CN114101321B - Method for absorbing and degrading tri (1-chloro-2-propyl) phosphate in soil by using vetch of leguminous plant - Google Patents
Method for absorbing and degrading tri (1-chloro-2-propyl) phosphate in soil by using vetch of leguminous plant Download PDFInfo
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Abstract
The invention relates to the technical field of organic phosphate flame retardant/plasticizer contaminated soil plant remediation, in particular to a method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by using vetch which is a leguminous plant. The method is characterized in that vetch which is a leguminous plant is planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the tris (1-chloro-2-propyl) phosphate content in the soil is removed or remarkably reduced by utilizing the combined action of enzymes and small molecular substances which are absorbed by the plant and secreted by the root system and a rhizosphere microbial system in the growth process of the plant. The invention adopts the green manure plant vetch which has stronger nitrogen fixation capacity and can provide a large amount of nitrogen fertilizer and organic matters for soil to absorb and degrade the tris (1-chloro-2-propyl) phosphate in the soil, has the advantages of simple operation, low cost, in-situ remediation and the like, can prevent the tris (1-chloro-2-propyl) phosphate in the soil from entering a food chain, and can provide a large amount of organic green manure for soil improvement.
Description
Technical Field
The invention relates to the technical field of phytoremediation of organic phosphate flame retardant/plasticizer contaminated soil, in particular to a method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by using vetch which is a leguminous plant.
Background
Organic Phosphates (OPEs) are widely applied to products such as plastics, electronic products and the like as flame retardants and plasticizers with good flame retardant and plasticizing effects, and the global usage amount of the Organic Phosphates (OPEs) is 68 ten thousand tons in 2015. China is also a big country for producing and using OPEs, and the usage amount of the OPEs in China in 2012 reaches 17.9 ten thousand tons, and the OPEs continue to increase at an average annual growth rate of 15%. The chloroalkyl OPEs are the most important OPEs, are used in an amount of 55 percent of the total use amount of the OPEs, and mainly comprise three of tris (2-chloroethyl) phosphate (TCEP), tris (1-chloro-2-propyl) phosphate (TCIPP) and tris (1, 3-dichloroisopropyl) phosphate (TDCPP). However, TCEP has limited production and use due to its carcinogenic, neurotoxic, and adverse reproductive effects, among others. TCIPP is an alternative to TCEP, and its production and use are rapidly increasing after TCEP is limited. However, TCIPP has been shown to reduce cell numbers, alter neural differentiation, stimulate the skin and eyes of rats, accumulate in the liver and kidney, and is a potential carcinogen. More importantly, TCIPP is added to the final product primarily in an additive rather than chemical bonding manner, which makes it very easy to release into the surrounding environment. Existing monitoring data indicate that TCIPP is the most prominent OPEs in environmental media such as water, soil, atmosphere and sediments. For example, the detection rate of TCIPP in urban surface water of Beijing in China is as high as 99.4%, the average concentration is 291ng/L, and the detection rate accounts for 30.5% of the total concentration of OPEs; the concentration of the compound in farmland soil in China is as high as 401 mug/kg, and accounts for 39.6 percent of the total concentration of OPEs. Therefore, there is a need to develop the pollution control and repair technology research of OPEs based on TCIPP.
At present, the abatement technology for OPEs mainly focuses on the abatement of OPEs by chemical oxidation or microbial degradation. For example, luguining et al degrade TCEP using pyrite activated persulfate, lin hai et al degrade TCEP and tributyl phosphate (TBP) using thermocatalytic potassium persulfate and UV radiation hydrogen peroxide oxidation; yi Hua et al screened a brevibacillus brevis capable of efficiently degrading triphenyl phosphate (TPP) from the soil in the electronic waste dismantling area, and Linhai et al screened a sphingomonas capable of degrading TBP from the tailings sand. However, these studies do not relate to TCIPP, and TCIPP structure and properties have great differences from TCEP, TBP, etc., and the above-mentioned methods cannot be easily applied to TCIPP. Furthermore, these studies are currently carried out in pure systems, and how to reduce the effects of OPEs in practical environments, particularly in soil, remains to be investigated. Moreover, oxidative degradation technologies such as thermal catalysis and UV radiation can only be used for ex-situ remediation of contaminated soil, and the practicability of farmland soil with high TCIPP content and large quantity and wide range is poor. Therefore, it is necessary to develop the bioremediation of TCIPP contaminated soil, especially the research of phytoremediation technology, to ensure the safe production of TCIPP contaminated farmland soil.
The mechanism of plant repairing organic pollutant mainly includes 3 kinds, including direct absorption of organic matter by root system, releasing secretion by root system, decomposing organic matter with enzyme, strengthening degradation of organic matter by rhizosphere microflora, etc. At present, plant repairing research on traditional organic pollutants such as polycyclic aromatic hydrocarbon and polychlorinated biphenyl is reported more, but plant repairing of a novel pollutant TCIPP in soil is rarely reported, and the TCIPP is also a novel organic pollutant with properties and structure which are greatly different from those of the traditional organic pollutants such as polycyclic aromatic hydrocarbon and polychlorinated biphenyl. Therefore, screening plants which can effectively absorb and degrade TCIPP in soil is very important. In addition, in order to avoid contaminants in the soil entering the food chain during remediation, it is a better choice to select non-edible plants, including green manure plants, as the remediation plants. The green manure plants not only can repair soil pollution, but also can provide a large amount of organic green manure for soil improvement.
Disclosure of Invention
The invention aims to provide a method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by using vetch (Vicia villosa L.) which can be used as a green manure plant.
In order to realize the purpose, the invention adopts the technical scheme that:
a method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by using vetch which is a leguminous plant is characterized in that: the method is characterized in that vetch which is a leguminous plant is planted in the soil polluted by the tri (1-chloro-2-propyl) phosphate, and the content of the tri (1-chloro-2-propyl) phosphate in the soil is removed or remarkably reduced by utilizing the combined action of enzymes and small molecular substances secreted by a root system and a microorganism system in a rhizosphere ring in the growth process of the plant.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant is characterized by comprising the following steps of: the concentration of the phosphoric acid tri (1-chloro-2-propyl) ester in the polluted soil is 0.5-1mg/kg.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant is characterized by comprising the following steps of: before sowing vetch, applying base fertilizer into the soil, wherein the base fertilizer is calcium superphosphate, and the application amount is 0.05-0.1g of calcium superphosphate/kg of soil.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant is characterized by comprising the following steps of: the seed coat of the vetch is cut by scissors and then soaked in warm water at 25-35 ℃ for imbibition for 8-12h.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant is characterized by comprising the following steps of: the vetch seeds after germination acceleration are directly sown in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and then the soil is covered by 2-3cm.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant is characterized by comprising the following steps of: watering is carried out irregularly, so that the water content of the soil is always kept between 60 and 80 percent of the field water capacity (the field water capacity refers to the highest soil water content which can be stably kept by the soil and is expressed by percentage of the soil volume).
The vetch is planted or continuously planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the tris (1-chloro-2-propyl) phosphate in the soil is absorbed and degraded by the absorption of the vetch, enzymes and small molecular substances secreted by a root system and the combined action of a microbial system in a rhizosphere ring until the content of the tris (1-chloro-2-propyl) phosphate in the soil reaches the environmental safety standard.
Vetch (Vicia villosa L.) adopted by the invention is an annual herbaceous plant belonging to the genus Vicia in the family Leguminosae. The general inflorescence, the pod is short and rectangular, the width is 0.6-1cm, the length is 2-3cm, each pod has about 3 seeds, the seeds are oval or round, and most of the seeds are black brown; the pedicel grows from the axilla of the leaf, one inflorescence is that 10-30 flowers bloom on each peduncle, and the number of the single plant inflorescence can reach about two hundred. The vetch is not only a good feed for livestock and poultry, but also an important green manure crop in paddy fields and cotton fields. The vetch has strong nitrogen fixation capacity, can provide a large amount of nitrogen fertilizer and organic matters for soil after being crushed and applied to the soil, and is an ideal green manure plant.
The invention has the following advantages:
1. according to the invention, vetch is planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the tris (1-chloro-2-propyl) phosphate in the soil is absorbed and degraded by using the vetch which can be used as a green manure plant. The method has the advantages of simple operation, low cost, in-situ repair and the like; moreover, as a green manure plant which has strong nitrogen fixation capacity and can provide a large amount of nitrogen fertilizer and organic matters for soil, the planted vetch can not only effectively reduce the content of the tris (1-chloro-2-propyl) phosphate in the soil, but also can prevent the tris (1-chloro-2-propyl) phosphate in the soil from entering a food chain, and simultaneously can also provide a large amount of organic green manure for soil improvement;
2. according to the invention, the vetch is planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and when the concentration of the tris (1-chloro-2-propyl) phosphate in the soil is 0.5-1mg/kg, the growth amount of the vetch is not inhibited basically. After planting for 45 days, the gathering coefficient of the vetch to the tris (1-chloro-2-propyl) phosphate in the soil is 1.22, and the transfer coefficient from the underground part (root) to the overground part (stem and leaf) is 2.56. The results show that the vetch can effectively absorb the tris (1-chloro-2-propyl) phosphate in the soil and can better transport the tris (1-chloro-2-propyl) phosphate from the underground part to the overground part, and the vetch is an enrichment plant of the tris (1-chloro-2-propyl) phosphate;
3. according to the invention, vetch is planted in the tris (1-chloro-2-propyl) phosphate contaminated soil, and when the concentration of the tris (1-chloro-2-propyl) phosphate in the soil is 0.5-1mg/kg and after the planting is carried out for 45 days, the vetch effectively reduces the tris (1-chloro-2-propyl) phosphate in the contaminated soil by utilizing the combined action of the enzyme and the small molecular substances secreted by the self absorption and the root system of the vetch and the microbial system of the rhizosphere ring. The rate of decrease of tris (1-chloro-2-propyl) phosphate in vetch rhizosphere soil is 86.1%, the rate of decrease of tris (1-chloro-2-propyl) phosphate in non-rhizosphere soil is 65.5%, and the rate of decrease of tris (1-chloro-2-propyl) phosphate in non-plant control soil is only 10.5%. The result shows that the vetch has stronger tolerance to the pollution of the tris (1-chloro-2-propyl) phosphate, and can effectively reduce the tris (1-chloro-2-propyl) phosphate in the polluted soil through absorption, degradation and other modes;
4. the invention utilizes the green manure plant vetch which has stronger nitrogen fixation capacity and can provide a large amount of nitrogen fertilizer and organic matters for soil to absorb and degrade the tris (1-chloro-2-propyl) phosphate in the soil, can not only obviously reduce the concentration of the tris (1-chloro-2-propyl) phosphate in the soil, but also can be used as green manure to be applied to the soil after being crushed to provide a large amount of nitrogen fertilizer and organic matters for the soil, and reduces the problem of secondary pollution possibly caused in the restoration process while improving the environmental quality of the soil. Therefore, it is feasible to screen plants capable of absorbing and degrading the tris (1-chloro-2-propyl) phosphate from the green manure plants and popularize and apply the plants.
In a word, the vetch is planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the purpose of effectively absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil can be achieved by utilizing the combined action of the enzyme and the small molecular substance secreted by the plant and the microbial system of the rhizosphere ring. By continuously planting the plant and repeating the steps, the tri (1-chloro-2-propyl) phosphate in the polluted soil can be continuously reduced until the content of the tri (1-chloro-2-propyl) phosphate reaches the environmental safety standard. The method has the advantages of simple operation, low cost, capability of being used for in-situ remediation, capability of providing a large amount of organic green manure for soil improvement while remediation and the like.
Drawings
FIG. 1 is a biomass map of roots, stems and leaves of vetch planted in tris (1-chloro-2-propyl) phosphate contaminated soil and blank control soil after 45 days provided by the embodiment of the invention;
FIG. 2 is a graph showing the content of tris (1-chloro-2-propyl) phosphate in roots, stems and leaves after planting vetches in tris (1-chloro-2-propyl) phosphate contaminated soil and blank control soil for 45d, which is provided by the embodiment of the invention;
FIG. 3 is a graph showing the reduction rate of tris (1-chloro-2-propyl) phosphate in rhizosphere and non-rhizosphere soil and non-plant control soil after the vetch is planted in the tris (1-chloro-2-propyl) phosphate contaminated soil 45d according to the embodiment of the invention.
Detailed Description
A method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by utilizing vetch (Vicia villosa L.) of a leguminous plant mainly comprises the following steps:
the method is characterized in that vetch which is a leguminous plant is planted in the soil polluted by the tri (1-chloro-2-propyl) phosphate, and the content of the tri (1-chloro-2-propyl) phosphate in the soil is removed or remarkably reduced by utilizing the combined action of enzymes and small molecular substances secreted by a root system and a microorganism system in a rhizosphere ring in the growth process of the plant.
The method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant needs to apply the base fertilizer in the soil before sowing, wherein the base fertilizer is the calcium superphosphate, and the application amount is 0.05-0.1g of the calcium superphosphate per kg of the soil.
According to the method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by using the vetch of the leguminous plant, the seed coat of the vetch needs to be cut by scissors before sowing, and then the vetch is soaked in warm water at the temperature of 25-35 ℃ and is swelled for 8-12 hours.
The planting method is that vetch seeds after germination accelerating are directly sown in the tris (1-chloro-2-propyl) phosphate polluted soil, and then 2-3cm of soil is covered.
According to the method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant, watering is required to be carried out at irregular intervals in the growth process of the vetch, so that the water content of the soil is always kept at 60-80% of the field water capacity (the field water capacity refers to the highest soil water content which can be stably kept by the soil and is expressed by percentage of the soil volume).
According to the method for absorbing and degrading the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch which is a leguminous plant, the vetch is planted or continuously planted in the tris (1-chloro-2-propyl) phosphate polluted soil, and the vetch absorbs and degrades the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the combined action of the enzyme and the small molecular substance secreted by the absorption and the root system of the vetch and the microbial system of the rhizosphere until the content of the tris (1-chloro-2-propyl) phosphate in the soil reaches the environmental safety standard.
Examples
The experimental site is arranged in a greenhouse of Shenyang university, and the soil for potted plant experiments adopts blank control soil and tris (1-chloro-2-propyl) phosphate contaminated soil. The blank control soil is collected from soil of a certain farmland in a new Shenbei district in Shenyang city, and has the following basic physicochemical properties: the pH value is 7, the organic matter content of the soil is 14.7g/kg, the total nitrogen content is 0.65g/kg, the contents of alkaline hydrolysis nitrogen, available phosphorus and quick-acting potassium are 46.8, 33.6 and 69.3mg/kg respectively, and the background value of the tris (1-chloro-2-propyl) phosphate in the soil is 0.029mg/kg; the tris (1-chloro-2-propyl) phosphate contaminated soil is prepared from blank control soil by adding a tris (1-chloro-2-propyl) phosphate standard substance, and the prepared contaminated soil is kept stand and aged for 6 months, wherein the content of the tris (1-chloro-2-propyl) phosphate is 0.84mg/kg. The vetch seed planting method comprises the steps of shearing seed coats of vetch seeds by using scissors, soaking and swelling for 8-12 hours in warm water at the temperature of 25-35 ℃, then directly sowing the seed after germination acceleration in experimental soil in which base fertilizer is applied in advance, wherein the application amount of the base fertilizer is 0.05-0.1g of calcium superphosphate/kg of soil, and then covering soil for 2-3cm. The experiment was carried out while setting a blank control group (i.e., vetch was planted in the blank control soil) and a no-plant control group (i.e., tris (1-chloro-2-propyl) phosphate contaminated soil without plants). The experimental pot is a circular PE plastic pot with the height of 12cm and the diameter of 15cm, the mass of soil in each pot is 0.7kg, 20 seeds are uniformly sown in each pot, thinning is 10 seeds/pot after the growth is stable, watering is carried out by pure water in the growth period, and each treatment level is repeated for 6 times. The irrigation frequency and the irrigation water amount are controlled in the experimental process to prevent the tri (1-chloro-2-propyl) phosphate in the soil from losing. Meanwhile, 3 pots of the plant-free control group are arranged, and the same irrigation operation is applied at ordinary times. After the plants are planted for 45 days, roots, stems and leaves of the plants are harvested respectively, rhizosphere soil and non-rhizosphere soil and plant-free control soil are collected, and the content of the tris (1-chloro-2-propyl) phosphate in the plants and the soil is measured.
The content of the tris (1-chloro-2-propyl) phosphate in the soil is determined by ultrasonic extraction and gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS). Accurately weighing 5g of freeze-dried soil sample, placing the soil sample in a polytetrafluoroethylene centrifugal tube, and adding 10ng of internal standard substance deuterated tri-n-butyl phosphate (TnBP-d) 27 ) Stirring uniformly; then 20mL of a 1:1, carrying out vortex for 3min, carrying out ultrasonic for 20min, and centrifuging for 10min at 5000r/min, and collecting supernate; repeating the above process once, transferring the supernatant into a heart-shaped bottle, rotary evaporating to near dryness, and metering volume with chromatographically pure hexaneTo 1mL, as determined by GC-MS/MS analysis.
The content of the phosphoric acid tri (1-chloro-2-propyl) ester in the plant is determined by matrix solid phase dispersion extraction and gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS). Accurately weighing 0.5g of plant sample crushed by a high-speed tissue triturator, and putting the plant sample into a glass mortar; adding 20ng of internal standard substance deuterated tri-n-butyl phosphate (TnBP-d) 27 ) 2g of Florisil, 2g of anhydrous sodium sulfate and 0.1g of graphitized carbon black, and uniformly grinding; transferring the uniformly ground mixture to an empty solid phase extraction column which is placed in a sieve plate, covering the sieve plate and compacting by using a syringe piston; using 15mL of the mixture with the volume ratio of 1:1, washing a mortar and a pestle by using the n-hexane-acetone mixed solvent for three times, and transferring the mixed solvent into a solid phase extraction column to elute a target compound; collecting eluate, concentrating at room temperature under nitrogen blowing to near dryness, adding chromatographically pure hexane to a constant volume of 1mL, and determining by GC-MS/MS analysis.
FIG. 1 shows the biomass of the roots, stems and leaves of vetch 45d planted in the soil polluted by tris (1-chloro-2-propyl) phosphate and the blank control soil. Under the stress of 0.84mg/kg of tris (1-chloro-2-propyl) phosphate pollution, the total biomass of roots, stems, leaves and single plants of the vetch is reduced from 0.22 g, 0.48 g, 0.24 g and 0.94g to 0.21 g, 0.46 g, 0.22 g and 0.90g respectively after the vetch is planted for 45 days compared with a control. The results show that the tris (1-chloro-2-propyl) phosphate has a certain inhibiting effect on the growth of sweet potato, but the inhibiting effect is not obvious, and the growth of the sweet potato is not influenced basically.
FIG. 2 shows the content of tris (1-chloro-2-propyl) phosphate in roots, stems and leaves of vetch 45d planted in tris (1-chloro-2-propyl) phosphate contaminated soil and blank control soil. Under the stress of 0.84mg/kg of tris (1-chloro-2-propyl) phosphate pollution, after the vetch is planted for 45 days, the concentrations of the tris (1-chloro-2-propyl) phosphate in roots, stems and leaves of the vetch are 458, 294 and 3004 mug/kg respectively, which are obviously higher than the concentrations of the tris (1-chloro-2-propyl) phosphate in the roots, stems and leaves of the vetch planted in the blank control soil. The results show that the vetch can absorb the tris (1-chloro-2-propyl) phosphate in the soil through the root system, and the tris (1-chloro-2-propyl) phosphate is transported to the leaves through the stem and finally accumulated in the leaves. The enrichment coefficient of vetch to the tris (1-chloro-2-propyl) phosphate in the soil is 1.22, and the transport coefficient of the vetch from the underground part (root) to the overground part (stem and leaf) is 2.56, which indicates that the vetch is a tris (1-chloro-2-propyl) phosphate enrichment plant. .
FIG. 3 shows the reduction rate of tris (1-chloro-2-propyl) phosphate in rhizosphere and non-rhizosphere soil of vetch 45d planted in tris (1-chloro-2-propyl) phosphate contaminated soil and non-plant control soil. Under the stress of 0.84mg/kg of tris (1-chloro-2-propyl) phosphate pollution, after the vetch is planted for 45 days, the rate of decrease of tris (1-chloro-2-propyl) phosphate in vetch rhizosphere soil is 86.1%, the rate of decrease of tris (1-chloro-2-propyl) phosphate in non-rhizosphere soil is 65.5%, and the rate of decrease of tris (1-chloro-2-propyl) phosphate in plant-free control soil is only 10.5%. Wherein, the contribution rate of the vetch to the reduction of the tris (1-chloro-2-propyl) phosphate in the soil through self extraction and absorption is 1.52 percent, and the vetch has higher extraction and repair efficiency (generally lower than 1 percent). The content of the tris (1-chloro-2-propyl) phosphate in the polluted soil is effectively reduced by the absorption of the vetch and the synergistic effect of the root system and rhizosphere microorganisms.
Experiments show that the vetch has stronger tolerance and higher absorption and degradation capability to the pollution of the tris (1-chloro-2-propyl) phosphate, the vetch is planted or continuously planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the absorption, the enzyme secreted by the root system, the small molecular substance and the combined action of a microbial system of a rhizosphere of the vetch absorbs and degrades the tris (1-chloro-2-propyl) phosphate in the soil until the content of the tris (1-chloro-2-propyl) phosphate in the soil reaches the environmental safety standard.
Claims (4)
1. A method for absorbing and degrading tris (1-chloro-2-propyl) phosphate in soil by utilizing vetch which is a leguminous plant is characterized by comprising the following steps: the method is characterized in that the legume plant vetch is planted in the soil polluted by the tris (1-chloro-2-propyl) phosphate, and the plant removes or obviously reduces the content of the tris (1-chloro-2-propyl) phosphate in the soil by utilizing the combined action of the absorption of the plant, the enzyme and the small molecular substance secreted by the root system and the microbial system of the rhizosphere ring in the growth process;
when the vetch is planted, watering is carried out irregularly, so that the water content of the soil is always kept to be 60-80% of the field water capacity;
the concentration of the phosphoric acid tri (1-chloro-2-propyl) ester in the polluted soil is 0.5-1mg/kg.
2. The method for absorbing and degrading the tri (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant, according to claim 1, is characterized in that: before sowing vetch, applying base fertilizer into the soil, wherein the base fertilizer is calcium superphosphate, and the application amount is 0.05-0.1g of calcium superphosphate/kg of soil.
3. The method for absorbing and degrading the tri (1-chloro-2-propyl) phosphate in the soil by utilizing the sweet potatoes of the leguminous plant according to claim 1, wherein the method comprises the following steps: the seed coat of the sweet potato seed is cut by scissors and then soaked in warm water at the temperature of 25-35 ℃ for 8-12h for imbibition.
4. The method for absorbing and degrading the tri (1-chloro-2-propyl) phosphate in the soil by utilizing the vetch of the leguminous plant, according to claim 1, is characterized in that: the vetch seeds after germination acceleration are directly sown in the tris (1-chloro-2-propyl) phosphate polluted soil, and then the soil is covered by 2-3cm.
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| CN110883084B (en) * | 2019-12-02 | 2021-08-17 | 淮阴工学院 | Method for in-situ remediation of soil phthalate pollution by using mushroom dregs and alfalfa |
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