CN113358619A - Preparation method and application of fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution - Google Patents
Preparation method and application of fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution Download PDFInfo
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Abstract
The invention provides a method for preparing a fluorescent sensing analysis card for semi-quantitatively detecting potassium permanganate solution and application thereof, the analysis card is prepared by a Polydimethylsiloxane (PDMS) reaction substrate, a standard fluorescent color development card and a reaction module, wherein the reaction module is an array composed of uniformly distributed reagent grooves and sample groove structures, the reagent grooves and the sample grooves are connected through drainage grooves, one end of the PDMS reaction substrate is fixed with the reaction module, the other end of the PDMS reaction substrate is fixed with the standard fluorescent color development card, the reagent grooves are filled with paper-based sensing materials, the liquid grooves to be detected are filled with the solution to be detected, the fluorescent change information on the reaction modules corresponds to the standard fluorescent color development cards one to one, when in use, the potassium permanganate solution is added into the sample grooves through the drainage grooves, the potassium permanganate solution respectively enters the reagent grooves through the drainage grooves, after 20s of reaction, the potassium permanganate solution is irradiated by 365nm ultraviolet light, and comparing the concentration of the paper-based sensing material fluorescence with that of a standard fluorescence color development card to realize semi-quantitative detection of 5-400 mu M potassium permanganate solution. The defects of reagent dripping in sequence and poor detection timeliness are overcome, and the detection and analysis efficiency is improved.
Description
Technical Field
The invention belongs to the field of explosive residue detection and analysis, and particularly relates to a preparation method and application of a fluorescent sensing analysis card for semi-quantitatively detecting a potassium permanganate solution.
Background
KMnO4The main existing form in the environment is liquid, excessive discharge of the liquid can cause water body pollution, and KMnO is realized4Solution detection is very important for environmental protection. At present, the detection method comprises a colorimetric method, a flame atomic absorption method and the like by means of an optical microscope, expensive and precise instruments are required, complicated sample pretreatment, signal comparison analysis and other steps are required in the detection process, and the detection method is not favorable for on-site, rapid and visual identification requirements. If can with sampling, with all detect reagent simultaneous contact, fluorescence information change, information and the result description collection in an organic whole and material soft, small in size easily carry, will bring very big promotion to potassium permanganate residual analysis's detection efficiency and accuracy.
Disclosure of Invention
The invention aims to provide a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, wherein the analysis card is prepared from a Polydimethylsiloxane (PDMS) reaction substrate, a standard fluorescence color development card and a reaction module, the reaction module is an array formed by uniformly distributed reagent grooves and sample groove structures, the reagent grooves and the sample grooves are respectively connected through drainage grooves, the reaction module is fixed at one end of the PDMS reaction substrate, the standard fluorescence color development card is fixed at the other end of the PDMS reaction substrate, a paper-based sensing material loaded with coumarin probes is filled in the reagent grooves, a solution to be detected is filled in the sample grooves, and fluorescence change information on the paper-based sensing material in the reagent grooves corresponds to the standard fluorescence color development card one to one. When the detection kit is used, a potassium permanganate solution is added into a liquid groove to be detected, the potassium permanganate solution enters a reagent groove through a drainage groove respectively, after the reaction is carried out for 20s, the fluorescence of the paper-based sensing material is compared with a fluorescence image in a standard fluorescence color development card through 365nm ultraviolet irradiation, and the semi-quantitative detection of the 5-400 mu M potassium permanganate solution is realized. The defects of reagent dripping in sequence and poor detection timeliness are overcome, and the detection and analysis efficiency is improved.
The invention relates to a preparation method of a fluorescence sensing analysis card for semi-quantitatively detecting potassium permanganate solution, which comprises a polydimethylsiloxane reaction substrate (1), a standard fluorescence color development card (2) and a reaction module (3), wherein the reaction module (3) is an array consisting of 6 uniformly distributed reagent grooves (4) with the diameter of 6.5mm and the depth of 2mm and a sample groove (6) with the diameter of 1cm and the depth of 1mm, the reagent grooves (4) and the sample groove (6) are connected through a drainage groove (5), the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction substrate (1), the standard fluorescence color development card (2) is fixed at the other end of the polydimethylsiloxane reaction substrate (1), a paper-based sensing material (7) loaded with 50-500 mu M coumarin-based probe molecules for detecting potassium permanganate is filled in the 6 reagent grooves (4), the sample groove (6) is filled with a solution to be detected, the fluorescence change information on the reaction module (3) corresponds to the standard fluorescence color development cards (2) one by one, and the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to the volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, slowly pouring 5ml of the polydimethylsiloxane solution into a cuboid template (8) made of resin with the length of 8cm, the width of 4cm and the depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card (2) on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template (8), placing the cuboid template (8) into a baking oven with the temperature of 60-70 ℃, and taking out after 3-5 hours to obtain a formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing after 1 minute to obtain a paper-based sensing material (7) with the diameters of 50-500 mu M coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. and c, respectively putting the paper-based sensing materials (7) of the 50-500 mu M coumarin-based probe molecules obtained in the step b into 6 reagent grooves (4) with the diameter of 6.5mm one by one to obtain the fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution.
The fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution obtained by the method is applied to the detection of preparing the potassium permanganate, 5-400 mu M of the potassium permanganate solution is added into a sample groove (6) with the diameter of 1cm, the 5-400 mu M of the potassium permanganate solution is respectively introduced into a reagent groove (4) with the diameter of 6.5mm through a drainage groove (5), after the potassium permanganate solution of a detected object is fully contacted with a paper-based sensing material (7) of coumarin-based probe molecules in the reagent groove (4) for 20s, the fluorescence image of the added paper-based sensing material (7) of the coumarin-based probe molecules and potassium permanganate with different concentrations in a standard fluorescent color development card (2) is compared through 365nm ultraviolet irradiation, when the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard color development card (2), the approximate concentration of the potassium permanganate is obtained, namely, the semi-quantitative detection of the 5-400 mu M potassium permanganate solution is realized.
The invention relates to a preparation method and application of a fluorescence sensing analysis card for semi-quantitatively detecting a potassium permanganate solution, wherein the fluorescence sensing analysis card obtained by the method comprises the following steps of:
adding a liquid potassium permanganate solution to be detected into a sample groove (6) with the diameter of 1cm in a reaction module (3) fixed at one end of a polydimethylsiloxane reaction substrate (1), enabling the liquid potassium permanganate solution to be detected to flow into 6 reagent grooves (4) with paper-based sensing materials (7) arranged in the reagent grooves (4) respectively along drainage grooves (5) with inclination angles, then ensuring that the liquid potassium permanganate solution to be detected is uniformly distributed in the reagent grooves (4), after the liquid potassium permanganate solution to be detected is in full contact with the paper-based sensing materials (7) in the reagent grooves (4) for 20s, irradiating a reaction area by using a 365nm ultraviolet lamp, and performing calibration comparison with fluorescence color information of a standard fluorescence color development card (2) fixed at the other end of the polydimethylsiloxane reaction substrate (1), thereby determining the content range of the potassium permanganate solution in the object to be detected. The functions of quick semi-quantitative identification and operation and comparison integration can be realized. The fluorescence sensing analysis card is soft in material, stable in physical and chemical properties, small in size and easy to carry, operation time and detection time are greatly shortened, and detection analysis efficiency and accuracy of a test result are improved. The defects that the sample to be detected is sequentially dripped or the detection reagent needs to be mixed for use, the sequence analysis is executed and the detection timeliness is poor are overcome.
Compared with the prior art, the invention has the following beneficial effects:
(1) the array composed of 6 reagent grooves capable of containing paper-based sensing materials with different concentrations and 1 sample groove structure is constructed, semi-quantitative detection can be realized by one-time sample adding, and the defects of reagent dripping in sequence and poor detection timeliness are overcome.
(2) The standard fluorescent color development card arranged in the fluorescent sensing analysis card mainly contains fluorescent color change information, can really realize integration of sample adding, fluorescent color development and identification, and improves the detection and analysis efficiency.
(3) The fluorescence sensing analysis card is mainly made of PDMS, the flexibility of the fluorescence sensing analysis card can reach 180 degrees, the hydrophobic and stable physical and chemical properties are not easily influenced by the change of the temperature and the humidity of the environment, and great convenience is brought to carrying.
(4) The detection time is not more than 20 seconds, and the detection and identification process is completed quickly.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of a template for forming a Polydimethylsiloxane (PDMS) substrate according to the present invention.
Detailed Description
This is further explained below with reference to the drawings.
Example 1
The invention relates to a method for preparing a fluorescent sensing analysis card for semi-quantitatively detecting potassium permanganate solution and application, the analysis card consists of a polydimethylsiloxane reaction substrate 1, a standard fluorescent color development card 2 and a reaction module 3, wherein the reaction module 3 is an array consisting of 6 uniformly distributed reagent grooves 4 with the diameter of 6.5mm and the depth of 2mm and sample grooves 6 with the diameter of 1cm and the depth of 1mm, the reagent grooves 4 and the sample grooves 6 are connected by drainage grooves 5, the reaction module 3 is fixed at one end of the polydimethylsiloxane reaction substrate 1, the standard fluorescent color development card 2 is fixed at the other end of the polydimethylsiloxane reaction substrate 1, paper-based sensing materials 7 which are loaded with 50-500 mu M coumarin-based probe molecules for detecting potassium permanganate are filled in the 6 reagent grooves 4, a solution to be detected is filled in the sample grooves 6, fluorescent change information on the reaction module 3 corresponds to the standard fluorescent color development card 2 one by one, the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, taking 5ml of the polydimethylsiloxane solution, slowly pouring the polydimethylsiloxane solution into a cuboid template 8 with a resin material length of 8cm, a width of 4cm and a depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into a drying oven with a temperature of 60 ℃, and taking out after 5 hours to obtain a formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the wafers after 1 minute to obtain paper-based sensing materials 7 with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M loaded with coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, the paper-based sensing material 7 according to 50. mu.M, 100. mu.M, 200. mu.M, 300. mu.M, 400. mu.M and 500. mu.M coumarin-based probe molecules, at a diameter of 1cAdding 5 mu M potassium permanganate solution into a sample groove 6, respectively entering 5 mu M potassium permanganate solution into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, waiting for the 5 mu M potassium permanganate solution of a test object to fully contact 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M coumarin-based probe molecule paper-based sensing material 7 in the reagent groove 4 for 20s, irradiating by 365nm ultraviolet light, comparing the fluorescence of the paper-based sensing material 7 of the coumarin-based probe molecule with the fluorescence image of the added potassium permanganate with different concentrations in a standard fluorescence color development card 2, and comparing the fluorescence change in the paper-based sensing material 7 with the 10 mu M KMnO in the standard color development card 24The fluorescence change of the standard fluorescent colorimetric card is approximate, namely the result is similar to the first row result of the standard fluorescent colorimetric card 2, and the concentration of the obtained potassium permanganate is approximately less than 10 mu M.
Example 2
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, slowly pouring 5ml of the polydimethylsiloxane solution into a cuboid template 8 with a resin material length of 8cm, a width of 4cm and a depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into a drying oven with a temperature of 70 ℃, and taking out after 3 hours to obtain a formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the wafers after 1 minute to obtain paper-based sensing materials 7 with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M loaded with coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, according to the paper-based sensing material 7 of coumarin-based probe molecules with the diameter of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M, 80 mu M of potassium permanganate solution is added into a sample groove 6 with the diameter of 1cm, the 80 mu M of potassium permanganate solution is respectively put into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, after the 80 mu M of potassium permanganate solution of an object to be detected is fully contacted with the paper-based sensing material 7 of coumarin-based probe molecules with the diameter of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M in the reagent groove 4 for 20s, the fluorescence change and the gradually enhanced blue fluorescence are presented in the paper-based sensing material 7 by 365nm ultraviolet irradiation to compare the fluorescence of the paper-based sensing material 7 of the coumarin-based probe molecules with the fluorescence image of potassium permanganate added in the standard fluorescent color development card 2 with different concentrations, and the fluorescence change and the gradually enhanced blue fluorescence are presented in the paper-based sensing material 7, wherein the fluorescence of the fluorescent color development card 2 is between 50, the fluorescent color development card, 100 μ M KMnO4The fluorescent change of the fluorescent material can obtain the potassium permanganate with approximate concentration of 50-100 mu M.
Example 3
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, slowly pouring 5ml of the polydimethylsiloxane solution into a cuboid template 8 with a resin material length of 8cm, a width of 4cm and a depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into an oven with a temperature of 65 ℃, and taking out after 4 hours to obtain a formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the wafers after 1 minute to obtain paper-based sensing materials 7 with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M loaded with coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, paper-based sensing material 7 based on 50. mu.M, 100. mu.M, 200. mu.M, 300. mu.M, 400. mu.M and 500. mu.M coumarin-based probe molecules, adding 100 mu M potassium permanganate solution into a sample groove 6 with the diameter of 1cm, respectively entering the 100 mu M potassium permanganate solution into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, after 100 mu M potassium permanganate solution of an object to be detected is fully contacted with paper-based sensing materials 7 of coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M in a reagent tank 4 for 20s, the paper-based sensing materials are irradiated by 365nm ultraviolet light, comparing the fluorescence of the paper-based sensing material 7 of the coumarin-based probe molecule with the fluorescence image of the added potassium permanganate with different concentrations in the standard fluorescence color development card 2, the fluorescence change in the paper-based sensing material 7 shows a more obvious blue fluorescence enhancement trend, and the blue fluorescence enhancement trend is compared with that of 100 mu MKMnO in the standard color development card 2.4The fluorescent change of the potassium permanganate is corresponding to the fluorescent change of the potassium permanganate, and the concentration of the potassium permanganate is about 100 mu M.
Example 4
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to the volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, taking 5ml of the polydimethylsiloxane solution, slowly pouring the polydimethylsiloxane solution into a cuboid template 8 with the resin material length of 8cm, the width of 4cm and the depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into a drying oven with the temperature of 60 ℃, and taking out after 5 hours to obtain a formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the wafers after 1 minute to obtain paper-based sensing materials 7 with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M loaded with coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, according to paper-based sensing materials 7 of coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M, 160 mu M of potassium permanganate solution is added into a sample groove 6 with the diameter of 1cm, 160 mu M of potassium permanganate solution is respectively added into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, after the 160 mu M of potassium permanganate solution of an object to be detected is fully contacted with the paper-based sensing materials 7 of coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M in the reagent groove 4 for 20s, the paper-based sensing materials 7 are irradiated by 365nm ultraviolet light, and compared with fluorescence images of the paper-based sensing materials 7 of the coumarin-based probe molecules and fluorescence images of potassium permanganate added in different concentrations in a standard fluorescence color development card 2, the fluorescence change in the paper-based sensing materials 7 is stronger and gradually enhanced blue fluorescence is presented, and the fluorescence is between 100, 200 mu M, 300 mu M, 400 mu M and 500 mu M in the standard color development card 2, 200 μ M KMnO4The fluorescence change of the fluorescent material is that the potassium permanganate is approximately 100-200 mu M.
Example 5
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, slowly pouring 5ml of the polydimethylsiloxane solution into a cuboid template 8 with a resin material length of 8cm, a width of 4cm and a depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into a drying oven with a temperature of 63 ℃, and taking out after 5 hours to obtain a formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into coumarin-based probe molecule solutions with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the wafers after 1 minute to obtain paper-based sensing materials 7 with the diameters of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M loaded with coumarin-based probe molecules;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, according to paper-based sensing materials 7 of coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M, a 266 mu M potassium permanganate solution is added into a sample groove 6 with the diameter of 1cm, the 266 mu M potassium permanganate solution is respectively put into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, and paper-based transmission of the 266 mu M potassium permanganate solution of an object to be detected and the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M in the reagent groove 4 is waitedAfter the sensing material 7 is fully contacted for 20s, through 365nm ultraviolet irradiation, comparing the fluorescence of the paper-based sensing material 7 of the coumarin-based probe molecule with the fluorescence image of the standard fluorescent color development card 2 added with potassium permanganate with different concentrations, the fluorescence change in the paper-based sensing material 7 and the gradually enhanced strong blue fluorescence are presented, and the fluorescence change in the paper-based sensing material 7 is between 200 mu M and 300 mu M KMnO in the standard color development card 24The fluorescence change of the fluorescent material is that the potassium permanganate is approximately 200-300 mu M.
Example 6
The composition of the fluorescence sensor assay card was performed according to example 1: the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to a volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, taking 5ml of the polydimethylsiloxane solution, slowly pouring the polydimethylsiloxane solution into a cuboid template 8 with a resin material length of 8cm, a width of 4cm and a depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card 2 on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template 8, placing the cuboid template 8 into a drying oven with a temperature of 70 ℃, and taking out after 5 hours to obtain a formed polydimethylsiloxane reaction substrate 1;
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6mm round pieces by using a puncher, respectively soaking the round pieces into coumarin-based probe molecule solutions of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M one by one, and naturally airing the round pieces after 1 minute to obtain paper-based sensing materials 7 loaded with coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M;
preparing a fluorescence sensing analysis card:
c. putting the paper-based sensing materials 7 of the coumarin-based probe molecules of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M obtained in the step b into 6 reagent grooves 4 with the diameter of 6.5mm one by one to obtain a fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution;
in use, paper-based sensing material 7 based on 50. mu.M, 100. mu.M, 200. mu.M, 300. mu.M, 400. mu.M and 500. mu.M coumarin-based probe molecules, adding 400 mu M potassium permanganate solution into a sample groove 6 with the diameter of 1cm, respectively entering the 400 mu M potassium permanganate solution into a reagent groove 4 with the diameter of 6.5mm through a drainage groove 5, after the paper-based sensing material 7 of the coumarin-based probe molecules with the concentration of the potassium permanganate solution of 400 mu M to be detected and the concentration of the coumarin-based sensing material of 50 mu M, 100 mu M, 200 mu M, 300 mu M, 400 mu M and 500 mu M in the reagent tank 4 are fully contacted for 20s, the paper-based sensing material is irradiated by 365nm ultraviolet light, comparing the fluorescence of the paper-based sensing material 7 of the coumarin-based probe molecule with the fluorescence image of the added potassium permanganate with different concentrations in the standard fluorescence color development card 2, the fluorescence change and the gradually enhanced strong blue fluorescence in the paper-based sensing material 7 are compared with 400 mu M KMnO in the standard color development card 2.4The fluorescence change is close to obtain the potassium permanganate with the approximate concentration of 400 mu M.
The above are preferred embodiments of the present invention, and all changes made according to the technical scheme of the present invention that produce functional effects do not exceed the scope of the technical scheme of the present invention belong to the protection scope of the present invention.
Claims (2)
1. A method for preparing a fluorescence sensing analysis card for semi-quantitatively detecting a potassium permanganate solution is characterized in that the analysis card consists of a polydimethylsiloxane reaction substrate (1), a standard fluorescence color development card (2) and a reaction module (3), wherein the reaction module (3) is an array which is composed of 6 uniformly distributed reagent grooves (4) with the diameter of 6.5mm and the depth of 2mm and a sample groove (6) with the diameter of 1cm and the depth of 1mm, the reagent grooves (4) and the sample groove (6) are connected through a drainage groove (5), the reaction module (3) is fixed at one end of the polydimethylsiloxane reaction substrate (1), the standard fluorescence color development card (2) is fixed at the other end of the polydimethylsiloxane reaction substrate (1), paper-based sensing materials (7) loaded with 50-500 mu M coumarin-based probe molecules for detecting potassium permanganate are respectively filled in the 6 reagent grooves (4), the sample groove (6) is filled with a solution to be detected, the fluorescence change information on the reaction module (3) corresponds to the standard fluorescence color development cards (2) one by one, and the specific operation is carried out according to the following steps:
preparation of polydimethylsiloxane reaction substrate:
a. placing polydimethylsiloxane and a peroxide vulcanizing agent in a beaker according to the volume ratio of 10:1, stirring until the polydimethylsiloxane and the peroxide vulcanizing agent are uniformly dissolved to obtain a polydimethylsiloxane solution, placing for 30 minutes, slowly pouring 5ml of the polydimethylsiloxane solution into a cuboid template (8) made of a resin material and having the length of 8cm, the width of 4cm and the depth of 3mm, placing for 10 minutes, slowly placing a printed standard fluorescent color development card (2) on the surface of the polydimethylsiloxane solution, placing for 10 minutes, pouring 5ml of the polydimethylsiloxane solution into the cuboid template (8), placing the cuboid template (8) into a baking oven at the temperature of 60-70 ℃, and taking out after 3-5 hours to obtain a formed polydimethylsiloxane reaction substrate (1);
preparing a paper-based sensing material:
b. cutting commercial slow-speed filter paper into 6 wafers with the diameter of 6mm by using a puncher, respectively soaking the 6 wafers into 50, 100, 200, 300, 400 and 500 mu M coumarin-based probe molecule solutions one by one, and naturally airing the wafers after 1 minute to obtain a paper-based sensing material (7) with the coumarin-based probe molecule concentration of 50-500 mu M;
preparing a fluorescence sensing analysis card:
c. and c, respectively putting the paper-based sensing materials (7) of the 50-500 mu M coumarin-based probe molecules obtained in the step b into 6 reagent grooves (4) with the diameter of 6.5mm one by one to obtain the fluorescent sensing analysis card for semi-quantitatively detecting the potassium permanganate solution.
2. The application of the fluorescent sensing analysis card for semi-quantitative detection of potassium permanganate solution obtained according to the method of claim 1 in the detection of potassium permanganate preparation, wherein 5-400 μ M potassium permanganate solution is added into a sample groove (6) with the diameter of 1cm, 5-400 μ M potassium permanganate solution is respectively introduced into a reagent groove (4) with the diameter of 6.5mm through a drainage groove (5), after the potassium permanganate solution to be detected and the paper-based sensing material (7) loaded with coumarin-based probe molecules in the reagent groove (4) are fully contacted for 20s, fluorescent images after the fluorescence of the paper-based sensing material (7) loaded with coumarin-based probe molecules and the potassium permanganate added with different concentrations in a standard fluorescent color development card (2) are compared through 365nm ultraviolet irradiation, when the fluorescence change in the paper-based sensing material (7) corresponds to the fluorescence change in the standard color development card (2), the approximate concentration of the potassium permanganate is obtained, and the semi-quantitative detection of the 5-400 mu M potassium permanganate solution is realized.
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