CN114931987A - Centrifugal micro-fluidic chip and method for synchronous detection of multiple ions in soil - Google Patents
Centrifugal micro-fluidic chip and method for synchronous detection of multiple ions in soil Download PDFInfo
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- CN114931987A CN114931987A CN202210659264.2A CN202210659264A CN114931987A CN 114931987 A CN114931987 A CN 114931987A CN 202210659264 A CN202210659264 A CN 202210659264A CN 114931987 A CN114931987 A CN 114931987A
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- 238000001514 detection method Methods 0.000 title claims abstract description 130
- 150000002500 ions Chemical class 0.000 title claims abstract description 43
- 239000002689 soil Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 182
- 238000003860 storage Methods 0.000 claims abstract description 92
- 238000006243 chemical reaction Methods 0.000 claims abstract description 51
- 230000008569 process Effects 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 38
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- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000000523 sample Substances 0.000 description 34
- 239000002086 nanomaterial Substances 0.000 description 8
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
Abstract
The invention relates to a centrifugal micro-fluidic chip and a method for synchronously detecting various ions in soil. The channel layer comprises a channel layer main body, a main storage pool of liquid to be detected and a plurality of channel branches uniformly distributed along the periphery of the main storage pool of liquid to be detected. The channel branch comprises a to-be-detected liquid auxiliary storage pool, a detection liquid storage pool, a reaction pool, a main channel and a detection pool. The liquid to be detected auxiliary storage pool is communicated with the liquid to be detected main storage pool. A capillary micro valve I is arranged between the auxiliary liquid storage pool to be detected and the detection liquid storage pool, the auxiliary liquid storage pool to be detected and the detection liquid storage pool are connected through the capillary micro valve I and then are connected with one end of the reaction pool, the other end of the reaction pool is communicated with one end of the main channel, and the other end of the main channel is connected with the detection pool through a capillary micro valve II. The invention utilizes centrifugal force to drive and control reaction, realizes synchronous detection of various soil ions, reduces manual participation process, and has the characteristics of high efficiency, convenience, accuracy and rapidness.
Description
Technical Field
The invention relates to the technical field of soil ion detection, in particular to a centrifugal micro-fluidic chip and a method for synchronously detecting various soil ions.
Background
In modern agricultural production, soil sensors develop slowly due to the complexity of soil composition, and become the focus of research in the field of agricultural sensors at present. A key problem to be solved by agricultural sensor research institutions at home and abroad is to utilize a sensor to quickly measure soil fertility in situ at a large area and in a low cost. The method for measuring parameters such as resistance, capacitance and the like of the soil by adopting the methods such as electronics, electromagnetism and the like is easily influenced by the soil composition. The soil structure, physical and chemical properties and the like can be measured by measuring the soil by using electromagnetic waves, but the absorption of the electromagnetic waves by soil components often causes large measurement errors. The electrochemical method can measure some ions in the soil, but the measuring methods are all limited, so that the in-situ measuring sensor is a difficult point for research in the international range. In order to accurately detect the information, a certain degree of breakthrough must be made in the measurement technology and the metering model.
The optical detection technology has the characteristics of high sensitivity, wide range, high system integration level and the like, particularly, the nano fluorescent detection material technology can synthesize a nano material with fluorescent wide-chromaticity evolution and wide-spectrum emission capability aiming at different target ions, and the soil ion trace detection technology which is sensitive in dosage, good in selectivity and sensitivity, stable and repeatable can be developed based on the design of a nano material probe. However, the detection mode mainly depends on laboratory operation at present, an optical instrument is used for reading signals, the operation is complicated, and the automation degree is low.
Therefore, how to construct a centrifugal microfluidic chip for synchronously detecting multiple soil ions with high automation degree, high efficiency, simplicity, convenience, accuracy and rapidness is an urgent problem to be solved in the research of soil ion in-situ detection.
Disclosure of Invention
The invention aims to provide a centrifugal micro-fluidic chip and a centrifugal micro-fluidic method for synchronously detecting various ions in soil, which can overcome the defects in the prior art, utilize centrifugal force to drive and control reaction, realize the synchronous detection of various soil ions, reduce the manual participation process, and have the characteristics of high efficiency, simplicity, convenience, accuracy and rapidness.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention relates to a centrifugal micro-fluidic chip for synchronously detecting various ions in soil.
The channel layer comprises a channel layer main body, a main storage pool of the liquid to be tested, and a plurality of channel branches, wherein the main storage pool of the liquid to be tested is arranged in the middle of the top of the channel layer main body; the channel branch comprises a to-be-detected liquid auxiliary storage pool, a detection liquid storage pool, a reaction pool, a main channel and a detection pool; the liquid to be detected auxiliary storage pool is communicated with the liquid to be detected main storage pool; a capillary micro valve I is arranged between the to-be-detected liquid auxiliary storage pool and the detection liquid storage pool, the to-be-detected liquid auxiliary storage pool and the detection liquid storage pool are connected through the capillary micro valve I and then are connected with one end of the reaction pool, the other end of the reaction pool is communicated with one end of the main channel, and the other end of the main channel is connected with the detection pool through a capillary micro valve II.
Further, the cover plate layer and the channel layer are in bonding connection.
Furthermore, a detection solution is preset in the detection solution storage pool.
Further, the cover plate layer comprises a cover plate layer main body and a to-be-detected liquid quantitative sample inlet hole formed in the middle of the cover plate layer main body; and the liquid quantitative sample inlet hole to be detected is communicated with a main storage pool of the liquid to be detected.
The cover plate layer main body is also provided with a to-be-detected liquid guide groove, a detection liquid sample inlet hole and a visual window; the number of the to-be-detected liquid diversion trenches, the number of the detection liquid sample inlet holes, the number of the visible windows and the number of the channel branches are equal; the detection liquid sample inlet holes are arranged in one-to-one correspondence with the reaction tanks and communicated with the corresponding reaction tanks; the visual windows and the detection pools are arranged in a one-to-one correspondence manner, and the visual windows are positioned right above the corresponding detection pools; one end of the liquid diversion trench to be detected is communicated with the liquid quantitative sampling hole to be detected, the other end of the liquid diversion trench is provided with a storage pool, and one side of the storage pool is provided with a vent hole communicated with the storage pool.
Further, the main passage has a spiral or serpentine shape formed by a plurality of continuous folded shapes.
Furthermore, a ventilation channel communicated with the detection pool is arranged on one side of the detection pool; the ventilation channels are arranged in one-to-one correspondence with the ventilation holes and are communicated with the corresponding ventilation holes.
Furthermore, a chip fixing hole is formed in the middle of the back of the channel layer main body.
Further, the visual window includes a through-hole opened in the cover plate layer main body and an optically transparent film installed in the through-hole.
The invention also relates to a working method of the microfluidic chip, which comprises the following steps:
(1) and (3) mounting the microfluidic chip: and the centrifugal microfluidic chip is arranged on the centrifugal detector.
(2) Injecting and flowing the liquid to be detected: adding a liquid to be detected into the liquid quantitative sample inlet hole to be detected, starting a centrifugal detector, driving the microfluidic chip to rotate for T1 seconds at a rotating speed of A1 by the centrifugal detector, wherein in the rotating process of the microfluidic chip, the liquid to be detected flows into a main liquid storage pool from the liquid quantitative sample inlet hole to be detected, then flows into each auxiliary liquid storage pool from the main liquid storage pool to be detected, and redundant liquid to be detected in the liquid quantitative sample inlet hole to be detected flows into a storage pool at the tail end of each liquid diversion trench to be detected along each liquid diversion trench to be detected; a1 has a value of 200 rpm and T1 has a value of 30.
(3) Primary mixing and reaction: increasing the rotating speed of a centrifugal detector, driving the micro-fluidic chip to rotate at the rotating speed A2 for T2 seconds by the centrifugal detector, enabling a liquid to be detected and a detection solution to flow into a reaction tank from a liquid to be detected auxiliary storage pool and a detection liquid storage pool respectively through a capillary micro valve I in the rotating process of the micro-fluidic chip, mixing the liquid to be detected and the detection solution in the reaction tank to obtain a mixed solution, and reacting ions to be detected in the liquid to be detected with the detection solution; the value of A2 was 800 rpm and the value of T2 was 10.
(4) And (3) secondary mixing and reaction: and stopping the centrifugal detector, enabling the mixed liquid in the reaction tank to flow into the main channel, further mixing and reacting the liquid to be detected in the mixed liquid and the detection solution in the main channel, and flowing to a second capillary micro valve between the main channel and the detection tank.
(5) Mixing and reaction completion: and starting the centrifugal detector again, wherein the centrifugal detector drives the micro-fluidic chip to rotate for T3 seconds at the rotating speed of A3, and in the rotating process of the micro-fluidic chip, the mixed liquid between the main channel and the detection pool flows into the detection pool through the second capillary micro valve. A3 was 1500 rpm and T3 was 20.
Furthermore, detection solutions for detecting different ions are preset in different detection solution storage pools.
Compared with the prior art, the invention has the advantages that:
the centrifugal micro-fluidic chip and the method for synchronously detecting various ions in soil solve the problem of flow control of a liquid to be detected and a detection liquid by using a multi-stage capillary micro-valve structure, realize accurate controllability of reaction and detection processes by using centrifugal force driving, and solve the problems that in-situ detection is difficult to automatically, simply, accurately and quickly, so that the technical effect of on-site quick detection for instantly obtaining detection data after a sample is injected into a system can be realized. The probe is made of nano materials, the probe is combined with microfluidics to synchronously detect various ions in the soil, and the species and concentration information of the ions in the soil can be obtained in real time by utilizing the specific reaction of the nano materials to the ions and the fluorescence change before and after the reaction. The technology has the ion specificity recognition capability, effectively solves the problem of synchronous detection of various ions in soil, and simultaneously can eliminate visible light wave band interference and improve the sensitivity and reliability of ion detection by using a fluorescence detection method. The centrifugal micro-fluidic chip provided by the invention realizes synchronous detection of various ions in soil, can efficiently, simply, accurately and quickly obtain the information of the ion types and the ion concentrations in the soil, and enriches and develops the automatic acquisition and sensing technology of modern agricultural sensors.
Drawings
FIG. 1 is a schematic diagram of a centrifugal microfluidic chip according to the present invention;
FIG. 2 is a schematic structural view of a cover sheet layer of the present invention;
fig. 3 is a schematic structural diagram of a channel layer in the present invention.
Wherein:
1. the device comprises a cover plate layer 11, a to-be-detected liquid quantitative sample inlet hole 12, a to-be-detected liquid diversion trench 13, a detection liquid sample inlet hole 14, a vent hole 15, a visual window 2, a channel layer 21, a to-be-detected liquid main storage pool 22, a to-be-detected liquid auxiliary storage pool 23, a detection liquid storage pool 24, a main channel 25, a capillary micro valve I, a capillary micro valve 26, a reaction pool 27, a detection pool 28, a ventilation channel 29 and a capillary micro valve II.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
the centrifugal microfluidic chip for synchronously detecting multiple ions in soil as shown in fig. 1 comprises a cover plate layer 1 and a channel layer 2 which are arranged in sequence; the cover plate layer 1 and the channel layer 2 are bonded and connected.
As shown in fig. 1 and 2, the cover plate layer 1 includes a cover plate layer main body and a quantitative sample injection hole 11 for a liquid to be measured, which is opened in the middle of the cover plate layer main body. The cover plate layer main body is also provided with a to-be-detected liquid diversion trench 12, a detection liquid sample inlet hole 13 and a visible window 15. The number of the liquid guide grooves 12 to be detected, the number of the detection liquid sample inlet holes 13, the number of the visual windows 15 and the number of the channel branches are equal. The viewing window 15 includes a through-hole opened in the cover sheet layer main body and an optically transparent film installed in the through-hole. The liquid quantitative sample inlet 11 is communicated with a main liquid storage tank 21. The detection liquid sample inlet holes 13 are arranged in one-to-one correspondence with the reaction tanks 26, and the detection liquid sample inlet holes 13 are communicated with the corresponding reaction tanks 26; the visual windows 15 are arranged corresponding to the detection pools 27 one by one, and the visual windows 15 are positioned right above the corresponding detection pools 27; one end of the liquid diversion trench 12 to be detected is communicated with the liquid quantitative sampling hole 13 to be detected, the other end is provided with a storage pool 21, and one side of the storage pool 21 is provided with a vent hole 14 communicated with the storage pool 21.
The quantitative sample inlet hole 11 for the liquid to be detected is a sample inlet position of the liquid to be detected of the microfluidic chip, is designed to be but not limited to a cylindrical structure, can contain a certain amount of the liquid to be detected, and is vertically communicated with the main storage pool 21 for the liquid to be detected in the channel layer 2, so that the purpose that the liquid to be detected enters the channel layer is achieved. The liquid guide grooves 12 to be detected are uniformly distributed along the liquid quantitative sampling hole 11 in a radiation manner, the liquid guide grooves 12 to be detected are of a micro-pipeline structure and can accommodate the redundant liquid to be detected which overflows the liquid quantitative sampling hole 11 to be detected, and the liquid to be detected is guided into the storage tank 21 at the tail end, so that the quantitative sampling of the liquid to be detected is realized. The detection liquid sample inlet holes 13 are arranged in one-to-one correspondence with the detection liquid storage pools 23 on the channel layer 2 and are communicated up and down, and after the cover plate layer 1 and the channel layer 2 of the microfluidic chip are sealed and bonded, detection solution containing nano probe materials is preset in the detection liquid storage pools 23 of the channel layer 2 through the detection liquid sample inlet holes 13. The vent holes 14 are communicated with vent channels 28 on the channel layer up and down and are used for air pressure balance in all areas of the microfluidic chip. The visual windows 15 correspond to the detection cells of the channel layer one by one and are detection channels of the fluorescence emission and receiving device, and the detection region is sealed and the fluorescence detection performance is improved by attaching an optical transparent film.
As shown in fig. 1 and fig. 3, the channel layer 2 includes a channel layer main body, a main storage pool 21 of liquid to be measured disposed in the middle of the top of the channel layer main body, and a plurality of channel branches disposed on the top of the channel layer main body and uniformly distributed along the periphery of the main storage pool 21 of liquid to be measured; the channel branches comprise a to-be-detected liquid auxiliary storage pool 22, a detection liquid storage pool 23, a reaction pool 26, a main channel 24 and a detection pool 27. The auxiliary storage tank 22 of the liquid to be tested is communicated with the main storage tank 21 of the liquid to be tested; a capillary micro valve I25 is arranged between the to-be-detected liquid auxiliary storage pool 22 and the detection liquid storage pool 23, the to-be-detected liquid auxiliary storage pool 22 and the detection liquid storage pool 23 are connected through the capillary micro valve I25 and then are connected with one end of a reaction pool 26, the other end of the reaction pool 26 is communicated with one end of a main channel 24, and the other end of the main channel 24 is connected with a detection pool 27 through a capillary micro valve II 29. The main passage 24 has a spiral or serpentine shape formed by a plurality of continuous folded shapes. A ventilation channel 28 communicated with the detection cell 27 is arranged on one side of the detection cell 27; the vent passages 28 are arranged in one-to-one correspondence with the vent holes 14, and the vent passages 28 are communicated with the corresponding vent holes 14. And a chip fixing hole is formed in the middle of the back of the channel layer main body. The chip fixing hole is positioned on the back of the channel layer main body structure layer. The main structure of the channel layer comprises a main storage pool 21 of liquid to be detected, an auxiliary storage pool 22 of liquid to be detected, a detection liquid storage pool 23, a main channel 24, a first capillary micro valve 25, a second capillary micro valve 29, a reaction pool 26, a detection pool 27 and a ventilation channel 28, the main structure of the channel layer is uniform in thickness and distributed on one side of the channel layer, the other side of the channel layer is designed to be used for a chip fixing hole structure for connecting a micro-fluidic chip and a centrifugal detector, the chip fixing hole corresponds to the centrifugal detector fixing position structure, the thickness does not penetrate through the channel layer, and the main structure of the other side of the channel layer cannot be damaged.
The detection solution storage tank 23 is provided with a detection solution in advance. Detection solutions for detecting different ions are preset in different detection solution storage pools and are used for detecting different ions. The detection solution comprises a probe of a fluorescent nano material with specific identification capability and a corresponding solvent, wherein the probe of the fluorescent nano material is uniformly dispersed in the solvent and has specific fluorescence excitation and emission wavelengths. After the detection solution and the solution to be detected are mixed, the probe for detecting the nano material in the solution can identify the ions to be detected, so that the fluorescence wavelength is rapidly and obviously changed, and the in-situ quantitative detection can be realized by combining the detection part. The probe of the nano material adopts a chemical organic synthesis method, synthesizes nano probes with different structures and specificity identification capability aiming at different ions, and has the characteristics of high selectivity, high sensitivity, visual quantitative detection and the like.
And the main storage pool 21 of the liquid to be detected is used for accommodating the liquid to be detected in the channel layer and uniformly distributing the liquid to be detected to the surrounding channel branches. And the to-be-detected liquid auxiliary storage pool 22 is used for containing a fixed volume of to-be-detected liquid, is symmetrically distributed along the to-be-detected liquid main storage pool, and can realize the accurate control of the to-be-detected liquid participating in the reaction under the centrifugal driving action. And a detection solution reservoir 23 for containing a fixed volume of detection solution, which is a solution containing a nano probe material and is stored therein in a preset manner. A first capillary micro valve 25 structure is designed between the detection liquid storage tank 23, the auxiliary liquid storage tank 22 to be detected and the reaction tank 26, and the two solutions are retained at the front end of the first capillary micro valve 25 before being mixed. The main channel 24 is used for further improving the mixing and reaction of the liquid to be detected and the detection solution, one end of the main channel is connected with the reaction tank 26, and the mixed liquid which completes the reaction in the reaction tank 26 directly enters the main channel 24 under the action of capillary force; the other end is connected with the detection pool 27 through a second capillary micro valve 29 structure, and the mixed liquid which is fully mixed and reacted in the main channel 24 is intercepted at the front end of the second capillary micro valve 29. The first capillary micro valve 25 and the second capillary micro valve 29 are two passive micro valves, and the structure is used for completing the flow control of liquid in the micro-fluidic chip. Under the driving of centrifugal force, the internal pressure of the liquid breaks through the capillary micro valve and enters the next area, so that the working mode of the centrifugal force-driven micro-fluidic chip is realized. The first capillary micro valve 25 and the second capillary micro valve 29 have different structural sizes, and the liquid interception performance of the capillary micro valve can be broken through only by driving at different centrifugal rotating speeds. And the liquid to be detected and the detection solution break through the structure of the first capillary micro valve 25 under the driving of centrifugal force and enter the reaction tank 26, the flow rate of the liquid is slow in a micro-fluidic environment, and the two solutions are mixed and react in the process of filling the reaction tank. The mixed solution which finishes the reaction is driven by centrifugal force to pass through the second capillary micro valve 29 between the main channel 24 and the detection cell 27, slowly fills and stays in the area of the detection cell 27, and waits for fluorescence detection. The vent channel 28 is communicated with the corresponding vent hole 14 on the cover plate layer 1 up and down, and is designed in an open mode, so that the air pressure balance of each area of the microfluidic chip is kept.
The invention also relates to a working method of the centrifugal microfluidic chip for synchronously detecting the multiple ions in the soil, which comprises the following steps:
(1) and (3) mounting the microfluidic chip: and the centrifugal microfluidic chip is arranged on a centrifugal detector.
(2) Injecting and flowing liquid to be detected: adding a liquid to be detected into the liquid quantitative sample inlet hole 11 to be detected, starting a centrifugal detector, driving a micro-fluidic chip to rotate for T1 seconds at a rotating speed of A1 by the centrifugal detector, wherein in the rotating process of the micro-fluidic chip, the liquid to be detected flows into a main storage pool 21 of the liquid to be detected from the liquid quantitative sample inlet hole 11 to be detected, then flows into each auxiliary storage pool 22 of the liquid to be detected from the main storage pool 21 of the liquid to be detected, and redundant liquid to be detected in the liquid quantitative sample inlet hole 11 to be detected flows into a storage pool 16 at the tail end of each guide groove 12 of the liquid to be detected along each guide groove 12 of the liquid to be detected; a1 was 200 rpm and T1 was 30.
(3) Primary mixing and reaction: increasing the rotating speed of a centrifugal detector, wherein the centrifugal detector drives the micro-fluidic chip to rotate at the rotating speed A2 for T2 seconds, in the rotating process of the micro-fluidic chip, the liquid to be detected and the detection solution respectively flow from the auxiliary storage pool 22 of the liquid to be detected and the storage pool 23 of the detection solution to the reaction pool 26 through the first capillary micro valve 25, in the reaction pool 26, the liquid to be detected and the detection solution are mixed to obtain a mixed solution, and ions to be detected in the liquid to be detected react with the detection solution; a2 has a value of 800 rpm and T2 has a value of 10.
(4) And (3) secondary mixing and reaction: the centrifugal detector stops working, the mixed liquid in the reaction pool 26 flows into the main channel 24, the liquid to be detected in the mixed liquid in the main channel 24 is further mixed and reacted with the detection solution, and the liquid flows to the second capillary micro valve 29 between the main channel 24 and the detection pool 27.
(5) Mixing and reaction completion: and starting the centrifugal detector again, wherein the centrifugal detector drives the micro-fluidic chip to rotate for T3 seconds at the rotating speed of A3, and in the rotating process of the micro-fluidic chip, the mixed liquid between the main channel 24 and the detection pool 27 flows into the detection pool 27 through the second capillary micro valve 29. A3 was 1500 rpm and T3 was 20.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (10)
1. A centrifugal micro-fluidic chip for multiple ion synchronous detection of soil, its characterized in that: the device comprises a cover plate layer and a channel layer which are arranged in sequence;
the channel layer comprises a channel layer main body, a main storage pool of the liquid to be detected, and a plurality of channel branches, wherein the main storage pool of the liquid to be detected is arranged in the middle of the top of the channel layer main body, and the channel branches are arranged at the top of the channel layer main body and are uniformly distributed along the periphery of the main storage pool of the liquid to be detected; the channel branch comprises a to-be-detected liquid auxiliary storage pool, a detection liquid storage pool, a reaction pool, a main channel and a detection pool; the liquid to be detected auxiliary storage pool is communicated with the liquid to be detected main storage pool; a capillary micro valve I is arranged between the to-be-detected liquid auxiliary storage pool and the detection liquid storage pool, the to-be-detected liquid auxiliary storage pool and the detection liquid storage pool are connected through the capillary micro valve I and then are connected with one end of the reaction pool, the other end of the reaction pool is communicated with one end of the main channel, and the other end of the main channel is connected with the detection pool through a capillary micro valve II.
2. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 1, wherein: the cover plate layer and the channel layer are in bonding connection.
3. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 1, wherein: and a detection solution is preset in the detection solution storage pool.
4. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 1, wherein: the cover plate layer comprises a cover plate layer main body and a to-be-detected liquid quantitative sample inlet hole formed in the middle of the cover plate layer main body; the liquid quantitative sample inlet hole to be detected is communicated with a main storage pool of the liquid to be detected;
the cover plate layer main body is also provided with a to-be-detected liquid guide groove, a detection liquid sample inlet hole and a visual window; the number of the to-be-detected liquid diversion grooves, the number of the detection liquid sample inlet holes, the number of the visual windows and the number of the channel branches are equal; the detection liquid sample inlet holes are arranged in one-to-one correspondence with the reaction tanks and communicated with the corresponding reaction tanks; the visual windows and the detection pools are arranged in a one-to-one correspondence manner, and the visual windows are positioned right above the corresponding detection pools; one end of the liquid diversion trench to be detected is communicated with the liquid quantitative sampling hole to be detected, the other end of the liquid diversion trench is provided with a storage pool, and one side of the storage pool is provided with a vent hole communicated with the storage pool.
5. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 1, wherein: the main passage has a spiral or serpentine shape formed by a plurality of continuous folded shapes.
6. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 4, wherein: a ventilation channel communicated with the detection pool is arranged on one side of the detection pool; the ventilation channels are arranged in one-to-one correspondence with the ventilation holes and are communicated with the corresponding ventilation holes.
7. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 1, wherein: and a chip fixing hole is formed in the middle of the back of the channel layer main body.
8. The centrifugal microfluidic chip for synchronous detection of multiple ions in soil according to claim 4, wherein: the visual window includes a through-hole opened in the cover plate layer main body and an optically-transparent film installed in the through-hole.
9. The method of any one of claims 1 to 8, wherein the method comprises the following steps: the method comprises the following steps:
(1) and (3) mounting the microfluidic chip: mounting the centrifugal microfluidic chip on a centrifugal detector;
(2) injecting and flowing liquid to be detected: adding a liquid to be detected into the liquid quantitative sample inlet hole to be detected, starting a centrifugal detector, driving the microfluidic chip to rotate for T1 seconds at a rotating speed of A1 by the centrifugal detector, wherein in the rotating process of the microfluidic chip, the liquid to be detected flows into a main liquid storage pool from the liquid quantitative sample inlet hole to be detected, then flows into each auxiliary liquid storage pool from the main liquid storage pool to be detected, and redundant liquid to be detected in the liquid quantitative sample inlet hole to be detected flows into a storage pool at the tail end of each liquid diversion trench to be detected along each liquid diversion trench to be detected;
(3) primary mixing and reaction: increasing the rotating speed of a centrifugal detector, driving the micro-fluidic chip to rotate at the rotating speed A2 for T2 seconds by the centrifugal detector, enabling a liquid to be detected and a detection solution to flow into a reaction tank from a liquid to be detected auxiliary storage pool and a detection liquid storage pool respectively through a capillary micro valve I in the rotating process of the micro-fluidic chip, mixing the liquid to be detected and the detection solution in the reaction tank to obtain a mixed solution, and reacting ions to be detected in the liquid to be detected with the detection solution;
(4) and (3) secondary mixing and reacting: the centrifugal detector stops working, the mixed liquid in the reaction tank flows into the main channel, the liquid to be detected in the mixed liquid in the main channel is further mixed and reacted with the detection solution, and the liquid flows to a second capillary micro valve between the main channel and the detection tank;
(5) mixing and reaction are completed: and starting the centrifugal detector again, wherein the centrifugal detector drives the micro-fluidic chip to rotate for T3 seconds at the rotating speed of A3, and in the rotating process of the micro-fluidic chip, the mixed liquid between the main channel and the detection pool flows into the detection pool through the second capillary micro valve.
10. The method of claim 9, wherein: detection solutions for detecting different ions are preset in different detection solution storage pools.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210659264.2A CN114931987A (en) | 2022-06-13 | 2022-06-13 | Centrifugal micro-fluidic chip and method for synchronous detection of multiple ions in soil |
| PCT/CN2022/133847 WO2023240930A1 (en) | 2022-06-13 | 2022-11-23 | Soil nutrient field test device, test method thereof, and micro-fluidic chip |
| US18/396,333 US20240125757A1 (en) | 2022-06-13 | 2023-12-26 | Device and method for detecting soil nutrients on site and microfluidic chip |
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| CN202210659264.2A CN114931987A (en) | 2022-06-13 | 2022-06-13 | Centrifugal micro-fluidic chip and method for synchronous detection of multiple ions in soil |
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| CN114931987A true CN114931987A (en) | 2022-08-23 |
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| WO2023240930A1 (en) * | 2022-06-13 | 2023-12-21 | 中科合肥智慧农业协同创新研究院 | Soil nutrient field test device, test method thereof, and micro-fluidic chip |
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