CN115067362A - Sterilization composition containing rhodopseudomonas palustris and tricyclazole - Google Patents
Sterilization composition containing rhodopseudomonas palustris and tricyclazole Download PDFInfo
- Publication number
- CN115067362A CN115067362A CN202210972109.6A CN202210972109A CN115067362A CN 115067362 A CN115067362 A CN 115067362A CN 202210972109 A CN202210972109 A CN 202210972109A CN 115067362 A CN115067362 A CN 115067362A
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- China
- Prior art keywords
- rhodopseudomonas palustris
- tricyclazole
- rice
- rice blast
- bacterial liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a sterilization composition containing rhodopseudomonas palustris and tricyclazole, wherein the mass ratio of rhodopseudomonas palustris bacterial liquid to tricyclazole is 1:20-20: 1; the concentration of rhodopseudomonas palustris bacterial liquid is 1-9 multiplied by 10 9 cfu/mL. The bactericidal composition can be used for preventing and treating rice blast, has a remarkable synergistic effect, can effectively reduce the use dosage of active ingredients, and can slow down the generation of drug resistance of diseases.
Description
Technical Field
The invention belongs to the field of pesticide compositions, and particularly relates to a bactericidal composition containing rhodopseudomonas palustris and tricyclazole and application thereof.
Background
China is a big agricultural country, and rice is an important grain source in China, so that the quality and the disease control of rice are always important matters concerned by people. The rice blast is one of the main diseases of rice, and the rice can be attacked in the whole growth period. The disease can occur in leaves, nodes, ears and necks, grains and other parts, and the common diseases include leaf blast, ear blast, stalk blast and the like. The rice blast is common in China, can cause withering of rice seedling leaves and short joints when the rice blast occurs seriously, forms white ears and blighted grains, and has great influence on the yield of rice when the rice blast occurs seriously. The prevention and control of the rice blast mainly adopts the measures of breeding disease-resistant varieties, preventing and controlling medicaments, strengthening cultivation management and the like. Most of the disease-resistant varieties are vertical resistant varieties based on the theory of gene-to-gene, the genetic variation of rice blast germs is very frequent, and the physiological race composition and structure of a single disease-resistant variety are easy to change after long-term large-area use, so that the disease resistance is lost.
Chemical control has the advantages of economy, effectiveness, convenience, quick response and the like, so that the chemical control is always an important component in a comprehensive rice blast treatment system. However, the pesticide resistance is enhanced and found out untimely, the pesticide application is extremely unscientific and reasonable, the pesticide application dosage and times seriously exceed the standard, and serious problems of environmental pollution, agricultural product quality safety and the like are caused. Therefore, how to reduce the pesticide consumption and comprehensively prevent and control rice blast in a green way is still an important problem in rice production in China at present.
The applicant finds that rhodopseudomonas palustris has excellent control effect on rice blast and false smut in earlier researches. For example, CN201610333926 discloses the use of Rhodopseudomonas palustris fermentation broth for controlling rice blast, wherein said Rhodopseudomonas palustris fermentation broth is obtained by culturing Rhodopseudomonas palustris LY-6(Rhodopseudomonas palustris LY-6). CN201610333888 discloses application of Rhodopseudomonas palustris biocontrol agent in preventing and controlling rice blast, which is characterized in that the Rhodopseudomonas palustris biocontrol agent is obtained by culturing Rhodopseudomonas palustris LY-6(Rhodopseudomonas palustris LY-6). In addition, the applicant also researches that the rhodopseudomonas palustris has good control effect on various diseases such as potato scab, tomato chlorosis virus, potato late blight, bacterial streak and the like.
The tricyclazole is a common rice bactericide in China at present, belongs to a systemic bactericide, can be quickly absorbed by each part of rice after application, has long lasting period, stable pesticide effect and low dosage, and can be used for preventing and treating rice blast. However, long-term use of tricyclazole results in serious drug resistance of diseases.
In the prior art, no report is provided about the application of the combination of rhodopseudomonas palustris and tricyclazole in rice blast control.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the following technical scheme:
a sterilization composition containing rhodopseudomonas palustris and tricyclazole is disclosed, wherein the mass ratio of rhodopseudomonas palustris bacterial liquid to tricyclazole is 1:20-20: 1; the concentration of rhodopseudomonas palustris bacterial liquid is 1-9 multiplied by 10 9 cfu/mL. Preferably, the concentration of the rhodopseudomonas palustris bacterial liquid is 2-5 multiplied by 10 9 cfu/mL。
The bactericidal composition also comprises auxiliary material components acceptable for pesticides, and the prepared pesticide is any acceptable preparation. Such as suspending agents, wettable powders and the like
The bactericidal composition provided by the invention is used for preventing and treating rice blast.
Preferably, Rhodopseudomonas palustris LY-6(Rhodopseudomonas palustris LY-6) adopted in the application is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2014525, the preservation unit address is located at Wuhan university, China, the preservation date is 2014, 10 and 29, and the strain is named as Rhodopseudomonas palustris LY-6(Rhodopseudomonas palustris LY-6) (the Rhodopseudomonas palustris LY-6 has applied for patent protection, and the patent application number is 201410750073.2).
Reference is made to the preparation of Rhodopseudomonas palustris LY-6 (patent application No.: 201410750073.2):
(1) and (3) activation: culturing the preserved strain of the rhodopseudomonas palustris strain by a double-layer plate culture method until a red single colony appears;
(2) seed culture: inoculating the red single colony cultured in the step (1) into a serum bottle, and culturing the red single colony in a photosynthetic bacteria liquid culture medium to a logarithmic phase under the conditions of the temperature of 30-32 ℃, the illumination condition of 2500 Lx-4000 Lx and the pH value of 7.0 to obtain a bacterial liquid;
(3) production and culture: inoculating the bacterial liquid obtained after the seed culture in the step (2) into a production bottle, and performing production culture by using a photosynthetic bacteria production culture medium, wherein the inoculum size of the bacterial liquid is 5 percent of the total volume of the photosynthetic bacteria strain production culture medium, and the bacterial liquid is cultured to a logarithmic phase under the conditions that the temperature is 30-32 ℃, the illumination condition is 2500 Lx-4000 Lx and the pH = 7.0;
concentration of rhodopseudomonas palustris bacterial liquid is 1-9 x 10 9 cfu/mL。
By adopting the technical scheme, the invention has the following beneficial effects:
the rhodopseudomonas palustris and tricyclazole are compounded to have obvious synergistic effect on rice blast germs, the use amount of active ingredients can be reduced, and the generation of drug resistance of diseases is slowed down.
Detailed Description
The present invention will be further explained with reference to specific embodiments.
Example 1: indoor toxicity determination of compound agent on rice blast
Test strains: pyricularia oryzae (Magnaporthe oryzae); and (4) collecting, separating and purifying in an experiment base.
Reagent to be tested:
rhodopseudomonas palustris bacterial liquid (a): concentration 5X 10 9 cfu/mL；
Tricyclazole (B), technical grade, is commercially available.
The test method comprises the following steps:
inhibition by hypha growth rate method. On the basis of a pre-experiment, each test medicament is prepared into dilution with a series of concentrations under the aseptic condition. Adding the mixture into a melted PDA culture medium according to a certain proportion under an aseptic condition, mixing, pouring into a sterilized culture dish, and preparing a series of drug-containing culture medium flat plates with concentration gradients; the rice blast strain to be tested is firstly cultured on a PDA culture medium at 25 ℃ for 5-7 days, when bacterial colonies grow to the edge of a culture dish, a punch with the diameter of 5mm is used for punching bacterial cakes at the edge of the bacterial colonies, and then the bacterial cakes are moved to the center of a culture medium plate containing the medicine. Control was performed on non-dosed plate medium. 1 block per plate, 1 treatment per dose, 4 replicates per treatment.
According to the growth condition of the bacteria in a blank control culture dish, investigating the growth condition of hyphae of pathogenic bacteria, measuring the diameter of a bacterial colony by using a caliper, measuring each bacterial colony for 2 times by a cross method, representing the size of the bacterial colony by the average number of the bacterial colony, and calculating the bacteriostasis rate and the virulence index, wherein the calculation formula is as follows: the inhibition rate is (control colony diameter-treatment colony diameter)/control colony diameter × 100%.
Calculating the virulence index and the cotoxicity coefficient (CTC) of the medicament according to the Sun Yunpei method.
Measured virulence index (ATI) ═ (standard agent EC 50/test agent EC50) × 100;
theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ];
when CTC is less than or equal to 80, the composition shows antagonism; when 80 < CTC < 120, the composition exhibits additive effects; when the CTC is more than or equal to 120, the composition shows synergistic effect.
And (3) testing results: the rhodopseudomonas palustris and tricyclazole are compounded to have obvious synergistic effect on rice blast germs, the use amount of active ingredients can be reduced, and the generation of drug resistance of diseases is slowed down.
Table 1: indoor toxicity determination of compound reagent on rice blast
Example 2 field control of Rice blast with Compound Agents
Test site: chunhua test base
And (3) testing crop diseases: blast of rice
The test method comprises the following steps: drug treatments and blank controls were set, each treatment was repeated 3 times, the cell area was 50m2, the cells were guard rows and randomly block-arranged. And in the rice cropping period, spraying clean water with the same amount on the leaf surfaces and the ear parts of the rice in a blank control area by adopting a manual sprayer. And no other bactericide is sprayed during the test period, and the field management is carried out according to the convention.
30 days after the pesticide is applied, the disease condition of the rice panicle plague is investigated by a parallel skip sampling method, 20 holes are investigated in each cell, and the disease index and the control effect are calculated (the disease index refers to GBT17980.19-2000, the field efficacy test criterion-the bactericide controls the rice leaf diseases).
And (3) testing results: compared with the single use, the compound of the rhodopseudomonas palustris and the tricyclazole can obviously improve the control effect of the pesticide on the rice blast.
Table 2: field control effect of compound agent on rice blast
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same.
Claims (6)
1. A sterilization composition containing rhodopseudomonas palustris and tricyclazole is characterized in that the mass ratio of rhodopseudomonas palustris bacterial liquid to tricyclazole is 1:20-20: 1; the concentration of rhodopseudomonas palustris bacterial liquid is 1-9 multiplied by 10 9 cfu/mL。
2. The bactericidal composition of claim 1, wherein the Rhodopseudomonas palustris is Rhodopseudomonas palustris LY-6(Rhodopseudomonas palustris LY-6) deposited in the china type culture collection with the collection number of CCTCC NO: m2014525.
3. The bactericidal composition of claim 2, wherein the concentration of rhodopseudomonas palustris is 2-5 x 10 9 cfu/mL。
4. The bactericidal composition of any one of claims 1-3, further comprising a pesticidally acceptable adjuvant ingredient.
5. The bactericidal composition of claim 4, wherein the bactericidal composition is formulated as a suspension concentrate or a wettable powder.
6. Use of the fungicidal composition according to claims 1 to 5 for the control of rice blast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210972109.6A CN115067362B (en) | 2022-08-15 | 2022-08-15 | Sterilization composition containing rhodopseudomonas palustris and tricyclazole |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210972109.6A CN115067362B (en) | 2022-08-15 | 2022-08-15 | Sterilization composition containing rhodopseudomonas palustris and tricyclazole |
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| CN115067362A true CN115067362A (en) | 2022-09-20 |
| CN115067362B CN115067362B (en) | 2023-08-11 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135285A (en) * | 2016-05-19 | 2016-11-23 | 湖南省植物保护研究所 | The application in prevention and control rice blast of the Rhodopseudomonas palustris biocontrol agent |
| KR20170133883A (en) * | 2016-05-27 | 2017-12-06 | 제주특별자치도(농업기술원) | Novel photosynthetic bacteria and method for promoting the growth of plants using it |
| CN110862278A (en) * | 2019-12-06 | 2020-03-06 | 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) | Facultative anaerobe biological fertilizer |
| CN112481162A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial compound inoculant and application thereof |
-
2022
- 2022-08-15 CN CN202210972109.6A patent/CN115067362B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135285A (en) * | 2016-05-19 | 2016-11-23 | 湖南省植物保护研究所 | The application in prevention and control rice blast of the Rhodopseudomonas palustris biocontrol agent |
| KR20170133883A (en) * | 2016-05-27 | 2017-12-06 | 제주특별자치도(농업기술원) | Novel photosynthetic bacteria and method for promoting the growth of plants using it |
| CN110862278A (en) * | 2019-12-06 | 2020-03-06 | 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) | Facultative anaerobe biological fertilizer |
| CN112481162A (en) * | 2020-11-27 | 2021-03-12 | 广东丽豪生物农业有限公司 | Microbial compound inoculant and application thereof |
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| CN115067362B (en) | 2023-08-11 |
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