Disclosure of Invention
Aiming at the defects of the current black and odorous water body treatment technology, the invention provides the black and odorous water treatment system which is simple in structure, convenient to operate, high in treatment efficiency, simple in method and easy to operate.
The black and odorous water body treatment system is realized by the following modes:
a device for treating black and odorous water by using mixed culture denitrification coupled micro-nano aeration comprises a mixed culture denitrification unit and an aerobic micro-nano aeration unit, wherein the mixed culture denitrification unit and the aerobic micro-nano aeration unit are connected in series; wherein the polyculture denitrification unit enriches autotrophic denitrifying bacteria through hairbrush type fillers; the aerobic micro-nano aeration reactor divides the reaction zone into a clear liquid zone and a mixed liquid zone through a double-layer stainless steel mesh cylinder, and a sponge packing layer is arranged in a double-layer space of the double-layer stainless steel mesh cylinder; the gas-dissolved water is sucked into the micro-nano bubble generator through the filter in the clear liquid area, and the generated bubble liquid enters the bottom of the mixed liquid area; the middle of the mixed liquid area is provided with a brush, the middle shaft of the brush is connected with a stirring motor, and the brush is close to the inner stainless steel mesh cylinder for rotary scrubbing.
Furthermore, the polyculture denitrification reactor is enriched with autotrophic denitrifying bacteria through the brush filler of the anoxic tank, and the heterotrophic denitrifying bacteria are enriched in suspended sludge and overflow into the clear liquid zone of the aerobic micro-nano aeration reactor through the anoxic water outlet groove at the top.
Furthermore, the micro-nano bubble generator needs to suck gas-dissolving water from the clear liquid area and release micro-nano bubble liquid from the mixed liquid area.
Further, the outer clear liquid zone is divided into a clear liquid inlet zone and a clear liquid outlet zone by a clear liquid zone separation baffle, the effluent of the polyculture denitrification unit enters the clear liquid inlet zone, and the effluent of the micro-nano aeration aerobic reaction enters the clear liquid outlet zone; the water inlet pipe of the micro-nano bubble generator is provided with a tee joint which is respectively connected with the water inlet pipe, the water inlet clear liquid area filter and the water outlet clear liquid area filter, and dissolved air water is simultaneously sucked from different clear liquid areas.
Further, the base of the double-layer stainless steel net cylinder is fixed at the bottom of the reactor, the bottom of the sponge cylinder is fixed at the bottom of the reactor, the mesh size of the stainless steel net cylinder is 60-200 meshes, the thickness of the sponge layer filler is 10-40mm, and the density of the sponge is 18-25kg/m 3 And the average gap between the sponge layer and the stainless steel net cylinder is not more than 5 mm.
Furthermore, the micro-nano bubble water outlet pipe sequentially penetrates through the sponge cylinder packing layer and the inner stainless steel mesh cylinder from top to bottom to finally reach the bottom of the mixed liquid area, and the opening upwards releases bubble liquid.
Further, the brush hair of the brush that agitator motor connects is the heliciform and distributes, and the brush hair edge is close inlayer stainless steel mesh section of thick bamboo surface, can evenly clean a stainless steel mesh section of thick bamboo when the motor drives the brush and rotates.
Further, the polyculture denitrification unit comprises a reactor main body, an anoxic tank water outlet groove, an anoxic tank brush filler and an anoxic water outlet pipe; an anoxic tank hairbrush filler is arranged in the reactor main body, an anoxic tank water outlet groove is formed in the outer wall surface of the top of the reactor main body, and the anoxic tank water outlet groove is connected with the aerobic micro-nano aeration unit through an anoxic water outlet pipe; a zigzag cofferdam is arranged on one side of the anoxic pond water outlet groove, which is close to the reactor main body area; the brush filler adopts a three-dimensional elastic filler.
Further, the aerobic micro-nano aeration unit comprises an aerobic micro-nano aeration reactor, a rotatable brush, a stirring motor, a micro-nano bubble water outlet, a micro-nano dissolved air water inlet, a filter, a micro-nano bubble generator, an aerobic tank backflow outlet, an aerobic tank water outlet and a clear liquid zone separation baffle; a double-layer stainless steel mesh cylinder is arranged in the aerobic micro-nano aeration reactor and consists of an outer stainless steel mesh cylinder and an inner stainless steel mesh cylinder, and a sponge packing layer is filled between the outer stainless steel mesh cylinder and the inner stainless steel mesh cylinder; a clear liquid area separating baffle is arranged outside the double-layer stainless steel mesh cylinder to separate an outer clear liquid area into a water inlet clear liquid area and a water outlet clear liquid area; a rotatable brush is arranged at the center inside the double-layer stainless steel mesh cylinder and connected with a stirring motor; a filter is arranged in the effluent clear liquid area, the filter is connected with a micro-nano dissolved air water inlet of a micro-nano bubble generator through a pipeline, a micro-nano bubble water outlet of the micro-nano bubble generator is connected to the bottom position of the center of the inner part of the double-layer stainless steel mesh cylinder through a pipeline, and one end of the pipeline, which is positioned at the bottom position of the center of the inner part of the double-layer stainless steel mesh cylinder, is connected with a micro-nano bubble nozzle; the aerobic micro-nano aeration reactor is provided with an aerobic tank water outlet, and the aerobic tank water outlet is also connected with an aerobic tank reflux inlet of the polyculture denitrification unit.
Furthermore, an anoxic tank water inlet and an aerobic tank backflow inlet are formed in the bottom of the polyculture denitrification unit, and the anoxic tank water inlet is connected with the water storage tank through a pipeline; the return inlet of the aerobic tank is connected with the return outlet of the aerobic tank through a pipeline, and a water inlet pump is arranged on the pipeline between the water inlet of the anoxic tank and the water storage tank; a reflux pump is arranged on a pipeline between the aerobic tank reflux inlet and the aerobic tank reflux outlet.
In the invention, black odorous water enters from the bottom of the reactor through a water inlet pump, firstly, reflux liquid of a denitrification unit and a aerobic unit is mixed, and when COD (chemical oxygen demand) in the wastewater is sufficient, denitrifying bacteria are firstly used for heterotrophic denitrification, and the COD in the wastewater is rapidly consumed; then the waste water flows through the surface of the filler in the anoxic zone, and the residual nitrate nitrogen or nitrite nitrogen is subjected to autotrophic denitrification nitrogen removal under the action of sulfur autotrophic microorganisms on the filler. After passing through the denitrification unit, wastewater enters the aerobic nitrification unit through overflow, the aerobic unit performs high-efficiency aeration by adopting a micro-nano bubble technology, and the dissolved air water and the sludge contained in the mixed liquid have different concentrations, so that the aerobic unit is divided into a clear liquid area and a muddy water mixing area through a double-layer stainless steel mesh cylinder and a middle sponge packing layer, wherein the dissolved air water is sucked into the micro-nano bubble generator from the clear liquid area, and the bubble water is introduced into the muddy water mixing area from the micro-nano bubble generator. The middle of the muddy water mixing area is provided with a rotary brush, and the brush rotates to prevent the blockage caused by the adsorption of excessive sludge on the surface of the stainless steel mesh cylinder.
Compared with the prior art, the invention utilizes the denitrification unit which is formed by blending autotrophic denitrifying bacteria and heterotrophic denitrifying bacteria to couple the micro-nano aeration unit with the double-layer stainless steel mesh cylinder sponge middle layer structure. The invention can be used as an offshore technology for treating the black and odorous water body, the black and odorous water body is introduced into the system, and the mixed culture denitrification and the micro-nano aeration oxidation are carried out in the system, and compared with the independent autotrophic denitrification or heterotrophic denitrification, the mixed culture denitrification has better adaptability to the quality of the black and odorous water, and is particularly suitable for the denitrification of the black and odorous water body containing COD and sulfide; and the structure of the micro-nano aeration unit with the double-layer stainless steel mesh cylinder sponge middle layer structure can ensure that the bubble generator can continuously generate micro-nano bubbles under the condition of muddy water mixing, and can provide sufficient dissolved oxygen for the aerobic unit. Experiments prove that the system has obvious advantages compared with the traditional biological treatment process under the condition of controlling the same aeration quantity, and the black and odorous water treated and discharged by the system is still rich in micro-nano bubbles for a long time and can continuously and stably exist in the treated water body. In conclusion, the offshore treatment device can realize offshore treatment of the black and odorous water body, has an obvious water quality purification function, and has the advantages of small floor area, high pollutant removal efficiency, capability of providing persistent micro-nano bubbles under the condition of mixing muddy water and the like.
Detailed Description
To make the objects, technical solutions and advantages of the present invention more apparent, the embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The described embodiments are only some embodiments of the invention, not all embodiments.
Example 1
As shown in fig. 1-2, a mixed culture denitrification coupling micro-nano aeration black and odorous water treatment system is composed of a mixed culture denitrification anoxic unit and an aerobic micro-nano aeration unit which are connected in series; wherein the polyculture denitrification unit comprises an anoxic tank water outlet groove 1, an anoxic tank brush filler 2, an anoxic tank water inlet 3, an aerobic tank reflux inlet 4 and an anoxic water outlet pipe 5. One side of the anoxic pond water outlet groove, which is close to the reactor main body area, is provided with a zigzag cofferdam 6, and the brush packing is suitable for adopting a three-dimensional elastic packing. The black and odorous water to be treated is pumped into the anoxic tank 20 through an anoxic tank water inlet pump, the water flow flows upwards and sequentially flows through the mixed reaction zone and the hairbrush type carrier filler zone, enters the anoxic tank water outlet trough through the water outlet cofferdam after undergoing heterotrophic denitrification and autotrophic denitrification reactions, and finally enters the aerobic reactor through gravity flow. The aerobic reactor comprises a water inlet clear liquid area 9, a sponge packing layer 10, a rotatable brush 11, an outer stainless steel mesh cylinder 12, an inner stainless steel mesh cylinder 13, a micro-nano bubble nozzle 14, a water outlet clear liquid area 15, a stirring motor 23, a micro-nano bubble water outlet 17, a micro-nano dissolved air water inlet 18, a filter 16, a micro-nano bubble generator 19, an aerobic tank backflow outlet 21, an aerobic tank water outlet 22 and a clear liquid area separation baffle 24. Water flowing out of a water outlet pipe of the anoxic pond firstly enters a water inlet clear liquid area 9, then sequentially penetrates through an outer stainless steel mesh cylinder 12 and an inner stainless steel mesh cylinder 13 and a sponge filler middle layer 10 to enter an inner mixed liquid area, a rotatable brush 11 is arranged in the middle of the mixed liquid area, a motor 23 is connected with a center shaft of the brush, and the brush is close to the inner stainless steel mesh cylinder to rotate and scrub. In the rotating process of the brush, the sludge attached to the surface of the inner layer stainless steel mesh cylinder 13 is scoured off again, and the sludge is prevented from entering the sponge layer 10 through the inner layer stainless steel mesh cylinder 13 to cause the sludge loss of the mixed liquid reaction area. The micro-nano bubble liquid is released upwards through the micro-nano bubble liquid outlet 17 and the micro-nano bubble nozzle 14 to enter the mixed liquid reaction zone, and under the stirring of the rotatable brush 11, the wastewater in the mixed liquid zone and the sludge undergo aerobic degradation reaction. The dissolved gas water is sucked into the micro-nano bubble generator 19 through the filter 16 and the micro-nano dissolved gas water inlet 18 in the effluent clear liquid area 15. The water treated in the intermediate mixed liquid reaction zone flows through the inner stainless steel mesh cylinder 13, the sponge filler intermediate layer 10 and the outer stainless steel mesh cylinder 12 in sequence again and enters the effluent clear liquid zone 15, part of the wastewater enters the anoxic tank through the aerobic tank return pipe 21 and the anoxic tank return port 4 under the action of the return pump 8, and the rest wastewater is discharged through the aerobic tank water outlet 22.
The method for deeply treating black smelly water by adopting the device in the embodiment for preparing the black smelly water for laboratory simulation in the water storage tank 20 comprises the following steps:
and (3) turning on a water inlet pump 7 to enable black and odorous water to be treated to enter the polyculture denitrification anoxic tank, controlling the flow of inlet water to be 14.6mL/min, controlling the flow of reflux to be 29.2mL/min and controlling the reflux ratio to be 2:1, intermittently controlling the aeration time of the aerobic tank for the aerobic tank, adopting an aeration mode of 30min aeration and 30min interval, controlling the air inflow of conventional aeration and micro-nano aeration to be 100mL/min, supplying oxygen to the aerobic tank by using the conventional aeration at the initial operation stage, and replacing the operation with the micro-nano aeration mode after the operation is stable. The micro-nano bubble generator needs to suck gas dissolving water from a clear liquid area and release micro-nano bubble liquid from a mixed liquid area. The outer clear liquid zone of the aerobic micro-nano aeration unit is further divided into a clear liquid inlet zone and a clear liquid outlet zone by a baffle, the effluent of the polyculture denitrification unit enters the clear liquid inlet zone, and the effluent of the micro-nano aeration aerobic reaction enters the clear liquid outlet zone; the water inlet pipe of the micro-nano bubble generator is provided with a tee joint which is respectively connected with the water inlet pipe, the water inlet clear liquid area filter and the water outlet clear liquid area filter, and the dissolved air water is simultaneously sucked from different clear liquid areas.
The base of the double-layer stainless steel net cylinder is fixed at the bottom of the reactor, the bottom of the sponge cylinder is fixed at the bottom of the reactor, the mesh size of the stainless steel net cylinder is 120 meshes, the thickness of the sponge layer filler is 20mm, and the density of the sponge is 25kg/m 3 And the average gap between the sponge layer and the stainless steel net cylinder is 2 mm.
The micro-nano bubble water outlet pipe sequentially penetrates through the sponge cylinder packing layer and the inner stainless steel mesh cylinder from top to bottom to finally reach the bottom of the mixed liquid area, and the opening upwards releases bubble liquid.
The brush of vertical direction in the mixed liquid district be connected with the motor, the brush hair is the heliciform and distributes, the brush hair edge is close inlayer stainless steel net section of thick bamboo surface, can evenly clean a stainless steel net section of thick bamboo when the motor drives the brush and rotates.
Experimental example 1
The inoculation of the polyculture denitrification reactor adopts sulfur-oxidizing bacteria sludge and denitrification sludge, and the inoculation of the aerobic reactor adopts secondary sedimentation tank sludge of a sewage treatment plant. At the initial stage of the operation of the reactor, firstly, a mixed culture denitrification and conventional aerobic aeration combined mode is adopted for treatment, a continuous water inlet mode is adopted, after the water outlet operation of the reactor is stable, the aeration mode is changed into micro-nano bubble aeration at 34d, and then the operation is continued until the water outlet is stable. And in the operation stage, the air input of the conventional aeration and the air input of the micro-nano aeration are controlled to be consistent, the air input is 100mL/min, and an operation strategy of aerating for 30min at intervals of 30min is adopted.
COD removal
The COD removal rate of the black and odorous water body treated by the polyculture denitrification coupled micro-nano aeration is reduced to 29.3% in 0-10d at the initial stage of inoculation, and then the COD removal rate is gradually stabilized at 49.2 +/-6.8% in 11-33d (figure 3). After the second stage (34-52d) is changed to the micro-nano aeration mode, the COD level of the inlet water is maintained at 500-600mg/L, the COD concentration of the outlet water is reduced to 52.6mg/L, the COD removal rate of the total outlet water is increased from 56.8% to 90.8% at most, and the average COD removal rate is as high as 70.8%, which is obviously improved compared with the initial acclimation period. At 53-67d, in order to adopt the mixed culture denitrification coupling micro-nano aeration process to simulate and treat the general black and odorous water body, the water inlet load is correspondingly reduced to the level of 200 and 250mg/L, the COD removal rate is always maintained to be more than 85%, and the average value of the outlet water is 22.1mg/L, thereby reaching the COD standard of surface V-type water. The improvement of the COD removal rate promotes the aerobic degradation of COD in the wastewater due to the enhancement of oxygen supply, and generates more nitrite or nitrate due to the improvement of nitrification, thereby enhancing the process of removing COD by heterotrophic denitrification.
Ammonia nitrogen removal
In the initial stage of inoculation and the stable operation period of conventional aeration (0-33d), the removal rate of ammonia nitrogen is always kept in a lower range of 4.1-23.9% (figure 4). The reason is that DO under the conventional aeration is relatively lack, the nitrification or nitrosation reaction can not be ensured, after the micro-nano aeration is switched, the ammonia nitrogen concentration of the effluent water is gradually reduced in 34-45d and is reduced to 0.208mg/L from 43.27mg/L, the ammonia nitrogen removal rate is improved to be close to 99.6% from 20.2%, and in the subsequent 46-67d, the ammonia nitrogen removal rate is always in a higher level, wherein the average ammonia nitrogen removal rate is 93.3%, the average ammonia nitrogen of the effluent water is 1.54mg/L and is lower than the ammonia nitrogen standard of surface V-type water and the lowest ammonia nitrogen limit value of black and odorous water.
In the conventional aeration stage of 0-33d of mixed culture denitrification coupling, the removal rate of Total Nitrogen (TN) is always maintained at 9.8-24.7% (figure 5), the total nitrogen removal efficiency is low, the oxidation efficiency of ammonia nitrogen is low, and in the 0-33d, the removal rate of TN is almost consistent with the removal rate of ammonia nitrogen, which shows that in the conventional aeration stage, the oxidation of ammonia nitrogen is the main rate-limiting step. After the conventional aeration is replaced by the micro-nano aeration from 34d, the TN removal rate is obviously improved from 22.2 percent, and the highest removal rate is up to 79.0 percent. The micro-nano bubble aeration has more obvious ammonia nitrogen oxidation capacity and denitrification capacity compared with the conventional aeration.
In the conventional aeration operation stage, the DO value of the effluent is 0.51-1.82mg/L, and according to the classification standard of the urban black and odorous water body, the DO value of the effluent in the conventional aeration operation still reaches the classification standard of slight black and odorous water; after the micro-nano aeration is adopted, the DO of the effluent is in the range of 3.79-4.63mg/L, which is superior to the DO standard of the black and odorous water body. Meanwhile, due to the characteristic of stable and continuous existence of the micro-nano bubbles in water, the DO of the treated effluent is slowly attenuated, the DO can still keep 3-4mg/L after 72 hours of standing, the quality of the effluent no longer meets the standard of black and odorous water, and part of water quality indexes can reach the standard of urban surface V-type water.
The sulfide in the black and odorous water body treatment process is removed, the concentration of the sulfide in the water inlet of the reactor is 15mg/L level, the sulfide concentration of anoxic water outlet and aerobic water outlet is gradually reduced in the whole black and odorous water treatment stage, the anoxic unit plays a main role in removing the sulfide, the removal rate reaches 34.6% -55.1% (figure 6), and the removal rate of the anoxic unit on the sulfide accounts for 76.1% -100% of the total removal rate, which indicates that the sulfide is mainly removed in the anoxic unit through sulfur autotrophic bacteria oxidation. In the stage of 0-33d, the removal rate of the sulfide is increased from 11.74% to 73.5%, and after the micro-nano aeration is replaced, the removal rate of the sulfide is always kept above 95%. In the sulfur autotrophic denitrification reaction, the consumption of 2.51g S is required for denitrification removal of 1g N, namely, in the system, the heterotrophic denitrification reactor in the conventional aeration mode can remove 5.19-8.26mg/L of sulfide and 2.07-3.29mg/L of total nitrogen. And the polyculture denitrification reactor in the micro-nano aeration mode can remove 15mg/L of sulfide and 6mg/L of total nitrogen.
Experimental example 2
In the initial stage of the operation of the reactor, activated sludge in a secondary sedimentation tank of a sewage treatment plant is adopted as inoculated sludge in a conventional aerobic aeration unit, and after the operation of the effluent of the reactor is stable, the aeration mode is changed into micro-nano bubble aeration, and then the operation is continued until the effluent is stable.
Will be connected withAfter the seed sludge is inoculated in the aerobic unit, the MLSS value is obviously reduced. The sludge concentration after the initial inoculation is 3139.1mg/L (figure 7), and in the large bubble aeration period, less sludge enters the sponge layer, and the MLSS of the aerobic unit slowly drops to 2203.3mg/L within 30 d. The results show that there was little suspended sludge discharged from the reactor with the effluent, demonstrating that most of the sludge entered the sponge bed. After switching to MNBs aeration, MLSS dropped to 1204.7mg/L in 6 days and continued to drop to 38.2mg/L in 10 days. Finally, MLSS could not be detected in the aerobic mixed liquor zone within 50-68 d. Dissolved Oxygen (DO) was maintained at 0.4-0.7mg/L during the large bubble aeration period (FIG. 7). The results show that the effluent of the aerobic reactor is in an anoxic environment, and when the MNBs are switched for aeration, the DO of the effluent is rapidly increased from 0.4mg/L to 4.0 mg/L. Increase in DO with COD and NH 4 + The removal of-N promoted correspondingly (corresponding to experimental example 1). This result can be explained by the higher oxygen transfer efficiency of MNBs than with large bubble aeration.
The reduction of the MLSS value is caused by the generation mode of the gas-liquid mixed liquid of MNBs. A small amount of clarified liquid is required during the decompression release of dissolved gases (MNBs generation method), it is necessary to avoid the flow of Suspended Solids (SS) into the MNBs generator, and then the gas-liquid rich in MNBs is ejected through the outlet. The suspended floccules enter the sponge layer and are subsequently adsorbed onto the interstices of the sponge layer. In addition, MNBs enter the sponge space and interact with sludge microorganisms. Finally, as the MLSS value in the aerobic reactor is reduced, the flocculated sludge is gradually attached to the carriers. Although the MLSS value decreased, no microorganisms were lost from the reactor. The SS value of the effluent is below 30mg/L in the whole experimental process. This is therefore due to the effect of converting suspended sludge into biofilm sludge. In previous studies, it was also found that MNBs accelerate biofilm formation in biofilm reactors. In addition, the experiment also reveals that the MNBs can promote the conversion of suspended sludge into a biological membrane during aeration. Furthermore, as the MLSS value decreases, there is no adverse effect on the performance of the entire reactor. In conclusion, in the treatment of black and odorous water, MNBs promote the conversion of suspended sludge to a biological membrane and enhance the COD and NH 4 + The removal capacity of N and TN.
Experimental example 3
In the initial stage of the operation of the reactor, activated sludge is adopted as an inoculum in a conventional aerobic aeration unit, and after the operation of the effluent of the reactor is stable, the aeration mode is changed into micro-nano bubble aeration, and then the operation is continued until the effluent is stable. After the aeration mode is switched and the aeration is operated for a long time, the activated sludge microorganism populations in the anoxic unit and the aerobic unit are obviously influenced by MNBs, and the metabolic functions of sludge microorganisms are influenced accordingly.
During reactor operation, large bubble aerated and MNBs aerated sludge were collected from the anoxic unit for microbial community analysis on days 30 and 60, respectively, and represented by anoxic 1 and anoxic 2 samples, respectively. Sludge was collected from the aerobic unit on days 30 and 60, as indicated by large bubble sludge and MNBs sludge. On the portal level, the anoxic sludge in the large bubble aeration stage is dominated by proteobacteria, chlorobacteria, bacteroidetes and acidobacter (fig. 8 a). The anoxic sludge in the MNBs aeration stage mainly comprises proteobacteria, chlorella, bacteroidetes and epiphyte. The abundance of acidophyla in hypoxia 1 and 2 hypoxia 2 was 6.95% and 2.92%, respectively. Whereas in hypoxia 1 and 2, the abundance of the phylum Epsilonberaeota was 0.16% and 11.93%, respectively. At the genus level, anoxic sludge is dominated by thermomomonas (relative abundance of 8.06%), a genus of thermophilic bacteria found in waste activated sludge and denitrification reactors. During the aeration phase of MNBs, anoxic sludge is dominated by vibrio (11.45% relative abundance), a highly metabolically diverse genus of bacteria using various electron acceptors (e.g., oxygen, nitrate, and sulfur). The genus belongs to the phylum Episobacter, corresponding to an increase in the MNBs stage at the phylum level. In addition, the abundance of the well-known nitrite-oxidizing bacteria Nitrospira after aeration in an anoxic unit dropped from 1.42% to 0.1%. These results indicate the effect of MNBs aeration on anoxic sludge.
The effect of MNBs on the oxidation unit sludge microbial community is shown in FIG. 6. In the large bubble aeration phase, on the phylum level, large bubbles and MNBs sludge are dominated by proteobacteria, Chlorobacteria, Bacteroides and Acidobacterium. After aeration with MNBs, the abundance of acid bacteria in the aerobic sludge is increased from 9.00% to 16.4%. At the genus level (fig. 8b), the main bacteria of large vesicular sludge is brevundimonas (relative abundance of 8.80%), which is a common contaminant-degrading genus in sewage treatment plants. And in the aeration period of the MNBs, the dominant bacteria are respectively uncultured blastocyst family bacteria and uncultured SC-I-84 bacteria. By using a poly-organic as an electron donor, the blastocyst family was found to be responsible for nitrate reduction. The uncultured bacteria SC-I-84 were found in oligotrophic environments such as soil and sediments. These results indicate that MNBs significantly alter the microbial community of aerobic sludge.
Faperox and Bugbase based on 16SrRNA are used to predict the function of microorganisms in sludge. The functional microbial abundance of aerobic chemoheterotrophic, nitrification, nitrite respiration, aerobic nitrite oxidation, and aerobic ammonia oxidation of MNBs sludge was 44.2%, 80.5%, 103.2%, 82.8%, and 74.6% higher than that of macrovesicular sludge, respectively (fig. 9, panel a). The fermentation, sulfur compound respiration and sulfate respiration abundances of the large bubble sludge are respectively 184.2%, 79.6% and 72.0% higher than those of MNBs sludge. These results indicate that MNBs sludge has a high oxidizing capacity for organics, ammonia and nitrites. In addition, large bubble sludge has a high respiratory capacity for sulfate and organic matter fermentation, indicating that a relatively anaerobic environment exists in the sludge. Furthermore, MNBs do not differ significantly from the denitrification function of large bubble sludge (nitrate respiration, nitrogen reduction and nitrogen respiration). In addition, the results of Bugbase showed that there was a significant difference between the large bubble sludge and the MNBs sludge (panel b in fig. 9). MNBs sludge contains more gram-negative bacteria and less gram-positive bacteria. In addition, the biofilm formation potential in MNBs sludge was 9.37% higher than in large bubbles, indicating that MNBs promoted biofilm formation. This result is in agreement with the results of a previous study by Xiao et al on two different aerobic biofilm systems. At the same time, it was found that MNBs sludge also found a higher facultative anaerobic potential than large bubble sludge. This is because during the large bubble aeration phase, the sludge is in suspension and no significant biofilm formation occurs. In the aeration stage of MNBs, most sludge flocculating constituents are attached to the sponge layer and converted into biological membranes. Then, as the biofilm grows, aerobic bacteria and facultative anaerobic bacteria are distributed on the surface layer and the inner layer of the biofilm, respectively. The biofilm provides space for growth of facultative anaerobes and denitrifying bacteria. These results indicate that aeration of MNBs promotes biofilm formation, enhances oxidation of organics, ammonia nitrogen and nitrite, but has no negative impact on denitrification.
In the embodiment, the treatment effect of the black odorous water by adopting the polyculture denitrification coupling micro-nano aeration is obviously superior to that of the denitrification coupling conventional aeration, wherein COD and ammonia nitrogen of the effluent reach the standard of surface V-type water, DO of the effluent reaches 4.63mg/L, ORP reaches 280-340 mv, and S reaches 280-340 mv 2- The removal rate of the sludge reaches 100%, the micro-nano aeration can promote the conversion of suspended sludge to biofilm sludge, synchronously strengthen the removal of COD, ammonia nitrogen and TN, and enhance the oxidation of organic matters, ammonia nitrogen and nitrite, but has no negative influence on denitrification. The quality of the treated effluent is superior to the standard of black and odorous water, black and odorous water can be eliminated, and COD and ammonia nitrogen indexes can completely reach the standard of urban surface V-type water. In conclusion, the system can be suitable for offshore treatment of black smelly water, has a remarkable water quality purification function, and is high in system operation stability and treatment efficiency.