CN114989285B - Multifunctional mulberry silk sericin and application thereof in biomedical materials - Google Patents
Multifunctional mulberry silk sericin and application thereof in biomedical materials Download PDFInfo
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- CN114989285B CN114989285B CN202210682578.4A CN202210682578A CN114989285B CN 114989285 B CN114989285 B CN 114989285B CN 202210682578 A CN202210682578 A CN 202210682578A CN 114989285 B CN114989285 B CN 114989285B
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- mulberry silk
- quercetin
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- OVSQVDMCBVZWGM-DTGCRPNFSA-N quercetin 3-O-beta-D-galactopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-DTGCRPNFSA-N 0.000 description 1
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- 235000005493 rutin Nutrition 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0045—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C3/00—Treatment of animal material, e.g. chemical scouring of wool
- D01C3/02—De-gumming silk
Abstract
The invention provides a multifunctional mulberry silk fibroin and application thereof in biomedical materials. The multifunctional mulberry silk sericin is prepared by the following method: feeding mulberry leaves with quercetin suspension applied to the surface of the mulberry leaves to silkworms, and removing silkworm chrysalis in silkworm cocoons obtained by spinning the silkworms to obtain cocoon shells; separating sericin from cocoon shell to obtain the multifunctional mulberry silk sericin. The multifunctional mulberry silk fibroin has better oxidation resistance and ultraviolet absorption performance, can emit bright yellow-green light under the excitation of 365nm laser, and has high-intensity fluorescence performance.
Description
Technical Field
The invention belongs to the technical field of biomedicine. More particularly, to a multifunctional mulberry silk fibroin and application thereof in biomedical materials.
Background
The silkworm cocoon mainly comprises sericin and silk fibroin, wherein the sericin is wrapped on the surface layer of silk fibroin fiber. Sericin is a natural macromolecular protein, has good biocompatibility and a series of unique biological performances, is a natural biological material with excellent performances, and is widely applied to the fields of fabric after-finishing coatings, cosmetic additives, food, medical functional biological materials and the like. Although the comprehensive performance evaluation of sericin is better, the sericin obtained by directly separating and extracting from silkworm cocoons has the defect of low oxidation resistance and ultraviolet absorption, which limits the application of the mulberry silk fibroin to a certain extent and limits the further development of silk industry. In order to give higher added value to the mulberry silk fibroin, a great deal of scientific researchers are devoted to the study of the functional modification of the mulberry silk fibroin, such as the mulberry silk fibroin with oxidation resistance, the mulberry silk fibroin with excellent ultraviolet absorption performance, the mulberry silk fibroin with fluorescence performance, and the like.
At present, the feeding method shows great advantages in the functional modification method of the mulberry silk fibroin, the method is generally used for feeding mulberry silkworms after the additive is added into mulberry leaves, is simple, efficient, energy-saving and environment-friendly, has high biological safety, is beneficial to large-scale production, can improve the performance of silk at the source, but the traditional additive is mainly quantum dots, metal nanoclusters, organic fluorescent dyes and the like, and basically needs to be prepared by complex and harsh processes, so that the environment is possibly influenced, the normal growth of the mulberry silkworms is possibly influenced, and the defects seriously influence the development and application of the feeding method in the functional modification of the mulberry silk fibroin, so that the additive which is friendly to the environment and simple in preparation process is searched, and has quite necessity for the development and application of the feeding method in the functional modification of the mulberry silk fibroin.
Quercetin (Quercetin) is flavonol compound with various biological activities, and has chemical formula of C 15 H 10 O 7 Is widely present in the bark, flowers, leaves, buds of many plantsIn seeds and fruits, the quercetin is obtained by acid hydrolysis of rutin, quercetin, hyperin, etc. Wherein the content of semen Fagopyri Esculenti in the stem and leaf, fructus Hippophae, fructus crataegi, and Bulbus Allii Cepae is high. Quercetin is also found in many foods such as onion, chives, asparagus, cabbage, mustard, green pepper, chicory, grapefruit, lettuce, hawthorn, apple, mango, prune, radish, blackcurrant, potato, spinach, and the like. In addition, about 100 medicinal plants (such as flos Sophorae Immaturus, folium Platycladi, rhizoma Alpiniae Officinarum, flos Farfarae, herba Taxilli, notoginseng radix, semen Ginkgo, and ramulus Sambuci Williamsii) contain the above components. Based on the advantages of wide sources, simple extraction process, environmental friendliness and the like, the application of the quercetin gradually becomes a research hotspot at present, such as: the application of quercetin in plant senescence promoters, as follows: application of quercetin in preparing medicament for treating mitochondrial dysfunction related diseases is provided. However, no related research report on the aspect of using quercetin as an additive for silkworm feeding is yet seen.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a multifunctional mulberry silk fibroin and application thereof in biomedical materials, and the functional modification of the mulberry silk fibroin is realized.
The first object of the invention is to provide a multifunctional mulberry silk fibroin.
The second object of the invention is to provide the application of the multifunctional mulberry silk fibroin as a daily chemical material and/or biomedical material and/or food.
The above object of the present invention is achieved by the following technical scheme:
the invention provides a multifunctional mulberry silk fibroin, which is prepared by the following steps: feeding mulberry leaves with quercetin suspension applied to the surface of the mulberry leaves to silkworms, and removing silkworm chrysalis in silkworm cocoons obtained by spinning the silkworms to obtain cocoon shells; separating sericin from cocoon shell to obtain the multifunctional mulberry silk sericin.
According to the invention, after a series of treatments are carried out on silkworm cocoons obtained by feeding quercetin to silkworms, the obtained multifunctional mulberry silk fibroin has good biocompatibility and biodegradability of the mulberry silk fibroin, has good oxidation resistance and ultraviolet absorption performance, can emit bright yellow-green light under the excitation of 365nm laser, has high-intensity fluorescence performance, can be clinically used for marking and tracking, and can be prepared into biomedical materials with good performance, such as biological dressing, drug slow-release carrier materials, in-vivo imaging materials, detection and analysis materials and the like.
Preferably, the applying comprises coating, spraying, or the like.
Preferably, the feeding stage controls the dosage of quercetin unchanged, and ensures that silkworm can eat freely.
Preferably, the silkworms are silkworms of the second day of five ages to the seventh day of five ages.
Most preferably, the silkworm is a five-year old silkworm on the third day.
Preferably, the mass ratio of quercetin to mulberry leaf is 1-5: 100.
most preferably, the mass ratio of quercetin to mulberry leaf is 2.5:100.
preferably, the concentration of quercetin suspension is 0.3-0.5 g/mL.
Most preferably, the concentration of quercetin suspension is 0.33g/mL.
Preferably, the separation is to soak cocoon shells in water and then heat the cocoon shells for 1 to 4 hours at a temperature of between 60 and 100 ℃.
Most preferably, the separation is by immersing the cocoon shells in distilled water and heating at 90 ℃ for 1 hour.
Preferably, the mass-volume ratio of the cocoon shell to the water is 1g: 10-100 mL.
Most preferably, the mass-to-volume ratio of the cocoon shell to the water is 1g:50mL.
Preferably, the separation is followed by filtration and lyophilization.
Further preferably, the filtration is vacuum filtration.
Further preferably, the filtration is performed by using 400-500 mesh filter cloth.
Further preferably, the freeze-drying is performed at-70 to-50 ℃ for 40 to 60 hours.
Most preferably, the lyophilization is at-55 ℃ for 50 hours.
According to the invention, after a series of treatments are carried out on silkworm cocoons obtained by feeding quercetin to silkworms, the obtained multifunctional mulberry silk fibroin has good biocompatibility and biodegradability of the mulberry silk fibroin, has good oxidation resistance and ultraviolet absorption performance, can emit bright yellow-green light under the excitation of 365nm laser, has high-intensity fluorescence performance, can be clinically used for marking and tracking, and can be prepared into biomedical materials with good performance, such as biological dressing, drug slow-release carrier materials, in-vivo imaging materials, detection analysis materials and the like, so that the application of the multifunctional mulberry silk fibroin as and/or in preparing daily chemical materials and/or biomedical materials and/or foods is within the protection scope of the application.
Preferably, the biomedical material comprises one or more of a biological dressing, a drug sustained release carrier material, an in vivo imaging material, and a detection analysis material.
Preferably, the daily chemical material includes, but is not limited to, a sunscreen skin care product and a whitening skin care product.
The invention has the following beneficial effects:
1. according to the invention, after a series of treatments are carried out on silkworm cocoons obtained by feeding quercetin to silkworms, the obtained multifunctional mulberry silk fibroin has good biocompatibility and biodegradability of the mulberry silk fibroin, has good oxidation resistance and ultraviolet absorption performance, can emit bright yellow-green light under the excitation of 365nm laser, has high-intensity fluorescence performance, can be clinically used for marking and tracking, and can be prepared into biomedical materials with good performance, such as biological dressing, drug slow-release carrier materials, in-vivo imaging materials, detection and analysis materials and the like.
2. The method adopts the feeding method to carry out functional modification on the mulberry silk fibroin, is simple and efficient, saves energy, protects environment, has high biological safety and is beneficial to large-scale production.
Drawings
FIG. 1 is a graph showing the results of measuring the oxidation resistance of the multifunctional mulberry silk sericin obtained in the blank group and examples 1 to 3.
FIG. 2 is an ultraviolet absorption curve of the multifunctional mulberry silk fibroin obtained in examples 1 to 3 and blank group in UVA 320-400 nm band.
FIG. 3 is a photograph of a blank group and the multifunctional mulberry silk sericin obtained in examples 1 to 3 under sunlight.
FIG. 4 is a photograph of a blank and the multifunctional mulberry silk sericin obtained in examples 1 to 3 in sunlight after being prepared into a solution.
FIG. 5 is a fluorescence chart of a blank and multifunctional mulberry silk sericin obtained by freeze-drying in examples 1 to 3 at an excitation wavelength of 365 nm.
In the figure, control represents a blank group (fed with mulberry leaves without quercetin suspension); QR-1.0% represents a mass ratio of quercetin to mulberry leaf of 1:100, i.e. the multifunctional mulberry silk fibroin obtained in example 1; QR-2.5% represents a mass ratio of quercetin to mulberry leaf of 2.5:100, i.e. the multifunctional mulberry silk sericin obtained in example 2; QR-5% represents a mass ratio of quercetin to mulberry leaf of 5:100, i.e. the multifunctional mulberry silk fibroin obtained in example 3.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Reagents and materials used in the following examples are commercially available unless otherwise specified.
Silkworm is from silk science and technology system of university of agricultural university of south China.
Quercetin, available from Hedera helix Biotechnology Co.
Example 1A multifunctional Mulberry Silk mucin
The multifunctional mulberry silk sericin is prepared by the following method:
feeding mulberry leaves sprayed with 0.3g/mL quercetin suspension on the surface of the mulberry leaves into silkworms of the third day of five ages (the dosage of the quercetin is controlled to be unchanged in the feeding stage, meanwhile, the silkworms can eat freely), and the mass ratio of the quercetin to the mulberry leaves is controlled to be 1:100, removing silkworm chrysalis in the silkworm cocoons obtained by spinning the silkworms to obtain cocoon shells; soaking cocoon shells in distilled water, heating for 4 hours at 60 ℃, vacuum-filtering with 400-mesh filter cloth, and finally drying filtrate at-50 ℃ for 60 hours to obtain the multifunctional mulberry silk fibroin.
Example 2A multifunctional Mulberry Silk mucin
The multifunctional mulberry silk sericin is prepared by the following method:
feeding mulberry leaves sprayed with 0.33g/mL quercetin suspension on the surface of the mulberry leaves with the total mass ratio of quercetin to mulberry leaves of 2.5, wherein the dosage of quercetin is controlled to be unchanged in the feeding stage and the mulberry leaves can be fed freely in the feeding stage for the third day: 100, removing silkworm chrysalis in the silkworm cocoons obtained by spinning the silkworms to obtain cocoon shells; soaking cocoon shells in distilled water, heating at 90 ℃ for 1h, vacuum-filtering with 400-mesh filter cloth, and finally drying filtrate at-55 ℃ for 50h to obtain the multifunctional mulberry silk fibroin.
Example 3A multifunctional Mulberry Silk mucin
The multifunctional mulberry silk sericin is prepared by the following method:
feeding mulberry leaves sprayed with 0.5g/mL quercetin suspension on the surface of the mulberry leaves into silkworms of the third day of five ages (the dosage of the quercetin is controlled to be unchanged in the feeding stage, meanwhile, the silkworms can eat freely), and the mass ratio of the quercetin to the mulberry leaves is controlled to be 5:100, removing silkworm chrysalis in the silkworm cocoons obtained by spinning the silkworms to obtain cocoon shells; soaking cocoon shells in distilled water, heating at 100deg.C for 4 hr, vacuum filtering with 500 mesh filter cloth, and drying the filtrate at-70deg.C for 40 hr to obtain the multifunctional mulberry silk sericin.
Experimental example 1 measurement of antioxidant Properties
Detection kit based on total antioxidant capacity (limited biotechnology produced from NanjingCompany) instruction, the oxidation resistance of the multifunctional mulberry silk sericin obtained in examples 1 to 3 and the mulberry silk sericin obtained in a blank group (feeding mulberry leaves without quercetin suspension) were measured using a total oxidation resistance detection kit. The main principle of the kit is as follows: the antioxidant substance can make Fe 3+ Reduction to Fe 2+ The latter can form firm complex with phenanthroline substance, then water bath is carried out for 30min at 37 ℃, then the absorbance is measured at 520nm, and the total antioxidant capacity of the blank group and the multifunctional mulberry silk sericin obtained in examples 1-3 can be obtained by calculation according to the result of absorbance.
The measurement results are shown in fig. 1, and as can be seen from fig. 1, the antioxidant capacities of the multifunctional mulberry silk sericin obtained in examples 1-3 are all significantly better than those of the blank group, which indicates that the multifunctional mulberry silk sericin obtained by feeding quercetin to silkworms and carrying out a series of treatments on cocoons obtained by spinning has better antioxidant performance. And when the mass ratio of quercetin to mulberry leaf is 5:100 In the case of example 3, the oxidation resistance of the multifunctional mulberry silk fibroin was optimized.
Experimental example 2 measurement of ultraviolet absorption Property
The ultraviolet absorption properties of the multifunctional mulberry silk sericin obtained in examples 1 to 3 and the mulberry silk sericin obtained in the blank group (feeding mulberry leaves without quercetin suspension) were measured by using an ultraviolet-visible spectrophotometer (UV-2550, shimadzu).
The ultraviolet absorption curve obtained by measurement is shown in figure 2, and as can be seen from figure 2, the ultraviolet absorption performance of the multifunctional mulberry silk fibroin obtained in examples 1-3 in the UVA 320-400 nm wave band is obviously better than that of the mulberry silk fibroin obtained in the blank group, so that the multifunctional mulberry silk fibroin obtained by feeding quercetin to mulberry silkworms, and after a series of treatments, the silkworm cocoons obtained by spinning the silkworm cocoons have better ultraviolet absorption performance. And when the mass ratio of quercetin to mulberry leaf is 2.5:100 (i.e., example 2), the ultraviolet absorption performance of the multifunctional mulberry silk fibroin was optimized.
Experimental example 3 determination of fluorescence Properties
The blank group and the multifunctional mulberry silk sericin obtained in examples 1 to 3 are respectively placed in sunlight for photographing, the photo is shown in fig. 3, and as can be seen from fig. 3, the blank group is in a milky white block shape in sunlight, and examples 1 to 3 are all in a pale yellow green block shape. Compared with the silkworm without quercetin, the silkworm fed with quercetin provided by the invention has the advantages that the color of the multifunctional mulberry silk sericin obtained after a series of treatments on silkworm cocoons obtained by spinning is obviously changed.
The blank group and the multifunctional mulberry silk fibroin obtained in examples 1-3 are respectively prepared into 5mg/mL multifunctional mulberry silk fibroin solution, the multifunctional mulberry silk fibroin solution is respectively photographed under sunlight, a photo is shown in fig. 4, the blank group is colorless, clear and transparent under sunlight, and examples 1-3 are all light green, clear and transparent. Compared with the silkworm without quercetin, the silkworm fed with quercetin provided by the invention has the advantages that the color of the multifunctional mulberry silk sericin obtained after a series of treatments on silkworm cocoons obtained by spinning is obviously changed.
The fluorescence of the blank group and the multifunctional mulberry silk fibroin obtained in examples 1-3 when excited at 365nm is observed in a UVP gel imaging system (BIO-RAD company, USA) respectively, the obtained fluorescence graph is shown in figure 5, and as can be seen from figure 5, the multifunctional mulberry silk fibroin in the blank group shows blue-white autofluorescence in the UVP gel imaging system, examples 1-3 show bright yellow-green fluorescence, and the obtained multifunctional mulberry silk fibroin emits bright yellow-green light under excitation of laser with 365nm wavelength after a series of treatments are carried out on silkworm silkworms, so that the multifunctional mulberry silk fibroin obtained by spinning the multifunctional mulberry silk fibroin has high-intensity fluorescence performance, can be clinically used for marking and tracking, and can be prepared into biomedical materials with better performance, such as biological dressings, drug slow release carrier materials, in-vivo imaging materials, detection analysis materials and the like.
In conclusion, after the silkworm cocoons obtained by feeding the quercetin to silkworms and spinning the silkworm cocoons are subjected to a series of treatments, the multifunctional mulberry silk fibroin obtained by the method has the advantages of good biocompatibility and biodegradability of the mulberry silk fibroin, good oxidation resistance and ultraviolet absorption, and high-intensity fluorescence, and can emit bright yellow-green light under excitation of 365nm laser, so that the multifunctional mulberry silk fibroin can be clinically used for marking and tracking, and can be prepared into biomedical materials with good performances, such as biological dressing, drug slow-release carrier materials, in-vivo imaging materials, detection analysis materials and the like.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (5)
1. A preparation method of multifunctional mulberry silk sericin is characterized in that mulberry leaves with quercetin suspension applied to the surfaces are used for feeding mulberry silkworms, and silkworm chrysalis in silkworm cocoons obtained by spinning the mulberry silkworms is removed to obtain cocoon shells; separating sericin from cocoon shells to obtain the multifunctional mulberry silk sericin; wherein, the mass ratio of quercetin to mulberry leaf is 2.5:100, wherein the concentration of the quercetin suspension is 0.3-0.5 g/mL; the mulberry silkworms are mulberry silkworms from the second day of five ages to the seventh day of five ages; the separation is that the cocoon shell is soaked in water and then heated for 1 to 4 hours at the temperature of between 60 and 100 ℃; the mass volume ratio of the cocoon shell to the water is 1g: 10-100 mL; filtering and freeze-drying are carried out after the separation; the freeze-drying is carried out for 40-60 h at the temperature of-70 to-50 ℃.
2. The multifunctional mulberry silk fibroin prepared by the method of claim 1.
3. The use of the multifunctional mulberry silk fibroin according to claim 2 as and/or for preparing daily chemical materials.
4. The use of the multifunctional mulberry silk fibroin of claim 2 in preparing biomedical materials.
5. The use according to claim 4, wherein the biomedical material comprises one or more of a biological dressing, a drug delivery carrier material, an in vivo imaging material, a detection analysis material.
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