CN107189466A - A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt - Google Patents
A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt Download PDFInfo
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- C08L95/00—Compositions of bituminous materials, e.g. asphalt, tar, pitch
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- C08J2345/00—Characterised by the use of homopolymers or copolymers of compounds having no unsaturated aliphatic radicals in side chain, and having one or more carbon-to-carbon double bonds in a carbocyclic or in a heterocyclic ring system; Derivatives of such polymers
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Abstract
A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt, belong to pitch preparing technical field, including bombax cotton pretreatment, pseudomonas aeruginosa are inoculated in slant medium, are inoculated in seed culture medium, plasma mutagenesis, are inoculated in plating medium, are inoculated in fermentation medium, acetic acid extraction, prepare emulsified asphalt.Bombax cotton is modified, and can improve the physical and mechanical properties of pitch.Rhamnolipid has stronger surface-active and emulsifying capacity, can be degradable by biology, environmentally friendly.The pitch prepared using the present invention, emulsifying effectiveness is good, stability is good, with good filtration reduction and inhibition.Using plasma carries out mutagenic treatment as mutagens to pseudomonas aeruginosa strains, and the yield for obtaining rhamnolipid is higher.Using plasma is pre-processed to bombax cotton and other materials, with the bond strength of the compatibility and two phase constituents of reinforcing fiber and pitch.
Description
Technical field
The invention belongs to pitch preparing technical field, and in particular to a kind of bombax cotton is modified rhamnolipid emulsified asphalt
Preparation method.
Background technology
Because bituminous paving is steady, comfortable, the low advantage of noise, the construction that it is widely used in highway is worked as
In.However, as axle is carried and vehicle flowrate is continuously increased, the earlier period damage of bituminous paving is increasingly severe, therefore, it should pass through
Certain methods adjust bituminous concrete to improve pavement performance.In the modifying agent of these bituminous concretes, fiber is good because of its
Improvement result and its low advantage of simple in construction, cost obtained increasing concern.And at present on fibre modification
The research of pitch, need further development, particularly improve pitch mechanical performance and with fiber compatibility in terms of.
The content of the invention
According to problems of the prior art, have good mechanical performance and fiber compatible the invention provides a kind of
Property bombax cotton be modified rhamnolipid emulsified asphalt preparation method.
The present invention uses following technical scheme：
A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt, comprises the following steps：
Step one：10g bombax cottons are weighed, are put into the NaOH aqueous solution that 300mL, mass concentration are 8g/L, and put
Put in the thermostat water bath that temperature is 90 DEG C, alkali process 2h, then cleaned repeatedly to neutrality with deionized water；
Step 2：Bombax cotton after alkali process is added to the sodium chlorite aqueous solution that 200mL mass concentrations are 1%
In, pH value of solution is adjusted to 4.5 with acetic acid, 1h is handled at 8 DEG C, then with distilled water cyclic washing to neutrality, then at room temperature certainly
1d so is dried, is dried in the electric heating constant-temperature blowing drying box for being then placed into 60 DEG C to constant weight；
Step 3：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, culture
24h；
Step 4：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, training
Support 24h；
Step 5：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, put
Shake after 30min, filtered with the funnel for being plugged with sterile absorbent cotton in shaking table, draw 10mL filtrates in sterile petri dish, adopt
With atmospheric pressure at room plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is uniformly applied
It is distributed on plating medium, at 37 DEG C, flat board culture 4d；
Step 6：Bacterium colony on picking plating medium is placed in fermentation medium, and in 37 DEG C, 250rpm shaking table
After culture 4 days, zymotic fluid is obtained；
Step 7：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm draws supernatant with liquid-transfering gun
Liquid adds the acetic acid extraction of 5 times of volumes into container, is mixed with vortex oscillator, stands after 24h, upper organic phase is taken out
Mention in container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 8：By high-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, bombax cotton, rhamnolipid
According to mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and
Stirring mixing 20 minutes at a temperature of 150 DEG C；
Step 9：The mixture in reaction vessel is sheared by cutter, temperature is controlled in whole shear history
Degree is at 150 DEG C -160 DEG C, and the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in tiny
When particle is evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until high score
Untill sub- copolymer SBS is substantially dissolved in aromatic naphtha；
Step 10：After after each component dissolving fully, stop shearing, together put mixed solution into baking together with reaction vessel
Case, constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
It is preferred that, described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, albumen
Peptone 10g/L, sodium chloride 5g/L, agar 20g/L.
It is preferred that, described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、
NaCL1g/L、CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
It is preferred that, described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, phosphorus
Acid dihydride potassium 10g/L, disodium hydrogen phosphate 4g/L, agar 20g/L.
It is preferred that, described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
The beneficial effects of the present invention are：
1) bombax cotton is modified, and can improve the physical and mechanical properties of pitch.
2) rhamnolipid has stronger surface-active and emulsifying capacity, can be degradable by biology, environmentally friendly.
3) pitch prepared using the present invention, emulsifying effectiveness is good, stability is good, with good filtration reduction and suppression
Property.
4) using plasma carries out mutagenic treatment as mutagens to pseudomonas aeruginosa strains, obtains rhamnolipid
Yield it is higher.
5) using plasma is pre-processed to bombax cotton and other materials, with reinforcing fiber and the compatibility of pitch
With the bond strength of two phase constituents.
Embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt, comprises the following steps：
Step one：10g bombax cottons are weighed, are put into the NaOH aqueous solution that 300mL, mass concentration are 8g/L, and put
Put in the thermostat water bath that temperature is 90 DEG C, alkali process 2h, then cleaned repeatedly to neutrality with deionized water；
Step 2：Bombax cotton after alkali process is added to the sodium chlorite aqueous solution that 200mL mass concentrations are 1%
In, pH value of solution is adjusted to 4.5 with acetic acid, 1h is handled at 8 DEG C, then with distilled water cyclic washing to neutrality, then at room temperature certainly
1d so is dried, is dried in the electric heating constant-temperature blowing drying box for being then placed into 60 DEG C to constant weight；
Step 3：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, culture
24h；
Step 4：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, training
Support 24h；
Step 5：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, put
Shake after 30min, filtered with the funnel for being plugged with sterile absorbent cotton in shaking table, draw 10mL filtrates in sterile petri dish, adopt
With atmospheric pressure at room plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is uniformly applied
It is distributed on plating medium, at 37 DEG C, flat board culture 4d；
Step 6：Bacterium colony on picking plating medium is placed in fermentation medium, and in 37 DEG C, 250rpm shaking table
After culture 4 days, zymotic fluid is obtained；
Step 7：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm draws supernatant with liquid-transfering gun
Liquid adds the acetic acid extraction of 5 times of volumes into container, is mixed with vortex oscillator, stands after 24h, upper organic phase is taken out
Mention in container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 8：By high-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, bombax cotton, rhamnolipid
According to mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and
Stirring mixing 20 minutes at a temperature of 150 DEG C；
Step 9：The mixture in reaction vessel is sheared by cutter, temperature is controlled in whole shear history
Degree is at 150 DEG C -160 DEG C, and the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in tiny
When particle is evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until high score
Untill sub- copolymer SBS is substantially dissolved in aromatic naphtha；
Step 10：After after each component dissolving fully, stop shearing, together put mixed solution into baking together with reaction vessel
Case, constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
Described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, peptone 10g/L,
Sodium chloride 5g/L, agar 20g/L.
Described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、NaCL1g/
L、CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
Described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, biphosphate
Potassium 10g/L, disodium hydrogen phosphate 4g/L, agar 20g/L.
Described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
The storage stability of pitch made from the embodiment of the present invention 1 and other anionic emulsified bitumens on the market is contrasted
As a result 1 is see the table below, storage time is 5 days.
Table 1
Claims (5)
1. a kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt, it is characterised in that comprise the following steps：
Step one：10g bombax cottons are weighed, are put into the NaOH aqueous solution that 300mL, mass concentration are 8g/L, and be placed into
Temperature is in 90 DEG C of thermostat water bath, alkali process 2h, then is cleaned repeatedly to neutrality with deionized water；
Step 2：Bombax cotton after alkali process is added in the sodium chlorite aqueous solution that 200mL mass concentrations are 1%, used
Acetic acid adjusts pH value of solution to 4.5, and 1h is handled at 8 DEG C, is then dried naturally to neutrality, then at room temperature with distilled water cyclic washing
Dried in 1d, the electric heating constant-temperature blowing drying box for being then placed into 60 DEG C to constant weight；
Step 3：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, cultivates 24h；
Step 4：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, culture
24h；
Step 5：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, is placed in and shakes
In bed after concussion 30min, filtered with the funnel for being plugged with sterile absorbent cotton, 10mL filtrates are drawn in sterile petri dish, using normal
Pressure chamber isothermal plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is spread evenly across
On plating medium, at 37 DEG C, flat board culture 4d；
Step 6：Bacterium colony on picking plating medium is placed in fermentation medium, and is cultivated in 37 DEG C, 250rpm shaking table
After 4 days, zymotic fluid is obtained；
Step 7：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm is arrived with liquid-transfering gun Aspirate supernatant
In container, the acetic acid extraction of 5 times of volumes is added, is mixed with vortex oscillator, is stood after 24h, upper organic phase extracting is arrived
In container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 8：By high-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, bombax cotton, rhamnolipid according to
Mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and at 150 DEG C
At a temperature of stirring mixing 20 minutes；
Step 9：The mixture in reaction vessel is sheared by cutter, controls temperature to exist in whole shear history
150 DEG C -160 DEG C, the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in fine particle
When being evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until macromolecule is total to
Untill polymers SBS is substantially dissolved in aromatic naphtha；
Step 10：After after each component dissolving fully, stop shearing, together put mixed solution into baking oven together with reaction vessel,
Constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
2. a kind of bombax cotton according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature exists
In：Described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, peptone 10g/L, chlorination
Sodium 5g/L, agar 20g/L.
3. a kind of bombax cotton according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature exists
In：Described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、NaCL1g/L、
CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
4. a kind of bombax cotton according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature exists
In：Described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, potassium dihydrogen phosphate 10g/
L, disodium hydrogen phosphate 4g/L, agar 20g/L.
5. a kind of bombax cotton according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature exists
In：Described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
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| CN201710590099.9A CN107189466A (en) | 2017-07-19 | 2017-07-19 | A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt |
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| CN201710590099.9A CN107189466A (en) | 2017-07-19 | 2017-07-19 | A kind of bombax cotton is modified the preparation method of rhamnolipid emulsified asphalt |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958820A (en) * | 2022-05-11 | 2022-08-30 | 西南石油大学 | Water-soluble natural wax dissolving agent and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613725A (en) * | 2009-08-05 | 2009-12-30 | 河北鑫合生物化工有限公司 | Utilize microbial fermentation to prepare the method for rhamnolipid |
| CN104559267A (en) * | 2015-02-09 | 2015-04-29 | 中核华泰建设有限公司 | Preparation method of modified asphalt |
| CN105112032A (en) * | 2015-09-17 | 2015-12-02 | 西南石油大学 | Modified natural rock asphalt toughening agent and oil well cement paste system toughened through same |
| CN106367359A (en) * | 2016-11-16 | 2017-02-01 | 中国林业科学研究院林产化学工业研究所 | Aspergillus niger and application thereof to preparing citric acid from fermented acorns |
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2017
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|---|---|---|---|---|
| CN101613725A (en) * | 2009-08-05 | 2009-12-30 | 河北鑫合生物化工有限公司 | Utilize microbial fermentation to prepare the method for rhamnolipid |
| CN104559267A (en) * | 2015-02-09 | 2015-04-29 | 中核华泰建设有限公司 | Preparation method of modified asphalt |
| CN105112032A (en) * | 2015-09-17 | 2015-12-02 | 西南石油大学 | Modified natural rock asphalt toughening agent and oil well cement paste system toughened through same |
| CN106367359A (en) * | 2016-11-16 | 2017-02-01 | 中国林业科学研究院林产化学工业研究所 | Aspergillus niger and application thereof to preparing citric acid from fermented acorns |
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| 杨继生: "《表面活性剂原理与应用》", 31 December 2012, 东南大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958820A (en) * | 2022-05-11 | 2022-08-30 | 西南石油大学 | Water-soluble natural wax dissolving agent and preparation method and application thereof |
| CN114958820B (en) * | 2022-05-11 | 2023-10-31 | 西南石油大学 | Water-soluble natural wax-dissolving agent and preparation method and application thereof |
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