CN114875050A - Rice stem basal dwarfing control gene, protein, mutant gene, mutant and application thereof - Google Patents

Rice stem basal dwarfing control gene, protein, mutant gene, mutant and application thereof Download PDF

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CN114875050A
CN114875050A CN202210726272.4A CN202210726272A CN114875050A CN 114875050 A CN114875050 A CN 114875050A CN 202210726272 A CN202210726272 A CN 202210726272A CN 114875050 A CN114875050 A CN 114875050A
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张晓波
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Anhui Dingke Seed Industry Co ltd
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Abstract

The invention discloses an application of a rice stem basal dwarfing control gene and protein, wherein the gene is shown as Seq ID No: 1, and the application is as follows: (1) the application in regulating the height of rice plants; (2) the application in the lodging resistance of rice; (3) application in regulating and controlling rice yield. The invention also discloses a rice stem base dwarfing control mutant gene which is expressed in Seq I D No: 1, from 5269 th to 5279 th, and a gene lacking 11 nucleotides. Also discloses the application of the mutant gene and the biological material containing the mutant gene. In addition, the application of the rice stem base dwarfing control mutant is also disclosed, which is mainly applied in a crossbreeding mode. The dwarfing control gene, the protein, the mutant gene and the mutant of the basal part of the stalk can be used for reducing the plant height of rice and improving the lodging resistance and the yield of the rice. Particularly, the method can improve the rice with SD1 but still over high plant height, and has important application value in rice breeding.

Description

Rice stem basal dwarfing control gene, protein, mutant gene, mutant and application thereof
Technical Field
The invention relates to the field of plant genetic engineering, in particular to a rice stem basal dwarfing control gene, protein, mutant gene, mutant and application thereof.
Background
Rice is one of the most important food crops in the world. The cultivation of lodging-resistant rice is a powerful guarantee for realizing high and stable yield of rice, and the cultivation of short-stalk rice is an effective means for resisting lodging of rice, so that the rice dwarfing is proved to be an ideal way for solving the contradiction between high yield and lodging of rice. At present, the rice production mainly depends on a green revolution gene SD1 and each allele thereof, and the rice dwarf source is single. In addition, in rice practice, even if the hybrid rice contains the homozygous SD1 gene, the plant height of some hybrid rice combinations is too high, and the weight of the panicle is heavy, so that the rice is lodging and yield is reduced. Therefore, the excavation of new dwarf gene resources is still an important direction in the research of rice genetic breeding.
Disclosure of Invention
The invention aims to solve the technical problem of providing a novel rice stem dwarfing control gene, protein, mutant gene and mutant, and provides a novel idea and method for rice short-stalk breeding based on the development of related application.
In order to solve the technical problems, the invention adopts the following technical scheme:
in one aspect, the invention provides an application of a rice stem basal dwarfing control gene, wherein the gene is shown as Seq ID No: 1 or the cDNA sequence of the gene is shown as Seq ID No: 2 is shown in the specification; the application is as follows:
(1) the application in regulating the height of rice plants;
(2) the application in the lodging resistance of rice;
(3) application in regulating and controlling rice yield.
As a further improvement of the present invention, the gene is a negative regulatory gene.
Further, the plant height of the rice is reduced or the lodging resistance of the rice is improved or the yield of the rice is improved through gene knockout.
In another aspect, the invention provides an application of the rice stem base dwarfing control protein, wherein the protein is a protein represented by Seq ID No: 3;
the application is as follows:
(1) the application in regulating the height of rice plants;
(2) the application in the lodging resistance of rice;
(3) application in regulating and controlling rice yield.
In another aspect, the present invention provides a rice stem base dwarfing control mutant gene, wherein the mutant gene is represented by Seq ID No: 1, wherein 11 nucleotides from 5269 to 5279 of the gene sequence are deleted; or the cDNA sequence of said gene is as described in Seq ID No: 2, the 11-base deletion sequence at position 2509-2519 in the cDNA sequence shown in figure 2.
In still another aspect, the present invention provides a protein encoded by the above mutant gene, such as Seq ID No: 4.
In still another aspect, the present invention further provides an application of the mutant gene, protein, plasmid, plant expression vector or host cell, wherein the application is as follows:
(1) the application in reducing the height of rice plants;
(2) the application of the rice in improving the lodging resistance of rice;
(3) application in improving rice yield.
Further, the rice plants in (1), (2) and (3) are rice plants which have the SD1 gene and are still too tall.
In another aspect, the invention also provides an application of a rice stem base dwarfing control mutant, wherein the mutant contains the mutant gene, and the mutant is obtained by a cross breeding method:
(1) the plant height of the rice is reduced;
(2) the lodging resistance of the rice is improved;
(3) the rice yield is improved.
Further, the rice plants in (1), (2) and (3) are rice plants which have the SD1 gene and are still too tall.
The present invention clones one new gene LOC _ Os02g27620(dJH3017-Seq ID No: 1) for controlling rice stalk base dwarfing and encoding inositol polyphosphate 5-phosphatase (protein sequence shown in Seq ID No: 3). The mutant gene (d50 allele) of the invention is the gene obtained by radiating the high-stalk rice Jinghui 3017 (which already contains SD1) with cobalt 60 in Seq ID No: 1 by deletion of 11 bases in the gene sequence shown in the specification. The dwarfing control gene, the protein, the mutant gene and the mutant of the basal part of the stalk can be used for reducing the plant height of rice, realizing rice dwarfing breeding, improving the lodging resistance of the rice and improving the rice yield. The gene disclosed by the invention has an additive effect with SD1, and particularly can improve rice which has SD1 but still has too high plant height in Benzhou province, so that the plant height of the rice is reduced to a proper range, and the gene has an important application value in rice breeding.
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The foregoing is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description.
FIG. 1 is a comparison of the phenotypes of wild type and dwarf mutant vitellogenin;
FIG. 2 is a drawing showing the results of gene mapping by the BSA method;
FIG. 3 shows the sequencing alignment of wild type and short stalk mutant vitexin dwarf on dJH3017 gene.
Detailed Description
The following examples further illustrate the present invention but should not be construed as limiting the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
First, obtaining of rice stem base dwarfing control mutant
Vitex dwarf is a dwarf mutant obtained by cobalt 60 irradiation of vitex negundo 3017. Jinghui 3017 is a disease-resistant rice restorer with high combining ability, and it contains SD1, but its plant height is still very high, and it is about 1.5m in Hangzhou. The plant height of the hybrid combination matched with the strain is too high. The plant height of the dwarf mutant obtained by cobalt 60 radiation of Jinghui 3017 is about 1.0m (as shown in FIG. 1).
Second, preliminary gene mapping
To locate the gene controlling the dwarf phenotype of vitexin dwarf, the F2 population was obtained by crossing Morobekan with vitexin. Respectively extracting one DNA of a female parent sample, one DNA of a male parent sample, one DNA pool of 10F 2 short-stem plants and one DNA pool of 10F 2 high-stem plants, and then carrying out marker linkage Analysis according to a BSA method (a segregant group mixed Analysis method or a mixed group Analysis method, also called a bulk Segregation Analysis method). Polymorphism analysis was performed using 96 SSR markers evenly distributed on 12 chromosomes.
Partial results are shown in FIG. 2, the bottom of FIG. 2 is SSR marker name (RM omitted), each marker corresponds to maternal DNA, paternal DNA, short-stalk DNA pool and high-stalk DNA pool (from left to right) for PCR amplification, and non-denaturing PAGE analysis is performed. As is clear from the results shown in FIG. 2, the short stalk gene of vitexin dwarf is linked to RM71, RM324, RM5521 and RM475 located on the second chromosome.
Third, the gene is further positioned
And further selecting dwarf plants from the F2 population to extract DNA, and further positioning dwarf genes. The results of the discovery of the recessive dwarf phenotype and marker localization are shown in Table 1. The female parent vitexin dwarf marker band pattern is designated as 1, the male parent Morobekan band pattern is designated as 2, and the heterozygous band pattern is designated as 3. The dwarf gene is located between RM324 and D223A (table 1).
Table 1: recessive dwarf phenotype and marker location result table
Figure BDA0003711080910000041
Figure BDA0003711080910000051
Figure BDA0003711080910000061
Figure BDA0003711080910000071
Fourth, gene sequencing and cloning
Verification of the combination of Gene resequencing and first-generation DNA sequencing FIG. 3, the gene LOC _ Os02g27620(dJH3017) which controls the rice dwarf phenotype (gene which controls rice stem basal dwarfing) was cloned and encodes inositol polyphosphate 5-phosphatase (Seq ID No: 3).
The PCR primers used for the first-generation sequencing verification were (Seq ID No: 5-10):
d50-1 TTGCGCCAAGAAAGAGTGGGG
d50-2 AATGGCTGGTCATGGCGGAGT
d50-3 CCTTTAGCCATGCAGGAAAAGCAG
d50-4 TGCCTATATTAGGGAGCAGTGGG
d50-5 CACACCGAAACCCAGTAGAAAATTTGG
d50-6 CGTTGGCATTAGTAAAGGCAGACTCC
only the primer pair d50-3 and d50-4 detected the mutation site, and proved that the mutant had 11 bases deleted after being irradiated.
dJH3017 recessive homozygous individual was selected, d50-3 and d50-4 were subjected to PCR, and d50-3 was used as a sequencing primer. As shown in fig. 3, the sequencing alignment of wild-type and dwarf mutant vitellogenin on the dJH3017 gene shows that the mutant gene is identified in Seq ID No: 1, 11 nucleotides are deleted from 5269 to 5279.
dJH3017 and d12, d32 and d50 are allelic, but dJH3017 mutation site is closer to gene rear part, and dwarfing symptom is moderate and more suitable for breeding.
Fifth, application of mutant gene and mutant
The mutant gene is used for improving Basmati (Basmati, Basmati rice and Indian scented rice) so as to reduce the plant height.
Using the hybridization of vitexin dwarf and Basmati, planting in Hainan Ling water in F2 generation, measuring the ear length and plant height in the yellow maturity period of the seeds. A total of 140 rows 6 strains/row were measured. Selecting 25 plants with the plant height of less than 75cm and 25 plants with the plant height of more than 122 cm. The newly developed CAPS marker identified that 25 dwarf F2 were homozygous dJH3017 using 11 deletion bases. As shown in Table 2, the 25 short stems F2 have obvious dwarfing compared with the high stem F2, the spike length does not change greatly, and the good agronomic characters are maintained. The dJH3017 shows that it can be used for improving the plant height of paddy rice in different backgrounds and has little influence on the ear length, thus improving the lodging resistance and increasing the yield.
TABLE 2 plant height and ear length measuring meter
Figure BDA0003711080910000081
Figure BDA0003711080910000091
Note: the A series is short stem plants; b series is high-stem plant
From the application, the invention utilizes 11 base deletions on LOC _ Os02g27620 and utilizes the deletions to introduce other rice materials to generate a plant dwarfing phenotype, and can be directly utilized in breeding.
Sixth, other applications
The dJH3017 gene cloned in the invention proves that the gene has the function of controlling dwarfing of the base part of the rice stem, so the gene can be directly applied to rice dwarfing breeding, lodging-resistant breeding and high-yield breeding. The gene is a negative regulatory gene, and the breeding purpose can be realized by means of gene knockout and the like. The mutant gene and various biological materials containing the mutant gene can be directly utilized to carry out rice breeding improvement in a transgenic way. In addition, mutants containing the mutant genes can be directly adopted to be hybridized with other rice materials to improve rice breeding, particularly, the genes have an additive effect with SD1, particularly, the rice which has SD1 but still has too high plant height in the province can be improved, the plant height of the rice is reduced to a proper range, and the mutant genes have important application value in rice breeding.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention in any way, and it will be apparent to those skilled in the art that the above description of the present invention can be applied to various modifications, equivalent variations or modifications without departing from the spirit and scope of the present invention.
Sequence listing
<110> institute of Rice research in China
<120> rice stem basal dwarfing control gene, protein, mutant gene, mutant and application thereof
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ttcttcctct ccgcctccat ctcctcccct tccccttcca cctccactcc ccctccccct 180
tcccccatcc cctccacctc cccgcgcccc accctccgct cctcctccct cccgcccgcc 240
tccaccccct ccccctcctc ctctgcctcc tcctcctctg gctccctcca ccacctccac 300
cccacccact ccctcccggc cttctccgcc gcggccgcgg cggcggcagc cgacgcctgg 360
cccccgccac cgggggcgca ccactccggc tccctccagg agttcgccgc ccccgcgtcc 420
tcctccgcga cccgcccgcc cccgcgcgcc gccgtgcgcg ccgaccgccc gccgccgctc 480
gacctgcgcc cgcgcccgcc cagggaatcc caggctggcg ccgcgctccg cgcgatcgcc 540
gtggacgggg ccaccggctc gcacctgtgg gccgtcggcg acgccggcgt cagggtgtgg 600
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aagaatatgg gccggttaca tggatggtag tattcagctg ttggacttgg aaggtaactt 2640
actaggaggc tggatcgcac atagcagtcc agttctgagt atggctgttg gaggttcata 2700
catctttaca atggctggtc atggcggagt ccgtggatgg aatttgtcat ctccagggcc 2760
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gcacttagtc atctgctact attatacaga ccaaatccta gtaacactga acaagagtgt 3120
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ccccatattc atttggtagc agttgttgtg caaaaccaat aaatcattgt taccaagatg 4380
atatctggtg gtttgtgcat acaattaatg aatgagaggt gtgcaatact gatcagaaaa 4440
tgaaaaaaaa aagacaaatt ttaaaacaca ttatgggcct aaaatgtaac cacccactag 4500
ttctgttatt tttctggggg agtacagata ttacttcaaa aggactgtat tctgtcttat 4560
ctgctgcttt gttcatgtat gcctatatta gggagcagtg ggattgagga tgagaataca 4620
tgataggagt atttgctttg taaattgcca ttttgctgct catatggaag ctgtgagtcg 4680
acggaatgaa gattttgacc atgtctttag aacaatgacc tttgccaccc cttcgagtgg 4740
aataatgaca acatcaggtg tttgttcaac cttttcccat tatttttcag tattttccaa 4800
tgggcattca agtcaaatga cctgtaaatt tgttgtgata ttacaatcag caatgagcaa 4860
atcttataca ctgttacttg ttttggtggt cttgcagttt ctagttctac tggccagctt 4920
cttcgaggag caaatgtatg gtgtattaat gttggttcat tacattttct cggtggatag 4980
ggagtagatt ctcatcttga tatactaagt tgttcctatt ataatttaca gggatcaaga 5040
atgcctgagt tgtcagacac ggacatgatt gtctttcttg gtgacttcaa ttaccgcctt 5100
tatgatattt cctatgatga tgcaatgggc ttagtttccc ggagatgctt tgactggcta 5160
aaaaataatg accaactgcg agcagaaatg agatctggga gagtcttcca gggactacgt 5220
gaaggggatt tcaagtttcc ccctacatac aaatttgaga aacatacagc aggcttatca 5280
ggtatcttac tttgtttttt attcgtggaa tcccaaagta catataaaga tcatcacctt 5340
tatttgtcat atagggtatg atagcagtga gaagaggcgc attcctgcct ggtgtgacag 5400
aatcctatat cgtgatagcc gagttagttc agggaatgag tgttccttgg attgtcctgt 5460
ggtttcttca atatcactgt aagttatttg ttgtgcatgt ttttttttaa cccgaaaaag 5520
cttaaaatat gtcaatacct tgttattatg ttttccaggt atgactcttg catggaagca 5580
acagatagtg atcacaaacc tataaaatct gtgttcaatt tggatattgc ttatgttgac 5640
aaacagacaa tgaggcagaa atatgtggag ctaatgagct caaataataa agtggtgcat 5700
ttgcttcagg aacttgaagc attccctgga gtaaatataa ataattctaa catcatcttg 5760
caagatcgga atccatctgt tgtgaaattg caaaacagaa cagaagtcat cgcttgtttt 5820
gagatcattg gacaagcacc aaatttgtcc agcacacatt tctctgcttt tcctgcatgg 5880
ctaaaggtga gtcaatgctt tgtctgactc cttttactgt atttttatta aaataatcta 5940
atactagcaa cgtacaaaaa ctttgcacga ccgaatttct catgttttac atgggcacaa 6000
ctgcagagta gtagaaatat tagtctcttc agtatcaaga gttatgacaa aagagagcat 6060
atggtttcag ttatactccc tccgtttcag gttataagac gttttgactt tggtcaaagt 6120
caaactactt caaatttgat taagtttata gacaaatata gtaatattta taatactaaa 6180
ttagtttatt aaatcaataa ttgaatatat ttttataata aatttgtctt gggttgaaaa 6240
tgttattatt gttttctaca aacttagtca aacttgaagt aatttgactt tgaccaaagt 6300
caaaaacatc ttataacctg aaacggaggt agtagtagat tgacgcgttg gcattagtaa 6360
aggcagactc cagaatttgt tattacttgt tttgcttgtt ttattcttca ccattattaa 6420
ttcactgttg tgagcttaat gttttgaaaa ctgtgggcat atattcaata taggtctctc 6480
cagcagtcgg cataatatct ccgggacaga cggtagaggt cactttgcag cacagagacc 6540
tgcatagcca acaaaactat aatggaactt cattggatat tttgcctggt ggagctaccc 6600
aacaaaaggc agcaactgtt tttgcgaaaa taactggagt atattcaaca gttgcaaaat 6660
attacgaaat acatgtacaa caccagaact gcaggagcac attgccatcg agaggttata 6720
acttaggtga ccggtttttt taattttgag tttggtatca ggaatttagg aaatgtatgt 6780
tgtatcaaat gttctttttg agtgaagtca caaaatcaag gctttctact accctcaaca 6840
tgtgtatttt caagagagga aaattgtaaa cagtgtagca tacaacagcc caaattttct 6900
actgggtttc ggtgtggaa 6919
<210> 2
<211> 3243
<212> DNA
<213> Oryza sativa (Oryza sativa)
<400> 2
atggcggatc ccggcgactc cgccgcgtcc ccctccccgt gggacgacct ccccgacgac 60
ttcttcctct ccgcctccat ctcctcccct tccccttcca cctccactcc ccctccccct 120
tcccccatcc cctccacctc cccgcgcccc accctccgct cctcctccct cccgcccgcc 180
tccaccccct ccccctcctc ctctgcctcc tcctcctctg gctccctcca ccacctccac 240
cccacccact ccctcccggc cttctccgcc gcggccgcgg cggcggcagc cgacgcctgg 300
cccccgccac cgggggcgca ccactccggc tccctccagg agttcgccgc ccccgcgtcc 360
tcctccgcga cccgcccgcc cccgcgcgcc gccgtgcgcg ccgaccgccc gccgccgctc 420
gacctgcgcc cgcgcccgcc cagggaatcc caggctggcg ccgcgctccg cgcgatcgcc 480
gtggacgggg ccaccggctc gcacctgtgg gccgtcggcg acgccggcgt cagggtgtgg 540
agcctcgccg acgccttccg cgcgcccgcg tctcggcaga ggtggggcga cgaggctgcc 600
gcgccgttcc gcgagtcgcg caggacgcag ccggcgctgt gcctcgtggc tgacccttgc 660
cgcggcgttg tgtggagcgg ccacgccaat ggctggatca tggggtggag cgccgatcca 720
ggaggactag aggccgggga gtgcattgcc tgggaagctc accgtggacc agtattcgca 780
ttgaccacct ctctatatgg tgatttgtgg tctggctctg aagggggagt aatcaaagtt 840
tggtatgaag aaggaattga gaagtccctt tctttacaaa gagaagaaaa gcgtaagacc 900
tcctttttag ttgaaagatc attcattgac cttagagcca tggtcagtga tggaggggcc 960
tgtcccttgc ctgccgtaga tgtaaaactt ctgttgtctg acaattctag atcaaaagta 1020
tggagtgctg gttacctctc acttgcactc tgggattctt gcaccaagga gcttctgaaa 1080
gtgattagcg tggatggcca agtcgatact cgttttgata tcttatcttc tcaggatcca 1140
tttggctatg aaacaaagca aaaccttttc tctgctccaa ggaaagataa agctcgcagt 1200
cctgttggat ttttccagag atcacgaaat gctttaatgg gagcggctga tgcagtccgg 1260
agagttgctg ctaaagcagg atttggagat gattctcaaa gaatagaagc attagcaatg 1320
tcaattgatg ggatgatctg gacagggtct gcaaatggat gtcttgctcg atgggatggc 1380
aacggtaacc gtttgcaaga gtttcagcat catttgtgtt ctgttcaaag cattttcagc 1440
tttgggacaa gaatatgggc cggttacatg gatggtagta ttcagctgtt ggacttggaa 1500
ggtaacttac taggaggctg gatcgcacat agcagtccag ttctgagtat ggctgttgga 1560
ggttcataca tctttacaat ggctggtcat ggcggagtcc gtggatggaa tttgtcatct 1620
ccagggccta ttgacaacat tatgcgttct actttgattg aggctgagcc attatacaaa 1680
caatttgaat acatgaaagt gttggtgggt tcttggaatg tcgggcaaga aaaggcatct 1740
tatgagtcac taagagcttg gctaaagtta ccgacaccag aggttgggtt agtggtagtt 1800
ggattgcagg aggtggacat gggtgctggt tttcttgcaa tgtctgcagc taaagaaaca 1860
gttgggctag agggaagccc aaacggagat tggtggttgg atgcaattgg gcagcagtta 1920
aagggttact cttttgagcg tgttggctcg aggcagatgg ctggattgct tatctgtgta 1980
tgggtcagaa cacatcttaa gcagttcatt ggtgatattg ataatgctgc ggtagcatgt 2040
ggattagggc gagcaatcgg caacaaggga gcagtgggat tgaggatgag aatacatgat 2100
aggagtattt gctttgtaaa ttgccatttt gctgctcata tggaagctgt gagtcgacgg 2160
aatgaagatt ttgaccatgt ctttagaaca atgacctttg ccaccccttc gagtggaata 2220
atgacaacat cagtttctag ttctactggc cagcttcttc gaggagcaaa tggatcaaga 2280
atgcctgagt tgtcagacac ggacatgatt gtctttcttg gtgacttcaa ttaccgcctt 2340
tatgatattt cctatgatga tgcaatgggc ttagtttccc ggagatgctt tgactggcta 2400
aaaaataatg accaactgcg agcagaaatg agatctggga gagtcttcca gggactacgt 2460
gaaggggatt tcaagtttcc ccctacatac aaatttgaga aacatacagc aggcttatca 2520
gggtatgata gcagtgagaa gaggcgcatt cctgcctggt gtgacagaat cctatatcgt 2580
gatagccgag ttagttcagg gaatgagtgt tccttggatt gtcctgtggt ttcttcaata 2640
tcactgtatg actcttgcat ggaagcaaca gatagtgatc acaaacctat aaaatctgtg 2700
ttcaatttgg atattgctta tgttgacaaa cagacaatga ggcagaaata tgtggagcta 2760
atgagctcaa ataataaagt ggtgcatttg cttcaggaac ttgaagcatt ccctggagta 2820
aatataaata attctaacat catcttgcaa gatcggaatc catctgttgt gaaattgcaa 2880
aacagaacag aagtcatcgc ttgttttgag atcattggac aagcaccaaa tttgtccagc 2940
acacatttct ctgcttttcc tgcatggcta aaggtctctc cagcagtcgg cataatatct 3000
ccgggacaga cggtagaggt cactttgcag cacagagacc tgcatagcca acaaaactat 3060
aatggaactt cattggatat tttgcctggt ggagctaccc aacaaaaggc agcaactgtt 3120
tttgcgaaaa taactggagt atattcaaca gttgcaaaat attacgaaat acatgtacaa 3180
caccagaact gcaggagcac attgccatcg agaggttata acttaggtga ccggtttttt 3240
taa 3243
<210> 3
<211> 1080
<212> PRT
<213> Oryza sativa (Oryza sativa)
<400> 3
Met Ala Asp Pro Gly Asp Ser Ala Ala Ser Pro Ser Pro Trp Asp Asp
1 5 10 15
Leu Pro Asp Asp Phe Phe Leu Ser Ala Ser Ile Ser Ser Pro Ser Pro
20 25 30
Ser Thr Ser Thr Pro Pro Pro Pro Ser Pro Ile Pro Ser Thr Ser Pro
35 40 45
Arg Pro Thr Leu Arg Ser Ser Ser Leu Pro Pro Ala Ser Thr Pro Ser
50 55 60
Pro Ser Ser Ser Ala Ser Ser Ser Ser Gly Ser Leu His His Leu His
65 70 75 80
Pro Thr His Ser Leu Pro Ala Phe Ser Ala Ala Ala Ala Ala Ala Ala
85 90 95
Ala Asp Ala Trp Pro Pro Pro Pro Gly Ala His His Ser Gly Ser Leu
100 105 110
Gln Glu Phe Ala Ala Pro Ala Ser Ser Ser Ala Thr Arg Pro Pro Pro
115 120 125
Arg Ala Ala Val Arg Ala Asp Arg Pro Pro Pro Leu Asp Leu Arg Pro
130 135 140
Arg Pro Pro Arg Glu Ser Gln Ala Gly Ala Ala Leu Arg Ala Ile Ala
145 150 155 160
Val Asp Gly Ala Thr Gly Ser His Leu Trp Ala Val Gly Asp Ala Gly
165 170 175
Val Arg Val Trp Ser Leu Ala Asp Ala Phe Arg Ala Pro Ala Ser Arg
180 185 190
Gln Arg Trp Gly Asp Glu Ala Ala Ala Pro Phe Arg Glu Ser Arg Arg
195 200 205
Thr Gln Pro Ala Leu Cys Leu Val Ala Asp Pro Cys Arg Gly Val Val
210 215 220
Trp Ser Gly His Ala Asn Gly Trp Ile Met Gly Trp Ser Ala Asp Pro
225 230 235 240
Gly Gly Leu Glu Ala Gly Glu Cys Ile Ala Trp Glu Ala His Arg Gly
245 250 255
Pro Val Phe Ala Leu Thr Thr Ser Leu Tyr Gly Asp Leu Trp Ser Gly
260 265 270
Ser Glu Gly Gly Val Ile Lys Val Trp Tyr Glu Glu Gly Ile Glu Lys
275 280 285
Ser Leu Ser Leu Gln Arg Glu Glu Lys Arg Lys Thr Ser Phe Leu Val
290 295 300
Glu Arg Ser Phe Ile Asp Leu Arg Ala Met Val Ser Asp Gly Gly Ala
305 310 315 320
Cys Pro Leu Pro Ala Val Asp Val Lys Leu Leu Leu Ser Asp Asn Ser
325 330 335
Arg Ser Lys Val Trp Ser Ala Gly Tyr Leu Ser Leu Ala Leu Trp Asp
340 345 350
Ser Cys Thr Lys Glu Leu Leu Lys Val Ile Ser Val Asp Gly Gln Val
355 360 365
Asp Thr Arg Phe Asp Ile Leu Ser Ser Gln Asp Pro Phe Gly Tyr Glu
370 375 380
Thr Lys Gln Asn Leu Phe Ser Ala Pro Arg Lys Asp Lys Ala Arg Ser
385 390 395 400
Pro Val Gly Phe Phe Gln Arg Ser Arg Asn Ala Leu Met Gly Ala Ala
405 410 415
Asp Ala Val Arg Arg Val Ala Ala Lys Ala Gly Phe Gly Asp Asp Ser
420 425 430
Gln Arg Ile Glu Ala Leu Ala Met Ser Ile Asp Gly Met Ile Trp Thr
435 440 445
Gly Ser Ala Asn Gly Cys Leu Ala Arg Trp Asp Gly Asn Gly Asn Arg
450 455 460
Leu Gln Glu Phe Gln His His Leu Cys Ser Val Gln Ser Ile Phe Ser
465 470 475 480
Phe Gly Thr Arg Ile Trp Ala Gly Tyr Met Asp Gly Ser Ile Gln Leu
485 490 495
Leu Asp Leu Glu Gly Asn Leu Leu Gly Gly Trp Ile Ala His Ser Ser
500 505 510
Pro Val Leu Ser Met Ala Val Gly Gly Ser Tyr Ile Phe Thr Met Ala
515 520 525
Gly His Gly Gly Val Arg Gly Trp Asn Leu Ser Ser Pro Gly Pro Ile
530 535 540
Asp Asn Ile Met Arg Ser Thr Leu Ile Glu Ala Glu Pro Leu Tyr Lys
545 550 555 560
Gln Phe Glu Tyr Met Lys Val Leu Val Gly Ser Trp Asn Val Gly Gln
565 570 575
Glu Lys Ala Ser Tyr Glu Ser Leu Arg Ala Trp Leu Lys Leu Pro Thr
580 585 590
Pro Glu Val Gly Leu Val Val Val Gly Leu Gln Glu Val Asp Met Gly
595 600 605
Ala Gly Phe Leu Ala Met Ser Ala Ala Lys Glu Thr Val Gly Leu Glu
610 615 620
Gly Ser Pro Asn Gly Asp Trp Trp Leu Asp Ala Ile Gly Gln Gln Leu
625 630 635 640
Lys Gly Tyr Ser Phe Glu Arg Val Gly Ser Arg Gln Met Ala Gly Leu
645 650 655
Leu Ile Cys Val Trp Val Arg Thr His Leu Lys Gln Phe Ile Gly Asp
660 665 670
Ile Asp Asn Ala Ala Val Ala Cys Gly Leu Gly Arg Ala Ile Gly Asn
675 680 685
Lys Gly Ala Val Gly Leu Arg Met Arg Ile His Asp Arg Ser Ile Cys
690 695 700
Phe Val Asn Cys His Phe Ala Ala His Met Glu Ala Val Ser Arg Arg
705 710 715 720
Asn Glu Asp Phe Asp His Val Phe Arg Thr Met Thr Phe Ala Thr Pro
725 730 735
Ser Ser Gly Ile Met Thr Thr Ser Val Ser Ser Ser Thr Gly Gln Leu
740 745 750
Leu Arg Gly Ala Asn Gly Ser Arg Met Pro Glu Leu Ser Asp Thr Asp
755 760 765
Met Ile Val Phe Leu Gly Asp Phe Asn Tyr Arg Leu Tyr Asp Ile Ser
770 775 780
Tyr Asp Asp Ala Met Gly Leu Val Ser Arg Arg Cys Phe Asp Trp Leu
785 790 795 800
Lys Asn Asn Asp Gln Leu Arg Ala Glu Met Arg Ser Gly Arg Val Phe
805 810 815
Gln Gly Leu Arg Glu Gly Asp Phe Lys Phe Pro Pro Thr Tyr Lys Phe
820 825 830
Glu Lys His Thr Ala Gly Leu Ser Gly Tyr Asp Ser Ser Glu Lys Arg
835 840 845
Arg Ile Pro Ala Trp Cys Asp Arg Ile Leu Tyr Arg Asp Ser Arg Val
850 855 860
Ser Ser Gly Asn Glu Cys Ser Leu Asp Cys Pro Val Val Ser Ser Ile
865 870 875 880
Ser Leu Tyr Asp Ser Cys Met Glu Ala Thr Asp Ser Asp His Lys Pro
885 890 895
Ile Lys Ser Val Phe Asn Leu Asp Ile Ala Tyr Val Asp Lys Gln Thr
900 905 910
Met Arg Gln Lys Tyr Val Glu Leu Met Ser Ser Asn Asn Lys Val Val
915 920 925
His Leu Leu Gln Glu Leu Glu Ala Phe Pro Gly Val Asn Ile Asn Asn
930 935 940
Ser Asn Ile Ile Leu Gln Asp Arg Asn Pro Ser Val Val Lys Leu Gln
945 950 955 960
Asn Arg Thr Glu Val Ile Ala Cys Phe Glu Ile Ile Gly Gln Ala Pro
965 970 975
Asn Leu Ser Ser Thr His Phe Ser Ala Phe Pro Ala Trp Leu Lys Val
980 985 990
Ser Pro Ala Val Gly Ile Ile Ser Pro Gly Gln Thr Val Glu Val Thr
995 1000 1005
Leu Gln His Arg Asp Leu His Ser Gln Gln Asn Tyr Asn Gly Thr Ser
1010 1015 1020
Leu Asp Ile Leu Pro Gly Gly Ala Thr Gln Gln Lys Ala Ala Thr Val
1025 1030 1035 1040
Phe Ala Lys Ile Thr Gly Val Tyr Ser Thr Val Ala Lys Tyr Tyr Glu
1045 1050 1055
Ile His Val Gln His Gln Asn Cys Arg Ser Thr Leu Pro Ser Arg Gly
1060 1065 1070
Tyr Asn Leu Gly Asp Arg Phe Phe
1075 1080
<210> 4
<211> 893
<212> PRT
<213> Oryza sativa (Oryza sativa)
<400> 4
Met Ala Asp Pro Gly Asp Ser Ala Ala Ser Pro Ser Pro Trp Asp Asp
1 5 10 15
Leu Pro Asp Asp Phe Phe Leu Ser Ala Ser Ile Ser Ser Pro Ser Pro
20 25 30
Ser Thr Ser Thr Pro Pro Pro Pro Ser Pro Ile Pro Ser Thr Ser Pro
35 40 45
Arg Pro Thr Leu Arg Ser Ser Ser Leu Pro Pro Ala Ser Thr Pro Ser
50 55 60
Pro Ser Ser Ser Ala Ser Ser Ser Ser Gly Ser Leu His His Leu His
65 70 75 80
Pro Thr His Ser Leu Pro Ala Phe Ser Ala Ala Ala Ala Ala Ala Ala
85 90 95
Ala Asp Ala Trp Pro Pro Pro Pro Gly Ala His His Ser Gly Ser Leu
100 105 110
Gln Glu Phe Ala Ala Pro Ala Ser Ser Ser Ala Thr Arg Pro Pro Pro
115 120 125
Arg Ala Ala Val Arg Ala Asp Arg Pro Pro Pro Leu Asp Leu Arg Pro
130 135 140
Arg Pro Pro Arg Glu Ser Gln Ala Gly Ala Ala Leu Arg Ala Ile Ala
145 150 155 160
Val Asp Gly Ala Thr Gly Ser His Leu Trp Ala Val Gly Asp Ala Gly
165 170 175
Val Arg Val Trp Ser Leu Ala Asp Ala Phe Arg Ala Pro Ala Ser Arg
180 185 190
Gln Arg Trp Gly Asp Glu Ala Ala Ala Pro Phe Arg Glu Ser Arg Arg
195 200 205
Thr Gln Pro Ala Leu Cys Leu Val Ala Asp Pro Cys Arg Gly Val Val
210 215 220
Trp Ser Gly His Ala Asn Gly Trp Ile Met Gly Trp Ser Ala Asp Pro
225 230 235 240
Gly Gly Leu Glu Ala Gly Glu Cys Ile Ala Trp Glu Ala His Arg Gly
245 250 255
Pro Val Phe Ala Leu Thr Thr Ser Leu Tyr Gly Asp Leu Trp Ser Gly
260 265 270
Ser Glu Gly Gly Val Ile Lys Val Trp Tyr Glu Glu Gly Ile Glu Lys
275 280 285
Ser Leu Ser Leu Gln Arg Glu Glu Lys Arg Lys Thr Ser Phe Leu Val
290 295 300
Glu Arg Ser Phe Ile Asp Leu Arg Ala Met Val Ser Asp Gly Gly Ala
305 310 315 320
Cys Pro Leu Pro Ala Val Asp Val Lys Leu Leu Leu Ser Asp Asn Ser
325 330 335
Arg Ser Lys Val Trp Ser Ala Gly Tyr Leu Ser Leu Ala Leu Trp Asp
340 345 350
Ser Cys Thr Lys Glu Leu Leu Lys Val Ile Ser Val Asp Gly Gln Val
355 360 365
Asp Thr Arg Phe Asp Ile Leu Ser Ser Gln Asp Pro Phe Gly Tyr Glu
370 375 380
Thr Lys Gln Asn Leu Phe Ser Ala Pro Arg Lys Asp Lys Ala Arg Ser
385 390 395 400
Pro Val Gly Phe Phe Gln Arg Ser Arg Asn Ala Leu Met Gly Ala Ala
405 410 415
Asp Ala Val Arg Arg Val Ala Ala Lys Ala Gly Phe Gly Asp Asp Ser
420 425 430
Gln Arg Ile Glu Ala Leu Ala Met Ser Ile Asp Gly Met Ile Trp Thr
435 440 445
Gly Ser Ala Asn Gly Cys Leu Ala Arg Trp Asp Gly Asn Gly Asn Arg
450 455 460
Leu Gln Glu Phe Gln His His Leu Cys Ser Val Gln Ser Ile Phe Ser
465 470 475 480
Phe Gly Thr Arg Ile Trp Ala Gly Tyr Met Asp Gly Ser Ile Gln Leu
485 490 495
Leu Asp Leu Glu Gly Asn Leu Leu Gly Gly Trp Ile Ala His Ser Ser
500 505 510
Pro Val Leu Ser Met Ala Val Gly Gly Ser Tyr Ile Phe Thr Met Ala
515 520 525
Gly His Gly Gly Val Arg Gly Trp Asn Leu Ser Ser Pro Gly Pro Ile
530 535 540
Asp Asn Ile Met Arg Ser Thr Leu Ile Glu Ala Glu Pro Leu Tyr Lys
545 550 555 560
Gln Phe Glu Tyr Met Lys Val Leu Val Gly Ser Trp Asn Val Gly Gln
565 570 575
Glu Lys Ala Ser Tyr Glu Ser Leu Arg Ala Trp Leu Lys Leu Pro Thr
580 585 590
Pro Glu Val Gly Leu Val Val Val Gly Leu Gln Glu Val Asp Met Gly
595 600 605
Ala Gly Phe Leu Ala Met Ser Ala Ala Lys Glu Thr Val Gly Leu Glu
610 615 620
Gly Ser Pro Asn Gly Asp Trp Trp Leu Asp Ala Ile Gly Gln Gln Leu
625 630 635 640
Lys Gly Tyr Ser Phe Glu Arg Val Gly Ser Arg Gln Met Ala Gly Leu
645 650 655
Leu Ile Cys Val Trp Val Arg Thr His Leu Lys Gln Phe Ile Gly Asp
660 665 670
Ile Asp Asn Ala Ala Val Ala Cys Gly Leu Gly Arg Ala Ile Gly Asn
675 680 685
Lys Gly Ala Val Gly Leu Arg Met Arg Ile His Asp Arg Ser Ile Cys
690 695 700
Phe Val Asn Cys His Phe Ala Ala His Met Glu Ala Val Ser Arg Arg
705 710 715 720
Asn Glu Asp Phe Asp His Val Phe Arg Thr Met Thr Phe Ala Thr Pro
725 730 735
Ser Ser Gly Ile Met Thr Thr Ser Val Ser Ser Ser Thr Gly Gln Leu
740 745 750
Leu Arg Gly Ala Asn Gly Ser Arg Met Pro Glu Leu Ser Asp Thr Asp
755 760 765
Met Ile Val Phe Leu Gly Asp Phe Asn Tyr Arg Leu Tyr Asp Ile Ser
770 775 780
Tyr Asp Asp Ala Met Gly Leu Val Ser Arg Arg Cys Phe Asp Trp Leu
785 790 795 800
Lys Asn Asn Asp Gln Leu Arg Ala Glu Met Arg Ser Gly Arg Val Phe
805 810 815
Gln Gly Leu Arg Glu Gly Asp Phe Lys Phe Pro Pro Thr Tyr Lys Phe
820 825 830
Glu Lys His Thr Gln Gly Met Ile Ala Val Arg Arg Gly Ala Phe Leu
835 840 845
Pro Gly Val Thr Glu Ser Tyr Ile Val Ile Ala Glu Leu Val Gln Gly
850 855 860
Met Ser Val Pro Trp Ile Val Leu Trp Phe Leu Gln Tyr His Cys Met
865 870 875 880
Thr Leu Ala Trp Lys Gln Gln Ile Val Ile Thr Asn Leu
885 890
<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
ttgcgccaag aaagagtggg g 21
<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
aatggctggt catggcggag t 21
<210> 7
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
cctttagcca tgcaggaaaa gcag 24
<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
tgcctatatt agggagcagt ggg 23
<210> 9
<211> 27
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
cacaccgaaa cccagtagaa aatttgg 27
<210> 10
<211> 26
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
cgttggcatt agtaaaggca gactcc 26

Claims (10)

1. The application of a rice stem base dwarfing control gene is characterized in that the gene is shown as Seq ID No: 1 or the cDNA sequence of the gene is shown as Seq ID No: 2 is shown in the specification;
the application is as follows:
(1) the application in regulating the height of rice plants;
(2) the application in the lodging resistance of rice;
(3) application in regulating and controlling rice yield.
2. The use of claim 1, wherein the gene is a negative regulatory gene.
3. The use according to claim 1, wherein the plant height of rice is reduced or the lodging resistance of rice is increased or the yield of rice is increased by gene knockout.
4. The application of the rice stem base dwarfing control protein is characterized in that the protein is shown as Seq ID No: 3;
the application is as follows:
(1) the application in regulating the height of rice plants;
(2) the application in the lodging resistance of rice;
(3) application in regulating and controlling rice yield.
5. A rice stem base dwarfing control mutant gene is characterized in that the mutant gene is a mutant gene represented by Seq ID No: 1, wherein 11 nucleotides from 5269 to 5279 of the gene sequence are deleted; or the cDNA sequence of said gene is as described in Seq ID No: 2, the 11-base deletion sequence at position 2509-2519 in the cDNA sequence shown in figure 2.
6. A protein encoded by the mutant gene of claim 5, a plasmid, plant expression vector or host cell comprising the mutant gene of claim 4; the mutant gene encodes a protein such as Seq ID No: 4.
7. Use of the mutant gene of claim 5, the protein, plasmid, plant expression vector or host cell of claim 6, wherein said use is:
(1) the application in reducing the height of rice plants;
(2) the application of the rice in improving the lodging resistance of rice;
(3) application in improving rice yield.
8. The use of claim 7, wherein the rice plants in (1), (2) and (3) are rice plants which have SD1 gene but are still too tall.
9. The use of a rice stem base dwarfing control mutant, which contains the mutant gene of claim 5, wherein the mutant is obtained by cross breeding:
(1) the plant height of the rice is reduced;
(2) the lodging resistance of the rice is improved;
(3) the rice yield is improved.
10. The use of the rice stem base dwarf control mutant as claimed in claim 9, wherein the rice in (1), (2) and (3) is rice which has SD1 gene but still has too high plant.
CN202210726272.4A 2022-06-23 2022-06-23 Rice stalk base dwarf control gene, protein, mutant gene, mutant and application thereof Active CN114875050B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210726272.4A CN114875050B (en) 2022-06-23 2022-06-23 Rice stalk base dwarf control gene, protein, mutant gene, mutant and application thereof

Applications Claiming Priority (1)

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CN111848765A (en) * 2020-07-22 2020-10-30 中国水稻研究所 Rice gene OsFBK4 and mutant and application thereof
CN114292868A (en) * 2021-12-29 2022-04-08 武汉大学 Application of rice plant height gene LOC _ Os03g64415 in rice plant type improvement

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CN111848765A (en) * 2020-07-22 2020-10-30 中国水稻研究所 Rice gene OsFBK4 and mutant and application thereof
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