CN115067349A - Weeding composition containing nicosulfuron and ametryn and application thereof - Google Patents
Weeding composition containing nicosulfuron and ametryn and application thereof Download PDFInfo
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- CN115067349A CN115067349A CN202110263345.6A CN202110263345A CN115067349A CN 115067349 A CN115067349 A CN 115067349A CN 202110263345 A CN202110263345 A CN 202110263345A CN 115067349 A CN115067349 A CN 115067349A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/66—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms
- A01N43/68—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms with two or three nitrogen atoms directly attached to ring carbon atoms
- A01N43/70—Diamino—1,3,5—triazines with only one oxygen, sulfur or halogen atom or only one cyano, thiocyano (—SCN), cyanato (—OCN) or azido (—N3) group directly attached to a ring carbon atom
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention belongs to the field of pesticides, and particularly relates to a weeding composition containing nicosulfuron and ametryn and application thereof. The weeding composition comprises an active ingredient A and an active ingredient B with effective weeding amount, wherein the active ingredient A is nicosulfuron; the active component B is ametryn. The composition can effectively prevent and kill various weeds such as gramineous weeds and broadleaf weeds, and has the characteristics of expanding a weed control spectrum, reducing application amount, generating a synergistic effect, solving resistant weeds and the like.
Description
Technical Field
The invention belongs to the field of pesticides, and particularly relates to a herbicidal composition containing nicosulfuron and ametryn and application thereof.
Background
Chemical weeding is the most economic and effective means in preventing and removing weeds in farmlands, but the problems of drug resistance and resistance evolution of weeds and the like are easily caused by using a single variety or a single action mode of chemical herbicide continuously at a high dose for a long time.
Nicosulfuron belongs to a selective herbicide of sulfonylureas, is a systemic herbicide, can be absorbed by stems, leaves and roots of weeds, and then is conducted in plants to cause growth arrest of sensitive plants, chlorosis of stems and leaves, and gradual withering.
Ametryn is a triazine selective systemic conduction herbicide. It has inhibiting effect on plant photosynthesis and is one kind of selective herbicide.
The reasonable compounding or mixing of the herbicide compound has the advantages of expanding the weed spectrum, improving the control effect, delaying the occurrence and development of drug resistance and drug resistance of weeds and the like, and is one of the most effective methods for solving the problems. Therefore, the development of a weeding composition variety which has high safety and wide weed control spectrum, can generate a synergistic effect and solve the problem of resistant weeds is urgently needed in production.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a weeding composition containing nicosulfuron and ametryn and application thereof, wherein the composition can effectively prevent and kill various weeds, especially grassy weeds and broadleaf weeds, and has the characteristics of expanding a weeding spectrum, reducing application amount, generating a synergistic effect, solving resistant weeds and the like.
A herbicidal composition comprising nicosulfuron and ametryn, characterized by comprising a herbicidally effective amount of an active ingredient A and an active ingredient B, wherein,
the active component A is nicosulfuron (CAS number: 111991-09-4);
the active component B is ametryn (CAS number: 834-12-8);
wherein the weight ratio of A to B is 1-100: 1-100, 1-80: 1-80, 1-50: 1-50, 1-30: 1-30, 1-20: 1-25 or 1-5: 1-20.
The herbicidal composition further includes an active ingredient C selected from one of quinclorac (CAS number: 84087-01-4), picloram (CAS number: 1918-02-1), aminopyralid (CAS number: 150114-71-9), quinmerac (CAS number: 90717-03-6), compound C' (CAS number: 2445983-82-2), clopyralid (CAS number: 1702-17-6), halauxifen (CAS number: 943831-98-9), clethodim (CAS number: 99129-21-2), quizalofop-p (CAS number: 100646-51-3), and kokusne (CAS number: 72619-32-0).
Wherein the weight ratio of A, B to C is 1-100: 0.1-100, 1-80: 0.2-80, 1-50: 0.5-60, 1-30: 1-50, 1-20: 1-25: 2-20 or 1-5: 1-20: 5-10.
The mass percentage of the active ingredients in the weeding composition accounts for 1-95% of the total amount, and preferably 10-80%.
The weeding composition also comprises conventional auxiliaries, wherein the conventional auxiliaries comprise a carrier and/or a surfactant.
The term "carrier" as used herein means an organic or inorganic, natural or synthetic substance. They facilitate the application of the active ingredient, the carrier being generally inert and must be agriculturally acceptable, in particular for the treated plant. The carrier may be a solid, such as a clay, natural or synthetic silicate, silica, resin, wax, solid fertilizer, or the like; or liquids such as water, alcohols, ketones, petroleum distillates, aromatic or waxy hydrocarbons, chlorinated hydrocarbons, liquefied gases, etc.
Surfactants may include emulsifying, dispersing or wetting agents, which may be ionic or non-ionic. Examples which may be mentioned are salts of polyacrylic acids, lignosulphonates, salts of phenolsulphonic or naphthalenesulphonic acids, polymers of ethylene oxide with aliphatic alcohols or with aliphatic acids or with aliphatic amines and substituted phenols, in particular alkylphenols or arylphenols, sulphosuccinates, taurine derivatives, in particular taurine alkyl esters, and phosphoric esters of alcohols or polyhydroxyethylated phenols, alkylsulfonates, alkylarylsulphonates, alkylsulfates, lauryl ether sulphates, fatty alcohol sulphates, and sulphated hexadec, heptadeca-and octadecanols and sulphated fatty alcohol glycol ethers, and furthermore condensates of naphthalene or naphthalenesulphonic acids with phenol and formaldehyde, polyoxyethylene octylphenyl ether, ethoxylated isooctylphenol, octylphenol or nonylphenol, alkylphenyl polyglycol ethers, tributylphenyl polyglycol ether, tristearylphenyl polyglycol ether, polystearylphenyl polyglycol ether, Alkylaryl polyether alcohols, alcohol and fatty alcohol/ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ether acetal, sorbitol esters, lignosulfite waste liquors, and also proteins, denatured proteins, polysaccharides (e.g. methylcellulose), hydrophobically modified starches, polyvinyl alcohols, polycarboxylates, polyalkoxylates, polyvinylamines, polyvinylpyrrolidone and copolymers thereof. At least one surfactant is required to be present to facilitate dispersion of the active ingredients in water and to facilitate their proper application to the plant.
The compositions may also contain various other components such as protective colloids, binders, thickeners, thixotropic agents, penetrants, stabilizers, chelating agents, dyes, colorants, and polymers.
The herbicidal composition further comprises at least one safener, preferably one or more of isoxadifen (CAS: 163520-33-0), cyprosulfamide (CAS: 221667-31-8), mefenpyr (CAS: 135590-91-9), cloquintocet (CAS: 99607-70-2), gibberellic acid (CAS: 7-06-5), furilazole (CAS: 121776-33-8), metacamifen (CAS: 129531-12-0).
In the context of the present specification, if the abbreviated forms of the common name of the active compounds are used, all customary derivatives, such as esters and salts, and also isomers, in particular optical isomers, in particular one or more commercially available forms, are included in each case. If the generic name denotes esters or salts, all other customary derivatives, such as other esters and salts, free acids and neutral compounds, and also isomers, in particular optical isomers, in particular one or more commercially available forms, are also included in each case. The chemical name given for a compound means at least one compound covered by the generic name, with compounds generally being preferred. In the case of sulfonamides, such as sulfonylureas, salts also include those formed by exchange of cations with hydrogen atoms in the sulfonamide group.
In the context of the present invention, the salts of the compounds are preferably in the form of the respective alkali metal, alkaline earth metal or ammonium salts, preferably in the form of the respective alkali metal salts, more preferably in the form of the respective sodium or potassium salts, most preferably in the form of the respective sodium salts.
The compositions of the present invention may be diluted or used directly by the user prior to use. The formulation can be prepared by conventional processing methods, i.e., mixing the active substance with a liquid solvent or solid carrier, and adding one or more surfactants such as dispersants, stabilizers, wetting agents, binders, defoamers, etc.
The specific preparation of the weeding composition is dispersible oil suspending agent, water suspending agent, suspending emulsion, wettable powder, missible oil, water dispersible granule (dry suspending agent), emulsion in water and microemulsion.
In short, the compositions of the present invention may be mixed with solid and liquid additives conventionally used in prior art formulations. The amount of the effective ingredient to be used varies depending on external conditions such as temperature, humidity, the nature of the herbicide to be used, and the like. It may vary widely, for example between 0.001 and 1.0kg/ha, or more of active substance, but preferably between 0.005 and 750g/ha, in particular between 0.005 and 500 g/ha.
In addition, the compositions according to the invention can be applied by spraying to the foliage of the plants to be treated, i.e. to the weeds, in particular to the surfaces infested or liable to be infested with them.
Further, the invention also provides an application of the weeding composition in weed control; and to provide a method of controlling unwanted plant growth comprising applying the herbicidal composition to a plant, part of a plant, plant seed, or area where a plant is growing. Preferably, the herbicidal compositions are used to selectively control weeds in crops of useful plants, more preferably, the useful plants are transgenic plants or plants treated with genome editing techniques.
In one embodiment, the useful crop is a drug resistant oilseed rape which is tolerant/resistant to the herbicide contained in the composition, such as a known drug resistant or cross-resistant oilseed rape crop or a cultivated drug resistant or genetically modified oilseed rape crop.
Some tolerant oilseed rape crops obtained by recombinant techniques are known and have been put to practical use; reference is made to the article in the journal "beet" (Zuckerrube), volume 47(1998), page 217. Cross-resistance in transgenic plants can be found in WO-A-98/20144. In addition, ALS inhibitor-resistant plants obtained by selection methods or recombinant methods are disclosed in EP-A-0360750. Herbicide resistance in plants is obtained by increasing ALS content in plants. US-A-5,198,599 discloses sulfonylureA-and imidazolinone-tolerant plants obtained by A selection method.
Conventional methods of propagation of new plants with improved properties compared to existing plants include, for example, traditional breeding methods and mutant propagation. In other words, new plants with improved properties can be propagated by recombinant methods (see, for example, EP-A-221044, EP-A0131624). For example, several situations have been disclosed:
recombinant modification of crop genes with the aim of improving starch synthesis in plants (e.g.WO 92/11376, WO92/14827, WO91/19806),
transgenic crops which are resistant to other herbicides, for example to sulfonylurea herbicides (EP-A0257993, US-A5013659),
transgenic plants which produce Bacillus thuringiensis toxins (Bt toxins), i.e.which can be rendered resistant to certain pests (EP-A0142924, EP-A0193259),
transgenic crops with improved fatty acid composition (WO 91/13972).
In principle, many molecular biological techniques are known which can be applied for the propagation of new transgenic plants with improved properties; see, e.g., Sambrook et al, 1989, handbook of molecular cloning experiments, 2 nd edition, published by Cold Spring Harbor Laboratory, Cold Spring Harbor, NY; or Winnacker "Gene and clone", VCH Weinheim, 2 nd edition, 1996 or Christou, "trends in plant science" 1(1996) p.423-431.
For such recombination operations, the nucleic acid molecule may be introduced into a plasmid in which genetic mutation or sequence change may occur by a DNA sequence recombination method. For example, base substitutions, removal of portions of sequence or addition of natural or synthetic sequences may be made using standard methods as described above. The fragments are provided with linkers or linkers to interconnect the DNA fragments.
Plant cells having a reduced activity of a gene product can be prepared, for example, by expressing at least one corresponding antisense-RNA, sense-RNA to obtain a cosuppression effect, or by expressing at least one constructed ribozyme which is suitable for specifically cleaving the transcript of the above-mentioned gene product.
For this purpose, it is possible to use either DNA molecules which comprise the entire coding sequence of the gene product, including any flanking sequences present, or DNA molecules which comprise only parts of the coding sequence, but these parts must be sufficiently long to obtain an antisense effect in the cell. DNA sequences which are highly homologous to the coding sequence of the gene product but are not identical can also be used.
When the nucleic acid molecule is expressed in a plant, the synthesized protein can be localized to any desired region in the plant cell. However, to achieve localization in a particular region, for example, the coding region may be linked to a DNA sequence to ensure localization in the particular region. Such sequences are known to those skilled in the art (see, for example, Braun et al, EMBO J.11(1992, 3219-3227; Wolter et al, Proc. Natl. Acad. Sci. USA 85(1988), 846-850; Sonnewald et al, plant journal 1(1991), 95-106).
The transgenic plant cells can be regenerated into whole plants using known techniques. Transgenic plants can in principle be any desired plant species, i.e.both monocotyledonous and dicotyledonous plants.
In this way, transgenic plants with improved properties can be obtained by overexpression of homologous (═ native) genes or gene sequences, suppression or prevention or by expression of heterologous (═ foreign) genes or gene sequences.
When the herbicidal composition of the present invention is applied, an unexpected synergistic effect is obtained, and the herbicidal activity is more remarkable than the expected sum of the activities using individual herbicides, and the activities of the individual herbicides. The synergistic effect is manifested by reduced application rates, a broader spectrum of weed control, and a faster and longer lasting herbicidal action, which are desirable in the practice of weed control. These new compositions are clearly superior to existing herbicides in terms of the properties described, achieving reduced use and being more environmentally friendly.
The binary or ternary or higher weeding composition also has the following advantages:
(1) the composition of the invention is environment-friendly and is easy to degrade in the environment.
(2) The weeding composition has low cost and convenient use, and has great economic and social benefits when being popularized and applied.
Detailed Description
The following examples are not intended to limit the invention, but merely to illustrate how the invention may be carried out. These examples show particularly significant effectiveness against certain weeds. Examples are as follows:
A) examples of the invention
1. Nicosulfuron and ametryn (4+20) dispersible oil suspending agent
4% of nicosulfuron, 20% of ametryn, 5% of calcium dodecyl benzene sulfonate, 7% of fatty alcohol-polyoxyethylene ether, 4% of castor oil-polyoxyethylene ether, 3% of polyurethane dispersant, 0.6% of organobentonite, 0.6% of fumed silica, 6% of 100# solvent oil and methyl oleate, wherein the dispersible oil suspending agent processing equipment is as follows: mixing kettles, colloid mills, sand mills, shearing machines and the like.
The processing process of the dispersible oil suspending agent comprises the following steps: putting all materials into a mixing kettle, stirring and mixing, passing through a colloid mill, then entering a sand mill for three-stage sand milling, finally uniformly shearing in a shearing machine, and transferring to a storage tank for filling after the test is qualified.
B) Test of drug efficacy
1) Test targets: large Tang Dynasty.
2) Reagent: the raw medicines are all diluted by using an emulsifier Tween-80 aqueous solution with the content of 0.1 percent and taking acetone as a solvent.
3) The test method comprises the following steps:
spraying treatment of stems and leaves after seedling:
the method is characterized in that the weeds are cultured by a pot culture method, when the weeds are in a 4-5 leaf period, stem and leaf spray is adopted for uniform spraying, and the spraying amount is 450 kilograms per hectare.
4) Data investigation and statistical analysis
4.1) investigation method
The complete survival weed seedlings are cut off along the soil surface by a blade by adopting an absolute number survey method, and the fresh weight of the weeds is weighed by an analytical balance. For the weeds that have died, the fresh weight is zero.
4.2) investigation time and number of times
After the treatment, the test was conducted 21 days, and the total number of tests was 1.
4.3) statistical analysis of data
And (4) testing by a Colby method, and evaluating the combined action type of any two or three on the weeds.
And (3) shearing green tissues on each treatment ground at 21d, weighing by adopting an electronic balance, and calculating the actual survival rate: e (%) × 100 (fresh weight of weeds in treatment area/fresh weight of weeds in control area) × 100
The theoretical weed survival rate calculation formula is as follows: e0 (%) ═ X Y/100 (binary mixed use)
Wherein X represents the survival rate of weeds A at a dose of P, Y represents the survival rate of weeds B at a dose of Q, E0 represents the theoretical survival rate of weeds A + B at a dose of (P + Q), and E represents the actual survival rate of weeds for each treatment.
E0(%)=X×Y×Z×…×N/100 (n-1) (use of three or more elements)
X, Y, Z, N in the formula respectively represents the actual survival rates of n single agents, and n is the number of varieties of the compound herbicide.
When E0-E is more than 10%, the synergistic effect is generated; when E0-E < -10%, antagonism is indicated; when E0-E is within. + -. 10%, it is indicated that an additive effect is produced.
The statistical results of the combined action evaluation of each component to weeds are shown in tables 1-11 below.
Table 1A is nicosulfuron and B is ametryn
Table 2A is nicosulfuron, B is ametryn, C is quinclorac
Table 3A indicates nicosulfuron, B indicates ametryn, and C indicates picloram
Table 4A shows nicosulfuron, Topramezyn B and aminopyralid C
Table 5A shows nicosulfuron, B shows ametryn, and C shows quinmerac
TABLE 6A is nicosulfuron, B is ametryn, C is Compound C'
Table 7A is nicosulfuron, B is ametryn, C is clopyralid
Table 8A is nicosulfuron, B is ametryn, C is halauxifen
Table 9A shows nicosulfuron, Toramezone B and clethodim C
Table 10A shows nicosulfuron, Torasyn B and quizalofop-p-ethyl C
Table 11A for nicosulfuron, B for ametryn, and C for high-potency Gephycan
Note: e (A): observed fresh weight survival rate for component A treatment at a given dose
E (B): observed fresh weight survival for component B treatment at a given dose
E (C): observed fresh weight survival for component C treatment at a given dose
E (A + B): the observed fresh weight survival rate of the binary combination of component A, B at a given dosage
E0(a + B): theoretical fresh weight survival rate of binary blend of component A, B at given dose
E (A + B + C): the observed fresh weight survival rate of the three-component mixture of the component A, B, C at a given dosage
E0(a + B + C): theoretical fresh weight survival rate for ternary blend of component A, B, C at a given dose C) demonstration of the field
The herbicide composition prepared in example 1 was used for field weed effect test. Field crops: the ALS inhibitor and PSII inhibitor resistant rape mutants respectively generated by natural mutation or artificial mutagenesis polymerize the two gene mutations in an artificial sexual hybridization and backcross transfer mode to generate a nicosulfuron and ametryn herbicide resistance character polymerized double-resistant rape crop, and the generated weeds mainly comprise: bluegrass, pseudostellaria heterophylla, alopecurus, speedwell, vetch, caraway, etc.
The test method comprises the following steps: in the 3-4 leaf period of the weeds, a manual sprayer is used, and the water adding amount is 30 kg/667 m 2 Stem and leaf spray (F) is adopted.
The specific test agents and dosages are detailed in table 12. The control effect was examined 42 days after the application of the composition, as shown in Table 12.
Table 12 demonstration of field Effect of the compounded compositions
Through a large number of tests and explorations, the invention unexpectedly discovers that the composition has a surprising and unexpected synergistic effect when used for preventing and removing various weeds, particularly gramineous weeds and broadleaf weeds, the synergistic effect is more obvious under low dosage, the dosage can be reduced, the pollution to the environment is reduced, the agricultural cost is reduced by reasonable compounding, the composition is efficient to ALS and ACCase inhibitor resistant weeds, and the composition has a good application prospect. In addition, the introduction of tolerant or resistant rape crop varieties and lines, in particular transgenic rape crop varieties and lines, allows the addition of new active substances which are not themselves selective for common rape crop varieties to conventional weed control systems. Meanwhile, tests show that the herbicide composition has good selectivity and excellent synergistic effect in fields such as corn, rice, sugarcane, cotton and the like, and can be developed into herbicide mixtures with wide market value.
Claims (10)
1. A herbicidal composition comprising nicosulfuron and ametryn, characterized by comprising a herbicidally effective amount of an active ingredient A and an active ingredient B, wherein,
the active component A is nicosulfuron;
the active component B is ametryn.
2. A herbicidal composition according to claim 1, wherein the weight ratio of A to B is 1-100: 1-100, 1-80: 1-80, 1-50: 1-50, 1-30: 1-30, 1-20: 1-25 or 1-5: 1-20.
3. The herbicidal composition as claimed in claim 1, further comprising an active ingredient C selected from one of quinclorac, picloram, aminopyralid, quinmerac, a compound C' having CAS number 2445983-82-2, clopyralid, halauxifen, clethodim, quizalofop-p-ethyl and gabon.
4. A herbicidal composition according to claim 3, wherein the weight ratio of A, B to C is 1-100: 0.1-100, 1-80: 0.2-80, 1-50: 0.5-60, 1-30: 1-50, 1-20: 1-25: 2-20 or 1-5: 1-20: 5-10.
5. A herbicidal composition according to any one of claims 1 to 4, characterized in that the active ingredients in the herbicidal composition are present in an amount of 1 to 95% by weight, preferably 10 to 80% by weight, based on the total amount.
6. A herbicidal composition according to any of claims 1 to 4, characterized in that it further comprises conventional adjuvants, preferably comprising carriers and/or surfactants.
7. The herbicidal composition according to any one of claims 1 to 4, characterized in that it further comprises at least one safener, preferably the safener is selected from one or more of isoxadifen, cyprosulfamide, mefenpyr, cloquintocet, gibberellic acid, furilazole, metacamifen.
8. A herbicidal composition according to any one of claims 1 to 4, characterized in that the specific formulation of the herbicidal composition is dispersible oil-suspendable agent, water-suspendable agent, suspoemulsion, wettable powder, emulsifiable concentrate, water dispersible granules, aqueous emulsion or microemulsion.
9. Use of the herbicidal composition according to any one of claims 1 to 8 for controlling weeds; preferably, the herbicidal compositions are used for the selective control of weeds in crops of useful plants; more preferably, the useful crop is drug-resistant oilseed rape; further preferably, the useful crop is a transgenic crop or a crop treated with genome editing techniques.
10. A method of controlling unwanted plant growth, comprising applying the herbicidal composition of any one of claims 1 to 8 to a plant, part of a plant, plant seed, or area where a plant is growing; preferably, the herbicidal compositions are used to selectively control weeds in crops of useful plants; more preferably, the useful crop is drug-resistant oilseed rape; further preferably, the useful crop is a transgenic crop or a crop treated by genome editing techniques.
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CN108605967A (en) * | 2018-06-09 | 2018-10-02 | 合肥喜田生物科技有限公司 | A kind of mixed herbicide and its application containing nicosulfuron, atrazine and dichloro quinolinic acid |
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