CN114853842B - Method for continuously modifying isolated soy protein based on modified atmosphere auxiliary low-temperature plasma - Google Patents
Method for continuously modifying isolated soy protein based on modified atmosphere auxiliary low-temperature plasma Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
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- A23J3/16—Vegetable proteins from soybean
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention belongs to the technical field of modification of soybean protein isolate, and discloses a method for continuously modifying soybean protein isolate based on modified atmosphere auxiliary low-temperature plasma. The dielectric barrier discharge low-temperature plasma generator is connected with a conveying pipeline of mixed gas of nitrogen, oxygen and carbon dioxide, and the flow rate and the proportion of the mixed gas can be adjusted on line; the soybean protein isolate is continuously sent into a low-temperature plasma generator for treatment through a conveyor belt, and then is sent out along with the conveyor belt and packaged, so that the modified soybean protein isolate is obtained. The invention can continuously process the soy protein isolate, regulate and control the gas components input into the low-temperature plasma processor on line, reduce the modification process of the soy protein isolate, save the modification cost, improve the modification efficiency and facilitate the practical application of the low-temperature plasma modified soy protein isolate technology.
Description
Technical Field
The invention belongs to the technical field of modification of soybean protein isolate, and particularly relates to a method for continuously modifying soybean protein isolate based on modified atmosphere auxiliary low-temperature plasma.
Background
The isolated soy protein is a full-value plant protein with the purity of more than 90 percent, has comprehensive nutrition and low price, and is one of ideal substitutes for animal proteins. The isolated soy protein is a renewable resource, has good biocompatibility, biodegradability and processability, and is widely applied to the fields of foods, medicines, cosmetics and the like. The functional characteristics of the isolated soy protein, such as emulsifying property, solubility, foamability, water holding capacity, gel property and the like, are closely related to the processing characteristics of a plurality of foods, and have great application potential in the food industry. However, commercial isolated soy proteins are typically prepared by an alkaline-soluble acid-precipitating spray-drying process, and the resulting isolated soy proteins have poor solubility and partial functional properties, limiting their use in a variety of industries. Therefore, a great deal of research has been conducted on modification of isolated soy proteins, including physical modification, chemical modification, biological modification. The physical modification is usually heating modification, but if the temperature is too high, the nutritional ingredients of the protein are destroyed, and the edible value of the protein is reduced. The chemical modification method can obviously improve the functional characteristics of the protein, but has serious problems of reagent residue and equipment corrosion and is hardly used at present. The enzymatic modification is the most commonly used method in biological modification, but the enzymatic modification has long time, high cost and strong substrate specificity, and is not suitable for practical application. The exploration of a safe, reliable and efficient protein modification method is a key for expanding the application of the isolated soy protein and is also a hot spot for the research of the isolated soy protein.
The low-temperature plasma refers to charged particles (electrons, ions) and uncharged particles (molecules, excited atoms, metastable atoms, free radicals) generated by excitation of a plurality of excitation sources such as microwaves, light pulses, alternating current or direct current, and ultraviolet rays, gamma rays, beta rays, and the like, and is called a fourth state other than a solid state, a liquid state and a gaseous state under the room temperature condition. Common modes of generation of low temperature plasmas are atmospheric pressure plasma jet (Atmospheric pressure plasma jets, APPJ), dielectric barrier discharge (Dielectric barrier discharge, DBD), corona Discharge (CD), microwave discharge (Microwave discharges, MD). The DBD is the low-temperature plasma generator with the most wide application range, and has the characteristics of wide application range, large working area, multiple carrier gas types and the like. As a non-thermal, safe and environment-friendly physical modification technology, the low-temperature plasma is widely applied to the fields of sewage purification, high polymer material printing, polymer material improvement and the like, and is also a research hot spot for non-thermal processing of foods. Compared with other physical modification methods, the low-temperature plasma is a method for modifying the surface of food by breaking covalent bonds of proteins and initiating various chemical reactions through active oxygen (Reactive Oxygen Species, ROS), active nitrogen (Reactive Nitrogen Species, RNS), free radicals and high-speed electrons generated by the low-temperature plasma, is suitable for thermosensitive food, can clean, sterilize and modify the food under the condition of no exogenous additive, and can better maintain the nutritional value and functional characteristics of the food. Therefore, low temperature plasmas have great potential and prospect in protein modification. However, the conventional low-temperature plasma generally processes the protein solution, so that not only is the protein modification process increased, but also the protein modification cost is increased, and the practical application cannot be performed. In addition, the single low-temperature plasma technology modified protein has the problems of long treatment time, poor modification effect and the like. The plasma modified protein technology is simplified, and the improvement of the protein modification effect is the key of applying the plasma modified protein technology to practice.
Some studies have shown that the difference in oxygen content in the carrier gas of dielectric barrier discharge low temperature plasma (DBD-CP) affects the yield of certain active particles during the process, and that the yield of active particles increases as the oxygen content increases. So that the DBD-CP treatment can be assisted by air conditioning to improve the yield of active substances in the treatment process. The existing gas-conditioned packaged food is researched for low-temperature plasma sterilization and preservation, but a researched object needs to be subjected to gas-conditioned packaging and then low-temperature plasma treatment, so that the gas-conditioned packaged food is complex in treatment process, long in treatment time, high in cost and limited in application. At present, no related research report of using modified atmosphere auxiliary low-temperature plasma for continuous modification of soy protein isolate is found.
Disclosure of Invention
The invention aims to provide a method for continuously modifying soybean protein isolate based on air-conditioning auxiliary low-temperature plasma, which realizes continuous modification of the soybean protein isolate by controlling the air proportion and continuous conveying of a conveying belt on line so as to facilitate practical application of the soybean protein isolate modified by the low-temperature plasma.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for continuously modifying soybean protein isolate based on modified atmosphere assisted low-temperature plasma comprises the following steps:
(1) Connecting a dielectric barrier discharge low-temperature plasma generator with a conveying pipeline of mixed gas of nitrogen, oxygen and carbon dioxide, and adjusting the proportion and the flow rate of the mixed gas;
(2) And (3) conveying the soy isolate protein into a dielectric barrier discharge low-temperature plasma generator through a continuous conveying device for treatment, and obtaining the modified soy isolate protein after treatment.
Preferably, the mixed gas in the step (1) is 60% O 2 +20％N 2 +20％CO 2 。
In the present invention, the flow rate of the mixed gas in the step (1) is preferably 5 to 20L/min, more preferably 10L/min.
Preferably, the continuous conveying device is composed of a conveying belt and a motor, wherein the conveying belt is parallel to the axis of the inner cavity of the dielectric barrier discharge low-temperature plasma generator, one end of the conveying belt is used as an inlet of soybean protein isolate, and the other end of the conveying belt is used as an outlet of modified soybean protein isolate.
Preferably, the speed of the continuous transmission device is 10-50 cm/min, more preferably 30cm/min.
Preferably, the soybean protein isolate is treated in a dielectric barrier discharge low temperature plasma generator in an amount of 30 to 100g/min, more preferably 50g/min.
Preferably, the soybean protein isolate is treated in the dielectric barrier discharge low temperature plasma generator for 60 to 300 seconds, more preferably 240 seconds.
Preferably, the voltage of the dielectric barrier discharge low-temperature plasma generator is 30-110 kV, the frequency is 45-135 Hz, and more preferably, the voltage is 80kV, and the frequency is 120Hz.
In the method, the soybean protein isolate powder is continuously conveyed by the conveyor belt, the proportion of the mixed gas of nitrogen, oxygen and carbon dioxide is adjusted on line, the modification process of the soybean protein isolate can be reduced, the modification cost is saved, the modification effect is improved, the continuous modification of the soybean protein isolate is realized, and the practical application of the low-temperature plasma modified soybean protein isolate technology is facilitated.
Compared with the traditional modification method, the continuous modification of the soybean protein isolate by the modified atmosphere packaging auxiliary dielectric barrier discharge low-temperature plasma technology has the following advantages:
1. compared with the traditional protein modification means, the dielectric barrier discharge low-temperature plasma continuous modification soybean protein isolate technology is a non-thermal physical modification method, can modify the protein under the condition of no any exogenous additive, can protect heat-sensitive substances in the soybean protein isolate, and has the advantages of safety, greenness, no pollution and the like;
2. compared with the traditional low-temperature plasma modified protein, the modified soybean protein can be continuously conveyed into an isothermal plasma generator for treatment by the modified low-temperature plasma with the aid of the modified dielectric barrier discharge, and the gas proportion of nitrogen, oxygen and carbon dioxide can be adjusted on line, so that continuous modification of the soybean protein isolate is realized. In addition, the continuous transmission of the soy protein isolate and the on-line regulation of the gas proportion can reduce the protein modification process, save the protein modification cost and facilitate the practical application.
3. The soybean protein isolate after continuous modification of the modified dielectric barrier discharge low-temperature plasma is effectively improved in solubility, gel property, emulsifying stability, foamability and foam stability, and can be widely applied to the fields of foods, medicines, cosmetics and the like.
Description of the drawings:
FIG. 1 is a process flow diagram of continuous modification of isolated soy protein based on modified atmosphere assisted low temperature plasma in accordance with the present invention.
FIG. 2 is a schematic diagram of a dielectric barrier discharge low temperature plasma apparatus for treating isolated soy protein in accordance with an embodiment of the present invention.
Icon: the device comprises a soybean protein isolate inlet 1, a gas controller 2, a gas inlet 3, a conveyor belt 4, a valve 5, a flowmeter 6, an oxygen cylinder 7, a nitrogen cylinder 8, a carbon dioxide cylinder 9, a high-voltage power supply 10, a high-voltage positive electrode 11, a high-voltage dielectric barrier discharge generator 12, a high-voltage negative electrode 13, a tail gas outlet 14, a valve 15, a grounding system 16, a flowmeter 17, a tail gas analyzer 18, a tail gas treatment 19 and a soybean protein isolate outlet 20.
Detailed Description
Embodiments of the invention in specific applications are described in detail below and are only a few of the embodiments of the invention. Based on the embodiments of the present invention, other embodiments that may be obtained by those of ordinary skill in the art without making any inventive effort are within the scope of the present invention.
In the following examples, the dielectric barrier discharge low-temperature plasma generator is adopted for the air-conditioning auxiliary low-temperature plasma treatment, and the structural schematic diagram of the equipment is shown in fig. 1. The device comprises a dielectric barrier discharge low-temperature plasma generator 11, wherein a high-voltage positive electrode 10 and a high-voltage negative electrode 12 are arranged on the upper side and the lower side of the dielectric barrier discharge low-temperature plasma generator 11, and the positive electrode and the negative electrode are connected with a high-voltage power supply 10. The oxygen bottle 7, the nitrogen bottle 8 and the carbon dioxide bottle 9 are connected to the gas inlet 3 of the dielectric barrier discharge low-temperature plasma generator 11 through gas pipelines, the connected gas pipelines are provided with a flowmeter 6 and a valve 5, and the flowmeter 6 is connected with the gas controller 2 and is used for monitoring and adjusting the proportion of mixed gas; the inside of the dielectric barrier discharge low-temperature plasma generator 11 is provided with a conveyor belt 4, one end of the conveyor belt 4 is used as a soybean protein isolate inlet 1, the other end of the conveyor belt is used as a soybean protein isolate outlet 20, the soybean protein isolate is conveyed to the dielectric barrier discharge low-temperature plasma generator 11 from one end to be treated by controlling the conveyor belt, the soybean protein isolate is modified under the assistance of mixed gas, the modified soybean protein isolate is output from the other end, and the mixed gas proportion on-line control and the continuous modification of the soybean protein isolate can be realized; the soybean protein isolate outlet of the low-temperature plasma generator 12 is also provided with a tail gas outlet 14, the tail gas outlet 14 is connected with a tail gas analyzer 18 and a tail gas treatment device 19 through a tail gas discharge pipeline, and is used for analyzing, treating and discharging treated gas, and the tail gas flow is controlled through a valve 15 and a flowmeter 17 on the tail gas discharge pipeline.
Example 1
The method for continuously modifying the soybean protein isolate based on the modified atmosphere auxiliary low-temperature plasma in the embodiment is specifically as follows:
feeding soybean protein isolate powder into a dielectric barrier discharge low temperature plasma generator at a loading of 100g/min via a 20cm/min conveyor belt, and charging mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 30% O) at a flow rate of 8L/min 2 +50％N 2 +20％CO 2 ) The method comprises the steps of carrying out a first treatment on the surface of the The mixed gas and the soy protein isolate in the generator are treated by the low-temperature plasma, the treatment time is 120s, the treatment voltage is 40kV, and the treatment frequency is 75Hz; after the treatment is finished, the gas in the plasma generator is discharged for analysis treatment, and the modified soy protein isolate powder is sent out and packaged to obtain the modified commercial soy protein isolate. As compared with conventional low-temperature plasma treatment, the solubility, gel property, emulsifying property and emulsion stability, foamability and foam stability of the isolated soybean protein powder of this example are respectively improved by 9.6%, 7.7%, 28.2%, 69.3%, 13.2%, 8.1％。
Example 2
Feeding soybean protein isolate powder into a dielectric barrier discharge low-temperature plasma generator at a loading of 80g/min via a 40cm/min conveyor belt, and charging mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 40% O) at a flow rate of 20L/min 2 +40％N 2 +20％CO 2 ) The method comprises the steps of carrying out a first treatment on the surface of the The mixed gas and the soy protein isolate in the generator are treated by the low-temperature plasma, the treatment time is 180s, the treatment voltage is 70kV, and the treatment frequency is 90Hz; after the treatment is finished, the gas in the plasma generator is discharged for analysis treatment, and the modified soy protein isolate powder is sent out and packaged to obtain the modified commercial soy protein isolate. As compared with the traditional low-temperature plasma treatment, the solubility, gel property, emulsifying property and emulsion stability, foamability and foam stability of the soybean protein isolate powder are improved by 12.6%, 7.7%, 51.8%, 93.0%, 17.5% and 9.2% respectively.
Example 3
Feeding soybean protein isolate powder into a dielectric barrier discharge low temperature plasma generator at a loading of 30g/min via a 50cm/min conveyor belt, and charging mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 50% O) at a flow rate of 15L/min 2 +30％N 2 +20％CO 2 ) The method comprises the steps of carrying out a first treatment on the surface of the The mixed gas and the soy protein isolate in the generator are treated by the low-temperature plasma, the treatment time is 300s, the treatment voltage is 110kV, and the treatment frequency is 135Hz; after the treatment is finished, the gas in the plasma generator is discharged for analysis treatment, and the modified soy protein isolate powder is sent out and packaged to obtain the modified commercial soy protein isolate. As compared with the traditional low-temperature plasma treatment, the solubility, gel property, emulsifying property and emulsion stability, foamability and foam stability of the soybean protein isolate powder are respectively improved by 19.0%, 16.3%, 46.4%, 98.8%, 19.6% and 9.3%.
Example 4
Mixing soybean protein isolate powder with 50g ∈ -The load of min is sent into a dielectric barrier discharge low-temperature plasma generator through a conveyor belt of 30cm/min, and then mixed gas of nitrogen, oxygen and carbon dioxide (the volume ratio is 60 percent O) with a flow rate of 10L/min is filled into the generator 2 +20％N 2 +20％CO 2 ) The method comprises the steps of carrying out a first treatment on the surface of the The mixed gas and the soy protein isolate in the generator are treated by the low-temperature plasma, the treatment time is 240s, the treatment voltage is 80kV, and the treatment frequency is 120Hz; after the treatment is finished, the gas in the plasma generator is discharged for analysis treatment, and the modified soy protein isolate powder is sent out and packaged to obtain the modified commercial soy protein isolate. As compared with the traditional low-temperature plasma treatment, the solubility, gel property, emulsifying property and emulsion stability, foamability and foam stability of the soybean protein isolate powder of the embodiment are respectively improved by 27.9%, 24.0%, 51.2%, 108.6%, 23.8% and 10.0%.
Claims (8)
1. The method for continuously modifying the soybean protein isolate based on the modified atmosphere auxiliary low-temperature plasma is characterized by comprising the following steps of:
(1) Connecting a dielectric barrier discharge low-temperature plasma generator with a conveying pipeline of mixed gas of nitrogen, oxygen and carbon dioxide, and adjusting the proportion and the flow rate of the mixed gas; the mixed gas is 60% O 2 +20%N 2 +20%CO 2 The voltage of the dielectric barrier discharge low-temperature plasma generator is 30-110 kV, and the frequency is 45-135 Hz;
(2) And (3) conveying the soy isolate protein into a dielectric barrier discharge low-temperature plasma generator through a continuous conveying device for treatment, and obtaining the modified soy isolate protein after treatment.
2. The method for continuously modifying soybean protein isolate based on modified atmosphere assisted low-temperature plasma according to claim 1, wherein the flow rate of the mixed gas in the step (1) is 5-20L/min.
3. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma according to claim 1, wherein the flow rate of the mixed gas in step (1) is 10L/min.
4. The method for continuously modifying soybean protein isolate based on air-conditioning auxiliary low-temperature plasma according to claim 1, wherein the continuous conveying device consists of a conveying belt and a motor, the conveying belt is parallel to the axis of the inner cavity of the dielectric barrier discharge low-temperature plasma generator, one end of the conveying belt is used as an inlet of the soybean protein isolate, and the other end of the conveying belt is used as an outlet of the modified soybean protein isolate.
5. The method for continuously modifying soybean protein isolate based on modified atmosphere assisted low temperature plasma according to claim 1 or 4, wherein the speed of the continuous conveying device is 10-50 cm/min.
6. The method for continuously modifying soybean protein isolate based on modified atmosphere assisted low-temperature plasma according to claim 1, wherein the amount of soybean protein isolate processed in a dielectric barrier discharge low-temperature plasma generator is 30-100 g/min.
7. The method for continuously modifying soybean protein isolate based on modified atmosphere assisted low-temperature plasma according to claim 1, wherein the time for processing the soybean protein isolate in a dielectric barrier discharge low-temperature plasma generator is 60-300 s.
8. The method for continuously modifying soy protein isolate based on modified atmosphere assisted low temperature plasma according to claim 1, wherein the dielectric barrier discharge low temperature plasma generator has a voltage of 80kV and a frequency of 120Hz.
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