CN113358619B - Preparation method and application of a fluorescent sensor analysis card for semi-quantitative detection of potassium permanganate solution - Google Patents

Abstract

The invention provides a preparation method and application of a fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution. The analysis card is composed of polydimethylsiloxane (PDMS) reaction substrate, standard fluorescent color display card and The reaction module is made of a reaction module, wherein the reaction module is an array composed of uniformly distributed reagent tanks and sample tank structures, the reagent tanks and sample tanks are connected through drainage grooves, and the reaction module is fixed at one end of the polydimethylsiloxane reaction base, and its The other end fixes the standard fluorescent color card, fills the reagent tank with paper-based sensing materials, and fills the test solution tank with the solution to be tested. The fluorescence change information on the reaction module corresponds to the standard fluorescent color card. When in use, Potassium permanganate solution was added to the sample tank, and the potassium permanganate solution was respectively put into the reagent tank through the drainage tank. After reacting for 20 s, it was irradiated with 365 nm ultraviolet light, and the fluorescence of the paper-based sensing material was compared with that of the standard fluorescence display. The concentration in the color card realizes the semi-quantitative detection of 5-400μM potassium permanganate solution. It overcomes the disadvantages of sequential dripping of reagents and poor detection timeliness, and improves the efficiency of detection and analysis.

Description

A method for preparing a fluorescent sensor analysis card for semi-quantitative detection of potassium permanganate solution law and application

technical field

The invention belongs to the field of detection and analysis of explosive residues, and in particular relates to a preparation method and application of a fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution.

Background technique

The main form of KMnO ₄ in the environment is liquid, and its excessive discharge will cause water pollution. It is very important to realize the detection of KMnO ₄ solution for environmental protection. At present, its detection methods include colorimetry with the help of optical microscope, flame atomic absorption method, etc., which not only need to use expensive and precise instruments, but also need complicated steps such as sample pretreatment and signal comparison analysis in the detection process, which is not conducive to on-site, Quick, visual identification of needs. If it is possible to integrate sampling, simultaneous contact with all detection reagents, fluorescence information changes, discoloration information, and result descriptions in one, with soft materials, small size and easy to carry, it will greatly improve the detection efficiency and accuracy of potassium permanganate residue analysis. big boost.

Contents of the invention

The purpose of the present invention is to provide a preparation method and application of a fluorescent sensor analysis card for semi-quantitative detection of potassium permanganate solution. The analysis card is made of polydimethylsiloxane (PDMS) reaction substrate, standard fluorescence It is made of a color card and a reaction module, wherein the reaction module is an array composed of evenly distributed reagent tanks and sample tanks. The reagent tanks and sample tanks are respectively connected by drainage grooves, and at one end of the polydimethylsiloxane reaction base The reaction module is fixed, the other end of which is fixed with a standard fluorescent color display card, the paper-based sensing material loaded with coumarin probes is filled in the reagent tank, the solution to be tested is filled in the sample tank, and the paper-based sensing material in the reagent tank is placed on the The fluorescence change information corresponds to the standard fluorescent color card one by one. When in use, add potassium permanganate solution into the liquid tank to be tested, and respectively enter the potassium permanganate solution into the reagent tank through the drainage tank. After reacting for 20 seconds, irradiate with 365nm ultraviolet light to compare the fluorescence of the paper-based sensing material with the The fluorescence image in the standard fluorescent color card can realize the semi-quantitative detection of 5-400μM potassium permanganate solution. It overcomes the disadvantages of sequential dripping of reagents and poor detection timeliness, and improves the efficiency of detection and analysis.

A method for preparing a fluorescent sensor analysis card for semi-quantitative detection of potassium permanganate solution according to the present invention, the analysis card uses a polydimethylsiloxane reaction substrate (1), a standard fluorescent color display card (2 ) and a reaction module (3), wherein the reaction module (3) is composed of 6 uniformly distributed reagent tanks (4) with a diameter of 6.5 mm and a depth of 2 mm and a sample tank (6) with a diameter of 1 cm and a depth of 1 mm. The composed array, the reagent tank (4) and the sample tank (6) are connected through the drainage tank (5), and the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction base (1), and the polydimethylsiloxane The other end of the oxane reaction base (1) is fixed with a standard fluorescent color card (2), and 6 reagent tanks (4) are filled with paper bases loaded with 50-500 μM coumarin-based probe molecules for detection of potassium permanganate The sensing material (7), the sample tank (6) is filled with the solution to be tested, and the fluorescence change information on the reaction module (3) corresponds to the standard fluorescent color display card (2) one by one. The specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template (8) with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card (2) into Place the surface of the dimethylsiloxane solution for 10 minutes, then pour 5ml of the polydimethylsiloxane solution into the cuboid template (8), and then put the cuboid template (8) into an oven at a temperature of 60-70°C. Take it out after 3-5 hours to obtain the formed polydimethylsiloxane reaction substrate (1);

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one After 1 minute, dry naturally to obtain a paper-based sensing material (7) of 50-500 μM coumarin-based probe molecules;

Prepare the fluorescence sensing assay card:

c. Put the paper-based sensing materials (7) of 50-500 μM coumarin-based probe molecules obtained in step b into six reagent tanks (4) with a diameter of 6.5 mm one by one to obtain semi-quantitative detection of permanganate Fluorescence sensing assay card for potassium solution.

Application of the fluorescent sensor analysis card for semi-quantitative detection of potassium permanganate solution obtained by the method in the preparation of potassium permanganate detection, adding 5-400 μM potassium permanganate solution in the sample tank (6) with a diameter of 1 cm , introduce 5-400 μ M potassium permanganate solution into the reagent tank (4) with a diameter of 6.5 mm through the drainage tank (5) respectively, and wait for the potassium permanganate solution and the coumarin base in the reagent tank (4) After fully contacting the paper-based sensing material (7) of the probe molecule for 20 seconds, irradiate with 365nm ultraviolet light to compare the fluorescence of the paper-based sensing material (7) of the coumarin-based probe molecule with the standard fluorescent color card (2) Fluorescent images of different concentrations of potassium permanganate added in, when the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard color card (2), the approximate concentration of potassium permanganate can be obtained, and the 5-400μM potassium permanganate solution semi-quantitative detection.

A preparation method and application of a fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution according to the present invention, the fluorescent sensing analysis card obtained by the method is effective in detecting 5-400 μM potassium permanganate solution step:

Add the liquid potassium permanganate solution to be tested into the sample tank (6) with a diameter of 1 cm in the reaction module (3) fixed at one end of the polydimethylsiloxane reaction substrate (1), and the potassium permanganate solution to be tested is respectively along the Follow the drainage groove (5) with an inclination angle to flow into the six connected reagent tanks (4) with built-in paper-based sensing materials (7), and then ensure that the liquid potassium permanganate solution to be tested is evenly distributed in the reagent tank ( In 4), after the potassium permanganate solution is in full contact with the paper-based sensing material (7) in the reagent tank (4) for 20 seconds, a 365nm ultraviolet lamp is used to irradiate the reaction area to react with polydimethylsiloxane The fluorescent color information of the standard fluorescent color display card (2) fixed at the other end of the substrate (1) is checked and compared, so as to clarify the content range of the potassium permanganate solution in the object to be tested. It can realize the functions of fast semi-quantitative identification and integrated operation comparison. The fluorescent sensing analysis card of the present invention is soft in material, stable in physical and chemical properties, small in size and easy to carry, greatly reduces operating time and detection time, and improves detection and analysis efficiency and accuracy of test results. It overcomes the disadvantages of sequentially dropping samples of the analyte or requiring mixed use of detection reagents, performing sequence analysis, and poor detection timeliness.

Compared with the prior art, the present invention has the following beneficial effects:

(1) An array composed of 6 reagent tanks and 1 sample tank structure that can place different concentrations of paper-based sensing materials is constructed, which can realize semi-quantitative detection by adding samples at one time, overcoming the shortcomings of sequential dripping of reagents and poor detection timeliness .

(2) The standard fluorescence color display card built in the fluorescence sensor analysis card of the present invention mainly contains fluorescence color change information, which can truly realize the integration of sample addition, fluorescence color development, and identification, and improve the efficiency of detection and analysis.

(3) The material of the fluorescent sensor analysis card of the present invention is mainly PDMS, and its curvature can reach 180°, and its hydrophobic and stable physical and chemical properties are not easily affected by changes in environmental temperature and humidity, and it also brings great inconvenience to carrying. convenient.

(4) The detection time does not exceed 20 seconds, and the detection and identification process is quickly completed.

Description of drawings

Fig. 1 is a structural representation of the present invention;

Fig. 2 is a schematic diagram of a template used for forming a polydimethylsiloxane (PDMS) substrate of the present invention.

Detailed ways

Further description below in conjunction with accompanying drawings.

Example 1

A preparation method and application of a fluorescence sensing analysis card for semi-quantitative detection of potassium permanganate solution according to the present invention, the analysis card uses a polydimethylsiloxane reaction substrate 1, a standard fluorescent color display card 2 and The reaction module 3 is composed of a reaction module 3, wherein the reaction module 3 is an array composed of 6 uniformly distributed reagent tanks 4 with a diameter of 6.5 mm and a depth of 2 mm and a sample tank 6 with a diameter of 1 cm and a depth of 1 mm. 6 is connected through the drainage groove 5, the reaction module 3 is fixed at one end of the polydimethylsiloxane reaction substrate 1, and the standard fluorescent color display card 2 is fixed at the other end of the polydimethylsiloxane reaction substrate 1, and the six reagents Tank 4 is filled with paper-based sensing material 7 loaded with 50-500 μM coumarin-based probe molecules for detecting potassium permanganate, and sample tank 6 is filled with the solution to be tested. The fluorescence change information on the reaction module 3 is consistent with the standard fluorescence display. The color card 2 corresponds one by one, and the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane On the surface of the oxane solution, let it stand for 10 minutes, then take 5ml of polydimethylsiloxane solution and pour it into the cuboid template 8, then put the cuboid template 8 into an oven at a temperature of 60°C, take it out after 5 hours, and obtain the formed polydimethylsiloxane Methylsiloxane Reactive Substrate 1;

Preparation of paper-based sensing material:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one , and dried naturally after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules at 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, a 5 μM potassium permanganate solution was added to the sample groove 6 with a diameter of 1 cm, and through the drainage Tank 5 put 5 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 5 μM potassium permanganate solution to be tested and 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin in reagent tank 4 After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. The fluorescence image after adding potassium manganate, the fluorescence change in the paper-based sensing material 7 is close to the fluorescence change of 10 μM KMnO ₄ in the standard color rendering card 2, that is, it is similar to the results in the first row of the standard fluorescence color card 2, that is, a high The approximate concentration of potassium manganate is less than 10 μM.

Example 2

The composition of the fluorescent sensor analysis card is carried out according to embodiment 1: the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane On the surface of the oxane solution, let it stand for 10 minutes, then pour 5ml of polydimethylsiloxane solution into the cuboid formwork 8, then put the cuboid formwork 8 into an oven at a temperature of 70°C, take it out after 3 hours, and obtain the formed polydimethylsiloxane Methylsiloxane Reactive Substrate 1;

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one , and dried naturally after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules at 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, 80 μM potassium permanganate solution was added to the sample groove 6 with a diameter of 1 cm, and the drainage Tank 5 puts 80 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 80 μM potassium permanganate solution to be tested is mixed with 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin in reagent tank 4 After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. Fluorescence image after adding potassium manganate, the fluorescence change in the paper-based sensing material 7 shows gradually enhanced blue fluorescence _. The approximate concentration of potassium phosphate is 50-100 μM.

Example 3

The composition of the fluorescent sensor analysis card is carried out according to embodiment 1: the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane On the surface of the oxane solution, let it stand for 10 minutes, then take 5ml of polydimethylsiloxane solution and pour it into the cuboid template 8, then put the cuboid template 8 into an oven at a temperature of 65°C, take it out after 4 hours, and obtain the formed polydimethylsiloxane Methylsiloxane Reactive Substrate 1;

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one , and dried naturally after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules at 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, a 100 μM potassium permanganate solution was added to the sample groove 6 with a diameter of 1 cm, and through the drainage Tank 5 puts 100 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 100 μM potassium permanganate solution and reagent tank 4 contain 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. In the fluorescence image after the addition of potassium manganate, the fluorescence change in the paper-based sensing material 7 shows an obvious blue fluorescence enhancement trend, which corresponds to the fluorescence change of 100 μM KMnO ₄ in the standard color rendering card 2, that is, the approximate concentration of potassium permanganate 100 μM.

Example 4

The composition of the fluorescent sensor analysis card is carried out according to embodiment 1: the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane On the surface of the oxane solution, let it stand for 10 minutes, then take 5ml of polydimethylsiloxane solution and pour it into the cuboid template 8, then put the cuboid template 8 into an oven at a temperature of 60°C, take it out after 5 hours, and obtain the formed polydimethylsiloxane Methylsiloxane reactive substrate (1);

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one , and dried naturally after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules at 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, a 160 μM potassium permanganate solution was added to the sample groove 6 with a diameter of 1 cm, and through the drainage Tank 5 puts 160 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 160 μM potassium permanganate solution to be tested is mixed with 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin in reagent tank 4 After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. The fluorescence image after adding potassium manganate, the fluorescence change in the paper-based sensing material 7 and the stronger blue fluorescence that gradually increases, are between the fluorescence changes of 100 and 200 μM KMnO ₄ in the standard color rendering card 2, that is, The approximate concentration of potassium permanganate is 100-200 μM.

Example 5

The composition of the fluorescent sensor analysis card is carried out according to embodiment 1: the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane Put it on the surface of the oxane solution for 10 minutes, then pour 5ml of polydimethylsiloxane solution into the cuboid template 8, then put the cuboid template 8 into an oven at a temperature of 63°C, take it out after 5 hours, and obtain the formed polydimethylsiloxane Methylsiloxane Reactive Substrate 1;

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6 discs of 6 mm with a puncher, and soak the obtained 6 discs in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions one by one , and dried naturally after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules at 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, a 266 μM potassium permanganate solution was added to the sample groove 6 with a diameter of 1 cm, and through the drainage Tank 5 puts 266 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 266 μM potassium permanganate solution to be tested is mixed with 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin in reagent tank 4 After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. The fluorescence image after adding potassium manganate, the fluorescence change in the paper-based sensing material 7 shows a gradually enhanced strong blue fluorescence, the fluorescence change in the paper-based sensing material 7 is between 200 μM and 300 μM KMnO in the standard color rendering card 2 Between ₄ changes in fluorescence, the approximate concentration of potassium permanganate obtained is 200-300 μM.

Example 6

The composition of the fluorescent sensor analysis card is carried out according to embodiment 1: the specific operation is carried out according to the following steps:

Prepare the polydimethylsiloxane reaction substrate:

a. Put polydimethylsiloxane and peroxide vulcanizing agent in a beaker at a volume ratio of 10:1 and stir until they dissolve evenly to obtain a polydimethylsiloxane solution. After standing for 30 minutes, take 5ml of polydimethylsiloxane The methyl siloxane solution is slowly poured into a rectangular parallelepiped template 8 of resin material with a length of 8 cm, a width of 4 cm, and a depth of 3 mm. After standing for 10 minutes, slowly put the printed standard fluorescent color card 2 into polydimethylsiloxane On the surface of the oxane solution, let it stand for 10 minutes, then take 5ml of polydimethylsiloxane solution and pour it into the cuboid template 8, then put the cuboid template 8 into an oven at a temperature of 70°C, take it out after 5 hours, and obtain the formed polydimethylsiloxane Methylsiloxane Reactive Substrate 1;

Preparation of paper-based sensing materials:

b. Cut the commercial slow filter paper into 6mm discs with a puncher, soak the obtained discs one by one in 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule solutions for 1 minute After drying naturally, 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM paper-based sensing materials 7 loaded with coumarin-based probe molecules were obtained;

Prepare the fluorescence sensing assay card:

c. Put the 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecule paper-based sensing materials 7 obtained in step b into six reagent tanks 4 with a diameter of 6.5 mm one by one to obtain semi-quantitative detection Fluorescent sensor analysis card for potassium permanganate solution;

In use, according to the paper-based sensing material 7 of 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin-based probe molecules, a 400 μM potassium permanganate solution was added into the sample groove 6 with a diameter of 1 cm, and through the drainage Tank 5 put 400 μM potassium permanganate solution into reagent tank 4 with a diameter of 6.5 mm, and the 400 μM potassium permanganate solution to be tested and 50 μM, 100 μM, 200 μM, 300 μM, 400 μM and 500 μM coumarin in reagent tank 4 After the paper-based sensing material 7 based on the probe molecule is fully exposed for 20 seconds, it is irradiated with 365nm ultraviolet light, and the fluorescence of the paper-based sensing material 7 based on the probe molecule is compared with that in the standard fluorescent color card 2. Fluorescence image after adding potassium manganate, the fluorescence change in the paper-based sensing material 7 shows a gradually enhanced strong blue fluorescence, which is close to the fluorescence change of 400 μM KMnO ₄ in the standard color rendering card 2, that is, potassium permanganate The approximate concentration is 400 μM.

The above are the preferred embodiments of the present invention, and all changes made according to the technical solution of the present invention, when the functional effect produced does not exceed the scope of the technical solution of the present invention, all belong to the protection scope of the present invention.

Claims (2)

1. The preparation method of the fluorescence sensing analysis card for semi-quantitatively detecting the potassium permanganate solution is characterized in that the analysis card consists of a polydimethylsiloxane reaction substrate (1), a standard fluorescence color-developing card (2) and a reaction module (3), wherein the reaction module (3) is an array formed by uniformly distributing 6 reagent tanks (4) with the diameters of 6.5mm and the depths of 2mm and sample tanks (6) with the diameters of 1cm and the depths of 1mm, the reagent tanks (4) and the sample tanks (6) are connected through a drainage tank (5), the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction substrate (1), the standard fluorescence color-developing card (2) is fixed at the other end of the polydimethylsiloxane reaction substrate (1), paper-based sensing materials (7) loaded with 50-500 mu M coumarin-based probe molecules of potassium permanganate are respectively filled in the 6 reagent tanks (4), the sample tanks (6) are filled with to-be-detected solution, fluorescence change information on the reaction module (3) corresponds to the standard fluorescence color-developing card (2), and the following steps are carried out one by one according to the following steps:

preparation of a polydimethylsiloxane reaction substrate:

a. placing polydimethylsiloxane and peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing the polydimethylsiloxane solution in a cuboid template (8) with a length of 8cm, a width of 4cm and a depth of 3mm of a resin material slowly, placing the polydimethylsiloxane solution in the cuboid template (8) for 10 minutes, placing a printed standard fluorescent color card (2) on the surface of the polydimethylsiloxane solution slowly, placing the polydimethylsiloxane solution for 10 minutes, placing the polydimethylsiloxane solution in a cuboid template (8) again, placing the cuboid template (8) in an oven with a temperature of 60-70 ℃, and taking out the cuboid template (8) after 3-5h to obtain a formed polydimethylsiloxane reaction substrate (1);

preparing a paper-based sensing material:

b. cutting commercial slow filter paper into 6 wafers of 6mm by using a puncher, respectively soaking the obtained 6 wafers in 50, 100, 200, 300, 400 and 500 mu M coumarin-based probe molecule solutions one by one, naturally airing after 1 minute to obtain a paper-based sensing material (7) of 50-500 mu M coumarin-based probe molecules;

preparing a fluorescence sensing analysis card:

c. and c, respectively putting the paper-based sensing materials (7) of the 50-500 mu M coumarin-based probe molecules obtained in the step b into 6 reagent tanks (4) with the diameters of 6.5 and mm one by one, and obtaining the fluorescent sensing analysis card of the semi-quantitative potassium permanganate solution.

2. The application of the fluorescence sensing analysis card of the semi-quantitative potassium permanganate solution obtained by the method according to claim 1 in the detection of potassium permanganate, adding 5-400 mu M potassium permanganate solution into a sample tank (6) with the diameter of 1cm, respectively introducing 5-400 mu M potassium permanganate solution into a reagent tank (4) with the diameter of 6.5mm through a drainage tank (5), waiting for the detection of the approximate concentration of potassium permanganate by the detection of the 5-400 mu M potassium permanganate solution after the potassium permanganate solution is fully contacted with a paper-based sensing material (7) loaded with coumarin-based probe molecules in the reagent tank (4) and irradiating by 365nm ultraviolet light, comparing fluorescence of the paper-based sensing material (7) of the coumarin-based probe molecules with fluorescence images after adding potassium permanganate with different concentrations in a standard fluorescent color card (2), and obtaining the approximate concentration of the potassium permanganate when the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard fluorescent color card (2).

Images