CN114958820B - Water-soluble natural wax-dissolving agent and preparation method and application thereof - Google Patents
Water-soluble natural wax-dissolving agent and preparation method and application thereof Download PDFInfo
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- CN114958820B CN114958820B CN202210510298.5A CN202210510298A CN114958820B CN 114958820 B CN114958820 B CN 114958820B CN 202210510298 A CN202210510298 A CN 202210510298A CN 114958820 B CN114958820 B CN 114958820B
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- dissolving agent
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- soluble natural
- natural wax
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 91
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 33
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 33
- 239000002699 waste material Substances 0.000 claims abstract description 33
- 239000000243 solution Substances 0.000 claims abstract description 32
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000000661 sodium alginate Substances 0.000 claims abstract description 27
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 27
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 21
- 239000000706 filtrate Substances 0.000 claims abstract description 19
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 15
- 239000003094 microcapsule Substances 0.000 claims abstract description 15
- 239000003513 alkali Substances 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims abstract description 14
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 239000008162 cooking oil Substances 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 239000000725 suspension Substances 0.000 claims abstract description 9
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- 239000000919 ceramic Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 63
- 239000012188 paraffin wax Substances 0.000 claims description 25
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 24
- 239000001993 wax Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000000499 gel Substances 0.000 claims description 12
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 230000004913 activation Effects 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 7
- 238000007710 freezing Methods 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 6
- 239000008158 vegetable oil Substances 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 235000011187 glycerol Nutrition 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 235000013882 gravy Nutrition 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 239000003208 petroleum Substances 0.000 claims description 4
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- 239000010802 sludge Substances 0.000 claims description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 2
- 235000011152 sodium sulphate Nutrition 0.000 claims description 2
- 238000007711 solidification Methods 0.000 claims description 2
- 230000008023 solidification Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 5
- 230000002829 reductive effect Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 25
- 235000019198 oils Nutrition 0.000 description 23
- 239000010779 crude oil Substances 0.000 description 19
- 238000010411 cooking Methods 0.000 description 15
- 230000002265 prevention Effects 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 239000003129 oil well Substances 0.000 description 7
- 238000011056 performance test Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000012267 brine Substances 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 239000010806 kitchen waste Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000004519 grease Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- FYSSBMZUBSBFJL-UHFFFAOYSA-N 3-hydroxydecanoic acid Chemical compound CCCCCCCC(O)CC(O)=O FYSSBMZUBSBFJL-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 2
- -1 L-alpha-rhamnopyranosyl Chemical group 0.000 description 2
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 2
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000004695 Polyether sulfone Substances 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 1
- LLQHSBBZNDXTIV-UHFFFAOYSA-N 6-[5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-4,5-dihydro-1,2-oxazol-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC1CC(=NO1)C1=CC2=C(NC(O2)=O)C=C1 LLQHSBBZNDXTIV-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- AMAICRYCMCVAHT-UHFFFAOYSA-K calcium;sodium;trichloride Chemical compound [Na+].[Cl-].[Cl-].[Cl-].[Ca+2] AMAICRYCMCVAHT-UHFFFAOYSA-K 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000036978 cell physiology Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005660 hydrophilic surface Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000008723 osmotic stress Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
- C09K8/524—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning organic depositions, e.g. paraffins or asphaltenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/084—Polymers containing vinyl alcohol units
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
- C12R2001/385—Pseudomonas aeruginosa
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes
Abstract
The invention discloses a water-soluble natural wax-dissolving agent and a preparation method and application thereof, wherein the preparation steps of the wax-dissolving agent are as follows: (1) culturing pseudomonas aeruginosa in seed solution; (2) Preparing bacterial suspension from bacterial mud obtained after centrifuging the seed liquid; (3) The bacterial suspension reacts with a mixed solution composed of polyvinyl alcohol and calcium chloride and a sodium alginate solution to prepare PA/SA/PVA gel balls; (4) Solidifying the PA/SA/PVA gel ball to obtain PA/SA/PVA liquid core microcapsule; (5) preparing a culture solution; (6) Adding the liquid core microcapsule into a culture solution for fermentation to prepare a fermentation solution containing rhamnolipid; (7) passing the fermentation broth through a ceramic filter membrane to obtain a micro-filtrate; and (8) adding alkali and inorganic salt into the micro-filtrate. The water-soluble natural wax-dissolving agent provided by the invention has excellent wax-preventing performance, and the main raw materials of the wax-dissolving agent can be prepared by using the waste cooking oil as a fermentation carbon source, so that the production cost of the wax-dissolving agent is effectively reduced, and the wax-dissolving agent has the advantage of environmental protection.
Description
Technical Field
The invention belongs to the technical field of oilfield development, and particularly relates to a water-soluble natural wax-dissolving agent, and a preparation method and application thereof.
Background
In the process of crude oil exploitation, the temperature and the pressure of crude oil liquid are continuously reduced in the process of conveying the crude oil liquid from an oil well to the ground, wax contained in the crude oil liquid is usually crystallized and separated out, and the separated wax is continuously deposited on the sucker rod and the inner wall of an oil pipe; serious wax precipitation affects normal production operations of oil wells, and therefore, a great deal of effort is required to perform frequent well flushing and pump inspection operations to remove precipitated wax. The methods currently used for preventing and removing paraffin deposits in crude oil production mainly comprise the following three types: 1. a mechanical paraffin removal method, a thermal paraffin removal and chemical paraffin removal and prevention method, and a chemical paraffin removal and prevention method; the mechanical paraffin removal method and the thermal paraffin removal method are rarely adopted because the oil well has to be stopped when in operation and the energy consumption is larger; the chemical paraffin removal and prevention method has the advantages of convenient use, good application effect, no need of stopping production in the operation process and the like, and becomes the most common technology of paraffin removal and prevention of oil wells (Gao Wenyuan. Research on paraffin removal and prevention agents of oil wells [ D ]. Daqing petroleum institute, 2009.).
Chemical paraffin removal and inhibition generally refers to a method of adding paraffin solvents from an annular space to an oil pipe to prevent and remove paraffin deposits, and the method has the effects of reducing congealing, viscosity and blocking in addition to paraffin removal and inhibition (Chen Fu, qu Jinming, wang Fuxiang, etc. the current state of research and development of paraffin removal and inhibition agents for oil wells [ J ] oil and gas chemical industry, 2003, 32 (4): 243-245.). In recent years, with the increasing use of chemical paraffin removal and prevention methods, paraffin removal and prevention agents (also called paraffin dissolving agents) have been developed correspondingly. However, most of the main raw materials of the conventional paraffin removal and prevention agents are nondegradable surfactants artificially synthesized by petrochemical products, and the surfactants are required to be used in the use process by aromatic hydrocarbon solvents with strong toxicity such as benzene, dimethylbenzene and the like, so that the environment is damaged and the physical health of workers is damaged after long-term use (Dejian, and the like; the development and application of water-soluble and oil-soluble paraffin removal and prevention agents [ D ] Daqing petroleum institute, 2003.). Therefore, the design and synthesis of the green degradable nontoxic water-soluble wax dissolving method is very necessary and has a wide application prospect.
Disclosure of Invention
The invention aims to provide a water-soluble natural wax-dissolving agent, and a preparation method and application thereof.
In order to achieve the above object, the solution of the present invention is:
the preparation method of the water-soluble natural wax-dissolving agent is characterized by comprising the following steps:
(1) Inoculating pseudomonas aeruginosa on a nutrient gravy agar medium, performing constant-temperature activation, transferring the strain subjected to the constant-temperature activation into seed liquid, and performing shake flask culture to obtain seed liquid containing the pseudomonas aeruginosa;
(2) Centrifuging the seed solution prepared in the step (1) to remove supernatant, and adding the obtained bacterial mud into sterile physiological saline to prepare bacterial suspension;
(3) Adding the bacterial suspension prepared in the step (2) into a mixed solution composed of polyvinyl alcohol and calcium chloride, uniformly stirring, dropwise adding the obtained mixed solution into a sodium alginate solution, and filtering to obtain spherical sodium alginate/polyvinyl alcohol gel balls;
(4) Washing the sodium alginate/polyvinyl alcohol gel ball prepared in the step (3), then placing the washed gel ball into a calcium chloride solution for solidification, removing water on the surface of the solidified gel ball, and then freezing to prepare the sodium alginate/polyvinyl alcohol liquid core microcapsule;
(5) Adding a carbon source into an inorganic culture medium to prepare an inorganic salt culture solution containing the carbon source;
(6) Thawing the sodium alginate/polyvinyl alcohol liquid core microcapsule prepared in the step (4), adding the thawed sodium alginate/polyvinyl alcohol liquid core microcapsule into the inorganic salt culture solution containing carbon sources prepared in the step (5), and placing the inorganic salt culture solution into a fermentation tank for constant-temperature culture to prepare a rhamnolipid-containing fermentation liquor;
(7) Passing the rhamnolipid-containing fermentation liquor prepared in the step (6) through a ceramic filter membrane to remove larger proteins and insoluble impurities in the fermentation liquor, so as to prepare micro-filtrate;
(8) Adding alkali and inorganic salt into the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent.
Preferably, the constant temperature activation temperature in step (1) is 37 ℃; the constant temperature activation time is 24 hours.
Preferably, the mass ratio of polyvinyl alcohol to calcium chloride sodium alginate in the mixed solution of polyvinyl alcohol and calcium chloride in the step (3) is 1:1:2.
Preferably, the freezing temperature in the step (4) is-18 ℃ and the freezing time is 4-6h.
Preferably, the carbon source in step (5) is selected from one of waste cooking oil, glycerol or vegetable oil.
Preferably, the constant temperature culture temperature in the step (6) is 37 ℃ and the constant temperature culture time is 24 hours.
Preferably, the pore size of the ceramic filter membrane in the step (7) is 50 nm-1000 nm.
Preferably, the base in step (8) is one of ethanolamine or triethylamine; the inorganic salt is one of sodium sulfate or sodium chloride.
The water-soluble natural wax-dissolving agent prepared by the preparation method of the water-soluble natural wax-dissolving agent is characterized in that the mass ratio of rhamnolipid is 0.1-1.0%; the mass ratio of the alkali is 0.5 to 2.0 percent, the mass ratio of the inorganic salt is 0.1 to 1.0 percent, and the balance is water.
The water-soluble natural wax-dissolving agent prepared by the preparation method of the water-soluble natural wax-dissolving agent is applied to wax prevention and paraffin removal of petroleum pipelines.
Compared with the existing preparation method of wax-dissolving agent, the principle and gain effect of the invention are as follows:
1. according to the preparation method of the water-soluble natural wax-dissolving agent, in the process of preparing main raw materials of rhamnolipid, polyvinyl alcohol (PVA) and Sodium Alginate (SA) with good biocompatibility are selected as immobilization materials, and then an embedding method is used for immobilizing pseudomonas aeruginosa cells so as to prepare SA/PVA liquid core microencapsulated cells. The prepared liquid core microencapsulated cells can encapsulate microorganisms (such as pseudomonas aeruginosa) in an internal liquid environment through a layer of external capsule membrane so as to block the cells from entering the external environment, and the formed microcapsule membrane allows nutrients and microorganism products (such as rhamnolipid) to pass through bidirectionally; this not only facilitates the microorganisms to extract nutrients and release extracellular products, but also reduces the effects of product inhibition, thereby reducing the fermentation cycle and increasing the yield of extracellular products (Sun Z, lv G, li s.probing the role of microenvironment for microencapsulated sacchromyces cerevisiae under osmotic stress [ J ] Applied Microbial and Cell Physiology,2007, 75:1419-1427.).
2. The water-soluble natural wax-dissolving agent provided by the preparation method provided by the invention uses natural surfactant rhamnolipid as a main raw material and small amount of alkali and inorganic salt as auxiliary materials. The rhamnolipid in the wax-dissolving agent is used as a surfactant to enable the paraffin surface on the wall of the oil well to be reversed into a hydrophilic surface, so that the wax cannot be deposited on the surface continuously, and the effect of preventing the wax is achieved; in addition, the rhamnolipid is adsorbed on the surface of the oil pipe, so that the paraffin is beneficial to falling off from the surface of the pipe wall, and the effect of dissolving the paraffin is achieved. The addition of alkali and inorganic salts can further reduce the surface tension of rhamnolipids; therefore, when the wax-dissolving agent permeates into the gaps of the wax crystals of the loose structure, the binding force between wax molecules can be weakened, and the wax crystals are dispersed in the oil flow due to the disassembly.
3. The main raw material of the water-soluble natural wax-dissolving agent provided by the invention is degradable natural product rhamnolipid which is harmless to the environment, so that the sulfonate chemical synthesis surfactant which is high in toxicity and poor in biodegradability is successfully avoided.
4. The preparation method of the water-soluble natural wax-dissolving agent provided by the invention takes cooking waste oil or kitchen waste grease and the like as fermentation carbon sources to prepare and produce rhamnolipid, so that the production cost of the wax-dissolving agent is effectively reduced.
Drawings
FIG. 1 is a graph showing the recycling of PA/SA/PVA liquid core microcapsules.
Detailed Description
The present invention will be described in further detail with reference to examples. It is also to be understood that the following examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, and that the particular amounts of materials, reaction times and temperatures, process parameters, etc. shown are but one example of a suitable range, and that some insubstantial modifications and adaptations of the invention to those skilled in the art are within the scope of the invention.
The reagents and instrumentation used in the present invention require strict sterility before use.
Pseudomonas aeruginosa used in the invention is purchased from Beijing deposit biotechnology Co., ltd, and the strain deposit number is as follows:
ATCC 9027=dsm1128=ifo 13275 =nrrl-B-800; the strain was stored in a refrigerator at-80℃during the experiment.
The composition of the culture medium used in the invention is as follows:
nutrient gravy agar (g/L): 15.0 parts of agar powder; 3.0 parts of beef extract; peptone 10.0; 10.0% of NaCl; pH:7.0 to 7.2;
seed liquid (g/L): 3.0 parts of beef extract; peptone 10.0; 10.0% of NaCl; pH:7.0 to 7.2;
inorganic salt medium (g/L): naNO 3 6.0;NaCl 1.0;KCl 1.0;Na 2 HPO 4 0.3;KH 2 PO 4 3.0;MgSO 4 2.5;FeCl 3 0.16;CaCl 2 0.1;ZnSO 4 1.5;MnSO 4 1.5;CuSO 4 0.15、H 3 BO 3 1.5。
Example 1
The preparation method of the water-soluble natural wax-dissolving agent provided by the invention comprises the following steps:
(1) Inoculating pseudomonas aeruginosa (Pseudomonas aeruginosa, PA) on nutrient gravy agar medium, activating at constant temperature in a constant temperature box at 37 ℃ for 24 hours, transferring the strain subjected to constant temperature activation into 100mL of seed liquid (the inoculation amount is 5%), and culturing for 18 hours in shaking flask culture at 200rpm at 37 ℃ to obtain seed liquid containing pseudomonas aeruginosa;
(2) Centrifuging (12000 r/min,10 min) the seed solution prepared in the step (1) to remove supernatant, and washing the obtained bacterial sludge three times by using 2mL of 0.9% sterile physiological saline; adding the washed bacterial mud into 0.9% sterile physiological saline to prepare 5 g.L -1 Is a bacterial suspension of (a);
(3) Adding 25ml of the bacterial suspension prepared in the step (2) into a mixed solution consisting of polyvinyl alcohol (PVA) and calcium chloride under aseptic conditions, and uniformly stirring; wherein the concentration of PVA in the mixed solution is 6.0% w/v, caCl 2 The concentration of the mixture solution is 2.0% w/v, the mixture solution is sterilized for 30min at the high pressure of 121 ℃ before being used, then the mixture solution is cooled to room temperature for standby, and the concentration of the bacterial suspension after the mixture solution is added is 3.0% v/v; then the obtained mixed solution is added into 50ml of 1.0% w/v Sodium Alginate (SA) solution drop by drop under intense stirring, at the moment, the solution becomes turbid and rapidly forms gel balls, and spherical PA/SA/PVA gel balls are prepared after filtration;
(4) Washing the PA/SA/PVA gel beads prepared in the step (3) with 20ml of sterile water three times, and then adding the washed PA/SA/PVA gel beads into 50ml of 2.0% CaCl 2 Solidifying in the solution for 2.5h, absorbing the surface moisture, and freezing in a refrigerator at-18 ℃ for 4h to obtain the PA/SA/PVA liquid core microcapsule;
(5) Adding 1ml of cooking waste oil (soybean oil left after frying the deep-fried dough sticks, which is subjected to aseptic treatment after filtering before use) into 99ml of inorganic culture medium to prepare inorganic salt culture solution containing the cooking waste oil;
(6) Adding the PA/SA/PVA liquid core microcapsule prepared in the step (4) into the inorganic salt culture solution containing the waste cooking oil prepared in the step (5) according to the mass ratio of 5% after thawing, and then placing the inorganic salt culture solution into a fermentation tank to perform shaking culture at the constant temperature of 150r/min for 72 hours at the temperature of 37 ℃ to prepare a rhamnolipid-containing fermentation liquor;
(7) Passing the rhamnolipid-containing fermentation broth prepared in step (6) through a ceramic filter membrane (30 cm in length, 3cm in diameter, and 0.08m in filtration area) with a pore size of 500nm 2 ) Removing larger proteins and insoluble impurities in the fermentation liquor to obtain micro-filtrate; 2ml of the micro-filtrate was obtained by the anthrone sulfuric acid method (Neissa M, pinzon L, kwang J. Analysis of rhamnolipid biosurfactants by methylene blue complexation [ J)]Applied Microbiology and Biotechnology,2009, 82:975-981.) the total concentration of rhamnolipids contained was determined and the results are shown in table 1;
(8) Adding NaOH into the micro-filtrate prepared in the step (7) to adjust the pH value to 9.0, and then passing the obtained alkaline micro-filtrate through an ultrafiltration membrane element (PES polyethersulfone material, length of 30cm, diameter of 5cm and filtration area of 0.4 m) 2 ) Washing the filter membrane with 5ml water for 3 times after the filtration is finished, and collecting the filtrate to prepare rhamnolipid solution; taking 1ml of rhamnolipid solution for LC-MS analysis to determine the types and the contents of the rhamnolipids; the results of LC-MS analysis indicate that the rhamnolipid prepared by the method provided by the invention comprises the rhamnolipid Rha-C 10 -C 10 Rhamnolipid Rha-C 10 -C 10 Rhamnolipid Rha-C 10 -C 12 (or Rha-Rha-C) 12 -C 10 ) These three rhamnolipids. The structural formula of Rhamnolipid (Rhamnolipid) is shown as follows:
in the above formula: r in double rhamnose double lipid 1 Is L-alpha-rhamnopyranosyl, R 2 Beta-hydroxydecanoic acid; r in mono-rhamnose double lipid 1 Is H, R 2 Beta-hydroxydecanoic acid; r in double rhamnose monolipid 1 Is L-alpha-rhamnopyranosyl, R 2 Is H; r in monostearyl monoesters 1 Is H, R 2 H.
LC-MS analysis is to determine that the main product in the fermented solution is rhamnolipid, but the total rhamnolipid content is the most significant for the method provided by the invention; therefore, this step can be omitted in the following embodiments;
(9) And (3) adding 0.05g of triethylamine and 0.02g of NaCl into the rhamnolipid solution prepared in the step (8), and finally adding water to prepare 250ml of water-soluble natural wax-dissolving agent.
Referring to the Chinese oil and gas industry standard SY/T5370-1999) surface and interfacial tension determination method, using Texas-500C ultra-low interfacial tension meter, setting the rotation speed to 8000r/min and the temperature to 60 ℃ of the oil reservoir to determine the interfacial tension between the prepared water-soluble natural wax-dissolving agent and crude oil; the results are shown in Table 3.
Wax-proofing performance test reference (Xu Yanli, et al, study of water-soluble wax-proofing agent FL-3 and field test [ J ]. Petrochemical applications, 2013, 32 (4): 37-40.) for wax-soluble agents was conducted in indoor experiments using the "reverse bottle wax-proofing assay" to evaluate water-soluble wax-proofing agents; the method comprises the following specific steps: adding 250ml of crude oil with 30% of water content into a weighed brine bottle, adding 25mg of the wax-dissolving agent prepared in the step (9), putting the brine bottle in a water bath, keeping the temperature at 75 ℃ for 30min, taking out the brine bottle by hand, keeping the temperature at 60 ℃ for 2.5h, taking out the brine bottle, reversely buckling the brine bottle on the wide-mouth bottle at the temperature, keeping the temperature until the brine bottle does not drip oil, weighing and calculating, and finally comparing the sample with a blank sample without the wax-dissolving agent to calculate the wax-preventing rate of the sample, wherein the result is shown in Table 3.
Example 2
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: omitting the step (8), and directly fixing the volume of the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
Example 3
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 2ml, the inorganic culture medium is 98ml, the step (8) is omitted, and the micro-filtrate prepared in the step (7) is directly subjected to constant volume to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
Example 4
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 4ml, the inorganic culture medium is 96ml, the step (8) is omitted, and the micro-filtrate prepared in the step (7) is directly subjected to constant volume to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 1.
Example 5
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 6ml, the inorganic culture medium is 94ml, the step (8) is omitted, and the micro-filtrate prepared in the step (7) is directly subjected to constant volume to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 1.
TABLE 1 comparison of total rhamnolipid concentration at different waste cooking oil concentrations
Waste cooking oil concentration (v/v)%) | Total concentration of rhamnolipid (g/L) | |
Example 1 | 1 | 7.1 |
Example 3 | 2 | 15.3 |
Example 4 | 4 | 16.6 |
Example 5 | 6 | 17.8 |
It can be seen from Table 1 that the total concentration of rhamnolipids increases with increasing concentration of waste cooking oil, taking into account that the comprehensive utilization of the carbon source is optimal when the concentration of waste cooking oil is 2 v/v%.
Example 6
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is changed into waste glycerol (waste oil bath in a laboratory is subjected to filtration and aseptic treatment before use), the consumption of the waste glycerol is 2ml, and the consumption of the inorganic culture medium is 98ml; omitting the step (8), and directly fixing the volume of the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 2.
Example 7
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is changed into kitchen waste grease (sold in the market, and filtration and aseptic treatment are needed before use), the amount of the kitchen waste grease is 2ml, and the amount of the inorganic culture medium is 98ml; omitting the step (8), and directly fixing the volume of the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 2.
Example 8
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is changed into vegetable oil (commercial soybean oil which needs to be subjected to aseptic treatment before use), the vegetable oil dosage is 2ml, and the inorganic culture medium dosage is 98ml; omitting the step (8), and directly fixing the volume of the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 2.
Example 9
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is changed into waste gasoline (commercially available, which is subjected to aseptic treatment before use), wherein the consumption of the waste gasoline is 2ml, and the consumption of the inorganic culture medium is 98ml; omitting the step (8), and directly fixing the volume of the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the rest of the procedure is the same as in example 1. The results of the total concentration of rhamnolipid contained in step (7) are shown in table 2.
TABLE 2 comparison of total rhamnolipid concentrations using different carbon sources
Carbon source | Total concentration of rhamnolipid (g/L) | |
Example 3 | Waste cooking oil | 15.3 |
Example 6 | Waste glycerol | 8.2 |
Example 7 | Kitchen waste grease | 14.6 |
Example 8 | Vegetable oil | 15.8 |
Example 9 | Waste gasoline | 12.5 |
It can be seen from table 1 that the different carbon sources have a great influence on the total concentration of rhamnolipids, but the total concentration of rhamnolipids produced by fermentation of waste cooking oil, kitchen waste oil and vegetable oil as carbon sources is not greatly different. Therefore, the method provided by the invention can completely utilize the cooking waste oil and the kitchen waste grease to prepare the water-soluble wax-dissolving agent, thereby changing waste into valuable and reducing the production cost of the wax-dissolving agent.
Example 10
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 2ml, the inorganic culture medium is 98ml, the step (8) is omitted, and in the step (9), triethylamine is replaced by ethanolamine; the rest of the procedure is the same as in example 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
Example 11
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 2ml, the inorganic culture medium is 98ml, the step (8) is omitted, and in the step (9), triethylamine is replaced by ammonia water; the rest of the procedure is the same as in example 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
Example 12
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 2ml, the inorganic culture medium is 98ml, the step (8) is omitted, and in the step (9), triethylamine is replaced by sodium hydroxide solution; the rest of the procedure is the same as in example 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
Example 13
The procedure for the preparation of the wax-dissolving agent is described in reference to example 1, with the difference that: in the step (5), the cooking waste oil is 2ml, the inorganic culture medium is 98ml, the step (8) is omitted, and no alkali is added in the step (9); the rest of the procedure is the same as in example 1. The interfacial tension characterization procedure between the wax-dissolving agent and crude oil was the same as in example 1, and the results are shown in Table 3. The wax-dissolving agent obtained was subjected to the same wax-preventing performance test as in example 1, and the results are shown in Table 3.
TABLE 3 comparison of Water-soluble Natural wax-dissolving Agents Using different bases
Alkali | Interfacial tension (10) -3 mN/m) | Wax control rate (%) | |
Example 1 | Triethylamine | 20.1 | 67 |
Example 2 | Triethylamine | 19.6 | 65 |
Example 10 | Ethanolamine | 2.2 | 93 |
Example 11 | Ammonia water | 97.8 | 46 |
Example 12 | Sodium hydroxide | 90.5 | 49 |
Example 13 | N/A | 324.7 | 38 |
It can be seen from table 3 that the interfacial tension and wax-preventing rate of the water-soluble natural wax-dissolving agent provided by the invention are both affected by the type of the added alkali, wherein the effect of the used organic alkali is obviously better than that of inorganic alkali, and the wax-preventing rate can be as high as 93% when the ethanolamine is used as alkali. It should be noted that the results in example 2 show that the rhamnolipid fermentation broth prepared by the PA/SA/PVA liquid core microcapsule fermentation method provided by the present invention can be used for preparing the water-soluble natural wax dissolving agent by simply filtering with a ceramic filter membrane.
Example 14
Step for preparing wax-dissolving agent reference example 10. The wax-dissolving agent obtained was tested for wax-inhibiting properties by referring to example 1, except that: the test was performed using crude oil having a water content of 50%, and the results are shown in table 4.
Example 15
Step for preparing wax-dissolving agent reference example 10. The wax-dissolving agent obtained was tested for wax-inhibiting properties by referring to example 1, except that: the test was performed using crude oil having a water content of 60%, and the results are shown in table 4.
Example 16
Step for preparing wax-dissolving agent reference example 10. The wax-dissolving agent obtained was tested for wax-inhibiting properties by referring to example 1, except that: the test was performed using crude oil having a water content of 70%, and the results are shown in table 4.
TABLE 4 wax control of water soluble natural wax solubles in crude oils of varying water content
Crude oil moisture content (%) | Wax control rate (%) | |
Example 10 | 30 | 93 |
Example 14 | 50 | 95 |
Example 15 | 60 | 94 |
Example 16 | 70 | 94 |
From table 4, it can be seen that the interfacial wax control rate of the water-soluble natural wax-dissolving agent provided by the invention slightly increases to 94% or more with the increase of the water content of crude oil.
Example 17
The PA/SA/PVA core microcapsules used in example 3 were recovered and reused, and each use was cleaned with sterile water. The procedure for the preparation of rhamnolipids is as in example 1, repeated 9 times with the total concentration of rhamnolipids results shown in figure 1. As can be seen from fig. 1: after the PA/SA/PVA liquid core microcapsule provided by the invention is continuously cultured for 10 times, the total concentration of rhamnolipid in the obtained fermentation broth can still reach 8.3g/L, which shows that the microcapsule breakage number is small in the repeated experiment process.
Claims (7)
1. The preparation method of the water-soluble natural wax-dissolving agent comprises the steps of (by mass ratio) obtaining rhamnolipid in the water-soluble natural wax-dissolving agent of 0.1-1.0%; the mass ratio of the alkali is 0.5-2.0%, the mass ratio of the inorganic salt is 0.1-1.0%, and the balance is water; the method is characterized by comprising the following steps of:
(1) Inoculating pseudomonas aeruginosa on a nutrient gravy agar medium, performing constant-temperature activation, and transferring the strain subjected to the constant-temperature activation into a seed solution for culture to obtain a seed solution containing the pseudomonas aeruginosa;
(2) Adding the bacterial sludge obtained after removing the supernatant of the seed liquid prepared in the step (1) into sterile physiological saline to prepare bacterial suspension;
(3) Adding the bacterial suspension prepared in the step (2) into a mixed solution composed of polyvinyl alcohol and calcium chloride, stirring uniformly, then adding the obtained mixed solution into a sodium alginate solution dropwise, and filtering to obtain spherical sodium alginate/polyvinyl alcohol gel balls, wherein the mass ratio of polyvinyl alcohol, calcium chloride and sodium alginate in the mixed solution composed of polyvinyl alcohol and calcium chloride is 1:1:2;
(4) Washing the sodium alginate/polyvinyl alcohol gel ball prepared in the step (3), then placing the washed gel ball into a calcium chloride solution for solidification, removing water on the surface of the solidified gel ball, and then freezing to prepare the sodium alginate/polyvinyl alcohol liquid core microcapsule;
(5) Adding a carbon source into an inorganic culture medium to prepare an inorganic salt culture solution containing the carbon source;
(6) Adding the sodium alginate/polyvinyl alcohol liquid core microcapsule prepared in the step (4) into the inorganic salt culture solution containing carbon sources prepared in the step (5) for constant temperature culture to prepare fermentation liquor containing rhamnolipid;
(7) Passing the rhamnolipid-containing fermentation liquor prepared in the step (6) through a ceramic filter membrane to remove larger proteins and insoluble impurities in the fermentation liquor, so as to prepare micro-filtrate;
(8) Adding alkali and inorganic salt into the micro-filtrate prepared in the step (7) to prepare the water-soluble natural wax-dissolving agent; the alkali is one of ethanolamine or triethylamine; the inorganic salt is one of sodium sulfate or sodium chloride.
2. The method for preparing a water-soluble natural wax-dissolving agent according to claim 1, wherein the constant temperature activation temperature in the step (1) is 37 ℃; the constant temperature activation time was 24h.
3. The method for preparing a water-soluble natural wax-dissolving agent according to claim 1, wherein the freezing temperature in the step (4) is-18 ℃ and the freezing time is 4-6h.
4. The method for preparing a water-soluble natural wax-dissolving agent according to claim 1, wherein the carbon source in the step (5) is one selected from the group consisting of waste cooking oil, glycerin and vegetable oil.
5. The method for preparing a water-soluble natural wax-dissolving agent according to claim 1, wherein the constant temperature culture temperature in the step (6) is 37 ℃ and the constant temperature culture time is 24 hours.
6. The method for preparing a water-soluble natural wax-dissolving agent according to claim 1, wherein the pore diameter of the ceramic filter membrane in the step (7) is 50 nm-1000 nm.
7. Use of the water-soluble natural wax-dissolving agent prepared by the preparation method of the water-soluble natural wax-dissolving agent according to any one of claims 1 to 6 in wax control and paraffin removal of petroleum pipelines.
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