CN114015582B - Microbacterium ZB21 and application thereof in trimethylamine waste gas degradation - Google Patents
Microbacterium ZB21 and application thereof in trimethylamine waste gas degradation Download PDFInfo
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- CN114015582B CN114015582B CN202111321245.0A CN202111321245A CN114015582B CN 114015582 B CN114015582 B CN 114015582B CN 202111321245 A CN202111321245 A CN 202111321245A CN 114015582 B CN114015582 B CN 114015582B
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- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 239000002912 waste gas Substances 0.000 title claims abstract description 30
- 241001467578 Microbacterium Species 0.000 title claims abstract description 28
- 230000015556 catabolic process Effects 0.000 title claims abstract description 11
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 11
- 239000000945 filler Substances 0.000 claims abstract description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 13
- 230000001580 bacterial effect Effects 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 19
- 238000000855 fermentation Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 11
- 239000011159 matrix material Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
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- 239000007789 gas Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
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- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 239000002068 microbial inoculum Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 2
- 239000002023 wood Substances 0.000 claims 1
- 241000168413 Exiguobacterium sp. Species 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 230000000975 bioactive effect Effects 0.000 abstract 2
- 241000194103 Bacillus pumilus Species 0.000 abstract 1
- 239000010802 sludge Substances 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 108091023242 Internal transcribed spacer Proteins 0.000 description 4
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- FYGHSUNMUKGBRK-UHFFFAOYSA-N 1,2,3-trimethylbenzene Chemical compound CC1=CC=CC(C)=C1C FYGHSUNMUKGBRK-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
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- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001468125 Exiguobacterium Species 0.000 description 1
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- 150000001412 amines Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
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- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention relates to a bacillus pumilus ZB21 and application thereof in trimethylamine waste gas degradation. The invention provides a fungus ZB21 of the genus Microbacterium (Exiguobacterium sp.) with deposit number: cctccc No. M2021370. Use of fungus ZB21 in trimethylamine waste gas degradation. The abundance of ZB21 fungi in the bioactive filler is artificially increased, so that the removal efficiency of the bioactive filler on trimethylamine-containing waste gas can be improved. After the reactor added with the microbacterium ZB21 enters a stable period, the absorption and removal efficiency of the waste gas containing trimethylamine is obviously improved. The active filler after adding the micro bacillus ZB21 can shorten the start-up time of the reactor of the biotrickling filter.
Description
Technical Field
The technology belongs to the field of environmental pollution treatment, and relates to a strain of micro bacillus (Exiguobacterium sp.) bacteria ZB21 and application thereof in trimethylamine waste gas biodegradation.
Background
Atmospheric pollution is a topic of concern and one of the main problems of environmental pollution. Along with the development of social economy, the industrial development is also faster and faster. With this, the atmospheric pollution caused by the emission of industrial exhaust gas is more and more serious, and a large amount of malodorous waste gas such as trimethylamine is generated in the actual industrial production process. Trimethylamine is one of the main representatives of organic amine waste gas pollutants, and has large emission and low odor threshold value, so that the trimethylamine causes serious malodorous pollution to the atmospheric environment. The emission of trimethylamine malodorous waste gas has caused serious harm to the physical health and ecological environment of residents. Thus, the abatement of trimethylamine exhaust gas has become a very urgent issue for atmospheric pollution control.
The traditional method for treating malodorous waste gas such as trimethylamine is a physical and chemical method, but the methods have the defects of high operation cost, easiness in secondary pollution and the like. Compared with the traditional physicochemical method, the biological method has the advantages of low energy consumption, low cost, difficult secondary pollution and the like, and is gradually becoming the main method for treating malodorous waste gas at home and abroad at present. At present, many researches on biological treatment of inorganic malodors such as ammonia and hydrogen sulfide are reported, but few researches on biological treatment of organic malodors such as trimethylamine are reported.
The adoption of the biological trickling filter is a relatively popular biological method for treating industrial malodorous waste gas at present. The solid packed bed is arranged in the reactor of the biotrickling filter for culturing microorganisms, the microorganisms are attached and grown on the surface of the solid packing to form firm biological membranes, and the waste gas flows through the bioreactor, and harmful substances are adsorbed on the biological membranes and then degraded by the microorganisms. In order to reduce the debugging time of the biotrickling filter, special microorganisms are required to be inoculated into the bottom mud in the starting stage of the malodorous waste gas removal reactor. And (3) screening high-efficiency bacteria capable of absorbing and degrading trimethylamine from the sludge at the bottom of the biotrickling filter, culturing in a manual mode, and adding the bacteria into the biotrickling filter reactor to further improve the biotrickling filter. In addition, the high-efficiency bacteria obtained by screening lays an important foundation for researching the biodegradation mechanism of the trimethylamine-containing waste gas.
Disclosure of Invention
It is an object of the present invention to provide a bacterium ZB21 of the genus Microbacterium (Exiguobacterium sp.) in view of the deficiencies of the prior art.
The invention is realized by the following technical scheme:
the invention provides a method for preserving Microbacterium (Exiguobacterium sp.) bacteria ZB21 in China center for type culture Collection, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021370, date of preservation: 2021, 4 and 21.
The biological characteristics of this strain are as follows: the strain is gram-positive bacillus, and the colony is round, has a diameter of 1.8-3.6mm, is yellowish white and has a smooth surface.
Bacterial ZB21 strain is obtained by screening activated sludge in a biotrickling filter reactor of an industrial enterprise.
A second object of the present invention is to provide the use of the bacterium ZB21 described above for the degradation of trimethylamine-containing waste gases.
Preferably, the bacillus ZB21 is applied to degradation and purification of trimethylamine waste gas in a biotrickling filter reactor.
Preferably, the filler in the biotrickling filter reactor adopts a matrix and bacteria ZB21, wherein each gram of the matrix contains not less than 4.0x10 7 Bacterial ZB21 of CFU, the matrix is activated carbon, haydite and saw dust according to 1:1:2, and a mass ratio of the mixture.
Preferably, the operating setting parameters of the biotrickling filter reactor are as follows: tower height 8000mm, tower diameter 1500mm, packing height 1200mm, exhaust gas flow 3000m 3 /h; spray density of 12m 3 /(m 2 H) residence time of 10s.
A third object of the present invention is to provide a fermented product which is a fermentation broth or liquid microbial inoculum of Microbacterium ZB21.
Preferably, the preparation method of the fermentation broth of the bacillus bacteria ZB21 specifically comprises the following steps:
inoculating the purified Microbacterium ZB21 cultured by the PDA solid culture medium to a fermentation culture medium, and performing amplification culture according to the volume ratio of the seed solution to the fermentation culture medium of 1:9; wherein the temperature of fermentation culture is 32-37 ℃, the dissolved oxygen is more than 1.8mg/L, the pressure is 0.08MPa, and the culture time is 60 hours.
The fermentation culture medium is yeast powder 10.0g/L, peptone 10.0g/L, naCl 8.0.0 g/L and agar 15.0g/L.
Preferably, the preparation method of the liquid microbial agent of the bacillus ZB21 specifically comprises the following steps:
and (3) placing the fermentation liquor in an ultralow temperature centrifugal machine at 4 ℃, centrifuging at 4000rpm for 15min, collecting precipitated thalli after removing supernatant, and re-suspending thalli by using fresh sterile culture liquor to obtain the liquid microbial inoculum with the final bacterial concentration of 0.5-0.8 g/L. The fermentation culture temperature is 32-37 ℃, the dissolved oxygen is more than 1.8mg/L, the pressure is 0.08MPa, and the culture time is 60 hours.
The fermentation culture medium is yeast powder 10.0g/L, peptone 10.0g/L, naCl 8.0.0 g/L and agar 15.0g/L.
Preservation description:
the bacterial strain ZB21 of the genus Microbacterium (Exiguobacterium sp.) of the present invention is deposited in China center for type culture Collection, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021370, date of preservation: 2021, 4 and 21.
The invention has the beneficial effects that:
(1) The trimethylamine concentration in malodorous waste gas can be obviously reduced by using the screened micro bacillus bacterial strain ZB21, and the treatment effect is excellent;
(2) The domestication time of the active filler added with the bacillus micro strain ZB21 is obviously shortened, and the biotrickling filter reactor can be started quickly;
(3) The application method of the microbacterium bacterial strain ZB21 in trimethylamine waste gas degradation is simple to operate;
(4) The micro bacillus bacteria ZB21 strain obtained by screening can be preserved for a long time, can be continuously used after being preserved in an ultralow temperature refrigerator at minus 80 ℃ for one year, has no obvious reduction of the removal efficiency of the waste gas containing trimethylamine, and has better preservability.
Drawings
FIG. 1 is a schematic diagram of a bioreactor structure;
FIG. 2 is a colony morphology of the strain;
FIG. 3 is a phylogenetic tree diagram.
Detailed Description
The present invention will be further described in detail with reference to the following examples in order to make the objects, techniques and features of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1: isolated culture of Microbacterium ZB21
The biotrickling reactor involved in the isolation of the bacteria ZB21 of the genus Microbacterium is shown in FIG. 1.
The separation method comprises the following steps: 15g of acclimated bioreactor sludge is taken, diluted to three different concentration gradients of 5 times, 50 times and 500 times by double distilled water and respectively coated on a PDA bacterial solid culture medium. Each petri dish was coated with 2mL of diluted sludge solution, incubated at 32 ℃ for 3 days in an incubator, mycelia were picked from the edges of colonies, transferred to a fresh PDA plate for streaking, isolated culture, repeated until a pure culture was obtained, and transferred to PDA slant for preservation.
Example 2: morphological and molecular biological identification of Microbacterium ZB21
According to the handbook of bacteria identification Manual, the morphological characteristics of the micro bacillus bacteria ZB21 are observed under a microscope, specifically, the selected bacterial strain is inoculated on a PDA flat plate by adopting a dibbling method, and is cultured at the constant temperature of 32 ℃, and the morphological characteristics of the bacterial strain including the characteristics of colony morphology, color, size, edge characteristics, hypha character, growth speed and the like are observed with naked eyes.
The morphological characteristics of the microbacterium ZB21 of the present invention are as follows:
as shown in FIG. 2, the strain ZB21 of the genus Microbacterium is a gram-positive bacillus on a PDA culture medium, and the colony is round, has a diameter of 1.8-3.6mm, is yellowish white and has a smooth surface.
Example 3: molecular biological identification of Microbacterium ZB21
1. Genomic DNA extraction
The genomic DNA extraction method is as follows: (1) Selecting 2mL of bacterial liquid, centrifuging at 4deg.C and 12000rpm for 3 min, and collecting bacterial cellsThe method comprises the steps of carrying out a first treatment on the surface of the (2) Adding 600 μl of 2×CTAB (containing 2% beta-mercaptoethanol), quick freezing in liquid nitrogen for 1 min, transferring to water bath at 64deg.C for 1 min, repeating the above process for 3 times, and shaking at high speed for 2min, and water bath at 64deg.C for 30 min; (3) adding an equal volume of chloroform: isoamyl alcohol (volume ratio of 24:1), mixing the mixture upside down, standing on ice for 3 minutes, centrifuging at 4 ℃ and 12000rpm for 15 minutes, and taking the supernatant to a new centrifuge tube; (4) Adding equal volume of isopropanol into the supernatant, mixing gently upside down, standing on ice for 30 min, and centrifuging at 4deg.C and 12000rpm for 5 min; (5) The supernatant was discarded, the precipitate was washed with 75% absolute ethanol, and then, bacterial DNA was obtained by air-drying at room temperature, followed by ddH 2 O dissolves the DNA precipitate and the precipitate is stored in a refrigerator for later use.
2. Microbacterium ZB21 ITS-PCR amplification and molecular characterization
The Internal Transcribed Spacer (ITS) of the rRNA gene of the bacterial genome was amplified by PCR using bacterial universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3', SEQ ID NO. 2) and 1492R (5'-TACGGCTACCTTGTTACGACTT-3', SEQ ID NO. 3).
PCR reaction System (20. Mu.L): 2X Taq PCR MasterMix. Mu.L, 1. Mu.L of the upstream primer (10. Mu. Mol/L), 1. Mu.L of the downstream primer (10. Mu. Mol/L), 1. Mu.L of the DNA template (50 ng/L), and ddH were used 2 O was made up to a volume of 20. Mu.L.
Amplification conditions for PCR amplification on a PCR apparatus: pre-denaturation at 94 ℃ for 5 min; 32 cycles: denaturation at 94℃for 50 seconds, annealing at 58℃for 50 seconds, extension at 72℃for 1 minute for 30 seconds; further extension was carried out at 72℃for 10 minutes. Finally, purifying the PCR product and then carrying out sample feeding and sequencing; the nucleotide sequence obtained by sequencing was subjected to BLAST alignment in NCBI, and the alignment showed that the homology with Microbacterium (GenBank accession No. MH 845736) was 99.86%, so that the strain was identified as Microbacterium. The 16S rDNA sequence of the Microbacterium (Exiguobacterium sp.) bacterium ZB21 is as follows, SEQ ID NO.1.
Example 4: phylogenetic analysis of Microbacterium bacteria ZB21
BLAST search of the obtained sequence of ZB21 of the genus Microbacterium in NCBI was performed, and the known ITS sequences were downloaded in the vicinity thereof for constructing phylogenetic tree. Multiple sequence alignment was performed using clustalx1.83, the alignment was performed using MEGA7.0 software and using Neighbor training statistical methods, bootstrap values were set to 1000, and a Tamura-Nei model base substitution pattern was used to construct phylogenetic trees based on rDNA ITS sequences. A specific phylogenetic tree is shown in fig. 3.
Example 5: fermentation of microbacterium ZB21 and preparation of filler
After the culture and purification by PDA solid culture medium, the micro bacillus bacteria ZB21 are inoculated to the fermentation culture medium, and the amplification culture is carried out according to the volume ratio of the seed solution to the fermentation culture medium of 1:9. The fermentation medium is yeast powder 10.0g/L, peptone 10.0g/L, naCl 8.0.0 g/L and agar 15.0g/L. The fermentation culture temperature is 32-37 ℃, the dissolved oxygen is more than 1.8mg/L, the pressure is 0.08MPa, and the culture time is 60 hours. And (3) placing the fermentation liquor in a refrigerated centrifuge, centrifuging at 4 ℃ and 4000rpm for 30 minutes, removing supernatant, collecting precipitated thalli, and re-suspending the thalli by using fresh sterile culture solution to obtain bacterial suspension with the final concentration of 0.8-1.0 g/L. The ZB21 suspension is adsorbed on the filler to complete the preparation of the filler rich in ZB21 bacteria.
Example 6: microbacterium bacteria ZB21 improves absorption and removal efficiency of biological trickling filtration reactor on trimethylamine-containing waste gas
Two sets of parallel biological trickling filtration tower reactors (a control group and a treatment group) are designed, an original filler matrix without ZB21 bacteria and activated sludge inoculated by artificially adding bacteria obtained in the embodiment are respectively mixed in the filler matrix, and the difference of the absorption and removal efficiencies of the two sets of equipment to the trimethylamine-containing waste gas in one working day period is detected. After two sets of equipment are selected and started for 7 days, sampling is performed to determine the required parameters. The concentration of trimethylamine contained in the waste gas at the input port of the reactor of the biotrickling filter is 200mg/m respectively 3 、400mg/m 3 、600mg/m 3 、800mg/m 3 And 1000mg/m 3 And (5) measuring the degradation rate of trimethylamine. The measurement results are shown in Table 1. As can be seen from the data results in table 1, the removal efficiency of the treatment group biotrickling filter reactor for trimethylamine-containing waste gas is between 99.3% and 99.9%, which is significantly higher than that of the control group.
Example 7: shortening the start-up time of a biotrickling filter reactor by using a bacterium ZB21 of the genus Microbacterium
Two parallel biological trickling filtration tower reactors of a control group and a treatment group are designed, an original filler matrix without ZB21 bacteria and activated sludge inoculated by artificially adding the bacteria obtained in the embodiment are respectively mixed in the filler matrix, and the trimethylamine-containing waste gas removal efficiency from the first day to the 8 th day after the start is detected. The sampling time is fixed at 8 am every day, the waste gas samples of the input port and the output port are extracted, and the trimethylbenzene gas content is measured in mg/m 3 . The measured data are shown in Table 2.
The results in Table 2 show that the biotrickling filter reactors of the treatment group have reached an efficiency of 95.4% from day 4, and 99.9% by day 7; whereas the control biotrickling filter reactor reached 87.6% efficiency from day 6 and then gradually became stable. The data statistics of Table 2 show that the start-up time of the biotrickling filter reactor can be greatly shortened by the Microbacterium ZB21 bacteria.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the particular embodiments disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.
Sequence listing
<120> Microbacterium ZB21 and application thereof in trimethylamine waste gas degradation
<160> 3
<170> SIPOSequenceListing 1.0
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<211> 1424
<212> DNA
<213> fungus ZB21 (Exiguobacterium)
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atgcagtcga gcgcaggagc cgtctgaacc cttcgggggg acgacggtgg aatgagcggc 60
ggacgggtga gtaacacgta aagaacctgc ccataggtct gggataacca cgagaaatcg 120
gggctaatac cggatgtgtc atcggaccgc atggtccgct gatgaaaggc gctccggcgt 180
cgcccatgga tggctttgcg gtgcattagc tagttggtgg ggtaacggcc caccaaggcg 240
acgatgcata gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga 300
ctcctacggg aggcagcagt agggaatctt ccacaatgga cgaaagtctg atggagcaac 360
gccgcgtgaa cgatgaaggc tttcgggtcg taaagttctg ttgtaaggga agaacaagtg 420
ccgcaggcaa tggcggcacc ttgacggtac cttgcgagaa agccacggct aactacgtgc 480
cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg aattattggg cgtaaagcgc 540
gcgcaggcgg cctcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggccattg 600
gaaactggga ggcttgagta taggagagaa gagtggaatt ccacgtgtag cggtgaaatg 660
cgtagagatg tggaggaaca ccagtggcga aggcgactct ttggcctata actgacgctg 720
aggcgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 780
atgagtgcta ggtgttggag ggtttccgcc cttcagtgct gaagctaacg cattaagcac 840
tccgcctggg gagtacggtc gcaaggctga aactcaaagg aattgacggg gacccgcaca 900
agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccaa ctcttgacat 960
ccccctgacc ggtacagaga tgtatcttcc ccttcggggg caggggtgac aggtggtgca 1020
tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080
tgtccttagt tgccagcatt tggttgggca ctctagggag actgccggtg acaaaccgga 1140
ggaaggtggg gatgacgtca aatcatcatg ccccttatga gttgggctac acacgtgcta 1200
caatggacgg tacaaagggc agcgaagccg cgaggtggag ccaatcccag aaagccgttc 1260
tcagttcgga ttgcaggctg caactcgcct gcatgaagtc ggaatcgcta gtaatcgcag 1320
gtcagcatac tgcggtgaat acgttcccgg gtcttgtaca caccgcccgt cacaccacga 1380
gagtttgcaa cacccgaagt cggtgaggta accgtaagag ccag 1424
<210> 2
<211> 20
<212> DNA
<213> Artificial sequence (Unknown)
<400> 2
agagtttgat cctggctcag 20
<210> 3
<211> 22
<212> DNA
<213> Artificial sequence (Unknown)
<400> 3
tacggctacc ttgttacgac tt 22
Claims (9)
1. A bacterial strain ZB21 is characterized by being classified as a bacterium of the genus Microbacterium, and is classified asExiguobacterium sp.zb21, deposited in chinese collection of typical cultures, address: china, university of Wuhan, 430072, accession number: CCTCC No. M2021370, date of preservation: 2021, 4, 21;
the biological characteristics are as follows: the strain is gram-positive bacillus, and the colony is round, has a diameter of 1.8-3.6-mm, is yellow-white and has a smooth surface.
2. A bacterial strain ZB21 according to claim 1, characterized in that ITS rDNA ITS sequence is set forth in SEQ ID NO: 1.
3. Use of a bacterial strain in the degradation of trimethylamine-containing waste gas, characterized in that the bacterial strain employs one bacterial strain ZB21 according to claim 1.
4. Use according to claim 3, characterized in that the trimethylamine-containing waste gas is purified by degradation in a biotrickling filter reactor.
5. The method according to claim 4, wherein the filler in the biotrickling filter reactor comprises a matrix and bacterial ZB21, wherein the matrix per gram of the filler contains not less than 4.0X10-g 7 Bacterial ZB21 of CFU.
6. The use according to claim 5, wherein the matrix is a mixture of activated carbon, ceramsite and wood chips, and the mass ratio is 1:1:2.
7. use according to any one of claims 3 to 6, characterized in that the operating parameters of the biotrickling filter reactor are: tower height 8000mm, tower diameter 1500mm, packing height 1200mm, exhaust gas flow 3000m 3 /h; spray density of 12m 3 /（m 2 H) residence time of 10s.
8. A fermented product characterized by being a liquid inoculant of bacterial strain ZB21 according to claim 1.
9. A fermented product according to claim 8, characterized in that the preparation method of the liquid inoculant of a bacterial strain ZB21 is as follows:
placing the fermentation liquor of a bacterial strain ZB21 in a ultralow temperature centrifugal machine at 4 ℃, centrifuging at 4000rpm for 15min, removing supernatant, collecting precipitated thalli, and re-suspending thalli by fresh sterile culture liquor to obtain a liquid microbial inoculum with the final bacterial concentration of 0.5-0.8 g/L; wherein the temperature of fermentation culture is 32-37 ℃, the dissolved oxygen is more than 1.8mg/L, the pressure is 0.08MPa, and the culture time is 60 h;
the fermentation culture medium is yeast powder 10.0g/L, peptone 10.0g/L, naCl 8.0.0 g/L and agar 15.0g/L.
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| JP2018033437A (en) * | 2016-09-01 | 2018-03-08 | 毅 小田 | Microbe and enzyme which degrade trimethylamine and method of application |
| CN109055254A (en) * | 2018-06-27 | 2018-12-21 | 广东工业大学 | One plant of Exiguobacterium sp and its application with monomethyl amine degradation capability |
| CN110819537A (en) * | 2019-09-17 | 2020-02-21 | 杭州师范大学 | Microdiplodia fungus HNU107 and application thereof in ammonia waste gas degradation |
| CN111454865A (en) * | 2020-04-16 | 2020-07-28 | 江苏兆鋆新材料股份有限公司 | Microbacterium and application thereof |
| WO2021093290A1 (en) * | 2019-11-12 | 2021-05-20 | 青岛科技大学 | Protease-producing deep-sea exiguobacterium mutant strain and use thereof |
Family Cites Families (1)
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| WO2015100431A2 (en) * | 2013-12-24 | 2015-07-02 | Symbiota, Inc. | Plants containing beneficial endophytes |
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| CN109055254A (en) * | 2018-06-27 | 2018-12-21 | 广东工业大学 | One plant of Exiguobacterium sp and its application with monomethyl amine degradation capability |
| CN110819537A (en) * | 2019-09-17 | 2020-02-21 | 杭州师范大学 | Microdiplodia fungus HNU107 and application thereof in ammonia waste gas degradation |
| WO2021093290A1 (en) * | 2019-11-12 | 2021-05-20 | 青岛科技大学 | Protease-producing deep-sea exiguobacterium mutant strain and use thereof |
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