CN114831305A - Method for preparing hop by modification - Google Patents
Method for preparing hop by modification Download PDFInfo
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- CN114831305A CN114831305A CN202210414280.5A CN202210414280A CN114831305A CN 114831305 A CN114831305 A CN 114831305A CN 202210414280 A CN202210414280 A CN 202210414280A CN 114831305 A CN114831305 A CN 114831305A
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- cannabidiol
- hop
- hops
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- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000004048 modification Effects 0.000 title abstract description 8
- 238000012986 modification Methods 0.000 title abstract description 8
- QHMBSVQNZZTUGM-UHFFFAOYSA-N Trans-Cannabidiol Natural products OC1=CC(CCCCC)=CC(O)=C1C1C(C(C)=C)CCC(C)=C1 QHMBSVQNZZTUGM-UHFFFAOYSA-N 0.000 claims abstract description 50
- QHMBSVQNZZTUGM-ZWKOTPCHSA-N cannabidiol Chemical compound OC1=CC(CCCCC)=CC(O)=C1[C@H]1[C@H](C(C)=C)CCC(C)=C1 QHMBSVQNZZTUGM-ZWKOTPCHSA-N 0.000 claims abstract description 50
- 229950011318 cannabidiol Drugs 0.000 claims abstract description 50
- ZTGXAWYVTLUPDT-UHFFFAOYSA-N cannabidiol Natural products OC1=CC(CCCCC)=CC(O)=C1C1C(C(C)=C)CC=C(C)C1 ZTGXAWYVTLUPDT-UHFFFAOYSA-N 0.000 claims abstract description 50
- PCXRACLQFPRCBB-ZWKOTPCHSA-N dihydrocannabidiol Natural products OC1=CC(CCCCC)=CC(O)=C1[C@H]1[C@H](C(C)C)CCC(C)=C1 PCXRACLQFPRCBB-ZWKOTPCHSA-N 0.000 claims abstract description 45
- 235000008694 Humulus lupulus Nutrition 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 108010059892 Cellulase Proteins 0.000 claims abstract description 10
- 229940106157 cellulase Drugs 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000004094 surface-active agent Substances 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229940068968 polysorbate 80 Drugs 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- 229940093499 ethyl acetate Drugs 0.000 claims description 2
- 239000011736 potassium bicarbonate Substances 0.000 claims description 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 2
- 235000015497 potassium bicarbonate Nutrition 0.000 claims description 2
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 2
- 235000011181 potassium carbonates Nutrition 0.000 claims description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 235000017550 sodium carbonate Nutrition 0.000 claims description 2
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 2
- 230000003115 biocidal effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000009920 chelation Effects 0.000 abstract 1
- 235000013405 beer Nutrition 0.000 description 8
- 238000010829 isocratic elution Methods 0.000 description 5
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 5
- 239000013558 reference substance Substances 0.000 description 5
- VMSLCPKYRPDHLN-UHFFFAOYSA-N (R)-Humulone Chemical compound CC(C)CC(=O)C1=C(O)C(CC=C(C)C)=C(O)C(O)(CC=C(C)C)C1=O VMSLCPKYRPDHLN-UHFFFAOYSA-N 0.000 description 2
- QRDZSRWEULKVNW-UHFFFAOYSA-N 6-hydroxy-2-oxo-1h-quinoline-4-carboxylic acid Chemical compound C1=C(O)C=C2C(C(=O)O)=CC(=O)NC2=C1 QRDZSRWEULKVNW-UHFFFAOYSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 201000006585 gastric adenocarcinoma Diseases 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000004410 intraocular pressure Effects 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a method for preparing modified hops, which adopts a chelation method, and comprises the steps of ultrasonically dissolving dried hop powder, cellulase and ethanol, keeping the temperature at 50-60 ℃ for 1-4h, ultrasonically dissolving cannabidiol, a surfactant and pure water, adding the dissolved solution into the solution, ultrasonically mixing the solution at normal pressure in an ultrasonic mixer, and concentrating and drying the solution under reduced pressure to obtain hops containing active cannabidiol; the method effectively improves the active factors of the hops, introduces the cannabidiol modified substances, ensures that the hops after modification have the effects of antibiosis, antiphlogosis, pressure resistance and the like, has better development prospect, and is easy to realize industrial production.
Description
Technical Field
The invention belongs to the field of food and health care products, and particularly relates to a hop modification preparation method containing active cannabidiol.
Background
The alcohol blossom is known as the spirit of beer and becomes one of the indispensable raw materials for brewing beer. Hops can not only serve as a natural preservative in beer, but also impart unique bitterness and aroma characteristics to beer. The quality or modification of hops directly affects the quality of beer, wherein the resinous components, volatile oils and polyphenols affect the taste and flavor of the beer. The hop has strong medicinal value: the alpha-acid and the beta-acid in the hop have good antibacterial action; the oxidation resistance is realized, and alpha-acid and hydrogenation products thereof, beta-acid and polyphenol substances in the hop have the oxidation resistance; the resin component in flos Lupuli has antitumor effect, and can inhibit human gastric adenocarcinoma cell CRL-1793 and mastadenoma cell-A-427; has the protective effect on the cardiovascular system, and quercetin, rutin and the like in the hops can prevent blood platelets from aggregating, thereby playing the roles of anticoagulation and blood vessel protection. Therefore, the modification of hops is a major research and development direction in this field.
Cannabidiol has the effects of analgesia, intraocular pressure reduction, antibiosis, anti-inflammation, thrombus resistance and the like, and has strong potential application value in the fields of medicine, food and the like.
According to the invention, cannabidiol is added into the hops, so that the content of phenols in the hops is effectively increased, and the hop is an ideal addition mode, so that more modification space is provided for beer brewing.
The same literature disclosures as the technical scheme of the invention are not found at present.
Disclosure of Invention
The invention aims to provide a preparation method for hop modification, which enriches the functionalization of hops in beer, cannabidiol is an important beneficial component, the added value of materials is improved through deep processing, and the preparation method has obvious significance for national economic construction, but cannabidiol is not easy to be comprehensively utilized, so how to comprehensively utilize cannabidiol and improve the application performance of cannabidiol is one of important research points; the invention prepares the hop containing the active cannabidiol by modifying the hop, and effectively combines the cannabidiol and the hop.
The preparation method of the hop containing the active cannabidiol comprises the following steps:
(1) ultrasonically dispersing dried hop powder and cellulase in ethanol, and keeping the water bath at the temperature of 50-60 ℃ for 1-4 h;
the mass ratio of the hop dry powder to the ethanol is 1:5-1:20, and the addition amount of the cellulase is 0.5% -5% of the mass of the hop dry powder.
The cellulase is one or more of exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and xylanase;
(2) mixing cannabidiol, surfactant and pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersed liquid into the mixture obtained in the step (1), stirring to uniformly mix the dispersed liquid, and adjusting the pH value of the mixture to 7-8;
the surfactant is one or more of polyethylene glycol, sodium dodecyl benzene sulfonate, polysorbate-80 and ethyl acetate, the addition amount of the surfactant is 0.05-1% of the mass of the dried hop powder, and the addition amount of the cannabidiol is 0.1-1% of the mass of the dried hop powder.
The pH regulator is one or more of sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, potassium carbonate and potassium bicarbonate, and the quality of the pure water is equal to that of the dried hop powder in the step 1.
(3) Ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer at normal pressure for 30-60 min;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, controlling the temperature at 40-75 ℃, and concentrating and drying under reduced pressure to obtain the hop containing the active cannabidiol.
Using a chromatographic column: InertSustain C18, isocratic elution with methanol-water (85: 15) as the mobile phase. Under the chromatographic condition, a CBD reference substance and a hop sample containing active cannabidiol are respectively injected and analyzed.
Compared with the prior art, the invention has the following advantages:
(1) the preparation method is simple, low in cost and mild in reaction condition;
(2) the cannabidiol is added in a milder reaction mode without damaging the active ingredients of the hop material;
(3) the raw materials adopted by the invention are environment-friendly, the process is simple, and the industrial production is easy to realize.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the scope of the present invention is not limited to the above-mentioned descriptions.
Example 1:
(1) ultrasonically dispersing 100g of hop dry powder and 0.5g of cellulase in 500g of ethanol, and then keeping the constant temperature of a water bath at 50 ℃ for 3 hours;
(2) mixing 0.1g of cannabidiol, 0.05g of polyethylene glycol and 100g of pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersed liquid into the mixture obtained in the step (1), stirring to uniformly mix the dispersed liquid and the mixture, and adjusting the pH value of the mixture to 7 by adopting sodium carbonate;
(3) ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer at normal pressure for 30 min;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, controlling the temperature at 60 ℃, and performing reduced pressure concentration and drying to obtain the hop containing the active cannabidiol.
Hops of active cannabidiol prepared in this example were purified using a chromatographic column: InertSustain C18, and performing isocratic elution by using methanol-water (volume ratio is 85: 15) as a mobile phase; under the chromatographic condition, a CBD reference substance and a hop sample containing active cannabidiol are respectively injected and analyzed. The result shows that the hop of the active cannabidiol contains 350mg/kg of the active cannabidiol.
Example 2:
(1) dispersing 200g of hop dry powder and 2g of cellulase in 500g of ethanol by ultrasonic, and keeping the water bath at the temperature of 60 ℃ for 2 hours;
(2) mixing 2g of cannabidiol, 0.16g of polysorbate-80 and 200g of pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersion liquid into the mixture obtained in the step (1), stirring to uniformly mix the mixture, and adjusting the pH value of the mixture to 8 by using sodium bicarbonate;
(3) ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer at normal pressure for 60 min;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, controlling the temperature at 50 ℃, and concentrating and drying under reduced pressure to obtain the hop containing the active cannabidiol.
Hops of active cannabidiol prepared in this example were purified using a chromatographic column: InertSustain C18, and performing isocratic elution by using methanol-water (85: 15) as a mobile phase; under the chromatographic condition, the CBD reference substance and the hop sample containing the active cannabidiol are respectively injected and analyzed, and the result shows that the hop containing the active cannabidiol has the active cannabidiol content of 4200 mg/kg.
Example 3:
(1) dispersing 300g of hop dry powder and 6g of cellulase in 500g of ethanol by ultrasonic, and then keeping the constant temperature of 55 ℃ water bath for 2 h;
(2) mixing 1.5g of cannabidiol, 3g of sodium dodecyl benzene sulfonate and 300g of pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersed liquid into the mixture obtained in the step (1), stirring to uniformly mix the dispersed liquid and the mixture, and adjusting the pH value of the mixture to 7 by using sodium hydroxide;
(3) ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer at normal pressure for 40 min;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, controlling the temperature at 60 ℃, and carrying out reduced pressure concentration and drying to obtain the hop containing the active cannabidiol.
Hops of active cannabidiol prepared in this example were purified using a chromatographic column: InertSustain C18, isocratic elution with methanol-water (85: 15) as the mobile phase. Under the chromatographic condition, a CBD reference substance and a hop sample containing the active cannabidiol are taken for sample injection analysis respectively, and the result shows that the hop containing the active cannabidiol has the active cannabidiol content of 1800 mg/kg.
Example 4:
(1) dispersing 400g of hop dry powder and 10g of cellulase in 500g of ethanol by ultrasonic, and keeping the constant temperature of 55 ℃ water bath for 3 h;
(2) mixing 2g of cannabidiol, 0.05g of sodium dodecyl benzene sulfonate and 400g of pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersed liquid into the mixture obtained in the step (1), stirring to uniformly mix the dispersed liquid and the mixture, and adjusting the pH value of the mixture to 7 by using sodium carbonate;
(3) ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer for 50min at normal pressure;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, controlling the temperature at 40 ℃, and concentrating and drying under reduced pressure to obtain the hop containing the active cannabidiol.
Hops of active cannabidiol prepared in this example were purified using a chromatographic column: InertSustain C18, and performing isocratic elution by using methanol-water (85: 15) as a mobile phase; under the chromatographic condition, a CBD reference substance and a hop sample containing active cannabidiol are respectively injected and analyzed. The result shows that the hop of the active cannabidiol contains 1800mg/kg of the active cannabidiol.
Claims (5)
1. A method for modifying and preparing hop is characterized by comprising the following specific steps:
(1) ultrasonically dispersing dried hop powder and cellulase in ethanol, and keeping the water bath at the temperature of 50-60 ℃ for 1-4 h;
(2) mixing cannabidiol, surfactant and pure water, performing ultrasonic treatment until the mixture is uniformly dispersed, dropwise adding the dispersed liquid into the mixture obtained in the step (1), stirring to uniformly mix the dispersed liquid, and adjusting the pH value of the mixture to 7-8;
(3) ultrasonically mixing the mixture obtained in the step (2) in an ultrasonic mixer at normal pressure for 30-60 min;
(4) and (4) transferring the mixture obtained in the step (3) to a multi-effect external circulation evaporator, and performing reduced pressure concentration and drying to obtain the hop containing the active cannabidiol.
2. The method for the modified preparation of hops according to claim 1, characterized in that: the addition amount of the cellulase is 0.5-5% of the mass of the dried hop powder.
3. The method for the modified preparation of hops according to claim 1, characterized in that: the surfactant is one or more of polyethylene glycol, sodium dodecyl benzene sulfonate, polysorbate-80 and ethyl acetate, the addition amount of the surfactant is 0.05-1% of the mass of the dried hop powder, and the addition amount of the cannabidiol is 0.1-1% of the mass of the dried hop powder.
4. The method for the modified preparation of hops according to claim 1, characterized in that: the pH regulator is one or more of sodium hydroxide, sodium carbonate, sodium bicarbonate, potassium hydroxide, potassium carbonate and potassium bicarbonate.
5. The method for the modified preparation of hops according to claim 1, characterized in that: the temperature of the multi-effect external circulation evaporator is 40-75 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210414280.5A CN114831305A (en) | 2022-04-20 | 2022-04-20 | Method for preparing hop by modification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210414280.5A CN114831305A (en) | 2022-04-20 | 2022-04-20 | Method for preparing hop by modification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114831305A true CN114831305A (en) | 2022-08-02 |
Family
ID=82566783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210414280.5A Pending CN114831305A (en) | 2022-04-20 | 2022-04-20 | Method for preparing hop by modification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114831305A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070254063A1 (en) * | 2006-04-07 | 2007-11-01 | Chemisch En Biochemisch Onderzoekscentrum (Cbok) | Use of hop polyphenols in beer |
| CN112680301A (en) * | 2021-01-19 | 2021-04-20 | 哈尔滨宝邦生物科技发展有限公司 | Beer making process with CBD |
| CN113234552A (en) * | 2021-04-29 | 2021-08-10 | 浙江大学 | Hop polysaccharide nano particle and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070254063A1 (en) * | 2006-04-07 | 2007-11-01 | Chemisch En Biochemisch Onderzoekscentrum (Cbok) | Use of hop polyphenols in beer |
| CN112680301A (en) * | 2021-01-19 | 2021-04-20 | 哈尔滨宝邦生物科技发展有限公司 | Beer making process with CBD |
| CN113234552A (en) * | 2021-04-29 | 2021-08-10 | 浙江大学 | Hop polysaccharide nano particle and preparation method and application thereof |
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