CN115053945A - Method for reducing acrylamide content in small crispy meat - Google Patents

Method for reducing acrylamide content in small crispy meat Download PDF

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CN115053945A
CN115053945A CN202210495207.5A CN202210495207A CN115053945A CN 115053945 A CN115053945 A CN 115053945A CN 202210495207 A CN202210495207 A CN 202210495207A CN 115053945 A CN115053945 A CN 115053945A
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reducing
acrylamide
frying
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蔡克周
徐宝才
许朝阳
张晓敏
吴萌萌
韩飞
龙白雪
周辉
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Hefei University of Technology
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    • AHUMAN NECESSITIES
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    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/428Addition of flavours, spices, colours, amino acids or their salts, peptides, vitamins, yeast extract or autolysate, nucleic acid or derivatives, organic acidifying agents or their salts or acidogens, sweeteners, e.g. sugars or sugar alcohols; Addition of alcohol-containing products
    • AHUMAN NECESSITIES
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    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/70Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor
    • A23L13/72Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying
    • A23L5/11General methods of cooking foods, e.g. by roasting or frying using oil
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/41Retaining or modifying natural colour by use of additives, e.g. optical brighteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention relates to a method for reducing the content of acrylamide in crispy pork. The method comprises the following steps: preparing a curing agent and wrapping powder for food frying, wherein the curing agent comprises the following components in parts by weight: 1-2 parts of salt, 3-4 parts of cane sugar, 1-2 parts of monosodium glutamate, 3-5 parts of cooking wine and 1-3 parts of exogenous additives, wherein the wrapping powder for fried food comprises the following components: 60 parts of wheat flour, 40-80 parts of corn starch, 20-40 parts of resistant starch, 8 parts of soybean protein isolate, 4 parts of baking powder, 20 parts of soybean oil and 1 part of carrageenan; step two: mixing the processed pork fillet with the pickling agent, pickling for 30min, and coating with flour; step three: air frying the flour-coated pork fillet. The acrylamide content in the crispy meat was determined by HPLC. The method adopts an air frying mode to replace the traditional oil bath frying mode, has simple operation, greatly reduces the oil content of the food, not only reduces the content of acrylamide, but also effectively inhibits the generation of peculiar smells such as rancid smell and the like in the storage process, and the prepared crisp meat has golden color, is crispy and delicious, and is green and healthy.

Description

Method for reducing acrylamide content in small crispy meat
Technical Field
The invention belongs to the technical field of food safety production, and particularly relates to a method for reducing the content of acrylamide in crispy pork.
Background
Frying is a traditional processing mode, and fried food is popular with consumers because of being crisp and delicious and unique in flavor. However, with the concern of food safety, especially with the generation of harmful substances during food processing, there is increasing evidence that frying produces more harmful substances than cooking, grilling, and smoking. The traditional oil bath frying mode is usually accompanied by the generation of a large amount of oil smoke and a large amount of harmful substances, fine particulate matters and VOCs substances in the oil smoke cause great harm to human health and environment, and the problems of serious loss of high oil content and high nutrient content of fried food and the like are prominent.
An Air Fryer (AF) is a replacing process which is considered as traditional frying and is different from a traditional frying mode, the Air Fryer uses Air as a heat mass transfer carrier, Air is used for baking, and the Air Fryer has the advantages of being healthy, attractive, safe and easy to clean and the like, and is a novel frying technology which is expected to replace traditional frying.
Acrylamide (AA), one of the products of the maillard reaction, is a potentially carcinogenic substance with neurotoxicity, causing damage to peripheral nerves. Since the discovery of this carcinogen in fried foods was reported by the swedish national food administration and stockholm university in 2002, the public began to pay attention to the acrylamide content in foods, and many scholars and food companies began to focus on the research on methods for detecting and controlling acrylamide in foods.
There are mainly six aspects to the current methods for reducing or inhibiting acrylamide: (1) reducing or eliminating acrylamide-forming precursor species; (2) the processing temperature and the reaction time are reduced; (3) the pH value is reduced; (4) adding cysteine, etc.; (5) adding an exogenous active ingredient; (6) light radiation is applied.
The addition of exogenous active ingredients is the main way for researching the inhibition of the formation of acrylamide at present. The onion essential oil contains various active ingredients, not only can provide special flavor for the crisp meat, but also has the functions of reducing blood pressure, blood fat and cholesterol and purifying blood vessels. The edible acetic acid is a good acidity regulator and flavoring agent, and the content of the glacial acetic acid is more than 99.5%. Resistant starch (phosphate ester distarch, RS4) is a chemically modified starch that is difficult to absorb in the human small intestine and has a low glycemic index. The addition of resistant starch can effectively inhibit the generation of acrylamide and improve the quality characteristics of processed foods, such as better golden color, stronger water retention, increased brittleness and the like.
No influence on acrylamide formation after air frying has been reported. Acrylamide is commonly found in fried starch foods, and starch is added into the small crisp meat as one of raw materials in the manufacturing process, so that the content of the acrylamide cannot be ignored.
Disclosure of Invention
The invention aims to solve the problem of providing a method for reducing the acrylamide content in the small crisp meat, the small crisp meat fried by air has low acrylamide content and golden, fragrant, crisp and delicious color, and the unique flavor of the traditional oil bath frying processing mode is reduced to the maximum extent.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for reducing the acrylamide content in a small crisp meat comprises the following steps,
the method comprises the following steps: mixing the processed pork fillet with curing agent, and curing;
step two: wrapping the pickled pork fillet with wrapping powder for fried food;
step three: air frying the flour-coated pork fillet.
According to further optimization of the technical scheme, the curing agent comprises the following components in parts by weight: 1-2 parts of salt, 3-4 parts of cane sugar, 1-2 parts of monosodium glutamate, 3-5 parts of cooking wine and 1-3 parts of exogenous additives.
According to the technical scheme, the exogenous additives are onion essential oil and edible acetic acid, the edible acetic acid is diluted by 100 times and then added, and the mixing ratio is 1-2: 1.
According to the further optimization of the technical scheme, the treatment of the pork tenderloin comprises the step of removing visible fat and connective tissues on the surface of the pork tenderloin, and the specification of the treated pork tenderloin is 4cm multiplied by 1cm multiplied by 0.8 cm.
According to the further optimization of the technical scheme, the wrapping powder for the fried food comprises the following components in parts by weight: 60 parts of wheat flour, 40-80 parts of corn starch, 20-40 parts of resistant starch, 8 parts of soybean protein isolate, 4 parts of baking powder, 20 parts of soybean oil and 1 part of carrageenan.
According to the technical scheme, the proportion of the corn starch to the resistant starch in the coating powder for the fried food is further optimized to be 1-2: 1.
According to the technical scheme, the resistant starch is resistant starch RS 4.
The technical scheme is further optimized, the air frying temperature is 150-180 ℃, and the frying time is 15-18 min.
The technical scheme is further optimized, wherein the frying temperature is 160 ℃, and the frying time is 16 min.
According to the further optimization of the technical scheme, the pickling time is 30 min.
Different from the prior art, the technical scheme has the following advantages:
(1) the invention provides a method for reducing the acrylamide content in small crisp meat, which adopts onion essential oil and edible acetic acid as exogenous additive substances, not only endows the fried small crisp meat with special flavor, but also reduces the generation of acrylamide due to the fact that the onion essential oil contains various active ingredients. Meanwhile, the addition of edible acetic acid forms a slightly acidic environment, which is also one of the ways for inhibiting the formation of acrylamide.
(2) The invention adopts the air fryer as a processing mode and takes the air as a mass transfer carrier, so that the finished crisp meat has no difference with the traditional frying in taste, and the oil content of the food is effectively reduced.
(3) The invention adopts the added resistant starch to prepare the wrapping powder for the fried food, and has two effects on the quality of the small crisp meat: a) the resistant starch is generated by modifying common starch, the content of reducing sugar is reduced, so that precursors for generating acrylamide are reduced, and the resistant starch has stronger anti-gelatinization capability than the common starch and is beneficial to reducing the generation of the acrylamide. b) The resistant starch has better water retention and color protection capability, and can reduce the problems of more water loss and whitish color caused by longer air frying time to a certain extent.
(4) The various preparation and parameters of the invention are regarded as a whole and are mutually synergistic, so that the prepared crisp meat not only can effectively inhibit the generation of acrylamide, but also can well enhance the color, fragrance and taste of the crisp meat, and maximally restore the mouthfeel of the traditional frying way.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the technical means in detail, the following detailed description is given with reference to specific embodiments.
The first scheme is as follows:
a method for reducing the acrylamide content in the crisp meat comprises the following steps,
(1) pretreating raw meat: thawing frozen pork fillet in refrigerator at 4 deg.C for 24 hr, removing fat and connective tissue on the surface of pork fillet, and cutting into 4cm × 1cm × 0.8cm pieces.
(2) Preparing a curing agent, namely 1.2% of salt, 3.0% of cane sugar, 1.0% of monosodium glutamate and 3.0% of cooking wine, wherein the percentage of the curing agent is added according to the weight of the pork, and the processed pork fillet and the curing agent are fully stirred and uniformly mixed and cured for 30 min.
(3) Coating powder for frying the prepared food: 30% of wheat flour, 40% of corn starch, 4% of soybean protein isolate, 2% of baking powder, 10% of soybean oil and 0.5% of carrageenan (the mass ratio of powder to water is 1: 1).
(4) Frying in an oil bath pan: the frying temperature is 180 deg.C, and the frying time is 2.5 min.
Scheme two is as follows:
a method for reducing the acrylamide content in a small crisp meat comprises the following steps,
(1) pretreating raw meat: in the same scheme I
(2) Preparing a curing agent: 1.2% of salt, 3.0% of cane sugar, 1.0% of monosodium glutamate, 3.0% of cooking wine, 2.0% of onion essential oil and 0.1% of edible acetic acid. Mixing the processed pork fillet with the curing agent, stirring thoroughly, and curing for 30 min.
(3) Coating powder for frying the prepared food: 30% of wheat flour, 40% of corn starch, 4% of soybean protein isolate, 2% of baking powder, 10% of soybean oil and 0.5% of carrageenan (the mass ratio of powder to water is 1: 1).
(4) Frying in an oil bath: the frying temperature is 180 deg.C, and the frying time is 2.5 min.
The third scheme is as follows:
a method for reducing the acrylamide content in a small crisp meat comprises the following steps,
(1) pretreating raw meat: in the same scheme I
(2) Preparing a curing agent: 1.2% of salt, 3.0% of cane sugar, 1.0% of monosodium glutamate, 3.0% of cooking wine, 2.0% of onion essential oil and 0.1% of edible acetic acid, and the processed pork tenderloin and the curing agent are fully stirred and mixed uniformly, and cured for 30 min.
(3) Coating powder for frying the prepared food: 30% of wheat flour, 20% of corn starch, 20% of resistant starch, 4% of soybean protein isolate, 2% of baking powder, 10% of soybean oil and 0.5% of carrageenan (the mass ratio of powder to water is 1: 1).
(4) Frying in an oil bath: the frying temperature is 180 deg.C, and the frying time is 2.5 min.
The scheme four is as follows:
(1) pretreating raw meat: in the same scheme I
(2) Preparing a curing agent: 1.2% of salt, 3.0% of cane sugar, 1.0% of monosodium glutamate, 3.0% of cooking wine, 2.0% of onion essential oil and 0.1% of edible acetic acid. Mixing the processed pork fillet with the curing agent, stirring, and curing for 30 min.
(3) Coating powder for frying the prepared food: 30% of wheat flour, 20% of corn starch, 20% of resistant starch, 4% of soybean protein isolate, 2% of baking powder, 10% of soybean oil and 0.5% of carrageenan (the mass ratio of powder to water is 1: 1).
(4) Carrying out air frying: frying at 160 deg.C for 16 min.
The quality of the fried crispy meat of the described protocol was determined as follows:
1. measurement of color of outer Shell
The difference in skin color was measured using a spectrocolorimeter and expressed as a brightness value (L), a redness value (a), and a yellowness value (b).
TABLE 1 comparison of color of the crispy meats obtained according to the different protocols (n ═ 3)
Figure BDA0003632700010000051
Note: the lower case letters in the same column are different and show significant difference (P < 0.05), and the table is as follows; n-3 represents three parallel experiments.
The color directly affects the food desire of consumers, and as can be seen from table 1, the schemes one to three belong to the traditional oil bath type frying, compared with the scheme four-air frying, the group L has no significant difference, the scheme four a has significant increase (P < 0.05), and the a can show better golden yellow. The second to fourth b values were not significantly different, indicating that the fourth group, which was air fried, was better in color than the conventional frying.
2. Determination of Water and oil content
The moisture content is determined by direct drying method according to GB 5009.3-2016 (determination of moisture in food safety national standard food). The crude fat content is determined by extracting oil by Soxhlet extraction method, and determining the crude fat content according to GB 5009.6-2016 (determination of fat content in food safety national standard food).
TABLE 2 comparison of moisture content and oil content of the meat crisps obtained according to different protocols
Figure BDA0003632700010000061
The amount of oil used for frying is mainly different from that of the conventional oil bath frying, and as can be seen from table 2, after resistant starch is used to replace common corn starch in the third scheme, the third scheme has significant difference from the first and second schemes, the water retention is significantly enhanced, the fourth scheme has serious water loss in air frying, and has significant difference from the first to third schemes, and the oil content is significantly reduced (P is less than 0.05).
3. Determination of acrylamide content
Preparation of acrylamide standard curve: accurately sucking 1mL of acrylamide solution with mass concentration of 1.0mg/mL, adding ultrapure water to fix the volume to 100mL, and obtaining acrylamide standard solution with mass concentration of 10 mu g/mL; taking 2, 5, 10, 15, 20 and 25mL of the standard solution respectively, adding ultrapure water into a 100mL brown volumetric flask for constant volume, preparing solutions with acrylamide mass concentrations of 0.2, 0.5, 1.0, 1.5, 2.0 and 2.5 mu g/mL respectively, filtering with a 0.22 mu m water-based filter membrane, and placing in a sample bottle for later use.
Extraction of acrylamide: taking 5g of homogenized small crispy meat, placing the small crispy meat in a 50mL centrifuge tube, adding a certain amount of n-hexane, degreasing for 2 times, and drying by a nitrogen blowing instrument; then adding 20mL of ultrapure water, and carrying out ultrasonic extraction for 15 min; adding 500 μ L of Carrez I and Carrez II solution, oscillating for 5min, centrifuging at 4 deg.C and 10000 r/min for 10min, collecting middle layer solution, and filtering with 0.22 μm water system filter membrane.
Chromatographic conditions are as follows: the chromatographic column is a C18 column, and the ratio of mobile phase: methanol to water 5: 95 (V/V); the flow rate is 0.6 mL/min; the sample size is 10 mu L; the column temperature is 30 ℃; automatic sample introduction; the ultraviolet detection wavelength is 210 nm.
Table 3 variation of acrylamide content in the crisps obtained according to the different protocols (n ═ 3)
Figure BDA0003632700010000071
Note: the first scheme is a control group; n-3 represents three parallel experiments.
As can be seen from the table, the acrylamide content of the fried crisp meat prepared by the method can reach 80 percent.
4. Storage experiment of fried crisp meat
Selecting finished products of the small crisp meat according to the schemes one to four, carrying out vacuum packaging by using a common self-sealing bag, sealing completely, putting into a laboratory refrigeration house, storing at low temperature of 0-4 ℃ in a dark place for 8 months, and carrying out sensory evaluation on the fried small crisp meat.
TABLE 4 Fried crisp meat sensory score criteria
Figure BDA0003632700010000072
TABLE 5 evaluation results of meat sensory evaluation of Fried cutlets
Figure BDA0003632700010000081
From the sensory evaluation result of the crisp meat, after 8 months of low-temperature and dark storage, the first, second and third schemes of oil bath frying obviously have high oil content, and are easy to decay and deteriorate to generate rancid flavor. In the fourth scheme, the crispy meat fried by the air fryer has low greasy feeling, relatively light quality change in the storage process and highest sensory score.
It is to be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Those skilled in the art will recognize that the basic inventive concepts and variations and modifications can be made to the embodiments described herein, and that the invention is intended to cover all modifications, equivalents and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

Claims (9)

1. A method for reducing the acrylamide content in the small crisp meat is characterized by comprising the following steps,
the method comprises the following steps: mixing the processed pork fillet with curing agent, and curing;
step two: wrapping the pickled pork fillet with wrapping powder for fried food;
step three: air frying the flour-coated pork fillet.
2. The method of reducing the acrylamide content of a crisp meat of claim 1 wherein the curing agent comprises the following ingredients in parts by weight: 1-2 parts of salt, 3-4 parts of cane sugar, 1-2 parts of monosodium glutamate, 3-5 parts of cooking wine and 1-3 parts of exogenous additives.
3. The method for reducing the acrylamide content in the crispy cutlet as claimed in claim 2, wherein the exogenous additive is onion essential oil and edible acetic acid, and the edible acetic acid is added after being diluted by 100 times, and the mixing ratio is 1-2: 1.
4. The method of reducing the level of acrylamide in a crisp pork according to claim 1, wherein the treatment of the pork fillet comprises removing visible fat and connective tissue from the surface of the pork fillet, and the pork fillet is about 4cm x 1cm x 0.8cm after the treatment.
5. The method for reducing the acrylamide content in a crispy cutlet according to claim 1, wherein the coating powder for fried food comprises, in parts by weight: 60 parts of wheat flour, 40-80 parts of corn starch, 20-40 parts of resistant starch, 8 parts of soybean protein isolate, 4 parts of baking powder, 20 parts of soybean oil and 1 part of carrageenan.
6. The method for reducing the acrylamide content of a crisp snack of claim 5 wherein the corn starch to resistant starch ratio of the breading for fried food is 1-2: 1.
7. The method of reducing the acrylamide content of a crisp meat of claim 5 wherein the resistant starch is resistant starch RS 4.
8. The method for reducing the acrylamide content in the crispy cutlet according to claim 1, wherein the air frying temperature is 150 to 180 ℃ and the frying time is 15 to 18 min.
9. The method of reducing the acrylamide content in a crispy cutlet of claim 1, wherein the marinating time is 30 min.
CN202210495207.5A 2022-05-07 2022-05-07 Method for reducing acrylamide content in small crispy meat Pending CN115053945A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115500479A (en) * 2022-10-08 2022-12-23 上海博朝餐饮有限公司 Nutrient and healthy secret pork chop and preparation method thereof
CN115500462B (en) * 2022-09-21 2024-03-22 江苏宝立食品科技有限公司 Sweet and sour ridge paste powder and preparation method thereof

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