CN114836186A - Biological thickened oil viscosity reducer and application thereof - Google Patents
Biological thickened oil viscosity reducer and application thereof Download PDFInfo
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- 239000003638 chemical reducing agent Substances 0.000 title claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 109
- 239000003921 oil Substances 0.000 claims abstract description 99
- 230000000813 microbial effect Effects 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000009467 reduction Effects 0.000 claims abstract description 17
- 239000003876 biosurfactant Substances 0.000 claims abstract description 10
- 239000004094 surface-active agent Substances 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 8
- 239000012190 activator Substances 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims description 68
- 230000004151 fermentation Effects 0.000 claims description 68
- 230000001580 bacterial effect Effects 0.000 claims description 49
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 28
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 28
- 238000003756 stirring Methods 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 24
- 238000012258 culturing Methods 0.000 claims description 23
- 238000011081 inoculation Methods 0.000 claims description 21
- 238000011068 loading method Methods 0.000 claims description 19
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 16
- 238000009630 liquid culture Methods 0.000 claims description 16
- -1 phenyl stearamidopropyl dimethyl aminoacetate Chemical compound 0.000 claims description 15
- 241000589291 Acinetobacter Species 0.000 claims description 14
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 14
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 14
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 14
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 14
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 14
- 235000002867 manganese chloride Nutrition 0.000 claims description 14
- 239000011565 manganese chloride Substances 0.000 claims description 14
- 229940099607 manganese chloride Drugs 0.000 claims description 14
- 235000013379 molasses Nutrition 0.000 claims description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 14
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- 235000010344 sodium nitrate Nutrition 0.000 claims description 14
- 239000004317 sodium nitrate Substances 0.000 claims description 14
- 241000187561 Rhodococcus erythropolis Species 0.000 claims description 13
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 12
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 12
- 239000011550 stock solution Substances 0.000 claims description 11
- 241000186146 Brevibacterium Species 0.000 claims description 10
- 241000193401 Clostridium acetobutylicum Species 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- 230000003213 activating effect Effects 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 241000588697 Enterobacter cloacae Species 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 6
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims description 5
- 108010028921 Lipopeptides Proteins 0.000 claims description 5
- 241000588843 Ochrobactrum Species 0.000 claims description 5
- 239000005696 Diammonium phosphate Substances 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 4
- 229930195729 fatty acid Natural products 0.000 claims description 4
- 239000000194 fatty acid Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 239000008398 formation water Substances 0.000 claims description 2
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- 229930182470 glycoside Natural products 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 241000894007 species Species 0.000 claims 1
- 239000010779 crude oil Substances 0.000 abstract description 30
- 238000011084 recovery Methods 0.000 abstract description 7
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 125000003368 amide group Chemical group 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- 230000006872 improvement Effects 0.000 description 8
- 238000002156 mixing Methods 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000003208 petroleum Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 125000004122 cyclic group Chemical group 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 150000002596 lactones Chemical class 0.000 description 3
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- 239000011435 rock Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 229920000704 biodegradable plastic Polymers 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000588814 Ochrobactrum anthropi Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000010426 asphalt Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
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- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
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- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of viscosity reduction of thick oil in oil fields, in particular to a biological thick oil viscosity reducer and application thereof, wherein the biological thick oil viscosity reducer is prepared from the following components in parts by mass: 1-20 parts of functional microbial liquid, 1-10 parts of biosurfactant, 0.025-5 parts of bio-based surfactant and 65-97.775 parts of bio-activator solution. The biological thick oil viscosity reducer is mainly applied to thick oil viscosity reduction in a stratum, wherein the oil reservoir temperature ranges from 20 ℃ to 80 ℃, and the thick oil viscosity ranges from 1000 mPa · s to 50000 mPa · s. The components of the biological thickened oil viscosity reducer are biodegradable products, and the biological thickened oil viscosity reducer has the characteristics of being green, non-toxic, pollution-free, harmless to stratum and the like; the viscosity of the thickened oil in the stratum is reduced, and the effect of lifting or pipe transportation viscosity reduction can be achieved after treatment; the viscosity reduction rate can reach 95 to 99 percent aiming at different thickened oils; the recovery rate of crude oil can be improved to a higher degree, and the recovery rate of crude oil can be improved by more than 14% after the biological thick oil viscosity reducer is subjected to water flooding.
Description
Technical Field
The invention relates to the technical field of viscosity reduction of thick oil in an oil field, in particular to a biological thick oil viscosity reducer and application thereof.
Background
In the global petroleum resources, a large part of the petroleum resources belong to unconventional petroleum resources and have the characteristics of high condensation and high viscosity and the like. In the process of pipeline transportation of crude oil, when the temperature of the inner wall of a pipeline is lower than the cloud point of the crude oil, the asphaltene and the wax in the crude oil can be precipitated and separated out and attached to the wall of a cold pipeline, so that the oil flow area of the pipeline is reduced, the flow resistance is increased, and even a transportation line is blocked in severe cases, so that production stop is caused, the petroleum production efficiency is greatly reduced, and huge economic loss is caused. At present, the yield of heavy crude oil (thick oil) is higher and higher, and the viscosity of the heavy crude oil is too high in the transportation process, so that the safety production is influenced, and the production and operation cost is too high. Therefore, the asphalt and wax can be effectively removed, and the viscosity is reduced, so that the method has important significance for the exploitation and transportation of crude oil. At present, in the process of crude oil extraction and transportation, the common viscosity reduction method is a heating method, a chemical reagent adding technology and the like. Although the methods have quick effect, the methods cannot change long-chain hydrocarbon components such as wax, asphaltene and colloid which cause excessive viscosity in the crude oil, and can cause secondary pollution problems, and have poor economical efficiency and harsh use conditions.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a biological thick oil viscosity reducer.
The invention relates to a biological thick oil viscosity reducer, which is prepared from the following components in parts by mass: 1-20 parts of functional microbial liquid, 1-10 parts of biosurfactant, 0.025-5 parts of bio-based surfactant and 65-97.775 parts of bio-activator solution.
As a further improvement of the invention, the functional microbial liquid is prepared by fermenting and culturing target functional microbial strains and a liquid culture medium;
the preparation method of the functional microbial liquid comprises the following steps:
a. activating a target functional strain: respectively picking out 1 ring of target strains on the inclined plane to 100mL of liquid culture medium for activation culture, and performing shake culture at 120 rpm for 16-48 h at the temperature of a target oil reservoir to obtain an activated bacterial liquid;
b. preparing a seed solution: transferring the activated bacterial liquid prepared in the step a into a 5L shake flask for culture, wherein the liquid loading capacity of the 5L shake flask is 1.5L, and the seed bacterial liquid is prepared by shake culture at 120 rpm for 16-48 h at the target oil reservoir temperature;
c. fermentation culture of first-stage seeds: b, fermenting the seed bacterial liquid prepared in the step b, introducing the fermented seed bacterial liquid into a 100L primary seed tank, wherein the liquid loading amount of the seed tank is 70%, the inoculation amount is 2%, the stirring speed is 100 r/min, the pH value is controlled to be 6.0-8.0, and the primary seed fermentation liquid is prepared after culturing for 16-48 h at the oil reservoir temperature;
d. and (3) secondary seed fermentation culture: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank of 1t, wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 rpm, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the oil reservoir temperature;
e. culturing in a fermentation tank: d, introducing the secondary seed solution prepared in the step d into a fermentation tank of 10t, controlling the pH value of the process to be 7.0-8.0 according to 70% liquid loading amount and 10% inoculation amount in the fermentation tank, controlling the stirring speed to be 50 rpm at the oil reservoir temperature, and culturing for 24-72 h to prepare a target functional strain fermentation stock solution;
the liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: 0.1-5 parts of molasses, 0.1-5 parts of white oil, 0.01-0.3 part of yeast powder, 0.1-2 parts of monopotassium phosphate, 0.1-2 parts of diammonium phosphate, 0.001-0.05 part of manganese chloride, 0.01-0.1 part of magnesium sulfate, 0.1-3 parts of sodium nitrate, 0.1-2 parts of rhamnolipid and 80.55-99.38 parts of water.
As a further improvement of the invention, the target functional microorganism strains comprise one or more of Acinetobacter, Brevibacterium, Bacillus, Enterobacter cloacae, Ochrobactrum, Clostridium acetobutylicum, Pseudomonas aeruginosa and Rhodococcus erythropolis.
As a further improvement of the invention, the target functional microbial strain can be a single strain or a plurality of strains. If the bacterial strain is multiple, the ratio of each bacterial liquid to each bacterial liquid is 0-3: … …, 0-3.
As a further improvement of the invention, the biosurfactant is one or more of lipopeptide, rhamnolipid and sophorolipid which are combined in any proportion.
As a further improvement of the invention, the bio-based surfactant is one or more of phenyl octadecanamido propyl dimethylamino acetate, alkyl glycoside, sucrose fatty acid ester and glycerol mono fatty acid ester which are combined in any proportion.
As a further improvement of the invention, the biological activator solution comprises, by mass, 0.1-5 parts of molasses, 0.01-5 parts of white oil, 0.01-0.3 part of yeast powder, 0.1-2 parts of monopotassium phosphate, 0.1-2 parts of diammonium hydrogen phosphate, 0.001-0.05 part of manganese chloride, 0.01-0.1 part of magnesium sulfate, 0.1-3 parts of sodium nitrate, 0.1-2 parts of rhamnolipid and 80.55-99.47 parts of water.
As a further improvement of the invention, the water is the formation water of the target reservoir.
The biological thickened oil viscosity reducer disclosed by claim 1 is mainly applied to thickened oil viscosity reduction in a stratum, wherein the oil reservoir temperature ranges from 20 ℃ to 80 ℃, and the thickened oil viscosity ranges from 1000 mPa-s to 50000 mPa-s, so that the effect of viscosity reduction in lifting or pipe transportation is achieved.
The biological thick oil viscosity reducer has the following effects:
1. the components of the biodegradable plastic are biodegradable products, and the biodegradable plastic has the characteristics of being green, non-toxic, pollution-free, harmless to stratum and the like;
2. the viscosity of the thickened oil in the stratum is reduced, and the effect of lifting or pipe transportation viscosity reduction can be achieved after treatment;
3. the pertinence is strong, and the viscosity reducing rate can reach 95 to 99 percent for different thickened oils;
4. the recovery rate of crude oil can be improved to a higher degree, and the recovery rate of crude oil can be improved by more than 14% after the biological thickened oil viscosity reducer is subjected to water flooding;
5. the method has the advantages of safety, high efficiency, low cost, long and continuous and stable effect period, higher economic benefit and wide development prospect.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
Example 1
The preparation conditions of the biological thick oil viscosity reducer are as follows: stirring at normal temperature for 30 min;
target reservoir temperature: 80 ℃;
crude oil for testing: crude oil from a certain oil field;
and (3) testing temperature: 50 ℃;
the test apparatus comprises: a Brookfield DV-II + Pro viscometer;
according to the oil reservoir conditions, functional microbial liquid is prepared, and the preparation method comprises the following steps:
a, activating a target functional strain: respectively picking out 1 ring of slant target strains (Rhodococcus erythropolis) to 100mL of liquid culture medium (500 mL conical flask) for activation culture, and performing shake culture at 80 ℃ and 120 rpm for 16-48 h to obtain activated bacterial liquid;
b, preparing a seed solution: respectively transferring the activated bacterial liquid prepared in the step a into 5L shake flasks for culture, wherein the liquid loading of the 5L shake flasks is 1.5L, and the shake culture is carried out at 80 ℃ and 120 rpm for 16-48 h to prepare seed bacterial liquid;
c, fermentation culture of first-level seeds: respectively fermenting the seed bacterial liquids prepared in the step b, introducing the fermented seed bacterial liquids into a primary seed tank (100L), culturing for 16-48 h at the temperature of 80 ℃ and with the liquid filling amount of the seed tank being 70%, the inoculation amount being 2%, and the stirring speed being 100 r/min, and the pH value being controlled to be 6.0-8.0, so as to prepare primary seed fermentation liquid;
d, fermentation culture of secondary seeds: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank (1 t), wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 r/m, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the temperature of 80 ℃;
e, fermentation tank culture: d, introducing the secondary seed solution prepared in the step d into a fermentation tank (10 t), wherein the fermentation tank is filled with 70% of liquid, the inoculation amount is 10%, the pH value in the process is controlled to be 7.0-8.0, the stirring speed is 50 rpm at the temperature of 80 ℃, and after 24-72 h of culture, a target functional strain fermentation stock solution is prepared;
f, mixing and preparing: is a single functional strain, and is a functional microbial liquid without mixing.
The liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: the components of the composition comprise 0.25 part of molasses, 0.5 part of white oil, 0.2 part of monopotassium phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.1 part of yeast powder, 0.2 part of diammonium hydrogen phosphate, 0.001 part of manganese chloride, 0.3 part of rhamnolipid and 98.30 parts of water.
The invention relates to a biological thick oil viscosity reducer, which comprises the following main components in parts by weight:
the functional microbial liquid prepared by the method comprises the following steps: 1 part;
bio-based surfactant: 0.20 part of phenyl octadecyl amido propyl dimethyl amido acetate;
5 parts of biosurfactant which is sophorolipid (lactone type);
93.8 parts of bioactivator solution; the components of the composition comprise 0.45 part of molasses, 0.3 part of white oil, 0.2 part of monopotassium phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.1 part of yeast powder, 0.2 part of diammonium hydrogen phosphate, 0.001 part of manganese chloride, 0.3 part of rhamnolipid and 98.30 parts of water.
100g of biological thick oil viscosity reducer is prepared according to the formula of the components, 30g of viscosity reducer and 70g of thick oil sample (50 ℃, 1860 mPa & s) are uniformly stirred, shake culture is carried out at 150 rpm under the oil reservoir temperature, the viscosity of crude oil is 1230 mPa & s in 1 day, the viscosity of crude oil is 460 mPa & s in 3 days, the viscosity of crude oil is 70.7 mPa & s in 7 days, and the viscosity reduction rate is 96.20 percent.
Example 2
Target reservoir temperature: 25 ℃;
the test conditions were the same as in example 1.
According to the oil reservoir conditions, functional microbial liquid is prepared, target strains are acinetobacter and rhodococcus erythropolis, and the two strains are respectively prepared into bacterial liquid according to the following methods:
a, activating a target functional strain: respectively picking out 1 ring to 100mL of liquid culture medium (500 mL of conical flask) from a slant target strain (Rhodococcus erythropolis or Acinetobacter) for activated culture, and performing shake culture at 25 ℃ and 120 rpm for 16-48 h to respectively prepare activated bacterial liquids;
b, preparing a seed solution: respectively transferring the activated bacterial liquid prepared in the step a into 5L shake flasks for culture, wherein the liquid loading of the 5L shake flasks is 1.5L, and the shake culture is carried out at 25 ℃ and 120 rpm for 16-48 h to respectively prepare bacterial liquid of each seed;
c, fermentation culture of first-level seeds: respectively fermenting the seed bacterial liquids prepared in the step b, introducing the fermented seed bacterial liquids into a primary seed tank (100L), culturing for 16-48 h at the temperature of 25 ℃ and with the liquid filling amount of the seed tank being 70%, the inoculation amount being 2%, and the stirring speed being 100 r/min, and the pH value being controlled to be 6.0-8.0, so as to prepare primary seed fermentation liquid;
d, fermentation culture of secondary seeds: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank (1 t), wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 r/m, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the temperature of 25 ℃;
e, fermentation tank culture: d, introducing the secondary seed solution prepared in the step d into a fermentation tank (10 t), wherein the fermentation tank is filled with 70% of liquid, the inoculation amount is 10%, the pH value in the process is controlled to be 7.0-8.0, the stirring speed is 50 r/min at the temperature of 25 ℃, and after culturing is carried out for 24-72 h, fermentation stock solutions of acinetobacter and rhodococcus are respectively prepared;
f, mixing and preparing: and e, respectively introducing the fermentation stock solutions of the acinetobacter and the rhodococcus erythropolis prepared in the step e into a storage tank (25 t) according to the proportion of 1:2, and stirring (50 revolutions per minute) for 30min at normal temperature to prepare the functional microbial strain solution.
The liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: the raw materials comprise 0.2 part of molasses, 0.45 part of white oil, 0.15 part of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.05 part of yeast powder, 0.18 part of diammonium hydrogen phosphate, 0.001 part of manganese chloride, 0.12 part of rhamnolipid and 98.70 parts of water.
The invention relates to a biological thickened oil viscosity reducer, which comprises the following main components in parts by weight:
functional microbial liquid: 6 parts;
bio-based surfactant: 0.20 part of phenyl octadecyl amido propyl dimethyl amido acetate;
4 parts of biosurfactant which is lipopeptide (cyclic);
89.8 parts of bioactivator solution; the components of the composition comprise 0.4 part of molasses, 0.25 part of white oil, 0.15 part of monopotassium phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.05 part of yeast powder, 0.18 part of diammonium hydrogen phosphate, 0.001 part of manganese chloride, 0.12 part of rhamnolipid and 98.70 parts of water.
100g of biological thick oil viscosity reducer is prepared according to the formula of the components, 30g of viscosity reducer and 70g of thick oil sample (50 ℃, 39600 mPa.s) are uniformly stirred, shake culture is carried out at 150 rpm under the oil reservoir temperature, the viscosity of crude oil is 25500 mPa.s in 1 day, the viscosity of crude oil is 5200 mPa.s in 3 days, the viscosity of crude oil is 1425 mPa.s in 7 days, and the viscosity reduction rate is 96.40% when measured at 50 ℃.
Example 3
Target reservoir temperature: 38 ℃;
the test conditions were the same as in example 1.
According to the oil reservoir conditions, functional microbial liquid is prepared, target strains are selected from bacillus, brevibacterium and clostridium acetobutylicum, and the three strains are respectively prepared into bacterial liquids according to the following methods:
a, activating a target functional strain: respectively picking out 1 ring to 100mL of liquid culture medium (500 mL of conical flask) from a slant target strain (bacillus or brevibacterium or clostridium acetobutylicum), and performing activation culture, and performing shake culture at 38 ℃ at 120 rpm for 16-48 h to obtain activated bacterial liquids;
b, preparing a seed solution: respectively transferring the activated bacterial liquid prepared in the step a into 5L shake flasks for culture, wherein the liquid loading of the 5L shake flasks is 1.5L, and the activated bacterial liquid is subjected to shake culture at 38 ℃ at 120 rpm for 16-48 h to prepare bacterial liquid of each seed;
c, fermentation culture of first-level seeds: respectively fermenting the seed bacterial liquid prepared in the step b, introducing the seed bacterial liquid into a first-level seed tank (100L), culturing for 16-48 h at the temperature of 38 ℃ and at the stirring speed of 100 rpm and the pH value of 6.0-8.0, wherein the liquid filling amount of the seed tank is 70%, the inoculation amount is 2%, and the first-level seed fermentation liquid is prepared;
d, fermentation culture of secondary seeds: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank (1 t), wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 rpm, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 hours at the temperature of 38 ℃;
e, fermentation tank culture: d, introducing the secondary seed solution prepared in the step d into a fermentation tank (10 t), wherein the fermentation tank is filled with 70% of liquid, the inoculation amount is 10%, the pH value in the process is controlled to be 7.0-8.0, the stirring speed is 50 rpm at 38 ℃, and after culturing for 24-72 h, fermentation stock solutions of bacillus, brevibacterium and clostridium acetobutylicum are prepared respectively;
f, mixing and preparing: and e, respectively introducing the fermentation stock solutions of the bacillus, the brevibacterium and the clostridium acetobutylicum prepared in the step e into a storage tank (25 t) according to the ratio of 3:1:2, and stirring (50 revolutions per minute) for 30min at normal temperature to prepare the functional microbial liquid.
The liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: 1.5 parts of molasses, 0.31 part of white oil, 0.3 part of monopotassium phosphate, 0.15 part of diammonium hydrogen phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.1 part of yeast powder, 0.01 part of manganese chloride, 0.1 part of rhamnolipid and 97.38 parts of water.
The invention relates to a biological thick oil viscosity reducer, which comprises the following main components in parts by weight:
the functional microbial liquid prepared by the method comprises the following steps: 10 portions of
Bio-based surfactant: 0.20 part of phenyl octadecyl amido propyl dimethyl amido acetate;
3 parts of biosurfactant, namely rhamnolipid;
86.8 parts of bioactivator solution, which comprises 1.8 parts of molasses, 0.01 part of white oil, 0.3 part of monopotassium phosphate, 0.15 part of diammonium phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.1 part of yeast powder, 0.01 part of manganese chloride, 0.1 part of rhamnolipid and 97.38 parts of water.
100g of biological thick oil viscosity reducer is prepared according to the component formula, 30g of viscosity reducer and 70g of thick oil sample (50 ℃, 28200 mPa.s) are uniformly stirred, shake culture is carried out at 150 rpm under the oil reservoir temperature, and the viscosity of crude oil is 10200 mPa.s in 1 day, 1600 mPa.s in 3 days, 248.2 mPa.s in 7 days and the viscosity reduction rate is 99.12% under the condition of 50 ℃.
Example 4
Target reservoir temperature: 65 ℃;
the test conditions were the same as in example 1.
According to the oil reservoir conditions, functional microbial liquid is prepared, target strains are selected from acinetobacter, brevibacterium, pseudomonas aeruginosa and rhodococcus erythropolis, and four strains are respectively prepared into bacterial liquid according to the following methods:
a, activating a target functional strain: respectively picking out 1 ring to 100mL of liquid culture medium (500 mL of conical flask) from a slant target strain (acinetobacter, brevibacterium, pseudomonas aeruginosa or rhodococcus erythropolis), performing activated culture, and performing shake culture at 65 ℃ and 120 rpm for 16-48 h to obtain activated bacteria solutions;
b, preparing a seed solution: respectively transferring the activated bacterial liquid prepared in the step a into 5L shake flasks for culture, wherein the liquid loading of the 5L shake flasks is 1.5L, and the shake culture is carried out at 65 ℃ and 120 rpm for 16-48 h to prepare bacterial liquid of each seed;
c, fermentation culture of first-level seeds: respectively fermenting the seed bacterial liquids prepared in the step b, introducing the fermented seed bacterial liquids into a primary seed tank (100L), culturing for 16-48 h at the temperature of 65 ℃ and the stirring speed of 100 rpm, wherein the liquid filling amount of the seed tank is 70%, the inoculation amount is 2%, and the pH value is controlled to be 6.0-8.0, so as to prepare primary seed fermentation liquid;
d, fermentation culture of secondary seeds: respectively introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank (1 t), wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 r/m, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the temperature of 65 ℃;
e, fermentation tank culture: introducing the secondary seed solution prepared in the step d into a fermentation tank (10 t), wherein the liquid loading amount of the fermentation tank is 70%, the inoculation amount is 10%, the pH value in the process is controlled to be 7.0-8.0, the stirring speed is 50 r/min at 65 ℃, and after culturing is carried out for 24-72 h, fermentation stock solutions of acinetobacter, brevibacterium, pseudomonas aeruginosa and rhodococcus erythropolis are respectively prepared;
f, mixing and preparing: and e, respectively introducing the fermentation stock solutions of the acinetobacter, the brevibacterium, the pseudomonas aeruginosa and the rhodococcus erythropolis prepared in the step e into a storage tank (25 t) according to the ratio of 1:1:1:1, and stirring (50 revolutions per minute) for 30min at normal temperature to prepare the functional microbial strain solution.
The liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: the components of the compound fertilizer are 0.1 part of molasses, 1.2 parts of white oil, 0.1 part of monopotassium phosphate, 0.2 part of diammonium hydrogen phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.05 part of yeast powder, 0.001 part of manganese chloride, 0.3 part of rhamnolipid and 97.90 parts of water.
The invention relates to a biological thick oil viscosity reducer, which comprises the following main components in parts by weight:
the functional microbial liquid prepared by the method comprises the following steps: 20 parts of (1);
bio-based surfactant: 0.30 part; is phenyl octadecyl amido propyl dimethyl amido acetate and alkyl glucoside, the proportion is 3: 1;
2 parts of biosurfactant; lipopeptide (cyclic) and sophorolipid (lactone type) in a ratio of 1: 1;
77.7 parts of bioactivator solution; the components of the compound fertilizer comprise 0.3 part of molasses, 1.0 part of white oil, 0.1 part of monopotassium phosphate, 0.2 part of diammonium hydrogen phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.05 part of yeast powder, 0.001 part of manganese chloride, 0.3 part of rhamnolipid and 97.90 parts of water.
100g of biological thick oil viscosity reducer is prepared according to the formula of the components, 30g of viscosity reducer and 70g of thick oil sample (50 ℃, 18700 mPas) are uniformly stirred, shake culture is carried out at 150 rpm under the oil reservoir temperature, the viscosity of crude oil is 9600 mPas in 1 day, the viscosity of crude oil is 3100 mPas in 3 days, the viscosity of crude oil is 267 mPas in 7 days, and the viscosity reduction rate is 98.57% under the condition of 50 ℃.
Example 5
Target reservoir temperature: 50 ℃;
the test conditions were the same as in example 1.
According to the oil reservoir conditions, functional microbial liquid is prepared, target strains are selected from acinetobacter, ochrobactrum, enterobacter cloacae, clostridium acetobutylicum and rhodococcus erythropolis, and five bacteria are respectively prepared into bacterial liquids according to the following methods:
a, activating a target functional strain: respectively picking out 1 ring to 100mL of liquid culture medium (500 mL of conical flask) from slant target strains (acinetobacter, ochrobactrum, enterobacter cloacae, clostridium acetobutylicum and rhodococcus erythropolis), and performing activated culture at 50 ℃ and 120 rpm for 16-48 h by shaking culture to obtain activated bacterial liquids;
b, preparing a seed solution: respectively transferring the activated bacterial liquid prepared in the step a into 5L shake flasks for culture, wherein the liquid loading of the 5L shake flasks is 1.5L, and the shake culture is carried out at 50 ℃ and 120 rpm for 16-48 h to prepare bacterial liquid of each seed;
c, fermentation culture of first-level seeds: respectively fermenting the seed bacterial liquids prepared in the step b, introducing the fermented seed bacterial liquids into a primary seed tank (100L), culturing for 16-48 h at 50 ℃ and at the stirring speed of 100 rpm and the pH value of 6.0-8.0, wherein the liquid filling amount of the seed tank is 70%, the inoculation amount is 2%, and the primary seed fermentation liquid is prepared;
d, fermentation culture of secondary seeds: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank (1 t), wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 r/m, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the temperature of 50 ℃;
e, fermentation tank culture: d, introducing the secondary seed solution prepared in the step d into a fermentation tank (10 t), wherein the fermentation tank contains 70% of liquid, the inoculation amount is 10%, the pH value in the process is controlled to be 7.0-8.0, the stirring speed is 50 rpm at 50 ℃, and after culturing for 24-72 h, fermentation stock solutions of acinetobacter, ochrobactrum, enterobacter cloacae, clostridium acetobutylicum and rhodococcus rhodochrous are respectively prepared;
f, mixing and preparing: and e, respectively introducing fermentation stock solutions of the acinetobacter, the ochrobactrum anthropi, the enterobacter cloacae, the clostridium acetobutylicum and the rhodococcus erythropolis prepared in the step e into a storage tank (25 t) according to the proportion of 4:3:2:2:4, and stirring (50 revolutions per minute) for 30min at normal temperature to prepare the functional microbial strain solution.
The liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: the components of the compound fertilizer are 0.4 part of molasses, 1.0 part of white oil, 0.15 part of monopotassium phosphate, 0.2 part of diammonium hydrogen phosphate, 0.05 part of magnesium sulfate, 0.1 part of sodium nitrate, 0.1 part of yeast powder, 0.005 part of manganese chloride, 0.25 part of rhamnolipid and 97.95 parts of water.
The invention relates to a biological thick oil viscosity reducer, which comprises the following main components in parts by weight:
functional microbial liquid: 15 parts of (1);
bio-based surfactant: 0.20 part; is phenyl octadecyl amido propyl dimethyl amido acetate and alkyl glucoside, the proportion is 3: 1;
5 parts of biosurfactant; lipopeptide (cyclic) and sophorolipid (lactone type) in a ratio of 2: 3;
79.8 parts of bioactivator solution; the seasoning is composed of molasses 0.6 parts, white oil 0.8 parts, potassium dihydrogen phosphate 0.15 parts, diammonium hydrogen phosphate 0.2 parts, magnesium sulfate 0.05 parts, sodium nitrate 0.1 parts, yeast powder 0.1 parts, manganese chloride 0.005 parts, rhamnolipid 0.25 parts, and water 97.95 parts.
100g of biological thick oil viscosity reducer is prepared according to the component formula, 30g of viscosity reducer and 70g of thick oil sample (50 ℃, 49500 mPa.s) are uniformly stirred, shake culture is carried out at 150 rpm under the oil reservoir temperature, the viscosity of crude oil is 29200 mPa.s in 1 day, the viscosity of crude oil is 9300 mPa.s in 3 days, the viscosity of crude oil is 381.2 mPa.s in 7 days, and the viscosity reduction rate is 99.23%.
The effects of the biological thick oil viscosity reducer prepared in examples 1 to 5 are further described below:
adopting beret core, the water phase permeability is 0.2 μm 2 And on the left and right, the displacement water is the injection water of a certain block of the treated Daqing oil field, and the displacement speed is 0.30 mL/min. The method comprises the steps of saturating water in a rock core, displacing crude oil to the saturation of bound water, aging for 48 hours at the reservoir temperature after oil saturation, displacing water to the saturation of residual oil, injecting a section plug of a 0.50 PV (PV, pore volume of a test rock core) viscosity reducer system, closing the rock core for 7 days, and finally, displacing water to the water content of 98 percent to finish the test.
TABLE 1 conditions of thickened oils for different viscosity reducer systems
The biological thick oil viscosity reducer is a microbial composite product, has the advantages of environmental protection, low cost, no damage to stratum and the like, and also has obvious advantages in the aspects of thick oil viscosity reduction, crude oil recovery rate improvement and the like. As shown in the table, the biological heavy oil viscosity reducer 1# to 5# corresponds to the formula systems of the examples 1 to 5 in sequence, and can improve the crude oil recovery by 2 to 6 percentage points compared with the conventional chemical viscosity reducers 1 and 2, and can improve the recovery by more than 15 percentage points to the maximum. Meanwhile, compared with the conventional chemical viscosity reducer system, the viscosity reduction rate of the biological thick oil viscosity reducer can be improved by more than 3 percent to reach 99.23 percent.
Claims (9)
1. The biological thick oil viscosity reducer is characterized by being prepared from the following components in parts by mass: 1-20 parts of functional microbial liquid, 1-10 parts of biosurfactant, 0.025-5 parts of bio-based surfactant and 65-97.775 parts of bio-activator solution.
2. The biological thick oil viscosity reducer according to claim 1, wherein the functional microbial liquid is prepared by fermentation culture of a target functional microbial strain and a liquid culture medium;
the preparation method of the functional microbial liquid comprises the following steps:
a. activating a target functional strain: respectively picking out 1 ring of target strains on the inclined plane to 100mL of liquid culture medium for activation culture, and performing shake culture at 120 rpm for 16-48 h at the temperature of a target oil reservoir to obtain an activated bacterial liquid;
b. preparing a seed solution: transferring the activated bacterial liquid prepared in the step a into a 5L shake flask for culture, wherein the liquid loading capacity of the 5L shake flask is 1.5L, and the seed bacterial liquid is prepared by shaking culture for 16-48 h at 120 revolutions per minute at the target oil reservoir temperature;
c. fermentation culture of first-level seeds: b, fermenting the seed bacterial liquid prepared in the step b, introducing the fermented seed bacterial liquid into a 100L primary seed tank, wherein the liquid loading amount of the seed tank is 70%, the inoculation amount is 2%, the stirring speed is 100 r/min, the pH value is controlled to be 6.0-8.0, and the primary seed fermentation liquid is prepared after culturing for 16-48 h at the oil reservoir temperature;
d. and (3) secondary seed fermentation culture: c, introducing the primary seed bacterial liquid prepared in the step c into a secondary seed tank of 1t, wherein the liquid loading amount is 70%, the inoculation amount is 10%, the stirring speed is 100 rpm, the pH value is controlled to be 6.0-8.0, and the secondary seed fermentation liquid is prepared after culturing for 16-48 h at the oil reservoir temperature;
e. culturing in a fermentation tank: d, introducing the secondary seed solution prepared in the step d into a fermentation tank of 10t, controlling the pH value of the process to be 7.0-8.0 according to 70% liquid loading amount and 10% inoculation amount in the fermentation tank, controlling the stirring speed to be 50 rpm at the oil reservoir temperature, and culturing for 24-72 h to prepare a target functional strain fermentation stock solution;
the liquid culture medium formula of the shake flask and each stage of fermentation tank comprises the following components in parts by mass: 0.1-5 parts of molasses, 0.1-5 parts of white oil, 0.01-0.3 part of yeast powder, 0.1-2 parts of monopotassium phosphate, 0.1-2 parts of diammonium phosphate, 0.001-0.05 part of manganese chloride, 0.01-0.1 part of magnesium sulfate, 0.1-3 parts of sodium nitrate, 0.1-2 parts of rhamnolipid and 80.55-99.38 parts of water.
3. The bio-thick oil viscosity reducer of claim 2, wherein said target functional microbial species comprises one or more of Acinetobacter, Brevibacterium, Bacillus, Enterobacter cloacae, Ochrobactrum, Clostridium acetobutylicum, Pseudomonas aeruginosa, Rhodococcus erythropolis.
4. The functional microbial cell according to claim 2, wherein said target functional microbial species may be a single species or a plurality of species.
5. The bio-thick oil viscosity reducer according to claim 1, wherein the biosurfactant is one or more of lipopeptide, rhamnolipid and sophorolipid in any ratio.
6. The bio-thick oil viscosity reducer according to claim 1, wherein the bio-based surfactant is one or more of phenyl stearamidopropyl dimethyl aminoacetate, alkyl glycoside, sucrose fatty acid ester and glycerol mono fatty acid ester in any ratio.
7. The bio-thick oil viscosity reducer according to claim 1, wherein the bio-activator solution comprises, by mass, 0.1 to 5 parts of molasses, 0.01 to 5 parts of white oil, 0.01 to 0.3 part of yeast powder, 0.1 to 2 parts of monopotassium phosphate, 0.1 to 2 parts of diammonium phosphate, 0.001 to 0.05 part of manganese chloride, 0.01 to 0.1 part of magnesium sulfate, 0.1 to 3 parts of sodium nitrate, 0.1 to 2 parts of rhamnolipid, and 80.55 to 99.47 parts of water.
8. The bio-heavy oil viscosity reducer of claim 1, wherein the water is formation water of a target reservoir.
9. The application of the biological thick oil viscosity reducer as claimed in claim 1, wherein the biological thick oil viscosity reducer is mainly applied to a thick oil viscosity reducer in a stratum, the oil deposit temperature range is 20-80 ℃, and the thick oil viscosity range is 1000-50000 mPa-s, so that the effect of lifting or pipe transportation viscosity reduction is achieved.
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| CN107312515A (en) * | 2017-06-01 | 2017-11-03 | 大庆华理生物技术有限公司 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
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| CN107312515A (en) * | 2017-06-01 | 2017-11-03 | 大庆华理生物技术有限公司 | A kind of multi-element biologic compound oil displacement agent system and its injection technology |
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| CN116355603B (en) * | 2023-03-21 | 2024-04-16 | 陕西延长石油（集团）有限责任公司 | Non-ultralow interfacial tension oil displacement agent for improving recovery ratio of low-permeability oil reservoir as well as preparation method and application thereof |
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