CN115006288B - Isoak element vesicle and preparation method thereof - Google Patents
Isoak element vesicle and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an isoquercitrin vesicle and a preparation method thereof, which are characterized in that: the composite material consists of the following components in percentage by mass: 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 20-50% of PPG-13-decyl tetradecyl polyether-24%, 1-5% of PPG-24-glycereth-24 and 2-8% of water. The isoquercitrin vesicle prepared by the invention solves the dissolution problem of isoquercitrin, and has the beneficial effects of convenient use, good stability, good transdermal effect, obvious whitening, antiallergic and anti-wrinkle effects and the like.
Description
Technical Field
The invention relates to the technical field of cosmetic carrier preparation, in particular to an isoquercitrin vesicle and a preparation method thereof.
Background
With the development of economy and society, consumers are increasingly careful about the efficacy and safety of cosmetics, especially in the aspects of whitening, anti-aging and sensitive muscle care. The isoquercitrin is an active ingredient contained in natural plants, has good anti-inflammatory and antioxidant effects, and can be used for whitening, anti-aging and sensitive muscle products. The bioactive components of isoquercitrin include antioxidant, immunomodulating, antibacterial, antitumor, lipid metabolism regulating, neuroprotection, diabetes improving, myocardial protection, cardiovascular disease resisting, senile dementia preventing and treating etc. However, isoquercitrin has the problems of poor solubility, poor percutaneous absorption, low bioavailability and the like, and is difficult to apply in cosmetics. Therefore, the method capable of solving the solubility of the isoquercitrin and improving the bioavailability has wide market prospect.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the isoquercitrin vesicle which is convenient to use, good in stability and good in transdermal effect and the preparation method thereof. The preparation method has the advantages of simple preparation process, high efficiency and low preparation cost.
In order to solve the technical problems, the invention adopts the following technical scheme: an isoquercitrin vesicle, characterized in that: the composite material consists of the following components in percentage by mass: 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 20-50% of PPG-13-decyl tetradecyl polyether-24%, 1-5% of PPG-24-glycereth-24 and 2-8% of water.
The isoquercitrin, CAS number: 482-35-9, the number on the catalog of used cosmetic raw materials (2021 edition) is 07829, the purity is > 90%.
The PPG-13-decyl tetradecyl polyether-24 is assigned a number 00848 in the catalog of used cosmetic raw materials (2021).
The PPG-24-glycereth-24, CAS number 51258-15-2, was assigned a number 00887 on the catalog of used cosmetic raw materials (2021 edition).
The invention also provides a preparation method of the isoquercitrin vesicle, which is characterized by comprising the following steps:
A. weighing 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 20-50% of PPG-13-decyl tetradecyl polyether-24%, 1-5% of PPG-24-glycereth-24 and 2-8% of water according to the following mass percentages;
B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, and carrying out subcritical dissolution for 60-90 min at the temperature of 30-70 ℃;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycereth-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, and carrying out subcritical dissolution at 30-70 ℃ for 40-60 min to obtain the isoquercitrin vesicle with the particle size of 80-120 nm.
The subcritical equipment is a CBE-5L subcritical fluid extraction test device manufactured by Henan province subcritical biotechnology limited company.
The isoquercitrin vesicle adopts the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerol polyether-24 in specific proportion to carry out dissolution and encapsulation under subcritical conditions, and has the advantages of convenient use, good stability, good transdermal effect, whitening, anti-allergy and good anti-wrinkle effect.
The polarity of the subcritical water is enhanced, the solubility of the isoquercitrin in organic matters is improved, and in the subcritical water, the compound of PPG-13-decyl tetradecyl polyether-24 and PPG-24-glycereth-24 provides a polar kernel, so that the subcritical water-soluble isoquercitrin is more stably wrapped in the compound, and meanwhile, because two macromolecules of PPG-13-decyl tetradecyl polyether-24 and PPG-24-glycereth-24 are mutually entangled, the inclusion is more stable and has elasticity.
The invention has the beneficial effects that:
1) Solves the problem of dissolution of the isoquercitrin, and has the advantages of convenient use and good stability;
2) The transdermal effect is good;
3) The obtained isoquercitrin vesicle has excellent antiallergic, whitening and anti-wrinkle effects.
To demonstrate the beneficial effects of the present invention, the following experiments were performed.
The isoquercitrin vesicles were prepared according to the mass percentages of each substance shown in Table 1 and the preparation method described in example 1.
The components used in comparative example 1 were identical to those used in example 10, but were not prepared as described in example 1 of the present invention, and were simply stirred and mixed.
Comparative example 2 the preparation described in example 1 of the present invention was used, but without the addition of 1, 3-propanediol, the other ingredients were identical to example 10.
Comparative example 3 the preparation described in example 1 of the present invention was carried out, but without the addition of PPG-13-decyl tetradecyl polyether-24, the other components being identical to those of example 10.
Comparative example 4 the preparation described in example 1 of the present invention was used, but no PPG-24-glycereth-24 was added, and the other components were identical to those of example 10.
Comparative example 5 the preparation described in example 1 of the present invention was used, but no water was added, and the other components were identical to example 10.
Table 1 mass percentages of each component in examples and comparative examples and preparation method thereof
1. Stability test
The above samples were subjected to stability test at 5℃at 25℃at 45℃and at room temperature under light conditions, and the presence or absence of unstable phenomena such as precipitation and delamination was observed for 2 weeks, 4 weeks, 8 weeks and 12 weeks, and the stability test results are shown in Table 2.
TABLE 2 stability test results
O represents no delamination or no precipitation; and/indicates insolubilization.
From the test results shown in Table 2, the stability of the isoquercitrin vesicles prepared by the invention is tested for 12 weeks, all examples have good stability, no abnormal conditions such as precipitation and layering, and the comparative examples have the phenomena of insolubility, precipitation and layering, so that the isoquercitrin vesicles prepared by the invention have better illumination and high-low temperature stability.
Transdermal experiments
Selecting intact pig skin with equivalent thickness for transdermal test, cutting into square with side length of about 2.5 and cm, and placing between receiving tank and supply tank of Franz diffusion tank; in the receiving well was PBS buffer containing 5% tween 80 (ph=7.4); adding 2 mL of the reagent to be tested into the supply tank; the assembled Franz diffusion cell was placed in a transdermal diffusion device and experimental conditions were set as follows: the temperature was 37℃and the stirring speed was 400 rpm, and the transdermal time was 12 h.
After transdermal treatment, taking out the pigskin from the diffusion tank, wiping the residual sample on the surface of the pigskin with absorbent cotton, then sticking the first layer of skin on the surface of the pigskin with 3M adhesive tape, discarding, shearing the residual pigskin, placing the cut pigskin into a 50 mL centrifuge tube, adding 70% methanol 50 mL, carrying out ultrasonic treatment for 60min (400 w,40 Hz), filtering with a microporous filter membrane, taking 1 mL filtrate, placing the filtrate into a 5 mL volumetric flask, carrying out constant volume 5 mL with 70% methanol, shaking uniformly, filtering with a 0.45 mu M microporous filter membrane, and measuring the content of isoquercitrin by adopting an HPLC method to obtain the content of isoquercitrin in the skin.
Method for detecting isoquercitrin by HPLC, chromatographic conditions: chromatographic column: thermosyncronis C18 (250 mm ×4.6mm,5 μm); mobile phase: acetonitrile (a) -0.1% formic acid water (B); gradient elution: 0-5 min,12% (B); 5-15 min,22% (B); 15-25 min,28% (B); 25-30 min,30% (B); 30-40 min,35% (B); flow rate: 1 mL/min; the column temperature is 35 ℃; the detection wavelength is 370 nm; the sample loading was 20. Mu.L.
TABLE 3 transdermal test results (content of isoquercitrin in skin)
As can be seen from Table 3, the transdermal contents of isoquercitrin in examples 1-12 were all higher, while those in comparative examples 1-5 were all very low, with little. Transdermal content of the isoquercitrin vesicles of examples 1-12About 100-500 times that of comparative examples 1-5, up to 2610.19. Mu.g/cm 2 The method has the advantages that the isoquercitrin vesicle preparation technology is adopted, so that the percutaneous absorption of the isoquercitrin can be effectively promoted, and the isoquercitrin has better biological utilization.
Mildness test
60 volunteers aged 18-60 years were selected and tested for mildness by reference to the cosmetic safety Specification 2015 edition, the human skin patch test. Subject exclusion conditions are in accordance with GB 171492-1997 diagnostic criteria and treatment principles for cosmetic contact dermatitis, no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, no allergic history on skin care cosmetics, no systemic use of hormone drugs and immunosuppressants within 1 month, no pregnancy or lactation period, no ethical contraindications.
Table 4 mildness test sample cream composition
Examples 1-12 were added to the creams at 0.5% addition, respectively, and the cream formulations were as shown in Table 4. Adding the sample into a patch tester, applying the patch tester to the back of a subject by using a non-stimulated adhesive tape for 24 hours, and simultaneously applying a blank of the patch tester as a blank control; after removing the subject plaque tester, one skin reaction was observed at 0.5h,24h and 48h, respectively. The reaction results were recorded according to a grading standard, with no tingling sensation, mild tingling sensation, moderate tingling sensation, and the standard for evaluating mildness, as the test results are shown in table 5.
TABLE 5 results of mildness test experiments
As is clear from Table 5, in the patch experiments conducted for examples 1 to 12, the subjects did not have adverse reactions, which indicates that the quercitin vesicles prepared by supercritical have the characteristic of being mild.
4. Human efficacy testing
48 female volunteers with 18-55 years of age, sensitive muscles and wrinkles at the corners of eyes, which are not pregnant, breast-feeding and gestation were selected according to the voluntary principle, and were randomly divided into an experimental group and a control group, wherein the experimental group uses the face cream of the example 10 added in proportion after face cleaning in the morning and evening, the control group uses the face cream without the isoquercitrin vesicle (the usage amount is kept consistent, about 0.5 g/time), other skin care products are not used in the period, and the experimental requirement is that the volunteer test period is mainly indoor activity and long-time unprotected exposure to sunlight is avoided. Selecting faces and canthus of volunteers as test parts, respectively testing after cleaning faces of the volunteers after 28 days before use, and testing skin melanin content of a subject by using an MX18 melanin tester of German CK company, wherein the lower the measured value is, the lower the skin melanin content is; the skin moisture tester Glossemeter GL 200 was used to measure skin gloss values, the higher the measurement value, the higher the skin gloss was; detecting heme (ultra-high concentration) and the numerical value of an affected area by using a Miravex ANTERA 3D skin image analyzer, wherein the higher the measured value is, the higher the heme content is and the larger the affected area is; the number of wrinkles and the average depth of total wrinkles were measured using a PROMIS LITE wrinkle measuring instrument from LMI Technologies, germany, and the results of the human efficacy test are shown in Table 6.
TABLE 6 human efficacy test results
As can be seen from table 6, the experimenters of the experimental group showed a significant decrease in melanin content in the skin of 21.58% and an increase in skin glossiness of 22.98% after continuous use for 28 days; the improvement rate of the sensitive myocutaneous heme is 95.17%, and the influence area is also obviously reduced by 96.87%; the number of wrinkles decreased by 20.32% and the average depth of total wrinkles decreased by 20.42%. The face cream of the control group of the isoquercitrin vesicle prepared by the invention has no obvious improvement effect on melanin content, skin glossiness, heme influence area, number of wrinkles and average depth of total wrinkles, so that the isoquercitrin vesicle prepared by the invention has good whitening, antiallergic and anti-wrinkle effects.
In conclusion, the isoquercitrin vesicle prepared by the invention has the beneficial effects of convenient use, good stability, good transdermal effect and remarkable whitening, antiallergic and anti-wrinkle effects.
Detailed Description
Example 1:
a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 2% of isoquercitrin, 70% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycerin polyether-24% and 2% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolution time to be 60 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 60 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 92.4 nm.
Example 2: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 4% of isoquercitrin, 70% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 2% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃ and setting the subcritical dissolution time to be 90 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 86.5 nm.
Example 3: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 6% of isoquercitrin, 70% of 1, 3-propylene glycol, 20% of PPG-13-decyl tetradecyl polyether-24%, 2% of PPG-24-glycereth-24 and 2% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 70 ℃ and setting the subcritical dissolution time to be 70min;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 70 ℃, and carrying out subcritical dissolution for 40 minutes to obtain the isoquercitrin vesicle with the average particle size of 105.7 and nm.
Example 4: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 68% of 1, 3-propylene glycol, 20% of PPG-13-decyl tetradecyl polyether-24%, 2% of PPG-24-glycereth-24 and 2% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 30 ℃ and setting the subcritical dissolution time to be 90 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 30 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 112.4 nm.
Example 5: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 2% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 48% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolution time to be 60 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 109.1 and nm.
Example 6: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 4% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 4% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃ and setting the subcritical dissolution time to be 90 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 107.0 nm.
Example 7: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 6% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 4% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.7MPa, setting the temperature to be 40 ℃, and setting the subcritical dissolution time to be 80 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.7MPa, setting the temperature to be 40 ℃, and carrying out subcritical dissolution for 50min to obtain the isoquercitrin vesicle with the average particle size of 93.7 and nm.
Example 8: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 4% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 50 ℃ and setting the subcritical dissolution time to be 70min;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 50 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 105.5 and nm.
Example 9: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 4% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolution time to be 80 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 60 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 113.4 nm.
Example 10: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 6% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolution time to be 70min;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and carrying out subcritical dissolution for 50min to obtain the isoquercitrin vesicle with the average particle size of 101.6 nm.
Example 11: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether-24%, 24% of PPG-24-glycereth-24% and 6% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 30 ℃ and setting the subcritical dissolution time to be 70min;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 40 ℃, and carrying out subcritical dissolution for 40 minutes to obtain the isoquercitrin vesicle with the average particle size of 114.2 and nm.
Example 12: a method for preparing isoquercitrin vesicles is characterized by comprising the following steps:
A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 24% of PPG-13-decyl tetradecyl polyether, 24% of PPG-24-glycereth-24% and 8% of water according to the following mass percentages;
B. adding the isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolution time to be 80 minutes;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 60 ℃, and carrying out subcritical dissolution for 60 minutes to obtain the isoquercitrin vesicle with the average particle size of 94.8 nm.
Claims (1)
1. An isoquercitrin vesicle, characterized in that: the preparation method comprises the following steps:
A. weighing 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 20-50% of PPG-13-decyl tetradecyl polyether-24%, 1-5% of PPG-24-glycereth-24 and 2-8% of water according to the following mass percentages;
B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, and dissolving at 30-70 ℃ for 60-90 min;
C. and C, adding the PPG-13-decyl tetradecyl polyether-24 and the PPG-24-glycerin polyether-24 weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, and dissolving at 30-70 ℃ for 40-60 min to obtain the isoquercitrin vesicle with the particle size of 80-120 nm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113244130A (en) * | 2021-04-30 | 2021-08-13 | 苏州纳康生物科技有限公司 | Ultra-strong oxidation-resistant deformable vesicle and preparation method and application thereof |
| CN114159316A (en) * | 2021-11-24 | 2022-03-11 | 珀莱雅化妆品股份有限公司 | Microcapsule liposome for caring skin around eyes and preparation method thereof |
| CN114224788A (en) * | 2021-12-23 | 2022-03-25 | 珀莱雅化妆品股份有限公司 | Anti-wrinkle double microcapsule and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113244130A (en) * | 2021-04-30 | 2021-08-13 | 苏州纳康生物科技有限公司 | Ultra-strong oxidation-resistant deformable vesicle and preparation method and application thereof |
| CN114159316A (en) * | 2021-11-24 | 2022-03-11 | 珀莱雅化妆品股份有限公司 | Microcapsule liposome for caring skin around eyes and preparation method thereof |
| CN114224788A (en) * | 2021-12-23 | 2022-03-25 | 珀莱雅化妆品股份有限公司 | Anti-wrinkle double microcapsule and preparation method thereof |
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| "亚临界-水萃取甘草抗氧化物质的研究";樊蕊等;《食品科技》;第第39卷卷(第第2期期);192-197 * |
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