CN107189467A - A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt - Google Patents

A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt Download PDF

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Publication number
CN107189467A
CN107189467A CN201710590211.9A CN201710590211A CN107189467A CN 107189467 A CN107189467 A CN 107189467A CN 201710590211 A CN201710590211 A CN 201710590211A CN 107189467 A CN107189467 A CN 107189467A
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medium
modified
rhamnolipid
lignin fibre
emulsified asphalt
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章云
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Wuhu Kaiaoer Environmental Science and Technology Co Ltd
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Wuhu Kaiaoer Environmental Science and Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L95/00—Compositions of bituminous materials, e.g. asphalt, tar, pitch
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/28—Treatment by wave energy or particle radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2345/00—Characterised by the use of homopolymers or copolymers of compounds having no unsaturated aliphatic radicals in side chain, and having one or more carbon-to-carbon double bonds in a carbocyclic or in a heterocyclic ring system; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2401/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
    • C08J2401/02—Cellulose; Modified cellulose
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2201/00—Properties
    • C08L2201/06—Biodegradable
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00—Polymer mixtures characterised by other features
    • C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
    • C08L2205/035—Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00—Polymer mixtures characterised by other features
    • C08L2205/14—Polymer mixtures characterised by other features containing polymeric additives characterised by shape
    • C08L2205/16—Fibres; Fibrils

Abstract

A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt, belong to pitch preparing technical field, including prepared by lignin fibre, pseudomonas aeruginosa is inoculated in slant medium, is inoculated in seed culture medium, plasma mutagenesis, is inoculated in plating medium, is inoculated in fermentation medium, acetic acid extraction, prepares emulsified asphalt.Lignin fibre is modified, and can improve the physical and mechanical properties of pitch.Rhamnolipid has stronger surface-active and emulsifying capacity, can be degradable by biology, environmentally friendly.The pitch prepared using the present invention, emulsifying effectiveness is good, stability is good, with good filtration reduction and inhibition.Using plasma carries out mutagenic treatment as mutagens to pseudomonas aeruginosa strains, and the yield for obtaining rhamnolipid is higher.Using plasma is pre-processed to lignin fibre and other materials, with the bond strength of the compatibility and two phase constituents of reinforcing fiber and pitch.

Description

A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt
Technical field
The invention belongs to pitch preparing technical field, and in particular to a kind of lignin fibre is modified rhamnolipid emulsified asphalt Preparation method.
Background technology
Because bituminous paving is steady, comfortable, the low advantage of noise, the construction that it is widely used in highway is worked as In.However, as axle is carried and vehicle flowrate is continuously increased, the earlier period damage of bituminous paving is increasingly severe, therefore, it should pass through Certain methods adjust bituminous concrete to improve pavement performance.In the modifying agent of these bituminous concretes, fiber is good because of its Improvement result and its low advantage of simple in construction, cost obtained increasing concern.And at present on fibre modification The research of pitch, need further development, particularly improve pitch mechanical performance and with fiber compatibility in terms of.
The content of the invention
According to problems of the prior art, have good mechanical performance and fiber compatible the invention provides a kind of Property lignin fibre be modified rhamnolipid emulsified asphalt preparation method.
The present invention uses following technical scheme：
A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt, comprises the following steps：
Step one：Pulping back liquor powder is dissolved in the solution for being made into that mass concentration is 10% in suitable quantity of water, with a slow speed Filter paper carries out filtration treatment, backward filtrate in add the concentrated hydrochloric acid that concentration is 6mol/L, be stirred continuously until pH is 2, then stir 30min is mixed, obtained solution is filtered with slow filter paper, takes filter paper upper strata insoluble matter to be dried in 50 DEG C of vacuum drying chambers, Obtain alkali lignin powder；
Step 2：By alkali lignin powder and polyethylene glycol in mass ratio 1:3 are mixed, and 160 are put into after stirring DEG C oil bath pan in heat 2h, after heating is finished, pour into 1000mL pure water, stirring 2h filterings, and to filter paper upper strata insoluble matter Matter is dried in vacuo, and obtains modified alkali lignin powder；
Step 3：Modified alkali lignin powder is added in melt spinning machine, wood is prepared using melt-spinning technology spinning Quality fiber, wherein melt-spinning technology condition is：228 DEG C of temperature, receive silk speed 56m/min, spin duct oral pore footpath 0.8mm, The spinning mouth of pipe is away from receipts roll dies apart from 100mm；
Step 4：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, culture 24h；
Step 5：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, training Support 24h；
Step 6：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, put Shake after 30min, filtered with the funnel for being plugged with sterile absorbent cotton in shaking table, draw 10mL filtrates in sterile petri dish, adopt With atmospheric pressure at room plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is uniformly applied It is distributed on plating medium, at 37 DEG C, flat board culture 4d；
Step 7：Bacterium colony on picking plating medium is placed in fermentation medium, and in 37 DEG C, 250rpm shaking table After culture 4 days, zymotic fluid is obtained；
Step 8：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm draws supernatant with liquid-transfering gun Liquid adds the acetic acid extraction of 5 times of volumes into container, is mixed with vortex oscillator, stands after 24h, upper organic phase is taken out Mention in container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 9：By high-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, lignin fibre, rhamnose Fat is according to mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and Stirring mixing 20 minutes at a temperature of 150 DEG C；
Step 10：The mixture in reaction vessel is sheared by cutter, temperature is controlled in whole shear history Degree is at 150 DEG C -160 DEG C, and the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in tiny When particle is evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until high score Untill sub- copolymer SBS is substantially dissolved in aromatic naphtha；
Step 11：After after each component dissolving fully, stop shearing, together put mixed solution into baking together with reaction vessel Case, constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
It is preferred that, described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, albumen Peptone 10g/L, sodium chloride 5g/L, agar 20g/L.
It is preferred that, described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、 NaCL1g/L、CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
It is preferred that, described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, phosphorus Acid dihydride potassium 10g/L, disodium hydrogen phosphate 4g/L, agar 20g/L.
It is preferred that, described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
The beneficial effects of the present invention are：
1) lignin fibre is modified, and can improve the physical and mechanical properties of pitch.
2) rhamnolipid has stronger surface-active and emulsifying capacity, can be degradable by biology, environmentally friendly.
3) pitch prepared using the present invention, emulsifying effectiveness is good, stability is good, with good filtration reduction and suppression Property.
4) using plasma carries out mutagenic treatment as mutagens to pseudomonas aeruginosa strains, obtains rhamnolipid Yield it is higher.
5) using plasma is pre-processed to lignin fibre and other materials, with the compatible of reinforcing fiber and pitch The bond strength of property and two phase constituents.
Embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt, comprises the following steps：
Step one：Pulping back liquor powder is dissolved in the solution for being made into that mass concentration is 10% in suitable quantity of water, with a slow speed Filter paper carries out filtration treatment, backward filtrate in add the concentrated hydrochloric acid that concentration is 6mol/L, be stirred continuously until pH is 2, then stir 30min is mixed, obtained solution is filtered with slow filter paper, takes filter paper upper strata insoluble matter to be dried in 50 DEG C of vacuum drying chambers, Obtain alkali lignin powder；
Step 2：By alkali lignin powder and polyethylene glycol in mass ratio 1:3 are mixed, and 160 are put into after stirring DEG C oil bath pan in heat 2h, after heating is finished, pour into 1000mL pure water, stirring 2h filterings, and to filter paper upper strata insoluble matter Matter is dried in vacuo, and obtains modified alkali lignin powder；
Step 3：Modified alkali lignin powder is added in melt spinning machine, wood is prepared using melt-spinning technology spinning Quality fiber, wherein melt-spinning technology condition is：228 DEG C of temperature, receive silk speed 56m/min, spin duct oral pore footpath 0.8mm, The spinning mouth of pipe is away from receipts roll dies apart from 100mm；
Step 4：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, culture 24h；
Step 5：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, training Support 24h；
Step 6：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, put Shake after 30min, filtered with the funnel for being plugged with sterile absorbent cotton in shaking table, draw 10mL filtrates in sterile petri dish, adopt With atmospheric pressure at room plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is uniformly applied It is distributed on plating medium, at 37 DEG C, flat board culture 4d；
Step 7：Bacterium colony on picking plating medium is placed in fermentation medium, and in 37 DEG C, 250rpm shaking table After culture 4 days, zymotic fluid is obtained；
Step 8：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm draws supernatant with liquid-transfering gun Liquid adds the acetic acid extraction of 5 times of volumes into container, is mixed with vortex oscillator, stands after 24h, upper organic phase is taken out Mention in container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 9：By high-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, lignin fibre, rhamnose Fat is according to mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and Stirring mixing 20 minutes at a temperature of 150 DEG C；
Step 10：The mixture in reaction vessel is sheared by cutter, temperature is controlled in whole shear history Degree is at 150 DEG C -160 DEG C, and the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in tiny When particle is evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until high score Untill sub- copolymer SBS is substantially dissolved in aromatic naphtha；
Step 11：After after each component dissolving fully, stop shearing, together put mixed solution into baking together with reaction vessel Case, constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
Described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, peptone 10g/L, Sodium chloride 5g/L, agar 20g/L.
Described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、NaCL1g/ L、CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
Described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, biphosphate Potassium 10g/L, disodium hydrogen phosphate 4g/L, agar 20g/L.
Described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
The storage stability of pitch made from the embodiment of the present invention 1 and other anionic emulsified bitumens on the market is contrasted As a result 1 is see the table below, storage time is 5 days.
Table 1

Claims (5)

1. a kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt, it is characterised in that comprise the following steps：
Step one：Pulping back liquor powder is dissolved in the solution for being made into that mass concentration is 10% in suitable quantity of water, slow filter paper is used Carry out filtration treatment, backward filtrate in add concentration be 6mol/L concentrated hydrochloric acid, be stirred continuously until pH be 2, be stirred for 30min, obtained solution is filtered with slow filter paper, is taken filter paper upper strata insoluble matter to be dried in 50 DEG C of vacuum drying chambers, is obtained To alkali lignin powder；
Step 2：By alkali lignin powder and polyethylene glycol in mass ratio 1:3 are mixed, and 160 DEG C are put into after stirring 2h is heated in oil bath pan, after heating is finished, 1000mL pure water, stirring 2h filterings is poured into, and filter paper upper strata insoluble matter is entered Row vacuum drying, obtains modified alkali lignin powder；
Step 3：Modified alkali lignin powder is added in melt spinning machine, lignin is prepared using melt-spinning technology spinning Fiber, wherein melt-spinning technology condition is：228 DEG C of temperature, receipts silk speed 56m/min, spin duct oral pore footpath 0.8mm, spinning The mouth of pipe is away from receipts roll dies apart from 100mm；
Step 4：Pseudomonas aeruginosa is inoculated on slant medium, is placed in incubator, 37 DEG C of cultivation temperature, cultivates 24h；
Step 5：Bacterium colony on slant medium is connected to seed culture medium, 37 DEG C, shaking speed 200rmin-1, culture 24h；
Step 6：The bacterium colony on seed culture medium is gently blown into the container equipped with 50L physiological saline using transfer needle, is placed in and shakes In bed after concussion 30min, filtered with the funnel for being plugged with sterile absorbent cotton, 10mL filtrates are drawn in sterile petri dish, using normal Pressure chamber isothermal plasma mutagenic treatment 2min, then using normal saline dilution to 8% (v/v), dilution is spread evenly across On plating medium, at 37 DEG C, flat board culture 4d；
Step 7：Bacterium colony on picking plating medium is placed in fermentation medium, and is cultivated in 37 DEG C, 250rpm shaking table After 4 days, zymotic fluid is obtained；
Step 8：Zymotic fluid is taken, the centrifugal treating 3min under conditions of 25 DEG C, 10000rpm is arrived with liquid-transfering gun Aspirate supernatant In container, the acetic acid extraction of 5 times of volumes is added, is mixed with vortex oscillator, is stood after 24h, upper organic phase extracting is arrived In container, and put it into ventilating kitchen and treat that ether volatilization is complete, obtain rhamnolipid；
Step 9：High-molecular copolymer SBS, terpene resin, Plasticizer DBP, aromatic naphtha, lignin fibre, rhamnolipid are pressed According to mass ratio 11:21:4:9:3:1 adds in reaction vessel, using atmospheric pressure at room plasma mutagenic treatment 3min, and 150 Stirring mixing 20 minutes at a temperature of DEG C；
Step 10：The mixture in reaction vessel is sheared by cutter, controls temperature to exist in whole shear history 150 DEG C -160 DEG C, the rotating speed of cutter keeps 3500r/min, shears 5-10min, when high-molecular copolymer SBS is in fine particle When being evenly distributed in aromatic naphtha, 6500r/min THE ADIABATIC SHEAR IN 20min are arrived in the rotating speed lifting of cutter, until macromolecule is total to Untill polymers SBS is substantially dissolved in aromatic naphtha；
Step 11：After after each component dissolving fully, stop shearing, together put mixed solution into baking oven together with reaction vessel, Constant temperature develops 2h under the conditions of 170 DEG C, produces pitch.
2. a kind of lignin fibre according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature It is：Described slant medium is beef extract-peptone agar medium, comprising：Beef extract 3g/L, peptone 10g/L, chlorine Change sodium 5g/L, agar 20g/L.
3. a kind of lignin fibre according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature It is：Described fermentation medium is included：K2HPO44g/L、KH2PO44g/L、KCL1g/L、NaNO32.5g/L、NaCL1g/L、 CaCl2·2H2O 0.1g/L, MgSO40.2g/L, dusty yeast 1g/L, glycerine 30v/v.
4. a kind of lignin fibre according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature It is：Described plating medium is included：Glucose 20g/L, ammonium nitrate 2.5g/L, yeast extract 0.05g/L, potassium dihydrogen phosphate 10g/L, disodium hydrogen phosphate 4g/L, agar 20g/L.
5. a kind of lignin fibre according to claim 1 is modified the preparation method of rhamnolipid emulsified asphalt, its feature It is：Described seed culture medium is included：NaClg/L, tryptone 10g/L, dusty yeast 5g/L.
CN201710590211.9A 2017-07-19 2017-07-19 A kind of lignin fibre is modified the preparation method of rhamnolipid emulsified asphalt Pending CN107189467A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109137139A (en) * 2018-07-27 2019-01-04 北京林业大学 A kind of preparation method of lignin-base fiber

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101948786A (en) * 2010-09-03 2011-01-19 中国石油天然气股份有限公司 Pseudomonas aeruginosa for producing rhamnolipid with high yield and application thereof
CN104312942A (en) * 2014-09-25 2015-01-28 北京华纳斯科技有限公司 Preparation method of Pseudomonoas aeruginosa inoculant and rhamnolipid broth
CN104592768A (en) * 2013-10-31 2015-05-06 中国石油化工股份有限公司 Anionic emulsified asphalt and preparation method thereof
CN105315684A (en) * 2014-12-03 2016-02-10 重庆市六工新材料有限公司 Emulsified asphalt modified by carbon nanometer fiber and preparing method thereof
CN105567581A (en) * 2015-09-28 2016-05-11 中国石油化工股份有限公司 Pseudomonas aeruginosa DB high in rhamnolipid yield and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101948786A (en) * 2010-09-03 2011-01-19 中国石油天然气股份有限公司 Pseudomonas aeruginosa for producing rhamnolipid with high yield and application thereof
CN104592768A (en) * 2013-10-31 2015-05-06 中国石油化工股份有限公司 Anionic emulsified asphalt and preparation method thereof
CN104312942A (en) * 2014-09-25 2015-01-28 北京华纳斯科技有限公司 Preparation method of Pseudomonoas aeruginosa inoculant and rhamnolipid broth
CN105315684A (en) * 2014-12-03 2016-02-10 重庆市六工新材料有限公司 Emulsified asphalt modified by carbon nanometer fiber and preparing method thereof
CN105567581A (en) * 2015-09-28 2016-05-11 中国石油化工股份有限公司 Pseudomonas aeruginosa DB high in rhamnolipid yield and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109137139A (en) * 2018-07-27 2019-01-04 北京林业大学 A kind of preparation method of lignin-base fiber

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