CN109239016B - Blood sample detection method and device and readable storage medium - Google Patents
Blood sample detection method and device and readable storage medium Download PDFInfo
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Abstract
The invention discloses a blood sample detection method, which comprises the following steps: acquiring a first threshold value; obtaining a first scattered photoelectric value of a blood sample; comparing a first scattered photoelectric value of the blood sample to a first threshold value; when the first scattered photoelectric value of the blood sample is larger than the first threshold value, judging that the blood sample is a full blood sample with sufficient liquid quantity; and when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, judging that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid quantity. The invention also discloses a blood sample detection device and a readable storage medium. The invention can accurately detect and judge whether the blood sample is a whole blood sample or not and whether the liquid amount of the whole blood sample is sufficient or not, and solves the problems of insufficient sampling of the whole blood sample and artificial selection or wrong placement of the sample type.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis medical instruments, in particular to a blood sample detection method, a blood sample detection device and a readable storage medium.
Background
According to the traditional method for controlling the liquid amount of the whole blood sample, the liquid level of the whole blood sample is judged through resistance type liquid level detection, capacitance type liquid level detection or pressure type liquid level detection, and then the liquid amount is ensured to be absorbed through the known shape of a fixed container and the set liquid level detection depth. When the liquid level of the sample is judged, the liquid level control method is easily interfered to influence the judgment result, so that the whole blood sample is possibly insufficient to be sucked, and the accuracy of the test result is further influenced.
According to the traditional instrument operation method, the type of a blood sample to be detected, such as a whole blood sample, a serum sample, a plasma sample and the like, is input by manual operation, and if the whole blood sample is detected, the type of a non-whole blood sample is input manually or placed, so that the type of the sample set by the instrument is not matched with the type of the whole blood sample actually detected, a difference in result is caused, and even a complete error in result is caused.
Therefore, how to avoid the situation that the liquid volume of the whole blood sample is not sufficiently sucked due to the air suction generated when the conventional liquid level detection is subjected to false detection and how to automatically avoid the situation that the whole blood sample type is selected incorrectly or a non-whole blood sample is placed due to manual misoperation by an instrument is a technical problem which needs to be solved urgently by a person skilled in the art.
The traditional turbidimetry is applied to collecting scattered photoelectric values of a sample, an antibody and other related reagent mixed solutions, and the concentration of the sample to be detected is judged by a terminal point method, a kinetic method and the like. And there is no embodiment in which turbidimetry is used to detect the type of blood sample and whether the amount of sample liquid is sufficient.
Disclosure of Invention
The invention mainly aims to provide a method and a device for detecting a blood sample and a readable storage medium, aiming at accurately detecting and judging whether the blood sample is a whole blood sample or not and whether the liquid volume of the whole blood sample is sufficient or not, and solving the problems of insufficient sampling of the whole blood sample and artificial selection or wrong placement of the sample type.
In order to achieve the above object, the present invention provides a blood sample detection method, comprising the steps of:
acquiring a first threshold value;
obtaining a first scattered photoelectric value of a blood sample;
comparing a first scattered photoelectric value of the blood sample to a first threshold value;
when the first scattered photoelectric value of the blood sample is larger than the first threshold value, judging that the blood sample is a full blood sample with sufficient liquid quantity;
and when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, judging that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid quantity.
Preferably, the step of obtaining the first threshold value includes:
detecting the non-whole blood test samples with different liquid amounts and the whole blood test samples with different liquid amounts by adopting a turbidimetric analysis unit to obtain a second scattered photoelectric value of the non-whole blood test samples with different liquid amounts and a third scattered photoelectric value of the whole blood test samples with different liquid amounts;
and performing statistical analysis according to the second scattered photoelectric value and the third scattered photoelectric value to obtain a first threshold value.
Preferably, the step of obtaining a first scattered photoelectric value of the blood sample comprises:
controlling a light source to emit a light beam to irradiate the blood sample in the detection channel so as to enable the light beam to be scattered; and receiving scattered light corresponding to the light beam through a photosensitive element, and converting the scattered light into a first scattered photoelectric value.
Preferably, the non-whole blood sample comprises: serum samples, plasma samples, buffer or buffer-mixed blood samples.
Preferably, after the step of determining that the blood sample is a full blood sample with a sufficient amount of liquid when the first scattered photoelectric value of the blood sample is greater than the first threshold value, the method further includes:
and when the blood sample is judged to be a whole blood sample with sufficient liquid quantity, outputting prompt information.
Preferably, when the blood sample is judged to be a whole blood sample with a sufficient amount of liquid, after the step of outputting the prompt information, the method further comprises:
the blood sample is analyzed by the test unit test.
Preferably, after the step of determining that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid volume when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, the method further includes:
when the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid quantity, comparing a first scattered photoelectric value of the blood sample with a preset second threshold value;
and when the first scattered photoelectric value of the blood sample is smaller than the second threshold value, judging that the blood sample is a non-whole blood sample.
Preferably, when the first scattered photoelectric value of the blood sample is greater than the second threshold, the step of determining that the blood sample is a whole blood sample with insufficient liquid volume, and when the first scattered photoelectric value of the blood sample is less than the second threshold, the step of determining that the blood sample is a non-whole blood sample further includes:
and when the blood sample is judged to be a whole blood sample with insufficient liquid volume, continuously sucking the liquid volume, and executing the step of obtaining the first scattered photoelectric value of the blood sample when the sucking is finished.
In order to achieve the above object, the present invention also provides a blood sample test device including: a turbidimetric analysis unit and a test unit, the blood sample testing device further comprising: a memory, a processor and a blood sample testing program stored on the memory and executable on the processor, the blood sample testing program when executed by the processor implementing the steps of the blood sample testing method as described above.
In addition, to achieve the above object, the present invention further provides a readable storage medium having stored thereon a blood sample detection program, which when executed by a processor, implements the steps of the blood sample detection method as described above.
The method includes acquiring a first threshold; obtaining a first scattered photoelectric value of a blood sample; comparing a first scattered photoelectric value of the blood sample to a first threshold value; when the first scattered photoelectric value of the blood sample is larger than the first threshold value, judging that the blood sample is a full blood sample with sufficient liquid quantity; and when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, judging that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid quantity. By the mode, the device can accurately detect and judge whether the blood sample is the whole blood sample or not and whether the liquid amount of the whole blood sample is sufficient or not, and solves the problem of insufficient sampling of the whole blood sample.
Drawings
Fig. 1 is a schematic terminal structure diagram of a hardware operating environment according to an embodiment of the present invention;
FIG. 2 is a schematic flow chart of a blood sample detection method according to a first embodiment of the present invention in FIG. 2;
FIG. 3 is a schematic flow chart of a second embodiment of the method for testing a blood sample according to the present invention;
FIG. 4 is a schematic flow chart of a blood sample detection method according to a third embodiment of the present invention;
FIG. 5 is a schematic flow chart of a blood sample testing method according to a fourth embodiment of the present invention;
FIG. 6 is a schematic flow chart of a fifth embodiment of the method for testing a blood sample according to the present invention;
FIG. 7 is a schematic flow chart of a blood sample testing method according to a sixth embodiment of the present invention;
FIG. 8 is a schematic flow chart of a blood sample testing method according to a seventh embodiment of the present invention.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The main solution of the embodiment of the invention is as follows:
the liquid level detection of the liquid level of the existing whole blood sample is easily interfered, false detection occurs, the liquid level can not be accurately sucked, and when the whole blood sample is detected, the type of the non-whole blood sample is manually input or the non-whole blood sample is placed, so that the sample type set by the instrument is not matched with the type of the whole blood sample actually detected, and the test result of the blood sample is influenced.
The blood sample detection method of the invention obtains a first scattered photoelectric value of a blood sample; comparing the first scattered photoelectric value with a preset first threshold value; when the first scattered photoelectric value of the blood sample is larger than the first threshold value, the blood sample is judged to be a whole blood sample with sufficient liquid amount, when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid amount, whether the blood sample is the whole blood sample with sufficient blood amount or not is accurately judged by detecting the first scattered photoelectric value of the blood sample and comparing the first scattered photoelectric value with the first threshold value, the liquid amount is ensured to be absorbed sufficiently, and the problem of artificial selection or placing error of the sample type is solved by output prompt of judgment information.
As shown in fig. 1, fig. 1 is a schematic terminal structure diagram of a hardware operating environment according to an embodiment of the present invention.
The terminal of the embodiment of the invention can be a PC, and can also be a mobile terminal device with a display function, such as a smart phone, a tablet computer, an MP4(Moving Picture Experts Group Audio Layer IV, Moving Picture Experts compression Standard Audio Layer 3) player, a portable computer, and the like.
As shown in fig. 1, the terminal may include: a processor 1001, such as a CPU, a network interface 1004, a user interface 1003, a memory 1005, a communication bus 1002. Wherein a communication bus 1002 is used to enable connective communication between these components. The user interface 1003 may include a Display screen (Display), an input unit such as a Keyboard (Keyboard), and the optional user interface 1003 may also include a standard wired interface, a wireless interface. The network interface 1004 may optionally include a standard wired interface, a wireless interface (e.g., WI-FI interface). The memory 1005 may be a high-speed RAM memory or a non-volatile memory (e.g., a magnetic disk memory). The memory 1005 may alternatively be a storage device separate from the processor 1001.
Preferably, the terminal may further include a camera, a Radio Frequency (RF) circuit, a sensor, an audio circuit, a WiFi module, and the like. Such as light sensors, motion sensors, and other sensors. Specifically, the light sensor may include an ambient light sensor that may adjust the brightness of the display screen according to the brightness of ambient light, and a proximity sensor that may turn off the display screen and/or the backlight when the mobile terminal is moved to the ear. As one of the motion sensors, the gravity acceleration sensor can detect the magnitude of acceleration in each direction (generally, three axes), detect the magnitude and direction of gravity when the mobile terminal is stationary, and can be used for applications (such as horizontal and vertical screen switching, related games, magnetometer attitude calibration), vibration recognition related functions (such as pedometer and tapping) and the like for recognizing the attitude of the mobile terminal; of course, the mobile terminal may also be configured with other sensors such as a gyroscope, a barometer, a hygrometer, a thermometer, and an infrared sensor, which are not described herein again.
Those skilled in the art will appreciate that the terminal structure shown in fig. 1 is not intended to be limiting and may include more or fewer components than those shown, or some components may be combined, or a different arrangement of components.
As shown in fig. 1, a memory 1005, which is one type of computer storage medium, may include an operating system, a network communication module, a user interface module, and a blood sample testing program.
In the terminal shown in fig. 1, the network interface 1004 is mainly used for connecting to a backend server and performing data communication with the backend server; the user interface 1003 is mainly used for connecting a client (user side) and performing data communication with the client; and the processor 1001 may be configured to invoke a blood sample testing procedure stored in the memory 1005 and perform the following operations:
acquiring a first threshold value;
obtaining a first scattered photoelectric value of a blood sample;
comparing a first scattered photoelectric value of the blood sample to a first threshold value;
when the first scattered photoelectric value of the blood sample is larger than the first threshold value, judging that the blood sample is a full blood sample with sufficient liquid quantity;
and when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, judging that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid quantity.
Further, the step of obtaining the first threshold includes:
detecting the non-whole blood test samples with different liquid amounts and the whole blood test samples with different liquid amounts by adopting a turbidimetric analysis unit to obtain a second scattered photoelectric value of the non-whole blood test samples with different liquid amounts and a third scattered photoelectric value of the whole blood test samples with different liquid amounts;
and performing statistical analysis according to the second scattered photoelectric value and the third scattered photoelectric value to obtain a first threshold value.
Further, the step of obtaining a first scattered photoelectric value of the blood sample comprises:
controlling a light source to emit a light beam to irradiate the blood sample in the detection channel so as to enable the light beam to be scattered; and receiving scattered light corresponding to the light beam through a photosensitive element, and converting the scattered light into a first scattered photoelectric value.
Further, the non-whole blood sample comprises: serum samples, plasma samples, buffer or buffer-mixed blood samples.
Further, after the step of determining that the blood sample is a full blood sample with a sufficient amount of liquid when the first scattered photoelectric value of the blood sample is greater than the first threshold value, the method further includes:
and when the blood sample is judged to be a whole blood sample with sufficient liquid quantity, outputting prompt information.
Further, when the blood sample is judged to be a whole blood sample with a sufficient amount of liquid, after the step of outputting the prompt information, the method further includes:
the blood sample is analyzed by the test unit test.
Further, after the step of determining that the blood sample is a non-whole blood sample or a whole blood sample with insufficient fluid volume when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, the method further includes:
when the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid quantity, comparing a first scattered photoelectric value of the blood sample with a preset second threshold value;
and when the first scattered photoelectric value of the blood sample is smaller than the second threshold value, judging that the blood sample is a non-whole blood sample.
Further, after the step of determining that the blood sample is a whole blood sample with insufficient fluid volume when the first scattered photoelectric value of the blood sample is greater than the second threshold value, the method further includes:
and when the blood sample is judged to be a whole blood sample with insufficient liquid volume, continuously sucking the liquid volume, and executing the step of obtaining the first scattered photoelectric value of the blood sample when the sucking is finished.
Based on the above hardware structure, the embodiment of the method of the present invention is provided.
Referring to fig. 2, fig. 2 is a schematic flow chart of a first embodiment of the blood sample detection method of the present invention, which includes:
step S10, acquiring a first threshold;
the sample type of the blood sample can be a whole blood sample type or a non-whole blood sample type, a scattered photoelectric value of the test sample is detected by a turbidimetric method, the turbidimetric method comprises a scattered turbidimetric method or a transmission turbidimetric method, and after the scattered photoelectric values of different liquid amounts and different types of blood test samples in a reaction cup are detected, a first threshold value for judging the whole blood sample with sufficient liquid amount is obtained.
Step S20, obtaining a first scattered photoelectric value of the blood sample;
and acquiring a first scattered photoelectric value of the blood sample in the reaction cup by turbidimetric detection.
Step S30, comparing the first scattered photoelectric value of the blood sample with a first threshold value;
the first threshold is set after statistical analysis by testing the scattered photoelectric value of the blood test sample, and is used for judging whether the blood sample is a whole blood sample with sufficient liquid quantity.
Step S40, when the first scattered photoelectric value of the blood sample is greater than the first threshold, determining that the blood sample is a sufficient amount of whole blood sample;
the first scattered photoelectric value of blood sample is greater than when the first threshold value, accurate judgement blood sample is the sufficient whole blood sample of liquid measure, and through the contrast of scattered photoelectric value, whether can accurately judge blood sample whole blood sample and whole blood sample liquid measure sufficient, solve the not enough problem of whole blood sample, can not further test analysis to blood sample and appear the result unusual, influence clinical diagnosis.
Step S50, when the first scattered photoelectric value of the blood sample is smaller than the first threshold, determining that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid volume.
When the first scattered photoelectric value is smaller than the first threshold value, the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid, and the whole blood sample with sufficient liquid, the whole blood sample with insufficient liquid and the non-whole blood sample are effectively distinguished through comparison with the first threshold value.
Further, referring to fig. 3, fig. 3 is a schematic flow chart of a blood sample detection method according to a second embodiment of the present invention. Based on the above embodiment, the step S10 includes:
step S11, detecting the non-whole blood test samples with different liquid amounts and the whole blood test samples with different liquid amounts by adopting a turbidimetric analysis unit to obtain a second scattered photoelectric value of the non-whole blood test samples with different liquid amounts and a third scattered photoelectric value of the whole blood test samples with different liquid amounts;
the turbidimetry unit comprises a scattering turbidimetry unit or a transmission turbidimetry unit, non-whole blood test samples with different liquid quantities and whole blood test samples with different liquid quantities are placed in the reaction cup, different test samples in the reaction cup are detected through the turbidimetry unit, and second scattered photoelectric values of the non-whole blood test samples with different liquid quantities and third scattered photoelectric values of the whole blood test samples with different liquid quantities are obtained.
Step S12, performing statistical analysis according to the second scattered photoelectric value and the third scattered photoelectric value to obtain a first threshold;
and performing data statistics analysis according to the first scattered photoelectric value and the second scattered photoelectric value of the test samples, obtaining the limit of the scattered photoelectric values of the whole blood test sample with sufficient liquid quantity, the non-whole blood test sample and the whole blood test sample with insufficient liquid quantity from the test, and setting the limit as a first threshold value for judging whether the whole blood sample with sufficient liquid quantity exists.
Further, referring to fig. 4, fig. 4 is a schematic flow chart of a blood sample detection method according to a third embodiment of the present invention. Based on the above embodiment, the step S20 includes:
step S21, controlling the light source to emit light beam to irradiate the blood sample in the detection channel so as to scatter the light beam;
the blood sample is placed in the reaction cup and placed in the detection channel of the turbidimetric analysis unit, the light beam emitted by the light source of the turbidimetric analysis unit irradiates the blood sample, and when the light beam passes through the blood sample in the reaction cup, the light beam is scattered due to the existence of particulate matters in the sample.
And step S22, receiving the scattered light corresponding to the light beam through the photosensitive element, and converting the scattered light into a first scattered photoelectric value.
A photosensitive element of the turbidimetric analysis unit is arranged at a certain included angle with an incident light path, receives corresponding scattered light after light beams pass through a blood sample through the photosensitive element, and converts the scattered light into a first scattered photoelectric value.
Further, the non-whole blood sample comprises: serum samples, plasma samples, buffer or buffer-mixed blood samples.
The blood sample is generally divided into a whole blood sample, a serum sample and a plasma sample, and in the blood detection, a mixed solution of a buffer solution, deionized water and the like may be added into the blood for detection, and if the samples are mixed together, the samples are difficult to distinguish, that is, the non-whole blood sample includes the blood sample of the serum sample, the plasma sample, the buffer solution and the mixed buffer solution.
Further, referring to fig. 5, fig. 5 is a schematic flow chart of a blood sample detection method according to a fourth embodiment of the present invention. Based on the embodiment shown in fig. 2, after step S40, the method further includes:
and step S60, inputting prompting information when the blood sample is judged to be a whole blood sample with sufficient liquid volume.
When the blood sample is judged to be the whole blood sample with sufficient liquid amount, the prompt information of the blood sample type is input, the blood sample type does not need to be input manually, the problem that the wrong blood sample type is input manually, so that the test result has errors or mistakes, and the clinical diagnosis is influenced is solved.
Further, referring to fig. 6, fig. 6 is a schematic flow chart of a fifth embodiment of the blood sample detection method of the present invention. Based on the above embodiment, after step S60, the method may further include:
step S70, analyzing the blood sample by the test unit test.
When the blood sample is judged to be a whole blood sample with sufficient blood volume, the requirement of the test solution amount and the requirement of the sample are met, the prompt information of the blood sample type is input, the blood sample type does not need to be input manually, the method is more intelligent, the errors caused manually are reduced, after the prompt is carried out, the test device carries out the test analysis of the blood sample, the tested blood sample is ensured to be consistent with the input blood sample type, the test result cannot be mistaken or wrong, and the clinical diagnosis is not influenced.
Further, referring to fig. 7, fig. 7 is a schematic flow chart of a blood sample detection method according to a sixth embodiment of the present invention. Based on the above embodiment, after step S50, the method further includes:
step S80, when the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid quantity, comparing the first scattered photoelectric value of the blood sample with a preset second threshold value;
the method comprises the steps of placing non-whole blood test samples with different liquid amounts and whole blood test samples with different liquid amounts in a reaction cup, detecting different test samples in the reaction cup through a turbidimetric analysis unit to obtain scattered photoelectric values of the test samples, carrying out data statistical analysis according to the scattered photoelectric values of the test samples, obtaining a limit of the scattered photoelectric values of the whole blood test samples with insufficient liquid amounts and the non-whole blood test samples from the test, and setting the limit as a second threshold for judging whether the whole blood samples with sufficient liquid amounts exist.
Step S90, when the first scattered photoelectric value of the blood sample is greater than the second threshold, determining that the blood sample is a whole blood sample with insufficient liquid volume, and when the first scattered photoelectric value of the blood sample is less than the second threshold, determining that the blood sample is a non-whole blood sample.
Whether the blood sample is the whole blood sample with insufficient liquid volume is judged through the second threshold value, if the whole blood sample with insufficient liquid volume, the liquid suction work can be continuously carried out, the whole blood sample with insufficient liquid volume is prevented from being tested and analyzed, the result is abnormal, and the clinical judgment is influenced.
Further, referring to fig. 8, fig. 8 is a schematic flow chart of a blood sample detection method according to a seventh embodiment of the present invention. Based on the above embodiment, after step S90, the method further includes:
step S100, when the blood sample is judged to be a whole blood sample with insufficient liquid volume, the liquid volume is continuously sucked, and when the sucking is finished, the step S20 is executed.
When the blood sample is judged to be a whole blood sample with insufficient liquid volume, the liquid volume is continuously sucked, after the sucking is completed, the step of obtaining the first scattered photoelectric value of the blood sample is executed, whether the liquid volume of the whole blood sample is sufficient or not is continuously detected, and the liquid suction is stopped until the blood sample is judged to be the whole blood sample with sufficient liquid volume, so that the further test and analysis work is carried out.
The invention also provides a blood sample detection device.
The blood sample detection device comprises a turbidimetric analysis unit and a test unit, and further comprises: a memory, a processor and a blood sample testing program stored on the memory and executable on the processor, the blood sample testing program when executed by the processor implementing the steps of the blood sample testing method as described above.
The method implemented when the blood sample detection program executed on the processor is executed may refer to various embodiments of the blood sample detection method of the present invention, and details thereof are not repeated herein.
The invention also provides a readable storage medium.
The readable storage medium of the present invention stores a blood sample detection program, which when executed by a processor implements the steps of the blood sample detection method as described above.
The method implemented when the blood sample detection program executed on the processor is executed may refer to various embodiments of the blood sample detection method of the present invention, and details thereof are not repeated herein.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments.
Through the above description of the embodiments, those skilled in the art will clearly understand that the method of the above embodiments can be implemented by software plus a necessary general hardware platform, and certainly can also be implemented by hardware, but in many cases, the former is a better implementation manner. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium (e.g., ROM/RAM, magnetic disk, optical disk) as described above and includes instructions for enabling a terminal device (e.g., a mobile phone, a computer, a server, an air conditioner, or a network device) to execute the method according to the embodiments of the present invention.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. A method for testing a blood sample, comprising the steps of:
acquiring a first threshold value;
obtaining a first scattered photoelectric value of a blood sample;
comparing a first scattered photoelectric value of the blood sample to a first threshold value;
when the first scattered photoelectric value of the blood sample is larger than the first threshold value, judging that the blood sample is a full blood sample with sufficient liquid quantity;
when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, judging that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid;
after the step of determining that the blood sample is a non-whole blood sample or a whole blood sample with insufficient liquid volume when the first scattered photoelectric value of the blood sample is smaller than the first threshold value, the method further includes:
when the blood sample is judged to be a non-whole blood sample or a whole blood sample with insufficient liquid quantity, comparing a first scattered photoelectric value of the blood sample with a preset second threshold value;
and when the first scattered photoelectric value of the blood sample is smaller than the second threshold value, judging that the blood sample is a non-whole blood sample.
2. The method for testing a blood sample according to claim 1, wherein the step of obtaining the first threshold value comprises:
detecting the non-whole blood test samples with different liquid amounts and the whole blood test samples with different liquid amounts by adopting a turbidimetric analysis unit to obtain a second scattered photoelectric value of the non-whole blood test samples with different liquid amounts and a third scattered photoelectric value of the whole blood test samples with different liquid amounts;
and performing statistical analysis according to the second scattered photoelectric value and the third scattered photoelectric value to obtain a first threshold value.
3. The method of claim 2, wherein the step of obtaining the first scattered photoelectric value of the blood sample comprises:
controlling a light source to emit a light beam to irradiate the blood sample in the detection channel so as to enable the light beam to be scattered;
and receiving scattered light corresponding to the light beam through a photosensitive element, and converting the scattered light into a first scattered photoelectric value.
4. The method for testing a blood sample according to claim 1, wherein the non-whole blood sample comprises: serum samples, plasma samples, buffer or buffer-mixed blood samples.
5. The method for detecting a blood sample according to claim 1, wherein the step of determining that the blood sample is a sufficient amount of whole blood sample when the first scattered photoelectric value of the blood sample is greater than the first threshold value further comprises:
and when the blood sample is judged to be a whole blood sample with sufficient liquid quantity, outputting prompt information.
6. The method for testing a blood sample according to claim 5, wherein the step of outputting a prompt message when the blood sample is judged to be a sufficient amount of whole blood sample further comprises:
the blood sample is analyzed by the test unit test.
7. The method of claim 1, wherein the step of determining that the blood sample is a liquid-deficient whole blood sample when the first scattered photoelectric value of the blood sample is greater than a second threshold value and determining that the blood sample is a non-whole blood sample when the first scattered photoelectric value of the blood sample is less than the second threshold value further comprises:
and when the blood sample is judged to be a whole blood sample with insufficient liquid volume, continuously sucking the liquid volume, and executing the step of obtaining the first scattered photoelectric value of the blood sample when the sucking is finished.
8. A blood sample testing device, comprising: a turbidimetric analysis unit and a test unit, the blood sample testing device further comprising: a memory, a processor, and a blood sample testing program stored on the memory and executable on the processor, the blood sample testing program when executed by the processor implementing the steps of the blood sample testing method of any one of claims 1-7.
9. A readable storage medium, on which a blood sample detection program is stored, which when executed by a processor implements the steps of the blood sample detection method according to any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811065182.5A CN109239016B (en) | 2018-09-11 | 2018-09-11 | Blood sample detection method and device and readable storage medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811065182.5A CN109239016B (en) | 2018-09-11 | 2018-09-11 | Blood sample detection method and device and readable storage medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109239016A CN109239016A (en) | 2019-01-18 |
| CN109239016B true CN109239016B (en) | 2021-03-26 |
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| CN1610827A (en) * | 2001-12-28 | 2005-04-27 | 海莫库公司 | Method for quantitative hemoglobin determination in undiluted unhemolyzed whole blood |
| CN101726579A (en) * | 2008-10-17 | 2010-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | Blood test reagent and method |
| CN103837502A (en) * | 2012-11-26 | 2014-06-04 | 希森美康株式会社 | Method for analyzing blood cells and blood cell analyzer |
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| DE102007017906A1 (en) * | 2007-04-17 | 2008-10-23 | Dade Behring Marburg Gmbh | Method for determining the reaction lag phase in an analyte-dependent reaction |
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| CN1610827A (en) * | 2001-12-28 | 2005-04-27 | 海莫库公司 | Method for quantitative hemoglobin determination in undiluted unhemolyzed whole blood |
| CN101726579A (en) * | 2008-10-17 | 2010-06-09 | 深圳迈瑞生物医疗电子股份有限公司 | Blood test reagent and method |
| CN103837502A (en) * | 2012-11-26 | 2014-06-04 | 希森美康株式会社 | Method for analyzing blood cells and blood cell analyzer |
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