CN114813521B - Gain calibration method and system, and related device - Google Patents
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Abstract
The application discloses a gain calibration method, a system and a related device, wherein the gain calibration method specifically comprises the following steps: judging whether the first central value meets a first preset condition or not according to the first central value of the calibrator under the first gain and the original central value of the calibrator; if not, setting a second gain based on the first gain; judging whether the second central value meets a second preset condition or not according to the second central value of the calibrator under the second gain; if the gain curve does not meet the requirement, a gain curve is obtained by using the first central value and the second central value under two times of different gains; acquiring a target gain by using the original central value of the calibrator based on the gain curve; obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset check condition or not based on the third central value and the original central value; and if so, taking the target gain as the gain value of the current instrument. By the aid of the method, the debugging efficiency and accuracy of the instrument can be improved.
Description
Technical Field
The present application relates to the field of blood detection technologies, and in particular, to a gain calibration method and system, and a related apparatus.
Background
Flow analyzers have been widely used in clinical and laboratory fields as instruments for counting and classifying blood cell parameters of human bodies, and mainly provide test parameters such as Red Blood Cells (RBC), mean Corpuscular Volume (MCV), hematocrit (HCT), mean Platelet Volume (MPV), and reticulocyte hemoglobin content (RHE), and test results are used for diagnosing the state of illness of patients by clinicians, so that high requirements are placed on accuracy and reliability of the test results.
However, the inventors have found that, through long-term research, different hardware or installation errors affect the accuracy of the measurement results of different detection instruments, and therefore, before impedance or optical detection is used, an operation of calibrating the initial gain of the detector with a standard substance is required to reduce the deviation of the results between different instruments. However, the existing method for calibrating the gain between different instruments often depends on the experience of the user to perform one or more calibration. The above method of gain calibration depending on user experience results in low efficiency and accuracy of gain calibration, and further affects efficiency and accuracy of debugging between instruments, so the method is to be improved.
Disclosure of Invention
The technical problem mainly solved by the application is to provide a gain calibration method, a gain calibration system and a related device, which can reduce the difference of measurement results among different instruments and improve the debugging efficiency and accuracy of the instruments.
In order to solve the technical problem, the application adopts a technical scheme that: the method comprises the steps of obtaining a first central value of a calibration object under a first gain, and judging whether the first central value meets a first preset condition or not based on the first central value and an original central value of the calibration object; wherein, the first gain is a current first preset value of the instrument;
if the first central value does not meet the first preset condition, acquiring a second gain of the calibration object based on the first central value, the original central value and the first gain;
obtaining a second central value of the calibrator under the second gain, and determining whether the second central value meets a second preset condition or not based on the second central value and the original central value of the calibrator;
if the second central value does not meet the second preset condition, obtaining a gain curve by using the first central value and the second central value under two different gains;
acquiring a target gain by using the original central value of the calibrator based on the gain curve;
obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset check condition or not based on the third central value and the original central value;
and if the target gain meets the preset verification condition, taking the target gain as the gain value of the current instrument.
Wherein the method further comprises:
and if the first central value meets the first preset condition, taking the first gain as the gain value of the current instrument.
Wherein obtaining a second gain of the calibrant based on the first center value, the original center value, and the first gain comprises:
judging whether the first central value is smaller than the original central value;
if the first central value is smaller than the original central value, taking a sum of the first gain and a second preset value as a first candidate gain, obtaining a second candidate gain according to the first central value, the original central value and the first gain, and determining a maximum gain value from the first candidate gain and the second candidate gain as the second gain;
if the first central value is larger than the original central value, taking a difference value between the first gain and a third preset value as a third candidate gain, obtaining a fourth candidate gain according to the first central value, the original central value and the first gain, and determining a minimum gain value from the third candidate gain and the fourth candidate gain as the second gain.
Wherein the step of obtaining a gain curve using the first central value and the second central value at the two different gains comprises:
obtaining a slope and an intercept of the gain curve based on the first gain, the first center value, the second gain, and the second center value.
Wherein the step of determining whether the target gain satisfies a preset check condition based on the third center value and the original center value includes:
obtaining a difference value between the third central value and the original central value, and obtaining a ratio of the difference value to the original central value;
and judging whether the absolute value of the ratio is smaller than or equal to a fourth preset value or not, and determining whether the target gain is used as the gain value of the current instrument or not based on the judgment result.
After the step of determining whether the absolute value of the ratio is smaller than or equal to a fourth preset value and determining whether the target gain is used as the gain value of the current instrument based on the determination result, the method includes:
if the absolute value of the ratio is smaller than or equal to the fourth preset value, taking the target gain as the gain value of the current instrument;
and if the absolute value of the ratio is greater than the fourth preset value, sending alarm information.
Wherein the method further comprises:
and if the second central value meets the second preset condition, taking the second gain as the gain value of the current instrument.
In order to solve the technical problem, the other technical scheme adopted by the application is as follows: there is provided a gain calibration system comprising: a detector for detecting a signal generated by each particle to be measured in the calibrator; a booster that receives the signal obtained by the detector and amplifies the signal based on a gain set in the booster; a controller for measuring a first central value and a second central value of the calibrant at two different gains; obtaining a gain curve by using the first central value and the second central value under the two different gains; acquiring a target gain by using the original central value of the calibrator based on the gain curve; obtaining a third central value corresponding to the target gain, and judging whether the target gain meets a preset condition or not based on the third central value and the original central value; and if the target gain meets the preset condition, taking the target gain as the gain value of the current instrument.
Wherein the gain calibration system comprises any one of an optical gain calibration system and an impedance gain calibration system.
In order to solve the above technical problem, another technical solution adopted by the present application is: there is provided a gain calibration apparatus comprising a processor and a memory coupled to each other, the processor and the memory cooperating with each other to implement the gain calibration method mentioned in any of the above embodiments.
Different from the prior art, the beneficial effects of the application are that: the application provides a gain calibration method, which specifically comprises the following steps: judging whether the first central value meets a first preset condition or not according to the first central value of the calibrator under the first gain and the original central value of the calibrator; if not, setting a second gain based on the first gain; judging whether the second central value meets a second preset condition or not according to the second central value of the calibrator under the second gain; if not, obtaining a gain curve by using the first central value and the second central value under the two different gains; acquiring a target gain by using the original central value of the calibrator based on the gain curve; and obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset condition or not based on the third central value and the original central value. By the aid of the design scheme, whether the second gain needs to be acquired and whether the corresponding central value needs to be acquired are judged through the first central value corresponding to the first gain, whether subsequent calibration needs to be performed can be judged quickly, and gain calibration efficiency is effectively improved; similarly, whether the gain curve needs to be acquired or not is judged through a second central value corresponding to the second gain, whether subsequent calibration needs to be performed or not can be quickly judged, and the gain calibration efficiency is effectively improved; acquiring a central value under two gains, acquiring a gain curve of the instrument, and determining a gain value corresponding to a target value, on one hand, the method can effectively reduce the measurement result deviation among different instruments, and improve the consistency among different instruments; on the other hand, the method can simplify the debugging link in the production process, directly completes the gain debugging in the installation link, does not need to debug the gain value again after the instrument outputs the parameters, and further improves the efficiency of instrument debugging.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic flow chart diagram illustrating an embodiment of a gain calibration method of the present application;
FIG. 2 is a schematic flow chart diagram illustrating another embodiment of the gain calibration method of the present application;
FIG. 3 is a schematic flow chart of an embodiment before step S103;
FIG. 4 is a schematic flow chart of one embodiment of step S106 in FIG. 1;
FIG. 5 is a block diagram of an embodiment of the gain calibration system of the present application;
FIG. 6 is a schematic structural diagram of an embodiment of the gain calibration apparatus of the present application;
fig. 7 is a schematic diagram of a frame of an embodiment of a memory device with a gain calibration function according to the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
Referring to fig. 1, fig. 1 is a schematic flow chart illustrating an embodiment of a gain calibration method according to the present application. The application provides a gain calibration method, which specifically comprises the following steps:
s101: acquiring a first central value of a calibration object under a first gain, and judging whether the first central value meets a first preset condition or not based on the first central value and an original central value of the calibration object; and the first gain is a current first preset value of the instrument.
Specifically, the calibrator may be a standard particle, a calibrator, or a conventional blood sample. For the impedance system, the center values refer to the RBC red cell histogram center and the WBC white cell histogram center; for optical systems, the central value refers to the central value of the bottom, middle, high or front, side, or fluorescence for the target value of the standard. The ranges of the gain and the center value are not fixed values for different instrument chips, but vary according to chips of different manufacturers. In the present embodiment, the gain has a numerical value in the range of 0 to 255 and a central value in the range of 0 to 4095.
In this embodiment, if the first central value satisfies the first preset condition, the first gain is used as the gain value of the current instrument. After the gain calibration is carried out once, the first central value can meet the first preset condition, the current gain calibration is considered to be finished, the calibration for the second time or even more subsequent times is not needed, and the gain calibration efficiency of the instrument is effectively improved.
Determining whether the first central value satisfies the first preset condition in step S101 may further include: acquiring a difference value between the first central value and the original central value, and acquiring a ratio of the difference value to the original central value; and judging whether the absolute value of the ratio is smaller than or equal to the deviation allowable range of the current instrument.
S102: and if the first central value does not meet the first preset condition, acquiring a second gain of the calibration object based on the first central value, the original central value and the first gain, wherein the second gain is different from the first gain.
Referring to fig. 2, fig. 2 is a schematic flowchart illustrating a process of obtaining a center value according to different gains according to another embodiment of the gain calibration method of the present application, which includes the following steps:
s201: obtaining a first center value of the calibrator at a first gain; wherein, the first gain is a current first preset value of the instrument.
Specifically, the first gain is an original gain in the instrument, that is, a first preset value preset in the system by a manufacturer before the instrument leaves a factory or a first preset value set when the instrument is used last time. In the present embodiment, the first preset value is any one of values from 0 to 255. The first center value is a current center value of the particle mass obtained by measuring the calibrant with the instrument at the first gain.
S202: obtaining a second center value of the calibrator at a second gain; wherein the second gain is set according to the first gain.
Specifically, in the present embodiment, the second gain is obtained according to the first gain adjustment, and the specific adjustment process will be described in detail in the following examples. In the present embodiment, the value of the second gain may be any value from 0 to 255. The second center value is a current center value of the particle mass obtained by measuring the calibrant with the instrument at the second gain.
Through the embodiment, the first central value and the second central value are respectively obtained by utilizing the first gain and the second gain which are set successively, different central values under two different gains are obtained through collection, and technical support is provided for the subsequent process of obtaining the gain curve when the second gain does not meet the second preset condition.
Referring to fig. 3, fig. 3 is a schematic flow chart of an embodiment before step S103. In this embodiment, how to set the second gain according to the first gain is specifically described, the step S103 includes the following steps:
s301: and judging whether the first central value is smaller than the original central value.
S302: if the first central value is smaller than the original central value, taking a sum of the first gain and a second preset value as a first candidate gain, obtaining a second candidate gain according to the first central value, the original central value and the first gain, and determining a maximum gain value from the first candidate gain and the second candidate gain as the second gain.
Specifically, in the present embodiment, the size of the second preset value is manually set according to the requirement, for example, if the gain range of the current instrument is 0-255, the second preset value can be set to 20.
And if the first center value is smaller than the original center value, taking the sum of the first gain and a second preset value as a first candidate gain, calculating the product of the first gain and the original center according to the first gain and the original center, taking the quotient of the product and the first center value as a second candidate gain, and determining the maximum gain value from the first candidate gain and the second candidate gain as the second gain. Exemplarily, the second gain g2= max (g 1+20, (g 1 × m) 0 ）/ m 1 ) The first candidate gain is g1+20, and the second candidate gain is (g 1 × m) 0 ）/ m 1 (ii) a Where g1 is the first gain, m 0 Is the original center value, m 1 Max represents taking the maximum value as the first central value.
S303: if the first central value is larger than the original central value, taking a difference value between the first gain and a third preset value as a third candidate gain, obtaining a fourth candidate gain according to the first central value, the original central value and the first gain, and determining a minimum gain value from the third candidate gain and the fourth candidate gain as the second gain.
In this embodiment, the third preset value is manually set according to the requirement, for example, the gain range of the current instrument is 0-255, and then the second preset value can be set to 20.
Specifically, if the first center value is greater than the original center value, the difference between the first gain and a third preset value is used as a third candidate gain, the product of the first gain and the original center is calculated according to the first gain and the original center, and the product is usedA quotient value with the first center value is used as a fourth candidate gain, and a maximum gain value is determined from the third candidate gain and the fourth candidate gain as the second gain. Exemplarily, the second gain g2= min (g 1-20, (g 1 × m) 0 ）/ m 1 ) The third candidate gain is g1-20, the fourth candidate gain is (g 1 x m) 0 ）/ m 1 (ii) a Where g1 is the first gain, m 0 Is the original central value, m 1 Min represents taking the minimum value as the first central value.
Through the embodiment, the obtained first gain, the first central value, the second gain and the second central value can be ensured to fall in the effective value range of the gain curve, the problem of parameter overflow is prevented, and errors in gain setting are avoided. And a second gain is set through the deviation of the first central value and the original central value, so that the second gain closer to the target gain can be better determined, and the efficiency of gain calibration is effectively improved.
S103: and obtaining a second central value of the calibrator under the second gain, and judging whether the second central value meets a second preset condition or not based on the second central value and the original central value of the calibrator.
In this embodiment, if the second central value satisfies the second preset condition, the second gain is used as the gain value of the current instrument. After the gain calibration is performed twice, the second central value can meet the second preset condition, and then the current gain calibration is considered to be finished, so that third or even subsequent calibration for many times is not needed, and the gain calibration efficiency of the instrument is effectively improved.
Determining whether the second central value satisfies a second preset condition in step S103 may further include: acquiring a difference value between the second central value and the original central value, and acquiring a ratio of the difference value to the original central value; and judging whether the absolute value of the ratio is smaller than or equal to the deviation allowable range of the current instrument.
S104: and if the second central value does not meet the second preset condition, obtaining a gain curve by using the first central value and the second central value under two different gains.
In this embodiment, the step S104 specifically includes: based on the first gain g 1 First central value m 1 A second gain g 2 And a second central value m 2 Obtaining the slope a and the intercept b of a gain curve; wherein the first gain g 1 And a second gain g 2 The corresponding gain curve in the gain range of (2) is a linear gain curve. The gain curve includes at least a first gain g in consideration of the gain calibration accuracy 1 And a second gain g 2 Linear gain curve of (1). Specifically, using a first gain g 1 First central value m 1 Constructing a linear equation of two 1 =a*g 1 + b using a second gain g 2 And a second central value m 2 Construction of another equation of a two-dimensional equation m 2 =a*g 2 And + b, combining the two equations to form a linear equation system, and solving to obtain the slope a and the intercept b of the gain curve. Through the implementation mode, a linear gain curve is determined by using two different gains and central values, and the slope and intercept in the linear gain curve are obtained by using a linear equation system, so that a foundation is laid for determining a reasonable gain value for a subsequent calibrator.
S105: based on the gain curve, the target gain is obtained using the raw center value of the calibrant.
Specifically, the original center value of the calibrant is directly read by referring to a table of target values corresponding to calibrants each corresponding to a determined target value. In the present embodiment, the obtained gain curve is m = a × g + b, where g represents a gain value of the gain curve, a and b represent a slope and an intercept of the gain curve, respectively, and m represents a center value. Looking up to obtain the original center value m of the current calibration object 0 Then the target gain g 0 =（m 0 -b）/a。
S106: and obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset check condition or not based on the third central value and the original central value.
S107: and if the target gain meets the preset check condition, taking the target gain as the gain value of the current instrument. Specifically, the current gain of the instrument is modified to be the target gain, a third central value related to the calibration object is measured under the target gain, and whether the target gain meets the preset check condition is determined based on the third central value and the original central value, and specific descriptions related to the preset check condition will be described in detail in the following embodiments.
Referring to fig. 4, fig. 4 is a flowchart illustrating an embodiment of step S106 in fig. 1. The present embodiment introduces the preset verification condition mentioned in step S106 in detail, where step S106 specifically includes the following steps:
s401: and acquiring a difference value between the third central value and the original central value, and acquiring a ratio of the difference value to the original central value.
S402: and judging whether the absolute value of the ratio is smaller than or equal to a fourth preset value or not, and determining whether the target gain is used as the gain value of the current instrument or not based on the judgment result.
Specifically, the fourth preset value is an allowable deviation range between the third central value and the original central value, and the size of the fourth preset value can be set by itself, and generally, the size of the fourth preset value is preferably 5%. In the present embodiment, first, the third central value m is acquired c And the original center value m 0 The difference between, i.e. m c - m 0 Then, the ratio (m) between the difference and the original central value is obtained c - m 0 )/ m 0 The absolute value abs of the ratio is determined ((m) c - m 0 )/ m 0 ) And whether the deviation is smaller than or equal to a fourth preset value Ra, wherein Ra is the deviation allowable range of the current instrument.
Through the implementation mode, the effect of gain calibration is verified by using the third central value and the original central value, whether the obtained target gain meets the requirement or not is judged, and technical support is provided for the gain calibration process.
Referring to fig. 4, after the step S402, the method further includes:
s403: and if the absolute value of the ratio is smaller than or equal to the fourth preset value, taking the target gain as the gain value of the current instrument.
Specifically, in this embodiment, if the absolute value of the ratio is within the deviation allowable range corresponding to the fourth preset value, it indicates that the calibration result meets the requirement, and the target gain is set as the gain value of the current instrument.
S404: and if the absolute value of the ratio is greater than the fourth preset value, sending alarm information and carrying out manual inspection.
Specifically, if the absolute value of the ratio exceeds the deviation allowable range, an alarm is given, and an engineer is reminded to check the abnormal condition of the instrument. By the embodiment, whether the second gain needs to be acquired and the corresponding central value needs to be acquired is judged through the first central value corresponding to the first gain, whether subsequent calibration needs to be performed can be quickly judged, and the gain calibration efficiency is effectively improved; similarly, whether the gain curve needs to be acquired or not is judged through a second central value corresponding to the second gain, whether subsequent calibration needs to be performed or not can be quickly judged, and the gain calibration efficiency is effectively improved; acquiring a central value under two gains, acquiring a gain curve of the instrument, and determining a gain value corresponding to a target value, on one hand, the method can effectively reduce the measurement result deviation among different instruments, and improve the consistency among different instruments; on the other hand, the method can simplify the debugging link in the production process, directly completes the gain debugging in the installation link, does not need to debug the gain value again after the instrument outputs the parameters, and further improves the efficiency of instrument debugging. Through the implementation mode, the debugging among different instruments is realized by utilizing a clear and uniform gain calibration method instead of depending on the experience of a user to carry out gain calibration, and the efficiency and the accuracy of gain calibration are effectively improved.
In an embodiment, after step S403, the method further includes: and exiting the calibration interface in response to the target gain being used as the gain value of the current instrument. In addition, alarm information can show on the calibration interface, or alarm information accessible other modes send out in order to play the warning effect, if: the sound alarm, the photoelectric alarm, etc. are not limited in particular.
Referring to fig. 5, fig. 5 is a block diagram of an embodiment of a gain calibration system according to the present application. The present application further provides a gain calibration system 100, which specifically includes a detector 10, a booster 20, and a controller 30. The detector 10 is used for detecting a signal generated by each particle to be detected in a calibrator, which may be selected from a standard particle, a calibrator, or a conventional blood sample. The booster 20 receives the signal obtained by the detector and amplifies the signal based on the gain set in the booster 20. The controller 30 is configured to complete a gain calibration process, which specifically includes measuring a first central value and a second central value of the calibrator under two different gains; obtaining a gain curve by using the first central value and the second central value under two times of different gains; based on the gain curve, acquiring a target gain by using the original central value of the calibrator; obtaining a third central value corresponding to the target gain, and judging whether the target gain meets a preset check condition or not based on the third central value and the original central value; and if the target gain meets the preset verification condition, taking the target gain as the gain value of the current instrument. Through the above embodiment, the gain calibration process is realized by using the gain calibration system, the central value under two gains is collected by the controller 30, the gain curve of the instrument is obtained, and the gain value corresponding to the target value is determined, on one hand, the method can effectively reduce the measurement result deviation between different instruments, and improve the consistency between different instruments; on the other hand, the method can simplify the debugging link in the production process, directly completes the gain debugging in the impact link, does not need to debug the gain value again after the instrument outputs parameters, and further improves the efficiency of instrument debugging.
With continued reference to fig. 5, the gain calibration system provided herein further includes a particle director 40 and a driving element 50. Wherein the particle guide 40 is used for loading and orderly releasing each particle into the detector 10; the drive assembly 50 may include a plurality of mechanical drives including motors, valves, pumps, etc. for loading ions in the calibrant into the particle guide 40.
Referring again to fig. 5, in another embodiment, the gain calibration system provided by the present application further includes a signal source 60 and a memory 70. Wherein the signal source 60 is used to excite and release the signal and the memory 70 is used to store the latest set effective gain value in the system. The processing of the calibration material by the gain calibration system 100 provided in the present application is specifically as follows: the signal excited by the signal source 60 changes when the particle passes through, and the changed signal is captured by the detector 10, amplified by the gain unit 20, and transmitted to the controller 30 to calculate the center position of the particle.
In a specific application scenario, the gain calibration system provided by the present application includes an optical gain calibration system. Specifically, in the optical gain calibration system, a laser is selected as a signal source, a flow type sheath flow device is selected as a particle guide device, and a light sensing device is selected as a detector. The optical calibration system mentioned in the above embodiments can be used for calibration of optical signals at different angles in the optical gain calibration system, for example, a base angle signal (1-5 °), a medium angle signal (5-10 °), and a high angle signal (10-90 °). In addition, a fluorescence channel is added in the optical gain calibration system, a fluorescence staining technology is matched, an optical filter is added in the detector, and the method for calibrating the fluorescence signal is also applicable. In the embodiment, the optical gain calibration system is used for acquiring optical signals at different angles, and the gain calibration can be realized for the signals at different angles.
In a further specific application scenario, the gain calibration system provided by the present application includes an impedance gain calibration system. Specifically, in the impedance calibration system, a constant current system is selected as a signal source, a gem hole of the impedance device is selected as a particle guide, and an analog-to-digital conversion device is selected as a detector. Through the above embodiment, the signal acquired by the impedance device is gain-calibrated by using the impedance gain calibration system.
Referring to fig. 6, fig. 6 is a schematic structural diagram of an embodiment of a gain calibration apparatus according to the present application. The apparatus 200 includes a memory 201 and a processor 202 coupled to each other, the memory 201 stores program instructions, and the processor 202 is configured to execute the program instructions to implement the gain calibration method mentioned in any of the above embodiments.
Specifically, the processor 202 may also be referred to as a CPU (Central Processing Unit). The processor 202 may be an integrated circuit chip having signal processing capabilities. The Processor 202 may also be a general purpose Processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA) or other Programmable logic device, discrete Gate or transistor logic, discrete hardware components. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. In addition, processor 202 may be implemented collectively by a plurality of integrated circuit chips.
Referring to fig. 7, fig. 7 is a block diagram illustrating a memory device with gain calibration according to an embodiment of the present invention. The memory means 300 has program data 301 that can be read by a computer, the program data 301 also being executable by a processor, the program data 301 being adapted to implement the gain calibration method as mentioned in any of the embodiments above. The program data 301 may be stored in the above apparatus with a storage function in the form of a software product, and includes several instructions to enable a computer device (which may be a personal computer, a server, or a network device) or a processor (processor) to execute all or part of the steps of the method according to the embodiments of the present application. The foregoing storage device includes: various media capable of storing program codes, such as a usb disk, a mobile hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, or terminal devices, such as a computer, a server, a mobile phone, and a tablet.
In summary, unlike the prior art, the present application provides a gain calibration method, which specifically includes: judging whether the first central value meets a first preset condition or not according to the first central value of the calibrator under the first gain and the original central value of the calibrator; if not, setting a second gain based on the first gain; judging whether the second central value meets a second preset condition or not according to the second central value of the calibrator under the second gain; if not, obtaining a gain curve by using the first central value and the second central value under the two different gains; acquiring a target gain by using the original central value of the calibrator based on the gain curve; and obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset condition or not based on the third central value and the original central value. By the aid of the design scheme, whether the second gain needs to be acquired and whether the corresponding central value needs to be acquired are judged through the first central value corresponding to the first gain, whether subsequent calibration needs to be performed can be judged quickly, and gain calibration efficiency is effectively improved; similarly, whether the gain curve needs to be acquired or not is judged through a second central value corresponding to the second gain, whether subsequent calibration needs to be performed or not can be quickly judged, and the gain calibration efficiency is effectively improved; on one hand, the method can effectively reduce the measurement result deviation among different instruments and improve the consistency among different instruments; for example, the optical five classification percentages, reticulin content or MCV (mean corpuscular volume), MPV (platelet mean volume) of impedance measured by different instruments are made closer to the relevant parameters of the same volume; for example, the average deviation of some parameters of different instruments before gain adjustment is as follows: MCV (mean corpuscular volume) 5.49%, MPV (mean corpuscular volume) 7.43%, RHE (reticuloendothelial hemoglobin content) 5.98%; the average deviation of partial parameters after different instruments gain-debug in this way is as follows: MCV (mean corpuscular volume) 0.36%, MPV (mean corpuscular volume) 0.43%, RHE (reticuloendothelial hemoglobin content) 0.01%; on the other hand, the method can simplify the debugging link in the production process, directly completes the gain debugging in the installation link, does not need to debug the gain value again after the instrument outputs the parameters, and further improves the efficiency of instrument debugging.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (8)
1. A method of gain calibration, comprising:
acquiring a first central value of a calibration object under a first gain, and judging whether the first central value meets a first preset condition or not based on the first central value and an original central value of the calibration object;
if the first central value does not meet the first preset condition, acquiring a second gain of the calibration object based on the first central value, the original central value and the first gain, wherein the second gain is different from the first gain;
obtaining a second central value of the calibrator under the second gain, and determining whether the second central value meets a second preset condition or not based on the second central value and the original central value of the calibrator;
if the second central value does not meet the second preset condition, obtaining a gain curve by using the first central value and the second central value under different gains twice;
acquiring a target gain by using the original central value of the calibrator based on the gain curve;
obtaining a third central value of the calibration object under the target gain, and judging whether the target gain meets a preset check condition or not based on the third central value and the original central value;
if the target gain meets the preset verification condition, taking the target gain as a gain value of the current instrument;
wherein obtaining a second gain of the calibrant based on the first center value, the original center value, and the first gain comprises: judging whether the first central value is smaller than the original central value; if the first central value is smaller than the original central value, taking a sum of the first gain and a second preset value as a first candidate gain, obtaining a second candidate gain according to the first central value, the original central value and the first gain, and determining a maximum gain value from the first candidate gain and the second candidate gain as the second gain; if the first central value is larger than the original central value, taking a difference value between the first gain and a third preset value as a third candidate gain, acquiring a fourth candidate gain according to the first central value, the original central value and the first gain, and determining a minimum gain value from the third candidate gain and the fourth candidate gain as the second gain;
wherein the step of obtaining a gain curve using the first central value and the second central value at the two different gains comprises: obtaining a slope and an intercept of the gain curve based on the first gain, the first center value, the second gain, and the second center value.
2. The gain calibration method of claim 1, further comprising:
and if the first central value meets the first preset condition, taking the first gain as the gain value of the current instrument.
3. The method according to claim 1, wherein the step of determining whether the target gain satisfies a predetermined check condition based on the third center value and the original center value comprises:
obtaining a difference value between the third central value and the original central value, and obtaining a ratio of the difference value to the original central value;
and judging whether the absolute value of the ratio is smaller than or equal to a fourth preset value or not, and determining whether the target gain is used as the gain value of the current instrument or not based on the judgment result.
4. The gain calibration method according to claim 3, wherein the step of determining whether the absolute value of the ratio is less than or equal to a fourth preset value and determining whether to use the target gain as the gain value of the current instrument based on the determination result comprises:
if the absolute value of the ratio is smaller than or equal to the fourth preset value, taking the target gain as the gain value of the current instrument;
and if the absolute value of the ratio is greater than the fourth preset value, sending alarm information.
5. The gain calibration method of claim 1, further comprising:
and if the second central value meets the second preset condition, taking the second gain as the gain value of the current instrument.
6. A gain calibration system, comprising:
a detector for detecting a signal generated by each particle to be measured in the calibrator;
a booster that receives the signal obtained by the detector and amplifies the signal based on a gain set in the booster;
a controller for measuring a first central value and a second central value of the calibrant at two different gains; obtaining a gain curve by using the first central value and the second central value under the two different gains; acquiring a target gain by using the original central value of the calibrator based on the gain curve; obtaining a third central value corresponding to the target gain, and judging whether the target gain meets a preset condition or not based on the third central value and the original central value; if the target gain meets the preset condition, taking the target gain as a gain value of the current instrument;
wherein said measuring a first center value and a second center value of said calibrator at two different gains comprises: acquiring a first central value of the calibrator under the first gain; if the first central value does not meet a first preset condition, acquiring a second gain of the calibration object based on the first central value, the original central value and the first gain, and acquiring a second central value of the calibration object under the second gain;
wherein obtaining a gain curve using the first central value and the second central value at the two different gains comprises: if the second central value does not meet a second preset condition, obtaining a gain curve by using the first central value and the second central value under different gains twice; wherein the obtaining a gain curve using the first central value and the second central value at two different gains comprises: obtaining a slope and an intercept of the gain curve based on the first gain, the first center value, the second gain, and the second center value.
7. The gain calibration system of claim 6,
the gain calibration system includes any one of an optical gain calibration system and an impedance gain calibration system.
8. A gain calibration apparatus comprising a processor and a memory coupled to each other, the processor and the memory cooperating with each other to implement the gain calibration method of any one of claims 1 to 5.
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