CN114921386A - Sewage treatment microbial inoculum and preparation method thereof - Google Patents

Sewage treatment microbial inoculum and preparation method thereof Download PDF

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Publication number
CN114921386A
CN114921386A CN202210723060.0A CN202210723060A CN114921386A CN 114921386 A CN114921386 A CN 114921386A CN 202210723060 A CN202210723060 A CN 202210723060A CN 114921386 A CN114921386 A CN 114921386A
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China
Prior art keywords
microbial inoculum
sewage treatment
sodium alginate
treatment microbial
zeolite
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CN202210723060.0A
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Chinese (zh)
Inventor
谢彦玲
毛露甜
王熙睿
秦磊
杨涛涛
尹国瑞
梁焯龄
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Huizhou University
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Huizhou University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy

Abstract

The invention belongs to the field of preparation of biological agents, and particularly relates to a sewage treatment agent and a preparation method thereof. On one hand, the properties of the composite material are improved by utilizing the advantages of the performance and the structure of the sodium alginate, and on the other hand, the characteristics of other substances are utilized to improve the sodium alginate, so that the utilization rate of the composite material is improved, and the application range is enlarged. The sodium alginate has the advantages of good film forming property, biodegradability, biocompatibility and the like. The biological agent particles obtained by the invention are easy to form balls, and have the advantages of good elasticity, difficult breakage and difficult deformation.

Description

Sewage treatment microbial inoculum and preparation method thereof
Technical Field
The invention belongs to the field of preparation of biological agents, and particularly relates to a sewage treatment agent, and a preparation method and application thereof.
Background
Organic wastewater contains organic substances such as carbohydrates, proteins, fats and oils, and lignin, and is often present in suspended or dissolved state in wastewater. These organic substances can be decomposed by microorganisms, but cause a decrease in dissolved oxygen in the water, which affects the growth of fish and other aquatic organisms. When the dissolved oxygen in water is exhausted, organic matters are converted into anaerobic decomposition, and then bad smells such as hydrogen sulfide, ammonia, mercaptan and the like are generated, so that the water quality is deteriorated. Therefore, the effective treatment of organic wastewater has obvious environmental benefit and economic benefit for protecting ecological environment and improving the yield of fishery industry.
Photosynthetic bacteria are aquatic microorganisms that perform photosynthesis without producing oxygen by using light as an energy source and carbon dioxide or organic substances as a carbon source. Research shows that photosynthetic bacteria can utilize ammonia nitrogen, phosphate, etc. in water to reduce toxic matter in water, increase dissolved oxygen and raise water transparency, so as to improve water quality and prevent water eutrophication. The composite photosynthetic bacteria group has better degradation effect on organic wastewater than single photosynthetic bacteria or common activated sludge, and the degradation efficiency is higher than 18-39 percent. In the complex flora, the degradation advantage of the photosynthetic bacteria is more obvious. However, free photosynthetic bacteria have a plurality of defects, the antitoxic performance and impact resistance can be effectively improved by adopting an immobilization technology, solid-liquid separation is easy, aeration is not needed, energy consumption is greatly saved, the sludge yield is reduced, the degradation effect is obviously better than that of the free bacteria, the hydraulic retention time is shortened, and the defects of difficult settlement of the free photosynthetic bacteria, small strain concentration, poor environment influence resistance and the like are overcome.
The methods of immobilizing microbial cells are various, and any method of restricting the free flow of microbial cellsThe techniques of (3) can be used for immobilization. Generally, the method can be classified into four major groups, i.e., adsorption, covalent bonding, crosslinking, and entrapment, among which adsorption and entrapment are most commonly used. From the research at home and abroad at present, the immobilization forming machine is mainly designed by adopting the embedding method principle, and the immobilization forming machine is mainly used for producing colloidal particles based on the following three mechanisms: the dropping method, the extrusion method, and the dispersion method. Wherein the dripping method is mainly suitable for preparing calcium alginate gel due to the fact that the calcium alginate gel meets Ca 2+ 、Ba 2+ 、Fe 3+ And the regular sphere is formed in the solution, and the sphere is not influenced by the dropping height, the dropping speed and the concentration of the contact solution within a certain range. At present, the materials for immobilizing photosynthetic bacteria mainly adopt sodium alginate, agar and PVA. In order to balance the advantages and the disadvantages of a single carrier, the composite carrier formed by combining an inorganic carrier and an organic carrier material enables the performances of the two materials to be complementary, thereby showing the advantages of the composite material. For example, for the sodium alginate composite material, on one hand, the properties of the composite material are improved by using the advantages of the performance and the structure of the sodium alginate, and on the other hand, the properties of other substances are also used for improving the sodium alginate, so that the utilization rate of the sodium alginate is improved, and the application range is enlarged. Sodium alginate has the advantages of good film forming property, biodegradability, biocompatibility and the like, and is widely used in systems of wastewater purification, biodegradation, drug slow release, dosing, targeting carriers and the like.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a sewage treatment microbial inoculum and a preparation method and application thereof, which are used as optimization of a microorganism immobilization method, have the advantages of easy balling, good elasticity, difficult fragmentation and difficult deformation.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a sewage treatment microbial inoculum comprises concentrated bacterial liquid and a fixing agent, wherein the concentrated bacterial liquid and the fixing agent are mixed according to the volume ratio of 1: 4-6;
the fixing agent comprises sodium alginate and water, and also comprises at least one of gelatin and zeolite.
Preferably, the fixing agent consists of zeolite, sodium alginate and water, and the weight ratio of the zeolite to the sodium alginate is 1: 1.
Preferably, the concentrated bacterial liquid is concentrated bacterial liquid of photosynthetic bacteria, and the wet weight is 60-70 g/L.
For embedding agent types, the gelatin particle structure is compact, and organic wastewater is not easy to enter the inside of the particles, so that the water quality purification effect is not high. The zeolite particles have a plurality of holes, and metal cations and water molecules in the zeolite particles have adsorption and ion exchange properties, so that substances in the organic wastewater can enter the particles to grow photosynthetic bacteria, and the combination of the zeolite and the sodium alginate is superior to the combination of the gelatin and the sodium alginate. The degradation effect of the combination of the gelatin, the zeolite and the sodium alginate is between the two. The free photosynthetic bacteria have poor treatment effect due to low density, difficult sedimentation and the like.
Preferably, the concentrated bacterial liquid is concentrated bacterial liquid of photosynthetic bacteria, and the dosage form of the microbial inoculum is granules.
The invention also provides a preparation method of the sewage treatment microbial inoculum, which comprises the following steps:
s1, weighing sodium alginate, gelatin and zeolite, adding the sodium alginate, the gelatin and the zeolite into water, and heating and dissolving to obtain a fixing agent;
s2, adding concentrated bacterial liquid into the fixing agent obtained in the step S1 according to the volume ratio of the concentrated bacterial liquid to the fixing agent of 1:4-6 to obtain mixed liquid;
s3, dripping the mixed solution obtained in the step S2 into sterile 2% CaC1 2 Obtaining round particles with the particle size of 1-10mm in the saturated boric acid solution;
s4, placing the round particles obtained in the step S3 into a refrigerator, continuously stabilizing and immobilizing for 24 hours at the temperature of-10-10 ℃, and washing with sterile water to obtain the sewage treatment microbial inoculum particles.
Preferably, the round particles described in step S3 have a particle size of 3-5 mm. Most preferably, the round particles of step S3 have a particle size of 3 mm.
The invention also provides an application of the sewage treatment microbial inoculum for treating sewage.
Preferably, the dosage of the sewage treatment microbial inoculum is 5-20g/L when the sewage treatment microbial inoculum is used for treating sewage.
Preferably, the application condition of the sewage treatment microbial inoculum is 20-40 ℃, the sewage treatment microbial inoculum is anaerobic under illumination for 3-5 days.
The invention has the following beneficial effects:
the invention utilizes the advantages of the performance and the structure of the sodium alginate to improve the property of the composite material, and on the other hand, the invention also utilizes the characteristics of other substances to improve the sodium alginate, thereby improving the utilization rate and expanding the application range. The sodium alginate has the advantages of good film forming property, biodegradability, biocompatibility and the like.
The biological microbial inoculum particles obtained by the sewage treatment microbial inoculum immobilization method provided by the invention are easy to form balls, and have the advantages of good elasticity, low probability of breakage and low probability of deformation.
Detailed Description
The present invention will be described in detail with reference to examples, which are only preferred embodiments of the present invention and are not intended to limit the present invention. The following embodiments are all operated by methods conventional in the art unless otherwise specified.
The sewage treatment bacterial particles for preparing the microbial inoculum described in the embodiment 1-3 are collected from vegetable field bottom mud at the water outlet of the river bridge section of the Huizhou institute, photosynthetic bacteria are enriched and cultured, and the final wet weight of the bacterial liquid is 60.7 g/L. The experimental wastewater is taken from the pond water near Huizhou institute, the water body is green and has precipitate, after filtration, the water temperature is 19 ℃, the pH value is 6.0, COD is 137.2mg/L, NH3-N is 43.15mg/L, and TP is 0.96 mg/L.
The photosynthetic bacteria were immobilized and embedded in the following manner.
Example 1: gelatin + sodium alginate method.
Weighing gelatin 0.36g and sodium alginate 0.48g, dissolving in 20mL water, heating for dissolving, sterilizing, cooling, adding 4mL concentrated bacterial liquid, stirring, and dripping into sterile 2% CaC1 from 10 cm height with 1mL sterile pipette 2 Obtaining round particles with the particle size of 3-8mm in the saturated boric acid solution, namely the sewage treatment microbial inoculum. And (3) putting the immobilized sewage treatment microbial inoculum particles into a refrigerator for continuous stable immobilization for 24 hours. The harvested pellets were washed with sterile water after wet weight measurement and stored in 0.85% physiological saline for later use.
Putting photosynthetic bacteria into 2% CaCl embedded with gelatin and sodium alginate 2 Saturated boric acid solution of (2)After the preparation, the particles are easy to form balls, are light red, have elasticity and are not easy to break. After 24h of immobilization, the particles remained spherical and were not deformed.
Under the condition of light anaerobic reaction, particles with the particle size of 4mm are taken, organic wastewater is added according to the adding amount of 10g/L, the effect of degrading the organic wastewater is measured, the COD removal rate reaches 77.84%, the ammonia nitrogen removal rate reaches 93.96%, and the total phosphorus removal rate is 36.67%.
Example 2: zeolite + sodium alginate method.
Weighing 0.48g of zeolite (ground powder) and 0.48g of sodium alginate, dissolving in 20mL of water, heating for dissolving, sterilizing and cooling, adding 4mL of concentrated bacterial liquid, stirring uniformly, dripping into a saturated boric acid solution of 2% CaC12 from a proper height by using 1mL of a sterile pipetting gun, and carrying out the rest steps as in example 1.
2% CaCl is added into immobilized photosynthetic bacteria with zeolite and sodium alginate as embedding agent 2 After the saturated boric acid solution is added, the particles are easy to form balls, are opaque, deep red, have excellent elasticity and are not easy to break. The elasticity of the particles with different particle diameters is different. After 24h of immobilization, the particles remained spherical and were not deformed.
Under the condition of light anaerobic reaction, particles with the particle size of 3mm are taken, organic wastewater is added according to the adding amount of 10g/L, the effect of degrading the organic wastewater is measured, the removal rate of COD reaches 93.22%, the removal rate of ammonia nitrogen reaches 99.63%, and the removal rate of total phosphorus is 83.00%.
Example 3: gelatin + zeolite + sodium alginate method.
Weighing gelatin 0.48g, zeolite 0.40g, and sodium alginate 0.48g, dissolving in 20mL water, heating for dissolving, sterilizing, cooling, adding 4mL concentrated bacterial liquid, stirring, and dripping 2% CaC1 with 1mL sterile pipette from appropriate height 2 The same procedure as in example 1 was repeated except that the solution was saturated with boric acid.
2% CaCl is added into immobilized photosynthetic bacteria with gelatin, zeolite and sodium alginate as embedding agents 2 After the saturated boric acid solution is added, the particles are very easy to form balls, are opaque, are brownish red, have excellent elasticity and are not easy to break
Under the illumination anaerobic condition, particles with the particle size of 5mm are taken, organic wastewater is added according to the adding amount of 10g/L, the effect of degrading the organic wastewater is measured, the removal rate of COD reaches 77.26%, the removal rate of ammonia nitrogen reaches 88.09%, and the removal rate of total phosphorus reaches 66.67%.
Comparative example 1: free state photosynthetic bacteria.
The concentrated bacterial liquid is directly added into the organic wastewater according to the adding amount of 2g/L, and the effect of degrading the organic wastewater is measured, wherein the removal rate of COD is 77.55%, the removal rate of ammonia nitrogen is 65.52%, and the removal rate of total phosphorus is 12.02%.
The anaerobic condition created by the zeolite particles with a certain particle size is suitable for the growth of the photosynthetic bacteria, and the growth of the photosynthetic bacteria is unfavorable after the particle size is exceeded. Preferably, the optimum particle size is 3 mm. The reason may be that the substances in the organic wastewater are not easy to enter the inside of the granules with the grain diameters of 5mm and 8mm, and the photosynthetic bacteria are possibly in facultative aerobic bacteria, and the oxygen content of the anaerobic environment created by the granules with the grain diameters of 5mm and 8mm is too low to be suitable for the growth of the granules.
Under the illumination anaerobic condition, after the immobilized photosynthetic bacteria agent of 3mm grain size zeolite and sodium alginate is treated for 4 days, the removal rates of COD and NH3-N, TP in the organic wastewater respectively reach 93.22%, 99.67% and 83.00%. The effect of treating the wastewater is obviously better than that of a free microbial inoculum, and the method has an application prospect of treating the organic wastewater on a large scale.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are all within the protection scope of the present invention.
It should be noted that the various features described in the foregoing embodiments may be combined in any suitable manner without contradiction. The invention is not described in detail in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention can be made, and the same should be considered as the disclosure of the present invention as long as the idea of the present invention is not violated.

Claims (9)

1. A sewage treatment microbial inoculum is characterized by comprising concentrated bacterial liquid and a fixing agent, wherein the concentrated bacterial liquid and the fixing agent are mixed according to the volume ratio of 1: 4-6;
the fixing agent comprises sodium alginate and water, and also comprises at least one of gelatin and zeolite.
2. The sewage treatment microbial inoculum according to claim 1, wherein the fixing agent comprises zeolite, sodium alginate and water, and the weight ratio of the zeolite to the sodium alginate is 1: 1.
3. The sewage treatment microbial inoculum according to claim 1, wherein the concentrated bacterial liquid is concentrated bacterial liquid of photosynthetic bacteria, and the wet weight is 60-70 g/L.
4. The sewage treatment microbial inoculum according to claim 1, wherein the dosage form of the microbial inoculum is granules.
5. A method for preparing a sewage treatment microbial inoculum according to any one of claims 1 to 4, which is characterized by comprising the following steps:
s1, weighing sodium alginate, gelatin and zeolite, adding the sodium alginate, the gelatin and the zeolite into water, and heating and dissolving to obtain a fixing agent;
s2, adding concentrated bacterial liquid into the fixing agent obtained in the step S1 according to the volume ratio of the concentrated bacterial liquid to the fixing agent of 1:4-6 to obtain mixed liquid;
s3, dripping the mixed solution obtained in the step S2 into sterile 2% CaC1 2 Obtaining round particles with the particle size of 1-10mm in the saturated boric acid solution;
s4, placing the round particles obtained in the step S3 into a refrigerator, continuously stabilizing and immobilizing for 24h at the temperature of-10-10 ℃, and washing with sterile water to obtain the sewage treatment microbial inoculum particles as claimed in any one of claims 1-3.
6. The method of claim 5, wherein the round particles of step S3 have a diameter of 3-5 mm.
7. The use of a wastewater treatment microbial inoculum according to any one of claims 1 to 4 for treating wastewater.
8. The application of the sewage treatment microbial inoculum according to claim 7, wherein the adding amount is 5-20 g/L.
9. The application of the sewage treatment microbial inoculum according to claim 7, wherein the application condition is 20-40 ℃, the illumination is anaerobic, and the time is 3-5 days.
CN202210723060.0A 2022-06-24 2022-06-24 Sewage treatment microbial inoculum and preparation method thereof Pending CN114921386A (en)

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Application Number Priority Date Filing Date Title
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