CN115039779B - Application of dimethyl trisulfide in inhibition of downy mildew of litchi - Google Patents

Application of dimethyl trisulfide in inhibition of downy mildew of litchi Download PDF

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CN115039779B
CN115039779B CN202210776875.5A CN202210776875A CN115039779B CN 115039779 B CN115039779 B CN 115039779B CN 202210776875 A CN202210776875 A CN 202210776875A CN 115039779 B CN115039779 B CN 115039779B
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dimethyl trisulfide
litchi
pathogenic bacteria
bacteria
colletotrichum
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CN115039779A (en
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唐利华
李其利
郭堂勋
黄穗萍
莫贱友
陈小林
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to application of dimethyl trisulfide in inhibiting downy mildew. According to the invention, the indoor toxicity measurement proves that the dimethyl trisulfide has different degrees of inhibition effects on different pathogenic bacteria, wherein the inhibition effects on corn rhizoctonia solani and grape gray mold bacteria are the best, and the second is the dragon fruit canker and rice blast bacteria, so that the dimethyl trisulfide can be used as a potential biocontrol agent for plant pathogenic bacteria.

Description

Application of dimethyl trisulfide in inhibition of downy mildew of litchi
The application is a divisional application with the application number of 2021110875692 and the invention name of dimethyl trisulfide for inhibiting the application of downy mildew.
Technical Field
The invention belongs to the field of plant protection, and particularly relates to application of dimethyl trisulfide in inhibiting downy mildew.
Background
Dimethyl trisulfide (DMTS) (also known as dimethyl trisulfide) is a volatile sulfur compound that is found in leeks, onions and other allium species, broccoli, cabbage, ringer's cheese, japanese sake, and some volatile substances released by bacteria and fungi.
Mango anthracnose is a serious disease on mango, and occurs in mango producing areas around the world, and pathogenic bacteria mainly comprise colletotrichum gloeosporioides (Colletotrichum gloeosprioides) complex groups, and Asian anthracnose (Colletotrichum asianum), fruit anthracnose (Colletotrichum fructicola) and Siamese anthracnose (Colletotrichum siamense) are 3 dominant species. The disease mainly damages mango leaves, tender tips, inflorescences and fruits, and can cause plant tip withered, leaf withered, flower and fruit falling and mass decay of picked fruits in a mango park. The black spots appear in the fruits at the initial stage of the disease, the spots are enlarged to form black round, oval or irregular spots, the spots are often sunken, red spore piles are often generated at the spots under the moist environment, and finally the whole fruits turn black and rot. The pathogenic bacteria have wide host range, endanger various fruits and vegetables, trees, flowers and the like besides mango, and most tropical and subtropical fruit trees are also important hosts.
The rice blast is one of important diseases in global rice production, has a great threat to the yield and quality of rice, can cause the total paddy field to be insulated when serious occurrence occurs, and the rice blast bacteria (Magnaporthe oryzae) spread through conidium, germinate on the surface of rice leaves to generate bud tubes and form attachment cells, then generate infection plugs to penetrate through horny layers and epidermis cell walls of the leaves, generate secondary hyphae in host cells and infect adjacent cells and tissues, and then attack the rice.
The orange peel of the granulated sugar is thin, is easy to be mechanically damaged in the processes of harvesting, storing and transporting, is easy to be infected by pathogenic bacteria to cause decay, and has a short storage period after harvesting. Among them, the green mold caused by pathogenic spores of Penicillium digitatum (Penicillium digitatum) is one of the main causes of decay after the harvest of the sugar orange.
Fusarium oxysporum (Fusarium oxysporum f.sp.cube) is an earth-borne pathogenic fungus widely distributed worldwide, has a wide pathogenic area and serious pathogenicity, and is listed as one of ten plant pathogenic fungi in the world. The fusarium oxysporum can infect more than 100 crops with important value to cause wilt and root rot, such as tomatoes, bananas, strawberries, watermelons and the like, and the fusarium oxysporum infects plants from the roots, can cause plant morbidity in the whole growth period of the plants, mainly shows that brown necrotic spots are generated on root cortex of the plants, main roots and a large number of lateral roots are rotted when serious, and branches are reduced to death of the plants; the vascular tissue at the root of the plant is browned, and the overground part is withered until withered, so that the growth, the yield and the quality of the plant are seriously affected.
The rot of picked fruits caused by banana anthracnose is one of the most important fungal diseases in banana production, and 3 dominant pathogenic bacteria of the rot are fruit anthracnose (Colletotrichum fructicola), clivia anthracnose (Colletotrichum cliviicola) and Siamese anthracnose (Colletotrichum siamense) respectively. Banana anthracnose mainly damages mature banana fruits, brown or black brown small round spots appear on stalks and peel at first, then the spots rapidly spread and fuse with each other, the peel often turns black brown within 2-3 days, pulp decays, and the fruit value is seriously reduced.
The sheath blight of corn is a main disease of corn main production areas around the world, pathogenic bacteria are rhizoctonia solani (Rhizoctonia solani), the sheath blight of corn is mainly the leaf sheath part of harmful corn, and the sheath blight of corn is a serious disease which is a serious disease affecting the corn yield, and has great loss to agricultural production.
The downy mildew of litchi is caused by downy mildew of litchi (Peronophythora litchii), can cause browning and rot of litchi fruits, causes main diseases of yield reduction and yield instability, and commonly occurs in litchi cultivation areas in China; the disease is the damage of young shoots, young leaves and spikes of litchi, and can cause the economic loss of litchi industry to be more than 80% in the year of proper onset of climatic conditions, thereby severely limiting the development of litchi industry.
The dragon fruit canker is one of the most serious diseases in the production and management of the dragon fruit at present, and the pathogenic bacteria are new and darkly-colored aschersonia (Neoscytalidium dimidiatum). The disease mainly damages the stem of the dragon fruit, causes stem rot and fruit cracking when serious, even causes pulp brown rot or black rot, and has certain influence on the production of the dragon fruit.
Grape anthracnose is mainly caused by infection of colletotrichum gloeosporioides (Colletotrichum gloeosporioides), mainly damages grape fruits, and has obvious symptoms in near-maturity or mature period. After the fruit is damaged, firstly, a brown small circular spot with the size of a needle head is generated on the fruit surface, then the brown small circular spot is gradually enlarged and sunken, dark black small particles which are arranged in a concentric ring shape are generated on the surface, namely, conidiophores of pathogenic bacteria appear on the disease position when the environmental humidity is high, and when the disease is serious, the spot is expanded to the whole spike, and the disease spike rate is 50% -70%, so that the damage to the grape industry is serious.
Botrytis cinerea is a common fruit and vegetable disease caused by Botrytis cinerea infection, and is also one of the most harmful plant diseases. Besides damaging tomatoes, the botrytis cinerea can also damage a plurality of important commercial crops such as eggplants, peppers, cucumbers, grapes, strawberries and the like, and can cause gray mold of fruits and vegetables. The disease may occur not only during the season in which the host plant is growing, but also during the storage of agricultural products.
Disclosure of Invention
In view of the above-mentioned requirements, the present invention provides the use of dimethyl trisulfide for inhibiting plant pathogenic bacteria. Indoor toxicity measurement proves that the dimethyl trisulfide has obvious inhibition effect on plant pathogenic bacteria. Dimethyl trisulfide is a potential biocontrol inhibitor, and is efficient and low in toxicity.
Application of dimethyl trisulfide in inhibiting plant pathogenic bacteria including Asian anthracnose (Colletotrichum asianum), fruit anthracnose (Colletotrichum fructicola), row anthracnose (Colletotrichum siamense), clivia nobilis anthracnose (Colletotrichum cliviicola), botrytis cinerea (Colletotrichum gloeosporioides), pyricularia oryzae (Magnaporthe oryzae), rhizoctonia citri (Penicillium digitatum), rhizoctonia zeae (Rhizoctonia solani), phytophthora litchi (Phytophthora litchii), pitaya ulcer bacteria (Neoscytalidium dimidiatum) and Botrytis cinerea (Botrytis cinerea).
The host of the plant pathogenic bacteria is mango, sugar orange, banana, litchi, dragon fruit, grape, rice or corn.
The plant pathogenic bacteria are rice blast fungus (Magnaporthe oryzae), corn Rhizoctonia solani (Rhizoctonia solani), pitaya canker fungus (Neoscytalidium dimidiatum) and Botrytis cinerea, and the hosts are rice, corn, pitaya and grape respectively.
The application is to fumigate plant pathogenic bacteria by using dimethyl trisulfide.
In the fumigation treatment, the concentration gradient of the dimethyl trisulfide is 0.1 mu L/L-40 mu L/L.
In the fumigation treatment, the concentration gradient of the dimethyl trisulfide is 0.1-20 mu L/L.
The plant pathogenic bacteria are rice blast fungus (Magnaporthe oryzae), corn Rhizoctonia solani (Rhizoctonia solani), dragon fruit canker fungus (Neoscytalidium dimidiatum) and Botrytis cinerea (Botrytis cinerea), and the concentration gradient of dimethyl trisulfide is 0.1 mu L/L-10 mu L/L.
The invention has the beneficial effects that: the method has the advantages that the dimethyl trisulfide is utilized to carry out fumigation treatment on pathogenic bacteria in a closed environment, so that the antibacterial effect is achieved, and the fact that the dimethyl trisulfide has different degrees of inhibition effects on different pathogenic bacteria is determined through indoor toxicity measurement, wherein the inhibition effects on rhizoctonia solani and botrytis cinerea are best, and the second is dragon fruit canker and rice blast fungus, so that the dimethyl trisulfide can be used as a potential biocontrol preparation for plant pathogenic bacteria.
Drawings
FIG. 1 shows colony morphology of (mango) fruit anthracnose (Colletotrichum fructicola), (mango) Asian anthracnose (Colletotrichum asianum), (mango) siamese anthracnose (Colletotrichum siamense) and Pyricularia oryzae (Magnaporthe oryzae) treated with dimethyl trisulfide;
FIG. 2 shows colony morphology of Fusarium citri (Penicillium digitatum), (banana) Fusarium oxysporum (Fusarium oxysporum f.sp.cube), (banana) fruit anthrax (Colletotrichum fructicola), and (Colletotrichum cliviicola) clivia treated with dimethyl trisulfide;
FIG. 3 shows colony morphology of Siamese anthracis (Colletotrichum siamense), rhizoctonia solani (Rhizoctonia solani), phytophthora litchi (Phytophthora litchii) and Botrytis cinerea (Colletotrichum gloeosporioides) treated with dimethyl trisulfide;
FIG. 4 shows colony morphology of Botrytis cinerea and Pitaya ulcer bacteria (Neoscytalidium dimidiatum) treated with dimethyl trisulfide.
Detailed Description
The present invention will be described in further detail with reference to examples.
1. Experimental materials: 1. a compound: dimethyl trisulfide (liquid), available from Sigma, USA.
2. Biological material: except that the strain of the citrus green fungus (Penicillium digitatum) and the strain of the lychee downy mildew (Phytophthora litchii) are obtained by self-collection and separation, the other strains are all disclosed strains. The strain is preserved in the laboratory of the applicant and can be released externally.
2. The experimental operation process comprises the following steps:
1. after each plant pathogenic bacterial strain was cultured on PDA solid medium at 25℃for 5-7 days, a hole punch with a diameter of 6mm was used to punch the outer edge of the colony in order to keep the strain at the same viability.
2. 4 small culture dishes with the diameter of 6cm and the height of 1.5cm (the volume of about 35 mL) are placed in a large culture dish with the diameter of 15cm and the height of 3cm (the volume of about 500 mL), a tube cover of a 2mL centrifuge tube is placed in the center of the large culture dish, and a circular filter paper sheet with the diameter of 15mm multiplied by 15mm is placed in the centrifuge tube cover. After 4 small dishes were poured with 5mL of PDA medium, a 6mm diameter cake was inoculated in the center of the dish after solidification of the PDA medium, and then a corresponding volume of dimethyl trisulfide was added to the centrifuge tube cover in the center of the large dish, each concentration was added in an amount of 0. Mu.L/L (CK), 1. Mu.L/L (0.5. Mu.L/dish), 5. Mu.L/L (2.5. Mu.L/dish), 10. Mu.L/L (5. Mu.L/dish), 15. Mu.L/L (7.5. Mu.L/dish), 20. Mu.L/L (10. Mu.L/dish) and 40. Mu.L/L (20. Mu.L/dish), 3 replicates (i.e., 3 large dishes) were made for each concentration and a set of no dimethyl trisulfide was added as a control. Immediately after the addition of the dimethyl trisulfide, the mixture was sealed with a sealing film so as to prevent the dimethyl trisulfide from leaking.
3. After the treatment, the mycelia were observed and assayed for growth in an incubator at 25 ℃. When the control plate without drug was grown to the edge, colony diameters were measured by the crisscross method and inhibition rates were calculated. Inhibition (%) = (control hypha diameter-treated hypha diameter)/control hypha diameter×100 (see table 1). After the bacteriostasis rate is obtained, the DPS is used for calculating the virulence returnEquation(s) to (EC) 50 And EC (EC) 95 Values (see table 2).
TABLE 1 bacteriostasis of Dimethyltrisulfide at different concentrations against various pathogens%
Figure BDA0003727797280000041
Figure BDA0003727797280000051
Remarks: strains are derived from different hosts and have slightly different sensitivities to agents.
From the table, when 40 mu L/L dimethyl trisulfide is used, the antibacterial rate of fusarium oxysporum banana (Fusarium oxysporum f.sp.cube) is lower (38.39%), and the antibacterial rate of the fusarium oxysporum banana is more than 75% for other plant pathogenic bacteria; the antibacterial rate of the bacteria against a plurality of pathogenic bacteria reaches 100 percent; when 10 mu L/L dimethyl trisulfide is used, the antibacterial rate of the composition can reach 100% on rice blast fungus (Magnaporthe oryzae), corn rhizoctonia solani (Rhizoctonia solani), dragon fruit canker fungus (Neoscytalidium dimidiatum) and Botrytis cinerea (Botrytis cinerea); in particular, the bacteriostasis rate of 5 mu L/L dimethyl trisulfide to rhizoctonia solani (Rhizoctonia solani) and Botrytis cinerea (Botrytis cinerea) can reach 100%, and the bacteriostasis rate of 1 mu L/L dimethyl trisulfide to rhizoctonia solani (Rhizoctonia solani) can reach 89%.
TABLE 2 toxicity regression equation, EC, of dimethyl trisulfide against various plant pathogens 50 And EC (EC) 95 Value of
Figure BDA0003727797280000052
As can be seen from the calculation data in Table 2, the EC50 concentration of the dimethyl trisulfide for Rhizoctonia solani (Rhizoctonia solani) is 0.12 mu L.L -1 For Botrytis cinerea, the EC50 concentration is 0.85 mu L.L -1 。

Claims (5)

1. The application of dimethyl trisulfide in inhibiting plant pathogenic bacteria, such as Phytophthora litchi (Phytophthora litchi).
2. Use of dimethyl trisulfide according to claim 1, for inhibiting a phytopathogen, the host of which is litchi.
3. Use according to claim 1 for fumigation of phytopathogens with dimethyl trisulfide.
4. The use according to claim 3, wherein the concentration of dimethyl trisulfide used in the fumigation treatment is 0.1 μl/L to 40 μl/L.
5. The use according to claim 4, wherein the concentration of dimethyl trisulfide used in the fumigation treatment is 0.1-20. Mu.L/L.
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US2917429A (en) * 1956-08-17 1959-12-15 Collier Carbon & Chemical Co Method of destroying nematodes employing dimethyl polysulfides
CN106212507A (en) * 2016-08-31 2016-12-14 广西壮族自治区农业科学院植物保护研究所 NSC 97324 application in biocontrol of mango anthracnose

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