CN115093982B - Pseudomonas ZB30 and application thereof in styrene waste gas degradation - Google Patents
Pseudomonas ZB30 and application thereof in styrene waste gas degradation Download PDFInfo
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/346—Controlling the process
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses pseudomonas ZB30 and application thereof in styrene waste gas degradation. The Pseudomonas sp fungal "ZB30", accession number: cctccc No. M2021371. Use of pseudomonas fungus ZB30 in styrene waste gas degradation. The abundance of ZB30 fungi in the bioactive filler is artificially increased, so that the removal efficiency of the bioactive filler on styrene-containing waste gas can be improved. After the reactor added with the pseudomonas ZB30 enters a stable period, the absorption and removal efficiency of the reactor for the waste gas containing styrene is obviously improved. The active filler after adding the pseudomonas ZB30 can shorten the start-up time of the reactor of the biotrickling filter.
Description
Technical Field
The invention belongs to the field of environmental pollution control, and relates to Pseudomonas sp bacteria ZB30 and application thereof in biodegradation of styrene waste gas.
Background
Atmospheric pollution is one of the main problems of environmental pollution and is also a hot spot problem of current concern. Along with the development of social economy, the industrial development is also faster and faster. The atmospheric pollution caused by industrial emission is more serious, and a large amount of malodorous waste gas such as styrene is generated in the actual industrial production process. Styrene belongs to volatile organic compounds and is mainly produced by industries such as plastics, paint, coating, fine chemical industry and the like. Styrene is taken as a characteristic factor of malodorous waste gas in GB14554-93 malodorous substance emission standard formulated in China. The emission of styrene waste gas not only pollutes soil, atmosphere and water resources, but also causes great potential harm to physical and mental health of human beings. Styrene can invade human body through various ways such as respiratory system, skin and gastrointestinal tract, and causes harm to respiratory tract mucous membrane, nervous system, liver and lung. The light styrene can cause sore throat, nasal discharge, cough and the like of a human body, and the heavy styrene can cause acute poisoning, and symptoms such as vomit, nausea, debilitation, dizziness, anorexia, abdominal distention, depression and the like appear. Therefore, the treatment of styrene offgases is of great significance to the environment and human health. Along with the continuous improvement of living standard, the environmental protection consciousness of people is continuously improved, the requirements on living quality and environmental conditions are also higher and higher, and how to efficiently treat the styrene waste gas is a focus of attention.
The styrene offgas treatment method can be classified into three types: physical, chemical and biological methods. Wherein the physical method comprises adsorption method, membrane separation method, absorption method, etc.; chemical methods include photocatalytic methods, thermal destruction methods, and the like; the biological method mainly comprises a biological trickling filtration method, a biological washing method, a biological filter method and the like. Although both physical and chemical methods have many advantages, there are also many disadvantages such as high investment cost, complicated operation, and easy secondary pollution. Compared with the traditional treatment method, the biological method for treating the waste gas has the advantages of simple equipment requirement, low operation cost, difficult secondary pollution, good safety, high treatment efficiency and the like. One of the important methods for treating styrene waste gas by adopting a biological method is to screen out strains with the capability of efficiently degrading styrene waste gas, and the method has become a main method for treating malodorous waste gas at home and abroad. At present, few researches on microbial degradation of styrene waste gas are reported.
Disclosure of Invention
It is an object of the present invention to provide a Pseudomonas sp bacteria "ZB30".
The invention is realized by the following technical scheme:
the Pseudomonas sp bacteria ZB30 provided by the invention are preserved in China center for type culture Collection, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021371, date of preservation: 2021, 4 and 14.
The biological characteristics of this strain are as follows: near circular, regular edge, pale yellowish white, smooth surface, gram negative.
The invention relates to a screening technical scheme of strains, which comprises the following steps:
the provided strain is obtained by screening activated sludge in a biotrickling filter reactor of an industrial enterprise. 10g of acclimated bioreactor sludge is taken, and three different concentration gradients of 5 times, 50 times and 500 times of double distilled water are respectively coated on a PDA bacterial solid culture medium. Each petri dish was coated with 2mL of diluted sludge solution, incubated at 32 ℃ for 2 days in an incubator, mycelia were picked from the edges of colonies, transferred to a fresh PDA plate for streaking, isolated culture, repeated until a pure culture was obtained, and transferred to PDA slant for preservation.
The 16S rDNA sequence of the Pseudomonas sp bacteria "ZB30" is as follows, SEQ ID NO.1. The homology with Pseudomonas bacteria (GeneBank accession number: MN 410633) was highest as shown by BLAST search in NCBI, and was 100.0%, so that the strain was identified as Pseudomonas (Pseudomonas sp.) bacteria.
SEQ ID NO.1：
CAGTCGAGCGGCAGCGGGTCCTTCGGGATGCCGGCGAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGGTAGTGGGGGATAACTCGGGGAAACTCGAGCTAATACCGCATACGTCCTACGGGAGAAAGCGGGGGATCTTCGGACCTCGCGCTACCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGAGGTAAAGGCTCACCAAGGCTACGATCCGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAaGAAGgTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGCTAATACCtTGCTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGATAAGTTGGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATCCAAAACTGTCTGACTAGAGTATGGCAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGGCTAATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGATCCTTGAGATCTTAGTGGCGCAGCTAACGCATTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGACACAGgTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTTAAGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACCTC
It is another object of the present invention to provide the use of the above-mentioned Pseudomonas sp bacteria "ZB30" for the degradation of styrene-containing waste gases.
Preferably, the use of a bacterium of the genus Pseudomonas "ZB30" for the degradation purification of styrene offgases in a biotrickling filter reactor.
The biological trickling filtration tower reactor has the advantages of 8000mm tower height, 1500mm tower diameter, 1200mm filler height and 3000m exhaust gas flow 3 And/h. Spray density of 10m 3 /(m 2 H) residence time of 12s. Injecting Pseudomonas bacteria "ZB30" into the substrate, wherein the concentration of the bacteria "ZB30" in the substrate is not less than 3.5X10% 7 CFU/g. The matrix composition is: the mixture of the activated carbon, the ceramsite and the wood chips comprises the following components in percentage by mass: 1:2.
a further object of the present invention is to provide a method for degrading styrene offgas by introducing styrene offgas into a biotrickling filter reactor having a packing of not less than 3.5X10 per gram of matrix 7 CFU/g of the bacterium "ZB30" of Pseudomonas sp.
Preservation description:
the Pseudomonas sp bacteria ZB30 provided by the invention are preserved in China center for type culture Collection, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021371, date of preservation: 2021, 4 and 14.
The invention has the beneficial effects that:
(1) The pseudomonas bacteria ZB30 can reduce the styrene concentration in malodorous waste gas and has excellent treatment effect.
(2) The domestication time of the active filler added with the pseudomonas bacterial strain ZB30 is obviously shortened, and the biotrickling filter reactor can be started quickly;
(3) The method for degrading the styrene waste gas by the pseudomonas bacteria ZB30 provided by the invention is simple to operate;
(4) The Pseudomonas strain ZB30 strain provided by the invention can be stored for a long time, can be continuously used after being activated for one year in an ultralow temperature refrigerator at-80 ℃, has no obvious reduction on the removal efficiency of styrene-containing waste gas, and has good storability.
Drawings
FIG. 1 is a schematic diagram of a bioreactor structure;
FIG. 2 is a colony morphology of the strain;
FIG. 3 is a phylogenetic tree diagram.
Detailed Description
The present invention will be further described in detail with reference to the following examples in order to make the objects, techniques and features of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1: isolated culture of Pseudomonas bacteria "ZB30
The provided strain is obtained by screening activated sludge from a biotrickling filter reactor (shown in figure 1) of an industrial enterprise. 10g of acclimated bioreactor sludge is taken, and three different concentration gradients of 5 times, 50 times and 500 times of double distilled water are respectively coated on a PDA bacterial solid culture medium. Each petri dish was coated with 2mL of diluted sludge solution, incubated at 32 ℃ for 2 days in an incubator, mycelia were picked from the edges of colonies, transferred to a fresh PDA plate for streaking, isolated culture, repeated until a pure culture was obtained, and transferred to PDA slant for preservation.
Example 2: morphological and molecular biological characterization of Pseudomonas bacteria "ZB30
According to the handbook of bacteria identification handbook, the morphological characteristics of Pseudomonas bacteria ZB30 are observed under a microscope, specifically, the selected strain is inoculated on a PDA flat plate by adopting a dibbling method, and is cultured at the constant temperature of 32 ℃ to visually observe the morphological characteristics of the strain, including the characteristics of colony morphology, color, size, edge characteristics, hypha character, growth speed and the like.
The morphological characteristics of the Pseudomonas bacteria "ZB30" of the invention are as follows:
as shown in FIG. 2, the Pseudomonas bacteria "ZB30" strain was on PDA medium, which was nearly round, regular in edge, pale yellowish white, smooth in surface, and gram negative.
Example 3: molecular biological identification of Pseudomonas bacteria "ZB30
1. Genomic DNA extraction
The genomic DNA extraction method is as follows: selecting 2mL of bacterial liquid, centrifuging for 3 minutes at the temperature of 4 ℃ and the speed of 12000rpm, and collecting and obtaining bacterial cells; adding 600 μl of 2×CTAB (containing 2% beta-mercaptoethanol), quick freezing in liquid nitrogen for 1 min, transferring to water bath at 64deg.C for 1 min, repeating the above process for 3 times, and shaking at high speed for 2min, and water bath at 64deg.C for 30 min; an equal volume of chloroform was added: isoamyl alcohol (volume ratio of 24:1), mixing the mixture upside down, standing on ice for 3 minutes, centrifuging at 4 ℃ and 12000rpm for 15 minutes, and taking the supernatant to a new centrifuge tube; adding equal volume of isopropanol into the supernatant, mixing gently upside down, standing on ice for 30 min, and centrifuging at 4deg.C and 12000rpm for 5 min; the supernatant was discarded, the precipitate was washed with 75% absolute ethanol, and then, bacterial DNA was obtained by air-drying at room temperature, followed by ddH 2 O dissolves the DNA precipitate and the precipitate is stored in a refrigerator for later use.
2. Pseudomonas bacteria ZB30 ITS-PCR amplification and molecular identification
The Internal Transcribed Spacer (ITS) of rRNA genes of bacterial genome is amplified by PCR technique using bacterial universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3') as PCR amplification primers. PCR reaction System (20. Mu.L): 2X Taq PCR MasterMix. Mu.L, 1. Mu.L of the upstream primer (10. Mu. Mol/L), 1. Mu.L of the downstream primer (10. Mu. Mol/L), 1. Mu.L of the DNA template (50 ng/L), and ddH were used 2 O was made up to a volume of 20. Mu.L. Then, PCR amplification was performed on a PCR apparatus under the following conditions: pre-denaturation at 94 ℃ for 5 min; 32 cycles: denaturation at 94℃for 50 seconds, annealing at 58℃for 50 seconds, extension at 72℃for 1 minute for 30 seconds; further extension was carried out at 72℃for 10 minutes. Finally, the PCR product was purified and then subjected to sample sequencing.
The nucleotide sequence obtained by sequencing was subjected to BLAST alignment in NCBI, and the alignment showed that the homology with Pseudomonas bacteria "ZB30" (GenBank accession number MN 410633) was the highest, and thus the strain was identified as Pseudomonas bacteria.
Example 4: phylogenetic analysis of Pseudomonas bacteria "ZB30
BLAST searches of the Pseudomonas bacteria "ZB30" sequences obtained above in NCBI were performed, and known strain ITS sequences similar thereto were downloaded for constructing phylogenetic tree. Multiple sequence alignment was performed using clustalx1.83, the alignment was performed using MEGA7.0 software and using Neighbor training statistical methods, bootstrap values were set to 1000, and a Tamura-Nei model base substitution pattern was used to construct phylogenetic trees based on rDNA ITS sequences. A specific phylogenetic tree is shown in fig. 3.
Example 5: pseudomonas bacteria ZB30 fermentation and filler preparation
After the culture and purification by PDA solid culture medium, the Pseudomonas bacteria ZB30 are inoculated to the fermentation culture medium, and the amplification culture is carried out according to the volume ratio of the seed solution to the fermentation culture medium of 1:9. The fermentation medium is Tryton 10g/L, yeast extract 5g/L, sodium chloride 10g/L, ddH is used 2 And (3) preparing O. The temperature of fermentation culture is 32-37 ℃, the dissolved oxygen is more than 1.6mg/L, the pressure is 0.08MPa, and the culture time is 48 hours. And (3) placing the fermentation liquor in a refrigerated centrifuge, centrifuging at 4 ℃ and 4000rpm for 30 minutes, removing supernatant, collecting precipitated thalli, and re-suspending the thalli by using fresh sterile culture solution to obtain bacterial suspension with the final concentration of 0.8-1.0 g/L. The "ZB30" suspension was adsorbed onto the filler to complete the preparation of the filler enriched in Pseudomonas bacteria "ZB30".
Example 6: pseudomonas strain ZB30 improves the absorption and removal efficiency of the biological trickling filtration reactor on the waste gas containing styrene
Two sets of parallel biological trickling filtration tower reactors (a control group and a treatment group) are designed, the original activated sludge and the activated sludge obtained by the bacterial inoculation obtained by the embodiment are respectively adopted, and the difference of the absorption and removal efficiencies of two sets of equipment on ammonia-containing waste gas in one working day period is detected. After two sets of equipment are selected and started for 10 days, sampling is performed to determine the required parameters. The styrene-containing concentration of the waste gas at the input port of the reactor of the biotrickling filter is 150mg/m respectively 3 、300mg/m 3 、450mg/m 3 、600mg/m 3 And 750mg/m 3 And measuring the degradation rate of the styrene. The measurement results are shown in Table 1. As can be seen from the data results of Table 1, the processThe cleaning efficiency of the group biological trickling filtration tower reactor to the waste gas containing styrene is between 98.5 percent and 99.8 percent, which is obviously higher than that of the control group.
Example 7: pseudomonas bacteria "ZB30" shorten the start-up time of a biotrickling filter reactor
Two parallel biological trickling filter reactors of a control group and a treatment group are designed, and original activated sludge and activated sludge obtained by artificially adding bacteria obtained by the embodiment are respectively adopted to detect the removal efficiency of waste gas containing styrene from the first day to the 10 th day after the start. Sampling time is fixed to 8 points in the morning every day, exhaust gas samples of an input port and an output port are extracted, and ammonia gas content is measured in mg/m 3 . The measured data are shown in Table 2.
The results in Table 2 show that the biotrickling filter reactors of the treatment group have reached 97.6% efficiency from day 6 to day 10, and 99.8% efficiency; whereas the control biotrickling filter reactor reached 86.5% efficiency from day 6 and then gradually became stable. The data statistics of Table 2 show that Pseudomonas bacteria "ZB30" can greatly shorten the start-up time of the biotrickling filter reactor.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the particular embodiments disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Sequence listing
<110> Hangzhou university of education
<120> Pseudomonas ZB30 and its use in styrene waste gas degradation
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1388
<212> DNA
<213> ZB30
<400> 1
cagtcgagcg gcagcgggtc cttcgggatg ccggcgagcg gcggacgggt gagtaatgcc 60
taggaatctg cctggtagtg ggggataact cggggaaact cgagctaata ccgcatacgt 120
cctacgggag aaagcggggg atcttcggac ctcgcgctac cagatgagcc taggtcggat 180
tagctagttg gtgaggtaaa ggctcaccaa ggctacgatc cgtagctggt ctgagaggat 240
gatcagccac actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa 300
tattggacaa tgggcgaaag cctgatccag ccatgccgcg tgtgtgaaga aggtcttcgg 360
attgtaaagc actttaagtt gggaggaagg gcagtaagct aataccttgc tgttttgacg 420
ttaccgacag aataagcacc ggctaacttc gtgccagcag ccgcggtaat acgaagggtg 480
caagcgttaa tcggaattac tgggcgtaaa gcgcgcgtag gtggtttgat aagttggatg 540
tgaaagcccc gggctcaacc tgggaattgc atccaaaact gtctgactag agtatggcag 600
agggtggtgg aatttcctgt gtagcggtga aatgcgtaga tataggaagg aacaccagtg 660
gcgaaggcga ccacctgggc taatactgac actgaggtgc gaaagcgtgg ggagcaaaca 720
ggattagata ccctggtagt ccacgccgta aacgatgtcg actagccgtt gggatccttg 780
agatcttagt ggcgcagcta acgcattaag tcgaccgcct ggggagtacg gccgcaaggt 840
taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 900
agcaacgcga agaaccttac caggccttga catgcagaga actttccaga gatggattgg 960
tgccttcggg aactctgaca caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg 1020
ttgggttaag tcccgtaacg agcgcaaccc ttgtccttag ttaccagcac gttaaggtgg 1080
gcactctaag gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagtcatc 1140
atggccctta cggcctgggc tacacacgtg ctacaatggt cggtacaaag ggttgccaag 1200
ccgcgaggtg gagctaatcc cataaaaccg atcgtagtcc ggatcgcagt ctgcaactcg 1260
actgcgtgaa gtcggaatcg ctagtaatcg tgaatcagaa tgtcacggtg aatacgttcc 1320
cgggccttgt acacaccgcc cgtcacacca tgggagtggg ttgctccaga agtagctagt 1380
ctaacctc 1388
Claims (9)
1. A bacterial strain ZB30, characterized by being classified as PseudomonasPseudomonassp. deposited in China center for type culture Collection, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021371, date of preservation: 2021, 4, 14; the biological characteristics are as follows: nearly round, regular edges, pale yellow-white, smooth surface, gram negative; ITS rDNA ITS sequence is shown in SEQ ID NO: 1.
2. Use of a pseudomonas strain ZB30 according to claim 1 for the degradation of styrene-containing waste gases.
3. The use according to claim 2, in the degradation purification of styrene-containing waste gases in a biotrickling filter reactor.
4. The process according to claim 3, wherein the filler in the biotrickling filter reactor contains not less than 3.5X10 of filler per gram of matrix 7 CFU/g of Pseudomonas as claimed in claim 1Pseudomonassp, strain ZB30.
5. The use according to claim 4, wherein the matrix is a mixture of activated carbon, ceramsite and wood chips, and the mass ratio is 2:1:2.
6. the use according to claim 3, wherein the operating parameters of the biotrickling filter reactor: tower height 8000mm, tower diameter 1500mm, packing height 1200mm, exhaust gas flow 3000m 3 Spray density of 10m 3 /（m 2 H) residence time of 12s.
7. A styrene waste gas degradation method is characterized in that styrene waste gas is introduced into a biotrickling filter reactor, and the filler in the biotrickling filter reactor is not less than 3.5 multiplied by 10 per gram of matrix 7 CFU/g of Pseudomonas as claimed in claim 1Pseudomonassp, strain ZB30.
8. The method of claim 7, wherein the matrix is a mixture of activated carbon, ceramsite and wood chips, and the mass ratio of the matrix is 2:1:2.
9. the method of claim 7, wherein the operating parameters of the biotrickling filter reactor: tower height 8000mm, tower diameter 1500mm, packing height 1200mm, exhaust gas flow 3000m 3 Spray density of 10m 3 /（m 2 H) residence time of 12s.
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