CN114853842A - Method for continuously modifying isolated soy protein based on gas-conditioning-assisted low-temperature plasma - Google Patents
Method for continuously modifying isolated soy protein based on gas-conditioning-assisted low-temperature plasma Download PDFInfo
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Abstract
The invention belongs to the technical field of modification of isolated soy protein, and discloses a method for continuously modifying isolated soy protein based on gas-conditioning auxiliary low-temperature plasma. The dielectric barrier discharge low-temperature plasma generator is connected with a conveying pipeline of the mixed gas of nitrogen, oxygen and carbon dioxide, and the flow rate and the proportion of the mixed gas can be adjusted on line; the soybean protein isolate is continuously sent into a low-temperature plasma generator for processing through a conveyor belt, and then is sent out along with the conveyor belt and packaged to obtain the modified soybean protein isolate. The invention can continuously process the soybean protein isolate, regulate and control the gas components input into the low-temperature plasma processor on line, reduce the modification process of the soybean protein isolate, save the modification cost, improve the modification efficiency and facilitate the practical application of the low-temperature plasma modified soybean protein isolate technology.
Description
Technical Field
The invention belongs to the technical field of modification of isolated soy protein, and particularly relates to a method for continuously modifying isolated soy protein based on gas-regulated auxiliary low-temperature plasma.
Background
The soybean protein isolate is a complete vegetable protein with the purity of more than 90 percent, has comprehensive nutrition and low price, and is one of ideal substitutes of animal protein. The soybean protein isolate is a renewable resource, has good biocompatibility, biodegradability and processability, and is widely applied to the fields of food, medicines, cosmetics and the like. The functional characteristics of the soybean protein isolate, such as emulsibility, solubility, foamability, water-holding capacity and gel property, are closely related to the processing characteristics of a plurality of foods, and have great application potential in the food industry. However, the commercial soybean protein isolate is usually prepared by an alkali-soluble acid-deposited spray-drying process, and the solubility and partial functional properties of the obtained soybean protein isolate are poor, so that the application of the soybean protein isolate in various industries is limited. Therefore, many studies have been made on the modification of isolated soy protein, including physical modification, chemical modification, and biological modification. The physical modification is usually heating modification, but if the temperature is too high, the nutritional ingredients of the protein can be damaged, and the edible value of the protein is reduced. The chemical modification method can obviously improve the functional characteristics of the protein, but the problems of reagent residue and equipment corrosion are serious, and the chemical modification method is hardly used at present. Enzymatic modification is the most common method in biological modification, but enzymatic modification is long in time, high in cost, strong in substrate specificity and not suitable for practical application. The exploration of a safe, reliable and efficient protein modification method is the key for widening the application of the soybean protein isolate and is a hotspot for the research of the soybean protein isolate.
The low-temperature plasma refers to charged particles (electrons and ions) and uncharged particles (molecules, excited atoms, metastable atoms and radicals) generated by excitation of neutral gas by various excitation sources such as microwaves, light pulses, alternating current or direct current at room temperature, ultraviolet rays, gamma rays, beta rays and the like, and is called as a fourth state other than a solid state, a liquid state and a gaseous state. Common methods for generating low-temperature plasma include Atmospheric Pressure Plasma Jet (APPJ), Dielectric Barrier Discharge (DBD), Corona Discharge (CD), and Microwave Discharge (MD). Among them, the DBD is a low-temperature plasma generator most widely used, and has the characteristics of wide application range, large working area, various types of carrier gas, and the like. As a non-thermal, safe and environment-friendly physical modification technology, low-temperature plasma has been widely applied to the fields of sewage purification, high polymer material printing, polymer material improvement and the like, and is also a research hotspot for non-thermal processing of current food. Compared with other physical modification methods, the low-temperature plasma is a method for modifying the surface of food by breaking covalent bonds in protein and initiating various chemical reactions through Reactive Oxygen Species (ROS), Reactive Nitrogen Species (RNS), free radicals and high-speed electrons generated by the low-temperature plasma, is suitable for thermosensitive food, can clean, sterilize and modify the food without external additives, and can better maintain the nutritional value and functional characteristics of the food. Therefore, the low-temperature plasma has great potential and prospect in the aspect of protein modification. However, the conventional low-temperature plasma generally treats protein solution, not only increases protein modification process but also increases protein modification cost, and cannot be practically applied. In addition, the single low-temperature plasma technology modified protein also has the problems of long treatment time, poor modification effect and the like. The key to the practical application of the plasma modified protein technology is to simplify the plasma modified protein process and improve the protein modification effect.
Some studies have shown that the oxygen content in the carrier gas of the dielectric barrier discharge low temperature plasma (DBD-CP) affects the yield of some active species during processing, and that as the oxygen content increases, the yield of active species increases. Therefore, the DBD-CP treatment can be assisted by air regulation so as to improve the yield of active substances in the treatment process. The existing research on low-temperature plasma sterilization and preservation of food packaged in controlled atmosphere is carried out, but a research object needs to carry out controlled atmosphere packaging firstly and then carry out low-temperature plasma treatment, so that the processing technology is complex, the processing time is long, the cost is high, and certain application limitations exist. At present, no relevant research report of the modified atmosphere assisted low-temperature plasma for continuous modification of the isolated soy protein is found.
Disclosure of Invention
The invention aims to provide a method for continuously modifying soybean protein isolate based on gas-conditioning-assisted low-temperature plasma, which realizes continuous modification of the soybean protein isolate by controlling gas proportion on line and conveying the soybean protein isolate continuously by a conveyor belt so as to facilitate the practical application of modifying the soybean protein isolate by the low-temperature plasma.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for continuously modifying isolated soy protein based on modified atmosphere assisted low-temperature plasma comprises the following steps:
(1) connecting a dielectric barrier discharge low-temperature plasma generator with a conveying pipeline of mixed gas of nitrogen, oxygen and carbon dioxide, and adjusting the proportion and the flow rate of the mixed gas;
(2) and (3) conveying the soybean protein isolate into a dielectric barrier discharge low-temperature plasma generator through a continuous transmission conveying device for treatment, and obtaining the modified soybean protein isolate after treatment.
Preferably, the mixed gas in the step (1) is 60% O 2 +20％N 2 +20％CO 2 。
In the present invention, the flow rate of the mixed gas in the step (1) is preferably 5 to 20L/min, and more preferably 10L/min.
Preferably, the continuous transmission conveying device is composed of a conveying belt and a motor, the conveying belt is parallel to the axis of an inner cavity of the dielectric barrier discharge low-temperature plasma generator, one end of the conveying belt is used as an inlet of the soybean protein isolate, and the other end of the conveying belt is used as an outlet of the modified soybean protein isolate.
Preferably, the speed of the continuous transmission conveying device is 10-50 cm/min, and more preferably 30 cm/min.
In the invention, the treatment amount of the soybean protein isolate in the dielectric barrier discharge low-temperature plasma generator is preferably 30-100 g/min, and more preferably 50 g/min.
In the invention, the treatment time of the soybean protein isolate in the dielectric barrier discharge low-temperature plasma generator is preferably 60-300 s, and more preferably 240 s.
Preferably, the voltage of the dielectric barrier discharge low-temperature plasma generator is 30-110 kV, the frequency is 45-135 Hz, and more preferably, the voltage is 80kV, and the frequency is 120 Hz.
In the method, the soybean protein isolate powder is continuously conveyed by the conveyor belt and the proportion of the mixed gas of nitrogen, oxygen and carbon dioxide is adjusted on line, so that the modification process of the soybean protein isolate can be reduced, the modification cost is saved, the modification effect is improved, the continuous modification of the soybean protein isolate is realized, and the practical application of the low-temperature plasma modified soybean protein isolate technology is facilitated.
Compared with the traditional modification method, the method for continuously modifying the isolated soy protein by the modified atmosphere packaging auxiliary dielectric barrier discharge low-temperature plasma technology has the following advantages:
1. compared with the traditional protein modification means, the technology for continuously modifying the isolated soy protein by the dielectric barrier discharge low-temperature plasma is a non-thermal physical modification method, can modify the protein without any external additive, can protect the heat-sensitive substances in the isolated soy protein, and has the advantages of safety, greenness, no pollution and the like;
2. compared with the traditional low-temperature plasma modified protein, the modified atmosphere auxiliary dielectric barrier discharge low-temperature plasma can continuously convey the soybean protein isolate into the isothermal plasma generator for treatment through the conveyor belt, and the gas proportion of nitrogen, oxygen and carbon dioxide can be adjusted on line, so that the continuous modification of the soybean protein isolate is realized. In addition, the continuous conveying of the isolated soy protein and the on-line adjustment of the gas ratio can reduce the protein modification process, save the protein modification cost and facilitate the practical application.
3. The soybean protein isolate continuously modified by the gas-conditioning auxiliary dielectric barrier discharge low-temperature plasma effectively improves the solubility, the gel property, the emulsifying stability, the foaming property and the foam stability of the soybean protein isolate, and can widen the application of the soybean protein isolate in the fields of food, medicine, cosmetics and the like.
Description of the drawings:
FIG. 1 is a process flow chart of continuous modification of isolated soy protein based on modified atmosphere assisted low temperature plasma.
FIG. 2 is a schematic structural diagram of a dielectric barrier discharge low-temperature plasma device for treating soy protein isolate according to an embodiment of the present invention.
Icon: the device comprises a soybean protein isolate inlet 1, a gas controller 2, a gas inlet 3, a conveyor belt 4, a valve 5, a flowmeter 6, an oxygen cylinder 7, a nitrogen cylinder 8, a carbon dioxide cylinder 9, a high-voltage power supply 10, a high-voltage anode 11, a high-voltage dielectric barrier discharge generator 12, a high-voltage cathode 13, a tail gas outlet 14, a valve 15, a grounding system 16, a flowmeter 17, a tail gas analyzer 18, a tail gas treatment 19 and a soybean protein isolate outlet 20.
Detailed Description
The following detailed description of embodiments of the invention in specific applications is provided as an illustration of some embodiments of the invention. Other embodiments, which can be derived by one of ordinary skill in the art from the embodiments of the present invention without making any creative effort, are within the protection scope of the present invention.
The gas-adjusting auxiliary low-temperature plasma treatment in the following example adopts a dielectric barrier discharge low-temperature plasma generator, and the structural schematic diagram of the equipment is shown in figure 1. The device comprises a dielectric barrier discharge low-temperature plasma generator 11, wherein a high-voltage positive electrode 10 and a high-voltage negative electrode 12 are arranged on the upper side and the lower side of the dielectric barrier discharge low-temperature plasma generator 11, and the positive electrode and the negative electrode are connected with a high-voltage power supply 10. The oxygen cylinder 7, the nitrogen cylinder 8 and the carbon dioxide cylinder 9 are connected with a gas inlet 3 of a dielectric barrier discharge low-temperature plasma generator 11 through gas pipelines, a flow meter 6 and a valve 5 are arranged on the connected gas pipelines, and the flow meter 6 is connected with a gas controller 2 and used for monitoring and adjusting the proportion of mixed gas; the dielectric barrier discharge low-temperature plasma generator 11 is internally provided with a conveyor belt 4, one end of the conveyor belt 4 is used as a soybean protein isolate inlet 1, the other end of the conveyor belt is used as a soybean protein isolate outlet 20, the soybean protein isolate is conveyed from one end to the dielectric barrier discharge low-temperature plasma generator 11 for treatment by controlling the conveyor belt, the soybean protein isolate is modified under the assistance of mixed gas, and the modified soybean protein isolate is output from the other end, so that the online control of the proportion of the mixed gas and the continuous modification of the soybean protein isolate can be realized; the soybean protein isolate outlet of the low-temperature plasma generator 12 is also provided with a tail gas outlet 14, the tail gas outlet 14 is connected with a tail gas analyzer 18 and a tail gas treatment device 19 through a tail gas discharge pipeline, and is used for analyzing, treating and discharging the treated gas, and the tail gas flow is controlled through a valve 15 and a flowmeter 17 on the tail gas discharge pipeline.
Example 1
The method for continuously modifying the isolated soy protein based on the modified atmosphere assisted low-temperature plasma in the embodiment is specifically as follows:
feeding soybean protein isolate powder into a dielectric barrier discharge low-temperature plasma generator through a conveyor belt of 20cm/min at a loading capacity of 100g/min, and then filling a certain proportion of mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 30% O) into the generator at a flow rate of 8L/min 2 +50％N 2 +20％CO 2 ) (ii) a The low-temperature plasma is used for processing the mixed gas and the soybean protein isolate in the generator, the processing time is 120s, the processing voltage is 40kV, and the processing frequency is 75 Hz; and after the treatment is finished, discharging the gas in the plasma generator for analysis treatment, and delivering and packaging the modified soybean protein isolate powder to obtain the modified commercial soybean protein isolate. Through determination, the soybean protein isolate powder of the embodiment is treated with the traditional low-temperature plasmaCompared with the prior art, the solubility, the gel property, the emulsifying stability, the foaming property and the foam stability are respectively improved by 9.6 percent, 7.7 percent, 28.2 percent, 69.3 percent, 13.2 percent and 8.1 percent.
Example 2
Feeding soybean protein isolate powder into a dielectric barrier discharge low-temperature plasma generator through a conveyor belt of 40cm/min at a loading capacity of 80g/min, and then filling a certain proportion of mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 40% O) into the generator at a flow rate of 20L/min 2 +40％N 2 +20％CO 2 ) (ii) a The low-temperature plasma is used for treating the mixed gas and the soybean protein isolate in the generator, the treatment time is 180s, the treatment voltage is 70kV, and the treatment frequency is 90 Hz; after the treatment, the gas in the plasma generator was discharged for analysis treatment, and the modified soybean protein isolate powder was sent out and packaged to obtain a modified commercial soybean protein isolate. Through determination, compared with the traditional low-temperature plasma treatment, the solubility, the gel property, the emulsifying stability, the foaming property and the foam stability of the soybean protein isolate powder are respectively improved by 12.6%, 7.7%, 51.8%, 93.0%, 17.5% and 9.2%.
Example 3
Feeding soybean protein isolate powder into a dielectric barrier discharge low-temperature plasma generator through a 50cm/min conveying belt at a loading capacity of 30g/min, and then filling a certain proportion of nitrogen, oxygen and carbon dioxide mixed gas (volume ratio is 50% O) into the generator at a flow rate of 15L/min 2 +30％N 2 +20％CO 2 ) (ii) a The low-temperature plasma is used for processing the mixed gas and the soybean protein isolate in the generator, the processing time is 300s, the processing voltage is 110kV, and the processing frequency is 135 Hz; and after the treatment is finished, discharging the gas in the plasma generator for analysis treatment, and delivering and packaging the modified soybean protein isolate powder to obtain the modified commercial soybean protein isolate. Through determination, compared with the traditional low-temperature plasma treatment, the solubility, the gel property, the emulsibility, the emulsion stability, the foamability and the foam stability of the soybean protein isolate powder are respectively improved by 19.0 percent and 16.3 percent、46.4％、98.8％、19.6％、9.3％。
Example 4
Feeding the soybean protein isolate powder into a dielectric barrier discharge low-temperature plasma generator through a conveyor belt of 30cm/min at a loading capacity of 50g/min, and then filling a certain proportion of mixed gas of nitrogen, oxygen and carbon dioxide (volume ratio of 60% O) into the generator at a flow rate of 10L/min 2 +20％N 2 +20％CO 2 ) (ii) a The low-temperature plasma is used for processing the mixed gas and the soybean protein isolate in the generator, the processing time is 240s, the processing voltage is 80kV, and the processing frequency is 120 Hz; and after the treatment is finished, discharging the gas in the plasma generator for analysis treatment, and delivering and packaging the modified soybean protein isolate powder to obtain the modified commercial soybean protein isolate. Through determination, compared with the traditional low-temperature plasma treatment, the solubility, the gel property, the emulsifying stability, the foaming property and the foam stability of the soybean protein isolate powder are respectively improved by 27.9%, 24.0%, 51.2%, 108.6%, 23.8% and 10.0%.
Claims (10)
1. A method for continuously modifying isolated soy protein based on modified atmosphere assisted low-temperature plasma is characterized by comprising the following steps:
(1) connecting a dielectric barrier discharge low-temperature plasma generator with a conveying pipeline of mixed gas of nitrogen, oxygen and carbon dioxide, and adjusting the proportion and the flow rate of the mixed gas;
(2) and (3) conveying the soybean protein isolate into a dielectric barrier discharge low-temperature plasma generator through a continuous transmission device for treatment, and obtaining the modified soybean protein isolate after the treatment.
2. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1, wherein the mixed gas in step (1) is 60% O 2 +20％N 2 +20％CO 2 。
3. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1, wherein the flow rate of the mixed gas in step (1) is 5-20L/min.
4. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1, wherein the flow rate of the mixed gas in step (1) is 10L/min.
5. The method for continuously modifying soybean protein isolate based on modified atmosphere assisted low-temperature plasma as claimed in claim 1, wherein the continuous conveying device is composed of a conveyor belt and a motor, the conveyor belt is parallel to the axis of the inner cavity of the dielectric barrier discharge low-temperature plasma generator, one end of the conveyor belt is used as the inlet of the soybean protein isolate, and the other end of the conveyor belt is used as the outlet of the modified soybean protein isolate.
6. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1 or 5, wherein the continuous transport conveyor has a speed of 10-50 cm/min.
7. The method for continuously modifying the isolated soy protein based on the modified atmosphere assisted low-temperature plasma as claimed in claim 1, wherein the processing amount of the isolated soy protein in the dielectric barrier discharge low-temperature plasma generator is 30-100 g/min.
8. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1, wherein the isolated soy protein is treated in the dielectric barrier discharge low temperature plasma generator for 60-300 s.
9. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 1, wherein the voltage of the dielectric barrier discharge low temperature plasma generator is 30-110 kV, and the frequency is 45-135 Hz.
10. The method for continuously modifying isolated soy protein based on modified atmosphere assisted low temperature plasma as claimed in claim 9, wherein the voltage of the dielectric barrier discharge low temperature plasma generator is 80kV, and the frequency is 120 Hz.
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| CN116349905A (en) * | 2023-04-20 | 2023-06-30 | 江南大学 | Device and method for removing dangers on medlar under normal pressure |
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| CN116349905A (en) * | 2023-04-20 | 2023-06-30 | 江南大学 | Device and method for removing dangers on medlar under normal pressure |
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