CN114989829B - Composite lotion for repairing DDTs contaminated soil and application thereof - Google Patents
Composite lotion for repairing DDTs contaminated soil and application thereof Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/02—Extraction using liquids, e.g. washing, leaching, flotation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
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- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention belongs to the technical field of soil remediation, and particularly discloses a compound lotion for remediating DDTs contaminated soil and application thereof. The invention utilizes the solubilization of the surfactant Tween 80 and the ethyl acetate which is a green organic solvent to the DDTs with strong hydrophobicity, thereby improving the absorption of the plant to the DDTs and the degradation of the plant in rhizosphere soil. The composite lotion is regularly applied to the soil in a spraying mode, meanwhile, the water content of the soil is strictly controlled to avoid the occurrence of percolation, so that the plant growth is facilitated, the mixing, the transmission and the uniform distribution of active ingredients in the composite lotion are facilitated, the risk of secondary pollution is reduced, and the repairing efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a compound lotion for remediating DDTs contaminated soil and application thereof.
Background
With the development of social economy, environmental awareness of people is continuously enhanced, and demands of people for safe agricultural products are continuously increased. Meanwhile, china is a country with smaller area of cultivated land per unit area, and limited cultivated land is influenced by various pollutants discharged by chemical fertilizers, pesticides and industrial and agricultural production, so that farmland soil is polluted to different degrees, and the quality of soil environment is reduced. Among them, DDT (drop nasal discharge) has serious harm to human health and environment due to its long-term residue, semi-volatility and high toxicity, and organic pollutants, which have been classified as priority control and reduction by numerous international organizations and countries, can enter human bodies through crops produced in farmlands, cause food safety problems, and are harmful to human bodies. With the development of social economy, environmental awareness of people is continuously enhanced, and demands of people for safe agricultural products are continuously increased. The contradiction between the increasing demands of people and the continuous decline of the soil environment quality is continuously outstanding, people and government have paid general attention to, and a safe, reliable, high-quality and low-cost method for repairing DDT (drop nasal discharge) polluted farmland soil is urgently needed.
DDT (Di Ji Zhi) is 2, 2-bis (p-chlorophenyl) -1, 1-trichloroethane with the chemical formula: c (C) 14 H 9 Cl 5 . DDTs are the general terms of DDT isomers and homologs and mainly include p, p ' -DDT, p ' -DDD, p ' -DDE, and the like. DDTs, a typical representative of organochlorine pesticides, have been widely used to control agricultural pests and malaria diseases, and are widely used worldwide. Although it has been disabled for many years, due to its chemistryThe DDT and its degradation products remain in the soil for a long time, with half-lives ranging from several years to tens of years. Contamination of DDTs remains a global problem and has been documented in the stockholm convention, one of 12 persistent organic contaminants that are prohibited or limited from use worldwide. Therefore, the treatment and repair of DDTs contaminated soil has been one of the hot and difficult points of research in the environmental field.
The current treatment technology of DDTs polluted soil mainly comprises physical repair, chemical repair and biological repair. Phytoremediation is a repair technique that utilizes the ecological function of plant degradation, metabolism, transformation, and rhizosphere microorganisms. The phytoremediation has the advantages of low cost, good effect, no secondary pollution, small side effect on human beings and environment, and the like, and can be directly used for in-situ remediation, so that the phytoremediation becomes one of the pollution soil remediation modes with great potential. Plants play a role in the repair process mainly through the following two processes:
A. rhizosphere degradation, namely, the activity of soil microorganisms around the rhizosphere is improved, and the degradation of organic pollutants is promoted;
B. absorption and transport of organic pollutants by plants.
The degradation of DDTs by plants is mainly catalyzed by P450 enzyme system, peroxidase system, dehalogenase system, etc., benzene ring and alkyl are hydroxylated to become activated water-soluble cytotoxin, then conjugated substances are formed with glutathione, glucose, etc., and then transported to vacuoles, etc. by a transporter. The residual concentration of DDTs is an important factor affecting phytoremediation effect, low concentration (< 0.1 mg/kg) is unfavorable for absorption and enrichment of DDTs by plants, and high concentration (> 5 mg/kg) can produce toxic effect which is unfavorable for degradation of plants, both of which can reduce phytoremediation effect.
Although the organic contaminated soil treated by the phytoremediation technology has the characteristics of easy operation, low cost, no secondary pollution and the like, the DDTs with extremely strong hydrophobicity are easily and strongly adsorbed by the soil, and the slow release from soil solid phase to soil solution severely limits the plant availability degree and the phytoremediation speed, so that the increase of the solubility of the DDTs in water improves the microorganism/plant availability of the DDTs, and becomes a key for improving the efficiency of phytoremediation of the DDTs contaminated soil. In recent years, research hotspots mainly focus on the aspects of repairing organic contaminated soil by using a surfactant and a microorganism repairing technology or strengthening phytoremediation efficiency by using a single surfactant or a surface active substance, but related researches on the capability of strengthening phytoremediation DDTs contaminated soil by using a degradable chemical surfactant and other surface active substances are reported.
Therefore, a technical method capable of repairing DDTs polluted soil safely and stably is needed to be searched.
Disclosure of Invention
The invention aims to provide a compound lotion for repairing DDTs polluted soil and application thereof, so as to overcome the defects of the prior art.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a compound lotion for repairing DDTs contaminated soil comprises Tween 80, ethyl acetate and water, wherein the concentration of Tween 80 is 100mg/L-500mg/L, and the concentration of ethyl acetate is 200-1000mg/L.
Further, the compound lotion for repairing DDTs contaminated soil consists of tween 80, ethyl acetate and water, wherein the concentration of the tween 80 is 100mg/L-500mg/L, and the concentration of the ethyl acetate is 200-1000mg/L; preferably, the concentration of Tween 80 is 200mg/L-500mg/L, and the concentration of ethyl acetate is 200-1000mg/L; preferably, the concentration of Tween 80 is 200mg/L-300mg/L, and the concentration of ethyl acetate is 200-600mg/L.
Preferably, the mass ratio of tween 80 to ethyl acetate is 2.5:2.
The application of the composite lotion for repairing DDTs contaminated soil in repairing DDTs contaminated soil comprises the following steps:
s1, carrying out conventional cultivation pretreatment such as ploughing on soil polluted by DDTs, regulating the water content of the soil, and carrying out conventional fertilization;
s2, uniformly sowing germination-accelerating and white-exposed rape seeds in soil, and starting thinning after 2 weeks;
s3, spraying a composite lotion for repairing DDTs polluted soil from the thinning start to the soil, and spraying once every 7-10 d; to the mature period of rape growth.
Further, in step S1, the concentration of DDTs in the contaminated soil is 1mg/kg to 5mg/kg, the DDTs being p, p ' -DDT, p ' -DDD and/or p, p ' -DDE.
Further, in step S1, the contaminated soil is brown soil and/or red soil.
Further, in the step S1, the water content of the soil is maintained at 60% of the maximum water holding capacity in the field, and the soil is sufficient in nutrients, so that the normal growth of plants can be maintained.
Further, in step S1, the fertilizer is applied at a standard of 35mg/kg nitrogen, 25mg/kg phosphorus, and 30mg/kg potassium.
Further, in step S3, the spraying amount is 400-500L per mu each time.
The composite lotion for repairing DDTs polluted soil and the application method thereof have the following advantages:
1) The composite lotion is safe, efficient, low in cost and easy to operate, and can realize in-situ repair;
2) The invention selects a spraying mode to apply synergistic composite lotion to soil, which is beneficial to uniformly distributing active ingredients in the composite lotion in soil particles and improving treatment effect;
3) The water content of the soil selected by the invention is about 60% of the maximum water holding capacity of the soil field, and the water content is favorable for the growth of plants and the mixing and transmission of active ingredients in the synergistic compound lotion, and is closer to the actual field restoration condition;
4) Tween 80 is a typical polyoxyethylene nonionic surfactant and has the characteristics of small influence on environments such as soil charge, pH and the like, easiness in biodegradation, small ecological toxicity to plants and the like, so that the tween 80 has small adsorption loss in soil, the concentration of effectively solubilizing DDTs in a soil-water repair system is high, the apparent solubility of the DDTs is improved, and the absorption of plants to the DDTs and the degradation of the plants in rhizosphere soil are enhanced; ethyl acetate is used as a biodegradable organic solvent, so that the synergistic effect of the composite eluent can be further improved;
5) The repairing process has little influence on the original environment, is applicable to a wide range of soil types, greatly reduces the risk of secondary pollution when being used for repairing the actual environment, is safe and friendly to the ecological environment, and is beneficial to the subsequent soil recycling.
Drawings
FIG. 1 is comparative experimental data of five different treatments of brown soil in example 1.
FIG. 2 is comparative experimental data of five different treatments of red soil in example 2.
FIG. 3 is comparative data of solubilization experiments of Tween 80-ethyl acetate and ethyl acetate on DDTs.
FIG. 4 shows the solubilization experimental data of different concentrations of Tween 80 for DDTs.
FIG. 5 is comparative data of tween 80-ethyl acetate and ethyl acetate elution experiments on DDTs contaminated soil.
Detailed Description
In order to better understand the purpose of the present invention, a method for repairing DDTs contaminated soil by using the composite lotion and application thereof will be described in further detail below with reference to the accompanying drawings.
The invention promotes the phytoremediation of the DDTs polluted soil by the synergistic compound lotion, and utilizes the solubilization of the synergistic compound lotion formed by compounding the surfactant Tween 80 and the green organic solvent ethyl acetate on the DDTs with strong hydrophobicity to improve the absorption of the plant on the DDTs and the degradation of the plant in the rhizosphere soil;
the synergistic composite lotion is regularly applied to the soil in a spraying mode, meanwhile, the water content of the soil is strictly controlled to avoid the occurrence of percolation, so that the synergistic composite lotion is beneficial to plant growth, the mixing, the transmission and the uniform distribution of active ingredients in the synergistic composite lotion are also beneficial, the risk of secondary pollution is reduced, and the repair efficiency is improved.
Firstly, carrying out conventional pretreatment on soil, determining the content of DDTs in the soil, and determining the main components and the usage amount of the synergistic compound lotion according to the content; the polluted soil is sieved by a 60-mesh sieve, and the water content of the polluted soil is regulated; and then uniformly sowing the plump and uniform-sized rape seeds into soil after germination accelerating and white exposing, thinning after 2 weeks of pot cultivation, and starting to periodically apply Tween 80-ethyl acetate composite lotion into the soil in a spraying mode to repair the DDTs polluted soil.
The concentration of DDTs in the soil was analyzed using gas chromatography, and the test was performed with reference to standard GB/T14550-2003.
In the examples, p' -DDT (standard, purity. Gtoreq.98.0%, manufactured by DeltaCo.) was used as a representative of the organochlorine pesticide DDTs, and in the following examples, DDTs were used unless otherwise specified.
Example 1:
setting 5 treatments, repeating each treatment for 3 times, and adopting a random arrangement design for the experiment, wherein 800g of soil is filled in each pot, and experimental data of 5 treatment groups are shown in figure 1 of the specification:
CK: the same pollution level, no plant species and no synergistic compound lotion sample;
t1: the same pollution level, plant species and no synergistic compound lotion sample;
t2: equivalent contamination level, seed plants, single tween 80 lotion sample (250 mg/L with distilled water as solvent);
t3: equivalent contamination level, seed plants, single ethyl acetate wash samples (200 mg/L with distilled water as solvent);
t4: samples of equivalent pollution levels, seed plants, and periodic application of a quantity of synergistic complex lotions, specific experimental procedures are described below.
The soil adopts brown soil from the dengzhui city of Henan province, the pH is 5.85, the organic matter is 24.4g/kg, DDTs is added into the brown soil, the DDTs pollution concentration in the brown soil is 2.48mg/kg by adopting a gas chromatography analysis method, in order to keep the uniformity of a potting test, the polluted soil is sieved by a 60-mesh sieve, and the water content of the soil is regulated to be about 60% of the maximum water holding capacity of the field;
the fertilizer is respectively applied according to the standards of 35mg/kg of nitrogen, 25mg/kg of phosphorus and 30mg/kg of potassium, and the nutrient solution is supplemented once every two weeks, so that the possibility of nutrient stress in the growth process of rhizosphere microorganisms is avoided, and the plants can be ensured to grow in the environment with sufficient mineral nutrients;
uniformly sowing germination-accelerating and white-exposing rape seeds in a flowerpot, and starting thinning after 2 weeks of pot cultivation, wherein the pot cultivation period is 90d;
the tween 80 and ethyl acetate are compounded into a synergistic compound lotion according to the mass ratio of 2.5:2, distilled water is used as a solvent, namely, the concentration of the tween 80 and the concentration of the ethyl acetate are respectively 250mg/L and 200mg/L. After rape grows for 2 weeks, 160ml synergistic compound lotion (seven times, 1120ml in total) is applied to each rape rhizosphere soil by adopting a spraying mode, the water content of the soil is strictly controlled, the infiltration phenomenon is avoided, and the whole repairing process is carried out under the conditions of 25+/-1 ℃ (daytime, light intensity 15000lx, relative humidity 80%) or 20+/-1 ℃ (night, no light and relative humidity 80%). The potting test lasts for 90d, rhizosphere soil samples are taken at 30, 60 and 90d respectively, after treatment, the concentration of DDTs is analyzed by using a gas chromatography, and as a result, the removal rate of the DDTs treated by the synergistic compound lotion of the embodiment T4 after 90d can reach about 60.8%, and the repair efficiency is improved by about 33.3% compared with that of singly using plants for repair.
Example 2:
setting 5 treatments, repeating each treatment for 3 times, and adopting a random arrangement design for the experiment, wherein 800g of soil is filled in each pot, and experimental data of 5 treatment groups are shown in figure 2 of the specification:
CK: the same pollution level, no plant species and no synergistic compound lotion sample;
t1: the same pollution level, plant species and no synergistic compound lotion sample;
t2: equivalent contamination level, seed plants, single tween 80 lotion samples (250 mg/L);
t3: equivalent contamination level, seed plant, single ethyl acetate wash sample (200 mg/L);
t4: samples of equivalent pollution levels, seed plants, and periodic application of a quantity of synergistic complex lotions, specific experimental procedures are described below.
The method comprises the steps of (1) adopting red soil from Changde of Hunan province, wherein the pH is 5.73, the organic matter is 19.4g/kg, adding DDTs into the red soil, analyzing the pollution concentration of the DDTs in the red soil by adopting a gas chromatography method to be 2.47mg/kg, sieving the polluted soil through a 60-mesh sieve in order to keep the uniformity of a potting test, and regulating the water content of the soil to be about 60% of the maximum water holding capacity of the field;
the fertilizer is respectively applied according to the standards of 35mg/kg of nitrogen, 25mg/kg of phosphorus and 30mg/kg of potassium, and the nutrient solution is supplemented once every two weeks, so that the possibility of nutrient stress in the growth process of rhizosphere microorganisms is avoided, and the plants can be ensured to grow in the environment with sufficient mineral nutrients;
uniformly sowing germination-accelerating and white-exposing rape seeds in a flowerpot, and starting thinning after 2 weeks of pot cultivation, wherein the pot cultivation period is 90d;
the tween 80 and ethyl acetate are compounded into a synergistic compound lotion according to the mass ratio of 2.5:2, distilled water is used as a solvent, namely, the concentration of the tween 80 and the concentration of the ethyl acetate are respectively 250mg/L and 200mg/L. After rape grows for 2 weeks, 160ml synergistic compound lotion (seven times, 1120ml in total) is applied to each rape rhizosphere soil by adopting a spraying mode, the water content of the soil is strictly controlled, the infiltration phenomenon is avoided, and the whole repairing process is carried out under the conditions of 25+/-1 ℃ (daytime, light intensity 15000lx, relative humidity 80%) or 20+/-1 ℃ (night, no light and relative humidity 80%). The potting test is continued for 90d, rhizosphere soil samples are taken at 30, 60 and 90d respectively, and after treatment, the concentration of DDTs is analyzed by using a gas chromatography, so that the removal rate of the DDTs treated by the synergistic compound lotion of the T4 in the embodiment is improved by about 23.5% after 90d compared with that of the DDTs treated by singly using phytoremediation.
Solubilization experiment data of different concentrations of Tween 80 on DDTs
The experiment adopts a batch balance oscillation method, and utilizes tween 80 with a series concentration of 0-500 mg/L, and DDTs with a slightly larger saturated dissolution amount are added into a certain amount of tween 80 solution with different concentrations to carry out solubilization experiment on the DDTs.
As shown in figure 4 of the specification, the single Tween 80 has a relatively obvious solubilization effect on DDT, but the apparent solubility of DDTs is not significantly different when the concentration of the single Tween 80 is increased from 100mg/L to 500 mg/L. Tween 80 was used in the present invention at a concentration of 250mg/L as the intermediate value.
Solubilization experiment of DDTs with Tween 80-ethyl acetate
And adding DDTs slightly larger than saturated dissolution amount into a certain amount of ethyl acetate solution with different concentrations by using 0-1000mg/L series concentration ethyl acetate by adopting a batch equilibrium oscillation method, and carrying out solubilization experiments on the DDTs.
Adopting a batch balance oscillation method, compounding ethyl acetate and Tween 80, controlling the concentration of the Tween 80 to be 250mg/L, regulating the concentration of the ethyl acetate to be 0-1000mg/L, obtaining a series of 0-1000mg/L ethyl acetate and 250mg/L Tween 80 compound lotion, respectively adding DDT slightly larger than saturated dissolution into a certain amount of compound solution, and carrying out solubilization experiments on the DDTs.
As shown in figure 3 of the specification, the ethyl acetate alone has a relatively obvious solubilization effect on DDTs, but the apparent solubility of DDTs is not significantly different when the concentration is increased from 200mg/L to 1000mg/L. The apparent solubility of the DDTs in the Tween 80-ethyl acetate composite lotion is obviously improved compared with the apparent solubility of 250mg/L Tween 80 or single ethyl acetate. The synergistic solubilization of the ethyl acetate and the Tween 80 on the DDTs is shown, and the addition of the ethyl acetate improves the solubilization capacity of the Tween 80-ethyl acetate composite lotion on the DDTs.
Eluting experiment of Tween 80-ethyl acetate on DDTs contaminated soil
The experiment adopts a batch equilibrium oscillation method, and uses 0-1000mg/L series concentration ethyl acetate and 250mg/L Tween 80 to carry out elution experiment on a polluted soil (brown soil) sample with the DDTs pollution level of 2.48 mg/kg.
Adopting a batch balance oscillation method, compounding ethyl acetate and Tween 80, controlling the concentration of Tween 80 to be 250mg/L, regulating the concentration of ethyl acetate to be 0-1000mg/L, obtaining a series of 0-1000mg/L ethyl acetate and 250mg/L Tween 80 compound lotion, and carrying out an elution experiment on a contaminated soil (brown soil) sample with the DDTs contamination level of 2.48 mg/kg.
The elution experiment steps are specifically as follows: 15g of contaminated soil (brown soil) is selected, the compound lotion (15 mL) prepared in advance before the experiment is added into a 50mL glass conical flask, and the elution experiment is carried out by reciprocating oscillation in a constant-temperature water bath oscillator, so that the contaminated soil is fully contacted and mixed with the compound lotion, and after oscillation for 60min, the mixture is kept stand for sedimentation for 12h, so that DDTs in the soil are stripped from the soil. After washing and sedimentation are completed, the material is transferred into a 100mL centrifuge tube, and is subjected to centrifugation at a rotation speed of 5000r/min for 10min, then the material is kept stand to remove the liquid phase solution, and then the solid phase soil at the bottom of the centrifuge tube is taken out and put into a drying box, and is subjected to drying treatment at 105 ℃. The DDTs content in the eluted soil was analyzed by GB/T14550-2003 execution method.
As shown in figure 5 of the specification, the single ethyl acetate has a certain eluting effect on the DDTs polluted soil sample, but the eluting rate of the DDTs does not change significantly as the concentration of the ethyl acetate is increased from 200mg/L to 1000mg/L. The elution rate of the DDTs under the action of the Tween 80-ethyl acetate composite lotion is obviously improved compared with that of the DDTs under the action of 250mg/L Tween 80 or single ethyl acetate. The addition of ethyl acetate improves the eluting capability of the Tween 80-ethyl acetate composite lotion on the soil polluted by the DDTs.
The method has the advantages of economy, high efficiency, easy operation, wide applicable soil types, realization of in-situ restoration, small influence on the original environment in the restoration process, great reduction of secondary pollution risk when being used for the restoration of the actual environment, and contribution to the subsequent soil recycling.
Claims (2)
1. The application of the composite lotion in repairing DDTs contaminated soil is characterized by comprising the following steps:
s1, carrying out conventional cultivation pretreatment on soil polluted by DDTs, wherein the concentration of the DDTs in the polluted soil is 1mg/kg-5mg/kg, regulating the water content of the soil to be 60% of the maximum water holding capacity of the soil, and carrying out conventional fertilization;
s2, uniformly sowing germination-accelerating and white-exposed rape seeds in soil, and starting thinning after 2 weeks;
s3, spraying a composite lotion for repairing DDTs polluted soil from the thinning start to the soil, and spraying once every 7-10 d; until the rape grows to maturity;
the compound lotion in the step S3 consists of tween 80, ethyl acetate and water, wherein the mass ratio of the tween 80 to the ethyl acetate in the compound lotion is 2.5:2, the concentration of the tween 80 is 200mg/L-300mg/L, and the concentration of the ethyl acetate is 200mg/L-600mg/L.
2. Use according to claim 1, characterized in that in step S1 the contaminated soil is brown and/or red.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1322862C (en) * | 1989-03-31 | 1993-10-12 | Tibor Cseh | Process for removal of hydrophobic organic contaminants from solid state materials |
| CN113020248A (en) * | 2021-05-19 | 2021-06-25 | 吉林大学 | Method for repairing DDTs (dichloro-diphenyl-trichloroethane) contaminated soil by using composite lotion and application of composite lotion |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1322862C (en) * | 1989-03-31 | 1993-10-12 | Tibor Cseh | Process for removal of hydrophobic organic contaminants from solid state materials |
| CN113020248A (en) * | 2021-05-19 | 2021-06-25 | 吉林大学 | Method for repairing DDTs (dichloro-diphenyl-trichloroethane) contaminated soil by using composite lotion and application of composite lotion |
Non-Patent Citations (4)
| Title |
|---|
| Tween80-乳酸乙酯复合溶液强化植物吸收土壤中的DDT;郭平 等;东北师范大学报;第49卷(第3期);第151-152页材料与方法部分 * |
| 持久性有机污染场地土壤淋洗法修复研究进展;叶茂;杨兴伦;魏海江;卞永荣;王芳;谷成刚;蒋新;;土壤学报(第04期);摘要 * |
| 潘淑颖等.特异性作物对土壤中DDT降解的诱导作用研究.农业灾害研究.2012,(第6期),摘要、第1.1.5节. * |
| 郭平 等.Tween80-乳酸乙酯复合溶液强化植物吸收土壤中的DDT.东北师范大学报.2017,第49卷(第3期),第151-152页材料与方法部分. * |
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