CN115024851A - Automatic change spinal cord injury animal model preparation facilities - Google Patents
Automatic change spinal cord injury animal model preparation facilities Download PDFInfo
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- CN115024851A CN115024851A CN202210914148.0A CN202210914148A CN115024851A CN 115024851 A CN115024851 A CN 115024851A CN 202210914148 A CN202210914148 A CN 202210914148A CN 115024851 A CN115024851 A CN 115024851A
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- A61D1/00—Surgical instruments for veterinary use
Abstract
The invention discloses an automatic preparation device of a spinal cord injury animal model, which comprises an operation table, wherein the operation table comprises a base, a horizontal moving device and a vertical moving device, wherein the horizontal moving device and the vertical moving device are arranged on the upper surface of the base; the spinal cord impactor is arranged on the vertical moving device and is used for impacting the spinal cord to form injury; the distance measuring positioner is arranged on the vertical moving device and is used for positioning the impact part and automatically measuring the impact depth; a spinal fixator mounted on the horizontal movement device for fixing the spinal column of the animal so as to expose and position the spinal cord; and the control device is provided with a control panel and is used for setting and driving relevant working parameters of all the instruments and controlling the instruments to work. Therefore, the automatic impact preparation of the spinal cord injury animal model can be realized, and the accuracy and the objectivity are achieved. The device has simple structure, simple and convenient operation, light whole weight and convenient carrying.
Description
Technical Field
The invention relates to the technical field of experimental research in the field of neurobiology, in particular to an automatic preparation device for a spinal cord injury animal model.
Background
Spinal cord injury often results in permanent functional deficits below the injured segment of the patient, severely impacting the quality of life of the patient. How to repair the spinal cord injury is a worldwide problem for a long time, and at present, the treatment of the spinal cord injury mainly comprises early surgical decompression, drug treatment, stem cell treatment and the like, and no definite treatment method capable of improving clinical symptoms exists. Therefore, the research on the spinal cord injury through an ideal animal model has great social significance. The most common causes of spinal cord injury are car accidents, high-altitude falling and other injuries, dislocation after spine fracture or broken bone entering vertebral canal, and spinal cord or spinal nerve injury. The spinal cord injury animal models commonly used in the related researches at present generally include a spinal cord impact injury model, a spinal cord cutting injury model, a spinal cord forceps and clamping compression model, a spinal cord traction injury model and the like. The study of spinal cord injury through animal models requires certain clinical similarity, and the spinal cord impact injury model damages the spinal cord through impact, which is similar to most of the clinical traumatic spinal cord injuries. However, preparing a spinal cord injury model by impingement also requires the following features: (1) repeatability: animal models with consistent damage degrees are stably prepared, and the reliability is high. (2) Controllability: and adjusting the damage degree according to the experimental requirement. (3) Operability: the operation steps are convenient and simple, and the operation process is objective. At present, various spinal cord injury percussion devices have certain limitations and cannot have the three characteristics. Therefore, the automatic preparation device for the spinal cord injury animal model, which has high accuracy, strong repeatability and convenient operation, is established, and is beneficial to solving the related problems in the research of the spinal cord injury animal.
Disclosure of Invention
The invention aims to solve the problems and the defects, and provides an automatic preparation device for a spinal cord injury animal model, so as to solve the problems of insufficient accuracy, poor repeatability, complex operation and the like in the preparation process of the spinal cord injury animal model.
The technical scheme of the invention is realized as follows:
the invention discloses an automatic preparation device of a spinal cord injury animal model, which is characterized by comprising the following components: the operation table comprises a base, a horizontal moving device and a vertical moving device, wherein the horizontal moving device is arranged on the upper surface of the base, the vertical moving device is vertical to the horizontal moving device, and driving motors are arranged on the horizontal moving device and the vertical moving device; the spinal cord impactor is arranged on the vertical moving device and is used for impacting the spinal cord to form injury; the distance measuring positioner is arranged on the vertical moving device and is used for positioning an impact part and automatically measuring the impact depth; a spinal fixator mounted on the horizontal movement device for fixing the spine of the animal so as to expose and position the spinal cord; and the control device is provided with a control panel and is used for setting relevant working parameters of the driving motor, the spinal cord impactor and the distance measuring positioner and controlling the driving motor, the spinal cord impactor and the distance measuring positioner to work.
In some embodiments, the present invention further comprises a stage mounted on the horizontal movement device and horizontally movable in a direction perpendicular to the movement direction of the horizontal movement device, the spine fixator being mounted on the stage.
Furthermore, a manual control adjuster for manually adjusting and controlling the object stage to horizontally move relative to the horizontal moving device is arranged on the object stage.
In some embodiments, the vertical moving device comprises a vertical column, a vertical screw and a cantilever, wherein the vertical column is fixedly connected to the operating platform; the vertical screw is arranged on the side surface of the vertical upright column facing the direction of the horizontal moving device, one end part of the vertical screw is connected with the driving motor, and the driving motor drives the vertical screw to rotate; the first end of cantilever is equipped with the screw thread through-hole, the screw thread through-hole with perpendicular screw rod connects soon, just the terminal surface of first end with the side of perpendicular stand is connected with sliding from top to bottom, the second end orientation of cantilever is on a parallel with horizontal migration device moving direction's direction level extends, the spinal cord impinger with the range finding locator is installed second end lower extreme.
In some embodiments, the spinal cord impactor comprises an impacting head, a sliding seat and an impacting driving device, wherein the impacting head is detachably fixed on the lower surface of the sliding seat, the sliding seat is vertically and slidably arranged on a cantilever of the vertical moving device, the impacting driving device is fixed on the cantilever, and the impacting driving device drives the sliding seat to vertically slide.
Further, the impact driving device comprises a push rod, a contraction spring and an electromagnetic driving piece, wherein the electromagnetic driving piece is fixed on the cantilever, and the push rod is connected with the electromagnetic driving piece and drives the push rod to vertically move downwards through the electromagnetic driving piece; the lower end of the push rod is connected with the sliding seat; the contraction spring is arranged at the upper end of the push rod, the contraction spring is extruded to store energy when the push rod vertically moves downwards, after the electromagnetic driving piece stops driving, the contraction spring releases energy to drive the push rod to vertically move upwards, and the sliding seat and the impact head synchronously reset upwards.
In some embodiments, the horizontal moving device includes a horizontal base, a horizontal screw, and a sliding base, wherein the horizontal base is fixedly connected to an upper end surface of the operating platform; the horizontal screw is arranged on the horizontal base body, one end part of the horizontal screw is connected with the driving motor, and the driving motor drives the horizontal screw to rotate; the sliding seat is provided with a thread through hole, the thread through hole is connected with the horizontal screw rod in a screwing mode, the bottom surface of the sliding seat is connected with the bottom surface of the horizontal seat body in a horizontally sliding mode, and the spine fixator is installed on the top surface of the sliding seat.
In some embodiments, the distance measuring locator is a laser displacement sensor, the laser displacement sensor emits laser to irradiate the spinal fixator, the position irradiated by the laser is the position where the spinal impactor impacts the spinal cord, and the spinal fixator is moved by operating the horizontal moving device, so as to accurately position the impacted position of the spinal cord.
In some embodiments, relevant operating parameters of the spinal cord impactor include impact velocity, impact depth, and impact dwell time.
Furthermore, the control panel is a touch panel, after the relevant working parameters of the spinal cord impactor are set through the touch panel and started, the distance measuring positioner automatically measures the impact depth and then automatically adjusts the height of the spinal cord impactor, and the spinal cord is automatically impacted after the adjustment is completed.
The invention has the beneficial effects that:
1. the invention relates to an automatic preparation device of a spinal cord injury animal model, which utilizes an operation device to control an operation table, a spinal cord impactor and a distance measuring positioner, realizes automatic operation, can accurately position an impact part, effectively reduces manual operation errors, stably prepares the animal model with consistent injury degree and has high reliability and repeatability;
2. the control device digitally controls each relevant working parameter, so that the controllability is high, the accuracy and the objectivity are realized, the spinal cord injury degree can be adjusted according to the experimental requirements by changing the working parameters, and models with different injury degrees can be simulated;
3. the spine of the animal is fixed manually through the spine fixer, and after the impacted part of the spinal cord is positioned through the ranging positioner, the automatic impact can be carried out through the control device, so the operation steps are simple and convenient, and the operation process is objective;
4. the whole structure is arranged on the operation table, and the device has small volume, light weight and convenient carrying.
The invention will be further described with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic perspective view of an apparatus for preparing an automated spinal cord injury animal model according to the present invention;
FIG. 2 is a schematic exploded view of an apparatus for preparing an automated spinal cord injury animal model according to the present invention;
fig. 3 is a schematic perspective view of a vertical movement device according to an embodiment of the present invention;
FIG. 4 is a schematic perspective view of the cantilever arm connected to the spinal cord impactor and the distance measuring locator according to the present invention;
fig. 5 is a schematic perspective view of a horizontal movement apparatus according to an embodiment of the present invention;
FIG. 6 is a schematic perspective view of an object stage according to an embodiment of the present invention;
FIG. 7 is a schematic exploded view of a spinal cord impactor in accordance with an embodiment of the invention;
fig. 8 is a schematic perspective view of a distance measuring locator according to an embodiment of the invention.
Reference numerals:
a base 1;
a vertical movement device 2;
the vertical column 21, the vertical screw 22, the cantilever 23, the connecting plate 24, the vertical guide groove 25, the vertical guide rail 26 and the square hole 27; a T-shaped plate 28;
a horizontal movement device 3;
the horizontal base 31, the end plate 311, the bottom plate 312, the horizontal screw 32, the slide seat 33, the horizontal guide rail 34 and the horizontal guide groove 35;
a spinal cord impactor 4;
the impacting head 41, the bump 411, the impacting tip 412, the sliding seat 42, the impacting driving device 43, the push rod 431, the electromagnetic driving part 432, the convex cap 433, the nut 434, the sliding rail 44 and the sliding groove 45;
a distance measuring locator 5;
an object stage 6;
a connecting seat 61, a mobile station 62 and a manual control regulator 63;
a spinal fixation device 7;
a fixator base 71, spinal clamping jaws 72, fixator screw holes 73;
a manipulation device 8;
a touch panel 81;
the motor 9 is driven.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. In addition, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature for distinguishing between descriptive features, non-sequential, and non-trivial.
In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "fit," "connect," and "attach" are to be construed broadly and may include, for example, a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following describes an automatic spinal cord injury animal model preparation device of the present invention with reference to the accompanying drawings.
As shown in fig. 1 and 2, the invention relates to an automatic preparation device for a spinal cord injury animal model, which comprises an operation table, a spinal cord impactor 4, a distance measuring locator 5, a spinal column fixer 7 and an operation control device 8, wherein the operation table comprises a base 1, a horizontal moving device 3 arranged on the upper surface of the base 1 and a vertical moving device 2 perpendicular to the horizontal moving device 3, the spinal cord impactor 4 and the distance measuring locator 5 are connected to the vertical moving device 2, the spinal column fixer 7 is connected to the horizontal moving device 3, and the operation control device 8 is connected to the base 1.
The horizontal moving device 3 and the vertical moving device 2 are both provided with a driving motor 9, and the control device 8 simultaneously controls the driving motor 9, the spinal cord impactor 4 and the distance measuring positioner 5 to work, so that automatic work can be realized; the distance measuring and positioning device 5 can be used for positioning the part to be impacted by the spinal cord impactor 4 and automatically measuring the impact depth, so that the operation device 8 is used for controlling all relevant working parameters, the animal models with consistent injury degrees can be stably prepared, the reliability and the repeatability are high, the spinal cord injury degrees can be adjusted according to experimental requirements by changing the working parameters while the accuracy and the objectivity are realized, and models with different injury degrees are simulated.
As shown in fig. 2, the base 1 of the console of the present invention is designed as a rectangular base 1, the periphery of the rectangular base 1 is enclosed by a section bar 12, the top surface is a mounting panel 11, the horizontal moving device 3 and the vertical moving device 2 are both disposed on the upper surface of the mounting panel 11 of the base 1, and related electronic components are disposed under the mounting panel 11. The horizontal moving device 3 is horizontally arranged in the middle of the installation panel 11 along the Z-axis direction, the vertical moving device 2 is vertically arranged at one end part of the horizontal moving device 3 and is also positioned at the middle position of the installation panel 11 in the X-axis direction, and the horizontal moving device and the vertical moving device are both fixed on the installation panel 11 through bolts, so the center of gravity of the whole operation table is concentrated at the middle position after installation, and the phenomenon that the center of gravity is not centered in the use process and is easy to deviate can be avoided. The driving motor 9 of the horizontal movement device 3 is connected to the end position of the horizontal movement device 3 close to the vertical movement device 2, and the driving motor 9 of the vertical movement device 2 is arranged above the driving motor 9 of the horizontal movement device 3, so that the gravity of the two driving motors 9 is more concentrated on the middle position of the base 1, and the two driving motors 9 are closer to each other, thereby facilitating the circuit wiring and the electric connection with the control device 8.
As shown in fig. 3, the vertical moving device 2 of the present invention comprises a vertical column 21, a vertical screw 22 and a cantilever 23, wherein the bottom of the vertical column 21 is fixedly connected to the operation table by bolts; on the side of the vertical column 21 facing the direction of the horizontal moving device 3, a connecting plate 24 extends from each of the upper and lower ends, the upper end of the vertical screw 22 is rotatably connected to the upper end connecting plate 24, in some embodiments, a circular hole may be formed in the connecting plate 24, a bearing is installed in the circular hole, the upper end of the vertical screw 22 is sleeved in the bearing hole, so that the vertical screw 22 can rotate relative to the connecting plate 24, the lower end of the vertical screw 22 passes through the lower end connecting plate 24 and then is connected to the driving motor 9, and the driving motor 9 is a stepping motor and can drive the vertical screw 22 to rotate. In some embodiments, the cantilever 23 is a substantially rectangular bar-shaped arm, the first end of the cantilever 23 is provided with a threaded through hole penetrating along the Y-axis direction, the threaded through hole is screwed with the vertical screw 22, and the end surface of the first end along the Z-axis direction contacts with the side surface of the vertical column 21 facing the horizontal moving device 3 direction, so that the rotation of the cantilever 23 along with the vertical screw 22 is limited, so that the cantilever 23 can slide up and down after the vertical screw 22 rotates, further, the end surface of the first end along the Z-axis direction is provided with a vertical guide slot 25, and the side surface of the vertical column 21 facing the horizontal moving device 3 direction is provided with a vertical guide rail 26, and the vertical guide slot 25 and the vertical guide rail 26 are matched to realize the up and down sliding of the cantilever 23 under the rotation of the vertical screw 22. The second end of the cantilever 23 extends horizontally toward a direction parallel to the moving direction of the horizontal moving device 3 (i.e., the Z-axis direction in the drawing).
As shown in fig. 4, the spinal cord impactor 4 and the distance measuring locator 5 of the present invention are mounted at the second end of the cantilever 23. Specifically, the second end of the cantilever 23 is provided with a square hole 27 penetrating along the Y-axis direction, the upper part of the spinal cord impactor 4 passes through the square hole 27, and is screwed with a bolt through the side wall of the square hole 27 for fixing; the distance measuring locator 5 is connected to the bottom surface of the second end of the cantilever 23 and is located at the rear end of the spinal cord impactor 4, and two sides of the top of the distance measuring locator 5 are fixedly connected with the bottom surface of the second end of the cantilever 23 through bolts.
In some embodiments, the vertical moving device 2 can also realize vertical displacement by means of gear and rack transmission, and the vertical moving device 2 in this embodiment includes a vertical column 21, a cantilever 23, a rack, a gear and a driving motor 9, wherein the vertical column 21 is provided with a sliding slot, the rack is connected in the sliding slot in a way of moving up and down, and the cantilever 23 is fixedly connected to the back of the rack and extends out of the sliding slot; the gear is connected to the vertical column 21 in a rotatable manner, and the gear is engaged with the teeth of the rack, and the driving motor 9 is engaged with the gear through the transmission gear to realize power transmission. In other embodiments, pulleys may also be used for power transmission.
As shown in fig. 5, the horizontal moving device 3 includes a horizontal base 31, a horizontal screw 32 and a sliding base 33, wherein the horizontal base 31 is composed of two end plates 311 in the Z-axis direction and a bottom plate 312 located between the two end plates 311 and attached to the mounting panel 11 of the base 1, and the bottom plate 312 is fixedly connected to the mounting panel 11 through bolts; the horizontal screw 32 is arranged between the two end plates 311 of the horizontal seat body 31, the horizontal screw 32 is parallel to the bottom plate 312 and has a certain distance from the bottom plate 312, the horizontal screw 32 can rotate, one end part of the horizontal screw 32 is connected with the driving motor 9, the driving motor 9 is also a stepping motor and can drive the horizontal screw 32 to rotate; the sliding base 33 is provided with a threaded through hole, the threaded through hole is screwed with the horizontal screw 32, and the bottom surface of the sliding base 33 is horizontally slidably connected with the bottom surface of the horizontal seat body 31, in some embodiments, two horizontal guide rails 34 are provided on the bottom plate 312, two horizontal guide grooves 35 are provided on the bottom surface of the sliding base 33, and the horizontal guide rails 34 and the horizontal guide grooves 35 are matched with each other, so that the seat is driven to move along the Z-axis direction when the horizontal screw 32 rotates. The spine fixator 7 of the invention is arranged on the top surface of the sliding seat 33, so that the movement of the sliding seat 33 can drive the spine fixator 7 to move, and the part of the animal spine to be impacted fixed on the spine fixator 7 can be accurately moved to the position right below the spinal cord impactor 4 through the positioning of the distance measuring positioner 5.
As shown in fig. 6, in order to facilitate the installation and fixation of the spine fixator 7, the present invention further includes an object stage 6, the sliding base 33 is a rectangular block, the object stage 6 is installed on the sliding base 33 of the horizontal moving device 3 and is fixedly connected with the sliding base 33 through a bolt, and the spine fixator 7 is detachably installed on the object stage 6. Further, in order to achieve more accurate positioning of the spinal cord impact position, the object stage 6 includes a connecting seat 61 and a moving table 62, the connecting seat 61 is fixedly connected with the sliding seat 33, the moving table 62 is mounted on the connecting seat 61 in a manner of being horizontally movable along a direction perpendicular to a moving direction of the horizontal moving device 3 (i.e. an X-axis direction in the drawing), and the spinal column fixer 7 is mounted on the moving table 62, so that the spinal column fixer 7 can be horizontally moved along a Z-axis through the horizontal screw rod 32, and the spinal column fixer 7 can be horizontally moved along an X-axis through the moving table 62, so that spinal cord impact can be performed on any surface position of the spinal column of the animal clamped on the spinal column fixer 7.
Furthermore, in order to facilitate adjustment of the movable stage 62, the present invention provides a manual control actuator 63 for manually adjusting and controlling the horizontal movement of the stage 6 with respect to the horizontal movement device 3, wherein the manual control actuator 63 is a screw type actuator, and the precise displacement operation in the X-axis direction of the movable stage 62 can be realized. The manual control actuator 63 is integrally formed with the movable stage 62, preferably a precision manual displacement platform known as LX 80-R.
As shown in fig. 7, the spinal cord impactor 4 of the present invention comprises an impacting head 41, a sliding seat 42, and an impacting driving device 43, wherein the impacting head 41 is detachably fixed on the lower surface of the sliding seat 42, specifically, in some embodiments, the bottom of the sliding seat 42 is provided with an upwardly concave recess, and the impacting head 41 is composed of an upper protrusion 411 and a lower impacting tip 412, wherein the protrusion 411 is embedded in the recess of the sliding seat 42 and is locked and fixed by a screw. In other embodiments, the sliding seat 42 may be integrally formed with the striking head 41; or the bottom of the sliding seat 42 extends downwards to form a cylinder, a radial cylinder hole is formed in the cylinder, then the impacting head 41 is designed to be needle-shaped, the upper part of the impacting head is directly inserted into the cylinder hole, and the impacting head 41 is pulled down when the impacting head is replaced.
The sliding seat 42 of the present invention is vertically slidably provided on the arm 23 of the vertical movement device 2. Specifically, a T-shaped plate 28 extending downward along the Y axis is arranged on the bottom surface of the cantilever 23 between the spinal cord impactor 4 and the distance measuring locator 5, a slide rail 44 is connected to the front end of the T-shaped plate 28, a slide groove 45 is arranged on the end surface of the corresponding slide seat 42 facing the direction of the T-shaped plate 28, and the slide rail 44 is matched with the slide groove 45, so that the consistency of each vertical slide of the slide seat 42 can be ensured, no deviation occurs, and the repeatability and consistency of the preparation can be further improved.
As shown in fig. 4 or fig. 7, the impact driving device 43 of the present invention is fixed on the cantilever 23, specifically, the impact driving device 43 passes through the square hole 27 at the second end of the cantilever 23, and then is fixed by screwing a bolt through the side wall of the square hole 27, and the impact driving device 43 is used for driving the sliding seat 42 to slide vertically. Further, in some embodiments, the impact driving device 43 is a push-pull electromagnetic push rod 431 device, which includes a push rod 431, a retraction spring (not shown in the figure), and an electromagnetic driving member 432, wherein the electromagnetic driving member 432 is fixed on the cantilever 23, the push rod 431 is connected to the electromagnetic driving member 432, and the push rod 431 is driven by the electromagnetic driving member 432 to move vertically downward; the upper end of the push rod 431 is located above the electromagnetic driving element 432, the lower end of the push rod 431 passes through the electromagnetic driving element 432 and then is connected with the sliding seat 42, in some embodiments, a nut 434 is fixedly or rotatably connected to the top of the sliding seat 42, and the lower part of the push rod 431 is provided with external threads, by rotating the push rod 431 or the nut 434, the lower part of the push rod 431 can be screwed to the sliding seat 42, and the sliding seat 42 can synchronously move up and down along with the push rod 431; in other embodiments, the fixing can be performed by welding or side pin plugging. The retraction spring is disposed at the upper end of the push rod 431 between a convex cap 433 at the top end of the push rod 431 and the upper surface of the housing of the electromagnetic driver 432. According to the following operation principle, when the electromagnetic driving part 432 works, the push rod 431 is driven to vertically move downwards, the contraction spring is extruded to store energy at the moment, and the push rod 431 drives the sliding seat 42 to move downwards so as to drive the impact head 41 to impact downwards; when the electromagnetic driving element 432 stops working or works reversely, the contracting spring releases energy to drive the push rod 431 to move vertically upwards, or the reverse magnetic force is combined with the contracting spring release energy to simultaneously drive the push rod 431 to move vertically upwards, so that the sliding seat 42 and the impact head 41 are synchronously reset upwards.
In some embodiments, the range finder 5 is a laser displacement sensor, which is a sensor using laser technology for measurement, consisting of a laser, a laser detector and a measuring circuit. The laser displacement sensor has the functions of laser positioning and distance measurement. The laser emitted by the laser displacement sensor irradiates on the spine fixer 7, and the position irradiated by the laser is the position where the spinal cord impactor 4 impacts the spinal cord, so that the spine fixer 7 can be moved by operating the displacement of the horizontal moving device 3 and the moving platform 62, and the position where the spine is impacted can be accurately positioned. In addition, the distance measuring locator 5 can also be formed by combining an infrared locator and a distance measuring instrument; or other devices that may perform both location and ranging functions.
In some embodiments, as shown in fig. 8, the spine fixation device 7 may be a commercially available product, and the spine fixation device 7 includes a fixation device base 71 and a spine clamping jaw 72 disposed on the fixation device base, and a fixation device screw hole 73 is disposed on the bottom of the fixation device base 71, and the fixation device screw hole 73 matches with the screw hole on the movable table 62 and is screwed and fixed by a bolt. The spine fixator 7 can be divided into a rat spine fixator and a mouse spine fixator.
In some embodiments, the control panel of the control device 8 is a touch panel 81, and the control panel 81 is used to set parameters related to instruments such as the driving motor 9, the spinal cord impactor 4, the distance measuring locator 5, and the like, and control these instruments, which specifically include: impact speed, impact depth, impact dwell time, precise control of the stage 6, moving the spinal cord to the impact head 41 in preparation for impact and initiating impact, etc.
In application, the skin and soft tissue of animal are cut to expose spine; fixing the animal with the spinal fixator 7; opening the corresponding vertebral plate with an animal surgical instrument; fixing the spinal fixator 7 on the object stage 6; accurately positioning the impact part by using the touch control panel and the fine manual control adjuster 63 and referring to the distance measuring positioner 5; setting impact parameters by using a touch control panel; clicking a preparation button on the touch panel 81, adjusting the height of the impact head 41 after the distance measuring locator 5 automatically measures the impact depth, and moving the impact part to the impact head 41; clicking the start button on the touch panel 81 automatically strikes the spinal cord.
The following is a detailed description of specific embodiments.
As shown in fig. 1 to 8, the automatic preparation device for a spinal cord injury animal model of this embodiment includes an operation table, a spinal cord impactor 4, a distance measuring locator 5, a stage 6, a spinal fixation device 7 and a manipulation device 8, the operation table includes a base 1, a horizontal movement device 3 and a vertical movement device 2 which are arranged on the upper surface of the base 1, the spinal cord impactor 4 and the distance measuring locator 5 are arranged on the vertical movement device 2, the stage 6 is arranged on the horizontal movement device 3, the spinal fixation device 7 is connected to the stage 6, and the manipulation device 8 is arranged on the upper surface of the base 1.
The vertical moving device 2 comprises a vertical upright post 21, a vertical screw 22 and a cantilever 23, the bottom of the vertical upright post 21 is fixedly connected on the operation table through bolts, a connecting plate 24 respectively extends from the upper end and the lower end of the vertical upright post 21 towards the direction of the horizontal moving device 3, a round hole is arranged on the upper end connecting plate 24, a bearing is arranged in the round hole, the upper end of the vertical screw 22 is sleeved in the bearing hole, so that the vertical screw 22 can rotate relative to the connecting plate 24, the lower end of the vertical screw 22 passes through the lower end connecting plate 24 and then is connected with a driving motor 9, the driving motor 9 is a stepping motor and can drive the vertical screw 22 to rotate, the cantilever 23 is a rectangular strip-shaped arm, a threaded through hole penetrating along the Y-axis direction is arranged at the first end part of the cantilever 23, the threaded through hole is screwed with the vertical screw 22, and a vertical guide groove 25 is arranged at the end surface of the first end part along the Z-axis direction, a vertical guide rail 26 is arranged on the side surface of the vertical upright 21 facing the direction of the horizontal moving device 3, and the cantilever 23 slides up and down under the rotation of the vertical screw 22 through the cooperation of the vertical guide groove 25 and the vertical guide rail 26.
The spinal cord impactor 4 comprises an impacting head 41, a sliding seat 42 and an impacting driving device 43, wherein the bottom of the sliding seat 42 is provided with an upward concave position, the impacting head 41 consists of an upper convex block 411 and a lower impacting tip 412, and the convex block 411 is embedded in the concave position of the sliding seat 42 and is locked and fixed through screws; the bottom surface of the cantilever 23 is provided with a T-shaped plate 28 extending downwards along the Y axis between the spinal cord impactor 4 and the distance measuring locator 5, the front end of the T-shaped plate 28 is connected with a slide rail 44, the corresponding end surface of the sliding seat 42 facing the direction of the T-shaped plate 28 is provided with a sliding groove 45, the sliding seat 42 vertically slides through the matching of a sliding rail 44 and the sliding groove 45, the impact driving device 43 is fixed on the cantilever 23, specifically, the impact driving device 43 passes through the square hole 27 at the second end part of the cantilever 23, and then is screwed with a bolt through the side wall of the square hole 27 for fixing, the impact driving device 43 is used for driving the sliding seat 42 to slide vertically, the impact driving device 43 is a push-pull type electromagnetic push rod 431 device, the device comprises a push rod 431, a retraction spring (not shown in the figure) and an electromagnetic driving part 432, wherein the electromagnetic driving part 432 is fixed on the cantilever 23, the push rod 431 is connected with the electromagnetic driving part 432, and the push rod 431 is driven by the electromagnetic driving part 432 to vertically move downwards; the upper end of the push rod 431 is located above the electromagnetic driving part 432, the lower end of the push rod 431 is provided with an external thread (not shown in the figure), the top of the sliding seat 42 is fixedly connected with a nut 434, and the lower part of the push rod 431 is screwed on the sliding seat 42 by rotating the push rod 431; the retraction spring is disposed at the upper end of the push rod 431 between a convex cap 433 at the top end of the push rod 431 and the upper surface of the housing of the electromagnetic drive 432.
The distance measuring and positioning device 5 is a laser displacement sensor, is connected to the bottom surface of the second end part of the cantilever 23 and is positioned at the rear end of the spinal cord impactor 4, the two sides of the top of the distance measuring and positioning device 5 are fixedly connected with the bottom surface of the second end part of the cantilever 23 through bolts, laser emitted by the laser displacement sensor irradiates the spinal cord fixator 7, and the position irradiated by the laser is the position where the spinal cord impactor 4 impacts the spinal cord, so that the spinal cord fixator 7 can be moved through the displacement of the operation horizontal moving device 3 and the moving platform 62, and the accurate positioning of the position where the spinal cord is impacted is realized.
The horizontal moving device 3 comprises a horizontal seat body 31, a horizontal screw 32 and a sliding seat 33, wherein the horizontal seat body 31 consists of two end plates 311 in the Z-axis direction and a bottom plate 312 positioned between the two end plates 311 and attached to the installation panel 11 of the base 1, and the bottom plate 312 is fixedly connected with the installation panel 11 through bolts; the horizontal screw 32 is arranged between the two end plates 311 of the horizontal seat body 31, the horizontal screw 32 is parallel to the bottom plate 312 and has a certain distance from the bottom plate 312, the horizontal screw 32 can rotate, one end part of the horizontal screw 32 is connected with the driving motor 9, the driving motor 9 is also a stepping motor and can drive the horizontal screw 32 to rotate; the sliding seat 33 is provided with a threaded through hole, the threaded through hole is connected with the horizontal screw 32 in a screwing mode, the bottom plate 312 is provided with two sliding rails 44, the bottom surface of the sliding seat 33 is provided with two sliding grooves 45, and the sliding rails 44 are matched with the sliding grooves 45, so that the sliding seat is driven to move along the Z-axis direction when the horizontal screw 32 rotates.
The object table 6 is mounted on the slide 33 of the horizontal moving means 3 and fixedly connected to the slide 33 by means of bolts, and the spinal fixator 7 is removably mounted on the object table 6, the stage 6 includes a connecting base 61, a moving table 62 and a manual control adjuster 63, the connecting base 61 is fixedly connected with the slide base 33, the moving table 62 is horizontally movably mounted on the connecting base 61 in a direction perpendicular to a moving direction of the horizontal moving device 3 (i.e., an X-axis direction in the drawing), the spine fixture 7 is mounted on the moving table 62, the manual control adjuster 63 is a screw-type adjuster, and can realize precise displacement operation in the X-axis direction of the moving table 62, so that the horizontal movement of the spine fixing device 7 along the Z-axis can be realized through the horizontal screw rod 32, the horizontal movement of the spine fixing device 7 along the X axis can be realized by the moving table 62, so that the spinal cord impact can be realized on any surface position of the spine of the animal clamped on the spine fixing device 7.
The spine fixator 7 of this embodiment is a rat spine fixator, and as shown in fig. 8, it includes a fixator base 71 and a spine clamping jaw 72 provided on the fixator base, and a fixator screw hole 73 is provided on the bottom of the fixator base 71, and the fixator screw hole 73 matches with the screw hole on the mobile station 62 and is screwed and fixed by a bolt.
The control device 8 is provided with a touch panel 81, and the touch panel 81 can be used for setting relevant parameters of instruments such as the driving motor 9, the spinal cord impactor 4 and the distance measuring locator 5 and controlling the instruments, and specifically comprises the following steps: impact speed, impact depth, impact dwell time, precise control of the stage 6, moving the spinal cord to the impact head 41 in preparation for impact and initiation of impact, etc.
This embodiment is described below with reference to a specific experimental procedure:
a mouse with a specified standard is selected, firstly, the mouse is anesthetized, the hair on the back of the mouse is shaved off, the skin, the subcutaneous tissue, the muscle and the like of the corresponding part of the mouse vertebra T9-11 are sequentially cut along the central axis of the back by using a scalpel, the corresponding T10 vertebral plate is exposed, the mouse is fixed by using a spinal fixator 7 and is subjected to parallel laminectomy, the T10 vertebral plate is carefully bitten off by using rongeur, and the spinal cord is exposed. The mouse and the spine fixer 7 are placed and fixed on the object stage 6, the object stage 6 is controlled to move along the X axis by the fine-adjustment manual control adjuster 63, the object stage 6 is controlled to move along the Z axis of the horizontal moving device 3 by the touch panel 81, and the center of the spinal cord is moved to the lower part of the distance measuring positioner 5. The impact speed is set to 1 m/s by clicking the touch panel 81, the impact depths are set to 0.6 mm, and the impact dwell time is designed to be 500 ms. Clicking a preparation button of the touch panel 8112, automatically measuring the distance by the distance measuring positioner 5 according to the set impact depth, automatically controlling the vertical moving device 2 to move the cantilever 23, and moving the impact head 41 to a corresponding height; the horizontal movement device 3 is automatically controlled to move the part positioned by the distance measuring positioner 5 to the position right below the impact head 41, and the preparation is completed. At this time, the push rod 431 of the impact driving device 43 is manually pushed down, and it is confirmed again that the impact head 413 accurately corresponds to the impact portion. After the completion of the confirmation, the start button on the touch panel 81 is clicked, and after the countdown is finished, the impact head 41 automatically and vertically impacts the spinal cord and then resets, and the spinal cord injury is completed. Through automatic distance measurement and impact, the preparation process of the spinal cord injury impact model is accurate and objective, and the operation is simple and convenient.
In the description herein, references to the description of "an embodiment," "an example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. An automatic preparation device for a spinal cord injury animal model is characterized by comprising:
the operating platform comprises a base, a horizontal moving device and a vertical moving device, wherein the horizontal moving device is arranged on the upper surface of the base, the vertical moving device is vertical to the horizontal moving device, and driving motors are arranged on the horizontal moving device and the vertical moving device;
the spinal cord impactor is arranged on the vertical moving device and is used for impacting the spinal cord to form injury;
the distance measuring positioner is arranged on the vertical moving device and is used for positioning an impact part and automatically measuring the impact depth;
a spinal fixator mounted on the horizontal movement device for fixing the spine of the animal so as to expose and position the spinal cord;
and the control device is provided with a control panel and is used for setting relevant working parameters of the driving motor, the spinal cord impactor and the distance measuring positioner and controlling the driving motor, the spinal cord impactor and the distance measuring positioner to work.
2. The automated spinal cord injury animal model preparation device of claim 1, further comprising a stage mounted on the horizontal movement device and horizontally movable in a direction perpendicular to the movement direction of the horizontal movement device, the spinal fixator being mounted on the stage.
3. The apparatus for preparing an animal model of spinal cord injury according to claim 2, wherein a manual controller is disposed on the stage for manually controlling the horizontal movement of the stage relative to the horizontal moving device.
4. The automated spinal cord injury animal model preparation device of claim 1, wherein the vertical movement device comprises a vertical column, a vertical screw, and a cantilever, wherein,
the vertical upright post is fixedly connected to the operating platform;
the vertical screw is arranged on the side surface of the vertical upright column facing the direction of the horizontal moving device, one end part of the vertical screw is connected with the driving motor, and the driving motor drives the vertical screw to rotate;
the first end of cantilever is equipped with the screw thread through-hole, the screw thread through-hole with perpendicular screw rod connects soon, just the terminal surface of first end with the side of perpendicular stand is connected with sliding from top to bottom, the second end orientation of cantilever is on a parallel with horizontal migration device moving direction's direction level extends, the spinal cord impinger with the range finding locator is installed second end lower extreme.
5. The device for preparing an animal model of spinal cord injury automatically according to claim 1, wherein the spinal cord impactor comprises an impacting head, a sliding seat and an impacting driving device, the impacting head is detachably fixed on the lower surface of the sliding seat, the sliding seat is vertically slidably arranged on a cantilever of the vertical moving device, the impacting driving device is fixed on the cantilever, and the impacting driving device drives the sliding seat to vertically slide.
6. The automated spinal cord injury animal model preparation device of claim 5, wherein the impact driver comprises a push rod, a retraction spring, and an electromagnetic driver, wherein,
the electromagnetic driving part is fixed on the cantilever, and the push rod is connected with the electromagnetic driving part and drives the push rod to vertically move downwards through the electromagnetic driving part;
the lower end of the push rod is connected with the sliding seat;
the contraction spring is arranged at the upper end of the push rod and is extruded to store energy when the push rod vertically moves downwards, after the electromagnetic driving piece stops driving, the contraction spring releases energy to drive the push rod to vertically move upwards, and the sliding seat and the impact head synchronously reset upwards.
7. The automated spinal cord injury animal model preparation device of claim 1, wherein the horizontal movement device comprises a horizontal seat, a horizontal screw, and a slide, wherein,
the horizontal base body is fixedly connected to the upper end face of the operating platform;
the horizontal screw is arranged on the horizontal base body, one end part of the horizontal screw is connected with the driving motor, and the driving motor drives the horizontal screw to rotate;
the sliding seat is provided with a thread through hole, the thread through hole is connected with the horizontal screw rod in a screwing mode, the bottom surface of the sliding seat is connected with the bottom surface of the horizontal seat body in a horizontally sliding mode, and the spine fixator is installed on the top surface of the sliding seat.
8. The device for preparing an automatic animal model of spinal cord injury according to claim 1, wherein the distance measuring locator is a laser displacement sensor, the laser displacement sensor emits laser to the spinal fixation device, the position of laser irradiation is the position where the spinal cord impactor impacts the spinal cord, and the spinal fixation device is moved by operating the horizontal moving device to accurately position the impacted position of the spinal cord.
9. The automated spinal cord injury animal model creation device of claim 1, wherein relevant operating parameters of the spinal cord impactor include impact velocity, impact depth and impact dwell time.
10. The apparatus of claim 9, wherein the control panel is a touch panel, and after the touch panel sets the relevant working parameters of the spinal cord impactor and starts the apparatus, the distance measuring locator automatically measures the impact depth and then automatically adjusts the height of the spinal cord impactor, and after the adjustment, the spinal cord is automatically impacted.
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