CN114947733A - Eye tissue imaging device and equipment - Google Patents

Abstract

The invention discloses an eye tissue imaging device and equipment, wherein the eye tissue imaging device comprises: the sighting target unit is used for adjusting the position and the angle of an eyeball; a fundus camera imaging unit for imaging fundus tissues; a white eye imaging unit for imaging a superficial ocular surface; the optical coherence tomography unit is used for imaging the anterior segment and the posterior segment of the eye; and the optical path adjusting unit is used for coupling the light beam emitted by the sighting target unit, the light beam emitted by the eyeground camera imaging unit and the light beam emitted by the optical coherence tomography unit to the eyeball and coupling the light beam scattered by the eyeball to the eyeground camera imaging unit, the white-eye imaging unit and the optical coherence tomography unit respectively so as to acquire image information of different parts of the eyeball simultaneously. Therefore, the image information of different parts of the eyeball can be acquired simultaneously, and the eye diseases can be diagnosed accurately.

Description

Eye tissue imaging device and equipment

Technical Field

The present invention relates to the field of eye tissue imaging technologies, and in particular, to an eye tissue imaging apparatus and an eye tissue imaging device.

Background

The eye is the main organ of the human perception world, and foreign objects are imaged on the retina through the human eye and then transmitted to the brain through the optic nerve. Thus, if the eye tissue becomes diseased, vision will be impaired.

In the related art, an OCT (Optical Coherence Tomography) ophthalmic imaging system based on a fundus camera can image only tissues such as an anterior segment, a posterior segment (including a fundus), and the like of an eye of a subject, and cannot image white eye portions (a bulbar conjunctiva and a sclera) of a superficial eye. The illumination system of the white eye imaging device can only illuminate superficial ocular tissues, the optical path system can only image the superficial ocular tissues, and the illumination system and the optical path system cannot be used for imaging anterior segment tissues and posterior segment tissues (including eyeground). Therefore, both OCT and white eye imaging devices based on fundus cameras cannot simultaneously acquire images of the superficial ocular surface, anterior segment, posterior segment and fundus tissue to accurately diagnose ocular disease.

Disclosure of Invention

The present invention is directed to solving, at least to some extent, one of the technical problems in the related art. Therefore, a first object of the present invention is to provide an eye tissue imaging apparatus, which can simultaneously acquire image information of different parts of an eyeball by coupling a light beam emitted from an optotype unit, a fundus camera imaging unit, and an optical coherence tomography unit and a light beam scattered from the eyeball through an optical path adjusting unit, thereby accurately diagnosing an eye disease.

A second object of the invention is to propose an ophthalmic tissue imaging apparatus.

To achieve the above object, a first aspect of the present invention provides an eye tissue imaging apparatus, including: the sighting target unit is used for adjusting the position and the angle of an eyeball; a fundus camera imaging unit for imaging fundus tissues; a white eye imaging unit for imaging a superficial eye surface; the optical coherence tomography unit is used for imaging the anterior segment and the posterior segment of the eye; and the optical path adjusting unit is used for coupling the light beam emitted by the visual target unit, the light beam emitted by the eyeground camera imaging unit and the light beam emitted by the optical coherence tomography unit to the eyeball and coupling the light beam scattered by the eyeball to the eyeground camera imaging unit, the white eye imaging unit and the optical coherence tomography unit respectively so as to acquire image information of different parts of the eyeball simultaneously.

According to the eye tissue imaging device provided by the embodiment of the invention, the position and the angle of the eyeball are adjusted by the visual target unit to select the imaging area of the eye tissue, the light beam emitted by the visual target unit, the light beam emitted by the eyeground camera imaging unit and the light beam emitted by the optical coherence tomography unit are coupled to the eyeball together by the light path adjusting unit, and the light beam scattered by the eyeball is coupled to the eyeground camera imaging unit, the white eye imaging unit and the optical coherence tomography unit respectively, so that the image information of different parts of the eyeball can be acquired simultaneously, and the eye disease can be diagnosed accurately.

In addition, according to the eye tissue imaging apparatus of the above embodiment of the present invention, the following additional features may be further provided:

according to one embodiment of the invention, a fundus camera imaging unit includes a fundus illuminating light source for providing a first light beam for illuminating fundus tissue, an illumination beam adjustment optical path, a first coupling optical path, a first imaging optical path, and a fundus imaging camera; the illumination light beam adjusting light path is used for adjusting the first light beam to form a surface illumination light beam; a first coupling optical path for reflecting the surface illumination beam to the optical path adjusting unit to be coupled to the fundus tissue through the optical path adjusting unit; an optical path adjusting unit for coupling the light beam emitted from the fundus tissue to the first imaging optical path; and the first imaging optical path is used for processing the light beam scattered by the fundus tissue coupled by the optical path adjusting unit and sending the processed light beam scattered by the fundus tissue into the fundus imaging camera for imaging.

According to one embodiment of the invention, the white-eye imaging unit comprises a white-eye illumination light source, an illumination light path, a second coupling light path, a second imaging light path and a white-eye imaging camera, wherein the white-eye illumination light source is adapted to provide a second light beam for illuminating a superficial eye surface; an illumination optical path for delivering the second light beam to the superficial eye; the light path adjusting unit is also used for coupling the light beam scattered by the superficial eye surface to a second coupling light path; the second coupling optical path is used for coupling the light beam scattered by the superficial eye surface coupled by the optical path adjusting unit to the second imaging optical path for processing;

and the second imaging optical path is used for sending the light beam scattered by the treated superficial eye surface into the white eye imaging camera for imaging.

According to an embodiment of the present invention, the second coupling optical path is further configured to couple the light beam emitted from the target unit to the optical path adjusting unit, so as to couple the light beam emitted from the target unit to the eyeball through the optical path adjusting unit.

According to one embodiment of the invention, the optical coherence tomography unit comprises a swept-frequency light source, a first light splitting and interference optical path, a first reference optical path, a first sample optical path and a photodetector, wherein the swept-frequency light source is used for providing a third light beam for illuminating the anterior segment and the posterior segment of the eye; the first light splitting and interference optical path is used for performing light splitting processing on the third light beam so as to provide a first light splitting beam for the first reference optical path and a second light splitting beam for the first sample optical path; the first reference light path is used for reflecting the first light splitting beam and transmitting the first light splitting beam subjected to reflection processing to the first light splitting and interference light path; the first sample optical path is used for processing the second split light beam and transmitting the processed second split light beam to the optical path adjusting unit so as to couple the processed second split light beam to the anterior segment and the posterior segment of the eye through the optical path adjusting unit; the optical path adjusting unit is also used for coupling the light beams scattered by the anterior segment and the posterior segment of the eye to a first sample optical path, processing the light beams scattered by the anterior segment and the posterior segment of the eye through the first sample optical path and transmitting the processed light beams to a first light splitting and interference optical path; the first light splitting and interference light path is also used for interfering the first light splitting light beam subjected to reflection processing by the first reference light path with light beams scattered from the anterior segment and the posterior segment of the eye of the first sample light path and transmitting the interfered light beams to the photoelectric detector; and the photoelectric detector is used for converting the interfered light beam into an electric signal and carrying out subsequent spectral analysis.

According to one embodiment of the invention, an optical coherence layer imaging unit comprises a continuous broad spectrum light source, a second split and interference optical path, a second reference optical path, a second sample optical path, a grating, a converging lens, a camera; a broad spectrum light source for providing a fourth light beam for illuminating the anterior and posterior segments of the eye;

the second light splitting and interference light path is used for splitting the fourth light beam so as to provide a third light splitting light beam for the second reference light path and a fourth light splitting light beam for the second sample light path; the second reference light path is used for reflecting the third split light beam and transmitting the reflected third split light beam to the second light splitting and interference light path; the second sample light path is used for processing the fourth light beam and transmitting the processed fourth light beam to the light path adjusting unit so as to couple the processed fourth light beam to the anterior segment and the posterior segment of the eye through the light path adjusting unit; the optical path adjusting unit is also used for coupling the light beams scattered by the anterior segment and the posterior segment of the eye to a second sample optical path, and the light beams scattered by the anterior segment and the posterior segment of the eye are processed by the second sample optical path and then transmitted to a second light splitting and interference optical path; and the second light splitting and interference light path is also used for interfering the third light splitting beam subjected to reflection processing by the second reference light path with the beams scattered from the anterior segment and the posterior segment of the eye of the second sample light path, and transmitting the interfered beams to the camera for spectral analysis.

According to one embodiment of the present invention, the optical path adjusting unit includes a first dichroic mirror and a second dichroic mirror.

According to one embodiment of the invention, the first coupling light path comprises a hollow mirror.

According to one embodiment of the invention, the second coupled optical path comprises a beam splitter.

To achieve the above object, an embodiment of a second aspect of the present invention proposes an eye tissue imaging apparatus including the eye tissue imaging device described in the above embodiment.

According to the eye tissue imaging device provided by the embodiment of the invention, by implementing the eye tissue imaging device described in the above embodiment, the image information of different parts of the eyeball can be acquired simultaneously, so that the eye disease can be diagnosed accurately.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

FIG. 1 is a block schematic diagram of an eye tissue imaging apparatus according to one embodiment of the invention;

FIG. 2 is a schematic diagram of an eye tissue imaging apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a structure of an imaging unit of an optically coherent layer in accordance with one embodiment of the present invention;

FIG. 4 is a schematic structural diagram of an eye tissue imaging apparatus according to another embodiment of the present invention;

FIG. 5 is a schematic diagram of a structure of an imaging unit of an optically coherent layer in accordance with another embodiment of the present invention;

FIG. 6 is a schematic diagram of an eye tissue imaging apparatus according to an embodiment of the invention;

FIG. 7 is a block schematic diagram of an eye tissue imaging apparatus according to one embodiment of the invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to explain the present invention and should not be construed as limiting the present invention.

The present application was made based on the recognition and study of the following problems by the inventors:

conventional imaging of eye tissue mainly includes fundus camera imaging (ophthalmoscope), optical coherence tomography and white eye imaging (bulbar conjunctiva and sclera imaging). The white eye imaging can analyze information such as the color, blood vessels and the like of the bulbar conjunctiva and sclera of the anterior segment of the eye, so that the pathological condition of the eye can be known, and the health condition of a human body can be predicted. The fundus camera imaging can illuminate the fundus by white light or near infrared light, and images fundus images on the camera, or can inject fluorescein or dye into blood vessels, and excite the fluorescein or dye by excitation light with specific wavelength to image the blood vessels of the fundus. The optical coherence tomography is a label-free high-resolution three-dimensional medical imaging technology, adopts visible light or near infrared light, utilizes the interference principle of light to scan and image eye tissues, and has the label-free three-dimensional high-resolution characteristic suitable for imaging anterior and posterior eye segments.

From the aspect of image characteristics, white eye imaging and fundus camera imaging can obtain two-dimensional image information, and optical coherence tomography can realize three-dimensional imaging of eye tissues.

In the detection process, the white-eye imaging and the optical coherence tomography do not need to stain or mark eye tissues or blood vessels; fundus camera imaging can be either marked with fluorescein or dye or unmarked.

From the imaging part, the fundus camera imaging mainly images the fundus tissues (such as retina) of the posterior segment of the eye, the white eye imaging mainly images the bulbar conjunctiva and sclera of the anterior segment of the eye, and the optical coherence tomography imaging can image both the anterior segment of the eye and the posterior segment of the eye.

In the related art, neither optical coherence tomography based on fundus cameras nor white eye imaging devices can simultaneously acquire images of superficial ocular surfaces, anterior ocular segment, posterior ocular segment, and fundus tissues. Therefore, the present invention provides an ocular tissue imaging device capable of simultaneously acquiring images of superficial ocular tissues, anterior ocular segments, posterior ocular segments, and fundus tissues, thereby predicting the health condition of a human body and accurately diagnosing ocular diseases.

FIG. 1 is a block schematic diagram of an eye tissue imaging apparatus according to one embodiment of the invention. As shown in fig. 1, the eye tissue imaging apparatus includes a sighting target unit 100, a fundus camera imaging unit 200, a white eye imaging unit 300, an optical coherence tomography unit 400, and an optical path adjusting unit 500. The sighting target unit 100 is used for adjusting the position and the angle of an eyeball; a fundus camera imaging unit 200 for imaging fundus tissues; a white eye imaging unit 300 for imaging a superficial eye surface; an optical coherence tomography unit 400 for imaging the anterior segment and the posterior segment of the eye; an optical path adjusting unit 500 for coupling the light beam emitted from the sighting target unit 100, the light beam emitted from the fundus camera imaging unit 200, and the light beam emitted from the optical coherence tomography unit 400 to the eyeball together, and coupling the light beam scattered by the eyeball to the fundus camera imaging unit 200, the white eye imaging unit 300, and the optical coherence tomography unit 400, respectively, so as to simultaneously acquire image information of different portions of the eyeball.

Specifically, the sighting target unit 100 includes a plurality of positions of bright spots which can be extinguished controllably, and the eyeballs can focus on the bright spots through a lens group (not shown) and the light path adjusting unit 500, so that the positions and angles of the eyeballs can be adjusted, and each imaging unit can conveniently image different positions of the eyeballs; the illumination beam of the fundus camera imaging unit 200 illuminates the fundus tissue (e.g., retina) through the optical path adjusting unit 500, and the fundus tissue generates reflected light and scattered light, which reach the fundus camera imaging unit 200 again through the optical path adjusting unit 500 to image the fundus tissue; the illumination light beam of the white eye imaging unit 300 illuminates superficial ocular tissues (e.g., the bulbar conjunctiva and sclera) which generate reflected light and scattered light which pass through the optical path adjusting unit 500 to the white eye imaging unit 300 to image the superficial ocular tissues; the illumination beam of the optical coherence tomography unit 400 illuminates the anterior segment (e.g., cornea, iris, ciliary body, crystalline lens) and the posterior segment (e.g., vitreous body, retina, choroid, etc.) of the eye through the optical path adjusting unit 500, and the anterior segment and the posterior segment generate reflected light and scattered light, which reach the optical coherence tomography unit 400 through the optical path adjusting unit 500 again to image the anterior segment and the posterior segment of the eye.

In some embodiments of the present invention, as shown in FIG. 2, a fundus camera imaging unit 200 includes a fundus illuminating light source 220, an illumination beam adjustment optical path 240, a first coupling optical path 260, a first imaging optical path 280, and a fundus imaging camera 290, wherein the fundus illuminating light source 220 provides a first light beam for illuminating fundus tissue; an illumination beam adjustment optical path 240 for adjusting the first beam to form a surface illumination beam; a first coupling optical path 260 for reflecting the surface illumination light beam to the optical path adjusting unit 500 to be coupled to the fundus tissue through the optical path adjusting unit 500; an optical path adjusting unit 500 for coupling the light beam scattered from the fundus tissue to the first imaging optical path 280; the first imaging optical path 280 is used for processing the light beam emitted from the fundus tissue coupled by the optical path adjusting unit 500 and sending the light beam scattered by the processed fundus tissue to the fundus imaging camera 290 for imaging.

Specifically, the fundus illuminating light source 220 (e.g., white light, near infrared light) emits a first light beam, which is adjusted by the illuminating light beam adjusting optical path 240 to form a surface illuminating light beam, so as to uniformly illuminate the fundus tissue, the surface illuminating light beam is reflected by the first coupling optical path 260, enters the optical path adjusting unit 500, is transmitted by the optical path adjusting unit 500, enters the lens group 600 in front of the eyeball, and reaches the fundus tissue, the fundus tissue generates reflected light and scattered light (light beam scattered by the eyeball), the reflected light and the scattered light pass through the optical path adjusting unit 500, enter the first coupling optical path 260, pass through the transmission of the first coupling optical path 260, enter the first imaging optical path 280, and pass through the optical processing (e.g., focusing and light intensity adjustment) of the first imaging optical path 280 to enter the fundus imaging camera 290 for imaging. The illumination beam adjusting optical path 240 may include a diffuse scattering sheet (diffuse scattering processing) and an optical filter (stray light is eliminated, and imaging quality is improved); the first imaging light path 280 may include a lens (focusing the light beam), a filter (eliminating stray light, improving imaging quality), and a grating (adjusting the light intensity). The fundus imaging camera 290 may perform surface imaging using a CCD (Charge coupled device) camera or a CMOS (Complementary Metal Oxide Semiconductor) camera, may perform line scanning imaging using a line scanning and line detecting method, or may perform point scanning imaging using a point scanning and point detecting method. Note that an electric detecting device (for example, PMT (Photomultiplier tube)) may be used instead of the fundus imaging camera 290.

It should be noted that the first coupling optical path 260 may be a hollow mirror. Specifically, the surface illumination light beam enters the optical path adjusting unit 500 by refraction of the hollow mirror, and the reflected light and scattered light generated from the fundus tissue pass through the optical path adjusting unit 500, are transmitted through the center hole of the hollow mirror, and enter the first imaging optical path 280.

In some embodiments of the present invention, as shown in fig. 2, the white eye imaging unit 300 comprises a white eye illumination source 320, an illumination optical path 340, a second coupled optical path 360, a second imaging optical path 380, and a white eye imaging camera 390. Wherein the white eye illumination source 320 is for providing a second light beam for illuminating the superficial eye; an illumination optical path 340 for delivering the second light beam to the superficial eye; the light path adjusting unit 500 is further configured to couple the light beam scattered by the superficial eye to the second coupling light path 360; the second coupling optical path 360 is used for coupling the light beam scattered by the superficial eye coupled by the optical path adjusting unit 500 to the second imaging optical path 380 for processing; and a second imaging optical path 380 for sending the processed light beam scattered by the superficial eye to the white eye imaging camera 390 for imaging.

Specifically, the white-eye illumination light source 320 (e.g., single or multi-directional LED point light source, single or multi-directional line light source, annular LED light source) emits a second light beam, which is adjusted (e.g., lens) by the illumination light path 340, and the second light beam is laterally irradiated to the superficial eye surface, which generates reflected light and scattered light (light beam scattered by the eyeball), and the reflected light and the scattered light pass through the lens set 600, enter the light path adjusting unit 500, pass through the transmission and reflection of the light path adjusting unit 500, enter the second coupling light path 360, pass through the reflection of the second coupling light path 360, enter the second imaging light path 380 (e.g., lens), pass through the focusing of the second imaging light path 380, and enter the white-eye imaging camera 390 for imaging. The white eye imaging camera 390 may be a CCD camera, a general camera, a camera of a mobile terminal, or the like.

In this example, the white-eye imaging can analyze the color, blood vessel, etc. of the bulbar conjunctiva and sclera of the anterior segment of the eye, and can not only understand the pathological condition of the eye, but also predict the health condition of the human body.

In some embodiments of the present invention, the second coupling optical path 360 is further configured to couple the light beam emitted from the target unit 100 to the optical path adjusting unit 500, so as to couple the light beam emitted from the target unit 100 to the eyeball through the optical path adjusting unit 500.

Specifically, as shown in fig. 2, the target unit 100 corresponds to the second coupling optical path 360, and the light beam (emitted from the light source at the bright spot) emitted from the target unit 100 enters the optical path adjusting unit 500 through the transmission of the second coupling optical path 360, and is reflected and transmitted by the optical path adjusting unit 500 to enter the lens group 600 in front of the eyeball, so that the eyeball can observe the target, and the fundus camera can just clearly image.

It should be noted that the second coupling optical path 360 includes a beam splitter. Specifically, the light beam emitted from the sighting target unit 100 enters the optical path adjusting unit 500 through the transmission of the beam splitter, and the reflected light and the scattered light scattered by the superficial eye surface reach the beam splitter after being adjusted by the optical path adjusting unit 500, and enter the second imaging optical path 380 through the refraction of the beam splitter.

In some embodiments of the present invention, the optical coherence tomography unit 400 includes the swept frequency light source 410, the first split and interference optical path 430, the first reference optical path 470, the first sample optical path 450, and the photodetector 471. Wherein, the swept-frequency light source 410 is configured to provide a third light beam for illuminating the anterior segment and the posterior segment of the eye; a first split and interference optical path 430 for splitting the third beam to provide a first split beam to the first reference optical path 470 and a second split beam to the first sample optical path 450; a first reference optical path 470, configured to perform reflection processing on the first split optical beam, and transmit the reflected first split optical beam to the first splitting and interference optical path 430; a first sample optical path 450, configured to process the second split optical beam, and transmit the processed second split optical beam to the optical path adjusting unit 500, so as to couple the processed second split optical beam to the anterior segment and the posterior segment through the optical path adjusting unit 500; the optical path adjusting unit 500 is further configured to couple the light beams scattered by the anterior segment and the posterior segment to the first sample optical path 450, and transmit the light beams scattered by the anterior segment and the posterior segment to the first light splitting and interference optical path 430 after being processed by the first sample optical path 450; the first light splitting and interference optical path 430 is further configured to interfere the first split light beam reflected by the first reference optical path 470 with the light beams scattered from the anterior segment and the posterior segment of the eye of the first sample optical path 450, and transmit the interfered light beam to the photodetector 471; and a photodetector 471 for converting the interfered light beam into an electrical signal.

Specifically, as shown in fig. 2 and 3, the swept optical source 410 may output a single-wavelength (or narrow-band) laser (third beam) at a unit time, the first light splitting and interference optical path 430 may include a fiber coupler 431 and a fiber coupler 432, the first reference optical path 470 may include a fiber circulator 472, a fiber collimator 474 and a mirror 476, and the first sample optical path 450 includes a fiber circulator 452, a fiber collimator mirror 454 and a galvanometer mirror 458.

In practical application, the third light beam emitted by the swept-frequency light source 410 enters the fiber coupler 431, is split by the fiber coupler 431 to obtain a first split light beam and a second split light beam, the first split light beam enters the fiber circulator 472 from the port a1, then enters the fiber collimator 474 from the port B1 of the fiber circulator 472 to convert the first split light beam into collimated light 1, the collimated light 1 is reflected by the mirror 476 and returns to the fiber collimator 474, and then returns to the fiber circulator 472 through the port B1 and enters the fiber coupler 432 from the port C1; the second split light beam enters the fiber circulator 452 from the port a2, and then enters the fiber collimator 454 from the port B2 of the fiber circulator 452, the second split light beam is converted into collimated light 2, the collimated light 2 is adjusted by the vibrating mirror 458, the collimated light 2 enters the light path adjusting unit 500, and enters the lens group 600 after being adjusted by the light path adjusting unit 500 to illuminate the anterior segment and the posterior segment of the eye, at this time, the anterior segment and the posterior segment of the eye generate reflected light and scattered light, the reflected light and the scattered light are reflected by the light path adjusting unit 500 to enter the vibrating mirror 458 of the first sample light path 450, and after being adjusted by the vibrating mirror 458, the adjusted reflected light and scattered light enter the fiber collimator 454, after being adjusted by the fiber collimator 454, enter the fiber circulator 452 from the port B2, and enter the fiber coupler 432 through the port C2. The light beam of the first reference optical path 470 (the light beam passing through the port C1) and the light beam of the first sample optical path 450 (the light beam passing through the port C2) interfere in the fiber coupler 432 to form interference light 1, and the interference light 1 is detected by the photodetector 471, subjected to data acquisition and processing by the signal acquisition card 475, and then input to the computer 473 for display.

It should be noted that the first reference optical path may further include a fiber circulator, an optical delay line, and a faraday rotator mirror, and the first reference optical path may be connected to the fiber coupler 431 and the fiber coupler 432 through optical fibers.

Alternatively, in other embodiments of the present invention, as shown in fig. 4, the optical coherence layer imaging unit includes a continuous wide spectrum light source 420, a second split and interference optical path 440, a second reference optical path 480, a second sample optical path 460, a grating 481, a converging lens 482, a camera 483; a continuous broad spectrum light source 420 for providing a fourth light beam that illuminates the anterior and posterior segments of the eye; a second splitting and interference optical path 440, configured to split the fourth light beam to provide a third split light beam to the second reference optical path 480 and a fourth split light beam to the second sample optical path 460; a second reference optical path 480, configured to perform reflection processing on the third split optical beam, and transmit the reflected third split optical beam to the second light splitting and interference optical path 440; a second sample optical path 460, configured to process the fourth split light beam, and transmit the processed fourth split light beam to the optical path adjusting unit 500, so as to couple the processed fourth split light beam to the anterior segment and the posterior segment through the optical path adjusting unit 500; the optical path adjusting unit 500 is further configured to couple the light beams scattered by the anterior segment and the posterior segment to the second sample optical path 460, and transmit the light beams scattered by the anterior segment and the posterior segment to the second light splitting and interference optical path 440 after processing the light beams through the second sample optical path 460; the second light splitting and interference optical path 440 is further configured to interfere the third split light beam reflected by the second reference optical path 480 with the light beams scattered from the anterior segment and the posterior segment of the eye in the second sample optical path 460, and transmit the interfered light beams to the camera for spectral analysis.

Specifically, as shown in fig. 4 and 5, the second split and interference optical path 440 may include an optical isolator 444 and a fiber coupler 446, the second reference optical path 480 may include a fiber collimator 486 and a mirror 488, and the second sample optical path 460 includes a fiber collimator 462 and a galvanometer 464.

In practical applications, the fourth light beam emitted from the continuous broad spectrum light source 420 enters the optical isolator 444, and enters the fiber coupler 446 after being isolated, so as to obtain a third split light beam and a fourth split light beam, wherein the third split light beam enters the fiber collimator 486 to convert the third split light beam into collimated light 3, the collimated light 3 is reflected by the reflector 488 and returns to the fiber collimator 486, and enters the fiber coupler 446 again, the fourth split light beam enters the fiber collimator 462 to convert the fourth split light beam into collimated light 4, the collimated light 4 is adjusted by the vibrating mirror 464, the collimated light 4 enters the light path adjusting unit 500, and enters the lens group 600 after being adjusted by the light path adjusting unit 500 to illuminate the anterior segment and the posterior segment, and then the anterior segment and the posterior segment generate reflected light and scattered light, and the reflected light and the scattered light pass through the lens group 600 and the light path adjusting unit 500, and enters the vibrating mirror 464 of the second sample optical path 460 after being reflected by the optical path adjusting unit 500, and the adjusted reflected light and scattered light enter the fiber collimator 462 after being adjusted by the vibrating mirror 464 and enter the fiber coupler 446 after being adjusted by the fiber collimator 462. The light beam of the second reference optical path 480 (collimated light 3 returning to the fiber collimator 486 again) and the light beam of the second sample optical path 460 (reflected light and scattered light passing through the fiber collimator 454) interfere in the fiber coupler 446 to form interference light 2. Because the interference light 2 contains interference light beams with different wavelengths, the interference light beams with different wavelengths can be spatially separated through the grating 481, and are projected to different positions of the camera 483 after passing through the converging lens 482, so that simultaneous detection of the intensities of the interference light with different wavelengths is realized. The light signal detected by the camera 483 is input to the computer 473 and displayed. The optical isolator 444 is based on the characteristic of unidirectional propagation and is used for isolating the continuous broad spectrum light source 420 and the optical fiber coupler 446, so that the fourth light beam can only enter the optical fiber coupler 446 from the continuous broad spectrum light source 420, but cannot reversely propagate back to the continuous broad spectrum light source 420, and the continuous broad spectrum light source 420 is prevented from being damaged.

It should be noted that, a one-dimensional signal along the depth direction Z of the fundus tissue can be obtained by acquiring interference light (including interference light 1 and interference light 2), and the position of the eyeball irradiated can be changed by combining with a galvanometer, so that two-dimensional scanning in the X-Y direction can be realized, and three-dimensional imaging of the eyeball can be realized.

In some embodiments of the present invention, as illustrated in fig. 2 and 4, the optical path adjusting unit 500 includes a first dichroic mirror 520 and a second dichroic mirror 540.

Specifically, the first dichroic mirror 520 is used to transmit the light beam emitted from the fundus camera imaging unit 200 and the light beam emitted from the optotype unit 100, and the second dichroic mirror 540 is used to transmit the light beam emitted from the fundus camera imaging unit 200 and the light beam emitted from the reflection sample optical path. Also, the first dichroic mirror 520 is also used to transmit the light beam scattered by the eyeballs to the fundus camera imaging unit 200 and to reflect to the white eye imaging unit 300, and the second dichroic mirror 540 is also used to transmit the light beam scattered by the eyeballs to the first dichroic mirror 520 and to reflect to the optical coherence tomography unit 400.

In summary, according to the eye tissue imaging apparatus of the embodiment of the invention, the position and the angle of the eye ball are adjusted by the visual target unit to select the imaging area of the eye tissue, the light beam emitted by the visual target unit, the light beam emitted by the fundus camera imaging unit and the light beam emitted by the optical coherence tomography unit are coupled to the eye ball together by the light path adjusting unit, and the light beam scattered by the eye ball is coupled to the fundus camera imaging unit, the white eye imaging unit and the optical coherence tomography unit respectively, so that the image information of different parts of the eye ball can be acquired simultaneously, thereby predicting the health condition of the human body and accurately diagnosing the eye disease.

As shown in fig. 6, in one embodiment of the present invention, the eye tissue imaging apparatus includes a sighting target unit 100, a fundus camera imaging unit 200, a white eye imaging unit 300, an optical coherence tomography unit 400, and an optical path adjusting unit 500. Wherein, the sighting target unit 100 is a light source with a visible light wave band of 400nm-650 nm; the fundus illuminating light source 220 in the fundus camera imaging unit 200 is an LED light source of 850 nm; the white eye illumination source 320 in the white eye imaging unit 300 may be 400-650nm visible light; the wavelength of the swept-frequency light source 410 in the optical coherence layer imaging unit is 1060nm or 1300nm, and the optical path adjusting unit 500 includes a long-pass dichroic mirror 520 (first dichroic mirror) of 650nm and a short-pass dichroic mirror 540 (first dichroic mirror) of 950 nm. The selection of the type of the dichroic mirror is related to the wavelength of each light source, and can be selected according to actual conditions.

Specifically, the light beam emitted from the sighting target unit 100 enters the light path adjusting unit 500 through the perspective of the beam splitter 360 (second coupling light path), is reflected by the long-pass dichroic mirror 520, reaches the short-pass dichroic mirror 540, is transmitted from the short-pass dichroic mirror 520, enters the lens group 600, and the eyeball observes the sighting target.

The fundus camera imaging process includes: the first light beam emitted by the 850nm LED light source 220 is adjusted by the illumination light beam adjustment light path 240 to form a surface illumination light beam, and the surface illumination light beam is reflected by the hollow reflector 260, enters the light path adjustment unit 500, passes through the transmission of the long-pass dichroic mirror 520 and the short-pass dichroic mirror 540 in the light path adjustment unit 500, enters the lens group 600, illuminates fundus tissues, and generates reflected light and scattered light. The reflected light and the scattered light of the fundus tissue enter the optical path adjusting unit 500 through the lens group 600, reach the fundus camera imaging unit 200 through transmission of the long-pass dichroic mirror 520 and the short-pass dichroic mirror 540 in the optical path adjusting unit 500, and enter the first imaging optical path 280 through the center hole of the hollow mirror 260, and enter the fundus imaging camera 290 for imaging through optical processing (e.g., focusing and light intensity adjustment) of the first imaging optical path 280.

The white eye imaging process comprises: the second light beam emitted by the 400-650nm visible light 320 is adjusted by the illumination light path 340 to irradiate the shallow eye surface from the side surface, and the shallow eye surface generates reflected light and scattered light. The reflected light and the scattered light enter the light path adjusting unit 500 through the lens group 600, reach the beam splitter 360 through transmission of the short-pass dichroic mirror 540 and reflection of the long-pass dichroic mirror 520, enter the second imaging light path 380 through reflection of the beam splitter 360, and then reach the white eye imaging camera 390 for imaging.

The optical coherence layer imaging process comprises: the 1060nm or 1300nm swept light source 410 emits a third light beam, which is split by the first splitting and interference light path 430 to obtain a first splitting light beam and a second splitting light beam, the first splitting light beam returns to the first splitting and interference light path 430 after being processed by the first reference light path 470, and the second splitting light beam enters the light path adjusting unit 500 after being adjusted by the first sample light path 450, enters the lens group 600 after being adjusted by the short-pass dichroic beam splitter 540 to illuminate the anterior segment and the posterior segment of the eye, and the anterior segment and the posterior segment of the eye generate reflected light and scattered light. The reflected light and the scattered light enter the light path adjusting unit 500 through the lens group 600, enter the first sample light path 450 through the reflection of the short-pass dichroic mirror 540, are processed by the first sample light path 450, and then return to the first light splitting and interference light path 430 again, the first light splitting and interference light path 430 generates interference light 1, the interference light 1 is detected by the photodetector 471, and is input to the computer 473 for display after data acquisition and processing are performed by a signal acquisition card (not shown in the figure).

It should be noted that, when the light source of the optical coherence layer imaging unit is a continuous wide-spectrum light source, the imaging process is the same as that described above, and is not described herein again.

Therefore, according to the eye tissue imaging apparatus of the embodiment of the invention, the first dichroic mirror and the second dichroic mirror couple the light beam emitted by the sighting mark unit, the light beam emitted by the fundus camera imaging unit and the light beam emitted by the optical coherence tomography unit to the eyeball together, and couple the light beam emitted by the eyeball to the fundus camera imaging unit, the white eye imaging unit and the optical coherence tomography unit respectively, so that not only can the image information of different parts of the eyeball be acquired simultaneously, the health condition of the human body be predicted, the eye disease can be diagnosed accurately, but also the corresponding light source can be turned off according to the requirement, the image information of the corresponding part of the eyeball can be acquired, and the flexibility of the eye tissue imaging apparatus is improved.

Corresponding to the above embodiment, the present invention also provides an eye tissue imaging apparatus. As shown in fig. 7, the eye tissue imaging apparatus 10 includes the eye tissue imaging device 11 described in the above embodiment.

According to the eye tissue imaging device provided by the embodiment of the invention, by implementing the eye tissue imaging device described in the above embodiment, the image information of different parts of the eyeball can be acquired simultaneously, so that the eye disease can be diagnosed accurately.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.

Furthermore, the terms "first", "second", and the like used in the embodiments of the present invention are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated in the embodiments. Thus, a feature of an embodiment of the present invention that is defined by the terms "first," "second," etc. may explicitly or implicitly indicate that at least one of the feature is included in the embodiment. In the description of the present invention, the word "plurality" means at least two or two and more, such as two, three, four, etc., unless specifically limited otherwise in the examples.

In the present invention, unless otherwise explicitly stated or limited by the relevant description or limitation, the terms "mounted," "connected," and "fixed" in the embodiments are to be understood in a broad sense, for example, the connection may be a fixed connection, a detachable connection, or an integrated connection, and it may be understood that the connection may also be a mechanical connection, an electrical connection, etc.; of course, they may be directly connected or indirectly connected through intervening media, or they may be interconnected within one another or in an interactive relationship. Those of ordinary skill in the art will understand the specific meaning of the above terms in the present invention according to their specific implementation.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It should be noted that, the technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, however, as long as there is no contradiction between the combinations of the technical features, the combinations should be considered as the scope of the present description.

Although embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are exemplary and not to be construed as limiting the present invention, and that changes, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims (10)

1. An ocular tissue imaging device, comprising:

the sighting target unit is used for adjusting the position and the angle of an eyeball;

a fundus camera imaging unit for imaging fundus tissues;

a white eye imaging unit for imaging a superficial eye surface;

the optical coherence tomography unit is used for imaging the anterior segment and the posterior segment of the eye;

and the optical path adjusting unit is used for coupling the light beam emitted by the visual target unit, the light beam emitted by the eyeground camera imaging unit and the light beam emitted by the optical coherence tomography unit to an eyeball and coupling the light beam scattered by the eyeball to the eyeground camera imaging unit, the white eye imaging unit and the optical coherence tomography unit respectively so as to acquire image information of different parts of the eyeball simultaneously.

2. The ocular tissue imaging device according to claim 1, wherein the fundus camera imaging unit comprises a fundus illumination light source, an illumination beam adjustment optical path, a first coupling optical path, a first imaging optical path, and a fundus imaging camera, wherein,

the fundus illuminating light source for providing a first light beam for illuminating fundus tissue;

the illumination light beam adjusting light path is used for adjusting the first light beam to form a surface illumination light beam;

the first coupling optical path is used for reflecting the surface illumination light beam to the optical path adjusting unit so as to be coupled to the fundus tissue through the optical path adjusting unit;

the optical path adjusting unit is further used for coupling the light beam scattered by the fundus tissue to the first imaging optical path;

the first imaging optical path is used for processing the light beam emitted by the fundus tissue coupled by the optical path adjusting unit and sending the processed light beam emitted by the fundus tissue into the fundus imaging camera for imaging.

3. The eye tissue imaging apparatus in accordance with claim 1 wherein the white eye imaging unit comprises a white eye illumination light source, an illumination light path, a second coupled light path, a second imaging light path, and a white eye imaging camera, wherein,

the white eye illumination light source for providing a second light beam for illuminating a superficial eye surface;

the illumination optical path is used for transmitting the second light beam to the superficial eye surface;

the light path adjusting unit is further used for coupling the light beam scattered by the superficial eye surface to the second coupling light path;

the second coupling optical path is used for coupling the light beam scattered by the superficial eye surface and coupled out by the optical path adjusting unit to the second imaging optical path for processing;

and the second imaging optical path is used for sending the processed light beam scattered by the superficial eye surface into the white eye imaging camera for imaging.

4. The eye tissue imaging apparatus according to claim 3, wherein the second coupling optical path is further configured to couple the light beam emitted from the target unit to the optical path adjusting unit so as to couple the light beam emitted from the target unit to the eyeball through the optical path adjusting unit.

5. The eye tissue imaging apparatus in accordance with claim 1, wherein the optical coherence tomography unit comprises a swept-frequency light source, a first split and interference optical path, a first reference optical path, a first sample optical path, and a photodetector, wherein,

the swept-frequency light source is used for providing a third light beam for illuminating the anterior segment and the posterior segment of the eye;

the first light splitting and interference optical path is used for performing light splitting processing on the third light beam so as to provide a first light splitting light beam for the first reference optical path and a second light splitting light beam for the first sample optical path;

the first reference optical path is used for reflecting the first light splitting beam and transmitting the reflected first light splitting beam to the first light splitting and interference optical path;

the first sample optical path is used for processing the second split light beam and transmitting the processed second split light beam to the optical path adjusting unit so as to couple the processed second split light beam to the anterior segment and the posterior segment of the eye through the optical path adjusting unit;

the optical path adjusting unit is further configured to couple the light beams scattered by the anterior segment and the posterior segment to the first sample optical path, and transmit the light beams scattered by the anterior segment and the posterior segment to the first light splitting and interference optical path after processing the light beams through the first sample optical path;

the first light splitting and interference light path is further configured to interfere the first light splitting beam reflected by the first reference light path with the light beams scattered from the anterior segment and the posterior segment of the eye in the first sample light path, and transmit the interfered light beams to the photodetector;

and the photoelectric detector is used for converting the interfered light beams into electric signals and carrying out subsequent spectral analysis.

6. The eye tissue imaging apparatus according to claim 1, wherein the optical coherence layer imaging unit comprises a continuous wide spectrum light source, a second split and interference optical path, a second reference optical path, a second sample optical path, a grating, a converging lens, a camera;

the wide-spectrum light source is used for providing a fourth light beam for illuminating the anterior segment and the posterior segment of the eye;

the second light splitting and interference optical path is used for splitting the fourth light beam so as to provide a third light splitting beam for the second reference optical path and a fourth light splitting beam for the second sample optical path;

the second reference light path is used for reflecting the third split light beam and transmitting the reflected third split light beam to the second light splitting and interference light path;

the second sample optical path is used for processing the fourth split beam and transmitting the processed fourth split beam to the optical path adjusting unit so as to couple the processed fourth split beam to the anterior segment and the posterior segment through the optical path adjusting unit;

the optical path adjusting unit is further configured to couple the light beams scattered by the anterior segment and the posterior segment to the second sample optical path, and the light beams scattered by the anterior segment and the posterior segment are processed by the second sample optical path and then transmitted to the second light splitting and interference optical path;

the second light splitting and interference light path is further used for interfering a third light splitting beam subjected to reflection processing by the second reference light path with light beams scattered from the anterior segment and the posterior segment of the eye of the second sample light path, and transmitting the interfered light beams to the imaging camera for spectral analysis.

7. The eye tissue imaging apparatus according to any one of claims 1-6 wherein the optical path adjustment unit comprises a first dichroic mirror and a second dichroic mirror.

8. The ocular tissue imaging device of claim 2, wherein the first coupling optical path comprises a hollow mirror.

9. The ocular tissue imaging device of claim 3, wherein the second coupled optical path comprises a beam splitter.

10. An eye tissue imaging apparatus, characterized in comprising an eye tissue imaging device according to any one of claims 1-9.

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