CN114958714A - Pollution-free cell culture method and system - Google Patents

Pollution-free cell culture method and system Download PDF

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Publication number
CN114958714A
CN114958714A CN202210703417.9A CN202210703417A CN114958714A CN 114958714 A CN114958714 A CN 114958714A CN 202210703417 A CN202210703417 A CN 202210703417A CN 114958714 A CN114958714 A CN 114958714A
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culture
bottom frame
culture dish
air
contamination
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Chinese (zh)
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董银
何春兰
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
    • A61L9/145—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes air-liquid contact processes, e.g. scrubbing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
    • A61L9/18—Radiation
    • A61L9/20—Ultra-violet radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/16—Sterilization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
    • A61L2101/32—Organic compounds
    • A61L2101/34—Hydroxy compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The invention relates to the technical field of cell culture-free, in particular to a cell pollution-free culture method and a cell pollution-free culture system; the method comprises the following steps: the method comprises the following steps: placing the culture dish in a culture system, and sterilizing and purifying air around the culture dish through the culture system; step two: making the purified air flow around the culture dish; step three: adding the culture medium and the cells into a culture dish in sequence; step four: covering the cover on the culture dish and disinfecting a gap between the cover and the bottom of the culture dish by using a culture system; step five: transferring the culture dish into a sterile culture room for culture; the system comprises a bottom frame, an ultraviolet lamp A fixed on the outer side of the upper end surface of the bottom frame, a fan arranged in the bottom frame and used for enabling air to enter the bottom frame from the lower part, and an ultraviolet lamp B fixed on the inner wall of the bottom frame; can satisfy the requirement of operators to carry out pollution-free culture of cells at any time and any place.

Description

Pollution-free cell culture method and system
Technical Field
The invention relates to the technical field of cell culture, in particular to a pollution-free cell culture method and system.
Background
Cell culture refers to a method for simulating in-vivo environment in vitro to enable the in-vivo environment to survive, grow, reproduce and maintain main structures and functions, wherein non-toxicity and sterility are the primary conditions for culturing cells in vitro, and bacteria and toxic substances are required to be prevented from entering a culture dish for culturing cells;
therefore, the application provides a method and a system for pollution-free culture of cells, which can meet the requirement that operators can culture the cells at any time and any place, and can complete the pollution-free culture of the cells outside places such as a specific sterile room.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a method and a system for culturing cells without pollution, which can meet the requirement that operators culture the cells without pollution at any time and any place.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a method for culturing cells without pollution, which comprises the following steps:
the method comprises the following steps: placing the culture dish in a culture system, and sterilizing and purifying air around the culture dish through the culture system;
step two: making the purified air flow to the periphery by taking the culture dish as a center;
step three: adding the culture medium and the cells into a culture dish in sequence;
step four: covering the cover on the culture dish and disinfecting a gap between the cover and the bottom of the culture dish by using a culture system;
step five: the culture dish was transferred to a sterile culture room for culturing.
The system further comprises a bottom frame, an ultraviolet lamp A fixed on the outer side of the upper end surface of the bottom frame, a fan installed in the bottom frame and used for enabling air to enter the bottom frame from the lower part, and an ultraviolet lamp B fixed on the inner wall of the bottom frame.
The system further comprises a net which can be embedded into the bottom frame and is uniformly provided with air outlets at the periphery, and a filtering membrane which is fixed below the net and is used for filtering bacteria.
Further the system still including fixing the base at the underframe lower extreme, and demountable installation cotton net in the base, and fix on the base and be connected with the workbin of a plurality of nozzles, a plurality of nozzles are located the base.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a flow chart of a contamination-free cell culture method according to the present invention;
FIG. 2 is a schematic view showing the construction of a culture system according to the present invention;
FIG. 3 is a schematic structural view of a bottom frame of the present invention;
FIG. 4 is a schematic structural view of a blower according to the present invention;
FIG. 5 is a schematic view of the structure of the mesh and step grooves of the present invention;
FIG. 6 is a schematic view showing the structure of a filtration membrane and a filter paper according to the present invention;
FIG. 7 is a schematic view showing a structure of a part of a culture system according to the present invention;
FIG. 8 is a schematic view of the construction of the base and cotton web of the present invention;
FIG. 9 is a schematic view of the construction of the cotton web of the present invention;
FIG. 10 is a schematic view showing the construction of a post and a rotating arm according to the present invention;
fig. 11 is a schematic view showing the structure of a rotating arm and a screw in the present invention.
Detailed Description
A method for culturing cells without pollution, which comprises the following steps:
the method comprises the following steps: placing the culture dish in a culture system, and sterilizing and purifying air around the culture dish through the culture system;
step two: making the purified air flow around the culture dish;
step three: adding the culture medium and the cells into a culture dish in sequence;
step four: covering the cover on the culture dish and disinfecting a gap between the cover and the bottom of the culture dish by using a culture system;
step five: the culture dish was transferred to a sterile culture room for culturing.
By observing fig. 1, according to the process of the method for cell contamination-free culture shown in the figure, firstly, the culture dish itself is sterilized and disinfected, then the culture dish is processed and placed in the culture system, then the air around the culture dish is sterilized and purified by the culture system, and the purified air flows around the culture dish as the center, so that the air with bacteria is prevented from flowing into the culture dish from the periphery; then adding the culture medium and the cells into the culture dish in sequence; after the operation is finished, the cover is covered on the culture dish, and a culture system is utilized to disinfect the gap between the cover and the bottom of the culture dish, so that bacteria are prevented from entering the culture dish through the gap between the cover and the bottom of the culture dish; finally, the culture dish is moved into a sterile culture room for culture;
the method can carry out pollution-free culture in a mode of killing bacteria in the surrounding air in the cell culture process, so that the hidden danger that the bacteria are mixed into a culture dish when the air flows can be avoided.
By observing fig. 3 and 4, one exemplary procedure by which air sterilization by ultraviolet light can be obtained is as follows:
the system comprises a tubular bottom frame 01, an ultraviolet lamp A02 with a self-contained power supply is arranged on the outer side of the upper end surface of the bottom frame 01, an ultraviolet lamp B04 with a self-contained power supply is arranged on the inner wall of the bottom frame 01, a fan 03 is arranged in the bottom frame 01, and air can penetrate through the bottom frame 01 from bottom to top when the fan 03 works; when air is sterilized, the fan 03 can drive the air to enter from the lower part of the bottom frame 01 and flow out from the upper part of the bottom frame 01 by penetrating through the bottom frame 01 only by enabling the fan 03 to work, and when the air passes through the ultraviolet lamp B04, the ultraviolet lamp B04 can kill bacteria in the air, so that the effect of sterilizing the bacteria in the air is realized;
meanwhile, an ultraviolet lamp A02 with a self-contained power supply is arranged on the outer side of the upper end surface of the bottom frame 01, the ultraviolet lamp A02 can form an annular sterilization range, and the periphery of the bottom frame 01 is covered to kill bacteria entering the air above the bottom frame 01 from the periphery, so that the bacteria cannot exist in the air around when cell culture is carried out above the bottom frame 01;
utilize fan 03's drive to form the malleation around the culture dish, make the sterile air around the culture dish constantly to flow to around, avoid the air flow direction culture dish of the peripheral area that has the bacterium to reach and avoid contaminated effect when can be for cultivateing the cell.
By observing fig. 5 to 7, one exemplary working process that can be obtained to further improve the sterilization effect according to the graph is:
the system is still including the net 05 that can imbed in the middle of the underframe 01, net 05 has the gas outlet to make in the underframe 01 air that upwards flows out upwards to flow out by the dispersion from net 05 all around upwards around by the equipartition around the net, thereby form an annular sky and flow the region, it is continuous upwards flowing to drive the air of net 05 top, thereby it does not have the bacterium to ensure in the air around the net 05, net 05 below fixedly connected with is used for filtering the filtration membrane 07 of bacterium, can enter into the air in the middle of the underframe 01 from underframe 01 below under the effect of fan 03 at first through filtration membrane 07, thereby utilize filtration membrane 07 tentatively to filter the bacterium in the air, thereby improved the degerming effect to the air.
By observing fig. 5-7, one exemplary process that may be derived to facilitate the placement of different types of culture plates from the illustration is:
the middle part of the net 05 is provided with a step groove 06, and the depth interval of the step groove 06 from outside to inside is increased; when the culture dish is placed, the culture dish is placed in the step groove 06, the culture dish can be clamped into a certain depth corresponding to the step groove 06 according to the size of the culture dish, so that the culture dish is clamped through the side wall of the step groove 06, the culture dish is prevented from accidentally sliding in the horizontal direction in the step groove 06, and the culture dish can be stably placed in the step groove 06 in the using process; the air flowing out through the net 05 can form a protective ring around the culture dish, so that the culture dish is positioned in a safe area at the center of the protective ring, and the air around the culture dish continuously flows upwards during operation, thereby preventing the air with bacteria from flowing into the culture dish.
By observing fig. 5-7, one exemplary operation that can be achieved for dust removal according to the figures is:
the lower end of the net 05 is fixed with filter paper 08 for filtering dust in air, and the filter paper 08 is positioned below the filter membrane 07; in the using process, air entering the bottom frame 01 can firstly pass through the filter paper 08, and the filter paper 08 can filter impurities and dust in the air, so that the air entering the bottom frame 01 is purified, and the dust is prevented from entering a culture dish.
By observing fig. 5-7, one exemplary operation that can be achieved for uniformly directing air according to the illustration is:
the lower end surface of the net 05 is a conical bottom 09 capable of guiding air flowing upwards to the periphery; when the air flows upwards and touches the conical bottom 09 on the lower end surface of the net 05, the air can be uniformly dispersed to the periphery through the guiding of the conical surface of the conical bottom 09, so that the sterilized air can uniformly flow out from the air outlet of the net 05.
By observing fig. 7-9, one exemplary operation that can be achieved for pre-sterilizing air according to the figures is:
the system also comprises a base 10 fixed at the lower end of the bottom frame 01, a cotton net 11 is detachably installed in the base 10, the outer wall of the base 10 is connected with a bin 12, a plurality of nozzles are uniformly distributed on the inner side of the bin 12 in the circumferential direction, the plurality of nozzles are positioned in the base 10, and the plurality of nozzles are positioned above the cotton net 11; when using, at first add workbin 12 works as well with alcohol or antiseptic solution that disinfects, the rethread is sprayed on cotton net 11 under the effect of force pump through a plurality of nozzles, and cotton net 11 makes the cotton material, can absorb alcohol or antiseptic solution, when the air flows the entering from base 10 below, can make the air tentatively pass through cotton net 11 to utilize alcohol or antiseptic solution in the cotton net 11 to carry out preliminary sterilization to the air, further ensure the bactericidal effect to the air.
By observing fig. 2 to 11, one exemplary working process that can be obtained by wiping the gap between the cap and the bottom of the culture dish is as follows:
the system also comprises a screw rod 15, a rotating sleeve 16 which is sleeved on the screw rod 15 and can rotate around the axis of the rotating sleeve, spiral threads matched with the screw rod 15 and a pressure plate 17 which slides at the lower end of the screw rod 15 are arranged on the inner wall of the rotating sleeve 16, a spring is fixedly connected between the screw rod 15 and the pressure plate 17, a side rod 18 which slides on the rotating sleeve 16 and is connected with a sponge, and a spring is fixedly connected between the side rod 18 and the rotating sleeve 16; after covering the lid on the culture dish, alright use 15 to drive the clamp plate 17 and press on the lid with the hob, can cushion clamp plate 17 under the effect of spring, avoid clamp plate 17 oppression lid, outwards stimulate side lever 18 simultaneously, the spring between side lever 18 and the commentaries on classics cover 16 is compressed this moment, after clamp plate 17 pressed on the lid, alright unclamp side lever 18 with unclamping, under the effect of the spring between side lever 18 and commentaries on classics cover 16 side lever 18 can make the sponge contact of fixing on it at culture dish and lid, add alcohol or antiseptic solution in advance in the middle of the sponge, last only need to rotate commentaries on classics cover 16, alright it is rotatory around the side of lid and culture dish to drive the sponge on the side lever 18 through changeing cover 16, thereby realize carrying out the disinfection's effect of disinfecting through alcohol or antiseptic solution in the sponge with lid and culture dish bottom gap department.
By observing fig. 2-11, one exemplary process that can be flexibly used according to the illustration in the figures is:
the system also comprises a vertical column 13 fixed on the bottom frame 01 and a rotating arm 14 rotating on the vertical column 13 through a torsion spring, the torsion spring drives the rotating arm 14 to be far away from the net 05 in a normal state so as to facilitate an operator to operate the culture dish, the spiral rod 15 can slide up and down on the rotating arm 14, after the cover is covered, the top end of the spiral rod 15 can be held, then the rotating arm 14 is enabled to rotate above the cover on the vertical column 13, at the moment, the torsion spring is stretched, then the spiral rod 15 is pressed downwards, the spiral rod 15 slides downwards on the rotating arm 14 and drives the rotating sleeve 16 to rotate through self spiral, and therefore, the side rod 18 can drive the sponge to wipe and sterilize the gap between the cover and the bottom of the culture dish when the spiral rod 15 descends to drive the pressing plate 17 to press the cover.
By observing fig. 10 and 11, one exemplary procedure by which the cap can be sterilized is as shown in the figures:
the lower end of the pressing plate 17 is connected with a spongy cushion capable of storing alcohol, and the cap can be disinfected by the alcohol in the spongy cushion when the pressing plate 17 presses the cap.

Claims (10)

1. A method for culturing cells without pollution, which is characterized by comprising the following steps:
the method comprises the following steps: placing the culture dish in a culture system, and sterilizing and purifying air around the culture dish through the culture system;
step two: making the purified air flow around the culture dish;
step three: adding the culture medium and the cells into a culture dish in sequence;
step four: covering the cover on the culture dish and disinfecting a gap between the cover and the bottom of the culture dish by using a culture system;
step five: the culture dish was transferred to a sterile culture room for culturing.
2. The contamination-free cell culture method according to claim 1, characterized in that: the system comprises a bottom frame (01), an ultraviolet lamp A (02) fixed on the outer side of the upper end face of the bottom frame (01), a fan (03) installed in the bottom frame (01) and used for enabling air to enter the bottom frame (01) from the lower side, and an ultraviolet lamp B (04) fixed on the inner wall of the bottom frame (01).
3. The contamination-free cell culture method according to claim 2, characterized in that: the system also comprises a net (05) which can be embedded into the bottom frame (01) and is uniformly provided with air outlets at the periphery, and a filtering membrane (07) which is fixed below the net (05) and is used for filtering bacteria.
4. The contamination-free cell culture method according to claim 3, characterized in that: the middle of the net (05) is provided with a step groove (06), and the depth interval of the step groove (06) from outside to inside is increased.
5. The contamination-free cell culture method according to claim 4, characterized in that: the lower end of the net (05) is fixed with filter paper (08) for filtering dust in air, and the filter paper (08) is positioned below the filter membrane (07).
6. The contamination-free cell culture method according to claim 5, characterized in that: the lower end surface of the net (05) is a conical bottom (09) capable of guiding air flowing upwards to the periphery.
7. The contamination-free cell culture method according to claim 6 or 5, characterized in that: the system also comprises a base (10) fixed at the lower end of the bottom frame (01), a cotton net (11) detachably installed in the base (10), and a material box (12) fixed on the base (10) and connected with a plurality of nozzles, wherein the nozzles are positioned in the base (10).
8. The contamination-free cell culture method according to claim 7, characterized in that: the system also comprises a screw rod (15), a rotating sleeve (16) which is sleeved on the screw rod (15) and can rotate around the axis of the rotating sleeve, helical threads matched with the screw rod (15) are arranged on the inner wall of the rotating sleeve (16), a pressing plate (17) which slides at the lower end of the screw rod (15), and a side rod (18) which slides on the rotating sleeve (16) and is connected with sponge.
9. The contamination-free cell culture method according to claim 8, characterized in that: the system also comprises a vertical column (13) fixed on the bottom frame (01) and a rotating arm (14) rotating on the vertical column (13) through a torsion spring, and the screw rod (15) can slide up and down on the rotating arm (14).
10. The contamination-free cell culture method according to claim 8, characterized in that: the lower end of the pressing plate (17) is connected with a spongy cushion capable of storing alcohol.
CN202210703417.9A 2022-06-21 2022-06-21 Pollution-free cell culture method and system Pending CN114958714A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210703417.9A CN114958714A (en) 2022-06-21 2022-06-21 Pollution-free cell culture method and system

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Application Number Priority Date Filing Date Title
CN202210703417.9A CN114958714A (en) 2022-06-21 2022-06-21 Pollution-free cell culture method and system

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CN114958714A true CN114958714A (en) 2022-08-30

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010029122A (en) * 1999-09-29 2001-04-06 복성해 Supplementary culture vessel for prevention of microbial contamination in plant and animal cell and tissue culture
US20150218503A1 (en) * 2012-09-26 2015-08-06 Hitachi, Ltd. Cell-culturing vessel and cell-culturing device using same
US20190316071A1 (en) * 2016-12-30 2019-10-17 Hektros S.r.l. Perfusion device
CN113832093A (en) * 2021-09-23 2021-12-24 周溪桥 Pollution-free cell culture method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010029122A (en) * 1999-09-29 2001-04-06 복성해 Supplementary culture vessel for prevention of microbial contamination in plant and animal cell and tissue culture
US20150218503A1 (en) * 2012-09-26 2015-08-06 Hitachi, Ltd. Cell-culturing vessel and cell-culturing device using same
US20190316071A1 (en) * 2016-12-30 2019-10-17 Hektros S.r.l. Perfusion device
CN113832093A (en) * 2021-09-23 2021-12-24 周溪桥 Pollution-free cell culture method

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