CN114886815B - Method for extracting purely natural allergy-relieving anti-aging component from centella asiatica - Google Patents
Method for extracting purely natural allergy-relieving anti-aging component from centella asiatica Download PDFInfo
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The application is applicable to the technical field of biological extraction, and provides an extraction method of purely natural allergy-free anti-aging components in centella asiatica, centella asiatica extract and application thereof, wherein the extraction method comprises the following steps: extracting herba Centellae powder with glycol dimethyl ether and ethanol solution; heating the obtained extract from normal temperature to 100-130 ℃ for 0.1-10 s, and transferring to normal temperature environment after heat preservation; diluting the obtained centella asiatica extract with water, adding a decoloring agent, and performing heat preservation decoloring treatment; concentrating the filtrate under reduced pressure with vacuum degree lower than-0.05 Mpa until the quality is constant to obtain herba Centellae extract. According to the method, the extraction liquid is subjected to rapid heating treatment from normal temperature, so that impurities affecting the yield of the total glycosides of centella asiatica can be rapidly vaporized and removed, the yield of the total glycosides of centella asiatica is greatly improved, and the obtained centella asiatica extract is subjected to thermal insulation decolorization and reduced pressure concentration treatment for a short time so as to further improve the yield and purity stability of the total glycosides of centella asiatica.
Description
Technical Field
The application belongs to the technical field of biological extraction, and particularly relates to an extraction method of purely natural allergy-relieving anti-aging components in centella asiatica.
Background
Centella asiatica is a dry whole herb of centella asiatica belonging to the family Umbelliferae, and also called Glechoma hederacea, which belongs to one of Chinese medicinal materials. It has good therapeutic effect on traumatic injury, heatstroke diarrhea, carbuncle, skin sore, etc., and has medicinal value, and can be used for cosmetic.
However, the pharmacology and the effect of centella asiatica are not separable from their active ingredients. The centella asiatica has complex chemical components, mainly contains centella asiatica total glycosides as active components, wherein the centella asiatica total glycosides are a mixture of glycosides formed by triterpenic acid, and comprise asiaticoside B, madecassoside and asiaticoside, and have the efficacy of soothing the nerves and resisting the aging; the centella asiatica chemical components also comprise asiatic acid, madecassic acid, asiaticoside, n-heptacosyl, 2,4, 6-tri-tert-butylbenzene, p-hydroxybenzoic acid, marigold, quercetin, hexacosanol octanoate, kaempferol, quercetin, carotene, vanillic acid, succinic acid, etc. The structure of the components is complex and similar, so that the extraction work of the centella asiatica extract is difficult, uneven extraction on the market is also an industry disorder, and the existing extraction method for the purely natural allergy-relieving and anti-aging components in centella asiatica generally has the problems of long extraction time, high production cost and lower yield of total glycosides of centella asiatica.
Disclosure of Invention
The embodiment of the application provides an extraction method of purely natural allergy-relieving anti-aging components in centella asiatica, and aims to solve the problems that the existing extraction method for purely natural allergy-relieving anti-aging components in centella asiatica is long in extraction time, high in production cost and low in total asiaticoside yield.
The embodiment of the application is realized in such a way that the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica comprises the following steps:
extracting herba Centellae powder with glycol dimethyl ether and ethanol solution to obtain extractive solution;
rapidly heating the extracting solution to 100-130 ℃ from normal temperature, wherein the heating time is 0.1-10 s, and transferring the extracting solution to a normal temperature environment after heat preservation for 1-3min to obtain centella asiatica extract;
diluting the centella asiatica extract with water, and then adding a decoloring agent for heat preservation and decoloring treatment;
filtering the decolorized solution, concentrating the filtrate under reduced pressure under vacuum degree lower than-0.09 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
The embodiment of the application also provides a centella asiatica extract, which is prepared by the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica.
The embodiment of the application also provides an centella asiatica extract prepared by the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica or application of the centella asiatica extract in the field of cosmetics.
According to the extraction method of the purely natural allergy-relieving anti-aging component in the centella asiatica provided by the embodiment of the application, centella asiatica powder is subjected to leaching treatment through a mixed solution of ethylene glycol dimethyl ether and ethanol, and the obtained extract is subjected to rapid heating treatment from normal temperature, so that impurities affecting the yield of total glycosides of centella asiatica can be rapidly vaporized and removed, the yield of total glycosides of centella asiatica can be greatly improved, and further the obtained centella asiatica extract is subjected to thermal insulation decolorization and reduced pressure concentration treatment for a short time so as to further improve the yield and purity stability of total glycosides of centella asiatica; in addition, the extraction process is simple, the time is short, the production cost is low, and the biological safety is high.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be described in further detail with reference to specific embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the present application.
In order to solve the problems that the existing extraction method for the purely natural allergy-free components in centella asiatica generally has long extraction time, high production cost and low yield of centella asiatica total glycosides, the embodiment of the application provides the extraction method for the purely natural allergy-free components in centella asiatica, which comprises the steps of leaching centella asiatica powder through a mixed solution of ethylene glycol dimethyl ether and ethanol, and rapidly heating the obtained extract from normal temperature, so that impurities affecting the yield of centella asiatica total glycosides can be rapidly vaporized and removed, the yield of centella asiatica total glycosides is greatly improved, and further the obtained centella asiatica extract is subjected to heat preservation and decolorization in a short time and reduced pressure concentration treatment to further improve the yield and purity stability of centella asiatica total glycosides; in addition, the extraction process is simple, the time is short, the production cost is low, and the biological safety is high.
Specifically, the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica provided by the embodiment of the application comprises the following steps:
step S1: extracting herba Centellae powder with glycol dimethyl ether and ethanol solution to obtain extractive solution.
In the embodiment of the application, the whole centella asiatica is crushed into powder and then passes through a 10-mesh sieve, the sieved centella asiatica powder is subjected to leaching treatment by using a mixed solution of ethylene glycol dimethyl ether and ethanol, wherein as for the extraction solution, one or any two of ethyl acetate, ethanol, ethylene glycol dimethyl ether, betaine and triethanolamine are respectively selected and mixed to be used as the extraction solution of centella asiatica powder in the early practical process, the mixed solution of ethylene glycol dimethyl ether and ethanol corresponds to the highest yield of the following centella asiatica total glycosides, and further experiments prove that the volume ratio of the ethylene glycol dimethyl ether to the ethanol solution is preferably 0.1 (2-4).
In the embodiment of the present application, the mass fraction of the ethanol solution is preferably 80-95%, and the higher the mass fraction, the better the requirement of the present application is, and more preferably 95%.
Step S2: and (3) rapidly heating the extracting solution to 100-130 ℃ from normal temperature, wherein the heating time is 0.1-10 s, and after heat preservation for 1-3min, displacing the extracting solution into a normal temperature environment to obtain centella asiatica extract.
In the embodiment of the application, the extraction liquid is quickly heated to be higher than the conventional boiling points of ethanol and ethylene glycol dimethyl ether, so that impurities affecting the yield of the total glycosides of centella asiatica can be quickly vaporized and removed, and the yield and purity of the total glycosides of centella asiatica are greatly improved.
Step S3: diluting the centella asiatica extract with water, and then adding a decoloring agent for heat preservation and decoloring treatment.
In the embodiment of the application, the decoloring agent is one or a mixture of more of active carbon, active clay and active silica gel. According to the experiment, more preferably, the decoloring agent is prepared by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1.
In the embodiment of the application, the thermal insulation decoloring treatment conditions are as follows: the temperature is 45-55 ℃ and the decoloring time is 15-25 min. More preferably, the incubation decolorization temperature is 50℃and the decolorization time is 20min. Wherein, the research of the application proves that the heat preservation decolorization temperature is not more than 55 ℃ and the decolorization time is not more than 25min, otherwise, the loss of the total glycosides of centella asiatica is large, and the yield is reduced; in addition, the temperature of thermal insulation and decolorization is not lower than 45 ℃, or the decolorization time is not lower than 15min, otherwise the decolorization effect is not good and the purity of the total asiaticoside is affected.
Step S4: filtering the decolorized solution, concentrating the filtrate under reduced pressure under vacuum degree lower than-0.05 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
In the examples of the present application, the degree of vacuum has little influence on the yield of the obtained asiaticoside, but has a certain influence on the purity of the asiaticoside, and the purity tends to decrease when the degree of vacuum is higher than-0.07 Mpa, and therefore, the degree of vacuum is preferably not higher than-0.07 Mpa; more preferably, the vacuum is-0.06 Mpa.
The embodiment of the application also provides a centella asiatica extract, which is prepared by the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica.
The embodiment of the application also provides an centella asiatica extract prepared by the extraction method of the purely natural allergy-relieving anti-aging component in centella asiatica or application of the centella asiatica extract in the field of cosmetics. The addition amount of the centella asiatica extract in the cosmetic is only 0.1-0.5%, namely, the centella asiatica extract can be effective in a low amount.
Examples of certain embodiments of the present application are given below, which are not intended to limit the scope of the present application.
In addition, it should be noted that the numerical values set forth in the following examples are as precise as possible, but those skilled in the art will understand that each numerical value should be construed as a divisor rather than an absolute precise numerical value due to measurement errors and experimental operation problems that cannot be avoided.
Example 1
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 2:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 100 ℃ in advance, quickly heating the extracting solution to 100 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 91.32% and the purity is 90.12%.
Example 2
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 2.5:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 105 ℃ in advance, quickly heating the extracting solution to 105 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 90.71% and the purity is 91.27%.
Example 3
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 2.5:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 100 ℃ in advance, quickly heating the extracting solution to 100 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 90.19% and the purity is 90.78%.
Example 4
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 4:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 130 ℃ in advance, quickly heating the extracting solution to 130 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
The yield of the centella asiatica total glycosides is 92.42% and the purity is 93.27% by HPLC analysis.
Example 5
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 3.5:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 125 ℃ in advance, quickly heating the extracting solution to 125 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 93.51% and the purity is 94.11%.
Example 6
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 4:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 125 ℃ in advance, quickly heating the extracting solution to 125 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 92.98% and the purity is 93.11%.
Example 7
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 3:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 120 ℃ in advance, quickly heating the extracting solution to 120 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 97.24% and the purity is 98.19%.
Example 8
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 3:0.1 to obtain an extraction solution, and leaching centella asiatica powder by using the extraction solution to obtain an extraction solution; taking out 1mL of each sample in the extracting solution, respectively placing the samples into a stainless steel dish, further placing the samples into an oil bath pot which is heated to 120 ℃ in advance, quickly heating the extracting solution to 120 ℃, quickly volatilizing components in the extracting solution within the time range of 0.1-10 s, preserving heat for 2min, and then displacing the samples into a normal-temperature environment to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 97.59% and the purity is 98.22%.
Comparative example 1
Mixing 95% ethanol and ethylene glycol dimethyl ether according to a volume ratio of 3:0.1 to obtain an extraction solution, carrying out reflux extraction on centella asiatica powder by using the extraction solution, filtering the extraction solution, concentrating to obtain a concentrated solution, carrying out ethanol elution treatment on the concentrated solution through a resin column, collecting ethanol eluent, and recovering ethanol to obtain centella asiatica extract; diluting the centella asiatica extract with water, adding a decoloring agent formed by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1, and carrying out heat preservation decoloring treatment, wherein the using amount of the decoloring agent is 0.5% of the mass of the centella asiatica extract, the heat preservation decoloring temperature is 50 ℃, and the decoloring time is 20min; filtering the decolorized solution, concentrating the filtrate under reduced pressure of-0.06 Mpa until the quality is constant, and drying to obtain herba Centellae extract.
HPLC analysis shows that the yield of asiaticoside is 81.27% and the purity is 73.43%.
In sum, according to the embodiment of the application, the centella asiatica powder is subjected to leaching treatment through the mixed solution of ethylene glycol dimethyl ether and ethanol, and the obtained extract is subjected to rapid heating treatment from normal temperature, so that impurities affecting the yield of centella asiatica total glycosides can be rapidly vaporized and removed, the yield of centella asiatica total glycosides can be greatly improved, and the obtained centella asiatica extract is subjected to thermal insulation decolorization and reduced pressure concentration treatment for a short time so as to further improve the yield and purity stability of centella asiatica total glycosides.
In addition, in comparative example 1, the extraction of centella asiatica extract is carried out by adopting the separate ethanol reflux extraction and resin column elution treatment means, and the yield and purity of the corresponding centella asiatica total glycosides are not as good as those of the examples, so that the embodiment of the application is beneficial to quickly vaporizing and removing impurities affecting the yield of centella asiatica total glycosides by quickly heating the extract to be higher than the conventional boiling points of ethanol and ethylene glycol dimethyl ether, thereby greatly improving the yield and purity of centella asiatica total glycosides.
In addition, biological safety tests show that the centella asiatica extract prepared in the embodiments 1-8 is low in biological irritation and high in safety; the skin care product is added into the skin care product with the addition amount of 0.1-0.5wt% to play a role in relieving skin itch and eliminating skin sensitive reaction, and further improve the anti-aging and allergy-relieving skin care effects of the skin care product.
The centella asiatica extract obtained in example 8 was added to a commercial skin care product (the main ingredients of the commercial skin care product are water, cyclopentadimethicone, butylene glycol, glycerin, ammonium acryloyldimethyl taurate, trehalose, sucrose, phenoxyethanol, dimethicone, sorbitol) at an addition amount of 0.3wt% to obtain a test product. 40 volunteers (sensitive skin, skin with clinical symptoms of itching and skin sensitivity) were selected, and the volunteers were randomly divided into two groups of 20, one group of the test products was smeared on the skin and the easy-to-develop part and the other group of the commercial skin care products was smeared after daily face cleaning at night. The efficacy was observed and counted after one month and is shown in table 1.
Efficacy evaluation criteria: cure (the clinical manifestation of the skin disappears and the skin sensitivity reaction does not exist within one month), is effective (the clinical manifestation of the skin is reduced and the skin sensitivity reaction is reduced), and is ineffective (the clinical manifestation of the skin is not improved).
TABLE 1
| Healing | Effective and effective | Invalidation of | |
| Test article | 18 | 2 | 0 |
| Commercially available skin care product | 10 | 7 | 3 |
In addition, in the early development process, related experimental researches are carried out on the extraction process of the centella asiatica extract, wherein the experimental scheme with more obvious influence is specifically shown in the following example.
(1) The same procedure as in example 8 was followed except that the vacuum degree used was varied (-0.05 MPa, -0.06MPa, -0.07MPa, -0.08 MPa), and the process conditions were unchanged.
TABLE 2
| Vacuum degree (MPa) | Yield of centella asiatica total glycosides (%) | Purity of centella asiatica total glycosides (%) | |
| Experiment 1 | -0.05 | 97.46 | 90.98 |
| Experiment 2 | -0.06 | 97.59 | 98.22 |
| Experiment 3 | -0.07 | 97.51 | 95.43 |
| Experiment 4 | -0.08 | 97.56 | 89.29 |
(2) The same procedure as in example 8 was followed except that the decolorization time was changed (10 min;20min;30 min), and the HPLC analysis results are shown in Table 3.
TABLE 3 Table 3
| Decoloring time (min) | Yield of centella asiatica total glycosides (%) | Purity of centella asiatica total glycosides (%) | |
| Experiment 1 | 10 | 90.43 | 84.75 |
| Experiment 2 | 20 | 97.59 | 98.22 |
| Experiment 3 | 30 | 88.14 | 92.36 |
In summary, it is apparent from tables 1 to 2 that the degree of vacuum has little effect on the yield of the obtained asiaticoside, but has a certain effect on the purity of the asiaticoside, and the purity tends to decrease when the degree of vacuum is higher than-0.07 Mpa; the excessive short decolorization time can directly affect the yield and purity of the obtained asiaticoside, and the possible reasons are that the excessive short decolorization time, insufficient decolorization effect, excessive long decolorization time and large loss of the asiaticoside lead to the reduction of the yield and purity.
The foregoing description of the preferred embodiments of the present application is not intended to be limiting, but is intended to cover any and all modifications, equivalents, and alternatives falling within the spirit and principles of the present application.
Claims (8)
1. A method for extracting purely natural allergy-relieving anti-aging components from centella asiatica is characterized by comprising the following steps:
extracting herba Centellae powder with glycol dimethyl ether and ethanol solution to obtain extractive solution;
rapidly heating the extracting solution to 100-130 ℃ from normal temperature, wherein the heating time is 0.1-10 s, and transferring the extracting solution to a normal temperature environment after heat preservation for 1-3min to obtain centella asiatica extract;
diluting the centella asiatica extract with water, and then adding a decoloring agent for heat preservation and decoloring treatment;
filtering the decolorized solution, concentrating the filtrate under reduced pressure under vacuum degree lower than-0.05 Mpa until the quality is constant, and drying to obtain herba Centellae extract;
the volume ratio of the ethylene glycol dimethyl ether to the ethanol solution is 0.1 (2-4);
the mass fraction of the ethanol solution is 80-95%.
2. The method for extracting pure natural allergy-relieving and anti-aging ingredients from centella asiatica according to claim 1, wherein the decolorizer is one or more of activated carbon, activated clay and activated silica gel.
3. The method for extracting pure natural allergy-relieving and anti-aging components from centella asiatica according to claim 1 or 2, wherein the decoloring agent is prepared by mixing active carbon, activated clay and active silica gel in a mass ratio of 1:1:1.
4. The method for extracting pure natural allergy-free components from centella asiatica according to claim 1, wherein the thermal-insulation decoloring treatment conditions are as follows: the temperature is 45-55 ℃ and the decoloring time is 15-25 min.
5. The method for extracting pure natural allergy-free components from centella asiatica according to claim 1 or 4, wherein the thermal-insulation decoloring treatment conditions are as follows: the temperature of thermal insulation and decolorization is 50 ℃ and the decolorization time is 20min.
6. The method for extracting pure natural anti-aging components from centella asiatica according to claim 1, wherein the vacuum is not higher than-0.07 Mpa.
7. An centella asiatica extract, characterized in that the centella asiatica extract is prepared by the method for extracting purely natural allergy-relieving and anti-aging components in centella asiatica according to any one of claims 1-6.
8. Use of the centella asiatica extract of claim 7 in the cosmetic field.
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| CN102532244A (en) * | 2011-12-31 | 2012-07-04 | 广西大学 | Method for preparing high-purity asiaticosid |
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