CN114948816A - Sun-screening and anti-photoaging traditional Chinese medicine composition and application thereof - Google Patents
Sun-screening and anti-photoaging traditional Chinese medicine composition and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a sun-screening anti-photoaging traditional Chinese medicine composition and application thereof, wherein the sun-screening anti-photoaging traditional Chinese medicine composition comprises effective components and DMSO (dimethylsulfoxide), wherein the effective components comprise a methanol reflux extract of Hibiscus taiwanensis, a 95% ethanol extract of Trillium fortunei and a distilled water extract of gardenia according to a mass ratio of 4: 3, and the concentration of the effective components in the DMSO is 4-8 mg/mL. The invention has the effects of sun screening and anti-photoaging, can be used in cosmetics to achieve the effects of sun screening and anti-aging, and is particularly suitable for rough skin, wrinkle increase, sallow complexion and the like caused by sunlight irradiation; the invention is purely natural, has no stimulation, safety and no toxic and side effects.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a sun-screening and anti-photoaging traditional Chinese medicine composition and application thereof.
Background
Skin aging is a complex biological process, which is divided into endogenous aging and exogenous aging. Endogenous aging is skin aging that occurs over time, and there is gene dependence. Extrinsic aging is caused by external environmental factors, in which UV radiation is the main factor and accounts for 80% of the external influence, and is also called photoaging.
The most prominent clinical features of photoaging are dry skin, loose nodules, increased fine lines, decreased elasticity, and mottled pigmentation. Histologically, photoaged skin has increased melanocytes, hyperpigmentation, a marked reduction in langerhans cells, thickening of the dermis, a marked reduction in collagen fibers, but a substantial increase in elastic fibers. Photoaging at the cellular level is characterized primarily by an excessive accumulation of Reactive Oxygen Species (ROS) and by alterations in the extracellular matrix components.
It has been found that 80% to 90% of skin aging is caused by extrinsic factors, with UV radiation being the most prominent cause of accelerated skin aging. Skin photoaging involves multiple signal molecules and multiple signal transduction pathways, and its molecular mechanisms mainly include DNA damage, oxidative stress, inflammatory response, collagen structural changes and apoptosis, and UV-induced photoaging mechanism is seen in open scientific (resource service) identification code (OSID). The traditional Chinese medicine contains rich protein and various amino acids, lipids, polysaccharides, flavonoids, vitamins, organic acids, alkaloids, saponins and other nutrient substances, has the action mechanism of repairing DNA damage, resisting oxidation and inflammation, repairing skin barrier function, resisting apoptosis and the like, and plays an important role in preventing and delaying skin photoaging.
Disclosure of Invention
The invention aims to provide a sun-screening and anti-photoaging traditional Chinese medicine composition.
The invention also aims to provide application of the sun-screening and anti-photoaging traditional Chinese medicine composition.
The technical scheme of the invention is as follows:
a Chinese medicinal composition with sunscreen and anti-photoaging effects comprises effective components and DMSO, wherein the effective components comprise methanol reflux extract of Hibiscus taiwanensis, 95% ethanol extract of Trillium japonicum and distilled water extract of Gardebuae fructus at a mass ratio of 4: 3, and the concentration of the effective components in DMSO is 4-8 mg/mL.
In a preferred embodiment of the present invention, the preparation method of the methanol reflux extract of hibiscus taiwanensis comprises: grinding the dried rhizomes of the Taiwan mountain hibiscus into powder, performing reflux extraction by using methanol, performing reduced pressure concentration on the obtained product, combining extracts to obtain dark brown syrup, and concentrating the dark brown syrup to obtain paste, namely the methanol reflux extract of the Taiwan mountain hibiscus.
In a preferred embodiment of the present invention, the method for preparing the 95% ethanol extract of Trillium foenum-graecum comprises: drying the roots of the Trillium wilfordii in a constant-temperature oven, crushing the roots by using a traditional Chinese medicine crusher, sieving the crushed roots by using a 60-mesh sieve, fully soaking the roots in 95% ethanol, extracting the roots by using a multi-purpose forced percolation tank repeatedly, concentrating an extracting solution under reduced pressure, and freeze-drying the extracting solution to obtain the 95% ethanol extract of the Trillium wilfordii.
In a preferred embodiment of the present invention, the method for preparing the distilled water extract of gardenia comprises: accurately weighing dried gardenia fruit powder, sieving with a 60-mesh sieve, dissolving the sieved powder in distilled water, stirring, condensing and evaporating, collecting liquid, and filtering to obtain a first filtrate; centrifuging the first filtrate repeatedly to collect supernatant; carrying out suction filtration on the supernatant and reserving a second filtrate, and carrying out rotary evaporation on the second filtrate at 65-75 ℃ to obtain viscous liquid; freeze drying the viscous liquid to obtain dry powder, namely the distilled water extract of cape jasmine.
The application of the sun-screening and anti-photoaging traditional Chinese medicine composition in preparing cosmetics.
In a preferred embodiment of the invention, the content of the sunscreen anti-photoaging traditional Chinese medicine composition in the cosmetic is 0.05-10 wt%.
Further preferably, the content of the sun-screening and anti-photoaging traditional Chinese medicine composition in the cosmetic is 0.1-10 wt%.
A cosmetic contains the above Chinese medicinal composition with sunscreen and anti-photoaging effects.
In a preferred embodiment of the invention, the content of the sunscreen anti-photoaging traditional Chinese medicine composition is 0.05-10 wt%.
Further preferably, the content of the sun-screening and anti-photoaging traditional Chinese medicine composition is 0.1-10 wt%.
The beneficial effects of the invention are:
1. the invention has the effects of sun protection and anti-photoaging, can be used in cosmetics to achieve the effects of sun protection and anti-aging, and is particularly suitable for rough skin, wrinkle increase, sallow complexion and the like caused by sunlight irradiation.
2. The invention is purely natural, has no stimulation, safety and no toxic and side effects.
Drawings
FIG. 1 is a graph showing the results of testing the DPPH radical scavenging ability of the sunscreen anti-photoaging Chinese medicinal composition of embodiment 5 of the present invention.
FIG. 2 is a graph showing the results of detecting HaCaT cytotoxicity of the sunscreen anti-photoaging Chinese medicinal composition in example 5 of the present invention.
Fig. 3 is a diagram showing the result of detection of activity of the sunscreen anti-photoaging Chinese medicinal composition of the present invention in repairing UVB-induced HaCaT cell damage.
FIG. 4 is a graph showing the effect of the sunscreen anti-photoaging Chinese medicinal composition of embodiment 5 on ROS content in photoaged HaCaT cells.
FIG. 5 is a graph showing the effect of the sunscreen photoaging-resistant Chinese medicinal composition of example 5 on the mRNA levels of type I collagen, MMP1 and TIMP1 in HaCaT cells.
FIG. 6 is a graph showing the effect of the sunscreen anti-photoaging Chinese medicinal composition of embodiment 5 on the haemolysis rate of hemoglobin in erythrocytes at different concentrations.
Detailed Description
The technical solution of the present invention will be further illustrated and described below with reference to the accompanying drawings by means of specific embodiments.
Example 1 preparation of methanol reflux extract of Hibiscus taiwanensis
Air-dried rhizome of Hibiscus taiwanensis (20kg) was ground into powder and extracted with methanol under reflux for 6 times. The combined extracts were concentrated under reduced pressure to give a dark brown syrup (850g) which, after concentration, gave a paste (400 g).
Example 2 preparation of 95% ethanol extract of Trillium fortunei
Drying Trillium wilfordii root tuber (6.4kg) in a constant temperature oven at 48 deg.C, pulverizing with a traditional Chinese medicine pulverizer, sieving with a 60 mesh sieve, soaking in 95% ethanol for several hours, extracting with a multi-purpose forced percolation tank, repeating for several times, concentrating the extractive solution under reduced pressure, and freeze drying to obtain Trillium wilfordii ethanol extract (2.4 kg).
Example 3 preparation of distilled Water extract of Gardenia jasminoides Ellis
Accurately weighing 200g of dried gardenia fruit powder, sieving by using a 0.6mm sieve, weighing 5g of sieved powder, dissolving in 250mL of distilled water, stirring for 10min, condensing and evaporating for 2h, collecting liquid and filtering. Centrifuging the filtrate at 3000r/min for 10min, collecting supernatant, and centrifuging for 2 times; and carrying out suction filtration on the supernatant, reserving filtrate, evaporating the filtrate in a rotary evaporator at the temperature of 70 ℃ for 30min to obtain viscous liquid, putting the viscous liquid into a glass plate, pre-freezing the viscous liquid overnight in a refrigerator at the temperature of-20 ℃, then carrying out freeze drying for 24h, and grinding the viscous liquid into powder in a mortar to obtain dry powder (2 g).
EXAMPLE 4 preparation of Sun-protecting anti-photoaging Chinese medicinal composition
Taking the methanol reflux extract of the mountain cotton rose prepared in the examples 1, 2 and 3, the 95% ethanol extract of the trillium and the distilled water of the gardenia prepared in the examples 3 to extract and mix evenly according to the mass ratio of 4: 3 to form effective components, adding DMSO to fully dissolve the effective components until the final concentration of the effective components is 5mg/mL, and obtaining the sun-proof and anti-photoaging traditional Chinese medicine composition.
Example 5 Effect experiment of sunscreen anti-photoaging Chinese medicinal composition
Study of the ability of A to scavenge DPPH free radicals
The activity of the polypeptide for scavenging free radicals was tested using a DPPH free radical scavenging ability test kit (Biyuntian, BC 4750). Thawing 500 μ L of the reagent solution II on ice, adding 2625 μ L of the reagent solution I (absolute ethanol), mixing well, and placing on ice for use. Adding the extracting solution provided by the ImL kit into a brown glass tube of the third reagent, fully and uniformly mixing, subpackaging and storing 50 mu L of each tube, and placing at-20 ℃; taking out the sunscreen anti-photoaging Chinese medicinal composition stock solution from a refrigerator at-20 deg.C, adding DMSO for dilution, and placing on ice for use while preparing solvent for use. Preheating the spectrophotometer for more than 30min, adjusting the wavelength to 515nm, and adjusting the absolute ethyl alcohol to zero. Preparing 10mg/mL vitamin C solution (reagent III) into vitamin C solution larger than 0.3mg/mL with the extract for later use (adding 1.2mL extract into a storage centrifuge tube, and mixing well). Adding the sunscreen anti-photoaging Chinese medicinal composition solution, DMSO, extractive solution and working solution into 1.5ml of LEP tube respectively to make the final concentration of sunscreen anti-photoaging Chinese medicinal composition 62.5 μ M, 125 μ M, 250 μ M, 500 μ M, 1000 μ M. Mixing, standing at room temperature in dark place for 30min, and keeping absorbance at 515 nm. And the absorbance values of the blank tube, the positive control tube, the control tube and the measuring tube are respectively marked as A blank, A positive control, A control and A measurement. The blank tube is only measured 1-2 times. Starting the instrument for preheating and debugging parameters. The sun-screening and anti-photoaging traditional Chinese medicine composition has strong free radical scavenging activity and concentration dependence, and is detected by using an instrument, detection data are recorded, and the result is analyzed and shown in figure 1.
B CCK-8 method for detecting cytotoxicity of sun-screening and anti-photoaging traditional Chinese medicine composition on HaCaT
HaCaT cells were plated in 96-well plates (approximately 4X 10) 4 And/well), after 24 hours, removing the culture medium, adding 100 mu L of serum-free culture medium containing sunscreen and photoaging-resistant traditional Chinese medicine compositions with different concentrations, after 24 hours, adding 10 mu L of CCK-8 reagent into a 96-well plate, and after 2 hours, detecting the OD value at the wavelength of 450 nm. As shown in figure 2, the sunscreen anti-photoaging traditional Chinese medicine composition has no toxicity to HaCaT cells when the concentration is 0-40 mug/mL, and when the concentration is 0-40 mug/mLWhen the concentration reached 80. mu.g/mL, weak cytotoxicity was exhibited.
Detection of activity of C sunscreen anti-photoaging traditional Chinese medicine composition on repair of UVB induced HaCaT cell damage
HaCaT cells were plated in 96-well plates (approximately 4X 10) 4 And/hole), after 24 hours of complete cell adherence, removing the culture medium, adding 100 mu L of serum-free culture medium containing the sunscreen anti-photoaging traditional Chinese medicine composition with different concentrations for pretreatment for 24 hours, then washing for 3 times by using PBS, and irradiating the cells by using a UVB ultraviolet irradiation instrument to HaCaT cells with a certain irradiation dose. After HaCaT cell irradiation, 1% FBS fresh medium was replaced, 24h later, 10. mu.L of CCK-8 reagent was added to the 96-well plate, and 2h later, OD value was measured at a wavelength of 450 nm. The result is shown in fig. 3, and it can be seen that the sunscreen anti-photoaging traditional Chinese medicine composition has a significant protective effect on the photodamage of HaCaT cells when the concentration is 0-40 μ g/mL, and the protective effect is weakened when the concentration is 80 μ g/mL.
Influence of D sun-screening anti-photoaging traditional Chinese medicine composition on ROS content in photoaging HaCaT cells
ROS production in HaCaT cells following UVB irradiation was measured by fluorescence microscopy using DCFH 2-DA. First, HaCaT cells (1.5X 10) 5 Per well) were cultured in medium supplemented with 10% FBS. Then, when 80% cell density was reached, the medium was replaced with fresh one. After pretreatment and UVB irradiation of a certain concentration of the sun-screening and anti-photoaging traditional Chinese medicine composition, cells are washed by PBS, incubated with 10 mu M DCFH2-DA in PBS at 37 ℃ for 30min, and then washed by PBS buffer. And finally, measuring fluorescence under a 485nm excitation and 535nm emission recording spectrophotometer, wherein the result is shown in figure 4, and the sunscreen anti-photoaging traditional Chinese medicine composition can obviously reduce the content of ROS in photoaging HaCaT cells when the concentration is 0-40 mu g/mL.
E real-time fluorescent quantitative PCR
RNA was extracted from HaCaT cells pretreated with 40 μ g/mL sunscreen anti-photoaging Chinese medicinal composition and induced by UVB using RNeasy mini kit (Qiagen, Pudong, Shanghai, China), and 500ng RNA was reverse transcribed per sample using GoScript reverse transcription mix (Promega, WI). EVA Green Supermix (Bio-Rad, CA) was then used for real-time quantification of PCR, beta-actin is used as an internal reference. Primers for MMP1, type I collagen, and TIMP1 were designed using Primer5, as shown in table 1.2 -ΔCt The method is used to quantify relative gene expression.
TABLE 1
The results are shown in fig. 5, 40 μ g/mL of the sunscreen anti-photoaging traditional Chinese medicine composition can partially reverse the mRNA expression reduction of type I collagen and TIMP1 and the mRNA expression up-regulation of MMP1 of HaCaT cells induced by UVB.
Haemolysis test of erythrocytes
Taking 7mL of blood from the heart of a healthy rabbit, adding 1mL of 2% potassium oxalate solution to prepare fresh anticoagulated rabbit blood, adding PBS10mL for dilution, and storing in a refrigerator at 4 ℃ for later use. Preparing a sample series solution. For each sample, 8 test doses were established to ensure adequate mixing of each diluted sample suspension. Hemolysis and protein denaturation assays: each sample group with 2 concentrations is plugged into a test tube, and 10mL of sample suspension is added into each tube; the negative control group 2 was mounted in tubes, and PBS10mL was added to each tube; the positive control group 2 was mounted on a plug tube, and 10mL of distilled water was added to each tube. 0.2mL of diluted rabbit blood was added to each tube, gently mixed, and incubated at 37 ℃ for 30 min. After the completion of the centrifugation, the supernatant was centrifuged for 10min at 2000rmin and transferred to a cuvette, and the absorbance (A) was measured at 540nm, 560nm and 575nm in a spectrophotometer. The hemolysis rate (%) is calculated according to the formula (I), and the hemoglobin denaturation index (D I% >) is calculated according to the formula (II). Centrifugation supernatant A in which R1 is blank control distilled water 575 /A 540 A value; r2 is sample centrifuge supernatant A 575 /A 540 A value; SDS centrifugation supernatant A with R3 as positive control 575 /A 540 The value is obtained. The main observation is the concentration of the sample at which 50% of the erythrocytes are Hemolyzed (HD) 50 ) Protein Denaturation Index (DI), HD 50 Ratio to denaturation index DI (IP ═ HD) 50 /D I)。
The results are shown in FIG. 6, where the concentration of the sunscreen anti-photoaging Chinese medicinal composition is below 100 μ g/mL, and the composition is insoluble in blood.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, which is defined by the appended claims.
Claims (10)
1. A sun-screening and anti-photoaging traditional Chinese medicine composition is characterized in that: the composition comprises effective components and DMSO, wherein the effective components comprise methanol reflux extract of Hibiscus taiwanensis, 95% ethanol extract of Trillium japonicum and distilled water extract of Gardenia jasminoides Ellis at a mass ratio of 4: 3, and the concentration of the effective components in DMSO is 4-8 mg/mL.
2. The sun-screening anti-photoaging traditional Chinese medicine composition of claim 1, wherein: the preparation method of the methanol reflux extract of the mountain Taiwan hibiscus comprises the following steps: grinding the dried rhizomes of the Taiwan mountain hibiscus into powder, performing reflux extraction by using methanol, performing reduced pressure concentration on the obtained product, combining extracts to obtain dark brown syrup, and concentrating the dark brown syrup to obtain paste, namely the methanol reflux extract of the Taiwan mountain hibiscus.
3. The sun-screening anti-photoaging traditional Chinese medicine composition of claim 1, wherein: the preparation method of the Trillium fortunei 95% ethanol extract comprises the following steps: drying the roots of the Trillium wilfordii in a constant-temperature oven, crushing the roots by using a traditional Chinese medicine crusher, sieving the crushed roots by using a 60-mesh sieve, fully soaking the roots in 95% ethanol, extracting the roots by using a multi-purpose forced percolation tank repeatedly, concentrating an extracting solution under reduced pressure, and freeze-drying the extracting solution to obtain the 95% ethanol extract of the Trillium wilfordii.
4. The sun-screening anti-photoaging traditional Chinese medicine composition of claim 1, wherein: the preparation method of the distilled water extract of gardenia comprises the following steps: accurately weighing dried gardenia fruit powder, sieving with a 60-mesh sieve, dissolving the sieved powder in distilled water, stirring, condensing and evaporating, collecting liquid, and filtering to obtain a first filtrate; centrifuging the first filtrate repeatedly to collect supernatant; carrying out suction filtration on the supernatant and reserving a second filtrate, and carrying out rotary evaporation on the second filtrate at 65-75 ℃ to obtain viscous liquid; freeze drying the viscous liquid to obtain dry powder, namely the distilled water extract of cape jasmine.
5. Use of the sunscreen anti-photoaging traditional Chinese medicine composition according to any one of claims 1 to 4 in the preparation of cosmetics.
6. The use of claim 5, wherein: the content of the sun-screening and anti-photoaging traditional Chinese medicine composition in the cosmetics is 0.05-10 wt%.
7. The use of claim 6, wherein: the content of the sun-screening and anti-photoaging traditional Chinese medicine composition in the cosmetics is 0.1-10 wt%.
8. A cosmetic characterized by: a sunscreen anti-photoaging Chinese medicinal composition comprising the composition as claimed in any one of claims 1 to 4.
9. The use of claim 8, wherein: the content of the sun-screening and anti-photoaging traditional Chinese medicine composition is 0.05-10 wt%.
10. The use of claim 9, wherein: the content of the sun-screening and anti-photoaging traditional Chinese medicine composition is 0.1-10 wt%.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103393567A (en) * | 2013-07-29 | 2013-11-20 | 嘉文丽（福建）化妆品有限公司 | Anti-acne cosmetic containing Taiwan hibiscus taiwanensis extract |
| CN107949370A (en) * | 2015-09-09 | 2018-04-20 | 皮埃尔法布雷医药公司 | For making the capejasmine extract of pigmenting of skin |
| CN108354859A (en) * | 2018-04-28 | 2018-08-03 | 云南省药物研究所 | A kind of preparation method of the anti-acne chemical product of the extract containing trilliaceae and application |
| CN112076126A (en) * | 2020-09-28 | 2020-12-15 | 嘉文丽（福建）化妆品有限公司 | Separation method of whitening functional components in hibiscus mutabilis and application of whitening functional components in cosmetics |
| CN114588225A (en) * | 2021-12-20 | 2022-06-07 | 嘉文丽（福建）化妆品有限公司 | Method for extracting and converting saponin in Trillium fortunei |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103393567A (en) * | 2013-07-29 | 2013-11-20 | 嘉文丽（福建）化妆品有限公司 | Anti-acne cosmetic containing Taiwan hibiscus taiwanensis extract |
| CN107949370A (en) * | 2015-09-09 | 2018-04-20 | 皮埃尔法布雷医药公司 | For making the capejasmine extract of pigmenting of skin |
| CN108354859A (en) * | 2018-04-28 | 2018-08-03 | 云南省药物研究所 | A kind of preparation method of the anti-acne chemical product of the extract containing trilliaceae and application |
| CN112076126A (en) * | 2020-09-28 | 2020-12-15 | 嘉文丽（福建）化妆品有限公司 | Separation method of whitening functional components in hibiscus mutabilis and application of whitening functional components in cosmetics |
| CN114588225A (en) * | 2021-12-20 | 2022-06-07 | 嘉文丽（福建）化妆品有限公司 | Method for extracting and converting saponin in Trillium fortunei |
Non-Patent Citations (1)
| Title |
|---|
| 国家食品药品监督管理总局: "《化妆品安全技术规范》", pages: 35 * |
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