan which accords with the national sustainable development of modern animal husbandry. Different from the biological degradation of organic pollutants such as carbohydrates, proteins, nitrogen and phosphorus in excrement, the copper and zinc which cannot be digested and absorbed by animals have the characteristics of low content (mg/kg level), great harm, incapability of degradation and easy accumulation in soil and crops along with excrement excretion, so that the quality of soil and ecological environment around a farm are seriously threatened due to the overhigh content of heavy metals such as copper and zinc in the excrement. In addition, the high copper and zinc in the feed also interfere with the intestinal microecological balance and affect the health of animals.
The 2625 bulletin of the agricultural rural sector requires that the maximum copper content in the feed for piglets (< 25kg) is reduced from 250mg/kg (1224 bulletin of the agricultural rural sector in 2009) to 125mg/kg and the maximum zinc content in the feed is reduced from 250mg/kg to 110 mg/kg. In the future, the highest limit of copper and zinc in the feed is further reduced by rural parts of agriculture, which brings more serious challenges to pig breeding enterprises.
In the breeding production, inorganic copper sulfate and zinc sulfate are widely used in the preparation of pig feed as feed copper and zinc sources due to low price. Researches show that organic copper and organic zinc chelated with amino acid, small peptide and the like have higher absorption and utilization efficiency than traditional inorganic copper and inorganic zinc sources, but the organic copper and the organic zinc cannot effectively prevent piglet diarrhea in the piglet growth stage, so that the piglet production performance is difficult to improve.
Wang et al (2016) also showed that a reduction of the copper content of the feed from 300mg/kg to 150mg/kg did not affect the performance of the piglets 40 days after weaning, but that the weight gain and the feed conversion efficiency of piglets with a copper content below 100mg/kg were reduced. In addition, researches show that the basic copper chloride has higher biological value than copper sulfate, can increase the content of liver copper and ceruloplasmin of piglets and reduce the oxidative stress level of duodenum, but the basic copper chloride cannot improve the production performance of the piglets and reduce the copper addition level of a feed. The study of Zheng et al (2018) shows that the adoption of basic copper chloride (130mg/kg) instead of copper sulfate increases the level of ceruloplasmin and superoxide dismutase, but tends to reduce the production performance of fattening pigs. Therefore, it is difficult to simply reduce the addition of copper and zinc in the feed to ensure that the production performance of animals is not reduced.
Perez et al (2011) research shows that adding zinc oxide 3000mg/kg into piglet feed can reduce the death rate of piglets and shorten the treatment time of antibiotic drugs, but reduce the microbial diversity of excrement, while adding amino acid chelated copper can increase the microbial diversity of excrement. However, the application effects of organic copper and organic zinc in the reduction of copper and zinc in pig feed are not consistent. Yang and other researches (2011) show that copper sulfate or copper methionine (the copper content is 125mg/kg) can promote the expression of growth hormone releasing hormone genes, inhibit the expression of somatostatin genes and improve the production performance of piglets, but the source form of copper has no influence on the production performance of growing pigs. This is in substantial agreement with the results of Armstrong et al (2004) in the study of copper citrate in place of copper sulfate. The research data also show that on the premise of not influencing the production performance of pigs, the copper and zinc contents (copper 677.80mg/kg and zinc 2997.35mg/kg) in the manure are still at a higher level even if the organic copper can reduce the copper and zinc addition amount in the pig feed to a certain extent. The organic fertilizer with high copper and zinc contents is returned to the field, and the risk of enriching the copper and zinc elements in the soil is still high.
In the process of pig breeding, copper and zinc in the manure are mainly derived from mineral salt added from feed sources, and the reduction of the feed copper and zinc level from the feed source is the most direct and effective method for reducing the emission of copper and zinc in pig production. However, copper and zinc have various biological functions as essential trace elements and are widely involved in the metabolism and physiological regulation of organisms, and if copper and zinc are reduced blindly, the health of animals is affected and the production performance is reduced. How to scientifically and safely realize the reduction of the feed copper and zinc on the premise of ensuring that the production performance is not reduced is an urgent problem to be solved in the current pig raising production.
Disclosure of Invention
The invention aims to provide a low-copper-zinc environment-friendly biological feed. The patent shows through a series of researches that organic copper and zinc are comprehensively applied to scientific compatibility with biological enzyme, microecologics and the like, comprehensive balance among various trace elements and between the trace elements and other nutrients is realized, the addition of copper and zinc can be effectively reduced from the source of the feed, the excretion of heavy metals such as copper and zinc in excrement is reduced, the microecological balance of intestinal tracts of piglets can be improved, and the production performance is improved, so that the economic benefit and the ecological environmental benefit of pig breeding are well considered.
In order to achieve the purpose, the invention provides the following technical scheme: the low-copper-zinc environment-friendly biological feed comprises a basic feed and a premix, wherein the premix provides the following components for each kilogram of the basic feed:
Cu 40-50mg；
Zn 45-55mg；
proper amount of compound vitamin;
a proper amount of complex enzyme;
a micro-ecological agent;
the microecological formulation provides the following components per kg of basal feed: 0.5X 10 9 -1.5×10 9 cfu/kg lactic acid bacteria, 0.5X 10 10 -1.5×10 10 Clostridium butyricum of cfu/kg, 1X 10 10 -3×10 10 cfu/kg of Bacillus subtilis, 1X 10 10 -3×10 10 cfu/kg of Bacillus licheniformis;
the Cu is in the form of copper amino acid, and the Zn is in the form of zinc amino acid.
In the low-copper-zinc environment-friendly biological feed, the premix also provides the following components for each kilogram of basal feed:
Fe 135-165mg；
Mn 75-95mg；
Se 0.25-0.35mg；
I 0.1-0.2mg。
in the low-copper-zinc environment-friendly biological feed, the compound vitamin provides the following components for each kilogram of basic feed:
VA 1800-2200IU；
VD 3 1350-1650IU；
VE 48-58mg；
VK 3 0.9-1.1mg；
VB 1 5.5-6.5mg；
VB 2 2.5-3.1mg；
VB 6 2.5-3.1mg；
VB 12 0.09-0.11mg；
folic acid 1.8-2.2 mg;
7-9mg of nicotinic acid;
25-31mg of pantothenic acid;
biotin 0.18-0.22 mg.
In the low-copper-zinc environment-friendly biological feed, the complex enzyme provides the following components for each kilogram of basic feed:
phytase 450-;
110-130U/kg of acid protease;
beta-glucanase 45-55U/kg;
xylanase 140-160U/kg.
In the low-copper-zinc environment-friendly biological feed, the Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) with antibacterial and elemental selenium synthesizing capabilities, and the preservation number is as follows: CGMCC No. 24419; the preservation date is as follows: 22/2/2022, depository: china general microbiological culture Collection center, the preservation Address: beijing in China.
Compared with the prior art, the invention has the beneficial effects that:
1. the amino acid copper and zinc is added into the feed to replace inorganic copper and zinc, so that the growth performance of the pigs is improved, and the content of heavy metals such as copper and zinc in excrement can be reduced.
2. The amino acid copper zinc is added into the feed to replace inorganic copper zinc, so that the plasma CEP activity of piglets is increased, the formation of red blood cells, hemoglobin and platelets of piglets is promoted, the SODs activity is improved, the MDA level in plasma is reduced, and the oxidative stress state of piglets is improved.
3. The amino acid copper zinc can be matched with the compound vitamin, the compound enzyme and the microecological preparation to reduce the content of heavy metals such as copper zinc and the like in excrement and improve the growth performance of piglets.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
Influence of daily ration formula on piglet production performance, blood physicochemical index and excrement heavy metal excretion
In order to research the influence of the amino acid copper-zinc chelate (organic copper and organic zinc) instead of inorganic copper sulfate and zinc sulfate on the production performance of piglets, the blood physical and chemical indexes and the excrement heavy metal content, the feasibility of 50 percent of copper-zinc addition in the feed is discussed. Meanwhile, the influence of the addition of microecologics (clostridium butyricum and bacillus licheniformis) in the feed on the copper and zinc reduction effect is researched, and the optimal formula which can take the production performance and the environmental ecological effect into consideration is screened by combining the production performance of piglets and the content of heavy metal in excrement.
1 materials and methods
1.1 Main instruments and devices
Atomic absorption spectroscopy (novAA 350, jena analytical instruments, germany, ltd); fully automatic azotometer (UDK 159, VELP technologies ltd, italy); molokhan oven (SXZ-12-10NP, Shanghai-Heng scientific instruments Co., Ltd.); high temperature digestion furnace (HYP-320, Shanghai fiber inspection instruments, Inc.); an electric heating forced air drying oven (GZX-9140MBE, Shanghai Boxun industries, Ltd.).
The 16S rRNA gene sequence of the SNKM-21 strain of bacillus licheniformis (CGMCC No.24419) related in the embodiment is as follows:
agagtttgatcatggctcaggacgaacgctggcggcgtgcctaatacatgcaagtcgagcggacagatgggagcttgctccctgat gttagcggcggacgggtgagtaacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccggatgcttga ttgaaccgcatggttcaattataaaaggtggcttttagctaccacttacagatggacccgcggcgcattagctagttggtgaggtaacggctc accaaggcaacgatgcgtagccgacctgagagggtgatcggccacactgggactgagacacggcccagactcctacgggaggcagca gtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgagtgatgaaggttttcggatcgtaaaactctgttgttaggga agaacaagtaccgttcgaatagggcggtaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccgcggtaatacg taggtggcaagcgttgtccggaattattgggcgtaaagcgcgcgcaggcggtttcttaagtctgatgtgaaagcccccggctcaaccgggg agggtcattggaaactggggaacttgagtgcagaagaggagagtggaattccacgtgtagcggtgaaatgcgtagagatgtggaggaac accagtggcgaaggcgactctctggtctgtaactgacgctgaggcgcgaaagcgtggggagcgaacaggattagataccctggtagtcc acgccgtaaacgatgagtgctaagtgttagagggtttccgccctttagtgctgcagcaaacgcattaagcactccgcctggggagtacggtc gcaagactgaaactcaaaggaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgaagcaacgcgaagaaccttacca ggtcttgacatcctttgacaaccctagagatagggcttccccttcgggggcagagtgacaggtggtgcatggttgtcgtcagctcgtgtcgtg agatgttgggttaagtcccgcaacgagcgcaacccttgatcttagttgccagcattcagttgggcactctaaggtgactgccggtgacaaac cggaggaaggtggggatgacgtcaaatcatcatgccccttatgacctgggctacacacgtgctacaatgggcagaacaaagggcagcga agccgcgaggctaagccaatcccacaaatctgttctcagttcggatcgcagtctgcaactcgactgcgtgaagctggaatcgctagtaatcg cggatcagcatgccgcggtgaatacgttcccgggccttgtacacaccgcccgtcacaccacgagagtttgtaacacccgaagtcggtgag gtaaccttttggagccagccgccgaaggtgggacagatgattggggtgaagtcgtaacaaggtaacc
the 16S rRNA sequence of the strain SNKM-21 has 99.7 percent of homology with Bacillus licheniformis BCRC 15413(DQ993676.1) and BCRC 12826(EF423608.1) in NCBI GenBank database, and the strain is shown to be Bacillus licheniformis and is named as Bacillus licheniformis SNKM-21.
1.2 test animals and groups
240 healthy 28-day-old duroc long-white large-white (DLY) ternary hybrid piglets (average weight of 6.12kg) are selected in the test, randomly divided into 5 groups according to the weight consistency principle, and fed with different test diets respectively. Each group had 6 replicates, 8 pigs each, for a total of 8 weeks. The conditions of all groups are completely the same except that the groups are fed with different daily rations, and the groups can freely eat and drink water. Animal feeding management is carried out according to a conventional mode, and vaccines are normally inoculated according to a pig farm immunization program in a test period.
1.3 test daily ration
The experimental basal diet was formulated with reference to the nutritional needs of NRC pigs in the united states (2012). The test is divided into two stages of early stage of nursing (1-4 weeks) and late stage of nursing (5-8 weeks), the composition and nutrition level of basic ration in different growth stages are shown in table 1, and the design and treatment of the ration are shown in table 2.
TABLE 1 basic diet formula and nutritional levels
TABLE 2 Experimental design and daily ration treatment
Note: 1 based on the content of copper element; 2 based on the content of zinc element; 3 the copper-free zinc ore is added into each kilogram of feed: zn 60mg, Fe 150.3mg, Mn 85.9mg, Se 0.3mg, I0.14 mg; 4 the compound vitamin is provided for each kilogram of diet: VA 2000IU, VD 3 1500IU,VE 53mg,VK 3 1mg,VB 1 6mg,VB 2 2.8mg,VB 6 2.8mg,VB 12 0.1mg, 2mg folic acid, 8mg nicotinic acid, 28mg pantothenic acid, 0.2mg biotin; 5 the bacillus licheniformis with the capability of resisting bacteria and synthesizing elemental selenium is preserved by Shanghai agricultural academy of sciences, and the China Committee for culture Collection of microorganisms has the preservation number of the common microorganism center: CGMCC No. 24419.
1.4 detection index and method
1.4 Performance properties
The test was weighed on empty stomach in bars at 7 am on weeks 4 and 8 of the trial, and the Average Daily Gain (ADG), feed intake (ADFI) and feed-meat ratio (F/G) were calculated.
1.4.2 sample Collection and Pre-treatment
In the stage of conservation, growth and fattening, animal excrement samples are collected and uniformly mixed for 4 consecutive days from the 28 th day of the experiment, and then the samples are taken, 6 parts of fresh pig excrement are collected in each group, 200g of each part is placed in a disposable sample collection bag to be sealed and frozen at-20 ℃. The feed and fecal samples were sent to the animal husbandry and veterinary institute of academy of agricultural sciences, Shanghai, for testing. The feed and fecal samples were placed in a clean glass petri dish and dried to constant weight in an electric hot air drying oven at 105 ℃. And crushing the dried feed and excrement samples by using a high-speed crusher, sieving the crushed materials by using a 40-mesh sieve, and sealing and storing the sample bags.
1.4.3 determination of copper and Zinc content
The contents of Cu, Zn, Fe and Mn in the feed and the feces are measured by atomic absorption spectrometry according to the determination of the contents of calcium, copper, iron, magnesium, manganese, potassium, sodium and zinc in animal feed (GB/T13885-one 2003). Accurately weighing 3g (accurately to 0.0001g) of feed or excrement sample in a ceramic crucible, carbonizing with small fire on an electric furnace until no smoke exists, and burning in a high-temperature furnace at 550 ℃ for 3 h. The ashed sample is subjected to residue dissolution by 5mL of 6M hydrochloric acid solution, then is transferred to a 100mL volumetric flask without damage, and then is subjected to constant volume, and the contents of copper, zinc, iron and manganese in the sample are measured by an atomic absorption spectrometer (novAA 350, Jena analytical instrument, Germany, Ltd.).
1.4.4 physicochemical indices of blood
On day 28 of the test, the vena cava was sampled and the blood sample was placed in a disposable blood collection tube containing sodium glycinate. The whole blood sample is subjected to conventional blood cell analysis (SK8800, shenzhen shengxikang technologies ltd) to detect White Blood Cells (WBC), Lymphocytes (LYM), intermediate cells (MID), neutrophils (neutrophilic granulocytes, GRAN), Red Blood Cells (RBC), platelet number (PLT) and hemoglobin content (HGB), Hematocrit (HCT), red cell distribution width (RDW), Platelet Distribution Width (PDW), average platelet volume (MPV), large platelet proportion (LCR), etc. in blood.
The levels of porcine Growth Hormone (GH) (G066SC, inter-batch CV < 5.0%, intra-batch CV < 8.3%, sensitivity: >1.0ng/mL), insulin-like growth factor-1 (IGF-1) (I066SC, inter-batch CV < 1.7%, intra-batch CV < 3.5%, sensitivity: >50U/mL) in serum were determined using an ELISA detection kit (Elixir, Canada). Plasma CEP activity was determined using the Ceruloplastin (CEP) activity assay kit (MAK177, Sigma-Aldrich, USA, with an inter-batch CV < 4.6%, an intra-batch CV < 6.0%, sensitivity: >1.0 pg/mL). Detecting the activity of total SOD (T-SOD), copper-zinc SOD (CuZn-SOD) and manganese SOD (Mn-SOD) in plasma by adopting a superoxide dismutase (SOD) parting kit (A001-2, Nanjing creates a bioengineering research institute, the inter-batch CV is less than 5.0%, the intra-batch CV is less than 8.3%, and the sensitivity is more than 1.0 ng/mL); malondialdehyde (MDA) kit (A003-4-1, Nanjing, institute of bioengineering, with inter-batch CV of less than 4.1%, intra-batch CV of less than 3.5%, and sensitivity of > 0.5nmol/mL) is used.
2 analysis of results
The results of piglet production performance measurements (table 3) show that when bacillus licheniformis stored in the academy of agricultural sciences of Shanghai is added, 45mg/kg of copper glycinate and 50mg/kg of zinc glycinate (group C, the addition amount of copper and zinc is reduced by 50%) are used for replacing 90m/kg of copper sulfate and 100mg/kg of zinc sulfate (group B), piglet weight gain, feed intake and feed conversion ratio are all remarkably improved (P is less than 0.05), production performance is remarkably superior to that of a commercial bacillus licheniformis group (group E) and an organic copper zinc group (group D) without clostridium butyricum and bacillus licheniformis (group P is less than 0.05), and production performance of a blank control group (group A) without premix is the worst and is remarkably lower than that of other groups (P is less than 0.05).
TABLE 3 Effect of the formula composition of the ration on piglet Productivity
Note: the same row data with different lower case letters indicates that the difference reaches a significant level (P <0.05), and with the same lower case letters indicates that the difference is not significant (P >0.05), the same below.
One of the important biological functions of copper is the participation in iron metabolism of the body and the participation of iron together in the production of erythrocytes and hemoglobin in the body. As can be seen from table 4, the feed plus the cu-zn containing premix significantly increased RBC, HGB and HCT in the blood of piglets compared to the blank control group, with group C being the highest and the organic cu-zn group (C, D, E group) being significantly higher than the inorganic cu-zn group (group B) (P < 0.05). The result shows that the organic copper zinc has more advantages in the aspects of improving the generation of piglet red blood cells and blood proteins than the inorganic copper zinc, and the feed is added with the clostridium butyricum and the bacillus licheniformis simultaneously to further increase the advantages of the organic copper zinc.
Growth axis hormones GH and IGF-1 play important regulatory roles in animal growth and development. The detection result shows that the levels of GH and IGF-1 in the plasma of the group C are obviously higher than those of the commercial bacillus licheniformis (group D) and the clostridium butyricum-free bacillus licheniformis (group E), and the result shows that the combination of the clostridium butyricum and bacillus licheniformis SNKM-2124419 and the glycine copper-zinc chelate in the feed can further promote the secretion of GH and IGF-1 so as to improve the piglet production performance to a greater extent.
TABLE 4 influence of daily ration formula composition on physical and chemical indicators of piglet blood
SOD is an important member of an organism antioxidant system, CEP not only has antioxidant function, but also participates in the conversion of iron valence state in vivo, thereby having important regulation and control function in iron metabolism. Therefore, the activity of CEP and SOD (especially CuZn-SOD) is an important index for reflecting the copper nutrition status of organisms and the biological potency of feed copper. The concentrations of SOD and MDA are key indicators of the antioxidant state of the reactor. As can be seen from Table 5, the CEP, SOD and CuZn-SOD activities of the group C plasma are significantly higher than those of other groups, and the MDA concentration is significantly lower than that of other groups (P is less than 0.05), which indicates that the formula combination of the group C daily ration is beneficial to improving the oxidation resistance of the organism, thereby improving the oxidative stress state of the organism.
TABLE 5 influence of daily ration formula composition on piglet blood antioxidant index
The detection results (table 6) of the copper and zinc contents in the excrement show that the copper and zinc contents in the excrement of piglets can be remarkably reduced by replacing inorganic copper sulfate and zinc sulfate with 50% of glycine copper-zinc chelate, the copper and zinc contents in the excrement of group C are respectively reduced by 43.41% and 42.62% compared with those of group B (P <0.05), and no remarkable difference exists between organic copper and zinc groups (P > 0.05).
TABLE 6 influence of daily ration formulation on the heavy metal content in piglet feces
3 conclusion
The glycine copper-zinc chelate with 50 percent of reduction amount is used for replacing inorganic copper sulfate and zinc sulfate, so that the piglet production performance is improved, the hemoglobin generation and the oxidative stress state are improved, and the copper and zinc contents in the piglet excrement are obviously reduced. The better effect can be obtained by combining the glycine copper zinc chelate, the clostridium butyricum and the bacillus licheniformis SNKM-2124419.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Sequence listing
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<120> low-copper-zinc environment-friendly biological feed
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agagtttgat catggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgagc 60
ggacagatgg gagcttgctc cctgatgtta gcggcggacg ggtgagtaac acgtgggtaa 120
cctgcctgta agactgggat aactccggga aaccggggct aataccggat gcttgattga 180
accgcatggt tcaattataa aaggtggctt ttagctacca cttacagatg gacccgcggc 240
gcattagcta gttggtgagg taacggctca ccaaggcaac gatgcgtagc cgacctgaga 300
gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag gcagcagtag 360
ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc cgcgtgagtg atgaaggttt 420
tcggatcgta aaactctgtt gttagggaag aacaagtacc gttcgaatag ggcggtacct 480
tgacggtacc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 540
ggtggcaagc gttgtccgga attattgggc gtaaagcgcg cgcaggcggt ttcttaagtc 600
tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggga acttgagtgc 660
agaagaggag agtggaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaacac 720
cagtggcgaa ggcgactctc tggtctgtaa ctgacgctga ggcgcgaaag cgtggggagc 780
gaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttagagg 840
gtttccgccc tttagtgctg cagcaaacgc attaagcact ccgcctgggg agtacggtcg 900
caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 960
attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgacaac cctagagata 1020
gggcttcccc ttcgggggca gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1080
gagatgttgg gttaagtccc gcaacgagcg caacccttga tcttagttgc cagcattcag 1140
ttgggcactc taaggtgact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1200
catcatgccc cttatgacct gggctacaca cgtgctacaa tgggcagaac aaagggcagc 1260
gaagccgcga ggctaagcca atcccacaaa tctgttctca gttcggatcg cagtctgcaa 1320
ctcgactgcg tgaagctgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg 1380
ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1440
tgaggtaacc ttttggagcc agccgccgaa ggtgggacag atgattgggg tgaagtcgta 1500
acaaggtaac c 1511
Claims (5)
1. The low-copper-zinc environment-friendly biological feed comprises a basic feed and a premix, and is characterized in that the premix provides the following components for each kilogram of the basic feed:
Cu 40-50mg；
Zn 45-55mg；
proper amount of compound vitamin;
a proper amount of complex enzyme;
a micro-ecological agent;
the micro-ecological preparation provides the following components for each kilogram of basic feed: 0.5X 10 9 -1.5×10 9 cfu/kg lactic acid bacteria, 0.5X 10 10 -1.5×10 10 Clostridia butyricum of cfu/kg, 1X 10 10 -3×10 10 cfu/kg of Bacillus subtilis, 1X 10 10 -3×10 10 cfu/kg of Bacillus licheniformis;
the Cu is in the form of copper amino acid, and the Zn is in the form of zinc amino acid.
2. The low-copper-zinc environment-friendly biological feed as claimed in claim 1, which is characterized in that: the premix also provides the following components per kilogram of basal feed:
Fe 135-165mg；
Mn 75-95mg；
Se 0.25-0.35mg；
I 0.1-0.2mg。
3. the low-copper-zinc environment-friendly biological feed as claimed in claim 1, which is characterized in that: the compound vitamin provides the following components for each kilogram of basic feed:
VA 1800-2200IU；
VD 3 1350-1650IU；
VE 48-58mg；
VK 3 0.9-1.1mg；
VB 1 5.5-6.5mg；
VB 2 2.5-3.1mg；
VB 6 2.5-3.1mg；
VB 12 0.09-0.11mg；
folic acid 1.8-2.2 mg;
7-9mg of nicotinic acid;
25-31mg of pantothenic acid;
biotin 0.18-0.22 mg.
4. The low-copper-zinc environment-friendly biological feed as claimed in claim 1, which is characterized in that: the compound enzyme provides the following components for each kilogram of basic feed:
phytase 450-;
110-130U/kg of acid protease;
beta-glucanase 45-55U/kg;
xylanase 140-160U/kg.
5. The low-copper-zinc environment-friendly biological feed as claimed in claim 1, which is characterized in that: the Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) with antibacterial and elemental selenium synthesis capability, and the preservation number is as follows: CGMCC No. 24419; the preservation date is as follows: 22/2/2022, depository: china general microbiological culture Collection center (CGMCC).
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| CN202210392054.1A CN115104675A (en) | 2022-04-14 | 2022-04-14 | Low-copper-zinc environment-friendly biological feed |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550866A (en) * | 2012-01-06 | 2012-07-11 | 福建省莆田市优利可农牧发展有限公司 | Pig feed |
| CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
| CN104982672A (en) * | 2015-03-04 | 2015-10-21 | 江苏财经职业技术学院 | Ecological and environment-friendly piglet feed |
| CN106479927A (en) * | 2016-11-02 | 2017-03-08 | 中国农业大学 | Method and its application using Bacillus licheniformis biosynthesiss nanometer selenium |
| CN106490369A (en) * | 2016-12-14 | 2017-03-15 | 深圳市前海金卓生物技术有限公司 | Pig starter feed core material |
-
2022
- 2022-04-14 CN CN202210392054.1A patent/CN115104675A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550866A (en) * | 2012-01-06 | 2012-07-11 | 福建省莆田市优利可农牧发展有限公司 | Pig feed |
| CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
| CN104982672A (en) * | 2015-03-04 | 2015-10-21 | 江苏财经职业技术学院 | Ecological and environment-friendly piglet feed |
| CN106479927A (en) * | 2016-11-02 | 2017-03-08 | 中国农业大学 | Method and its application using Bacillus licheniformis biosynthesiss nanometer selenium |
| CN106490369A (en) * | 2016-12-14 | 2017-03-15 | 深圳市前海金卓生物技术有限公司 | Pig starter feed core material |
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