CN115041309A

CN115041309A - Biological gene tissue sample centrifugal sampling system

Info

Publication number: CN115041309A
Application number: CN202210986929.0A
Authority: CN
Inventors: 张金银; 巩赞博; 巩赞斌; 巩赞华; 黄咏华; 彭劼
Original assignee: Huachenyang Shenzhen Technology Co ltd
Current assignee: Huachenyang Shenzhen Technology Co ltd
Priority date: 2022-08-17
Filing date: 2022-08-17
Publication date: 2022-09-13
Anticipated expiration: 2042-08-17
Also published as: CN115041309B

Abstract

The invention relates to a biological gene tissue sample centrifugal sampling system, which relates to the technical field of sample detection centrifugation and comprises a control box and a sealing door hinged to the left side of the upper end of the control box, wherein a centrifugal part for carrying out centrifugal operation on a test tube for placing biological gene tissue is arranged in the control box, and a sucking disc fixer for fixing the test tube is arranged on the inner side of the sealing door; the invention can carry out centrifugal operation on the biological gene tissue sample in a closed environment, and avoids the pollution caused by long-time contact with air when the biological gene sample is placed; secondly, the invention can collect the biological gene sample tissue after centrifugal stratification in a sealed state, and can carry out sealing treatment and sealing storage on the collected biological gene sample tissue.

Description

Biological gene tissue sample centrifugal sampling system

Technical Field

The application relates to the technical field of sample detection centrifugation, in particular to a biological gene tissue sample centrifugal sampling system.

Background

Biological samples generally refer to flowers, leaves, stems, roots, seeds, etc. of plants, body fluids (e.g., urine, blood, saliva, bile, gastric juice, lymph fluid and other secretions of organisms, etc.) of animals (including humans), hair, muscle and some tissue organs (e.g., thymus, pancreas, liver, lung, brain, stomach, kidney, etc.), and various microorganisms.

Biological tissue samples are generally used in some genetic testing experiments, and are obtained by subjecting a biological tissue sample to centrifugation after being clarified by physiological saline and other auxiliary reagents, wherein the purpose of centrifugation is to separate substances having different densities and to allow the substances to be layered, thereby purifying the desired substances.

In the existing biological gene tissue sample centrifugal sampling system, for example, chinese patent with patent number CN214487391U, a centrifugal device for gene detection is disclosed, which includes a third limiting plate, the upper surface of the third limiting plate is fixedly connected with a second driving motor, the output end of the second driving motor is fixedly connected with a transmission shaft, one end of the transmission shaft is fixedly connected with a first bearing plate, the other end of the transmission shaft is fixedly connected with a second bearing plate, a supporting base is arranged below the third limiting plate, both ends of the upper surface of the supporting base are fixedly connected with a fourth limiting plate slidably connected with the third limiting plate, and the lower surface of the third limiting plate is fixedly connected with a buffer air bag corresponding to the supporting base; its simple structure, convenient to use can highly carry out the automation according to user's in-service use demand to the protection casing during the use and adjust to prevent that protection casing and test tube from colliding and causing the test tube to damage, promote the user and experience centrifugal device's use.

In the above-described prior art, although the sample in the test tube can be centrifuged, it cannot change the angle at which the test tube is centrifuged, and the stability of the test tube at the time of centrifugation is poor.

Secondly, in the prior art, after the centrifugation of the sample in the test tube is finished, the syringe is usually used to extract the liquid in the test tube, and when the needle of the syringe repeatedly punctures the test tube plug and collects the layered liquid in the test tube, the needle of the syringe is easily contaminated with the liquid samples of different layers, so that the liquid samples are mixed, and the accuracy of the procedure detection is affected.

Disclosure of Invention

In order to enable the liquid in the test tube to be quickly and accurately sealed and collected in a sealed state, the application provides a biological gene tissue sample centrifugal sampling system.

The utility model provides a biological gene tissue sample centrifugation sampling system, includes control box and the articulated sealing door in control box upper end left side, the inside of control box is equipped with and is used for carrying out the centrifugal part of centrifugal operation to the test tube of placing biological gene tissue, and the inboard of sealing door is equipped with carries out the sucking disc fixer fixed to the test tube stopper.

The centrifugal part comprises a centrifugal motor fixed at the bottom end inside the control box, a disc is installed at the upper end of the centrifugal motor and used for placing test tubes of biological genetic tissues, a placing part used for fixing the test tubes is arranged at the upper end of the disc, and a layered absorbing mechanism used for centrifugally sampling samples in the test tubes is arranged on the placing part.

Preferably, the portion of placing includes that the disc upper end is used for placing the arc of test tube along its circumference direction equidistant articulated four groups, has seted up the arc recess on the lateral wall of arc, has seted up the bar groove in the arc recess for place the test tube, one side that the arc is close to the arc recess is fixed with carries out spacing fixed lantern ring to the test tube, and the bottom in the arc outside is equipped with carries out the bearing board of bearing to the test tube, and the inside wall of arc is equipped with the regulating part.

Preferably, the regulating part includes the central post that disc upper end middle part is fixed, and the cover that slides from top to bottom is equipped with the regulation lantern ring, and the lateral wall of adjusting the lantern ring articulates along its circumference equidistant has the regulation pole, adjusts the pole and keeps away from the one end of adjusting the lantern ring and articulate each other with the arc, and the lower extreme of adjusting the lantern ring is connected with electric putter, and electric putter fixes the upper end at the disc.

Preferably, the sucking disc fixer includes that the sealing door upper end passes through threaded connection's the threaded rod of pressing down, and the lower extreme of pressing down the threaded rod rotates through the bearing and is connected with trapezoidal post, and the upper end of trapezoidal post is connected with spacing flexible post with the lower extreme of sealing door, and the equidistant expanding spring pole that is fixed with of circumference direction along its axle center on the inclined plane lateral wall of trapezoidal post, and the one end fixedly connected with that trapezoidal post was kept away from to the expanding spring pole is used for the sucking disc of fixed test tube sealing plug, is equipped with the executive that can initiatively loosen the test tube sealing plug on the sucking disc.

Preferably, the executive component comprises a ventilating pipe which is fixedly penetrated by the end, close to the telescopic spring rod, of the sucker, an ventilating column is connected in the ventilating pipe in a sliding mode through a sealing ring, a ventilating groove is formed in one side, far away from the ventilating pipe, of the lower end of the ventilating column, the same ventilating groove as that of the ventilating column is formed in one side, close to the sucker, of the lower end of the ventilating column, and the ventilating column is fixed on the side wall of the trapezoidal column.

Preferably, the mechanism is absorb including the activity sets up the L shape on the test tube lateral wall in the layering, the equal fixedly connected with board of lateral wall around the L shape is absorbed the frame, the left side of a board is through centre gripping spring beam fixedly connected with grip block, the L shape is absorbed the one end that the grip block was kept away from to the frame and is equidistantly offered a plurality of from the top down distribution's liquid extraction hole, the downthehole drain pipe that is fixed with of liquid extraction, the chute has been seted up to the inside downside of drain pipe, it has sealed post to slide about in the drain pipe, the one end that the drain pipe was kept away from to sealed post links to each other with the closing plate, one side that sealed post was kept away from to the closing plate is connected with the inner wall that the L shape was absorbed the frame through extrusion spring beam, be equipped with the module that opens and shuts to the liquid extraction hole on the L shape is absorbed the frame.

The upper end of the sealing plate is fixed with a horizontal plate, and the horizontal plate is hinged with two symmetrically distributed diagonal rods.

Preferably, the module that opens and shuts includes that L shape absorbs frame inner wall both ends pivoted pivot from top to bottom, it has the area that opens and shuts of two front and back symmetries to rotate jointly in two pivots, it has the removal frame to be located to open and shut the lateral wall in the area of the inside rear side of L shape absorption frame through the round pin hub fixation, it runs through the flexible piece to remove the one end that the frame far away left the area, the flexible piece is located to remove one side conflict of frame inside and has three hornblocks, and be connected with the executive spring between the inner wall of flexible piece and removal frame, the three hornblocks slide the spacing inslot on removing the frame inner wall from top to bottom, the upper end of three hornblocks is connected with the hank rope, the cover is equipped with reset spring between three hornblocks and the removal frame inner wall, and reset spring overlaps and establishes on the hank rope.

The upper end of L shape ingestion frame is rotated and is had a knob, and the lower extreme of a knob passes through the winding threaded rod through the bearing rotation, and the winding threaded rod links to each other with the hank rope and the hank rope winding is on the winding threaded rod.

The outer side wall of the L-shaped ingestion frame is rotated with a second knob, and the side wall of the second knob is connected with the rotating shaft through a bearing.

And a sample collecting assembly is arranged on the opening and closing belt positioned on the front side in the L-shaped ingestion frame.

Preferably, the sample collection subassembly includes that L shape absorbs on the frame inner wall and is located the spout that sets up under the sealing plate, slidable mounting has the collection frame in the spout, the upper end fixed mounting of collection frame has the apron No. one, flexible groove has been seted up on the lateral wall of apron No. one, there is the apron No. two through sealing spring slidable mounting in the flexible inslot, one side that the apron was kept away from to the upper end of No. two apron is fixed with the passive piece, the U-shaped frame is installed to the lower extreme of sealing plate, the middle part of U-shaped frame is slided in the front and back to the passive piece that No. two apron upper ends are fixed.

Preferably, be located be fixed with the vertical plate on the lateral wall in the area that opens and shuts of the inside front side of L shape ingestion frame, the side of vertical plate is connected with the conflict spring, one side fixedly connected with T shape frame that the vertical plate was kept away from to the conflict spring, sliding connection has the right angle trapezoidal piece of seting up the T-slot on the T shape frame, the inclined plane of right angle trapezoidal piece distributes towards the direction of closing plate, the lower extreme of right angle trapezoidal piece is the rack structure, the lower extreme meshing of right angle trapezoidal piece has the gear, the lower extreme meshing of gear has fixed rack, the one end that fixed rack is close to the collection frame is connected with L shape frame.

The lower end of the collecting frame is fixed with a metal plate capable of being magnetically attracted, the upper end of the L-shaped frame is connected with a magnetic sheet, and the lower end of the magnetic sheet is provided with an electromagnetic coil to be electrified.

The side wall of the L-shaped shooting frame, close to the sealing plate, is fixed with turning blocks at equal intervals, and the turning blocks are of a right-angle trapezoidal structure.

The lower end of the inclined rod is abutted with an L-shaped rod, the L-shaped rod penetrates through the horizontal plate in a sliding mode, and the part, located at the upper end of the horizontal plate, of the L-shaped rod is connected with a supporting spring rod.

Preferably, four round holes have been seted up to the position that the upper end of sealing door corresponds the test tube, and the inside of sealing door is hollow structure, and the inboard rotation of sealing door has the ring, sets up the dislocation hole that corresponds with four round holes on the ring, and the meshing has adjusting gear on the lateral wall of ring, and there is adjust knob upper end of adjusting gear through center pin connection, and adjust knob rotates the upper end at the sealing door.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the invention can carry out centrifugal operation on the biological gene tissue sample in a closed environment, and avoid the situations of pollution and the like when the biological gene sample is placed.

2. The device can collect the liquids in the test tubes which are layered after centrifugation one by one, avoid the cross mixing between the liquids to cause the pollution of the whole sample, and meanwhile, when the layered biological gene tissue sample is collected, the device can be in a closed state to avoid the pollution of the sample in the air.

3. The invention can collect the biological gene sample tissue after centrifugal stratification in a sealed state, and can carry out sealing treatment and sealing storage on the collected biological gene sample tissue.

Drawings

Fig. 1 is a first perspective structural view of the main body of the present invention.

Fig. 2 is a second perspective structural view of the main body of the present invention.

Fig. 3 is a partial structural schematic view of the centrifugal part of the present invention.

Fig. 4 is a partial structural view of the suction cup holder of the present invention.

Fig. 5 is a partial schematic view of the internal structure of the sealing door of the present invention.

Fig. 6 is a partial structural schematic diagram of an actuator in the present invention.

Fig. 7 is a partial structural schematic view of the placing section.

Fig. 8 is a first perspective structure diagram of the layered ingestion mechanism.

Fig. 9 is a second perspective structural view of the layered ingestion mechanism.

Fig. 10 is a second perspective view of the layered ingestion mechanism.

Fig. 11 is a partial enlarged view of the invention at C in fig. 10.

Fig. 12 is a first perspective structural diagram of the opening and closing module.

Fig. 13 is a second perspective structural view of the opening and closing module.

Fig. 14 is a third view structure diagram of the opening and closing module.

Fig. 15 is a schematic view of a first perspective configuration of the sample collection assembly.

Fig. 16 is a schematic view of a second perspective configuration of the sample collection assembly.

Fig. 17 is a schematic view of a third perspective configuration of the sample collection assembly.

Description of reference numerals: A. a test tube; B. a test tube plug; 1. a control box; 2. a sealing door; 3. a centrifugal part; 4. a suction cup holder; 30. a centrifugal motor; 31. a disc; 32. a placement section; 33. a layered uptake mechanism; 320. an arc-shaped plate; 321. a fixed collar; 322. a bearing plate; 34. an adjustment member; 340. a central column; 341. adjusting the lantern ring; 342. adjusting a rod; 343. an electric push rod; 40. pressing the threaded rod; 41. a trapezoidal column; 42. limiting the telescopic column; 43. a telescoping spring rod; 44. a suction cup; 45. an executive component; 450. a breather pipe; 451. ventilating columns; 452. a vent channel; 330. an L-shaped intake frame; 331. a first board; 332. clamping the spring rod; 333. a clamping plate; 334. a liquid outlet pipe; 335. a chute; 336. sealing the column; 325. a sealing plate; 337. extruding the spring rod; 35. an opening and closing module; 338. a horizontal plate; 339. a diagonal bar; 350. a rotating shaft; 351. opening and closing the belt; 352. moving the frame; 353. a telescopic block; 354. a triangular block; 355. stranding a rope; 356. a return spring; 357. an actuator spring; 358. a first knob; 359. winding a threaded rod; 360. a second knob; 37. a sample collection assembly; 370. a collection frame; 371. a first cover plate; 372. a seal spring; 373. a second cover plate; 374. a passive block; 375. a U-shaped frame; 380. a vertical plate; 381. against the spring; 382. a T-shaped frame; 383. a right-angled trapezoidal block; 384. a gear; 385. fixing a rack; 386. an L-shaped frame; 387. a metal plate; 388. a magnetic sheet; 390. a turning block; 391. an L-shaped rod; 392. a support spring rod; 460. a circular ring; 461. an adjusting gear; 462. adjusting a knob; 463. a central axis.

Detailed Description

The present application is described in further detail below with reference to figures 1-17.

The embodiment of the application discloses a biological gene tissue sample centrifugal sampling system, which can be used for hermetically collecting a test tube A which is centrifuged and layered, and avoiding the phenomenon that the interior of the test tube A is mutated or polluted by the outside due to the long-time contact between the sample in the test tube A and the outside air after centrifugation.

Referring to fig. 1 and 2, a biological gene tissue sample centrifugal sampling system, including

control box

1 and 1 upper end left side articulated sealing door 2 of control box, the inside of control box 1 is equipped with and is used for carrying out centrifugal part 3 of centrifugal operation to the test tube A who places biological gene tissue, the inboard of sealing door 2 is equipped with carries out fixed sucking disc fixer 4 to test tube stopper B, sucking disc fixer 4's effect is fixed test tube A and test tube stopper B, centrifugal part 3's effect is to carrying out quick centrifugal operation to test tube A.

Referring to fig. 2, centrifugation part 3 includes the inside bottom mounting's of control box 1 centrifugation motor 30, disc 31 is installed to the upper end of centrifugation motor 30, be used for placing biological gene tissue's test tube A, the upper end of disc 31 is equipped with the portion of placing 32 that is used for fixed test tube A, be equipped with the layering after carrying out the centrifugation sample to the sample in the test tube A on the portion of placing 32 and intake mechanism 33, the effect of portion of placing 32 is the required device that is used for placing test tube A, the effect that intake mechanism 33 stratified can be collected it under sealed state after carrying out the centrifugation layering in order to make the liquid in the test tube A, avoid taking place the mixture between the liquid on every layer, thereby pollute the sample.

Referring to fig. 3, before centrifuging the biological sample, the test tube a loaded with the biological sample is first sterilized, then loaded with auxiliary reagents required for centrifugation, and finally placed in the placing part 32 for fixation; the placing part 32 comprises four groups of arc plates 320 which are used for placing the test tube A and are hinged along the circumferential direction of the disc 31 at equal intervals, an arc groove is formed in the outer side wall of each arc plate 320, a strip-shaped groove is formed in each arc groove and used for placing the test tube A, one side, close to each arc groove, of each arc plate 320 is fixed with a fixed lantern ring 321 which limits the test tube A, the bottom of the outer side of each arc plate 320 is provided with a bearing plate 322 which supports the test tube A, and the inner side wall of each arc plate 320 is provided with an adjusting piece 34.

In the specific embodiment, after adding the required auxiliary reagent of centrifugation in test tube A, the mechanism 33 is absorb to the layering after the disinfection inserts on test tube A inner wall, operating personnel places test tube A and passes fixed lantern ring 321 next, later contradict test tube A to the upper end of the bearing board 322 of the inside of control box 1 after the disinfection, and it conflicts at the bar inslot to absorb the mechanism 33 to the layering, it is required to explain that, all laid the rubber layer on bearing board 322 and the fixed lantern ring 321, be used for playing absorbing effect, avoid centrifugal in-process test tube A to take place the damage.

Referring to fig. 4 and 5, after the test tube a is fixed, the sealing cover required by the test tube a also needs to be fixed in the control box 1, as shown below, the suction cup fixer 4 includes a pressing threaded rod 40 connected to the upper end of the sealing door 2 through a thread, the lower end of the pressing threaded rod 40 is rotatably connected to a trapezoidal column 41 through a bearing, and the upper end of the trapezoidal column 41 and the lower end of the sealing door 2 are connected to a limiting telescopic column 42; the position of the trapezoidal column 41 is adjusted by rotating the pressing threaded rod 40, so that the trapezoidal column 41 moves up and down, and the limit telescopic column 42 functions to prevent the trapezoidal column 41 from rotating while the pressing threaded rod 40 is rotated.

The inclined side wall of the trapezoidal column 41 is fixed with telescopic spring rods 43 at equal intervals along the circumferential direction of the axis center, one end of each telescopic spring rod 43 far away from the trapezoidal column 41 is fixedly connected with a sucker 44 used for fixing a sealing plug B of a test tube A, the sealing plug B of the test tube A can be adsorbed on the sucker 44, and an executing piece 45 capable of actively loosening the sealing plug B of the test tube A is arranged on the sucker 44.

In the specific embodiment, after the test tube a is placed inside the control box 1, the back of the sealing plug B is abutted to the suction cup 44, after the air in the suction cup 44 is exhausted, the sealing plug B is fixed to the suction cup 44, the sealing plug B is fixed to the lower end of the sealing door 2, the sealing door 2 is covered on the upper end of the control box 1, and the whole control box 1 is in a closed state.

Referring to fig. 5 again, four round holes are opened at the position of the upper end of the sealing door 2 corresponding to the test tube a, the inside of the sealing door 2 is of a hollow structure, the inner side of the sealing door 2 rotates to form a ring 460, the ring 460 is provided with staggered holes corresponding to the four round holes, the outer side wall of the ring 460 is meshed with an adjusting gear 461, the upper end of the adjusting gear 461 is connected with an adjusting knob 462 through a central shaft 463, and the adjusting knob 462 rotates at the upper end of the sealing door 2.

In the specific embodiment, after the required sealed lid of test tube A and test tube A all was placed the inside of control box 1, operating personnel covered sealing door 2, and the preparation work before centrifugation was accomplished this moment, and its main effect is in sealed state completely in order to guarantee the centrifugal in-process, avoids the dust pollution test tube A in the air.

After the biological sample is simply handled, operating personnel rotates adjust knob 462, and adjust knob 462 passes through adjusting gear 461 and drives ring 460 and rotate for dislocation hole on the ring 460 coincides with the round hole on sealing door 2, and the biological sample after will handling passes through the round hole after that, puts into test tube A, and reverse rotation adjust knob 462 once more this moment makes ring 460 cover the upper end at the round hole again, makes control box 1 be in inclosed spatial state.

Referring back to fig. 3, in the initial state, the arc plate 320 for placing the test tube a is in a vertical state with respect to the ground, and the test tube a is also in a vertical state when placing the biological sample, and after the biological sample is placed inside the test tube a, the adjusting member 34 adjusts the angle of the test tube a, thereby improving the centrifugation efficiency, as follows:

the adjusting part 34 comprises a central column 340 fixed in the middle of the upper end of the disc 31, an adjusting lantern ring 341 is sleeved on the central column 340 in a vertically sliding manner, an adjusting rod 342 is hinged to the outer side wall of the adjusting lantern ring 341 along the circumferential direction of the outer side wall at equal intervals, one end, far away from the adjusting lantern ring 341, of the adjusting rod 342 is hinged to the arc-shaped plate 320, an electric push rod 343 is connected to the lower end of the adjusting lantern ring 341, and the electric push rod 343 is fixed to the upper end of the disc 31.

In the specific embodiment, electric putter 343 starts, and electric putter 343 pulling is adjusted lantern ring 341 and is removed downwards, adjusts lantern ring 341 and removes downwards in-process and drives test tube A through adjusting pole 342 and slide around its bottom for test tube A is in the state of slope, and test tube A's opening distributes inwards this moment, and when it was centrifuged, it not only can improve test tube A's stability, avoids test tube A to take place vibrations, can improve centrifugal efficiency moreover.

Referring to fig. 6, after the position of the test tube a is adjusted, the test tube a needs to be sealed, the actuator 45 includes a vent pipe 450 through which one end of the suction cup 44 close to the retractable spring rod 43 penetrates and is fixed, a vent column 451 is slidably connected in the vent pipe 450 through a seal ring, a vent groove 452 is formed in one side of the lower end of the vent column 451, which is far away from the vent pipe 450, a vent groove 452 identical to that of the vent column 451 is formed in one side of the lower end of the vent pipe 450 close to the suction cup 44, and the vent column 451 is fixed on the side wall of the trapezoidal column 41.

In the specific embodiment, an operator rotates and presses the threaded rod 40, presses the threaded rod 40 to drive the trapezoidal column 41 to move downwards, drives the sealing plug B to move obliquely in the process that the trapezoidal column 41 moves downwards until the sealing plug B is inserted into the tube opening of the test tube a, then, the pressing screw rod 40 is rotated continuously, so that the vent cylinder 451 on the trapezoidal cylinder 41 is inserted into the vent pipe 450, when the vent post 451 moves a certain distance in the vent tube 450, the vent groove 452 of the vent post 451 and the vent groove 452 of the vent tube 450 overlap each other, the inside of the suction cup 44 is connected to the atmosphere through the vent groove 452, air enters the position where the suction cup 44 contacts the sealing plug B, the suction cup 44 loses suction, the sealing plug B covers the test tube a, and the suction cup 44 is separated from the sealing plug B, so that the test tube a and the sealing plug B do not touch the suction cup 44 and the trapezoidal column 41 when centrifugally rotating.

Referring to fig. 7, 8 and 9, after the test tube a is sealed by the sealing plug B, the centrifugal motor 30 drives the disc 31 and the test tube a whose upper end is fixed to rotate in a telling manner, after a period of time, the centrifugation is completed, the biological sample in the test tube a is located at the bottom of the test tube a, the liquid in the test tube a is layered, the layered liquid is accurately ingested by the layered ingesting mechanism 33, the mixing between the liquids at different layers is avoided, the pollution is caused, and when the liquid is harvested, the long-time contact with the air is avoided, the pollution or the variation is caused, and the liquid is ingested in a closed environment.

The layered ingestion mechanism 33 comprises an L-shaped ingestion frame 330 movably arranged on the side wall of the test tube A, the front side wall and the rear side wall of the L-shaped ingestion frame 330 are both fixedly connected with a first plate 331, and the left side of the first plate 331 is fixedly connected with a clamping plate 333 through a clamping spring rod 332; when intaking the L shape and putting test tube A's lateral wall into frame 330 and fixing, intake the inside that frame 330 inserted test tube A with the L shape, and need can place the L shape after the supplementary test tube A with test tube A in matches well and intake frame 330, and the grip block 333 that L shape was intake on the frame 330 this moment contradicts on test tube A's lateral wall through centre gripping spring pole 332, make the L shape intake the frame 330 through grip block 333 this moment and fix in test tube A's inside, avoid centrifugal in-process L shape to intake frame 330 and take place the displacement.

Referring to fig. 10, a plurality of liquid-extracting holes distributed from top to bottom are formed at equal intervals at one end of the L-shaped intake frame 330 away from the clamping plate 333, a liquid outlet pipe 334 is fixed in each liquid-extracting hole, and an inclined groove 335 is formed at the lower side of the inside of the liquid outlet pipe 334; after layering appears in test tube A, collect the liquid of different levels from top to bottom in proper order, and liquid goes out through drain pipe 334 is controlled through opening and shutting module 35 to the liquid, and liquid is collected through sample collection subassembly 37 after flowing out through drain pipe 334.

Sliding about in the drain pipe 334 has sealed post 336, and the one end that drain pipe 334 was kept away from to sealed post 336 links to each other with the closing plate 325, and one side that sealed post 336 was kept away from to closing plate 325 is connected with the inner wall of L shape ingestion frame 330 through extrusion spring pole 337, is equipped with the module 35 that opens and shuts to the drawing liquid hole on the L shape ingestion frame 330.

Referring to fig. 11, 12 and 13, the opening and closing module 35 includes two rotating shafts 350 rotating on the upper and lower ends of the inner wall of the L-shaped intake frame 330, two opening and closing belts 351 symmetrical front and back rotate together on the two rotating shafts 350, a movable frame 352 is fixed on the side wall of the opening and closing belt 351 located on the rear side inside the L-shaped intake frame 330 through a pin, an expansion block 353 penetrates through one end of the movable frame 352 far away from the opening and closing belt 351 in a sliding manner, a triangular block 354 abuts against one side of the expansion block 353 located inside the movable frame 352, an execution spring 357 is connected between the expansion block 353 and the inner wall of the movable frame 352, the triangular block 354 slides up and down in a limiting groove on the inner wall of the movable frame 352, a twisted rope 355 is connected to the upper end of the triangular block 354, a return spring 356 is sleeved between the inner wall of the movable frame 352 and the triangular block 354, and the return spring 356 is sleeved on the twisted rope 355.

A first knob 358 is rotated at the upper end of the L-shaped ingestion frame 330, a winding threaded rod 359 penetrates through the lower end of the first knob 358 in a rotating mode through a bearing, the winding threaded rod 359 is connected with a strand 355, and the strand 355 is wound on the winding threaded rod 359.

Referring to fig. 14 and 15, a second knob 360 is rotatably mounted on an outer side wall of the L-shaped capturing frame 330, and a side wall of the second knob 360 is connected to the rotating shaft 350 through a bearing.

The upper end of the sealing plate 325 is fixed with a horizontal plate 338, and the horizontal plate 338 is hinged with two symmetrically distributed oblique rods 339.

The sample collection assembly 37 is provided on the opening and closing belt 351 located on the front side inside the L-shaped intake frame 330.

In the embodiment, after the biological sample in the test tube a is centrifuged, the sealing door 2 is opened to take out the test tube a in a sealed state, the L-shaped intake frame 330 is always fixed in the test tube a, the test tube a is then fixed, the second knob 360 is rotated, the second knob 360 drives the opening and closing belt 351 to rotate through the rotating shaft 350, and the moving frame 352 fixed on the opening and closing belt 351 moves up and down synchronously.

In an initial state, the moving frame 352 is located at the uppermost end of the L-shaped intake frame 330, when liquid after centrifugal layering needs to be taken, the second knob 360 is rotated, the moving frame 352 starts to move downwards, when the moving frame 352 moves to a position above a liquid drawing hole corresponding to the bottom of the uppermost layer of liquid in the test tube a, the first knob 358 is rotated, the first knob 358 drives the winding threaded rod 359 to rotate, the winding threaded rod 359 rotates and simultaneously drives the stranded rope 355 to wind, the lower end of the stranded rope 355 starts to contract upwards until the stranded rope 355 pulls the triangular block 354 to move upwards, the telescopic block 353 abutting against the inclined plane of the triangular block 354 is pressed in the upward moving process of the triangular block 354, and the telescopic block 353 extends outwards along the moving frame 352 until the telescopic block 353 is located right above the inclined rod 339 on the horizontal plate 338.

When the expansion block 353 is not extended, the movable frame 352 has a certain distance from the sealing plate 325 and the horizontal plate 338, the movable frame 352 moves up and down without contacting the sealing plate 325 and the horizontal plate 338, and when the expansion block 353 is extended, the expansion block 353 contacts the sealing plate 325, the horizontal plate 338 and the inclined bar 339.

After the telescopic block 353 extends out, the second knob 360 continues to rotate, so that the moving frame 352 moves downwards until the telescopic block 353 abuts against the inclined rod 339, the inclined rod 339 starts to rotate downwards after being extruded, one end of the inclined rod 339 abuts against the inner wall of the L-shaped ingestion frame 330 and cannot move, the other end of the inclined rod 339 drives the sealing plate 325 and the sealing column 336 to move towards the direction away from the liquid outlet hole until the sealing column 336 moves a certain distance away from the liquid outlet hole from the inside of the liquid outlet pipe 334, the inclined groove 335 on the liquid outlet pipe 334 is exposed, and at the moment, liquid enters the collecting frame 370 arranged below the liquid outlet hole through the liquid outlet hole and the inclined groove 335 to be uniformly collected.

Referring again to FIGS. 11, 12 and 13, the device for collecting and sealing the liquid of a biological sample after centrifugation and stratification of the biological sample is shown as follows:

the sample collecting assembly 37 comprises a sliding groove which is arranged on the inner wall of the L-shaped shooting frame 330 and is positioned under the sealing plate 325, a collecting frame 370 is arranged in the sliding groove in a sliding manner, a first cover plate 371 is fixedly arranged at the upper end of the collecting frame 370, a telescopic groove is formed in the side wall of the first cover plate 371, a second cover plate 373 is arranged in the telescopic groove in a sliding manner through a sealing spring 372, a driven block 374 is fixed on one side, away from the first cover plate 371, of the upper end of the second cover plate 373, a U-shaped frame 375 is arranged at the lower end of the sealing plate 325, and the driven block 374 fixed at the upper end of the second cover plate 373 slides back and forth in the middle of the U-shaped frame 375; the passive block 374 is slidably disposed at the middle of the U-shaped frame 375 and can be separated from the U-shaped frame 375.

In the embodiment, when the telescopic block 353 on the moving frame 352 downwardly presses the inclined rod 339, the inclined rod 339 drives the sealing plate 325 to move in the direction away from the liquid outlet hole, and thus, the U-shaped frame 375 at the upper end of the sealing plate 325 pushes the driven block 374 to synchronously move, the driven block 374 drives the second cover plate 373 to open when moving, and at this time, the opening at the upper end of the collecting frame 370 is opened, so that the sample liquid flowing out from the liquid outlet hole can be collected.

In the initial state, the second cover plate 373 is extended out of the telescopic groove of the first cover plate 371 by the elastic force of the sealing spring 372, and at this time, the first cover plate 371 and the second cover plate 373 cover the opening at the upper end of the collecting frame 370, so that a sealed space is formed.

Referring to fig. 16 and 17, a vertical plate 380 is fixed on a side wall of the opening and closing belt 351 located at the front side inside the L-shaped ingestion frame 330, a contact spring 381 is connected to a side end of the vertical plate 380, a T-shaped frame 382 is fixedly connected to a side of the contact spring 381 far away from the vertical plate 380, and a right-angled trapezoidal block 383 provided with a T-shaped groove is slidably connected to the T-shaped frame 382; in the initial state, the spring 381 abuts against the inner wall of the L-shaped intake frame 330 by abutting against the spring 381, and can slide up and down along the inner wall of the L-shaped intake frame 330.

The inclined surfaces of the right-angle trapezoidal blocks 383 are distributed towards the direction of the sealing plate 325, the lower ends of the right-angle trapezoidal blocks 383 are in a rack structure, the lower ends of the right-angle trapezoidal blocks 383 are meshed with a gear 384, the lower ends of the gear 384 are meshed with a fixed rack 385, and one end, close to the collecting frame 370, of the fixed rack 385 is connected with an L-shaped frame 386.

The lower end of the inclined rod 339 is abutted with an L-shaped rod 391, the L-shaped rod 391 penetrates through the horizontal plate 338 in a sliding manner, and the part of the L-shaped rod 391, which is positioned at the upper end of the horizontal plate 338, and the upper end of the horizontal plate 338 are connected with a supporting spring rod 392; in the initial state, the L-shaped rod 391 and the right-angle trapezoidal block 383 are placed in a non-contact staggered manner, when the right-angle trapezoidal block 383 is turned backwards through the turning block 390, the right-angle trapezoidal block 383 moves to the position right below the L-shaped rod 391, and when the L-shaped rod 391 moves along with the sealing plate 325 and synchronously moves downwards, the L-shaped rod 391 always abuts against the upper end of the right-angle trapezoidal block 383 to slide.

A magnetically attractable metal plate 387 is fixed at the lower end of the collecting frame 370, a magnetic sheet 388 is connected to the upper end of the L-shaped frame 386, and an electromagnetic coil to be electrified is arranged at the lower end of the magnetic sheet 388.

The inside of the L-shaped capturing frame 330 near the side wall of the sealing plate 325 is fixed with direction changing blocks 390 at equal intervals, and the direction changing blocks 390 are in a right-angled trapezoid structure.

The side wall of the L-shaped shooting frame 330 close to the sealing plate 325 is fixed with direction-changing blocks 390 at equal intervals, and the direction-changing blocks 390 are in a right-angled trapezoid structure; the side wall of the L-shaped shooting frame 330 close to the sealing plate 325 is fixed with direction-changing blocks 390 at equal intervals, and the direction-changing blocks 390 are in a right-angled trapezoid structure; when the second knob 360 is rotated, the two opening and closing belts 351 are synchronously moved, so that after the moving frame 352 is moved to a designated position, the right-angle trapezoidal block 383 is also moved to a designated place, and when the right-angle trapezoidal block 383 is moved downwards to a designated position, the right-angle trapezoidal block 383 can contact the direction changing block 390, and the position of the right-angle trapezoidal block 383 is changed after the right-angle trapezoidal block 383 contacts the direction changing block 390 until the right-angle trapezoidal block 383 abuts against the side wall of the direction changing block 390, and at the same time, the lower end of the right-angle trapezoidal block 383 is meshed with the gear 384.

When the inclined rod 339 is pressed downwards, the inclined rod 339 generates a certain pressure on the L-shaped rod 391 at the lower end thereof, so that the L-shaped rod 391 moves downwards, the L-shaped rod 391 moves downwards and presses the right-angled trapezoidal block 383, so that the right-angled trapezoidal block 383 moves away from the collecting frame 370, meanwhile, the L-shaped frame 386 at the lower end of the gear 384 approaches towards the collecting frame 370 through the action of the gear 384, and the L-shaped frame 386 is positioned right below the collecting frame 370, at the moment, the electromagnetic coil is electrified, so that a large magnetic force is generated between the magnetic sheet 388 and the metal plate 387, and the stability between the collecting frame 370 and the L-shaped frame 386 is further improved.

Then, the first knob 358 is rotated to retract the telescopic block 353 into the moving frame 352, at this time, the slant bar 339 loses the external force and returns to the initial state, and the L-shaped bar 391 also moves upward and returns under the supporting force of the supporting spring bar 392 until it is separated from the right-angled trapezoidal block 383, and the right-angled trapezoidal block 383 moves toward the sealing plate 325 after losing the pressure of the L-shaped bar 391 until it returns to the initial state, and at the same time, the collecting frame 370 slides out of the sliding groove, and finally, the second knob 360 is rotated in the reverse direction to move the collecting frame 370 upward to the uppermost end of the L-shaped capturing frame 330, and then, after the power is cut off, the collecting frame 370 is taken out from the horizontal portion of the L-shaped capturing frame 330.

The L-shaped ingestion frame 330 is in an inverted state, the horizontal portion thereof is hollow and sealed by a plug, and when it is necessary to remove the collection frame 370, it is opened, the collection frame 370 is removed, and finally the plug is covered again to seal it.

The implementation principle of the embodiment is as follows:

1: after adding the required auxiliary reagent of centrifugation in test tube A, the layering after will disinfecting and sterilizing absorbs mechanism 33 and inserts on the test tube A inner wall, then operating personnel places test tube A and passes fixed lantern ring 321, contradict test tube A the upper end of the inside bearing board 322 of control box after the disinfection, and it contradicts at the bar inslot to absorb mechanism 33 in the layering, after test tube A placed the inside of control box 1, contradict sealing plug B's the back on sucking disc 44, after the air discharge in sucking disc 44, sealing plug B then is fixed with sucking disc 44, sealing plug B is fixed at the lower extreme of sealing door 2 this moment, cover sealing door 2 in the upper end of control box 1 after, whole control box 1 is in inclosed state this moment.

2: after the inside of control box 1 was all placed to required sealed lid of test tube A and test tube A, sealing door 2 was covered to operating personnel, the preparation work before centrifugation was accomplished this moment, operating personnel rotated adjusting knob 462, adjusting knob 462 drives ring 460 through adjusting gear 461 and rotates, make the round hole coincidence on dislocation hole on the ring 460 and the sealing door 2, the biological sample after will handling passes through the round hole next, put into test tube A, reverse rotation adjusting knob 462 once more this moment, make ring 460 cover the upper end at the round hole again, make control box 1 be in inclosed state.

3: an operator rotates and presses the threaded rod 40, the sealing plug B is driven to move obliquely in the downward movement process of the trapezoidal column 41 until the sealing plug B is inserted into the opening of the test tube A, then the threaded rod 40 continues to rotate and press, the vent column 451 on the trapezoidal column 41 is inserted into the vent pipe 450, after the vent column 451 moves a certain distance in the vent pipe 450, the vent groove 452 on the vent column 451 is overlapped with the vent groove 452 on the vent pipe 450, the inside of the suction cup 44 is connected with the atmosphere through the vent groove 452 at the moment, air enters the position where the suction cup 44 is in contact with the sealing plug B, the suction cup 44 loses suction force, the sealing plug B covers the test tube A at the moment, the suction cup 44 is separated from the sealing plug B, and the sealing plug B cannot touch the suction cup 44 and the trapezoidal column 41 when the test tube A and the test tube B rotate centrifugally.

4: after the test tube a is sealed by the sealing plug B, the centrifugal motor 30 drives the disc 31 and the test tube a with the upper end fixed to rotate at a high speed until the test tube a is completely centrifuged.

5: after the biological sample in the test tube a is centrifuged, the centrifuged liquid is sequentially guided into the collection frame 370 through the L-shaped intake frame 330 from top to bottom to be collected and is sealed by the collection frame 370 to prevent it from contacting with the external air for a long time, and then the position of the collection frame 370 is adjusted and the collection frame 370 is taken out.

The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.

Claims

1. The utility model provides a biological gene tissue sample centrifugation sampling system, includes control box (1) and control box (1) upper end left side articulated sealing door (2), its characterized in that: a centrifugal part (3) for performing centrifugal operation on a test tube for placing biological genetic tissues is arranged in the control box (1), and a sucker fixer (4) for fixing a test tube plug is arranged on the inner side of the sealing door (2);

the centrifugal part (3) comprises a centrifugal motor (30) fixed at the bottom end inside the control box (1), a disc (31) is installed at the upper end of the centrifugal motor (30) and used for placing a test tube of biological gene tissues, a placing part (32) used for fixing the test tube is arranged at the upper end of the disc (31), and a layered taking mechanism (33) used for centrifugally sampling samples in the test tube is arranged on the placing part (32).

2. A centrifugal biological gene tissue sample sampling system according to claim 1, wherein: place portion (32) including disc (31) upper end along its circumference direction equidistant articulated four groups arc (320) that are used for placing the test tube, the arc recess has been seted up on the lateral wall of arc (320), the bar groove has been seted up in the arc recess, be used for placing the test tube, one side that arc (320) are close to the arc recess is fixed with carries out spacing fixed lantern ring (321) to the test tube, the bottom in arc (320) outside is equipped with and carries out bearing board (322) of bearing to the test tube, the inside wall of arc (320) is equipped with regulating part (34).

3. A biological gene tissue sample centrifugal sampling system according to claim 2, characterized in that: the adjusting part (34) comprises a central column (340) fixed in the middle of the upper end of the disc (31), the central column (340) slides up and down and is sleeved with an adjusting lantern ring (341), the outer side wall of the adjusting lantern ring (341) is hinged with an adjusting rod (342) at equal intervals along the circumferential direction of the outer side wall, one end, far away from the adjusting lantern ring (341), of the adjusting rod (342) is hinged with the arc-shaped plate (320), the lower end of the adjusting lantern ring (341) is connected with an electric push rod (343), and the electric push rod (343) is fixed at the upper end of the disc (31).

4. A centrifugal biological gene tissue sample sampling system according to claim 1, wherein: sucking disc fixer (4) are including sealing door (2) upper end through threaded connection's the threaded rod of pressing (40), the lower extreme of pressing threaded rod (40) is rotated through the bearing and is connected with trapezoidal post (41), the upper end of trapezoidal post (41) is connected with spacing flexible post (42) with the lower extreme of sealing door (2), the equidistant telescopic spring pole (43) that is fixed with along the circumferential direction in its axle center on the inclined plane lateral wall of trapezoidal post (41), the one end fixedly connected with that trapezoidal post (41) were kept away from in telescopic spring pole (43) is used for sucking disc sealing plug (44) of fixed test tube sealing plug, be equipped with on sucking disc (44) and initiatively loosen executive (45) of test tube.

5. A centrifugal sampling system for a biological gene tissue sample according to claim 4, wherein: the actuating member (45) runs through fixed breather pipe (450) including sucking disc (44) near the one end of telescopic spring pole (43), there is ventilation post (451) through sealing washer sliding connection in breather pipe (450), vent groove (452) have been seted up to one side that breather pipe (450) were kept away from to ventilation post (451) lower extreme, vent groove (452) the same on one side that breather pipe (450) lower extreme is close to sucking disc (44) have been seted up with ventilation post (451), vent post (451) are fixed on the lateral wall of trapezoidal post (41).

6. A centrifugal biological gene tissue sample sampling system according to claim 1, wherein: the layered ingestion mechanism (33) comprises an L-shaped ingestion frame (330) movably arranged on the side wall of the test tube, the front side wall and the rear side wall of the L-shaped ingestion frame (330) are fixedly connected with a first plate (331), the left side of the first plate (331) is fixedly connected with a clamping plate (333) through a clamping spring rod (332), one end, far away from the clamping plate (333), of the L-shaped ingestion frame (330) is provided with a plurality of liquid extraction holes distributed from top to bottom at equal intervals, a liquid outlet pipe (334) is fixed in each liquid extraction hole, the lower side inside the liquid outlet pipe (334) is provided with a chute (335), a sealing column (336) slides left and right in the liquid outlet pipe (334), one end, far away from the liquid outlet pipe (334), of the sealing column (336) is connected with a sealing plate (325), one side, far away from the sealing column (336), of the sealing plate (325) is connected with the inner wall of the L-shaped ingestion frame (330) through an extrusion spring rod (337), and an opening and closing module (35) for opening and closing the liquid extraction holes is arranged on the L-closing frame (330);

the upper end of the sealing plate (325) is fixed with a horizontal plate (338), and the horizontal plate (338) is hinged with two symmetrically distributed sway rods (339).

7. A centrifugal biological gene tissue sample sampling system according to claim 6, wherein: the opening and closing module (35) comprises rotating shafts (350) rotating at the upper end and the lower end of the inner wall of an L-shaped shooting frame (330), two opening and closing belts (351) which are symmetrical front and back are jointly rotated on the two rotating shafts (350), a movable frame (352) is fixed on the side wall of the opening and closing belt (351) positioned on the rear side in the L-shaped shooting frame (330) through a pin shaft, one end, far away from the closing belt (351), of the movable frame (352) penetrates through a telescopic block (353) in a sliding mode, one side, located inside the movable frame (352), of the telescopic block (353) is abutted to a triangular block (354), an execution spring (357) is connected between the telescopic block (353) and the inner wall of the movable frame (352), the triangular block (354) slides up and down in a limiting groove in the inner wall of the movable frame (352), a twisted rope (355) is connected to the upper end of the triangular block (354), a reset spring (356) is sleeved between the triangular block (354) and the inner wall of the movable frame (352), and the return spring (356) is sleeved on the stranded rope (355);

a first knob (358) is rotatably arranged at the upper end of the L-shaped ingestion frame (330), a winding threaded rod (359) penetrates through the lower end of the first knob (358) through a bearing in a rotating manner, the winding threaded rod (359) is connected with a stranded rope (355), and the stranded rope (355) is wound on the winding threaded rod (359);

a second knob (360) is rotatably arranged on the outer side wall of the L-shaped ingestion frame (330), and the side wall of the second knob (360) is connected with the rotating shaft (350) through a bearing;

a sample collecting component (37) is arranged on the opening and closing belt (351) positioned at the front side inside the L-shaped ingestion frame (330).

8. A centrifugal biological gene tissue sample sampling system according to claim 1, wherein: sample collection subassembly (37) include that L shape is absorb on frame (330) inner wall and be located the spout that sets up under closing plate (325), slidable mounting has collection frame (370) in the spout, the upper end fixed mounting of collection frame (370) has apron (371) No. one, the flexible groove has been seted up on the lateral wall of apron (371), there is apron (373) No. two through sealing spring (372) slidable mounting in the flexible groove, the one side of apron (371) is kept away from to the upper end of apron (373) No. two is fixed with passive piece (374), U-shaped frame (375) is installed to the lower extreme of closing plate (325), the middle part of U-shaped frame (375) is slided in around the fixed passive piece (374) in apron (373) upper end No. two.

9. A centrifugal biological gene tissue sample sampling system according to claim 6, wherein: a vertical plate (380) is fixed on the side wall of the opening and closing belt (351) positioned on the front side in the L-shaped ingestion frame (330), the side end of the vertical plate (380) is connected with a butting spring (381), one side, far away from the vertical plate (380), of the butting spring (381) is fixedly connected with a T-shaped frame (382), a right-angle trapezoidal block (383) provided with a T-shaped groove is connected onto the T-shaped frame (382) in a sliding mode, the inclined surface of the right-angle trapezoidal block (383) is distributed towards the direction of the sealing plate (325), the lower end of the right-angle trapezoidal block (383) is of a rack structure, a gear (384) is meshed at the lower end of the right-angle trapezoidal block (383), a fixed rack (385) is meshed at the lower end of the gear (384) close to the collection frame (370), and an L-shaped frame (386) is connected at one end of the fixed rack (385);

a metal plate (387) capable of being magnetically attracted is fixed at the lower end of the collecting frame (370), a magnetic sheet (388) is connected at the upper end of the L-shaped frame (386), and an electromagnetic coil to be electrified is arranged at the lower end of the magnetic sheet (388);

the side wall of the interior of the L-shaped shooting frame (330) close to the sealing plate (325) is fixed with direction-changing blocks (390) at equal intervals, and the direction-changing blocks (390) are of a right-angle trapezoidal structure;

the lower end of the inclined rod (339) is abutted with an L-shaped rod (391), the L-shaped rod (391) penetrates through the horizontal plate (338) in a sliding mode, and the part, located at the upper end of the horizontal plate (338), of the L-shaped rod (391) and the upper end of the horizontal plate (338) are connected with a supporting spring rod (392).

10. A centrifugal biological gene tissue sample sampling system according to claim 1, wherein: four round holes have been seted up to the position that the upper end of sealing door (2) corresponds the test tube, the inside of sealing door (2) is hollow structure, and the inboard rotation of sealing door (2) has ring (460), set up the dislocation hole that corresponds with four round holes on ring (460), the meshing has adjusting gear (461) on the lateral wall of ring (460), the upper end of adjusting gear (461) is connected with adjust knob (462) through center pin (463), adjust knob (462) rotate the upper end at sealing door (2).