CN114981642A - 基于图像分析液滴的方法、计算机装置及存储介质 - Google Patents

基于图像分析液滴的方法、计算机装置及存储介质 Download PDF

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CN114981642A
CN114981642A CN202080093583.0A CN202080093583A CN114981642A CN 114981642 A CN114981642 A CN 114981642A CN 202080093583 A CN202080093583 A CN 202080093583A CN 114981642 A CN114981642 A CN 114981642A
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image
dye channel
channel image
target image
working
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夏贇
席阳
赵霞
崔兴业
沈寒婕
陈芳
蒋慧
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MGI Tech Co Ltd
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MGI Tech Co Ltd
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
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  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Geometry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Processing (AREA)

Abstract

一种基于图像分析液滴的方法,通过获得液滴体系所对应的工作染料通道图像和参比染料通道图像(S1);分别对所述工作染料通道图像和参比染料通道图像作预设的图像处理操作(S2);将所述工作染料通道图像或者所述参比染料通道图像作为目标图像(S3),从该目标图像中确定每个液滴所在区域(S4);及基于所确定的每个液滴所在区域分析所述液滴体系(S5),可以有效提升液滴分析的准确性。还提供一种计算机装置及存储介质。

Description

PCT国内申请,说明书已公开。

Claims (20)

  1. PCT国内申请,权利要求书已公开。
CN202080093583.0A 2020-02-14 2020-02-14 基于图像分析液滴的方法、计算机装置及存储介质 Pending CN114981642A (zh)

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