CN114835523B - Medium trace element fertilizer and preparation method and application thereof - Google Patents
Medium trace element fertilizer and preparation method and application thereof Download PDFInfo
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- CN114835523B CN114835523B CN202210423885.0A CN202210423885A CN114835523B CN 114835523 B CN114835523 B CN 114835523B CN 202210423885 A CN202210423885 A CN 202210423885A CN 114835523 B CN114835523 B CN 114835523B
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- 239000011573 trace mineral Substances 0.000 title claims abstract description 57
- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 57
- 239000003337 fertilizer Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims abstract description 31
- KWSUGULOZFMUDH-UHFFFAOYSA-N 2-amino-3-methylhexanoic acid Chemical compound CCCC(C)C(N)C(O)=O KWSUGULOZFMUDH-UHFFFAOYSA-N 0.000 claims abstract description 28
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 26
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 17
- 235000015393 sodium molybdate Nutrition 0.000 claims description 16
- 239000011684 sodium molybdate Substances 0.000 claims description 16
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 13
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 13
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 12
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 12
- 239000011575 calcium Substances 0.000 claims description 12
- 229910052791 calcium Inorganic materials 0.000 claims description 12
- 240000000560 Citrus x paradisi Species 0.000 claims description 8
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 150000002596 lactones Chemical class 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 10
- 238000011160 research Methods 0.000 abstract description 9
- 238000003306 harvesting Methods 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 27
- 238000011282 treatment Methods 0.000 description 20
- 230000000052 comparative effect Effects 0.000 description 16
- 241000219095 Vitis Species 0.000 description 13
- 235000009754 Vitis X bourquina Nutrition 0.000 description 12
- 235000012333 Vitis X labruscana Nutrition 0.000 description 12
- 235000014787 Vitis vinifera Nutrition 0.000 description 12
- 229930002875 chlorophyll Natural products 0.000 description 12
- 235000019804 chlorophyll Nutrition 0.000 description 12
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 12
- 235000013399 edible fruits Nutrition 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 239000011259 mixed solution Substances 0.000 description 6
- 238000005507 spraying Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 5
- 239000004327 boric acid Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- -1 amino acid compound Chemical class 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000003973 paint Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000010338 boric acid Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000007180 physiological regulation Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000026267 regulation of growth Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
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- 239000012086 standard solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000004347 surface barrier Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of agricultural special functional fertilizers, and particularly relates to a medium trace element fertilizer which comprises the following raw materials in percentage by weight: 2-8% of medium elements, 1-6% of trace elements, 0.5-2% of sophorolipid, 0.01-0.1% of 2-amino-3-methylhexanoic acid and 100% of water. The invention also relates to a preparation method and application of the medium trace element fertilizer. The research result proves that the synergistic effect can be generated in the aspects of improving the physiological and biochemical properties of plants and improving the quality of plant harvest by combining the sophorolipid and the 2-amino-3-methylhexanoic acid. In addition, the invention combines sophorolipid and 2-amino-3-methyl caproic acid, and further mixes and applies the sophorolipid and the 2-amino-3-methyl caproic acid with medium trace elements, and the two components are mutually promoted in effect.
Description
Technical field:
the invention belongs to the field of agricultural special functional fertilizers, and particularly relates to a medium trace element fertilizer, a preparation method and application thereof.
The background technology is as follows:
early fertilization methods met plant growth requirements mainly by applying a base fertilizer. Along with the development of scientific planting and reasonable fertilization technology, foliar fertilization is becoming more and more important as a refined fertilization mode. Compared with a base fertilizer application mode, the foliar fertilizer has various advantages, such as avoiding unnecessary soil adsorption, slowing down or reducing the soil hardening degree, improving the utilization rate of nutrient elements and the like, and various foliar fertilizer products aiming at various crops are available in the market at present. However, while foliar application has many of the advantages described above, there are also application problems, such as how to encourage nutrient elements to pass through the plant leaf surface barrier for rapid and efficient uptake by plants, and so on.
Sophorolipids were first discovered from microbial fermentation products and were initially used primarily as biosurfactants for oil exploitation and product processing as environmentally compatible materials. With the continuous and intensive understanding of people, more and more researches find that sophorolipids have better effects in various aspects of medical anti-inflammatory, disinfection, agricultural antibacterial, growth regulation and the like.
2-amino-3-methylhexanoic acid belongs to an amino acid compound, which was originally isolated from a microorganism. Early researches mainly focused on the aspect of inhibiting the growth of bacteria, for example, researches show that the compound has a certain inhibition effect on escherichia coli, and researches on the compound are increased in recent years, wherein the researches comprise biological fermentation synthesis, chemical synthesis, expansion of application fields and the like, and research teams initially confirm the application value of the 2-amino-3-methylhexanoic acid in agriculture, for example, in the aspects of disease control and plant resistance induction, but in general, the early research stage still exists, and a large space exists for exploring the compound in various aspects.
Disclosure of Invention
The inventor discovers that the sophorolipid and the 2-amino-3-methyl caproic acid have certain plant physiological regulation effect, and the two are combined to generate synergistic effect, so that the physiological and biochemical properties of plants can be effectively improved, and the quality of plant harvest can be improved.
Based on the discovery, the inventor combines the fertilizer with medium trace element fertilizer to prepare foliar fertilizer, which not only can improve plant properties, but also can increase foliar absorption efficiency of the medium trace element due to the existence of sophorolipid and the like, promote plant growth and development, and further enhance interaction synergistic effect between the medium trace element, sophorolipid and 2-amino-3-methyl caproic acid.
The invention relates to a medium trace element fertilizer, which comprises the following raw materials in percentage by weight:
medium element: 2-8%
Trace elements: 1-6%
Sophorolipids: 0.5-2%
2-amino-3-methylhexanoic acid: 0.01-0.1%
Water: the complement is 100 percent.
In the medium trace element fertiliser according to the invention, the weight ratio of sophorolipids to 2-amino-3-methylhexanoic acid is from 10:1 to 100:1, preferably from 20:1 to 50:1, such as 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1.
In the medium trace element fertilizer, the medium element comprises one or two of magnesium sulfate and EDTA chelated calcium, preferably a combination of magnesium sulfate and EDTA chelated calcium, and the weight ratio of the magnesium sulfate to the EDTA chelated calcium is controlled to be in a range of 1:3 to 3:1.
In the medium trace element fertilizer, the trace elements comprise one or more of boric acid, sodium molybdate and zinc sulfate heptahydrate, and the weight ratio of the boric acid, the sodium molybdate and the zinc sulfate heptahydrate is controlled to be in the range of 0-5:0-5:0-5, wherein the three are not 0 at the same time. Preferably, the trace elements include at least two of them, such as boric acid and sodium molybdate, sodium molybdate and zinc sulfate heptahydrate, boric acid and zinc sulfate heptahydrate, or the trace elements include the three of them, namely boric acid and sodium molybdate and zinc sulfate heptahydrate. More preferably, the trace elements are a combination of sodium molybdate and zinc sulfate heptahydrate, and the weight ratio of the sodium molybdate to the zinc sulfate heptahydrate is controlled to be in the range of 1:2 to 2:1.
In one embodiment of the present invention, the medium trace element fertilizer preferably comprises the following raw materials in percentage by weight:
medium element: 4-6%
Trace elements: 2-4%
Sophorolipids: 0.8-1.6%
2-amino-3-methylhexanoic acid: 0.04 to 0.08 percent
Water: the complement is 100 percent.
In the present invention, the sophorolipid is lactone type sophorolipid (LSL, CAS registry number is 148409-20-5); the 2-amino-3-methylhexanoic acid (2-amino-3-methylhexanoic acid, CAS registry number 60182-96-9) belongs to amino acid compounds. Both of which are commercially available. Studies have shown that sophorolipids and 2-amino-3-methylhexanoic acid can be applied to a variety of fields, have various effects, such as plant disease control in agriculture, induction of plant resistance, etc., and the search for new fields of application of both is continuously underway. The inventor proves that the sophorolipid and the 2-amino-3-methylhexanoic acid have application prospects in improving the physiological and biochemical properties of plants and improving the quality of plant harvest. In particular, the two are used for grapes, the chlorophyll content of grape leaves can be increased, the total sugar content in grape fruits can be increased, and when the two are used in combination, a synergistic effect can be generated. In addition, the inventor also proves that the foliar fertilizer prepared by combining the two with the medium trace element fertilizer is foliar fertilizer, and foliar spraying can also increase the foliar absorption efficiency of the medium trace element, which is probably due to the surface activity of sophorolipid, improves the penetrability of the plant surface, is beneficial to the absorption of nutrient elements, is beneficial to the exertion of the sophorolipid and the 2-amino-3-methylhexanoic acid, and further enhances the interaction synergistic effect between the medium trace element, the sophorolipid and the 2-amino-3-methylhexanoic acid.
The invention also relates to a preparation method of the medium trace element fertilizer, which comprises the following steps:
1) Taking a proper amount of water, and then sequentially dissolving the formula amount of medium elements and trace elements into the water to prepare a solution form to obtain a mixed solution I;
2) Adding sophorolipid with the formula amount into the mixed solution I prepared in the step 1) and uniformly mixing to obtain a mixed solution II;
3) Adding 2-amino-3-methylhexanoic acid with the formula amount into the mixed solution II prepared in the step 2) and uniformly mixing to obtain a mixed solution III;
4) Adding the rest water into the mixed solution III prepared in the step 3) and uniformly mixing.
In the process according to the invention, the water consumption in step 1) is 20 to 80%, preferably 30 to 60%, of the total amount of formulation water.
In a further aspect the invention relates to the use of said medium trace element fertiliser, wherein the use is primarily for improving the physiological and biochemical properties of plants and/or the quality of the harvest. The plants include field crops, vegetable crops, fruit trees and the like, and the preferred plant is grape. The plant physiological and biochemical shape comprises improvement of plant dry weight and fresh weight, improvement of rhizome ratio, improvement of leaf shape and the like, and particularly, the medium trace element fertilizer can increase the chlorophyll content of plant leaves. The harvest quality comprises appearance quality, such as increasing seed fullness, fruit size and the like, and improvement of fruit biochemical indexes, such as nutrition component increase, taste improvement and the like, and in particular, the medium trace element fertilizer can increase the content of soluble total sugar in the plant harvest.
The application mode of the medium trace elements is not particularly limited, and comprises root irrigation, leaf surface spraying and the like. The preferable application mode is blade spraying, for example, the medium trace elements are diluted by 50-400 times by adding water when being applied, and are sprayed on the plant surface. The specific spraying amount can be adjusted according to different plants.
Preferably, the invention relates to the use of said medium trace element fertilizer for increasing the chlorophyll content of grape leaves, the method and the dosage of which are as described above.
Further preferred, the invention relates to the use of said medium trace element fertilizer for increasing the soluble total sugar content of grape fruits, the method and dosage of which are as described above.
The weight percentages of the components are weight percentages unless otherwise specified.
The invention has the beneficial effects that:
the research result proves that the synergistic effect can be generated in the aspects of improving the physiological and biochemical properties of plants and improving the quality of plant harvest by combining the sophorolipid and the 2-amino-3-methylhexanoic acid. Specifically, the combination of the two can increase the chlorophyll content of grape leaves and increase the total sugar content of grape fruits.
In addition, the invention combines sophorolipid and 2-amino-3-methyl caproic acid, and further mixes and applies the sophorolipid and the 2-amino-3-methyl caproic acid with medium trace elements, and the two components are mutually promoted in effect. Specifically, the presence of sophorolipid and 2-amino-3-methylhexanoic acid can increase the foliar absorption efficiency of medium and trace elements; in addition, the medium trace elements are used as beneficial elements, and the efficacy of sophorolipid and 2-amino-3-methylhexanoic acid in improving the physiological and biochemical properties of plants and the quality of harvest products can be further improved.
Drawings
FIG. 1 is a graph showing the effect of each treatment on chlorophyll content of grape leaves
FIG. 2 is a graph showing the effect of each treatment on the soluble total sugar content of grape fruits
Detailed Description
The following examples are provided to further illustrate the invention and are not to be construed as limiting the invention in any way.
The raw materials used in the following examples of the present invention are all commercially available.
1. Preparation example
Example 1
The formula of the medium trace element fertilizer comprises the following components: 30g of magnesium sulfate, 20g of sodium molybdate, 30g of zinc sulfate heptahydrate, 10g of sophorolipid, 0.8g of 2-amino-3-methylhexanoic acid and 1000g of water.
The preparation method comprises the following steps: weighing water accounting for 50% of the total amount of the formula, sequentially adding the medium elements and the trace elements in the formula amount, and stirring for dissolution. Then adding the sophorolipid with the formula amount and uniformly mixing; then adding 2-amino-3-methylhexanoic acid with the formula amount and uniformly mixing; and finally, adding the rest water and uniformly mixing to prepare the medium trace element fertilizer.
Example 2
The formula of the medium trace element fertilizer comprises the following components: 10g of magnesium sulfate, 30g of EDTA chelated calcium, 20g of sodium molybdate, 20g of zinc sulfate heptahydrate, 12g of sophorolipid, 0.6g of 2-amino-3-methylhexanoic acid and 1000g of water.
The preparation method is the same as in example 1.
Example 3
The formula of the medium trace element fertilizer comprises the following components: 25g of magnesium sulfate, 25g of EDTA chelated calcium, 10g of sodium molybdate, 20g of zinc sulfate heptahydrate, 14g of sophorolipid, 0.4g of 2-amino-3-methylhexanoic acid and 1000g of water.
The preparation method is the same as in example 1.
Example 4
The formula of the medium trace element fertilizer comprises the following components: 40g of magnesium sulfate, 20g of EDTA chelated calcium, 10g of sodium molybdate, 10g of zinc sulfate heptahydrate, 16g of sophorolipid, 0.32g of 2-amino-3-methylhexanoic acid and 1000g of water.
The preparation method is the same as in example 1.
Example 5
The formula of the medium trace element fertilizer comprises the following components: 30g of magnesium sulfate, 40g of EDTA chelated calcium, 10g of zinc sulfate heptahydrate, 20g of sophorolipid, 0.2g of 2-amino-3-methyl caproic acid and 1000g of water.
The preparation method is the same as in example 1.
Comparative example 1
The formula comprises the following components: 25g of magnesium sulfate, 25g of EDTA chelated calcium, 10g of sodium molybdate, 20g of zinc sulfate heptahydrate and 1000g of water.
The preparation method comprises the following steps: weighing water accounting for 50% of the total amount of the formula, sequentially adding the medium elements and the trace elements in the formula amount, and stirring for dissolution. Then adding the rest water and mixing uniformly.
Comparative example 2
The formula comprises the following components: 25g of magnesium sulfate, 25g of EDTA chelated calcium, 10g of sodium molybdate, 20g of zinc sulfate heptahydrate, 28g of sophorolipid and 1000g of water.
The preparation method comprises the following steps: weighing water accounting for 50% of the total amount of the formula, sequentially adding the medium elements and the trace elements in the formula amount, and stirring for dissolution. Then adding the sophorolipid with the formula amount and uniformly mixing; and finally, adding the rest amount of water and uniformly mixing to obtain the water-based paint.
Comparative example 3
The formula comprises the following components: 25g of magnesium sulfate, 25g of EDTA chelated calcium, 10g of sodium molybdate, 20g of zinc sulfate heptahydrate, 0.8g of 2-amino-3-methyl caproic acid and 1000g of water.
The preparation method comprises the following steps: weighing water accounting for 50% of the total amount of the formula, sequentially adding the medium elements and the trace elements in the formula amount, and stirring for dissolution. Then adding 2-amino-3-methylhexanoic acid with the formula amount and uniformly mixing; and finally, adding the rest amount of water and uniformly mixing to obtain the water-based paint.
2. Biological assay
Test plants: grape is red earth.
Test agent: medium trace element fertilizers described in examples 1 to 5 and comparative examples 1 to 3.
The test is carried out on a grape planting base in Xinjiang, which is 5-year-old grape, the plant row spacing is 0.8mX3m, and the facility cultivation is conventionally managed. The test is carried out by 8 treatments, each treatment is repeated three times, 24 cells are divided, each cell is arranged randomly, and 50 strains are arranged in each cell. In the test, the test agent is diluted by 200 times by adding water and respectively sprayed once in the leaf spreading period, the pre-flowering period and the young fruit period for three times. The spraying amount of each treatment is the same and is limited by blade dropping.
1. Chlorophyll content determination:
and on the 15 th day after the third spraying, selecting grape leaves with consistent growth conditions of 3-4 sections of basal parts from grape plants which have no plant diseases and insect pests and are normal in each cell row according to a scattered random sampling rule, measuring chlorophyll content by using a SPAD-502 type chlorophyll meter, measuring 10 leaves per cell, measuring 30 leaves per treatment, and taking an average value. The measurement results are shown in Table 1 and FIG. 1.
TABLE 1 Effect of treatments on chlorophyll content of grape leaves
Numbering device | Treatment of | Chlorophyll content (SPAD value) |
1 | Example 1 | 43.14 |
2 | Example 2 | 46.88 |
3 | Example 3 | 48.62 |
4 | Example 4 | 45.36 |
5 | Example 5 | 42.27 |
6 | Comparative example 1 | 36.05 |
7 | Comparative example 2 | 38.72 |
8 | Comparative example 3 | 40.11 |
The results in Table 1 show that the treatments of examples 1-5 of the present invention significantly increased the chlorophyll content of the grape leaf compared to comparative example 1 containing only medium trace elements, comparative example 2 containing medium trace elements and sophorolipids, and comparative example 3 containing medium trace elements and 2-amino-3-methylhexanoic acid. Further, comparing the results of the treatments of examples 1 to 5 of the present invention, the results show that the treatments of examples 2 to 4 are more remarkable in the degree of improvement of chlorophyll content, especially the treatment of example 3.
2. Determination of soluble total sugar content:
and (3) adopting an anthrone-sulfuric acid colorimetric method, randomly selecting 10 grape fruits with more consistent growth vigor in each cell according to a random dispersion sampling rule in the ripening period of the grape, crushing and uniformly mixing fresh pulp with peel and seeds removed, taking 1g of crushed fruit sample, adding 20mL of 80% ethanol solution, extracting for 30min by using a high-speed homogenizer, separating the heart for 10min at 5000 revolutions per minute, and fixing the volume of the supernatant to 100mL to obtain the total sugar extract. Adding 4mL of anthrone reagent (prepared by dissolving 0.2g of anthrone in 100mL of 80% (V/V) sulfuric acid, which is prepared at present) into 1mL of the extracting solution, carrying out boiling water bath for 10min, taking out, rapidly cooling to room temperature by using a room temperature water bath, measuring the light absorption value of a sample at 620nm by using a spectrophotometer, calculating the soluble total sugar content of the sample according to a standard curve (prepared by using a glucose standard solution), and calculating the soluble total sugar content in fruits. The results of each treatment area are averaged to obtain the total sugar content of the treatment area. The measurement results are shown in Table 2 and FIG. 2.
TABLE 2 influence of treatments on the soluble total sugar content of grape fruits
Numbering device | Treatment of | Soluble total sugar (mg/g FW) |
1 | Example 1 | 205.93 |
2 | Example 2 | 221.72 |
3 | Example 3 | 243.38 |
4 | Example 4 | 226.13 |
5 | Example 5 | 211.58 |
6 | Comparative example 1 | 168.45 |
7 | Comparative example 2 | 177.22 |
8 | Comparative example 3 | 184.71 |
The results in Table 2 show that the treatment of examples 1-5 according to the present invention significantly increased the total soluble sugar content in the grape fruits compared to comparative example 1 containing only medium trace elements, comparative example 2 containing medium trace elements and sophorolipids, and comparative example 3 containing medium trace elements and 2-amino-3-methylhexanoic acid. Further, comparing the results of the treatments of examples 1-5 of the present invention, the results show that the treatments of examples 2-4 provide a more pronounced increase in soluble total sugar content, especially the treatment of example 3.
The advantages of the present invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the foregoing embodiments, and that the foregoing embodiments and descriptions are merely illustrative of the invention, and that any changes and modifications may be made without departing from the spirit and scope of the invention as claimed.
Claims (2)
1. The application of the medium trace element fertilizer to increasing the content of soluble total sugar in grape fruits is characterized in that,
the formula of the medium trace element fertilizer comprises the following components:
magnesium sulfate 25g, EDTA chelated calcium 25g, sodium molybdate 10g, zinc sulfate heptahydrate 20g, sophorolipid 14g, 2-amino-3-methylhexanoic acid 0.4g, water make up 1000g;
the sophorolipid is lactone type sophorolipid.
2. The use according to claim 1, characterized in that the preparation method of the medium trace element fertilizer comprises the following steps:
weighing water accounting for 50% of the total amount of the formula, sequentially adding the medium elements and the trace elements in the formula amount, and stirring for dissolution; the medium element is magnesium sulfate and EDTA chelated calcium; the microelements are sodium molybdate and zinc sulfate heptahydrate; then adding the sophorolipid with the formula amount and uniformly mixing; then adding 2-amino-3-methylhexanoic acid with the formula amount and uniformly mixing; finally, the remaining amount of water was added and mixed well.
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