CN115040683A - Composite nanofiber membrane dressing with high oxidation resistance and antibacterial property - Google Patents
Composite nanofiber membrane dressing with high oxidation resistance and antibacterial property Download PDFInfo
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- CN115040683A CN115040683A CN202210774850.1A CN202210774850A CN115040683A CN 115040683 A CN115040683 A CN 115040683A CN 202210774850 A CN202210774850 A CN 202210774850A CN 115040683 A CN115040683 A CN 115040683A
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- Prior art keywords
- nanofiber membrane
- composite nanofiber
- membrane
- dressing
- sodium alginate
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- 239000012528 membrane Substances 0.000 title claims abstract description 80
- 239000002121 nanofiber Substances 0.000 title claims abstract description 71
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 11
- 230000003647 oxidation Effects 0.000 title abstract description 7
- 238000007254 oxidation reaction Methods 0.000 title abstract description 7
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 34
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000000661 sodium alginate Substances 0.000 claims abstract description 29
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 29
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 29
- KFFCKOBAHMGTMW-LGQRSHAYSA-N Forsythin Chemical compound C1=C(OC)C(OC)=CC=C1[C@H]1[C@@H](CO[C@@H]2C=3C=C(OC)C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC=3)[C@@H]2CO1 KFFCKOBAHMGTMW-LGQRSHAYSA-N 0.000 claims abstract description 17
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 10
- JJVGFDTWFVSBIM-UHFFFAOYSA-N Phillyrin Natural products COc1ccc(cc1OC)C2OCC3C2COC3c4ccc(OC)c(OC5OC(CO)C(O)C(O)C5O)c4 JJVGFDTWFVSBIM-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004132 cross linking Methods 0.000 claims abstract description 10
- 238000009987 spinning Methods 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 27
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 15
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000000835 fiber Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 229920001046 Nanocellulose Polymers 0.000 claims description 2
- 239000012567 medical material Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000004094 surface-active agent Substances 0.000 claims description 2
- 230000035876 healing Effects 0.000 abstract description 6
- 210000000416 exudates and transudate Anatomy 0.000 abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 208000027418 Wounds and injury Diseases 0.000 description 17
- 206010052428 Wound Diseases 0.000 description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 230000029663 wound healing Effects 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 238000001027 hydrothermal synthesis Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- -1 nano silver ions Chemical class 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
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- D06M11/11—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with halogens; with halogen acids or salts thereof; with oxides or oxyacids of halogens or salts thereof with halogen acids or salts thereof
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Abstract
The invention provides a composite nanofiber membrane dressing with high oxidation resistance and antibacterial property, which is characterized in that phillyrin is added into a sodium alginate solution, and then a nanofiber membrane is obtained through electrostatic spinning; and then, crosslinking by adopting 2 wt% of calcium chloride solution to obtain the composite nanofiber membrane dressing. According to the invention, the carbon dots are utilized to improve the sodium alginate nanofiber membrane, so that the high-concentration sodium alginate electrostatic spinning membrane can be prepared, has high hygroscopicity, and also has high biocompatibility, and then the fructus forsythiae is loaded on the nanofiber membrane, so that the nanofiber membrane is endowed with high oxidation resistance and high hygroscopicity, the excessive active oxygen of a wound is reduced, and the excessive wound exudate is absorbed. Meanwhile, the nanofiber membrane has better antibacterial performance. Can greatly accelerate the healing speed of the wound.
Description
Technical Field
The invention belongs to the technical field of biomedical articles, and particularly relates to a composite nanofiber membrane dressing with high oxidation resistance and antibacterial property.
Background
Wounds are one of the common ailments in daily life, and the adhesion of wound exudates and common dressings can delay the healing of wounds and cause secondary injury. Many of the existing biological dressings use nano silver ions as an antimicrobial agent, which may cause some body discomfort. Currently, the use of biological dressings with natural antimicrobial agents to promote wound healing has become a major trend.
The nanofiber membrane dressing studied in the past has single performance and cannot adapt to complex wound conditions. Therefore, it is of great significance to develop a multifunctional nanofiber membrane as a dressing.
Disclosure of Invention
The invention aims to provide a composite nanofiber membrane dressing with high oxidation resistance and antibacterial property, thereby making up the defects of the prior art.
The invention firstly provides a composite nanofiber membrane dressing, which is characterized in that phillyrin is added into a sodium alginate solution, and then a nanofiber membrane is obtained through electrostatic spinning; and then, crosslinking by adopting 2 wt% of calcium chloride solution to obtain the composite nanofiber membrane dressing.
Furthermore, carbon dots are also added into the sodium alginate solution;
preferably, the concentration of the sodium alginate solution is 92%.
The composite nanofiber membrane dressing has a specific preparation method as follows:
1) mixing a sodium alginate aqueous solution and a polyethylene oxide aqueous solution, adding a surfactant, dimethyl sulfoxide, 0.5 wt% of phillyrin and 0.2 wt% of carbon dots, and stirring at normal temperature to obtain a spinning solution;
2) preparing the spinning solution into a nano cellulose membrane by an electrostatic spinning method;
3) soaking the nanofiber membrane in absolute ethyl alcohol, then crosslinking in 2 wt% calcium chloride solution for 10s, and washing with deionized water to obtain the composite nanofiber membrane dressing.
The composite nanofiber membrane dressing provided by the invention is applied as a medical material.
According to the invention, the carbon dots are utilized to improve the sodium alginate nanofiber membrane, so that the high-concentration sodium alginate electrostatic spinning membrane can be prepared, has high hygroscopicity, and also has high biocompatibility, and then the fructus forsythiae is loaded on the nanofiber membrane, so that the nanofiber membrane is endowed with high oxidation resistance and high hygroscopicity, the excessive active oxygen of a wound is reduced, and the excessive wound exudate is absorbed. Meanwhile, the nanofiber membrane has better antibacterial performance. Can greatly accelerate the healing speed of the wound.
Drawings
Fig. 1 is a scanning electron microscope image of a sodium alginate nanofiber membrane in example 1 of the present invention, wherein (a) is the sodium alginate nanofiber in example 1, (b) is the sodium alginate-coated carbon dot nanofiber in example 2, and (c) is the carbon dot sodium alginate-loaded forsythin nanofiber in example 3.
Fig. 2 is a moisture absorption diagram of gauze, a band-aid and three nanofiber membranes prepared by the example.
Fig. 3 is a graph of the antioxidant performance of three nanofiber membranes prepared in the examples at different times.
Fig. 4 is a graph of the antibacterial effect of the forsythin-loaded nanofibers.
FIG. 5 is a cytocompatibility plot of three nanofiber membranes.
Figure 6 is a picture of a mouse wound over 0-14 days for petrolatum gauze and three nanofiber membranes.
Detailed Description
In research, the applicant finds that the forsythin is introduced into the sodium alginate spinning solution, so that the nano fiber membrane can be endowed with antibacterial and antioxidant properties. However, the physicochemical properties of the prepared nanofiber membrane are not ideal when phillyrin is directly added into the 92% sodium alginate spinning solution. After the carbon dot (carbon quantum dot) component is added, the property of the fiber membrane prepared by high-concentration sodium alginate electrostatic spinning can be improved, and meanwhile, the cell compatibility and the hygroscopicity of the nanofiber membrane are also improved.
The invention discovers that the carbon dots with the addition amount of 0-0.4 wt% can improve the spinning condition of the nanofiber membrane and improve the hygroscopicity and the mechanical property of the nanofiber membrane to the spinning condition of the sodium alginate spinning solution with the mass fraction of 92%, and then 0.2 wt% of the carbon dots can be added into the spinning solution to prepare the nanofiber membrane with good fiber morphology, and then 2 wt% of calcium chloride solution is adopted for crosslinking to obtain the water-insoluble fiber membrane dressing.
The present invention will be described in detail below with reference to examples and the accompanying drawings.
Example 1
1) Preparing a spinning solution: taking sodium alginate and polyethylene oxide (PEO) according to a mass ratio of 9.2: 0.8, adding into a small beaker to prepare a sodium alginate spinning solution with the concentration of 92 percent, adding 1.5 weight percent of TX-100 and 20 weight percent of dimethyl sulfoxide, and stirring on a magnetic stirrer for 5-6 hours.
2) The sodium alginate nanofiber membrane is obtained by spinning the spinning solution through electrostatic spinning, and the method comprises the following specific steps:
the first step is to regulate the spinning condition, the temperature is 30-35 ℃, and the humidity is below 30%.
And secondly, after the external regulation reaches the spinning condition, a section of 30cm of tinfoil is stuck on a receiver roller.
And thirdly, taking a 10ml needle tube to suck 6-7ml of spinning solution, connecting a 21G needle head to the other end of the needle tube, placing the needle tube in a mode injection groove, and then pushing an injection pump by hand until liquid overflows from the needle point.
And fourthly, adjusting the propelling speed to be 0.5ml/h, the distance between the receiver and the needle point to be 18cm, and the rotating speed of the roller to be 60 rad/min.
And fifthly, clamping a positive voltage clamp of voltage on the needle tip, and adjusting the voltage to be 16 kv.
And sixthly, carrying out electrostatic spinning for 5-6 hours, then regulating the voltage to 0, stopping spinning, and taking down the SA film on the tinfoil.
3) Soaking the nanofiber membrane in absolute ethyl alcohol for 1min, then crosslinking in 2 wt% of calcium chloride for 10s, and washing with deionized water to obtain the water-insoluble SA membrane.
4) The crosslinked sample was dried in a vacuum oven.
Example 2
1) Preparing a spinning solution: taking sodium alginate and polyethylene oxide, wherein the mass ratio of sodium alginate to polyethylene oxide is 9.2: 0.8, then adding 1.5 wt% of TX-100, 20 wt% of dimethyl sulfoxide and 0.2 wt% of carbon dots, and stirring on a magnetic stirrer for 5-6 hours.
The Carbon Dots (CDs) used were prepared by a hydrothermal reaction, 1.0507g of citric acid and 335. mu.l of ethylenediamine were dissolved in 10ml of deionized water, and then the solution was introduced into a reaction vessel, placed in an oven at 200 ℃ for reaction for 5 hours, and after the reaction vessel was cooled, the solution was taken out and lyophilized in a lyophilizer to obtain carbon dot powder.
2) The spinning solution is subjected to electrostatic spinning to obtain the carbon dot loaded nanofiber membrane, and the specific steps are as follows:
the first step is to regulate the spinning condition, the temperature is 30-35 ℃, and the humidity is below 30%.
And secondly, after the external regulation reaches the spinning condition, a section of 30cm of tinfoil is stuck on a receiver roller.
And thirdly, taking a 10ml needle tube to absorb 6-7ml of spinning solution, connecting a 21G needle at the other end, placing the needle in a mode injection groove, and then pushing an injection pump by hand until the needle point overflows with liquid.
And fourthly, adjusting the propelling speed to be 0.5ml/h, the distance between the receiver and the needle point to be 18cm, and the rotating speed of the roller to be 60 rad/min.
And a fifth step of clamping a positive voltage clamp of the voltage on the needle tip, and adjusting the voltage to be 18 kv.
And sixthly, carrying out electrostatic spinning for 5-6 hours, then regulating the voltage to 0, stopping spinning, and taking down the SA/PEO/CDs film on the tin foil.
3) Soaking the nanofiber membrane in absolute ethyl alcohol for 1min, then crosslinking in 2 wt% calcium chloride for 10s, and washing with deionized water to obtain the water-insoluble SA/PEO/CDs membrane.
4) The crosslinked sample was dried in a vacuum oven.
Example 3
1) Preparing a spinning solution: taking sodium alginate and polyethylene oxide, wherein the mass ratio of sodium alginate to polyethylene oxide is 9.2: 0.8, then adding 1.5 wt% of TX-100, 20 wt% of dimethyl sulfoxide, 0.2 wt% of carbon dots and 0.5 wt% of phillyrin, and stirring for 5-6 hours on a magnetic stirrer.
2) The forsythin-loaded carbon dot nanofiber membrane is obtained by performing electrostatic spinning on the spinning solution, and the steps are as follows:
firstly, adjusting spinning conditions, wherein the temperature is 30-35 ℃, and the humidity is below 30%.
And secondly, after the external regulation reaches the spinning condition, a section of 30cm of tinfoil is stuck on a receiver roller.
And thirdly, taking a 10ml needle tube to absorb 6-7ml of spinning solution, connecting a 21G needle at the other end, placing the needle in a mode injection groove, and then pushing an injection pump by hand until the needle point overflows with the liquid.
And fourthly, adjusting the propelling speed to be 0.5ml/h, the distance between the receiver and the needle point to be 18cm, and the rotating speed of the roller to be 60 rad/min.
And fifthly, clamping a positive voltage clamp of voltage on the needle tip, and adjusting the voltage to be 18 kv.
And sixthly, carrying out electrostatic spinning for 5-6 hours, then regulating the voltage to 0, stopping spinning, and taking down the FT-SA/CDs film on the tin foil.
3) Soaking the nanofiber membrane in absolute ethyl alcohol for 1min, then crosslinking in 2 wt% of calcium chloride for 10s, and washing with deionized water to obtain the water-insoluble FT-SA/CDs membrane.
4) The crosslinked sample was dried in a vacuum oven.
Example 4
The results of analyzing the appearance of the nanofiber membranes prepared in examples 1 to 3 by using a scanning electron microscope are shown in fig. 1, wherein a) is the sodium alginate nanofiber membrane prepared in example 1, b) is the carbon dot nanofiber membrane prepared in example 2, and c) is the forsythin-loaded carbon dot nanofiber membrane prepared in example 3. It can be seen that many beads appear on the fiber membrane in fig. 1a, resulting in defects in the fiber morphology, whereas fig. 1b and 1c show perfect fiber morphology without defects of beads, droplets, etc. The result shows that the carbon points are introduced to improve the morphology of the nanofiber membrane, and the phillyrin is added to still maintain good fiber morphology.
Example 5
The moisture absorption of the woundplast, gauze, the nanofiber membranes of example 2 and example 3 were tested by the water absorption performance. FIG. 2 is a graph comparing the moisture absorption of the bandage, gauze, SA/PEO/CDs film and FT-SA/CDs film, and the results show that the moisture absorption of the bandage and the gauze is 82.8% and 118.18%, respectively, whereas the moisture absorption of the FT-SA/CDs film is almost 6-8 times that of the gauze and the bandage. The results show that the forsythin-loaded nanofiber membrane has the best hygroscopicity.
Example 6
The nanofiber membranes of examples 1-3 were tested by DPPH free radical scavenging experiments and analyzed using an ultraviolet spectrophotometer. FIG. 3 is a graph of the reaction time versus radical scavenging rate for the SA, SA/PEO/CDs and FT-SA/CDs films of examples 1-3, and it was found that the SA and SA/PEO/CDs scavenge radicals in solution slowly, and the DPPH radical scavenging rate slightly increases with time, whereas the FT-SA/CDs film achieved a good effect within one hour from the start. The result shows that the forsythin-loaded nanofiber membrane has high-efficiency antioxidant performance.
Example 7
The FT-SA/CDs membrane of example 3 was tested for Staphylococcus aureus and Escherichia coli by plate coating, and the antibacterial effect was analyzed by counting the number of colonies. The results are shown in FIG. 4, and compared with the blank group, the colony number of the FT-SA/CDs membrane experimental group is obviously reduced; the results show that the nano-fiber membrane containing the phillyrin has the antibacterial effect.
Example 8
The biocompatibility of the nanofiber membranes of examples 1 to 3 was analyzed by the MTT method, and the results are shown in fig. 5. FIG. 5 is a graph showing the biocompatibility effects of the SA membrane, the SA/PEO/CDs membrane and the FT-SA/CDs membrane in the blank control group, examples 1 to 3, three membranes were co-cultured with mouse fibroblasts for 24h and 48h, and no reduction of cells was observed in all three fiber membranes compared with the blank. The results show that the three nanofiber membranes have good biocompatibility.
Example 9
The wound healing in vitro of regular gauze and nanofiber membranes of examples 1-3 was analyzed by in vitro mouse experiments. The results are shown in FIG. 6, which is a graph of the effect of the in vitro wound healing test on mice divided into gauze, SA films in examples 1 to 3, SA/PEO/CDs films and FT-SA/CDs films from top to bottom as a function of time. Observing the control sample (gauze group), wherein the initial wounds are all circles of 1cm, and on the fourth day of wound healing, the sizes of four groups of wounds are not greatly different, and the healing speed is not greatly different, when the wounds heal for eight days, the FT-SA/CDs membrane group wounds are found to scab, and the healing speed is slightly high, after the twelfth day, the FT-SA/CDs membrane group wounds can be obviously seen to be healed, while the other three groups are not completely healed, particularly the gauze group has the largest wound area and low healing speed, and after the sixteenth day, the wounds heal better and the wounds are basically completely healed except the gauze group. The results indicate that the FT-SA/CDs film has good ability to promote wound healing.
Claims (9)
1. The composite nanofiber membrane dressing is characterized in that phillyrin is added into a sodium alginate solution, and then a fiber membrane is obtained through electrostatic spinning; and then, crosslinking by adopting a calcium chloride solution to obtain the composite nanofiber membrane dressing.
2. The composite nanofiber membrane dressing as claimed in claim 1, wherein carbon dots are further added to the sodium alginate solution.
3. The composite nanofiber membrane dressing of claim 1 or 2, wherein the concentration of the sodium alginate solution is 92%.
4. The composite nanofiber film dressing of claim 1, wherein the composite nanofiber film dressing is prepared by the following method:
1) mixing a sodium alginate aqueous solution and a polyethylene oxide aqueous solution, adding a surfactant, dimethyl sulfoxide, phillyrin and carbon dots, and stirring at normal temperature to obtain a spinning solution;
2) preparing the spinning solution into a nano cellulose membrane by an electrostatic spinning method;
3) soaking the nanofiber membrane in absolute ethyl alcohol, then crosslinking in a calcium chloride solution, and washing with deionized water to obtain the composite nanofiber membrane dressing.
5. The composite nanofiber film dressing as claimed in claim 4, wherein the concentration of phillyrin added is 0.5%.
6. The composite nanofiber film dressing of claim 4, wherein the carbon dots are added at a concentration of 0.2%.
7. The composite nanofiber film dressing of claim 4, wherein the concentration of the calcium chloride solution is 2%.
8. The composite nanofiber film dressing of claim 4, wherein the crosslinking time is 10 s.
9. Use of the composite nanofiber film dressing of claim 1 as a medical material.
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