CN114894309A - Homogenization light path structure based on fluorescence hyperspectral imaging system - Google Patents
Homogenization light path structure based on fluorescence hyperspectral imaging system Download PDFInfo
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- 238000000265 homogenisation Methods 0.000 title claims abstract description 33
- 238000000701 chemical imaging Methods 0.000 title claims abstract description 18
- 238000012360 testing method Methods 0.000 claims abstract description 35
- 150000001875 compounds Chemical class 0.000 claims abstract description 17
- 229910052724 xenon Inorganic materials 0.000 claims description 5
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 5
- 230000005284 excitation Effects 0.000 description 16
- 230000003287 optical effect Effects 0.000 description 14
- 238000005286 illumination Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000013307 optical fiber Substances 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 238000012634 optical imaging Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0289—Field-of-view determination; Aiming or pointing of a spectrometer; Adjusting alignment; Encoding angular position; Size of measurement area; Position tracking
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0291—Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a homogenization light path structure based on a fluorescence hyperspectral imaging system, relates to the technical field of homogenization light paths, and aims to realize the homogenization light path structure suitable for full-wave band light, and the homogenization light path structure comprises a collimation light guide lens barrel, a light filter, 2 compound eye lens groups, a focusing lens and a plane reflector; the light source is aligned to the light inlet of the collimation light guide lens barrel, the front end of the collimation light guide lens barrel is provided with a light filter slot, the light filter is inserted into the light filter slot, and the light outlet of the collimation light guide lens barrel is inserted into the test box; a first fly-eye lens group, a second fly-eye lens group and a focusing lens which are parallel to each other are sequentially arranged at the light outlet of the collimation light guide lens barrel along a light path; the focusing lens is aligned with the plane reflector, and the plane reflector is arranged at the top of the test box; the plane reflector is used for reflecting light to the sample stage in the test box; the invention has the advantages of improving the homogenization capability and the application range of the homogenization light path structure.
Description
Technical Field
The invention relates to the technical field of homogenization light paths, in particular to the technical field of homogenization light path structures based on a fluorescence hyperspectral imaging system.
Background
An application mode combining a hyperspectral imaging technology and a fluorescence detection technology is an important technical means for detecting the fluorescence characteristic of a target, when a fluorescent substance is excited by specific external energy, an electron orbit in an internal composition structure of the substance can be caused to jump to a high-energy orbit, and finally, a fluorescence signal which can be detected can be generated in the process of releasing energy and returning to a ground state. Of course, not all the substances can be excited to generate fluorescence signals, and the fluorescence signals can be detected by the hyperspectral camera of the optical equipment only when the detected substances have the same frequency as the exciting light and have high fluorescence efficiency after absorbing the changed energy instead of consuming the energy in the intermolecular collision process.
In fluorescence hyperspectral imaging, an excitation light source is an important link of technical application, and commonly used light sources include: a laser light source: the single-wavelength discontinuous light has higher resolution and sensitivity; light emitting diode light source: the excitation light bandwidth is relatively wide (greater than 60nm), the energy output is relatively low, the space between the light source and the sample needs to be relatively close, the optical path is short, but the volume is small, light and economical, and the shortest wavelength of the excitation light is generally greater than 430 nm. Xenon lamp light sources from ultraviolet light to visible-near infrared bands have higher output energy and halogen lamp light sources with wider spectral ranges, each excitation light source has the characteristics and the defects of the excitation light source, and the selection of the excitation light source is very important for detecting different fluorescent signals.
On this basis, the homogenization light source structure designed aiming at the introduction of the light source is the most important technical point, for example, a homogenization columnar light guide pipe is usually adopted, the light guide pipe transmits light from the light guide pipe inlet to the light guide pipe outlet by utilizing Total Internal Reflection (TIR), and the light guide pipe usually has an applicable wavelength range for the entering light, has certain limitation and is difficult to meet the application of full-wave-band hyperspectrum.
Disclosure of Invention
The invention aims to: the homogenization light path structure suitable for full-wave band light is realized. In order to solve the technical problem, the invention provides a homogenization light path structure based on a fluorescence hyperspectral imaging system.
The invention specifically adopts the following technical scheme for realizing the purpose:
a homogenization light path structure based on a fluorescence hyperspectral imaging system is arranged between a light source and a test box, wherein the test box is a dark box and comprises a collimation light guide lens barrel, a light filter, 2 compound eye lens groups, a focusing lens and a plane reflector;
the light source is aligned to the light inlet of the collimation light guide lens barrel, the front end of the collimation light guide lens barrel is provided with an optical filter slot, an optical filter is detachably arranged in the optical filter slot, and the tail end of the collimation light guide lens barrel is inserted into the test box;
the tail end of the collimation light guide lens barrel is sequentially provided with a first fly-eye lens group, a second fly-eye lens group and a focusing lens which are parallel to each other along a light path; the focusing lens is aligned with the plane reflector, and the plane reflector is arranged at the top of the test box; the plane reflector is used for reflecting light to the sample stage in the test box.
Preferably, the fly-eye lens group comprises 9 closely-arranged unit lenses, and nine unit lenses are arranged in a 3X3 manner.
Preferably, each unit lens has a size of 5mmX5 mm.
Preferably, an adjusting structure is arranged at the top of the first fly-eye lens group, and the adjusting structure is used for adjusting the distance between the first fly-eye lens group and the second fly-eye lens group in the horizontal direction.
Preferably, the adjusting structure includes a knob, a main shaft and an auxiliary shaft, the knob is fixed on the top of the main shaft, the main shaft extends into the small hole formed on the body of the collimation light guide lens barrel, the auxiliary shaft is connected to the side surface of the bottom of the main shaft, a transverse strip-shaped limiting opening is arranged on the top of the first compound eye lens group along the length direction of the first compound eye lens group, and the bottom of the auxiliary shaft is connected to the first compound eye lens group through the limiting opening in a sliding manner.
Preferably, an adjusting structure is arranged on the top of the focusing lens, and the adjusting structure is used for adjusting the distance between the focusing lens and the second fly-eye lens group in the horizontal direction.
Preferably, the adjusting structure comprises a knob, a main shaft and an auxiliary shaft, the knob is fixed at the top of the main shaft, the main shaft extends into a small hole formed in the body of the collimation light guide lens barrel, the side surface of the bottom of the main shaft is connected with the auxiliary shaft, a transverse strip-shaped limiting opening is formed in the top of the focusing lens along the length direction of the top of the focusing lens, and the bottom of the auxiliary shaft is connected to the focusing lens through the limiting opening in a sliding mode.
Preferably, the light source adopts a xenon lamp light source.
The invention has the following beneficial effects:
the homogenization light path structure adopted by the invention optimizes the transmission path, thereby reducing the loss of maximum light intensity; the compound eye lens group is used for realizing the homogenization light path, so that the efficiency of the homogenization light path is improved, and the homogenization effect is improved; the invention can obtain light with single wavelength, uniform illumination and uniform intensity distribution; the invention is suitable for light of all wave bands, can be expanded to a wider spectral range and application test requirements, the light source can be replaced according to the test requirements, and the designed homogenization light path does not need to be changed, thus greatly improving the utilization rate of the structure; the optical filter inserted in the slot is adopted, the composite light can be selected through simple operation, and the light with specific wavelength is filtered out, so that a test sample in the test box can be excited; a larger area of uniform light can be provided.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of a first fly-eye lens group and an adjustment structure in embodiment 3;
reference numerals: 101-light source, 102-collimation light guide lens barrel, 103-optical filter, 104-first fly-eye lens group, 105-second fly-eye lens group, 106-focusing lens, 107-plane reflector, 108-test box, 201-knob, 202-main shaft, 203-auxiliary shaft and 204-unit lens.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1, the present embodiment provides a homogenization light path structure based on a fluorescence hyperspectral imaging system, which is disposed between a light source 101 and a test box 108, where the test box 108 is a dark box and includes a collimation light guide lens barrel 102, a filter 103, 2 fly-eye lens groups, a focusing lens 106, and a plane mirror 107;
the light source 101 is aligned with a light inlet of the collimation light guide lens barrel 102, a light filter 103 slot is arranged at the front end of the collimation light guide lens barrel 102, the light filter 103 is detachably mounted in the light filter 103 slot, and the tail end of the collimation light guide lens barrel 102 is inserted into the test box 108;
the tail end of the collimation light guide lens barrel 102 is sequentially provided with a first fly-eye lens group 104, a second fly-eye lens group 105 and a focusing lens 106 which are parallel to each other along a light path; the focusing lens 106 is aligned with a plane mirror 107, and the plane mirror 107 is installed at the top of the test box 108; the plane mirror 107 is used to reflect light to the sample stage in the test chamber 108.
The working principle is as follows:
the homogenizing light path structure of the embodiment is disposed between the light source 101 and the testing box 108, and is used for homogenizing the light source 101 for testing and then conducting the homogenized light to the testing box 108, so as to provide the uniform light source 101 for the sample to be tested in the testing box 108. In summary, the scheme performs optimization design on the aspects of the excitation light source 101, the excitation light transmission path, the transmission effect and the like, under the condition that the brightness of the excitation light is not lost, the light output by the excitation light source 101 sequentially passes through the designed compound eye lens group to achieve the homogenization effect and efficiency of the incident light, then the light energy transmitted through the light path behind the focusing lens 106 and the plane reflector 107 reaches the surface of the test sample, the sample is excited to generate fluorescence, and the fluorescence signal is collected by the hyperspectral camera after passing through the emission filter.
In short, after laser emitted from the light source 101 is collimated and adjusted, a specific wavelength is filtered by the optical filter 103, and specifically, the selection of the optical filter 103 is determined according to the wavelength requirement; the light filtered out by the optical filter 103 passes through the first fly-eye lens group 104 and the second fly-eye lens group 105 in sequence, is adjusted into a uniform collimation light path, then passes through the focusing lens 106 to perform focus imaging to obtain a light source 101 spot, and finally is reflected by the plane reflector 107, so that the light source 101 image at the focus is clearly displayed on a test sample on a test bench in the test box 108.
The design of the homogenization light path structure mainly aims to solve some problems existing in the application of the current fluorescence hyperspectral imaging technology, such as: the excitation light source 101 cannot provide a continuous, arbitrary wavelength, structure, and simplicity of operation; in the traditional structure, because the field of view of the optical fiber or the optical waveguide has limitation, large-area irradiation light spots cannot be provided; under the coordination of a non-focusing lens, the optical fiber or the optical waveguide cannot provide excitation incident light information with enough intensity and excellent uniformity; after light output by the optical fiber or the optical waveguide is reflected by the plane reflector 107, the light is uniformly irradiated on each part on the sample, and the incident light directly irradiates the sample to bring contact damage; therefore, the light path in the fluorescence hyperspectral system is optimally designed, the compound eye lens group can be fully utilized as a medium for optical signal transmission, and the loss of light energy is reduced; the compound eye lens group can be constructed by utilizing a lens group lens with high flux, so that the damage caused by exciting light is reduced; the excitation light passes through a compound eye lens group with a high numerical aperture, and the homogenization structure further homogenizes the excitation light; the homogenized excitation light is acted by the focusing lens 106, so that the emergent light spot can be clearly imaged.
The first fly-eye lens group 104 adopted in this embodiment can form an area array layout of a micro focusing lens 106, so that the focal point of the convergent light spot emitted by the light source 101 such as a xenon lamp can be accurately imaged on the second fly-eye lens group 105, an optical imaging structure with a larger area, higher fine division, higher passing efficiency and wider spectral range response is formed, and finally, the sample is efficiently irradiated with the optical imaging structure to form exciting light.
In particular, after the excitation light after homogenizing the light path is obtained by the present embodiment, the incident light irradiated onto the sample to be tested is a single-wavelength light with uniform illumination and uniform intensity distribution, and the image collected by the detector in the test box 108 is also a sample single-wavelength fluorescence image.
Example 2
The present embodiment is based on the technical solution of embodiment 1, that is, the present embodiment includes a collimating light guide barrel 102, a filter 103, 2 fly-eye lens groups, a focusing lens 106, and a plane mirror 107; the light source 101 is aligned with a light inlet of the collimation light guide lens barrel 102, a light filter 103 slot is arranged at the front end of the collimation light guide lens barrel 102, the light filter 103 is detachably mounted in the light filter 103 slot, and the tail end of the collimation light guide lens barrel 102 is inserted into the test box 108; the tail end of the collimation light guide lens barrel 102 is sequentially provided with a first fly-eye lens group 104, a second fly-eye lens group 105 and a focusing lens 106 which are parallel to each other along a light path; the focusing lens 106 is aligned with a plane mirror 107, and the plane mirror 107 is installed at the top of the test box 108; the plane mirror 107 is used to reflect light to the sample stage in the test chamber 108.
As a preferable scheme, referring to the first fly-eye lens group 104 of fig. 2, the second fly-eye lens group 105 has the same structure, and the fly-eye lens group includes 9 closely arranged unit lenses 204, nine unit lenses 204 are arranged in a 3X3 arrangement;
further, each unit lens 204 has a size of 5mmX5 mm.
First, it can be seen that the two fly-eye lens groups are arranged in parallel, the focal point of each unit lens 204 in the first fly-eye lens group 104 coincides with the center of the corresponding unit lens 204 in the second fly-eye lens group 105, and the optical axes of the two lines of fly-eye lenses are parallel to each other, and the working principle is as follows:
the first fly-eye lens group 104 processes the emitted wider light source 101 into a plurality of thinner light sources 101, and each unit lens 204 of the second fly-eye lens group 105 superposes and images the light emitted from the corresponding unit lens 204 in the first fly-eye lens group 104 on the illumination surface. Because the first fly-eye lens group 104 divides the whole wide light beam of the light source 101 into a plurality of beamlets for illumination, and each beamlet has vertical-axis nonuniformity in the range, but because the beamlets are mutually overlapped at the symmetrical positions, the vertical-axis nonuniformity of the beamlets is compensated, and the light energy in the whole aperture is effectively and uniformly utilized. The light spot emitted from the second fly-eye lens group 105 is focused on the plane reflector 107 through the focusing lens 106, so that each point of the light spot on the plane reflector 107 is irradiated by the light emitted from all points of the light source 101, and simultaneously, the light beams emitted from each point of the light source 101 are intersected and overlapped in the same field of view on the illumination light spot, so that a uniform square light spot is obtained.
Example 3
This embodiment is based on the technical solution of embodiment 2, and the same structure and principle are not described again.
As a preferable aspect of the present embodiment, an adjusting structure for adjusting a distance between the first fly-eye lens group 104 and the second fly-eye lens group 105 in a horizontal direction is disposed at a top of the first fly-eye lens group 104.
Further, as shown in fig. 2, the adjusting structure includes a knob 201, a main shaft 202 and an auxiliary shaft 203, the knob 201 is fixed to the top of the main shaft 202, the main shaft 202 extends into a small hole formed in the body of the collimation light guide lens barrel 102, the side surface of the bottom of the main shaft 202 is connected to the auxiliary shaft 203, a transverse strip-shaped limiting opening is formed in the top of the first compound eye lens group 104 along the length direction of the first compound eye lens group, and the bottom of the auxiliary shaft 203 is connected to the first compound eye lens group 104 through the limiting opening in a sliding manner.
On the other hand, the top of the focusing lens 106 is provided with an adjusting structure for adjusting the distance between the focusing lens 106 and the second fly-eye lens group 105 in the horizontal direction; the specific structure and the leaf principle of the adjusting structure are completely consistent with the adjusting structure for adjusting the first fly-eye lens group 104, and may include a knob 201, a main shaft 202 and a secondary shaft 203, the knob 201 is fixed on the top of the main shaft 202, the main shaft 202 extends into a small hole formed on the body of the collimating and light-guiding lens barrel 102, the secondary shaft 203 is connected to the bottom side surface of the main shaft 202, a transverse strip-shaped limiting opening is arranged on the top of the focusing lens 106 along the length direction thereof, and the bottom of the secondary shaft 203 is slidably connected to the focusing lens 106 through the limiting opening.
Preferably, the light source 101 is a xenon lamp light source 101.
The design idea of the embodiment is as follows:
in the structural design, because of errors such as mechanical errors, the unit lens 204 formed by the first fly-eye lens group 104 is overlapped and imaged and cannot be accurately mapped onto each corresponding unit lens 204 on the second fly-eye lens group 105, in the scheme of the embodiment, the first fly-eye lens group 104 can be finely adjusted to realize direct spatial correction of the two, when in specific operation, a worker can rotate and screw the knob 201 by 360 degrees, the main shaft 202 rotates synchronously with the knob 201, and further the main shaft 202 drives the auxiliary shaft 203 on the bottom side surface to move together, the main shaft 202 and the auxiliary shaft 203 form a crankshaft together, that is, with the uninterrupted rotation of the knob 201, the auxiliary shaft 203 forms a circular motion with the main shaft 202 as the center, and meanwhile, because of the transverse strip-shaped limiting groove at the top of the first fly-eye lens group 104, the motion of the auxiliary shaft 203 is actually limited, and then only the first fly-eye lens group 104 is driven to do reciprocating linear motion in the horizontal direction, the moving direction is perpendicular to the transverse bar limiting groove, namely the distance between the first fly-eye lens group 104 and the second fly-eye lens group 105 is adjusted, in the embodiment, an adjustable stroke of plus or minus 0.5mm can be preferably formed through the size design of the adjusting structure, and the second fly-eye lens group 105 is fixed.
Based on the same adjustment principle, the focusing lens 106 is also designed into the same structure capable of fine adjustment, because the image formed by the second fly-eye lens group 105 may not find a proper focus, the adjustment of the spatial distance between the two is realized through the adjustment structure, and similarly, the focusing lens 106 can also form an adjustable stroke of plus or minus 0.5mm by taking the central position of the second fly-eye lens as a reference.
Claims (8)
1. A homogenization light path structure based on a fluorescence hyperspectral imaging system is arranged between a light source (101) and a test box (108), the test box (108) is a dark box, and the homogenization light path structure is characterized by comprising a collimation light guide lens barrel (102), a light filter (103), 2 compound eye lens groups, a focusing lens (106) and a plane reflector (107);
the light source (101) is aligned to a light inlet of the collimation light guide lens barrel (102), a light filter (103) slot is arranged at the front end of the collimation light guide lens barrel (102), the light filter (103) is detachably mounted in the light filter (103) slot, and the tail end of the collimation light guide lens barrel (102) is inserted into the test box (108);
the tail end of the collimation light guide lens barrel (102) is sequentially provided with a first fly-eye lens group (104), a second fly-eye lens group (105) and a focusing lens (106) which are parallel to each other along a light path; the focusing lens (106) is aligned with a plane mirror (107), and the plane mirror (107) is installed at the top of the test box (108) and used for reflecting light to a sample table in the test box (108).
2. The homogenization light path structure of the fluorescence-based hyperspectral imaging system according to claim 1, wherein the fly-eye lens group comprises 9 closely arranged unit lenses (204), and nine unit lenses (204) are arranged in an arrangement of 3X 3.
3. The homogenization light path structure based on the fluorescence hyperspectral imaging system according to claim 2, characterized in that the size of each unit lens (204) is 5mmX5 mm.
4. The homogenization light path structure of the fluorescence-based hyperspectral imaging system according to claim 1, wherein an adjusting structure is arranged on the top of the first fly-eye lens group (104), and the adjusting structure is used for adjusting the distance between the first fly-eye lens group (104) and the second fly-eye lens group (105) in the horizontal direction.
5. The homogenization light path structure based on the fluorescence hyperspectral imaging system according to claim 4 is characterized in that the adjusting structure comprises a knob (201), a main shaft (202) and a secondary shaft (203), the knob (201) is fixed on the top of the main shaft (202), the main shaft (202) extends into a small hole formed in the body of the collimation light guide lens barrel (102), the secondary shaft (203) is connected to the side surface of the bottom of the main shaft (202), a transverse strip-shaped limiting opening is formed in the top of the first compound eye lens group (104) along the length direction of the top of the first compound eye lens group, and the bottom of the secondary shaft (203) is connected to the first compound eye lens group (104) in a sliding mode through the limiting opening.
6. The homogenization light path structure based on the fluorescence hyperspectral imaging system according to claim 1 is characterized in that an adjusting structure is arranged on the top of the focusing lens (106), and the adjusting structure is used for adjusting the distance between the focusing lens (106) and the second fly-eye lens group (105) in the horizontal direction.
7. The homogenization light path structure based on the fluorescence hyperspectral imaging system according to claim 6 is characterized in that the adjusting structure comprises a knob (201), a main shaft (202) and a secondary shaft (203), the knob (201) is fixed on the top of the main shaft (202), the main shaft (202) extends into a small hole formed on the body of the collimation light guide lens barrel (102), the secondary shaft (203) is connected to the side surface of the bottom of the main shaft (202), a transverse strip-shaped limiting opening is arranged on the top of the focusing lens (106) along the length direction of the focusing lens, and the bottom of the secondary shaft (203) is connected to the focusing lens (106) through the limiting opening in a sliding manner.
8. The homogenization light path structure based on the fluorescence hyperspectral imaging system according to claim 1 is characterized in that the light source (101) adopts a xenon lamp light source (101).
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| CN202210477777.1A CN114894309A (en) | 2022-04-29 | 2022-04-29 | Homogenization light path structure based on fluorescence hyperspectral imaging system |
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| CN202210477777.1A CN114894309A (en) | 2022-04-29 | 2022-04-29 | Homogenization light path structure based on fluorescence hyperspectral imaging system |
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