CN114903813A - Emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin - Google Patents
Emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin Download PDFInfo
- Publication number
- CN114903813A CN114903813A CN202210411267.4A CN202210411267A CN114903813A CN 114903813 A CN114903813 A CN 114903813A CN 202210411267 A CN202210411267 A CN 202210411267A CN 114903813 A CN114903813 A CN 114903813A
- Authority
- CN
- China
- Prior art keywords
- oligosaccharide
- emulsion
- skin
- relieving
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 229920001542 oligosaccharide Polymers 0.000 title claims abstract description 95
- 150000002482 oligosaccharides Chemical class 0.000 title claims abstract description 63
- 239000000839 emulsion Substances 0.000 title claims abstract description 49
- 206010040849 Skin fissures Diseases 0.000 title claims abstract description 26
- 239000000203 mixture Substances 0.000 title claims abstract description 26
- 241000199919 Phaeophyceae Species 0.000 claims abstract description 20
- 239000000047 product Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 5
- 235000010443 alginic acid Nutrition 0.000 claims description 47
- 229920000615 alginic acid Polymers 0.000 claims description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 33
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 22
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 claims description 22
- 229940072056 alginate Drugs 0.000 claims description 19
- 239000003242 anti bacterial agent Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 15
- 239000003921 oil Substances 0.000 claims description 14
- 235000019198 oils Nutrition 0.000 claims description 14
- -1 alginate oligosaccharide Chemical class 0.000 claims description 13
- 238000001816 cooling Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000004166 Lanolin Substances 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 11
- 229910021641 deionized water Inorganic materials 0.000 claims description 11
- YQEMORVAKMFKLG-UHFFFAOYSA-N glycerine monostearate Natural products CCCCCCCCCCCCCCCCCC(=O)OC(CO)CO YQEMORVAKMFKLG-UHFFFAOYSA-N 0.000 claims description 11
- SVUQHVRAGMNPLW-UHFFFAOYSA-N glycerol monostearate Natural products CCCCCCCCCCCCCCCCC(=O)OCC(O)CO SVUQHVRAGMNPLW-UHFFFAOYSA-N 0.000 claims description 11
- 229940039717 lanolin Drugs 0.000 claims description 11
- 235000019388 lanolin Nutrition 0.000 claims description 11
- 239000004006 olive oil Substances 0.000 claims description 11
- 235000008390 olive oil Nutrition 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- 229920000936 Agarose Polymers 0.000 claims description 10
- 229940008099 dimethicone Drugs 0.000 claims description 7
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 7
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 7
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 claims description 7
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 7
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- AMTWCFIAVKBGOD-UHFFFAOYSA-N dioxosilane;methoxy-dimethyl-trimethylsilyloxysilane Chemical compound O=[Si]=O.CO[Si](C)(C)O[Si](C)(C)C AMTWCFIAVKBGOD-UHFFFAOYSA-N 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 229940083037 simethicone Drugs 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 102000004317 Lyases Human genes 0.000 claims description 3
- 108090000856 Lyases Proteins 0.000 claims description 3
- 229920005654 Sephadex Polymers 0.000 claims description 3
- 239000012507 Sephadex™ Substances 0.000 claims description 3
- 108010045649 agarase Proteins 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000013375 chromatographic separation Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- CBOQJANXLMLOSS-UHFFFAOYSA-N ethyl vanillin Chemical class CCOC1=CC(C=O)=CC=C1O CBOQJANXLMLOSS-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
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- 230000000415 inactivating effect Effects 0.000 claims description 3
- 230000002779 inactivation Effects 0.000 claims description 3
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- 238000002156 mixing Methods 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
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- 239000006228 supernatant Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 3
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 3
- 235000012141 vanillin Nutrition 0.000 claims description 3
- 238000000703 high-speed centrifugation Methods 0.000 claims description 2
- 239000004599 antimicrobial Substances 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 14
- 206010061218 Inflammation Diseases 0.000 abstract description 6
- 208000003251 Pruritus Diseases 0.000 abstract description 6
- 230000004054 inflammatory process Effects 0.000 abstract description 6
- 230000007803 itching Effects 0.000 abstract description 6
- 230000008591 skin barrier function Effects 0.000 abstract description 6
- 230000004071 biological effect Effects 0.000 abstract description 5
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
- 230000003000 nontoxic effect Effects 0.000 abstract description 4
- 230000036559 skin health Effects 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
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- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 4
- 241001474374 Blennius Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
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- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 2
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- 239000002390 adhesive tape Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
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- 229960000890 hydrocortisone Drugs 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
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- 230000008961 swelling Effects 0.000 description 2
- WTFUTSCZYYCBAY-SXBRIOAWSA-N 6-[(E)-C-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-N-hydroxycarbonimidoyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C/C(=N/O)/C1=CC2=C(NC(O2)=O)C=C1 WTFUTSCZYYCBAY-SXBRIOAWSA-N 0.000 description 1
- 241000905957 Channa melasoma Species 0.000 description 1
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- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
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- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000000740 bleeding effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000000548 hind-foot Anatomy 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000001872 metatarsal bone Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 239000000178 monomer Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000011505 plaster Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
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- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
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- 206010040882 skin lesion Diseases 0.000 description 1
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- 210000000106 sweat gland Anatomy 0.000 description 1
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/925—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of animal origin
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- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12P19/00—Preparation of compounds containing saccharide radicals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Emergency Medicine (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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- Rheumatology (AREA)
- Pain & Pain Management (AREA)
- Dispersion Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin, and belongs to the technical field of marine organisms. The skin care emulsion containing the brown algae oligosaccharide and the new agaro-oligosaccharide is safe, non-toxic and high in biological activity, has the effects of protecting and repairing skin barriers, preserving moisture, resisting inflammation and relieving itching, can effectively prevent and relieve skin chap, improve skin activity and restore skin health; the invention prepares the new agaro-oligosaccharide and the brown algae oligosaccharide by an enzymolysis method, the molecular weight is below 2000Da, and the emulsion can quickly permeate the skin surface layer to play the roles of protecting and repairing the skin barrier, preserving moisture, resisting inflammation and relieving itching; the active ingredients are from the sea, and the product is green and safe.
Description
Technical Field
The invention relates to an emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin, belonging to the technical field of marine organisms.
Background
Rhagadia is also called as rhagadia manus et pedis, and refers to a disease of dry skin and linear cracks of skin of hands and feet due to various reasons. Chapping is a common skin condition skin lesion ranging from no sensation of any mild stinging or moderate tenderness to burning pain with bleeding. Some people engaged in special professions, the elderly and women are often stimulated by mechanical or chemical substances, and in addition, the people are cold in winter climate, have reduced secretion of subcutaneous sweat glands, are often rubbed, lose moisture and dryness of skin, thicken skin cutin and lose elasticity, so that the hands and feet are easy to chap when doing exercises.
Some patients with dry hands and feet, especially the elderly, prefer to wrap the dry part with adhesive plaster, which is not a scientific treatment. At present, skin care products on the market are mostly designed by young people, so that the skin requirements of special people are difficult to meet, and the obvious skin care effect cannot be achieved. The application of hormone ointment has great side effect and drug resistance, and the skin care emulsion which is designed for people with special needs and can safely and effectively prevent and relieve chapped skin is urgently needed.
Alginate oligosaccharides (also known as Alginate oligosaccharides) are oligomers obtained by degradation of algin. Algin (Alginate) is a linear polysaccharide polymer formed by linking 2 monomers of beta-1, 4-D-mannuronic acid (M) and alpha-1, 4-L-guluronic acid (G) through 1, 4-glycosidic bonds, mainly comes from brown algae plants such as kelp, gulfweed, kelp and the like, has various physiological activities of resisting tumors, enhancing immunity, promoting growth and the like, but has certain limitation in practical application because the algin (Alginate) is a substance with high polymerization degree, has larger molecular mass, is not easy to dissolve and has higher viscosity. The molecular structure of algin contains a large amount of hydroxyl, and the sugar molecule extension structure can be changed by enzymolysis, so that the sugar hydroxyl is exposed, and the performance of the algin is improved. The algin oligose is a polymer formed by connecting 2-20 monosaccharide molecules through glycosidic bonds as a degradation product of algin, and has the characteristics of good solubility, high body absorption and utilization rate, good stability and the like due to low relative molecular mass.
Agarose is also called as agar sugar or agar, is a natural high molecular linear polysaccharide purified from large-scale seaweed, and the basic structure of the agarose is composed of long chains formed by alternately connecting D-galactose and 3, 6-diether-L-galactose through beta- (1,4) and alpha- (1,3) glycosidic bonds. Agarose, a macroalgae polysaccharide, has many excellent properties such as electric neutrality, gelling property, hysteresis property and the like, but has limited application in many fields such as food, medicine and the like due to the characteristics of high viscosity, large molecular weight, small solubility and the like. The degraded agar oligosaccharide has good water solubility, is beneficial to absorption by human body, not only has the general characteristics of functional oligosaccharide, but also has the physiological function which can not be replaced by a plurality of common oligosaccharides. The agar oligosaccharide has the functions of bacteriostasis, intestinal probiotic proliferation and antivirus on microorganisms, and also has the biological activities of anti-tumor, immunity enhancement, anti-inflammatory action, antioxidation, moisture absorption and moisture retention, whitening and the like, and is an oligosaccharide with great development potential.
The marine polysaccharide has good moisture absorption and retention performance, the moisture retention mechanism is that a large number of hydrophilic groups are contained in molecules, the marine polysaccharide can be associated with water molecules to form hydrogen bonds to combine a large number of water, sugar molecules are mutually interwoven to form a net shape, a layer of film is formed on the surface to play a role in water retention, after the polysaccharide is degraded into oligosaccharide, a large number of free amino groups are released along with the breakage of glycosidic bonds, the capacity of combining the water molecules can be enhanced, and in addition, the molecular structure becomes a loose state due to the breakage of the molecules, so that the approach of the water molecules is facilitated. Research shows that the brown algae oligosaccharide and the neoagaro oligosaccharide prepared by the enzymolysis method have good moisture-preserving and moisture-absorbing properties. The brown algae oligosaccharide and the new agaro-oligosaccharide also have good antioxidant activity, and the antioxidant activity is enhanced by matching the brown algae oligosaccharide and the new agaro-oligosaccharide, so that the damaged skin is repaired. The invention relates to an enzymolyzed skin care emulsion of alginate oligosaccharides and neoagaro-oligosaccharides, which is safe, nontoxic and high in biological activity, has the functions of protecting and repairing skin barriers, moisturizing, resisting inflammation and relieving itching, can effectively prevent and relieve chapped skin, improve skin activity and restore skin health.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides an emulsion of a marine oligosaccharide composition for preventing and relieving chapped skin and a preparation method thereof. The skin care emulsion of brown algae oligosaccharide and neoagaro oligosaccharide with the molecular weights of less than 2000 is safe, non-toxic and high in biological activity, has the effects of protecting and repairing skin barriers, moisturizing, resisting inflammation and relieving itching, and can effectively prevent and relieve chapped skin, improve skin activity and restore skin health.
The invention realizes the technical effects through the following technical scheme:
an emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 0-10% of neoagaro-oligosaccharide, 0-5% of alginate-derived oligosaccharide, 3-10% of glycerol and enough deionized water;
(2) oil phase: 2-10% of lanolin, 1-6% of glycerol monostearate, 2-10% of simethicone, 0.5-5% of octadecanol and 1-10% of olive oil;
(3) dressing: 0.1-1% of antibacterial agent.
Preferably, the emulsion added with the marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 3-7% of neoagaro-oligosaccharide, 2-4% of alginate-derived oligosaccharide, 5-8% of glycerol and enough deionized water;
(2) oil phase: 3-6% of lanolin, 1-3% of glycerol monostearate, 3-5% of simethicone, 2-3% of octadecanol and 2-5% of olive oil;
(3) dressing: 0.1-1% of an antibacterial agent.
Preferably, the emulsion added with the marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 4% of new agaro-oligosaccharide, 3% of brown algae oligosaccharide, 6% of glycerol and 71.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Further, the new agaro-oligosaccharide is prepared by the following steps:
(1) accurately preparing 0.01-0.5% agarose solution, and heating at 90-110 ℃ to completely dissolve the agarose solution;
(2) cooling to 35-45 ℃, adding 90-110U agarase Aga into every 90-110 mL of substrate, uniformly mixing, and carrying out oscillation reaction at 40-60 ℃ and 100-200 rpm for 2-8 h;
(3) heating in boiling water bath for 5-15 min to inactivate enzyme protein, and completely cooling at 0-10 deg.C for settling;
(4) centrifuging at 0-10 ℃ for 5-15 min at 5000-15000 r/min to remove insoluble substances;
(5) concentrating the sample volume by using a rotary evaporator, and standing overnight at 0-10 ℃;
(6) centrifuging at 0-10 ℃ at 5000-15000 r/min for 2-8 min, and filtering the supernatant with a 0.22um filter membrane to remove impurities;
(7) freeze drying to powder, and storing in dry place.
Further, the brown algae oligosaccharide is prepared by the following steps:
(1) dissolving algin: dissolving 15-25 g of algin in 1500-2500 ml of distilled water to obtain 0.5-1.5% algin solution, and heating and stirring at 50-70 ℃ on a magnetic stirrer to completely dissolve the algin solution;
(2) carrying out enzymolysis on algin: adding 5-15 ml of dissolved algin solution into algin lyase, and reacting for 3-8 h at 55-65 ℃ on a magnetic stirrer;
(3) inactivation of enzymolysis liquid: inactivating the liquid after enzymolysis at the temperature of 80-100 ℃ for 15-35 min;
(4) and (3) centrifuging enzymolysis liquid: performing high-speed centrifugation on the enzymolysis product at 8000-14000 r/min for 5-15 min;
(5) freeze-drying enzymolysis liquid: vacuum freeze-drying the enzymolysis liquid to obtain brown algae oligosaccharide powder;
(6) preparing the low-polybrown alginate oligosaccharide: and (3) carrying out chromatographic separation on the enzymolyzed alginate oligosaccharides by using a Sephadex gel column, collecting alginate oligosaccharide components, concentrating and drying.
Preferably, the antibacterial agent is an organic antibacterial agent harmless to human body, and is mainly vanillin or ethyl vanillin compounds.
The invention also provides a preparation method of the emulsion added with the marine oligosaccharide composition for preventing and relieving chapped skin, which specifically comprises the following steps: heating the water phase and oil phase to 75 deg.C respectively, emulsifying at high speed for 5min by alternative addition method, cooling to 50 deg.C, adding antibacterial agent, stirring, cooling to room temperature to obtain marine oligosaccharide composition emulsion, and packaging to obtain emulsion product.
The invention provides an emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin, which has the following remarkable advantages compared with the prior art:
(1) the skin care emulsion containing the brown algae oligosaccharide and the new agaro-oligosaccharide is safe, non-toxic and high in biological activity, has the effects of protecting and repairing skin barriers, preserving moisture, resisting inflammation and relieving itching, can effectively prevent and relieve skin chap, improve skin activity and restore skin health;
(2) the invention prepares new agaro-oligosaccharide and brown algae oligosaccharide by enzymolysis, the molecular weight is below 2000Da, the emulsion can quickly permeate the skin surface layer, and the effects of protecting and repairing skin barrier, moisturizing, resisting inflammation and relieving itching are achieved, and the experimental result shows that the product has obvious technical effect;
(3) the emulsion for preventing and relieving the chapped skin is prepared by utilizing the natural seaweed oligosaccharide, the active ingredients are from the sea, and the product is green and safe.
Detailed Description
In the following examples, the new agaro-oligosaccharides were prepared by the following steps:
(1) accurately preparing 0.2% agarose solution, and heating at 90-110 ℃ to completely dissolve the agarose solution; wherein the agarose is a Spanish BIOWEST product;
(2) cooling to 35-45 ℃, adding 100U agarase Aga into each 100mL of substrate, uniformly mixing, and carrying out oscillation reaction at 40-60 ℃ and 150rpm for 6 hours;
(3) heating in boiling water bath for 10min to inactivate enzyme protein, and completely cooling at 0-10 deg.C for settling;
(4) centrifuging at 0-10 ℃ at 10000r/min for 10min to remove insoluble substances;
(5) concentrating the sample volume by using a rotary evaporator, and standing overnight at 0-10 ℃;
(6) centrifuging at 0-10 ℃ at 10000r/min for 6min, and filtering the supernatant with a 0.22um filter membrane to remove impurities;
(7) freeze-drying to obtain powder, and storing in dry place.
In the following examples, the brown algae oligosaccharides were prepared by the following steps:
(1) dissolving algin: dissolving 20 g of algin in 2000ml of distilled water to obtain a 1.0% algin solution, and heating and stirring the solution on a magnetic stirrer at 50-70 ℃ to completely dissolve the algin solution;
(2) carrying out enzymolysis on algin: adding 5-15 ml of dissolved algin solution into algin lyase, and reacting for 5h at 55-65 ℃ on a magnetic stirrer;
(3) inactivation of enzymolysis liquid: inactivating the liquid after enzymolysis at 80-100 ℃ for 25 min;
(4) and (3) centrifuging enzymolysis liquid: centrifuging the enzymolysis product at 11000r/min at high speed for 10 min;
(5) freeze-drying enzymolysis liquid: vacuum freeze-drying the enzymolysis liquid to obtain brown algae oligosaccharide powder;
(6) preparing the low-polybrown alginate oligosaccharide: and (3) carrying out chromatographic separation on the enzymolyzed alginate oligosaccharides by using a Sephadex gel column, collecting alginate oligosaccharide components, concentrating and drying.
In the following examples, the antibacterial agent is an organic antibacterial agent harmless to human body, wherein the antibacterial agent is a mixture of vanillin and ethyl vanillin compounds, and the mass ratio is 1: 1.
In the following examples, the preparation method of the emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin specifically comprises the following steps: heating the water phase and oil phase to 75 deg.C respectively, emulsifying at high speed for 5min by alternative addition method, cooling to 50 deg.C, adding antibacterial agent, stirring, cooling to room temperature to obtain marine oligosaccharide composition emulsion, and packaging to obtain emulsion product.
Example 1
An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 4% of new agaro-oligosaccharide, 3% of brown algae oligosaccharide, 6% of glycerol and 71.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Example 2
An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 3% of new agaro-oligosaccharide, 4% of brown algae oligosaccharide, 6% of glycerol and 71.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Example 3
An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 3% of alginate oligosaccharide, 6% of glycerol and 75.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Example 4
An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 4% of new agaro-oligosaccharide, 6% of glycerol and 74.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Example 5
An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin comprises the following raw materials in parts by weight:
(1) water phase: 6% of glycerol and 78.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
Example 6 moisturizing Effect test
The adhesive tape is used for simulating biological materials such as cuticle, epidermis and the like, and skin care emulsion is coated on the adhesive tape to simulate the application condition of an actual skin care product. Weighing mass difference of the sample before and after the sample is placed for a certain time under the conditions of constant temperature and constant humidity, and calculating the loss of the sample amountThe effect of the moisturizing component on moisturization can be calculated. Moisture retention rate calculation formula: moisture retention rate = (M) 2 -M 0 ）/(M 1 -M 0 ) X 100% of formula: m 0 Empty plate mass/g; m 1 The weight/g of the glass plate after sample adding; m 2 Mass/g after standing in a desiccator for some time. The moisture retention rates were tested at 43% relative humidity for 4h and 8 h. The test results (table 1) are as follows:
table 1: moisturizing ratio of skin care emulsions of examples and comparative examples
| Time/h | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 |
| 4 | 40.5 | 35.5 | 26.3 | 30.3 | 15.6 |
| 8 | 37.6 | 30.9 | 19.6 | 24.5 | 12.4 |
As can be seen from Table 1, the skin care emulsions described in the examples have certain moisturizing effects on skin, but the moisturizing effects of the skin care emulsions of examples 1-2 added with the brown alga oligosaccharides and the new agaro oligosaccharides on skin are obviously better than the moisturizing effects of examples 3-4 added with single oligosaccharides, which shows that the brown alga oligosaccharides and the new agaro oligosaccharides added in the examples of the invention have mutual synergistic effect, the moisture absorption and water locking functions of the product are improved, and the skin care emulsions of the invention have remarkable moisturizing and water locking functions. By way of comprehensive comparison, example 1 is the best embodiment of the present invention.
Example 7 anti-inflammatory Effect test
Healthy SD rats, 30 in each half of males and females, were randomly divided into three groups, namely a blank control group, a positive control group and an experimental group (example 1), and 10 rats were selected. The right hind metatarsal of the rat was injected subcutaneously with 0.1ml of 1% carrageenan to inflame, and immediately the test samples were evenly applied to the area under the nude joint, the blank control group was applied with 0.2ml of 5% propylene glycol physiological saline per mouse, the positive control group was applied with 0.2g of hydrocortisone cream per mouse, the test group was applied with 0.2g of the skin care emulsion of example 1 per mouse, and the perimeter of the right hind paw of the SD rat was measured at 1h,2h, and 3h after administration.
TABLE 2 Effect on carrageenan-induced swelling in SD rat groups
The results show that compared with the blank control group, the local external skin-care emulsion and the hydrocortisone cream can obviously relieve the swelling of the SD rat group caused by carrageenan, and the difference is obvious; the action lasts for 1-3h, so that the skin care emulsion added with the brown algae oligosaccharide and the new agaro oligosaccharide has obvious anti-inflammatory effect.
The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Claims (7)
1. An emulsion added with a marine oligosaccharide composition for preventing and relieving chapped skin is characterized in that the emulsion is prepared from the following raw materials:
(1) water phase: 0-10% of neoagaro-oligosaccharide, 0-5% of alginate-derived oligosaccharide, 3-10% of glycerol and enough deionized water;
(2) oil phase: 2-10% of lanolin, 1-6% of glycerol monostearate, 2-10% of simethicone, 0.5-5% of octadecanol and 1-10% of olive oil;
(3) dressing: 0.1-1% of an antibacterial agent.
2. The emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin as claimed in claim 1, wherein the emulsion is prepared from the following raw materials:
(1) water phase: 3-7% of neoagaro-oligosaccharide, 2-4% of alginate-derived oligosaccharide, 5-8% of glycerol and enough deionized water;
(2) oil phase: 3-6% of lanolin, 1-3% of glycerol monostearate, 3-5% of simethicone, 2-3% of octadecanol and 2-5% of olive oil;
(3) dressing: 0.1-1% of an antibacterial agent.
3. The emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin as claimed in claim 1, wherein the emulsion is prepared from the following raw materials:
(1) water phase: 4% of new agaro-oligosaccharide, 3% of brown algae oligosaccharide, 6% of glycerol and 71.5% of deionized water;
(2) oil phase: lanolin 4%, glycerol monostearate 2%, dimethicone 4%, stearyl alcohol 2%, and olive oil 3%;
(3) dressing: 0.5 percent of antibacterial agent.
4. The emulsion with the addition of marine oligosaccharide composition for preventing and relieving chapped skin as claimed in claim 1, wherein said neoagaro-oligosaccharide is prepared by the following steps:
(1) accurately preparing 0.01-0.5% agarose solution, and heating at 90-110 ℃ to completely dissolve the agarose solution;
(2) cooling to 35-45 ℃, adding 90-110U agarase Aga into every 90-110 mL of substrate, uniformly mixing, and carrying out oscillation reaction at 40-60 ℃ and 100-200 rpm for 2-8 h;
(3) heating in boiling water bath for 5-15 min to inactivate enzyme protein, and completely cooling at 0-10 deg.C for settling;
(4) centrifuging at the temperature of 0-10 ℃ at the speed of 5000-15000 r/min for 5-15 min to remove insoluble substances;
(5) concentrating the sample volume by using a rotary evaporator, and standing overnight at 0-10 ℃;
(6) centrifuging at 0-10 ℃ at 5000-15000 r/min for 2-8 min, and filtering the supernatant with a 0.22um filter membrane to remove impurities;
(7) freeze drying to powder, and storing in dry place.
5. The emulsion with the addition of marine oligosaccharide composition for preventing and relieving chapped skin as claimed in claim 1, wherein said brown algae oligosaccharide is prepared by the following steps:
(1) dissolving algin: dissolving 15-25 g of algin in 1500-2500 ml of distilled water to obtain 0.5-1.5% algin solution, and heating and stirring at 50-70 ℃ on a magnetic stirrer to completely dissolve the algin solution;
(2) carrying out enzymolysis on algin: adding 5-15 ml of dissolved algin solution into algin lyase, and reacting for 3-8 h at 55-65 ℃ on a magnetic stirrer;
(3) inactivation of enzymolysis liquid: inactivating the liquid after enzymolysis at the temperature of 80-100 ℃ for 15-35 min;
(4) and (3) centrifuging enzymolysis liquid: performing high-speed centrifugation on the enzymolysis product at 8000-14000 r/min for 5-15 min;
(5) freeze-drying enzymolysis liquid: vacuum freeze-drying the enzymolysis liquid to obtain brown algae oligosaccharide powder;
(6) preparing the low-polybrown alginate oligosaccharide: and (3) carrying out chromatographic separation on the enzymolyzed alginate oligosaccharides by using a Sephadex gel column, collecting alginate oligosaccharide components, concentrating and drying.
6. The emulsion of claim 1, wherein the antimicrobial agent is an organic antimicrobial agent harmless to human body, and is mainly vanillin or ethyl vanillin compounds.
7. The emulsion added with marine oligosaccharide composition for preventing and relieving chapped skin as claimed in claim 1, wherein the preparation method of the emulsion comprises: heating the water phase and oil phase to 75 deg.C respectively, emulsifying at high speed for 5min by alternative addition method, cooling to 50 deg.C, adding antibacterial agent, stirring, cooling to room temperature to obtain marine oligosaccharide composition emulsion, and packaging to obtain emulsion product.
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