CN115006288A - Isoquercitrin vesicle and preparation method thereof - Google Patents

Abstract

The invention relates to isoquercitrin vesicles and a preparation method thereof, and is characterized in that: the composite material comprises the following components in percentage by mass: 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 0-50% of PPG-13-decyltetradecanol polyether-2420%, 241-5% of PPG-24-glyceryl polyether-241% and 2-8% of water. The isoquercitrin vesicle prepared by the invention solves the problem of dissolving isoquercitrin, and has the beneficial effects of convenient use, good stability, good transdermal effect, obvious whitening, anti-allergy and anti-wrinkle effects and the like.

Description

Isoquercitrin vesicle and preparation method thereof

Technical Field

The invention relates to the technical field of preparation of cosmetic carriers, and particularly relates to isoquercitrin vesicles and a preparation method thereof.

Background

With the development of economic society, consumers are more and more interested in the efficacy and safety of cosmetics, especially in whitening, anti-aging and sensitive muscle care. Isoquercitrin is an active ingredient contained in natural plants, has good anti-inflammatory and antioxidant effects, and can be used for whitening, anti-aging and sensitive skin products. The biological activities of isoquercitrin include antioxidation, immunoregulation, anti-bacteria, anti-tumor, lipid metabolism regulation, neuroprotection, diabetes improvement, myocardial protection, cardiovascular disease resistance and senile dementia prevention and treatment. However, isoquercitrin has problems of poor solubility, poor percutaneous absorption, low bioavailability, etc., and is difficult to apply in cosmetics. Therefore, the method for solving the problem of solubility of isoquercitrin and improving the bioavailability has wide market prospect.

Disclosure of Invention

The technical problem to be solved by the invention is to provide the isoquercitrin vesicle which is convenient to use, good in stability and good in transdermal effect and the preparation method thereof. The method has the advantages of simple preparation process, high efficiency and low preparation cost.

In order to solve the technical problem, the invention adopts the following technical scheme: an isoquercitrin vesicle, comprising: the composite material comprises the following components in percentage by mass: 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 0-50% of PPG-13-decyltetradecanol polyether-2420%, 241-5% of PPG-24-glyceryl polyether-241% and 2-8% of water.

The isoquercitrin, CAS number: 482-35-9, number 07829 on the catalogue of used cosmetic raw materials (2021 edition), purity > 90%.

PPG-13-decyltetradecanol polyether-24 was assigned the number 00848 on the catalogue of used cosmetic raw materials (2021 edition).

The PPG-24-glyceryl polyether-24, CAS number 51258-15-2, on the catalogue of used cosmetic raw materials (2021 edition) is 00887.

The invention also provides a preparation method of the isoquercitrin vesicle, which is characterized by comprising the following steps of:

A. weighing 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 0-50% of PPG-13-decyltetradecanol polyether-2420%, 241-5% of PPG-24-glyceryl polyether and 2-8% of water according to the following mass percentages;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, setting the temperature to be 30-70 ℃, and carrying out subcritical dissolution for 60-90 min;

C. and (B) adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 weighed in the step (A) into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, setting the temperature to be 30-70 ℃, and performing subcritical dissolution for 40-60 min to obtain isoquercitrin vesicles with the particle size of 80-120 nm.

The subcritical equipment is a CBE-5L subcritical fluid extraction test device produced by subcritical biotechnology limited in Henan province.

The isoquercitrin vesicle adopts PPG-13-decyltetradecanol polyether-24 and PPG-24-glyceryl polyether-24 in a specific proportion, is dissolved and coated under a subcritical condition, and has the advantages of convenience in use, good stability, good transdermal effect, and good whitening, anti-allergy and anti-wrinkle effects.

The polarity of water in a subcritical state is enhanced, the solubility of water to organic matters is improved, isoquercitrin can be dissolved in the subcritical state, PPG-13-decyltetradecyltetradecyltetradecyltetradecyltetradecyltetradecyltetradecyltetradecyltetradecylty-24 and PPG-24-glycerolpolyether-24 are compounded to provide a polar inner core, so that isoquercitrin dissolved in subcritical water can be wrapped in the water more stably, and simultaneously, the wrapping body is more stable and elastic because two macromolecules of PPG-13-decyltetradecyltetradecyltetradecyltetradecyltetradecyltetradecylty-24 and PPG-24-glycerolpolyether-24 are twisted mutually.

The invention has the beneficial effects that:

1) solves the problem of the dissolution of isoquercitrin, and has the advantages of convenient use and good stability;

2) the transdermal effect is good;

3) the obtained isoquercitrin vesicle has excellent anti-allergy, whitening and anti-wrinkle effects.

To demonstrate the beneficial effects of the present invention, the following experiments were performed.

Isoquercitrin vesicles were prepared according to the mass percentages of the substances shown in table 1 and the preparation method described in example 1.

Comparative example 1 used the same components as in example 10, but without the preparation described in inventive example 1, mixed with simple stirring.

Comparative example 2 the procedure of the invention described in example 1 was followed, but without the addition of 1, 3-propanediol, and the other ingredients were identical to those of example 10.

Comparative example 3 the procedure of the invention as described in example 1 was followed, but PPG-13-decyltetradecanol polyether-24 was not added, and the other components were identical to those of example 10.

Comparative example 4 the procedure of the invention described in example 1 was followed, but PPG-24-glyceryl polyether-24 was not added, and the other components were identical to those of example 10.

Comparative example 5 the procedure of the invention described in example 1 was followed, but no water was added and the other ingredients were identical to those of example 10.

Table 1 mass percentages of components and methods of preparation in examples and comparative examples

1. Stability test

The samples are respectively placed under the illumination conditions of 5 ℃, 25 ℃, 45 ℃ and room temperature for stability experiments, whether the samples have unstable phenomena such as precipitation, delamination and the like is observed for 2 weeks, 4 weeks, 8 weeks and 12 weeks, and the stability test results are shown in table 2.

Table 2 stability test results

O means no delamination and no precipitation; and/means not dissolved.

From the test results shown in table 2, after the stability test of 12 weeks, all the examples have better stability, and no abnormal conditions such as precipitation and delamination exist, while the comparative examples have phenomena such as insolubilization, precipitation and delamination, and therefore, the isoquercitrin vesicles prepared by the invention have better illumination and high and low temperature stability.

Transdermal test

Selecting intact pigskin with equivalent thickness to carry out a transdermal experiment, cutting the intact pigskin into a square with the side length of about 2.5 cm after treatment, and placing the square between a receiving pool and a supply pool of a Franz diffusion pool; PBS buffer containing 5% Tween 80 (pH = 7.4) in the receiving cell; adding 2 mL of a test agent into the supply pool; the assembled Franz diffusion cell was placed in a transdermal diffusion apparatus and the experimental conditions were set as follows: the temperature was 37 ℃, the stirring speed was 400 rpm and the skin penetration time was 12 h.

And after the skin penetration is finished, taking out the pigskin from the diffusion pool, wiping a sample remained on the surface of the pigskin with absorbent cotton, sticking a first layer of skin on the surface of the pigskin with a 3M adhesive tape and discarding, cutting the rest pigskin into pieces, putting the cut pigskin into a 50 mL centrifuge tube, adding 50 mL of 70% methanol, performing ultrasonic treatment for 60min (400 w and 40 Hz), filtering with a microporous membrane, taking 1 mL of filtrate, putting the filtrate into a 5 mL volumetric flask, putting the 70% methanol into 5 mL, shaking up the filtrate, filtering with a 0.45 mu M microporous membrane, and measuring the content of isoquercitrin by using an HPLC method, namely the content of isoquercitrin in the skin.

The HPLC detection method of isoquercitrin has the following chromatographic conditions: and (3) chromatographic column: thermosyncronis C18(250 mm. times.4.6 mm, 5 μm); mobile phase: acetonitrile (a) -0.1% formic acid water (B); gradient elution: 0-5 min, 12% (B); 5-15 min, 22% (B); 15-25 min, 28% (B); 25-30 min, 30% (B); 30-40 min, 35% (B); flow rate: 1 mL/min; the column temperature was 35 ℃; the detection wavelength is 370 nm; the amount of sample was 20. mu.L.

TABLE 3 transdermal test results (Isoquercitrin content in skin)

As can be seen from Table 3, the transdermal contents of isoquercitrin of examples 1-12 were all high, whereas those of comparative examples 1-5 were all very low and almost none. The transdermal content of the isoquercitrin vesicles in the examples 1-12 is about 500 times that of the isoquercitrin vesicles in the comparative examples 1-5, and the transdermal content can reach 2610.19 mu g/cm ² The patent shows that the isoquercitrin vesicle preparation technology can effectively promote the percutaneous absorption of isoquercitrin, so that the isoquercitrin has better biological utilization.

Mild test

60 volunteers aged 18-60 years were selected and subjected to a mild test according to the cosmetic safety technical Specification 2015 edition-human skin Patch test. The exclusion conditions of the subjects accord with GB 171492-1997 diagnosis standard and treatment principle of cosmetic contact dermatitis, no serious systemic diseases, no immunodeficiency or autoimmune diseases, no active allergic diseases, no allergic history to skin care cosmetics, no systemic use of hormones and immunosuppressants in 1 month, no pregnancy or lactation period and no ethical contraindication.

TABLE 4 Mild test sample cream composition

Examples 1-12 were each added to a cream at an add-on level of 0.5% and the cream formulation composition is shown in table 4. Adding the sample into a spot tester, and applying the sample to the back of a subject for 24 hours by using a non-irritant adhesive tape, wherein a blank of the spot tester is applied as a blank control; after the test substance spot tester is removed, the skin reaction is observed for one time at 0.5h, 24h and 48h respectively. The reaction results were recorded according to the grading standards, with no stinging sensation at grade 0, almost no stinging sensation at grade 1, mild stinging sensation at grade 2, moderate stinging sensation at grade 3, and severe stinging sensation at grade 4, as standards for the evaluation of mildness, and the experimental results are shown in table 5.

TABLE 5 Mild Property test results

As can be seen from Table 5, in the patch experiments performed in examples 1 to 12, the subjects had no adverse reactions, which indicates that the isoquercitrin vesicles prepared by supercritical method have good mildness.

4. Human body efficacy testing

48 female volunteers with age of 18-55 years, sensitive muscles and wrinkles at canthus, non-preparation pregnancy, lactation and pregnancy are selected according to a voluntary principle and randomly divided into an experimental group and a control group, the experimental group is added with the cream of the embodiment 10 according to the proportion after face cleaning in the morning and in the evening, the control group uses the cream without isoquercitrin vesicles (the usage amount is kept consistent and is about 0.5 g/time) continuously for 28 days, other skin care products are not used in the period, and the experiment requires that the volunteers mainly move indoors during the test period, so that the long-time unprotected exposure to sunlight is avoided. Selecting the face and the canthus of the volunteer as testing parts, respectively before use, after using for 28 days, testing after cleaning the face of the volunteer, and using MX18 melanin tester of CK company in Germany to test the skin melanin content of the volunteer, wherein the lower the measured value is, the less the skin melanin content is; measuring the skin gloss value by using a skin moisture tester Glossimeter GL 200, wherein the higher the measured value is, the higher the skin gloss is indicated; detecting values of heme (ultrahigh concentration) and an affected area by using a Miravex ANTERA 3D skin image analyzer, wherein the higher the measured value is, the higher the heme content is and the larger the affected area is; the number of wrinkles and the average depth of total wrinkles were measured using a PROMIS LITE wrinkle Meter, LMI Technologies, Germany, and the results of the human efficacy test are shown in Table 6.

Table 6 human efficacy test results

As can be seen from table 6, the content of melanin in the skin of the experimenters in the experimental group is significantly reduced by 21.58% and the skin glossiness is improved by 22.98% after the experimenters continue to use the skin for 28 days; the improvement rate of sensitive skin heme is 95.17%, and the influence area is also obviously reduced by 96.87%; the number of wrinkles decreased by 20.32%, and the average depth of the total wrinkles also decreased by 20.42%. The control group of the cream without the isoquercitrin vesicle prepared by the invention has no obvious improvement effect on melanin content, skin glossiness, heme and heme affected area, wrinkle quantity and total wrinkle average depth, so the isoquercitrin vesicle prepared by the invention has better whitening, anti-allergy and anti-wrinkle effects.

In conclusion, the isoquercitrin vesicle prepared by the invention has the beneficial effects of convenience in use, good stability, good transdermal effect and remarkable whitening, anti-allergy and anti-wrinkle effects.

Detailed Description

Example 1:

an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 2% of isoquercitrin, 70% of 1, 3-propylene glycol, 13-2424% of PPG-13-decyltetradecanol polyether, 242% of PPG-24-glyceryl polyether and 2% of water according to the following mass percentage;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 60 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 60min to obtain the isoquercitrin vesicles with the average particle size of 92.4 nm.

Example 2: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 4% of isoquercitrin, 70% of 1, 3-propylene glycol, 13% of PPG-13-decyltetradecanol polyether-2422%, 24% of PPG-glyceryl polyether-242% and 2% of water according to the following mass percentage;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 90 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 86.5 nm.

Example 3: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 6% of isoquercitrin, 70% of 1, 3-propylene glycol, 13% of PPG-13-decyltetradecanol polyether-2420%, 24% of PPG-glyceryl polyether-242% and 2% of water according to the following mass percentage;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 70 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 40min to obtain isoquercitrin vesicles with the average particle size of 105.7 nm.

Example 4: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 68% of 1, 3-propylene glycol, 13-2420% of PPG-13-decyltetradecanol polyether, 242% of PPG-24-glyceryl polyether and 2% of water according to the following mass percentage;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 30 ℃, and setting the subcritical dissolving time to be 90 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 30 ℃, and setting the subcritical dissolving time to be 60min to obtain the isoquercitrin vesicles with the average particle size of 112.4 nm.

Example 5: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 2% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyltetradecanol polyether, 2450% of PPG-24-glyceryl polyether and 48% of water according to the following mass percentages;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 60 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 109.1 nm.

Example 6: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 4% of isoquercitrin, 40% of 1, 3-propylene glycol, 2448% of PPG-13-decyltetradecanol polyether, 244% of PPG-24-glycerol polyether and 4% of water according to the following mass percentages;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 90 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 70 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 107.0 nm.

Example 7: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 6% of isoquercitrin, 40% of 1, 3-propylene glycol, 24% of PPG-13-decyltetradecanol polyether, 244% of PPG-24-glycerol polyether and 4% of water according to the following mass percentages;

B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.7MPa, setting the temperature to be 40 ℃, and setting the subcritical dissolving time to be 80 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.7MPa, setting the temperature to be 40 ℃, and setting the subcritical dissolving time to be 50min to obtain isoquercitrin vesicles with the average particle size of 93.7 nm.

Example 8: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 40% of 1, 3-propylene glycol, 2444% of PPG-13-decyltetradecanol polyether, 244% of PPG-24-glycerol polyether and 4% of water according to the following mass percentages;

B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 70 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 105.5 nm.

Example 9: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 50% of PPG-13-decyltetradecanol polyether, 2434% of PPG-24-glyceryl polyether and 4% of water according to the following mass percentages;

B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 80 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 1.0MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 113.4 nm.

Example 10: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 2432% of PPG-13-decyltetradecanol polyether, 244% of PPG-24-glycerol polyether and 6% of water according to the following mass percentages;

B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 70 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6MPa, setting the temperature to be 50 ℃, and setting the subcritical dissolving time to be 50min to obtain isoquercitrin vesicles with the average particle size of 101.6 nm.

Example 11: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 50% of PPG-13-decyltetradecanol polyether, 2434% of PPG-24-glyceryl polyether and 6% of water according to the following mass percentage;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 30 ℃, and setting the subcritical dissolving time to be 70 min;

C. and (B) adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 weighed in the step (A) into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.8MPa, setting the temperature to be 40 ℃, and setting the subcritical dissolving time to be 40min to obtain the isoquercitrin vesicles with the average particle size of 114.2 nm.

Example 12: an isoquercitrin vesicle and a preparation method thereof are characterized by comprising the following steps:

A. weighing 8% of isoquercitrin, 50% of 1, 3-propylene glycol, 2430% of PPG-13-decyltetradecanol polyether, 244% of PPG-24-glyceryl polyether and 8% of water in percentage by mass;

B. adding the isoquercitrin, the 1, 3-propylene glycol and the water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 80 min;

C. and D, adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 which are weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.9MPa, setting the temperature to be 60 ℃, and setting the subcritical dissolving time to be 60min to obtain isoquercitrin vesicles with the average particle size of 94.8 nm.

Claims (2)

1. An isoquercitrin vesicle, comprising: the composite material consists of the following components in percentage by mass: 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 0-50% of PPG-13-decyltetradecanol polyether-2420%, 241-5% of PPG-24-glyceryl polyether-241% and 2-8% of water.

2. A preparation method of isoquercitrin vesicles is characterized by comprising the following steps:

A. weighing 2-8% of isoquercitrin, 40-70% of 1, 3-propylene glycol, 0-50% of PPG-13-decyltetradecanol polyether-2420%, 241-5% of PPG-24-glyceryl polyether and 2-8% of water according to the following mass percentages;

B. adding isoquercitrin, 1, 3-propylene glycol and water weighed in the step A into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, setting the temperature to be 30-70 ℃, and carrying out subcritical dissolution for 60-90 min;

C. and (B) adding the PPG-13-decyltetradecanol polyether-24 and the PPG-24-glyceryl polyether-24 weighed in the step (A) into a reaction kettle of subcritical equipment, setting the pressure in the kettle to be 0.6-1.0 MPa, setting the temperature to be 30-70 ℃, and performing subcritical dissolution for 40-60 min to obtain isoquercitrin vesicles with the particle size of 80-120 nm.