CN115005237B - Tebuconazole compound sterilization composition and application thereof - Google Patents
Tebuconazole compound sterilization composition and application thereof Download PDFInfo
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- 239000005839 Tebuconazole Substances 0.000 title claims abstract description 50
- 239000000203 mixture Substances 0.000 title claims abstract description 29
- -1 Tebuconazole compound Chemical class 0.000 title claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 8
- 239000003814 drug Substances 0.000 claims abstract description 73
- 239000000284 extract Substances 0.000 claims abstract description 50
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 claims abstract description 35
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 20
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
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- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
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- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 235000010674 Prunella vulgaris Nutrition 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
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- 238000010790 dilution Methods 0.000 description 1
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- 230000008029 eradication Effects 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides a tebuconazole compound bactericidal composition and application thereof, and belongs to the technical field of bactericides. The invention provides a tebuconazole compound sterilization composition which is characterized in that the mass ratio of tebuconazole to traditional Chinese medicine extract in the sterilization composition is 1-3:20-30; the traditional Chinese medicine extract is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of selfheal, 5-15 parts of lithospermum, and 5-15 parts of clove. The invention provides a tebuconazole compound sterilization composition which is prepared by compounding tebuconazole and traditional Chinese medicine extracts, and has a strong inhibition effect on sheath blight bacteria of wheat, and the prevention effect on the sheath blight disease of wheat in the field can reach more than 97%.
Description
Technical Field
The invention relates to the technical field of bactericides, in particular to a tebuconazole compound bactericidal composition and application thereof.
Background
Tebuconazole is an organic compound with molecular formula of C 16 H 22 ClN 3 O is a high-efficiency broad-spectrum systemic triazole bactericidal pesticide, has three functions of protection, treatment and eradication, and has wide bactericidal spectrum and long lasting period. Like all triazole fungicides, tebuconazole inhibits the biology of ergosterol in fungiAnd (5) synthesizing. But the tebuconazole has low toxicity, and people contact with the tebuconazole at high concentration or contact with the tebuconazole for a long time or cause poisoning, so that the toxic effect of the tebuconazole is reduced or the use concentration and the application amount of the tebuconazole are reduced, and the personal safety of agricultural operators and farmers is ensured. However, how to ensure the sterilization effect of tebuconazole on the basis of reducing the use concentration or the application amount of tebuconazole is a big problem.
Disclosure of Invention
The invention aims to provide a tebuconazole compound bactericidal composition and application thereof, which can reduce the use concentration of tebuconazole and improve the inhibition effect on wheat sheath blight.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a tebuconazole compound bactericidal composition, wherein the mass ratio of tebuconazole to traditional Chinese medicine extracts in the bactericidal composition is 1-3:20-30;
the traditional Chinese medicine extract is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of selfheal, 5-15 parts of lithospermum, and 5-15 parts of clove.
Preferably, the preparation method of the traditional Chinese medicine extract comprises the following steps:
(1) Drying Prunellae Spica, radix Arnebiae, and flos Caryophylli, and pulverizing to obtain Chinese medicinal powder;
(2) Mixing the traditional Chinese medicine raw material powder with an ethanol solution, and performing ultrasonic extraction to obtain a traditional Chinese medicine extract;
(3) Concentrating the traditional Chinese medicine extract to obtain the traditional Chinese medicine extract.
Preferably, the temperature of the drying is 40-60 ℃, and the time of the drying is 1-3 hours.
Preferably, the particle size of the pulverization is 100 to 200 mesh.
Preferably, the mass volume ratio of the traditional Chinese medicine raw material powder to the ethanol solution is 1g: 10-15 ml.
Preferably, the volume concentration of the ethanol solution is 70-90%.
Preferably, the power of ultrasonic extraction is 200-350W, and the time is 20-40 min.
Preferably, the concentration is heating concentration, the temperature of the heating concentration is 60-80 ℃, and the time of the heating concentration is 30-60 min.
The invention also provides application of the tebuconazole compound bactericidal composition in preparation of a medicament for preventing and controlling rhizoctonia cerealis.
The invention provides a tebuconazole compound sterilization composition which is prepared by compounding tebuconazole and traditional Chinese medicine extracts, and has a strong inhibition effect on sheath blight bacteria of wheat, and the prevention effect on the sheath blight disease of wheat in the field can reach more than 97%.
Detailed Description
The invention provides a tebuconazole compound bactericidal composition, wherein the mass ratio of tebuconazole to traditional Chinese medicine extracts in the bactericidal composition is 1-3:20-30;
the traditional Chinese medicine extract is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of selfheal, 5-15 parts of lithospermum, and 5-15 parts of clove.
In the invention, the mass ratio of the tebuconazole to the traditional Chinese medicine extract is preferably 2:25.
In the invention, the traditional Chinese medicine extract is preferably prepared from the following traditional Chinese medicine raw materials in parts by weight: 8-12 parts of selfheal, 8-12 parts of lithospermum, 8-12 parts of clove, and more preferably 10 parts of selfheal, 10 parts of lithospermum and 10 parts of clove.
In the invention, the preparation method of the traditional Chinese medicine extract comprises the following steps:
(1) Drying Prunellae Spica, radix Arnebiae, and flos Caryophylli, and pulverizing to obtain Chinese medicinal powder;
(2) Mixing the traditional Chinese medicine raw material powder with an ethanol solution, and performing ultrasonic extraction to obtain a traditional Chinese medicine extract;
(3) Concentrating the traditional Chinese medicine extract to obtain the traditional Chinese medicine extract.
The invention firstly takes selfheal, lithospermum and clove to dry and crush, and the traditional Chinese medicine raw material powder is obtained.
In the present invention, the temperature of the drying is preferably 40 to 60 ℃, more preferably 45 to 55 ℃, still more preferably 50 ℃; the drying time is preferably 1 to 3 hours, more preferably 2 hours.
In the present invention, the particle size of the pulverization is preferably 100 to 200 mesh, more preferably 120 to 180 mesh, still more preferably 150 mesh.
After the traditional Chinese medicine raw material powder is prepared, the traditional Chinese medicine raw material powder is mixed with ethanol solution, and ultrasonic extraction is carried out to obtain traditional Chinese medicine extract.
In the invention, the mass volume ratio of the traditional Chinese medicine raw material powder to the ethanol solution is preferably 1g:10 to 15ml, more preferably 1g:12ml.
In the present invention, the volume concentration of the ethanol solution is preferably 70 to 90%, more preferably 80%.
In the present invention, the power of the ultrasonic extraction is preferably 200 to 350W, more preferably 250 to 300W, still more preferably 280W; the time of the ultrasonic wave is preferably 20 to 40 minutes, more preferably 25 to 35 minutes, still more preferably 30 minutes.
In the present invention, after ultrasonic extraction, solid-liquid separation by centrifugation is preferably employed.
In the present invention, the rotational speed of the centrifugation is preferably 500 to 1000rpm, more preferably 800rpm; the time for the centrifugation is preferably 10 to 30 minutes, more preferably 20 minutes.
After the traditional Chinese medicine extract is prepared, the traditional Chinese medicine extract is concentrated to obtain the traditional Chinese medicine extract.
In the present invention, the concentration is preferably concentration by heating.
In the present invention, the temperature of the heat concentration is preferably 60 to 80 ℃, more preferably 65 to 75 ℃, still more preferably 70 ℃; the time for the heating and concentrating is preferably 30 to 60 minutes, more preferably 40 to 50 minutes, and still more preferably 45 minutes.
In the invention, the solid traditional Chinese medicine extract is obtained after heating and concentrating.
In the invention, the traditional Chinese medicine extract is also subjected to crushing treatment. The crushed material is preferably sieved with a 400-800 mesh sieve, more preferably 460-650 mesh sieve, and even more preferably 540 mesh sieve.
The invention also provides application of the tebuconazole compound bactericidal composition in preparation of a medicament for preventing and controlling rhizoctonia cerealis.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Weighing 10 parts of selfheal, 10 parts of lithospermum and 10 parts of clove, placing in an oven, drying at 50 ℃ for 2 hours, taking out, crushing, and sieving with a 150-mesh sieve to obtain traditional Chinese medicine raw material powder. Mixing the traditional Chinese medicine raw material powder with 80% ethanol solution according to the mass volume ratio of 1g: mixing 12ml, ultrasonic extracting at power 280W for 30min, taking out, and centrifuging at 800rpm for 20min to obtain Chinese medicinal extractive solution. Concentrating the Chinese medicinal extractive solution at 70deg.C for 45min, pulverizing the concentrated solid, sieving with 540 mesh sieve, and collecting the undersize to obtain Chinese medicinal extract.
Example 2
Weighing 10 parts of selfheal, 12 parts of lithospermum and 15 parts of clove, placing in an oven, drying at 40 ℃ for 3 hours, taking out, crushing, and sieving with a 100-mesh sieve to obtain traditional Chinese medicine raw material powder. Mixing the traditional Chinese medicine raw material powder with 70% ethanol solution according to the mass volume ratio of 1g: mixing 15ml, ultrasonic extracting at power of 350W for 20min, taking out, and centrifuging at 500rpm for 30min to obtain Chinese medicinal extractive solution. Concentrating the Chinese medicinal extractive solution at 60deg.C for 60min, pulverizing the concentrated solid, sieving with 400 mesh sieve, and collecting the undersize to obtain Chinese medicinal extract.
Example 3
15 parts of selfheal, 12 parts of lithospermum and 10 parts of clove are weighed, placed in an oven, dried for 21 hours at 60 ℃, taken out, crushed and sieved by a 200-mesh sieve, and the traditional Chinese medicine raw material powder is obtained. Mixing the traditional Chinese medicine raw material powder with 90% ethanol solution according to the mass volume ratio of 1g: mixing 10ml, ultrasonic extracting at power of 200W for 40min, taking out, and centrifuging at 1000rpm for 10min to obtain Chinese medicinal extractive solution. Concentrating the Chinese medicinal extractive solution at 80deg.C for 30min, pulverizing the concentrated solid, sieving with 800 mesh sieve, and collecting the undersize to obtain Chinese medicinal extract.
Test examples
The test was performed in city, long-Xingxing county, zhejiang province, city, and city, yao Hongdou village from 11 months 2020 to 5 months 2021. Dwarf 58 wheat was used as the subject.
Test agent: medicament 1: the tebuconazole and the traditional Chinese medicine extract prepared in the example 1 are mixed according to the mass ratio of 2:25, 2g of tebuconazole is calculated per mu, and the mixture of tebuconazole and the traditional Chinese medicine extract is diluted by 500 times by adding water;
medicament 2: tebuconazole and the traditional Chinese medicine extract prepared in the example 2 are mixed according to the mass ratio of 2:25, and the use method is the same as that of the medicament 1;
medicament 3: tebuconazole and the traditional Chinese medicine extract prepared in example 3 are mixed according to a mass ratio of 2:25, and the use method is the same as that of the medicament 1.
8g of tebuconazole is applied per mu, water is added for dilution by 500 times as a control, and water is used as a blank.
Meanwhile, the following comparative agents were set:
comparative agent 1: 30 parts of selfheal are taken and the selfheal extract is prepared according to the method of example 1. Mixing tebuconazole and Prunella vulgaris extract according to a mass ratio of 2:25, and using the same as the medicament 1;
comparative agent 2: 30 parts of lithospermum erythrorhizon was taken and lithospermum erythrorhizon extract was prepared according to the method of example 1. Mixing tebuconazole and radix Arnebiae extract at a mass ratio of 2:25, and using method same as that of the preparation 1;
comparative agent 3: 30 parts of clove are taken and a clove extract is prepared according to the method of example 1. Mixing tebuconazole and flos Caryophylli extract at a mass ratio of 2:25, and using method as in the preparation 1;
comparative agent 4: mixing the prepared selfheal extract, lithospermum extract and clove extract according to the mass ratio of 1:1:1 to obtain a traditional Chinese medicine extract mixture, mixing tebuconazole with the traditional Chinese medicine extract according to the mass ratio of 2:25, and using the same medicament 1.
The tebuconazole used in the medicaments is 80% tebuconazole water dispersible granule, which is purchased from Jiangsu Dongbao agricultural chemical Co., ltd. The tebuconazole is used in an amount calculated by the content of the active ingredients.
Cell setting: 9 cells are arranged according to the grouping, each cell is repeated 3 times, 27 cells are arranged, and the area of each cell is 100m 2 。
Sowing wheat in the period of 5 months in the year 2020, watering once after sowing, and watering in time after the wheat turns green, the period of jointing and the period of blooming after spring starting. Soil preparation is carried out before wheat sowing, and a nitrogen-phosphorus-potassium compound fertilizer (purchased from Gansu Su land fertilizer industry Co., ltd.) is applied as a base fertilizer according to the proportion of 50 kg/mu, and urea is applied in the tillering stage and the jointing stage of the wheat, wherein the urea is 45 kg/mu.
The preparation is sprayed once when the wheat enters the green returning period, the rising period and the jointing period, and is sprayed every time between 5:00 and 6:00 am. And observing and counting the incidence of the sheath blight of wheat on the 10 th and 20 th days after the pesticide is sprayed in the returning period and on the 7 th day after the pesticide is sprayed in the rising period and on the 10 th day after the pesticide is sprayed in the jointing period. The results are shown in Table 1.
TABLE 1 different treatments of the disease states of Rhizoctonia cerealis
Note that: the number of surveys in the table is the average number of surveys per cell; the morbidity is the average morbidity per cell.
As can be seen from Table 1, the incidence of the sheath blight of the blank group on the 10 th day of the wheat reversion period is 5.75%, but the sheath blight of the other medicament groups is not found, which indicates that the compound medicaments of tebuconazole and tebuconazole with the traditional Chinese medicine extract can effectively inhibit the occurrence of the sheath blight of the early stage. By the 20 th day of the wheat green-turning period, the incidence rate of the sheath blight of the blank group is increased, the incidence rate reaches 14.52%, and in addition, the incidence conditions of different degrees appear in the 1-3 groups (2.32%, 2.76% and 4.08%) of the comparison agents. But no banded sclerotial blight was found in other treatments.
By the period of the rise of the wheat, the morbidity of the blank control group reaches 33.8 percent, the disease condition is seriously worsened by the period of the jointing, and the morbidity of the sheath blight reaches 73.76 percent; the contrast agents 1 to 3 have slight sheath blight due to the early stage of reversion, but the control effect of the contrast agents 1 to 3 is weaker, so that the condition of the wheat is aggravated in the period of rising, the condition is aggravated in the period of jointing, and the incidence rate of the sheath blight of the wheat is further up to 27.20%, 23.52% and 58.84%; meanwhile, the contrast agent 4 is found to have a large number of wheat sheath blight plants in the period of the rising of wheat, the morbidity reaches 25.72 percent, and the morbidity reaches 35.64 percent in the period of the jointing, which shows that the contrast agent 4 has remarkable prevention and treatment effects on the early wheat sheath blight, and has insufficient prevention and treatment effects in the later period of the onset of the disease; the other medicament groups have better prevention effect, the incidence rate is not more than 3% in the period of the rising of the wheat, and the disease condition is not further aggravated until the period of the coming-down wheat jointing. The medicaments 1-3 and the control can effectively prevent and control the sheath blight of wheat.
From the above examples, the invention provides a tebuconazole compound bactericidal composition, which is prepared by compounding tebuconazole and traditional Chinese medicine extracts. The bactericidal composition has a strong inhibition effect on the sheath blight of wheat, and the prevention effect on the sheath blight of wheat in the field can reach more than 97 percent, which is equivalent to the prevention effect of high-dose tebuconazole. Therefore, the bactericidal composition provided by the invention can be used for replacing tebuconazole for preventing and controlling banded sclerotial blight in wheat fields.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. The tebuconazole compound bactericidal composition is characterized in that the mass ratio of tebuconazole to traditional Chinese medicine extracts in the bactericidal composition is 1-3: 20-30 parts;
the traditional Chinese medicine extract is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of selfheal, 5-15 parts of lithospermum, and 5-15 parts of clove;
the preparation method of the traditional Chinese medicine extract comprises the following steps:
(1) Drying Prunellae Spica, radix Arnebiae, and flos Caryophylli, and pulverizing to obtain Chinese medicinal powder;
(2) Mixing the traditional Chinese medicine raw material powder with an ethanol solution, and performing ultrasonic extraction to obtain a traditional Chinese medicine extract;
(3) Concentrating the traditional Chinese medicine extract to obtain the traditional Chinese medicine extract.
2. The sterilizing composition according to claim 1, wherein the temperature of the drying is 40 to 60 ℃ and the time of the drying is 1 to 3 hours.
3. The bactericidal composition of claim 2, wherein the crushed particle size is 100 to 200 mesh.
4. The sterilization composition as claimed in claim 3, wherein the mass-to-volume ratio of the traditional Chinese medicine raw material powder to the ethanol solution is 1g: 10-15 ml.
5. The composition of claim 4, wherein the ethanol solution has a volume concentration of 70 to 90%.
6. The sterilizing composition according to claim 5, wherein the power of the ultrasonic extraction is 200 to 350W for 20 to 40min.
7. The sterilizing composition according to any one of claims 1 to 6, wherein the concentration is heat concentration, the temperature of the heat concentration is 60 to 80 ℃, and the time of the heat concentration is 30 to 60 minutes.
8. Use of the tebuconazole compound bactericidal composition in any one of claims 1 to 7 in the preparation of a medicament for controlling rhizoctonia cerealis.
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| CN106070236A (en) * | 2016-07-05 | 2016-11-09 | 南京农业大学 | A kind of containing kind of bacterium azoles and the bactericidal composition of Tebuconazole and application |
| CN106376612A (en) * | 2016-08-31 | 2017-02-08 | 江苏星源生物科技有限公司 | Rice bakanae disease sterilization composition and application thereof |
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| WO2010103065A1 (en) * | 2009-03-11 | 2010-09-16 | Basf Se | Fungicidal compositions and their use |
| CN106070236A (en) * | 2016-07-05 | 2016-11-09 | 南京农业大学 | A kind of containing kind of bacterium azoles and the bactericidal composition of Tebuconazole and application |
| CN106376612A (en) * | 2016-08-31 | 2017-02-08 | 江苏星源生物科技有限公司 | Rice bakanae disease sterilization composition and application thereof |
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