CN113358619B - Preparation method and application of fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution - Google Patents
Preparation method and application of fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution Download PDFInfo
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Abstract
The invention provides a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, the analysis card is prepared from a Polydimethylsiloxane (PDMS) reaction substrate, a standard fluorescence color-developing card and a reaction module, wherein the reaction module is an array formed by uniformly distributed reagent grooves and sample groove structures, the reagent grooves are connected through drainage grooves, the reaction module is fixed at one end of the polydimethylsiloxane reaction substrate, the standard fluorescence color-developing card is fixed at the other end of the polydimethylsiloxane reaction substrate, paper-based sensing materials are filled in the reagent grooves, the solution to be detected is filled in the liquid groove to be detected, fluorescence change information on the reaction module corresponds to the standard fluorescence color-developing card one by one, when the analysis card is used, the potassium permanganate solution is added in the sample groove, the potassium permanganate solution respectively enters the reagent grooves through the drainage grooves, after 20s of the reaction, the concentration in the standard fluorescence color-developing card is compared with fluorescence of the paper-based sensing materials through 365nm ultraviolet irradiation, and the semi-quantitatively detecting of the potassium permanganate solution is realized. The defects of sequential dripping and poor detection timeliness of reagents are overcome, and the detection analysis efficiency is improved.
Description
Technical Field
The invention belongs to the field of explosive residue detection and analysis, and particularly relates to a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting a potassium permanganate solution.
Background
KMnO 4 The main existence form in the environment is liquid, and excessive discharge can lead to water pollution, thereby realizing KMnO 4 Solution detection is very important for environmental protection. At present, the detection method comprises a colorimetric method by means of an optical microscope, a flame atomic absorption method and the like, and is not only applied to expensive and precise instruments, but also requires complicated steps of sample pretreatment, signal contrast analysis and the like in the detection process, so that the on-site, rapid and visual recognition requirements are not facilitated. If the method can collect sampling, simultaneous contact with all detection reagents, fluorescence information change, color change information and result descriptionThe integrated potassium permanganate residual analysis device is soft in material, small in size and easy to carry, and the detection efficiency and accuracy of the potassium permanganate residual analysis device are greatly improved.
Disclosure of Invention
The invention aims to provide a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, wherein the analysis card is prepared from a Polydimethylsiloxane (PDMS) reaction substrate, a standard fluorescence color-developing card and a reaction module, wherein the reaction module is an array formed by uniformly distributed reagent grooves and sample groove structures, the reagent grooves and the sample grooves are respectively connected through drainage grooves, the reaction module is fixed at one end of the polydimethylsiloxane reaction substrate, the standard fluorescence color-developing card is fixed at the other end of the polydimethylsiloxane reaction substrate, a paper-based sensing material loaded with coumarin probes is filled in the reagent grooves, the sample grooves are filled with a solution to be detected, and fluorescence change information on the paper-based sensing material in the reagent grooves corresponds to the standard fluorescence color-developing card one by one. When the kit is used, the potassium permanganate solution is added into the liquid tank to be detected, the potassium permanganate solution is respectively fed into the reagent tank through the drainage tank, after the reaction is carried out for 20 seconds, the fluorescent images in the paper-based sensing material fluorescent and standard fluorescent color card are compared through 365nm ultraviolet irradiation, and the semi-quantitative detection of the 5-400 mu M potassium permanganate solution is realized. The defects of sequential dripping and poor detection timeliness of reagents are overcome, and the detection analysis efficiency is improved.
The invention relates to a preparation method of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, which comprises a polydimethylsiloxane reaction substrate (1), a standard fluorescence color-developing card (2) and a reaction module (3), wherein the reaction module (3) is an array formed by uniformly distributing 6 reagent grooves (4) with the diameter of 6.5mm and the depth of 2mm and sample grooves (6) with the diameter of 1cm and the depth of 1mm, the reagent grooves (4) are connected with the sample grooves (6) through drainage grooves (5), the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction substrate (1), the standard fluorescence color-developing card (2) is fixed at the other end of the polydimethylsiloxane reaction substrate (1), paper-based sensing materials (7) loaded with 50-500 mu M coumarin-based probe molecules of potassium permanganate are filled in the 6 reagent grooves (4), the sample grooves (6) are filled with to-be-detected solution, and fluorescence change information on the reaction module (3) corresponds to the standard fluorescence color-developing card (2) one by one, and the following steps are carried out:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a rectangular template (8) which is made of resin and has a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution for 30 minutes, placing the standard fluorescent color card (2) which is printed in the surface of the polydimethylsiloxane solution slowly after placing the polydimethylsiloxane solution in a 10-minute period, placing the polydimethylsiloxane solution in the rectangular template (8) again after taking 5ml, placing the rectangular template (8) in an oven at a temperature of 60-70 ℃, and taking out the polydimethylsiloxane template after 3-5 hours to obtain the formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, naturally airing after 1 minute, and obtaining a paper-based sensing material (7) of 50-500 mu M coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. and c, respectively putting the paper-based sensing materials (7) of the 50-500 mu M coumarin-based probe molecules obtained in the step b into 6 reagent tanks (4) with the diameter of 6.5mm one by one, and obtaining the fluorescent sensing analysis card of the semi-quantitative potassium permanganate solution.
The method comprises the steps of adding 5-400 mu M potassium permanganate solution into a sample groove (6) with the diameter of 1cm, respectively introducing the 5-400 mu M potassium permanganate solution into a reagent groove (4) with the diameter of 6.5mm through a drainage groove (5), waiting for the detection of the paper-based sensing material (7) of coumarin-based probe molecules in the reagent groove (4) to fully contact with the detection of the potassium permanganate solution for 20s, and comparing fluorescence of the paper-based sensing material (7) of the coumarin-based probe molecules with fluorescence images of the standard fluorescent color card (2) after adding potassium permanganate with different concentrations through 365nm ultraviolet light irradiation, wherein the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard color card (2) to obtain the approximate concentration of the potassium permanganate, namely the 5-400 mu M potassium permanganate solution is semi-quantitatively detected.
The invention relates to a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, wherein the fluorescence sensing analysis card obtained by the method comprises the following steps of detecting 5-400 mu M potassium permanganate solution:
adding a to-be-detected liquid potassium permanganate solution into a sample groove (6) with the diameter of 1cm in a fixed reaction module (3) at one end of a polydimethylsiloxane reaction substrate (1), respectively flowing the to-be-detected liquid potassium permanganate solution into a reagent groove (4) with 6 connected built-in paper-based sensing materials (7) along a drainage groove (5) with an inclined angle, then ensuring that the to-be-detected liquid potassium permanganate solution is uniformly distributed in the reagent groove (4), waiting for the to-be-detected potassium permanganate solution to fully contact with the paper-based sensing materials (7) in the reagent groove (4) for 20 seconds, irradiating a reaction area by utilizing a 365nm ultraviolet lamp, and performing checking comparison with fluorescent color information of a standard fluorescent color development card (2) fixed at the other end of the polydimethylsiloxane reaction substrate (1), thereby determining the content range of the potassium permanganate solution in the to-be-detected. The rapid semi-quantitative identification and the operation comparison integration function can be realized. The fluorescence sensing analysis card provided by the invention has the advantages of soft material, stable physical and chemical properties, small volume and portability, greatly reduces the operation time and the detection time, and improves the detection analysis efficiency and the accuracy of the test result. The defects of sequential dripping or mixed use of detection reagents, sequence analysis execution and poor detection timeliness of the sample of the object to be detected are overcome.
Compared with the prior art, the invention has the following beneficial effects:
(1) An array consisting of 6 reagent tanks and 1 sample tank structure capable of placing paper-based sensing materials with different concentrations is constructed, semi-quantitative detection can be realized by one-time sample addition, and the defects of sequential reagent dropwise addition and poor detection timeliness are overcome.
(2) The standard fluorescent color-developing card arranged in the fluorescent sensing analysis card mainly contains fluorescent color-changing information, so that sample adding, fluorescent color developing and identifying integration can be truly realized, and the detection and analysis efficiency is improved.
(3) The fluorescence sensing analysis card disclosed by the invention is mainly made of PDMS, has the bending degree of 180 degrees, is hydrophobic and stable, is not easily influenced by environmental temperature and humidity changes, and is also greatly convenient to carry.
(4) The detection time is not longer than 20 seconds, and the detection and identification process is completed rapidly.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of a template for molding a Polydimethylsiloxane (PDMS) substrate of the present invention.
Detailed Description
Further description is provided below with reference to the accompanying drawings.
Example 1
The invention relates to a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, the analysis card consists of a polydimethylsiloxane reaction substrate 1, a standard fluorescence color-developing card 2 and a reaction module 3, wherein the reaction module 3 is an array formed by uniformly distributed 6 reagent grooves 4 with the diameter of 6.5mm and the depth of 2mm and sample grooves 6 with the diameter of 1cm and the depth of 1mm, the reagent grooves 4 and the sample grooves 6 are connected through a drainage groove 5, the reaction module 3 is fixed at one end of the polydimethylsiloxane reaction substrate 1, the standard fluorescence color-developing card 2 is fixed at the other end of the polydimethylsiloxane reaction substrate 1, paper-based sensing materials 7 loaded with 50-500 mu M coumarin-based probe molecules of potassium permanganate are filled in the 6 reagent grooves 4, the sample grooves 6 are filled with solution to be detected, and fluorescence change information on the reaction module 3 corresponds to the standard fluorescence color-developing card 2 one by one, and the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in a baking oven with a temperature of 60 ℃ for 5 hours, and taking out the cuboid template 8 to obtain the formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, and respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules, 5 mu M potassium permanganate solution is added into a sample tank 6 with the diameter of 1cm, 5 mu M potassium permanganate solution is respectively introduced into a reagent tank 4 with the diameter of 6.5mm through a drainage tank 5, the 5 mu M potassium permanganate solution to be measured is waited to be fully contacted with 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules in the reagent tank 4 for 20 seconds, and then the fluorescence of the paper-based sensing material 7 of coumarin-based probe molecules is compared with the fluorescence image after the potassium permanganate solution with different concentrations in a standard fluorescent color card 2 is added by 365nm ultraviolet light, and the fluorescence change in the paper-based sensing material 7 is compared with the fluorescence change in the standard color card 2 by 10 mu M KMnO in the standard color card 2 4 The fluorescence change of (2) is similar to that of the first row of the standard fluorescence colorimetric card, and the obtained potassium permanganate approximately has the concentration of less than 10 mu M.
Example 2
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in an oven with a temperature of 70 ℃, and taking out the cuboid template after 3 hours to obtain the formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, and respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50. Mu.M, 100. Mu.M, 200. Mu.M, 300. Mu.M, 400. Mu.M and 500. Mu.M of the paper-based sensing material 7 of coumarin-based probe molecules, 80. Mu.M of potassium permanganate solution is added to a sample tank 6 having a diameter of 1cm, the 80. Mu.M of potassium permanganate solution is introduced into a reagent tank 4 having a diameter of 6.5mm through a drainage tank 5, and after the 80. Mu.M of potassium permanganate solution of a sample is sufficiently contacted with the 50. Mu.M, 100. Mu.M, 200. Mu.M, 300. Mu.M, 400. Mu.M and 500. Mu.M of the paper-based sensing material 7 of coumarin-based probe molecules in the reagent tank 4, the coumarin is compared by irradiation with 365nm ultraviolet lightFluorescent images after the fluorescence of the paper-based sensing material 7 based on probe molecules and the potassium permanganate with different concentrations in the standard fluorescent color card 2 are added, the fluorescence change and the gradual enhanced blue fluorescence of the paper-based sensing material 7 are shown, and the fluorescence color is 50 mu M or 100 mu M KMnO in the standard color card 2 4 The fluorescence change of the potassium permanganate is obtained, and the approximate concentration is 50-100 mu M.
Example 3
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in an oven with a temperature of 65 ℃ for 4 hours, and taking out the cuboid template 8 to obtain the formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, and respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50. Mu.M, 100. Mu.M, 200Mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecules of paper-based sensing material 7, 100 mu M potassium permanganate solution is added into a sample tank 6 with the diameter of 1cm, 100 mu M potassium permanganate solution is respectively fed into a reagent tank 4 with the diameter of 6.5mm through a drainage tank 5, 100 mu M potassium permanganate solution of a measured object is waited to be fully contacted with 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecules of paper-based sensing material 7 in the reagent tank 4 for 20 seconds, 365nm ultraviolet irradiation is adopted, fluorescence of the coumarin-based probe molecules of paper-based sensing material 7 is compared with fluorescence images of the added potassium permanganate with different concentrations in a standard fluorescent color card 2, and fluorescence change in the paper-based sensing material 7 shows a relatively obvious blue fluorescence enhancement trend and is compared with 100 mu M KMnO in the standard color card 2 4 The fluorescence change of the potassium permanganate is corresponding to the concentration of 100 mu M.
Example 4
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in a baking oven with a temperature of 60 ℃ for 5 hours, and taking out the cuboid template 8 to obtain the formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, and respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules, 160 mu M potassium permanganate solution is added into a sample tank 6 with the diameter of 1cm, 160 mu M potassium permanganate solution is respectively introduced into a reagent tank 4 with the diameter of 6.5mm through a drainage tank 5, 160 mu M potassium permanganate solution of an object to be detected is equal to 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules in the reagent tank 4, after the paper-based sensing material 7 of coumarin-based probe molecules is fully contacted with 20s, the fluorescence of the paper-based sensing material 7 of coumarin-based probe molecules is compared with fluorescence images after the addition of potassium permanganate with different concentrations in a standard fluorescent color card 2 through 365nm ultraviolet light, and fluorescence change and gradual enhanced strong blue fluorescence are displayed in the paper-based sensing material 7, and KMnO is 100 and 200 mu M in the standard color card 2 4 The potassium permanganate approximately has the concentration of 100-200 mu M.
Example 5
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in an oven with a temperature of 63 ℃ for 5 hours, and taking out the cuboid template 8 to obtain the formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers with the diameter of 6mm by using a puncher, and respectively soaking the obtained 6 wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules, 266 mu M potassium permanganate solution is added into a sample tank 6 with the diameter of 1cm, 266 mu M potassium permanganate solution is respectively introduced into a reagent tank 4 with the diameter of 6.5mm through a drainage tank 5, 266 mu M potassium permanganate solution of an object to be detected is fully contacted with 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules in the reagent tank 4 for 20 seconds, by 365nm ultraviolet irradiation, fluorescence change in the paper-based sensing material 7 of coumarin-based probe molecules and stronger blue fluorescence which is gradually enhanced are compared with fluorescence images after adding potassium permanganate with different concentrations in a standard fluorescent color card 2, and fluorescence change in the paper-based sensing material 7 is between 200 mu M and 300 mu M KMnO in the standard color card 2 4 The potassium permanganate approximately has the concentration of 200-300 mu M.
Example 6
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a beaker, slowly pouring 5ml of the polydimethylsiloxane solution in a cuboid template 8 made of resin and having a length of 8cm, a width of 4cm and a depth of 3mm after placing the polydimethylsiloxane solution in the beaker for 30 minutes, slowly placing the printed standard fluorescent color card 2 in the surface of the polydimethylsiloxane solution after placing the polydimethylsiloxane solution in the beaker for 10 minutes, pouring 5ml of the polydimethylsiloxane solution in the cuboid template 8, placing the cuboid template 8 in an oven with a temperature of 70 ℃ for 5 hours, and taking out the cuboid template 8 to obtain the formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6mm wafers by using a puncher, and soaking the obtained wafers in 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule solutions one by one, naturally airing after 1 minute to obtain 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M coumarin-based probe molecule-loaded paper-based sensing material 7;
preparing a fluorescence sensing analysis card:
c. b, placing the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M obtained in the step b into 6 reagent tanks 4 with the diameter of 6.5mm one by one, and obtaining a fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution;
in use, according to 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules, 400 mu M potassium permanganate solution is added into a sample tank 6 with the diameter of 1cm, 400 mu M potassium permanganate solution is respectively introduced into a reagent tank 4 with the diameter of 6.5mm through a drainage tank 5, the 400 mu M potassium permanganate solution of an object to be detected is fully contacted with 50 mu M,100 mu M,200 mu M,300 mu M,400 mu M and 500 mu M of paper-based sensing material 7 of coumarin-based probe molecules in the reagent tank 4 for 20 seconds, after the paper-based sensing material 7 of coumarin-based probe molecules is irradiated by 365nm ultraviolet light, fluorescence of the paper-based sensing material 7 of coumarin-based probe molecules is compared with fluorescence images after the potassium permanganate solution with different concentrations in a standard fluorescent color card 2 is added, fluorescence change in the paper-based sensing material 7 shows gradually enhanced very strong blue fluorescence, and the fluorescence of KMnO is gradually enhanced with 400 mu M in the standard color card 2 4 The fluorescence change of (2) is close to that of the potassium permanganate, and the concentration of the potassium permanganate is approximately 400 mu M.
The above is a preferred embodiment of the present invention, and all changes made according to the technical solution of the present invention belong to the protection scope of the present invention when the generated functional effects do not exceed the scope of the technical solution of the present invention.
Claims (2)
1. The preparation method of the fluorescence sensing analysis card for semi-quantitatively detecting the potassium permanganate solution is characterized in that the analysis card consists of a polydimethylsiloxane reaction substrate (1), a standard fluorescence color-developing card (2) and a reaction module (3), wherein the reaction module (3) is an array formed by uniformly distributing 6 reagent tanks (4) with the diameters of 6.5mm and the depths of 2mm and sample tanks (6) with the diameters of 1cm and the depths of 1mm, the reagent tanks (4) and the sample tanks (6) are connected through a drainage tank (5), the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction substrate (1), the standard fluorescence color-developing card (2) is fixed at the other end of the polydimethylsiloxane reaction substrate (1), paper-based sensing materials (7) loaded with 50-500 mu M coumarin-based probe molecules of potassium permanganate are respectively filled in the 6 reagent tanks (4), the sample tanks (6) are filled with to-be-detected solution, fluorescence change information on the reaction module (3) corresponds to the standard fluorescence color-developing card (2), and the following steps are carried out one by one according to the following steps:
preparation of a polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a cuboid template (8) with a length of 8cm, a width of 4cm and a depth of 3mm of a resin material slowly, placing the polydimethylsiloxane solution in the cuboid template (8) for 10 minutes, placing a printed standard fluorescent color card (2) on the surface of the polydimethylsiloxane solution slowly, placing the polydimethylsiloxane solution for 10 minutes, placing the polydimethylsiloxane solution in a cuboid template (8) again, placing the cuboid template (8) in an oven with a temperature of 60-70 ℃, and taking out the cuboid template (8) after 3-5h to obtain a formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow filter paper into 6 wafers of 6mm by using a puncher, respectively soaking the obtained 6 wafers in 50, 100, 200, 300, 400 and 500 mu M coumarin-based probe molecule solutions one by one, naturally airing after 1 minute to obtain a paper-based sensing material (7) of 50-500 mu M coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. and c, respectively putting the paper-based sensing materials (7) of the 50-500 mu M coumarin-based probe molecules obtained in the step b into 6 reagent tanks (4) with the diameters of 6.5 and mm one by one, and obtaining the fluorescent sensing analysis card of the semi-quantitative potassium permanganate solution.
2. The application of the fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution obtained by the method according to claim 1 in the detection of potassium permanganate, adding 5-400 mu M potassium permanganate solution into a sample tank (6) with the diameter of 1cm, respectively introducing 5-400 mu M potassium permanganate solution into a reagent tank (4) with the diameter of 6.5mm through a drainage tank (5), waiting for the detection of the approximate concentration of potassium permanganate by the detection of the 5-400 mu M potassium permanganate solution after the potassium permanganate solution is fully contacted with a paper-based sensing material (7) loaded with coumarin-based probe molecules in the reagent tank (4) and irradiating by 365nm ultraviolet light, comparing fluorescence of the paper-based sensing material (7) of the coumarin-based probe molecules with fluorescence images after adding potassium permanganate with different concentrations in a standard fluorescent color card (2), and obtaining the approximate concentration of the potassium permanganate when the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard fluorescent color card (2).
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